Hydra

>gnl|ti|647066038 1095898227332 34% to 17A1 35% to 2U1 fugu 33% to 2U1 human

74% to 1095901734433

1097567032902 1096761105127 1096123323522 1096088745900

Combined seq from CN769290 and CN769570 39% to CYP17A

EST = CN769570.1

mate pair of 1096088745900 had partial match to N-TERM. WALKED UPSTREAM TO

1097672588127, N-term still missing, end of this exon seq not certain

cannot walk upstream any further

(1) AFNRNTNSLINSDPGPRFKILRKLASSSLKIYAEGLLGMERIAISEYCELSKKLQSIKEKPVSVHKIM (1)

AGCATTCAACAGAAATACGAACAGCCTCATTAACAGTGATCCAGGCCCGCGTTTTAAAATTTTA

CGAAAGTTAGCATCATCTTCTTTGAAAATTTACGCTGAGGGTTTATTGGGAATGGAAAGA

ATAGCAATCAGTGAATATTGTGAACTGAGTAAAAAGTTACAATCAATAAAAGAAAAACCA

GTATCGGTTCATAAAATAATGGGT

(0) QSTLNIICTILFNHRYEDDNQEFQNIIKYSSLIVQTFNETSYVSS

IPLLRYFPTATSRNIFEIIRLRDPILKRKLQEHRKSYDKNNLRDITDALIKVSLDSEMGE

ELTEKITDDNIEFLLNDFMIAGSETSSSTILWFIVYMLHWPEYQNKLYDEITKVASDNRY

VSLKDRPMLHLMQAAIHETLRLSSVVPLGLVHKAMENSSICGKFVPKGALILTNLWSMHH

DESYWKNAMSFYPERWLEKSGEFNYKLGYAYLPFSNGPRSCLGETLAKTELFVFITRLLK

DYRFEMPTGKELPCLDGRSGITSPPNDFEVVIIPRN*

AGCAAAGCACACTTAACATTA

TTTGTACCATTCTTTTTAATCATCGCTACGAGGATGACAACCAGGAGTTTCAGAATATCA

TAAAATACTCAAGTTTAATCGTTCAAACTTTTAATGAAACCAGTTACGTATCTTCCATTC

CATTGCTGCGCTATTTCCCAACGGCAACGTCGCGAAATATTTTTGAAATCATAAGGCTTC

GTGATCCGATTTTAAAACGAAAACTCCAAGAGCACAGAAAATCTTACGATAAGAATAATT

TACGTGACATAACCGATGCATTAATAAAAGTGTCTTTAGATTCAGAGATGGGTGAAGAAT

TAACTGAAAAGATTACTGATGATAATATTGAGTTTCTTTTAAACGATTTTATGATTGCTG

GATCCGAAACTTCATCAAGTACTATTCTTTGGTTTATTGTTTACATGTTACATTGGCCAG

AATACCAAAATAAACTTTATGATGAAATTACTAAAGTAGCATCAGATAACCGTTATGTAT

CTTTAAAGGATCGACCTATGCTTCATTTAATGCAAGCTGCAATTCATGAAACACTTAGAC

TGTCATCGGTGGTACCTCTTGGTTTGGTTCATAAAGCAATGGAGAACAGTAGCATTTGTG

GCAAGTTTGTTCCTAAGGGAGCTCTTATTTTAACAAATTTATGGAGTATGCATCACGATG

AAAGCTATTGGAAAAATGCAATGAGTTTTTACCCGGAACGTTGGCTGGAAAAATCTGGCG

AGTTCAATTATAAATTGGGGTACGCATATTTACCGTTTTCTAATGGACCTCGTAGTTGTT

TAGGAGAAACATTGGCAAAAACAGAGTTGTTTGTGTTTATTACACGATTACTTAAAGATT

ACCGATTTGAAATGCCAACTGGAAAAGAGTTACCTTGTTTAGATGGTCGTTCTGGAATCA

CCTCCCCTCCTAATGACTTTGAAGTCGTGATAATTCCAAGAAATTAA

>complete combined seq CN566859 CN566581 CYP2 clan member [gene 2]

1097326058990

32% to 2X9 aa 26-146 34% to CYP17A

MFLEVIGAVFIPPLIWTIWVYIKHLIDCLHYPRGPIPLPFIGNGYLIRKAEPYKELVNLGKIYG

DVFSFSVGSVRYVIVNSLEGIQEVLVKKGWQFAGRPKGP ()

SWDRSIHGLIQRDPSKKFKILRKLATSSLKIFADGLAGMESKAIEESFQLNKKLLETNGKPFSMQEIT (1)

1097329249233

(1) TLCVLNIICSILFNHRYKEDDLEFQDIIKYSNICFKERGVNNYIISIPWLRY

FPSASSRNLDEMIKIRDPLL

KKKVQEHKRSYDEYNLRDLTDALIKASNSETGQDPDEKVTDDNIVFILN

NFILAGSETSSNTILWFIVYILHWPEYQDKLYDEILKVTSGSRYPCLKDRPSLHLMQAAI

YETLRLSSVAPFGLHHKAMEKSSICGKSI

PKGALIITNLWSIHHDESYWKNAMSFYPERWLENSGEFNSKLGNAYLPFSSGPRSCIGETL 395

394 AKTELFIFISRLINDFRFVKPILEELPRLEGSFGITCTPYDFKVEIVPRSKNLLV* 227

AGATGCAACTGATAATAAATTCGCGATCCGCTATAAAGAAAAAA

GTCCAAGAGCACAAAAGATCGTATGACGAATATAATTTACGCGATCTAACAGATGCTTTA

ATAAAAGCATCAAACTCGGAGACGGGACAAGATCCGGATGAAAAAGTTACTGATGATAAT

ATTGTATTTATCTTAAATAATTTTATACTCGCAGGATCAGAGACTTCATCAAATACGATT

CTTTGGTTCATTGTTTATATTTTACATTGGCCGGAGTATCAAGATAAACTTTATGATGAA

ATTTTAAAAGTAACATCAGGTAGCCGTTACCCTTGCTTAAAAGATCGCCCGTCACTACAT

TTAATGCAAGCTGCAATTTATGAAACACTTAGGTTGTCATCGGTCGCACCTTTTGGTTTA

CATCATAAAGCCATGGAGAAAAGTAGCATTTGTGGAAAATCTATCCCTAAAGGCGCTCTT

ATAATAACCAATCTATGGAGTATACACCATGACGAAAGCTACTGGAAAAATGCAATGAGT

TTTTACCCTGAACGTTGGTTGGAAAGTTCTGGCGAATTTAACTCTAAACTAGGAAATGCG

TATTTACCATTCTCTAGTGGACCTCGTAGCTGTATTGGAGAAACATTAGCAAAAACTGAG

>1097329039870 1096703377333 1097664213870

MLFKVIGTILIPPLIWVVWIYIKHLVDCLSYPQGPFPLPFIGNAHLIRNRESYKVF

SEFQKIYGSVFGFSIGSTRYVVVNNLEGVQEVLIKKGSQFAGRPRRA (1)

