P450s from red algae genome projects

Cyanidioschyzon merolae P450s 5 sequences

Galdiera sulphuraria P450s 8 sequences

These two algal genomes have P450s that cluster into five groups.

#331 is a CYP51 as is contig 1016. #4765 and #4211 are both CYP710 sequences. Both algae seem to have two that are recent duplications, not in the common ancestor. #444 is probably the ortholog of contig 1062. #201 is the probable ortholog of contig 1041.

Contigs 454, 981 and 989 are unique to Galdiera. They cluster together on a tree. Contig 981 and 989 are quite similar (40%) and are a recent duplication. Any orthologs to these sequences are lost in Cyanidioschyzon. The alignment below suggests some revision may be needed to some of the sequences.

Alignment of the 13 sequences (Phylip format)

13 628

331 -------MGT ILAALANQFQ ALLARARDGD TDAIGWLALG VAALVWLMTR

CYP51con10 ---------- ---MLSQDSI ALSTLTSSLE AYCWALVYIL STILFFGILW

4211 -MIRRPRYAL DASGSSRVGG SVARHCLRRA TLRWDRR-LG YRQPCAGVRC

4765 MIETASLTRS TPVAAAEWVV SVARHCLRRA DAALGIAGWG TASRALAYVA

710B_gen ---------- ---------- ---------- MDIVSFNSLS SGNLIILLVV

710B1_like ---------- ---------- -------MQL TEFDSFNKFL SGNLVFLGVS

con981 ---------- ---------- ---------- ---------- -MLQLWIVLV

con989 ---------- ---------M MMSCLAVSLL QLSNLSQDWS RVFKLFILAA

con454 ---------- ---------- ---------- ---MASPNEI ARWFLQTRTK

con1062 ---------- ---------- ---------- ---------- -MWIGLLLFF

444 ---------- ---------- --MFPVFIWF VIVAIAIVTF TFGVPGTLLR

201 ---------- ---------- -----MILAR NLLHVPRPSL YVVAALGAFS

con1041 ---------- ------MSLM KRVMFLGGQV ARWLVNGGLL SLVFVDLAFS

AAQALATLLS PAKPAEGLEL AYPPLYTEGF PVVGNVAAFA R---NPLQLA

RITGSFFLSK LGIAREVKGQ QLPPTYKEGL PLVGNLIAFA K---GPLNVV

AAGARPRDRR QVRLRRWAGY GRIPGPRFVI PLIGSIVEMV L---SPYGFW

LLVLGLAIAE QVRLRRWAGY GRIPGPRFVI PLIGSIVEMV L---SPYGFW

ITMICYFILE QLHYFWWKRS SKLPGPSFTL PFLGSIIEMV K---NPYQFW

IALVCYLLFE QLRYFWWKRS SKLRGPSFTL PFLGSIIEMV K---NPYEFW

TFSCLFLYV- FILPKWRNRH IPGPRPSLLL GNVSELSRQG G---TAPLVF

LFWTVFKFLK YVYPYWRFRN IPGPPPKWPV GNIFELLRKP G---QEHRIL

LHSYLFSYCF MFLAPRIALV SPEAAKHVMV KNVRNYVKPP M---VRQGLS

VLVFTLYLVR QNTCTGNKAN LSYSPVCKGL PLLGSALEFG K---NPLKFL

RQRRKQLRGD TFPVRDVSAE RLPPCIRGWC PYVRAGFQFA FGAAPPQSFL

LFQVGSTVAK YALWFWKQKR AGRPVSRGLL EVVTALGPVP VPG-FGNALY

RWSLEDLWCT PPSQSFNCIG QGRDHSMDHK SKQVIFVYII FDCNLPSLPG

KRAYAQLGDV FTLRVGPKRF TFLVGPRAHE VFFRANDDEL DQG---PVYG

