Revised Oct. 18, 2000

35 Yeast Mitochondrial Carriers

      Name    Chrom. location        Length         Genbank accession

Chromosome I         

      none

Chromosome II         

      AAC2 (ADP/ATP)  YBL030c    318 amino acids  J04021, M34076, Z35791, X77291
                                                  M34075, X74427, PIR:S31978
      AAC3 (ADP/ATP)  YBR085w    307 amino acids  Z35954, PIR:A36582
      YMC2            YBR104w    329 amino acids  Z35973, X78993
      RIM2            YBR192w    377 amino acids  Z21487, Z36061, PIR:S36081
      CTP1  citrate   YBR291c    299 amino acids  X76053, Z36160, U17503, PIR:S39148

Chromosome III        

      none

Chromosome IV         

YHM1 (formerly SHM1)  YDL198c    300 amino acids  U08352, X83276, Z74246
      carrier         YDR470c    502 amino acids  U33050 (nuc. compl. 30437-31945)
      carrier         YDL119c    307 amino acids  Z74167
                                                  (nuc. compl. 246691-247611)

Chromosome V          

      carrier         YEL006w    335 amino acids  U18530 (nuc. 54732-55739)
      carrier         YER053c    300 amino acids  U18796 (nuc. 40977-41879)

Chromosome VI 

      carrier         YFR045w    317 amino acids  D44597 (nuc. 8113-9068 with 
                                                         frameshift and seq. errors)
                                                  D50617 D44605 (242450-242986)
Chromosome VII        

     carrier          YGR096w    314 amino acids  Z72881 
                                                  (nuc. 676615-677556)
     carrier          YGR257c    366 amino acids  Z73042 
                                                  (nuc. compl 1006204-1007301

Chromosome VIII       

      carrier         YHR002w    357 amino acids  U10555

Chromosome IX         

   FLX1 (FAD carrier) YIL134w    311 amino acids  Z38059 (nuc. 58424-59359)
                                                  Z47047 (nuc. 97395-98327)
                                                  L41168
       carrier        YIL006w    373 amino acids  Z38113 (nuc. 14282-15403),

Chromosome X          

MIR1 (phosphate car.) YJR077c    311 amino acids  J05302, X57478, M54879, Z49577
                                                  L47993 (nuc. compl. 41507-42442)
      MRS3            YJL133w    314 amino acids  X56445, X06239, Z49408, X87371
                                                  PIR:S01267, PIR:S20228
SFC1 formerly (ACR1)  YJR095w    321 amino acids  Z25485, Z49595, T37090 (EST)
Succinate fumarate carrier                        PIR:S36407

Chromosome XI         

OAC1 formerly (PMT1)  YKL120w    324 amino acids  S44213, Z28120, L04948, PIR:S25357
Oxaloacetate carrier
      MRS4            YKR052c    304 amino acids  X56444, Z28277, PIR:S13533

Chromosome XII        

      DIC1            YLR348c    298 amino acids  U19028 (nuc. 34460-35356)
dicarboxylate carrier

Chromosome XIII       

      AAC1 (ADP/ATP)  YMR056c    309 amino acids  M12514, Z49703 PIR:A24849
                                                  PIR:S36419
      YHM2            YMR241w    314 amino acids  Z48756
      carrier         YMR166c    368 amino acids  Z49700 (complement of 29688-30794)
                                                  Z49705(COMP 29688-30794)

Chromosome XIV        

      PET8            YNL003c    284 amino acids  U02536, X77114, Z71279
      carrier         YNL083w    545 amino acids  X89016 (nucleotides 15245-16880
                                 with one frame shift probably near 16444, delete
                                 one G in run of 9) Z71359

Chromosome XV         

ORT1(ARG11)           YOR130c    292 amino acids  X90518 (complement of 40326-41204)
ornithine carrier                                 X94335 (complement of 93450-94328)
      ODC2            YOR222w    307 amino acids  X92441 nucleotides 19114-20037
oxodicarboxylate carrier
      CRC1            Y0R100c    327 amino acids  X94335 (complement of 36818-37801)
carnitine acylcarnitine carrier

Chromosome XVI        

      YMC1            YPR058w    307 amino acids  X67122, M24517, Z49219, PIR:S30141
      carrier         YPR021c    902 amino acids  Z49274 (complement of 117-2825)
      carrier         YPR011c    326 amino acids  Z49919 (complement of 9627-10667)
                                                  U31900 (complement of 27235-28215) Z71255x23
      carrier         YPR128c    328 amino acids  U40829 (complement of 173-1159)
      ODC1            YPL134c    310 amino acids  U43703 (complement of 25148-26080) 
Oxodicarboxylate carrier                          a fragment of this = EST T17520.
......................................................................................

The following 29 yeast carriers have been disrupted or deleted and are viable.
AAC1, AAC2, AAC3, CRC1, CTP1, DIC1, FLX1, MRS3, MRS4, MIR1, OAC1(PMT1), ODC1, 
ODC2, ORT1(ARG11), PET8, SFC1(ACR1), YEL006w, YER053c, YFR045w, YGR096w, 
YHM1(SHM1), YHR002w, YHM2, YIL006w, YMC1, YMC2, YMR166c, YNL083w, YPR128c.
The YHR002w sequence from chromosome VIII has been precisely deleted and 
replaced with green fluorescent protein and HIS3 by the St. Louis yeast 
sequencing group.  The deletion strain is available from them.  No phenotype is 
listed.

RIM2 is the only LETHAL null mutant among the carriers so far.

Five carriers have no information available on their knockouts yet.
YDL119c, YDR470c, YGR257c, YPR011c, and YPR021c 

Growth on non-fermentable carbon source (GLY+) for disruptions/deletions is seen with:
AAC1, AAC3, CRC1(carnitine acylcarnitine carrier), CTP1, DIC1(dicarboxylate 
carrier), MRS3, MRS4, OAC1(PMT1), ODC1, ODC2, ORT1(ARG11), SFC1(ACR1), YEL006w, 
YER053c, YFR045w, YGR096w, YIL006w, YMC1, YMC2, YHM1(SHM1), YHM2, YNL083w

Lack of growth on a non-fermentable carbon source (GLY-) is seen with AAC2, 
FLX1, MIR1, PET8, RIM2, YHR002w

SFC1 (ACR1) cannot grow on ethanol or acetate as sole carbon sources, but it 
does grow on glycerol and pyruvate, so it is respiration competent.

The phosphate carrier MIR1 has a second isoform on chromosome V that is more 
similar to the mammalian phosphate carriers (YER053c).  This is probably also a 
phosphate carrier and may be redundant in function to MIR1.

Overexpression of MRS3 or MRS4 causes temperature sensitive petites.

The carrier on chromosome XVI that is 902 amino acids long has an N-terminal extension 
with no Prosite motifs and no blast homologies.  The closest sequence to the carrier
portion is U00052 from C. elegans that also has a long N-terminal extension with a
calmodulin-like sequence in it. The two carriers are 57% identical and so probably
transport the same substrates in C. elegans and yeast.  In C. elegans this is probably
calcium regulated.  Several overlapping human ESTs have been found that are homologs 
to the C. elegans sequence. There is a second carrier only 17kb toward the centromere.
This one is on Z49919.  These are the closest carriers on the yeast chromosomes.

Carrier YDR470c is quite odd.  It has an N-terminal extension and two insertions 
between the three domains that are not shown in the alignment.  Many of the sequence 
landmarks are missing.