ATGCTCTTTAAAGTCATTGG

TACAATCTTGGTTCCACCTTTAATATGGGTTGTATGGATTTATATCAAACATCTTGTTGA

CTGCTTGTCCTATCCTCAAGGACCATTTCCTCTCCCATTTATAGGAAATGCTCATTTAAT

AAGAAATAGGGAGTCTTATAAAGTGTTTTCTGAATTTCAGAAGATTTATGGCAGCGTTTT

TGGATTTAGCATTGGCTCAACCAGATATGTGGTTGTAAATAACTTAGAAGGAGTTCAAGA

GGTTTTGATCAAAAAAGGTTCACAGTTTGCAGGCCGCCCAAGACGAGCAAGT

>1096703827379 1095896863976

MFPEIVGAIMLPPLIWAAWIYIKHLVDCLVYPRGPFPLPFVGNAYLFSKGKPYKEFVKLG 103

KTYGDVFGFSIGSIRYVVVNSLEGIKKXXXXXXXXXXXXXXXX

ATGTTTCCTGAAATCGTTG

GCGCAATTATGCTTCCTCCCTTGATATGGGCAGCGTGGATTTACATAAAACATCTTGTTG

ACTGTTTAGTTTATCCCCGAGGACCATTTCCACTACCTTTTGTAGGAAATGCATATCTCT

TCAGTAAAGGCAAACCTTATAAAGAATTTGTTAAACTTGGAAAAACTTACGGCGATGTAT

TTGGCTTTAGCATTGGTTCAATACGATATGTAGTCGTGAACAGCTTGGAAGGTATCAAGA

AGT

>1095899160393 frameshifted

MFFEVIRAFFTPPLVWIIMVYIKNLIDYLYYPREPIPLPFIGNGDLIRKAEPFKEL

VNLEKKYGDVFSFRIGLVRFVVVSSLEVILEILVKKGWQANGRPKAP (1)

ATGTTTTTTGAAGTTATTCGCGCCTTCTTTACTCCACCTTTGGTATGGATTATAATGGTTTATATAAAA

AATTTAATCGATTATTTGTATTATCCACGAG

AACCGATACCACTACCATTTATTGGAAATGGTGATTTGATAAGAAAAGCAGAACCGTTTA

AAGAGTTGGTTAACCTGGAAAAAAAATATGGCGATGTTTTTAGTTTTAGGATTGGTTTAG

TCAGATTTGTGGTTGTTTCA

AGTTTAGAAGTAATTTTAGAAATACTAGTAAAAAAAGGGTG

GCAGGCAAATGGTCGTCCAAAAGCTCCAAGT

>1097329360095 4 aa diffs to CN566859 from PKG

FYLNNFILAGSETSSNTILWFIVYILHWPEYQDKLYDEILKVTSGSRYPCLKDRPSLHLM

QAAIYETLRLSSVAPFGLHHKAMEKSSICGKSIPKGALIITNLWSIHHDESYWKNAMSFY

PERWLESSGEFNSKLGNAYLPFSSGPRSCIGETLAKTELFIFISRLINDFRFVKPISEEL

PRLDGSFGITCTPYDFKVEIVPRSKNLLF*

TTTTATCTTAATAATTTTATACTTGCAGGATCAGAGACTTCAT

CAAATACGATTCTTTGGTTCATTGTTTATATTTTACATTGGCCGGAGTATCAAGATAAAC

TTTATGATGAAATTTTAAAAGTAACATCAGGTAGCCGTTACCCTTGCTTAAAAGATCGCC

CGTCACTACATTTAATGCAAGCTGCAATTTATGAAACACTTAGGTTGTCATCGGTCGCAC

CTTTTGGTTTACATCATAAAGCCATGGAGAAAAGTAGCATTTGTGGAAAATCTATCCCTA

AAGGCGCTCTTATAATAACCAATCTATGGAGTATACACCATGACGAAAGCTACTGGAAAA

ATGCAATGAGTTTTTACCCTGAACGTTGGTTGGAAAGTTCTGGCGAATTTAACTCTAAAC

TAGGAAATGCGTATTTACCATTCTCTAGTGGACCTCGTAGCTGTATTGGAGAAACATTAG

CAAAAACTGAGTTGTTTATTTTTATATCCCGATTAATAAATGATTTCCGATTTGTAAAAC

CGATATCAGAGGAATTACCGCGTTTAGATGGTAGTTTTGGCATCACTTGTACTCCTTATG

ACTTTAAAGTTGAAATAGTTCCAAGGAGTAAAAATTTACTGTTTTAA

>1097509039345 92% identical to 1096064108200, probably joins with 1095898835518

1096625274183 1095900033599 1095896933215 100% match so this similar seq is real

1097206379175 1097678021634

MFLEVAFGVVTPLFLYVIATYLDHLFKCRFYPPGPFPLPIIGNLHLIGKKPHEKFVEYSK 538

KYGEVFSLSFGMHRVVIVSGKDSIREVLVQKSNIFAGRPKNYIANIVSRGYKNIGYGDIG 718

PKWKILRKIAHSSLKNYGESTAHLETLVVRESEELHKNLYKKSNRSTKLEHKF (1)

>gnl|ti|649400787 1095898835518 93% identical to 1096064108200, 39% to 17A1 fugu

35% to 2U1

gnl|ti|647175227 1095898288652 1096602038000

(1) GVAVLNVICSIVFGKRYEYENCEFKEILTYMNYVFTGVAGTNAISFIPWLRFLPLDGLR

KLKKGLSIRDPVLRKQLLYHRETYNESNLRDYTDYVIQFSRDEAILKKFGEQLTDDY

LELLLNDIFIAGTETALTTLLWSIIYLIHWPKFQDKIYNEIVSAIGKNRYPSMKDRNMLP

LVNAALSETLRLSSVTPLGVPHKAMEDTTLLNDLKIPKGTTILTNLWQLHHNKNCWENPH

EFNPYRWFTNDQTLDSIKSMNFLPFSAGTRVCLGKGIAEVELFLFYSRLVRDFKFEVKP

GDSLPSLYGNCGLL*

AGGTGTTGCGGTATTAAATGT

CATTTGCTCTATTGTATTTGGAAAACGCTATGAGTACGAAAATTGTGAATTTAAAGAAAT

CCTAACCTACATGAATTATGTTTTTACTGGTGTAGCTGGTACAAACGCAATTTCTTTTAT

TCCGTGGCTTCGTTTCCTTCCATTAGATGGATTACGAAAATTAAAAAAAGGACTTTCAAT

TAGAGATCCGGTTCTTCGGAAGCAGTTGTTATATCACAGAGAGACCTACAATGAAAGTAA

CCTGCGTGACTATACAGACTATGTCATACAATTTTCAAGAGATGAGGCCATCTTGAAAAA

GTTTGGAGAACAGCTAACTGATGACTACTTAGAGCTTTTACTTAATGATATATTTATAGC

TGGAACTGAAACTGCATTGACAACTTTACTTTGGTCAATTATCTACCTTATTCACTGGCC

AAAGTTTCAAGACAAAATTTACAATGAAATTGTTTCAGCTATTGGTAAAAATAGATATCC

TTCTATGAAAGATCGTAATATGCTGCCTCTTGTTAACGCTGCGTTATCAGAAACATTGCG

GTTATCTTCTGTTACTCCATTAGGAGTACCTCACAAAGCTATGGAAGATACAACTCTCTT

GAATGATTTAAAGATTCCCAAAGGCACCACAATTTTAACGAACCTTTGGCAATTACATCA

CAATAAAAACTGTTGGGAAAATCCACATGAGTTTAATCCATATAGATGGTTTACTAATGA

TCAAACACTTGATTCTATAAAATCTATGAATTTTTTACCTTTTTCTGCTGGTACCAGAGT

GTGTTTAGGAAAGGGTATTGCTGAAGTTGAACTTTTTCTTTTTTACTCAAGGCTGGTTCG

TGATTTTAAGTTTGAAGTAAAACCCGGCGATAGTCTTCCAAGTTTATATGGAAATTGTGG

ATTACTCTAA

>gnl|ti|648017453 1095896110991 52 1e-05 35% to 17A1 fugu 34% to 2U1 fugu

gnl|ti|647987527 1095895119635

1096703762277 used this seq to walk upstream past a repeat could not go futher

71% to 1095898227332

(1) ELTTLNIICTILFNQRYEQDDDEFQNIIKYSNLSFKAFSASNLLSSIPWLRYFPTTASKYIQ 707

706 EIERLRDPILKRKLQEHRKSYDENNLRDITDALIKASIHLNAEKDSLIKVTDDNIQFILN 527

526 DLILAGSETSSSTITWFIVYMLHYPEYQDKIFNEVIKVTSGNRYPCLNDRPLLHLLQATI 347

346 HETLRLSSVAPLGLRHKAMENSTICDKPVLKGTLIITNLWSIHHDERYWKNPMSFYPERW 167

166 LNETGEFDYKLGNAYIPFSGGPRACLGETLAKTELFVIISRLVTDFYFEKSVEEDLPRLDSF 374

375 PGVTRSPYDFKVVVVSRS*

>gnl|ti|647193621 1095899233960 1096082123583 1097696262164 1096620040714

1097206342731

Combined seqeunces BP505786 and CB073123 and CB271974 40% to CYP17A [gene 3]