QRGYQSCGDI FTFKVFHKHI TFLVGPKAHE IFFQGTDDEL DQN---EVYA

ERQRRLNPCG LSWNALAGFF VLFVTDTDLS RYVLSENGPH AFE---MILH

EKQRLLDPQG VSANFLVGRI TLFVTDSALV RAILNNNSAR TFL---LALH

EKQRLLDPQG VSANFSLGRI TLFVTDSALV RAILNNNSAK TFL---LALH

ERFRKQYGDV FQIWSFYRQI VVISHPDDIK YIIVTKNFPK A-----EEFN

LQYAKQYGPT FQLWYLNRRT IIVANPEDAK FVLATRNYPK S-----PIFC

N-LLGNKGIL LAEGDDHARQ RRIILPAFHF DALVHLGPIF R--------A

QECRKQYGDV FTVLLPGRRM TFIFAPTQEL RKIFFNGSPN LIS-----FT

EAARKQHGDV FTVEMFGRRM TFLFSKNGIG QFFDSAPSKV SFIRAVEPFT

LSRVGFFRAL YESCLERQVT VFWLSSTPLL IVSAPDLVEQ VLT----SRT

PSPWPIVGNC IPLSSNLYQT LYQYVEQPIS LYFIASTPFV VVTDEAAVRK

FSVPVFGRGV VYDAPLAIRL EQFRFVSTAL RAARLREYVP LMVAEAETYF

FSVPIFGKGV VYDAPLEKRL QQLRIMSAAL RPARMYGYVD QMVLEAVQFF

PNGWRILGRN NIAFKSGEEH KLLRQSFLRL FTPKALGVYV SIQERLIREH

PNGWRILGRN NIAFKSGEEH KLLRQSFLRL FTPKALGMYV SIHERLIGEH

PSARLILGKN NIAFMHGQEH KELRKSFLSL FTRKALGVYL TLQETSIKSH

PSARLILGKN NIAFMHGPEH KELRKSFLAL FTRKALGVYL TLQETTIKSH

LSLSPLAGRG LLTVGKSQHQ ERRRAISKHF NEDFLRQLHR HMRVELMILL

RCFSPLG-HG LLTLSQEEHP VQRKAISQRF NEEFLQSLHH HLTAELEVFM

QGQQVVQRWL NRPEEAIDVH LDMTQVTMNV IALAAFGYDP NTDSGQELYR

AGVEPLTCRI FGISKKGFSM AHRSLLTTLR SELGAKHIPQ LAHRLINRYL

DGIFGLAPTY FEKILHMLLV QLREELHLDK GSDRGLARHL TGFAEALRMA

FEKPQYFGYR SRTVKTALEL HQRAELLREK IETADPDQQQ RVREDPSRAA

VLGSGMYQKP KYFGYRSSTI RYSVEMNQKL ILTNEQMRQQ QADSSRKALK

KRHWHG---- TDGTAD--LL KSLSELIILT ASRCLMGREV REQLFEEVST

RKWGD----- -QGQVD--IL ESLSDLIILT ASRCLMGREV REQLFEKVSK

LAAWLS---- GRGTDGTTAA FEVRPRIRDL NLRTSQTVFV GPYLRD-RER

LAAWLS---- GRGTDGTAAA FEVRPRIRDL NLRTSQTVFV GPYLRD-RER

LQKWIQ---- LSKEN---DE MEMSFLCRDL NLETSQYVFA GPYIGEQRDQ

LQRWIE---- LCKEK---SP LEMSFFCRDL NLETSQYVFA GPYIGERRDE

SKLQQV---- TERKESIDFD KEATSYTLDV MCRTGFGCTA NTQEDA-SHP

AQMDAL---- CDTERVVDLD ALISALTLDV IARTAFGVSF TAQTSQ-HHP

AYRDIF---- TQRPPSRMLA MLFSLLPSWL LQSMPLSRLL RRQQSN-VRL

FTFRTV---- WGKEDEKEAS NLLTETLSDA SLRVIFGDEF ANASPS---L

MRQQMASLDG QAVAHVDDLF DLCGRLIFTA SFQTVFGREC ANALNK-DGR

LLDLIDR--- SLTEIASSTE KFLAELRAED NVGKDASKLI QRFYVQLNCK

VMIDS----- -KVSDIIDGM IEAAEAVVHA VDGREQVENI RRKVIELNLN

LYHQLDQGMQ PLSVFAPHFP CKAHW----- QRDRARREMR RLFASIIANR

LYHDLDQGMQ PISVFAPYLP ISAHR----- KRDKAREEMV QLFRTVIQNR

FCADYLLITQ GFLSFPLALP GTGLW----- RAIRARERVL RDLTACVRAS

FCHWYITVTK AFISAPVFLP GTNLW----- KAYFARKKIV ALLENAVIQS

FCSWYITVTK AFISAPVFFP GTNLW----- KAYFARKKIV ALLENAVIQS