CN570733 same as CN570522 BP505786

50% to 1095898835518 37% to 17A1

(1) VTGVMNVLCGIVFGTQYEENDKELEKVISFKQLILDGVADTFAISFLPWLRFFPSNGLKKVRK

GVLIRDKLLRFQLKKHRETYNPVQIRDYTDYVLKYSKEFETSRNIDEQLSEDNMEMM

LQDIFISGSETTISTLLWFAVYLVNWPKYQDDIYDETIKIVGNDRYPSLSDRPKLHLFES

AMKETLRLSSVIPLGLPHRSLEETSIKKFKIPKNTNVMINLWQLHHDSKSWSDPHTFNPY

RWLNDKNIFDKSKNPNYLPFSTGLRACLGYHTTESIIFLFFTRLIRDFNLCLKPGASTP

SLNGVLRVTLTPDTSYIILKPRSNNLISQKIEA*

AGTTACTGGAGTGATGAACGTTCTTTG

TGGAATTGTTTTTGGTACACAATATGAAGAAAATGATAAAGAACTTGAAAAAGTCATATC

TTTTAAACAGTTAATATTAGATGGAGTAGCAGATACATTCGCAATATCTTTTTTGCCGTG

GTTAAGGTTTTTTCCTTCAAACGGATTAAAGAAAGTACGAAAAGGCGTGTTGATAAGAGA

TAAACTACTTAGGTTTCAATTAAAAAAACATCGAGAAACATACAATCCAGTTCAAATAAG

AGATTACACTGATTACGTACTTAAATACTCAAAAGAGTTCGAAACTTCAAGAAACATAGA

TGAGCAGTTAAGTGAAGATAATATGGAAATGATGCTTCAGGATATTTTCATTAGTGGTAG

CGAAACAACTATATCAACACTTCTTTGGTTTGCTGTTTATTTAGTTAACTGGCCAAAGTA

TCAAGATGATATCTATGATGAAACTATTAAAATAGTCGGTAATGATAGGTATCCTAGTCT

TTCAGATCGTCCAAAGCTTCATTTATTTGAAAGTGCTATGAAAGAAACTCTGCGTTTGTC

GTCTGTCATTCCATTAGGTTTACCTCACAGAAGTCTTGAAGAAACCAGCATAAAAAAATT

TAAAATTCCTAAAAATACAAACGTAATGATTAATCTGTGGCAGTTGCACCATGATAGTAA

ATCTTGGAGTGATCCTCATACATTTAATCCATATAGATGGTTAAATGACAAGAATATCTT

TGACAAAAGCAAAAACCCAAACTATCTTCCATTTTCAACCGGATTAAGAGCCTGCTTAGG

TTATCACACAACCGAATCCATCATTTTTTTGTTTTTTACCCGATTGATAAGAGATTTTAA

TCTTTGTTTGAAACCTGGCGCATCTACTCCAAGTTTAAACGGTGTTTTGCGAGTAACCTT

AACTCCTGATACGTCATACATTATTCTAAC

>gnl|ti|648033522 1095897342515 39% to 17A1 N-term

1095899118747 1095900033599 1096071090512 1096703396910 1096608233968

MFLEIAFGVTAPLLLYVIATYLDHLFKCRFYPP

GPFPLPIIGNLHLIGKKPHEKFVEYSKKYGEVFSLSFGMHRVVIVSGKDSIREVLVQK

SNIFAGRPKNYIANIVSRGYKNIGYGDIGPKWKILRKIAHSSLKNYGESTKHLETLVVK

ESEELHKRLFKNCNRSTELEDEF (1)

ATGTTCTTAGAAATTGCTTTTGGAGTAACAGCTCCTCTGCTTTTGTATGTCATTGCAACTTATCTAG

ATCATTTGTTTAAATGCAGATTTTACCCGCCAGGCCCTTTTCCTTTACCGATTATTGGGA

ACTTACATTTGATTGGAAAAAAACCACATGAAAAGTTTGTAGAATATTCAAAAAAGTATG

GAGAAGTATTCAGTCTAAGTTTTGGAATGCATCGTGTTGTTATTGTTTCAGGAAAAGATT

CTATTAGAGAGGTTTTGGTTCAAAAATCAAACATTTTTGCAGGGCGTCCTAAAAACTACA

TTGCTAATATTGTATCTCGTGGTTATAAAAATATTGGCTACGGAGATATTGGACCTAAAT

GGAAAATTTTGAGGAAAATTGCTCACTCTTCTTTAAAAAACTATGGAGAGTCAACTAAAC

ATTTGGAAACGCTTGTCGTAAAAGAAAGCGAAGAGCTACACAAAAGACTTTTTAAAAATT

GTAACAGATCCACAGAGCTAGAAGATGAGTTTGGT

1096064108200 93% to 1095898835518 1097206931796(9 aa diffs)

1097206498632 walked up to 1096081234652 found mate pair 1096071090512

already known N-term seq matches 1095897342515 100%

1095897342515 38% to 17A1 fugu whole seq.

MFLEIAFGVTAPLLLYVIATYLDHLFKCRFYPP

GPFPLPIIGNLHLIGKKPHEKFVEYSKKYGEVFSLSFGMHRVVIVSGKDSIREVLVQK

SNIFAGRPKNYIANIVSRGYKNIGYGDIGPKWKILRKIAHSSLKNYGESTKHLETLVVK

ESEELHKRLFKNCNRSTELEDEF (1)

(1) GVAVLNVICFIVFAKRYENKDSEFKKILMYMNYVFSGVASTNFASFIPWLRFFPLDGLR

KLKKGLSIRDPVLRKQLLYHRETYNESNLRDYTDYVIQFSRDEAILKKFGEQLTDDYLEL

LLNDIFIAGTETALTTLLWSIIYLIHWPKFQDEIYNEIVSTIGKDRYPSMKDRNMLPLVN

AALSETLRLSSVTPLGVPHKAMEDTTLLNDLKIPKGTTILTNLWQLHHNENCWENPHEFNPYRWF

TNDQALDSIKSMNFLPFSAGTRVCLGKGIAEVELFLFYSRLVRDFKFEVKPGDSLPSLDG

NYGITLTPRIFTTFVVARNDSLVAQNHSL*

>gnl|ti|647182814 1095899213949 1095958075467 1095733042694

1097672545497

54% to 1095898835518, 36% to 17A1 36% to 2U1

walked upstream to 1097672406696 which mate pairs to exon 2 below

(1) GVAVLNVICFIVFGERYQYSDPAFIEILTTINNIVSGLSNTTAVDFLPGLRYLQFSEIK 256

257 KLKSSLVIYFRLLNDQLKKHKKTFDENNIRDFTDSIIKFSKDETMENKFEEELTDEHLEH 436

437 VIGDMFIAGSETTLTSLLWLIIYMIHYPKYQEEIFEEITRVIGENRYPQLSDRDSLHLVK 616

617 ASIKECLRLSSIIPLGVPHKTMSDTTLIGYNIPKNTTVIINHWQIHNDTNHWKNPNEFNP 796

797 HRWIDDDSKFDATRATSYLPFSAGTRVCLGKTVAETELFFFFTRLIRDFKFE

GVPGCPLPSLIGKCSITLAPEEFNVHVTPRINSLMFSKNVLPE*

>combined seq CN774619 CN775634 CYP2 clan member [Gene 1]

32% to CYP1C1 aa 173-297 29% to 17A2

2 ESEELHKRLLMKSKTSVDLKTEFGAAIINVICFIVFGERYQYSNSEFKEVLTTINNIV 175

176 DGLSNTTAVGFLPWLRFLPFSPIKKLSISLSKYIRFLNDKLTKHKETFNENKIRDS 343

344 TDSIIN 361

>1096526199166 frame3_ORF1 7aa diffs to CN774619 may be same gene

(1) GAAIINVICFIVFGERYQYSDSEFKEVLTTINDIVDGLSNTTAVGFLPWLRFLPFSPIKKLSIS

LSKYVRFLNDKLKKHKETFDEKKIRDFTDSIINFSNNEAVKQKFKNVDEHLEPVIGDLFI

TGSETTLTSLLWLILYMMHYPKYQQEIFKEITTVIGEDRYPCLNDRDSLHLVKAALKECL

RLSSIVPLGLPHKTTKETVLMGHSIPGNATVMINHWQIHNDTNYWENPNEFNPYRWIGKD

KKFDPSKATSFLPFSAGTRVCLGKTVAENELFFFFSRLIRDFNFECIPGCPPPSLIGKCN

ITHAPKQFCAYLTPRINNLM*

AGGTGCTGCAATTATAAACGTGATTTGTTTCATTGTTTTTGGGGAAAGATACCAGTATTCAGATTCAGAAT

TTAAAGAAGTTCTTACAACAATAAATGATATAGTCGATGGGTTGTCAAATACAACTGCTG

TTGGATTTTTGCCGTGGTTGAGATTTTTACCGTTTTCTCCAATAAAAAAACTGAGTATTT

CACTTTCAAAATATGTTCGTTTTTTAAACGATAAGTTGAAAAAACATAAGGAAACATTTG

ATGAAAAGAAAATTCGAGATTTTACTGATTCTATTATAAATTTTTCTAATAACGAAGCTG

TCAAACAAAAATTTAAAAACGTTGATGAACATTTAGAGCCTGTGATTGGGGATTTATTTA

TAACGGGTAGTGAGACCACATTAACATCTTTATTGTGGTTAATTCTTTATATGATGCATT

ATCCCAAATATCAACAAGAAATTTTTAAAGAAATTACAACGGTTATTGGTGAAGACCGGT

ACCCATGTTTAAATGACCGTGATTCTTTGCATCTTGTTAAAGCCGCATTAAAAGAGTGTC

TGCGTTTATCTTCAATTGTTCCTCTTGGATTACCACACAAAACAACCAAAGAAACAGTTC

TTATGGGACATAGCATTCCTGGGAATGCAACAGTCATGATTAATCATTGGCAGATTCATA

ACGATACTAACTACTGGGAAAATCCTAACGAATTTAATCCTTATCGGTGGATTGGTAAAG

ATAAGAAATTTGATCCAAGTAAAGCAACAAGTTTTTTACCTTTTTCAGCCGGTACAAGAG

TTTGTTTAGGGAAAACAGTTGCTGAAAATGAACTATTTTTCTTCTTTTCTAGATTAATTC

GAGATTTTAACTTTGAGTGCATACCTGGTTGTCCACCTCCAAGTTTAATTGGTAAATGCA

ATATTACTCATGCTCCAAAACAGTTTTGCGCATACTTGACTCCAAGAATAAACAATCTAATGTAA

>whole gene 1095899272864 1096526199166

MWYEIICGLIISILLYIIGSYLMHLLECRKYPLGPFPIPIFGNLHLLGTEPHKILAAYS

KKYGAVFSISLGLQRIVIISDITTTREALVQKASIFAGRPKSYLIQLISSGYKGIAFMDY

GSFWKVLRKVSHSSLKIYGEGHERFEKILTKESEELHKRLLKKSNNSVELKSEF (1)