ISRAVNVSLR EMYHNLVAYP IRNCFGLYSS PALKNATGVI REFASQVIEA

MPHAVLTLLD ELVNNMIFYP YRFWLSHITQ KRLNEAINVI RKFCNMVIDL

VKKKVTEIVQ KRREEYEALL VKDSN----- AMG--KSTTN RDLLDMLVAA

FKDFVDFDEW FELAATPLLP HFLLR----- PFVKSRRKLL DTISQNWKYT

LYETFVAFDR EFELAALPIP QFLLR----- SFSRARRQLL RAMQRAVRLV

VLFGLVVDNA EATRIASAIE KAGAEFSR-- RMILPQRALY AWFANVSYIY

VLFGYKNDKD VGSLSHIIFE AGKEFILR-- -TVNPFRIGW RWMANFRFFQ

RKRYQEIAAQ AESVGQDPQQ ALEAAKEVDV LQVFMDSQYR DGS-RLTDDQ

RR-------- ---------- --RNVKEDDM LQTFMDASYR DGS-RPSEYE

KERF----RK DAEAEPQCLL DFWTVSVLEE VAAAKRENRA PPK-YSADHE

KK------YI GNGGTPRCLL DFWTQRVLEE MEEATQQDKE MPS-YSNNRK

KR------YM ADGGSPRCLL DFWTQRVLEE VEEAAQQGR- SVS-YANNRK

RRT------- ------ESEE D-KTRRPLDL LDIFLKMDN- -----LSDQN

RLQ------- ------ESRE E-KSNRVRDL LDIFLESDE- -----TRD-N

RD-------- ---------- --------PE LEKKSSHLP- ----YLTDEE

KN-------- ---------- --------AP IHKLTEAYG- ------NDGN

EP-------- ---------- -------ATP AGKLLAKLD- -----SDEKV

HTS------- --------VL LIFGQKILRH LRISSNSWIN GWLGKAGRLR

YVFS------ ---------L ITIGRRVCQH MDSQPATWVH GWVGKVGKIG

ITGLLIAVLF AGQHTSTITG TWTGLLMLRK PELVTRVRAE QEQVLYDDDG

VAGLLIALLF AGQHTSSITG SWTGMLLLRN KDVFERVKKE QDTIIEEHG-

MADAMLDFLF ASQDASTASL VWTTVLVAER PDVLQRVREE QQRLRPHDE-

MAETLMDFLF ASQDASTASL TWTLALMSDY PDVLKKVQEE QKRLRPNNE-

MAETMMDFLF ASQDASTASL TWTLALMADH PDILKRVQEE QKRLRPNNE-

IIAEIATFLV AGHDTTSHTM SWLIYEVCQH PEIEQKIQQE VDTIWGDRQD

VIAHVATFML AGHDSTSHTL SFCMYEIAQN RDIERKLQEE SD-RFIVAQD

ITSQALTFMA AGQVTTAVLL SWTLFELSIH PSAQEKLRQE LQTMETTLST

VPSLLLSALW ATWSNVSPTS FWTLTHILAD EKAKVKVLAE VEKSCPLLLS

CASVLLTLLW ASSANTLLSA GWLIVLSAEW CNRARPVTES MA--------

RLAKVLGLLM AATQTVPIAA SWALILVSVH EDVREKLACE ARRLLSEDLS

KLGKVVGLIM ASSQTVPTTC LWLLFLLSKY PQVVEKIREE TSRVLHSTKK

C--------- ---------- ---------- --FKIDYDAL LR-LDVMHRC

---------- ---------- ---------- --DELNYDVL SK-MNLLHLC

---------- ---------- ---------- ---PITYELL EQ-MVYTRAV

---------- ---------- ---------- ---PLSFELV ES-MTYTRQV

---------- ---------- ---------- ---PLSFELV EN-MTFTRQV

---------- ---------- ---------- --WMLSFEEI GQ-LEYLNKV

---------- ---------- ---------- --RIVPFDQV GH-LDYTRMV

QD-------- ---------- ---------- --ITEMVQHL DK-LEYLDVV

SK-------- ---------- ---------- --TELSLEWI FSNLPFTAYC

---------- ---------- ---------- ---------- WE-------L

VNGGRGVAQQ GSQTSQTTPA QKANLTASDI ATDVNRLSKL LKKHSYFDAV

QS-------- ---------- ---------- --MEEFTVDD LNELAYVDCV