GAAIINVICFIVFGERYQYSDSEFKEVLTTINDIVDGLSNTTAVGFLPWLRFLPFSPIKKLSIS

LSKYVRFLNDKLKKHKETFDEKKIRDFTDSIINFSNNEAVKQKFKNVDEHLEPVIGDLFI

TGSETTLTSLLWLILYMMHYPKYQQEIFKEITTVIGEDRYPCLNDRDSLHLVKAALKECL

RLSSIVPLGLPHKTTKETVLMGHSIPGNATVMINHWQIHNDTNYWENPNEFNPYRWIGKD

KKFDPSKATSFLPFSAGTRVCLGKTVAENELFFFFSRLIRDFNFECIPGCPPPSLIGKCN

ITHAPKQFCAYLTPRINNLM*

>gnl|ti|655005893 1095958068757 44 0.002 43% to 4V5 fugu 36% to 4T5

gnl|ti|651153924 1095901025079 N-term

gnl|ti|651153911 1095901025066

1097206604076 1097206339312

complete gene no introns ESTs = CV566433.1 CX054637.1 CV566166.1

MVSVFYILFSGLVFYVVSKILWKLWRNSYGLSSIVTPPNVPFFGTSLYLHSDA

RKFFFQLYDYTRRYGDVFCIWLGPKPVICSSSVKFSEAVLSSQKVITKGFSYDFLHDWLK

TGLLTSTGSKWKTRRRLLTPSFHFSILNNFIKIFEEQASILVDKLAVAADNKEVVDVQVP

IGLATLDIICETSMGVKVNAQSHPDSEYVKAITVLNEEIQMRQKFPWLWFDAIYKLL 568

567 PCGKRFYKALDVAHKLSFDVINERMQMKIQESYCETASDEKKFFLDLLLDIYRKGKI 397

396 DTEGIQEEVDTFMFEGHDTTSAALGWTLWLLGKNPDVQKKLHKEIDEIELNGGSLYDKVR 217

216 QSKYLEIILKESLRMHPPVPMYGRTVEEDMTIDGQFVPKGAQIVLLVLILHSNPDYWEN 40

39 PNDFIPERFEADSYEKRNPYSYVPFSAGPRNCIGQKFAMIEEK

ILLYSIMKNFHLKSMQNENEVFGTLDIIHKSINGINIKFTRR*

ATGGTATCAGTTTTTTATATATTATTTAGTGGACTT

GTTTTCTATGTTGTTAGTAAGATATTGTGGAAGTTATGGAGAAATTCATATGGTTTATCA

TCAATAGTTACACCTCCAAATGTACCATTTTTTGGAACATCTTTGTACTTGCATAGTGAT

GCCCGCAAATTTTTTTTCCAACTATATGACTACACAAGAAGATATGGCGATGTGTTTTGC

ATTTGGTTGGGGCCAAAACCAGTAATATGTTCTTCCTCTGTAAAATTCTCAGAAGCAGTA

TTAAGTAGTCAGAAAGTTATCACCAAAGGATTTTCTTATGATTTTTTGCATGACTGGTTA

AAAACTGGGTTACTTACAAGCACAGGATCAAAATGGAAAACACGTAGAAGGCTACTAACT

CCAAGTTTTCATTTTTCTATACTCAATAACTTTATTAAAATATTCGAAGAGCAAGCATCC

ATTCTGGTGGACAAACTAGCTGTAGCTGCTGACAACAAGGAAGTTGTAGATGTGCAAGTA

CCTATTGGTTTGGCAACCTTGGATATAATCTGCGAAACTTCAATGGGTGTAAAAGTAAAT

GCACAAAGTCATCCAGATTCTGAGTATGTTAAAGCT

ATCACAGTTTTAAATGAAGAAATTCAAATGCGTCAAAAGTTTCCTTGGCTTTG

GTTTGATGCCATTTACAAACTGTTGCCTTGTGGGAAAAGGTTTTATAAGGCTTTAGATGT

TGCTCATAAGCTATCTTTTGATGTAATAAATGAACGCATGCAAATGAAAATTCAAGAATC

TTATTGTGAGACTGCGTCAGATGAAAAGAAATTTTTTTTAGATTTATTGTTAGATATATA

TCGCAAAGGTAAAATTGACACTGAAGGTATTCAAGAAGAAGTTGATACTTTTATGTTTGA

AGGTCATGATACAACTTCAGCTGCACTAGGTTGGACTCTTTGGTTGTTAGGAAAAAATCC

AGATGTTCAAAAAAAGCTGCACAAAGAAATTGATGAGATAGAGTTAAATGGAGGTTCACT

TTATGATAAAGTCAGACAGTCTAAATACCTTGAAATTATTCTTAAAGAATCATTACGAAT

GCATCCTCCTGTCCCTATGTATGGAAGAACAGTTGAGGAAGATATGACTATTGATGGTCA

GTTTGTTCCCAAAGGAGCACAAATAGTTCTTTTAGTTTTAATCTTGCACTCAAACCCTGA

TTATTGGGAAAACCCAAATGATTTTATACCTGAACGT

TTTGAAGCTGATAGTTATGAAAAGCGCAACCC

ATACAGTTATGTACCTTTTTCTGCTGGACCAAGGAATTGCATTGGCCAAAAATTTGCCAT

GATTGAAGAGA

AAATATTACTGTATAGCATAATGAAAAACTTCCATCTTAAGTCAATGCAGAATGAAAATG

AGGTTTTTGGTACTCTTGATATAATTCATAAGTCAATTAATGGAATTAATATAAAGTTCA

CAAGAAGATAA

>1096064105622 very similar to 1095958068757 varies at N-term 86%

346 MIYASYLVLVGLFVFFVSKILWKLWKSSYGLETIATPPNIPVFGTSLYLHSDARKFFFQL 525

526 SEFTKKYGTVFCIWLGPKPMIISSSVKFSEAVLSSQKVITKGFSYDFLHDWLKTGLLTST 705

706 GSKWKTRRRLLTPSFHFSILNNFIKIFEEQASILVDKLAVAADNKEVVDVQVPIGLATLD 885

886 IICETSMGVKVNAQSHPDSEYVKAITVLNEEFVMRIKYPWXLWFDVIYKLLPCGKR

34 aa gap between these two seqs

>CV564924.1 EST 93% to 1095958068757

EKKFFLDLLWDIYRKGEIDTEGIQEEVDTFMFEGHDTTSAALGWTLWLLGKNPDVQRKLHKEIDEIE

LNGGSLYDKVRQSKYLENILKESLRMHPPVPMYGRTVEEDMTIDDQFIPKGAQIILLVLM

LHSNPEYWENPNDFMPERFEADSYEKRNPYSYVPFSAGPRNCIGQKFAMIEEKILLYSIM

KNFHLKSMQDENEVFGTVDVIHKSINGINIMFTRR

GAAAAGAAATTTTTTTTAGATTTGTTATGGGATATATATCGAAAAGGTGAAATTGACACTGAAGGTATTCAAGAAGAAGTTGATACTTTTATGTTTGAAGGTCATGATACTACTTCAGCTGCACTAGGTTGGACTCTTTGGTTGTTAGGAAAAAATCCAGACGTTCAAAGGAAGTTGCACAAAGAAATTGATGAAATAGAGTTAAATGGAGGTTCACTTTATGATAAAGTTAGACAGTCTAAATACCTTGAAAATATTCTTAAAGAATCATTACGAATGCATCCTCCTGTCCCTATGTATGGAAGAACAGTTGAGGAAGATATGACTATTGATGATCAGTTTATTCCCAAAGGAGCACAAATTATTCTTTTAGTCCTAATGTTGCATTCGAACCCAGAATATTGGGAAAATCCAAATGATTTCATGCCTGAACGTTTTGAAGCTGATAGTTATGAAAAGCGCAACCCATACAGTTATGTACCTTTTTCTGCTGGACCAAGGAATTGCATTGGCCAAAAATTTGCCATGATTGAAGAGAAAATATTACTGTACAGCATAATGAAAAACTTCCATCTTAAGTCAATGCAGGATGAAAATGAAGTATTTGGGACTGTTGATGTAATCCATAAATCAATTAATGGAATTAATATAATGTTCACCAGAAGAAAAGGAAAAACTTATCTTGTTTAGTTTAGTTCATTATTTATCAGTAATTTGAAATAAT

>1096064105622 90% to 1095958068757 varies at N-term

1096071088011 joins CV564924.1 EST

MIYASYLVLVGLFVFFVSKILWKLWKSSYGLETIATPPNIPVFGTSLYLHSDARKFFFQL

SEFTKKYGTVFCIWLGPKPMIISSSVKFSEAVLSSQ

KVITKGFSYDFLHDWLKTGLLTSTGSKWKTRRRLLTPSFHFSILNNFIKIFEEQASILV

DKLAVAADNKEVVDVQVPIGLATLDIICETSMGVKVNAQSHPDSEYVKAITVLNEEFVMR

IKYPWLWFDVIYKLLPCGKRFYKALDVAHKLSFDVINERMQMKIRESYCETASDEKKFFL

DLLLDIYQKGEIDTEGIQEEVDTFMFEGHDTTSAALGWTLWLLGKNPDVQRKLHKEIDEI

ELNGGSLYDKVRQSKYLENILKESLRMHPPVPMYGRTVEEDMTIDNQFIPKGAQIILLVL

MLHSNPEYWENPNDFMPDRFEADSYEKRNPYSYVPFSAGPRNCIGQKFAMIEEKILLYSIM

KNFHLKSMQDENEVFGTVDVIHKSINGINIMFTRR

>gnl|ti|655009968 1095963046224 42 0.010 46% to CYP20 35% to 27B1

419 DGGIHKFLVENHKRLGPMFSFYWGKELAVSLACPILFKEVATLFNRP 559

>gnl|ti|646849327 1095897329284 1097672251908 mate pair = 1097672200068 has exon 2

40% to 2X2 N-term

MLLQITCGFLFPPLIWIVWTYIKHLYDCLSYPQGPIPLPFIGNAHLLRKGEPYKELVNLGKIYGDVFGFSIGSIRYVVVNNLEGIKEVLIKKGSQFAGRPRLKFTI (1)

ATGCTTCTTGAAATTACTTGTGGGGTTCTGTTCCCACC

GTTAATATGGATTGTCTGGACATATATTAAACATCTTTATGATTGTTTGAGTTATCCACA

AGGACCAATACCACTGCCATTTATAGGAAATGCTCATCTTTTAAGAAAAGGTGAACCTTA

CAAGGAATTAGTTAATCTTGGAAAGATATATGGTGATGTTTTTGGATTTAGTATTGGTTC

AATTAGATATGTAGTTGTAAACAATTTAGAAGGTATTAAGGAAGTTTTGATTAAAAAAGG

TTCACAGTTTGCTGGTCGTCCAAGGCTAAAGTTTACTATTAGT

Exon 2 1097331043073 1097206900216 1097672200068 mate pair = 1097672251908

This mate pair has 2 aa diffs to 1095897329284

one nuc diff same aa seq

1096124035195 1096041114543 1097329360644 1096625189581

1095958061778 1095898207031

(1) ALSRGMNGLIMSDPSPHFRILRKLASSSLKIYAEGLDGMEKKAINEYSYLHKKLSTMNGKAVSLKRMI (1)

AGCTTTGAGTAGGGGTATGAATGGCCTTATTATGAGTGATCCT

TCACCACATTTTAGAATTTTACGAAAATTAGCATCATCTTCGTTAAAAATTTATGCTGAA

GGATTAGACGGGATGGAAAAAAAAGCTATAAATGAGTACAGTTATTTGCATAAAAAATTA

TCAACAATGAATGGAAAGGCTGTATCTTTAAAAAGAATGATAGGT

>1096124019772 related exon 2 5 aa diffs to 1097331043073

1096123858905 1096123680637

(1) ALTRAMNGLIISDPSPHFKILRKLASSSLKLYAEGLDGMEKKAINEYSYLHKKLSTMNGKAVSLKRMI (1)

>1097265020030 new N-term weak with frameshifts and a stop codon

no exact matches exist so this may be poor quality sequence

TCGCTYPQKIWNVL

WTDIKHLSDSESYPQGPISLPI

XXXAHIERKGETYREIDRLR*IYGDDIGMCIGTLRYVDVNNLEGIRDVLIYTGTQFL

ACGTGTGGGTGTACGTACCCACAGAAAATATGGAATGTC

TGGACAGATATAAAACATCTCTCAGATAGTGAGAGTTATCCACAAGGACCAATATCACTGCCAATT

GCACATATAGAAAGAAAAGGTGAGACATACAGGGAGATAGATAGACTTAGATAG

ATATATGGTGATGATATAGGTATGTGTATCGGTACACTTAGATATGT

AGATGTAAACAATTTAGAAGGTATTAGGGACGTTTTGATTTACACAGGTACACAGTTTCT

CTGGT

>1096110062131 related exon 2 73% to 1095897329284

1097331675401 1097646001099 1096704247756

(1) AWSRALNGLVACDPGPRFKVLRKLASSSLKIYAEGLDGMEKKAADEYSHLNKKLQTMNGKPVSLQNMI (1)

mate pair of 1097646001099 = 1097664041480, continues on 1096703402618

1097329754969 1097664053056

possible frameshift at NDRP_LHL

(1) ELGTLNIICTILFNHRYEEDDKEFQDIIKYSNLTVKIFGGTSILSSIPWLRFLPSASSRSIYE

IVRIRDPLLKKKLQEHKSSFDENNLRDVTDVLIKVSLGSDIAKGSEEKITDENIEFLLND

FIIAGSETSSSTILWFIVYLLHWPEYQDKLYNEIIKVTSGKRYPCLNDRP ?

LHLTQATIHETLRLSSVGPLAIVHKAMENSSICGKPVPKGAFILTNLWSTH

HDESYWKNPMCFYPERWLEKSGEFNSKLGYAFLPFSGGPRSCLGEALARTELFVFFSRLV

TDYRFEKPNGEELPRLNGRFGLTCSPFDFKSVVVPRC*

AGAGTTAGGTACCCTCAACATCATTTGTACTATTTTGTTCAATCATCGATATGAAGAAGAT

GATAAAGAATTTCAGGATATCATCAAATACTCAAATCTGACTGTTAAAATTTTTGGTGGA

ACAAGCATTTTATCTTCTATTCCATGGCTGCGTTTTTTACCATCAGCTTCTTCAAGAAGC

ATATATGAGATAGTAAGAATACGTGATCCACTTTTGAAAAAAAAGCTACAAGAGCACAAG

AGCTCGTTTGATGAGAATAACTTACGTGATGTGACTGATGTATTAATTAAGGTTTCTTTG

GGTTCAGATATTGCAAAAGGTTCCGAAGAAAAAATTACTGACGAAAACATAGAGTTTCTT

TTAAACGATTTCATAATTGCCGGATCAGAAACTTCATCAAGTACAATTCTTTGGTTTATT

GTTTATCTTTTACATTGGCCAGAATACCAAGATAAACTTTATAACGAAATTATAAAAGTT

ACATCAGGTAAGCGTTACCCATGTTTAAACGATCGCCCc

CTTCATTTAACGCAAGCCACAATTCATGAAACACTTCGATTGTCATCAGTAGGTCCTC

TTGCTATAGTTCATAAAGCGATGGAAAACAGTTCCATATGTGGAAAACCAGTTCCCAAAG

GAGCTTTTATACTAACAAATTTATGGAGTACACATCATGATGAAAGTTATTGGAAAAATC

CAATGTGTTTTTATCCAGAACGTTGGTTAGAAAAATCTGGTGAGTTTAATTCTAAGTTAG

GGTATGCATTTTTGCCGTTTTCAGGCGGACCTCGTAGCTGTTTAGGAGAAGCACTTGCAA

GAACAGAGTTGTTTGTCTTTTTTTCACGATTAGTAACAGATTATCGGTTTGAAAAACCAA

ATGGTGAGGAGTTACCGCGTTTGAATGGTCGTTTTGGTCTCACTTGCTCTCCTTTTGACT

TTAAATCGGTGGTTGTTCCAAGATGTTAA

>1097206642797 related exon 2 61% to 1095897329284

1096761288099 1096082164704 1097567110690 1097672343044

(1) DWSRTMNSLINNDLNATFKVLRKITSSSLKIYAEGLVGMEKRAIEEYTHLNKKLLSLKGQAVSIKNMI (1)