IKEALRMYPP LIFLMREVVI PRTYRDYV-- -IPKGDIVVV SPPLAMSLPE

IKETLRMYPP LILLMRKVLK PKFYKEYV-- -IPENDIVMV SPAASGRLEN

VLEVLRFRPP PVMVP-QVAS KRVQLPNG-- -YEVPRGALV VPSLWTACMQ

VKEILRYRPP AVMVP-QNAM GSVPLTEN-- -VTVPKGSFV MPSIWSSCMQ

VKEILRYRPP A--------- ---------- ---------- ----------

WKETLRKHPV AATGTLRRLD TDVTLPSCGM LLRKNTAILV PIYLVHRNPE

WNEALRTHPA AANTSVRCAD RDDVLPGSGI PITKGTGLMV SSYLIHHLPQ

LHESLRLHPP VLFITRQAVQ DDEILGFP-- -ISQGAIVNI PIVALHRDPE

VSETLRLYAS VVDIR-KVVE NLEFREFI-- -IRKGDYLCI SPAVSHRETT

VTETLRLASS GIAVR-IGCE PLYVDDFR-- -IPAGDYLCI SPWLAHQDEH

IAETLRLFPP FPLIQRQAQC DTHLGDVF-- -VPGGTLVAA VPWLQHHHPA

VKECLRLYPP FPLLQREPEM DDILENVK-- -IPARTPVYI VPWLLHHHPK

VF-ADPDRYD PDRYA---PP REED--KRVP FSHIGFGGGR HACMGEQFAY

VF-KNPNAWD PDRFG---PN REED--KKAP FSFIGFGGGR HGCMGEQFAY

GF-PSPERFD PERML---PP RQEE--QKYR KHFLTFGCGP HMCVGRNYAI

GF-PDAYKFD PDRMS---PE RQED--IKYR QNFLTFGIGP HVCVGREYAI

---------- ---------- ---------- ---------- ----------

FW-PDPETFE PERFT---RE NTM---KRHP FAFQAFSNGP RNCIGQFFAT

YW-ENPDHFI PERHT---KE AVR---QRSP YYFLPFSRGS RNCIGQFVAN

QWGPDAESFR PERFLSSDKN NVVI--QRHA MAWLPFLYGT RACTGQRFAM

LF-PQSEDFI PDRFQ--KQG THPN--AVFD KDLLTFGGGF YKCPGQSFAM

RG---GKRFD PCRYQHIEDK KALF--RGRT RQLYTFGGGM YRCPGQEYAL

YW-KQAESFN PERWISPTDN VARHGDAPSD YCYIPFGRGR RMCAGNPLAM

YWK-QPEDFI PDRFMY---- NASHGDAPSD FVYIPFGRGN KMCAGYHLAL

LQIKTIWSVI LRDYDLEPVG PL----PLPD YSAMVVGPKP PCLVRWRRRT

LQIKTIWTVL VRSFDLEPIG DL----SQPD YNAMVVGPRP PCLLKYRKKK

NHLMCYLAVL ATTVDWTRVR TV----HSDE IIYLPTIYPA DSVIRARWRV

NNLIAFLALI STECKFQRYR TK----KSDD IIYLPTIYPG DCLMKFV---

---------- ---------- ---------- ---------- ----------

HEALTTLSSL YHFFTFRLAC RA----EDVK PYHAMTMKPS VGKVSEDAKG

HEALTILSTI YKRYEIRLAV GA----QEVE EYFRVTMKPH CRFYVQGKKD

LEAKTILFEL LTKVSVRLQP GC----EVKG YGMVSVPRDV RLQVVDLHKE

VEIVLLIALV FYLYDIQLVD RV----PKMK ESQSVGIKKP SCSCRIHYLW

HELVLFIQIF FEFVERVELG VAGDPLTSMD AYRLVGIKRP TRPFPGKLFL

VELKCLLLLV ALQEPDLLFD LEP---QETS PEPQAGMRFP PLTMRPPRFA

LELKILTIYV CQYYDWKCSF PQ----GKEP VSKKYPIETI THSSCNRFFF

P--------- ---------- --------

DSFLDRVSLY A--------- --------

ADASTGAGTG AGTDTACTSE AVPVVVAS

---------- ---------- --------

--VSEYVKLP VWVTPRNTMA HLREE---

PSLDAHLGLP VKIYSRKCYS --------

---------- ---------- --------

KRRLAGMEEI ---------- --------

ISSCCDLRCG DPHPSERW-- --------

VRQEEPIHRK LCQQNSNEL- --------

IMQLLSIGNV S--------- --------