AGATTGGAGTAGAACAATGAACAGCCTCATCAATAACGACTTAAATGCAACCT

TTAAAGTTTTACGAAAAATAACATCCTCATCATTAAAGATTTATGCGGAAGGATTGGTGG

GAATGGAAAAAAGAGCTATTGAGGAATACACCCACTTAAATAAAAAGCTTTTATCATTGA

AAGGGCAAGCAGTATCTATTAAAAACATGATTGGT

>1097206059080 5 aa diffs to 1095898809307 might be the same gene

(1) GPCKPSHIICTILFNHRYDENDQEFQDIIKYSNLSVRASSATSLISSIPWLRFFPSTASR

NIYEIIRLRDPILKRKLQEHRSSYDENNLRDVTDSLIKVSLDSALENNSHEKITDDNIEF

LLNDFIIAGSETSSNTVLWFIVYMLHWPEYQDKLYNEILKITSGNRYPCLSDRPMLHLMQ

AAIHETLRLSSVAPLGVGHKAMESSSICGKPVPKGAFILTNLWSIHHDETHWNNAMSFYP

ERWLEKSGEFNLKLGEAYLPFSSGPRSCLGETLAKIELFVFISRLVKDYRFEKPTEEDLP

NLKGESGITRTPSEFKVMAIPRN*

AGGGCCGTGCAAACCGTCTCACATAATTTGCACAATACTTTTTAATCATCGATATGATG

AAAATGATCAAGAATTTCAAGATATCATAAAATATTCAAATTTGTCTGTTAGAGCATCTA

GTGCAACCAGTCTTATATCTTCTATTCCATGGTTACGGTTTTTTCCTTCAACTGCTTCAA

GAAATATTTATGAAATAATAAGACTTCGTGATCCGATTTTGAAACGGAAACTTCAAGAAC

ACCGAAGTTCTTATGATGAAAATAATTTACGCGATGTGACTGATTCCTTAATAAAAGTCT

CTTTGGATTCAGCATTGGAAAACAATTCACATGAGAAAATCACAGATGATAACATTGAGT

TTCTTTTAAACGATTTTATAATTGCTGGATCAGAAACGTCGTCAAACACTGTTCTTTGGT

TTATTGTTTATATGTTGCATTGGCCAGAATATCAAGATAAACTTTATAATGAAATTTTAA

AGATAACATCCGGAAATCGTTATCCATGTTTAAGCGATCGCCCTATGCTTCATTTGATGC

AAGCTGCAATTCATGAAACACTTAGACTGTCGTCAGTAGCACCTTTGGGTGTAGGTCATA

AAGCAATGGAAAGCAGTAGCATCTGTGGTAAACCTGTTCCAAAGGGTGCTTTTATATTAA

CAAACTTGTGGAGCATACATCACGATGAGACTCATTGGAATAATGCCATGAGTTTTTATC

CAGAACGTTGGCTGGAAAAATCTGGTGAGTTTAATTTGAAACTTGGTGAAGCGTACTTAC

CATTTTCAAGTGGACCGCGTAGTTGTTTGGGAGAAACATTAGCTAAAATTGAATTGTTTG

TATTTATATCACGGTTAGTAAAAGATTATCGGTTTGAAAAACCAACTGAAGAAGACTTAC

CAAACTTAAAAGGTGAATCTGGCATAACTCGCACTCCTTCTGAATTTAAAGTTATGGCTA

TTCCAAGAAATTAA

>gnl|ti|649393684 1095898809307 45% to 17A1 C-term. No exact matches

(1) VYLKLGEAYLPFSSGPRSCLGEALAKIELFIFISRLVKDYRFEKPTEEELPNLKGESGITRIPSEFKVMTIPRN*

AGTTTATTTGAAACTTGGTGAAGCGTACTTACCATTTTC

AAGTGGACCGCGTAGTTGTTTGGGAGAAGCATTAGCAAAAATAGAGTTGTTTATATTTAT

ATCACGGTTAGTAAAAGATTATCGGTTTGAAAAACCAACTGAAGAAGAGTTACCAAACTT

AAAAGGTGAATCTGGCATAACTCGCATTCCTTCTGAATTTAAAGTTATGACTATTCCAAGAAATTAA

>gnl|ti|646968536 1095898162561 83% to 1095897329284 37% to 2X2 N-term

1096041100060 1097672010393 1096602125478

MILKVIGSIFFPPLIWFVYSYIKHLIECLYYPKGPVPLPFIGNTNLLRKKETCKEFVNLGKIYGDIFGFSIGSIRYVIVNNLEGIHEVLIKKGSQFSGRPRII (1)

ATGATTCTTAAAGTCATTGGTAGCATTTTTTTC

CCGCCTCTTATTTGGTTTGTCTACAGTTACATCAAACATCTTATAGAATGTTTGTACTAT

CCGAAAGGACCAGTTCCTCTACCGTTCATAGGAAATACAAACTTATTAAGAAAAAAGGAA

ACTTGTAAAGAGTTTGTTAATCTTGGGAAGATATATGGTGATATTTTTGGATTCAGCATT

GGTTCTATTAGATATGTAATTGTTAACAACTTAGAAGGTATTCATGAAGTTTTAATTAAA

AAAGGCTCACAATTTTCTGGTCGACCAAGGATTATATGT

>1097509072583 new exon 3 boundary wrong

(0) LWSYTCDKESGTNLTVLDDLSNLSFDIVGDVGFGYQFNTITSHSSNEFTSAVRNLTKMQI 694

NASVFSKVLITCFPFLVKFLLLFGKRRNLIQIVYKTLNK (2)

AGCTTTGGTCATATACATGCGATAAAGA

AAGTGGGACAAACCTAACTGTTCTGGATGATTTGTCTAATCTGTCATTCGATATAGTTGG

TGATGTTGGTTTTGGGTACCAATTTAACACAATCACTTCTCATTCTAGTAATGAATTTAC

TTCAGCTGTTCGGAATTTGACTAAAATGCAAATCAATGCTAGTGTGTTCTCAAAAGTTTT

AATAACTTGTTTTCCATTTTTGGTCAAATTCTTGTTATTGTTTGGAAAGCGTAGAAATCT

TATACAGATTGTTTATAAAACTTTGAACAAGT

>gnl|ti|648014530 1095896049543 41% to CYP21

LKYLDCVVK

PANTILRTHVSSIHMNETIYPDPHSFKHERFMTG

AGTCCTGCAAATACAATCC

TACGAACTCATGTTAGCAGTATACACATGAATGAAACTATTTATCCAGATCCTCATTCAT

TTAAACATGAAAGGTTTATGACAGGT

>1096082202706 probably the same as 1095896049543 which has errors

1097664076692 1095994179331

(0) NIEVQEKLREDIQKNILDVNNISFEEVMSLKYLDCVVKETLRLHGPAPLLGRRTISATKF

GEYEVPANTILRTHVSSIHMNETIYPDPHSFKPERFMT (1)

AGAACATAGAAGTTCAAGAGAAACTTAGAGAAGATATCCAGAA

AAACATATTGGATGTAAATAATATTTCTTTTGAGGAAGTTATGAGTTTAAAATATTTGGA

TTGTGTCGTTAAAGAAACCTTGCGCTTACATGGACCTGCACCACTTTTAGGCAGAAGGAC

CATTAGTGCAACAAAATTTGGTGAATATGAAGTTCCTGCAAATACAATCCTACGAACTCA

TGTTAGCAGTATACACATGAATGAAACTATTTATCCAGATCCTCATTCATTTAAACCTGA

AAGGTTTATGACAGGT

>1097309000937 1097206907008 1095901911044

MFLVCLALIVLFIGLFLLCYLLKRTFHPLRLLPSPKEQLITGHNRYFHGRDHTSTYLSFN 858

EKFKEEGLCTLDTLY (1)

ATGTTTCTAGTATGTCTAGCACTCATAGTTTTATTTATTGGATTA

TTTTTACTGTGTTATTTATTAAAACGTACCTTTCACCCTCTTCGACTTTTACCATCACCA

AAAGAACAACTTATTACTGGTCATAATAGGTACTTTCACGGCCGCGACCATACTAGCACC

TATTTGAGTTTCAACGAAAAGTTTAAAGAAGAAGGTTTATGTACGCTAGATACATTATATGGT

>1096091465110 88% to 1097331817678

1096625274441 1096123742264 1097265046825 1095964362241

MFLICLALLILSIGLFFLRYLLKRIFHPLQLLPSPKEQLITGHISHFQGRDHSNTFLGF 701

NEKFKEEGLCTLDTLY (1)

>1097331817678 1096526275245 1096124165677 1096110023112 1096761988512

1096701884902

walked down from end of 1096526275245

walked farther from end of 1097672563082 ran into a repeat region

MFVICLALITLFIGLFFLRCLLKRIFHPLRLLPSPKEHLITGHISHFQGRDHSNTFLSFNEKFKEE

GLCTLDTLY (1)

ATGTTTGTGATATGTCTAGCACTCATAACTTTGTTTATTGGATTATTTTTCCTGC

GTTGTTTATTAAAACGTATCTTTCACCCTCTTCGATTATTACCATCACCAAAAGAACATC

TCATTACTGGTCATATTAGTCACTTTCAAGGCCGTGACCATTCTAACACCTTTTTGAGCT

TCAACGAAAAATTTAAAGAAGAAGGTTTATGCACGCTAGATACATTATATGGT

>1095899139433 1096703930092 1097509100606 1097675339850 new exon 2

VPRYVYLIAPEFIKKIFADGKLFQRPTTLKILAPLIGNSMLGSNYEDHHWQRKLFNGAFT 549

SQQLKNYFPAFLKHTNLLMK

AGTGCCCAGGTATGTTTATCTAATTGCTCCAGAATTCATTAAAAAGATATT

TGCAGATGGGAAACTTTTTCAAAGGCCTACTACATTAAAAATCTTGGCACCATTAATTGG

AAACAGCATGCTTGGTTCAAATTACGAAGACCATCATTGGCAAAGAAAGTTATTCAATGG

AGCATTTACTTCACAACAGCTGAAAAATTATTTTCCTGCATTTTTAAAGCATACTAATTT

GCTAATGAAAGT

>new exon 2 1095899339221 1097206043402 1097672369437 possible frameshift/insertion

(1) GFKFIYLLMPEYIKTMVSNGKVFQKSTAMKVIFPLVGNGMLVSNYEHHHWQRKLFNEAFS

AQQLKKYFPAFKEHT DLLIK (0)

AGGGTTCAAATTTATTTACCTTTTAATGCCAGAATATATTAAAACAA

TGGTTTCTAATGGCAAGGTTTTTCAAAAATCGACTGCAATGAAAGTTATATTTCCTCTAG

TTGGCAACGGTATGCTTGTGTCAAATTATGAACATCACCATTGGCAAAGAAAATTATTTA

ATGAAGCATTTTCTGCACAACAGTTAAAAAAATATTTTCCTGCATTTAAAGAGCATACTA

ATAAAAGATTTACTAATAAAAGT

>1095964240637 1097516021618 1096705343938 1095900018167 1096607016658 new exon 2

(1) GFRFVDLLLPEFIKTIFSDGKVFHRSNVLKVLFPLVGNGMIVSNYEDHHWQRKVLNEAFT 854

SQQLKNYFPAFTLHTDLLMK (0)

AGGTTTCAGATTTGTTGATCTATTATTGCCAGAATTTATTAAAACAA

TATTTTCTGATGGTAAAGTTTTTCACAGATCGAATGTTTTGAAAGTTTTGTTTCCTCTAG

TTGGAAATGGTATGATTGTATCAAATTATGAAGATCATCATTGGCAAAGAAAAGTTTTAA

ATGAAGCTTTTACCTCCCAACAGCTAAAGAATTATTTTCCTGCTTTTACATTGCATACTG

ATTTGCTAATGAAAGT

>1097675832709 new exon 1 with one possible frameshift or there is another exon

MCMVYIAVLILLCLIVFF

ANVLKRFYHPLRNFPSPQENLITGHYSYFYRYDHVKTLLNFGKQFEKNGLYTLDTLN (1)

ATGTGTATGGTTTATATAGCAGTATTGATTTTAT

TATGTTTAATAGTATTCTTTGCTAATGTTTTAAAGCGTTTTTATCATCCGCTTCGTAAT

TTTCCCTCACCTCAAGAAAATTTAATTACAGGCCATTATAGCTATTTTTATCGTTATGAT

CATGTCAAGACTTTGTTAAATTTTGGAAAGCAGTTTGAAAAGAATGGCTTATATACATTA

GATACATTAAATGGT

N-terminal EST sequences for hydra P450s

>DN812964.1 ACAC-aac48b12.g1 Hydra EST UCI 7..same as DN812371.1

MYTIGIAVLIFLCFSLFFANILKRFYHPLRKLPSPKENFFTAHYGYFNGYDQINAVINFG 280

KQFKERGLYTLDTLN

>DN810769.1 ACAC-aac19b13.g1 Hydra EST UCI 7.. same as DN812371.1

MYTIGIAVLIFLCFSLFFANILKRFYHPLRKLPSPKENFFTAHYGYFNGYDQINAVINFG 256

KQFKERGLYTLDTLN

>DN816152.1 ACAC-aac24b14.g1 Hydra EST UCI 7.. same as DN812371.1

IAVLIFLCFSLFFANILKRFYHPLRKLPSPKENFFTAHYGYFNGYDQINAVINFGKQFKE 199

RGLYTLDTLN

>CN775805.1 tae77f11.x1 Hydra EST Darmstadt .. same as DN812371.1

IAVLIFLCFSLFFANILKRFYHPLRKLPSPKENFFTAHYGYFNGYDQINAVINFGKQFKE 185

RGLYTLDTLN

>BP514308.1 BP514308 Hydra magnipapillata c...have this one

MYSIYIAIIIVPLVFFVAVFFKRFYHQFRLLPSPKESLITCHYSYFDVHDHVNTLLNFG 208

KEFKDYGLYTINTL

>BP514307.1 BP514307 Hydra magnipapillata c...same as BP514308.1

MYSIYIAIIIVPLVFFVAVFFKRFYHQFRLLPSPKESLITCHYSYFDVHDHVNTLLNFG 208

KEFKDYGLYTINTL

>BP505238.1 BP505238 Hydra magnipapillata c... same as BP514308.1

MYSIYIAIIIVPLVFFVAVFFKRFYHQFRLLPSPKESLITCHYSYFDVHDHVNTLLNFG 209

KEFKDYGLYTINTL

>CO509836.1 tai58f02.y1 Hydra EST UCI 5 ALP .. same as BP514308.1

IYIAIIIVPLVFFVAVFFKRFYHQFRLLPSPKESLITCHYSYFDVHDHVNTLLNFGKEF 181

KDYGLYTINTL

>DN813094.1 ACAC-aab89g09.g1 Hydra EST UCI 7..= 1097675463974

MFLICLALLILSIGLFFLRYLLKRIFHPLQLLPSPKEQLITGHISHFQGRDHSNTFLGFN 303

EKFKEEGLCTLDTL

>DN603400.1 ACAC-aac10m18.g1 Hydra EST UCI 7..= 1097675463974

same as 1096091465110 DN813094.1 DN137655.1

MFLICLALLILSIGLFFLRYLLKRIFHPLQLLPSPKEQLITGHISHFQGRDHSNTFLGFN 283

EKFKEEGLCTLDTL

>DN137655.1 ACAE-aaa07c04.g1 Hydra EST UCI 5.. ..= 1097675463974

same as 1096091465110 DN813094.1 DN137655.1

LICLALLILSIGLFFLRYLLKRIFHPLQLLPSPKEQLITGHISHFQGRDHSNTFLGFNEK 192

FKEEGLCTLDTL

>CN567598.1 tag12b09.x1 Hydra EST -Kiel 1 Hy..we have this one

LLKRIFHPLRFLPSPKEQLITGHINHFQGRDHSSTYLSFNEKFKEESLCTLDTLH

>CX833403.1 ACAC-aaa40d06.g1 Hydra EST UCI 7..

91% to 1095899139433 exon 2

IIFTVLFW*RTFHPLQLLPSPKEQLITGHNMYFHGRDHTSTYLSFNKQFKK*GLCTQHTLX

VPRYVYLIAPQFITKIFAYGKLFQRPTTLKILAPLIGNSMLGSNYKDHHWQKKLFNGAFT 431

SQQLKNYFPAFLKHTT*LMKHWSYTCDKESGTNLTVLDDLSNLSFNIVGDVGFLGFGYQFTQ

ITSHASNEYTS

>1097675877620 new exon 1 with one possible frameshift or there is another exon

MYMICIAAIVILCFL

VLAVMLKRFYYPLCMLPSPKENLFTAHYRYFYGHDHINAFLNFQNQFKDYGLYTLDLLLG (1)

ATGTATATGATTTGCATAGCAGCCATTGTTATATTATGTTTTCTC

GTCCTTGCTGTTATGTTAAAACGTTTTTATTATCCGCTTTGTATGCTTCCATCACCCAAA

GAAAATTTATTTACAGCTCATTATAGATATTTTTATGGTCATGATCATATCAACGCTTTT

TTAAATTTTCAAAACCAGTTTAAAGACTATGGCTTGTATACATTAGATTTATTACTTGGT

>1095901177607 new exon 1 only 5 aa diffs from 1097675877620

all three of these sequences seem to have a frameshift. So this is probably

evidence for another upstream exon. They almost certainly don’t have a frameshift

MYMICIAAIVILCFL

VLAVMLKRFYYPLCMLPSPKENLFTAHYRYIYGHDHINAFLYFQNQFKEYGLYTLDIFL

ATGTATATGATTTGCATAGCAGCCATTGTTATATTATGTTTTCTC

GTCCTTGCTGTTATGTTAAAACGTTTTTATTATCCGCT

TTGTATGCTTCCATCACCCAAAGAAAATTTATTTACAGCTCATTATAGATATATTTATGG

TCATGATCATATCAACGCTTTTTTATATTTTCAAAACCAGTTTAAAGAATATGGCTTGTA

TACATTAGATATATTTCTTGGT

>DN813094.1 ACAC-aab89g09.g1 Hydra EST UCI 7..= 1097675463974

MFLICLALLILSIGLFFLRYLLKRIFHPLQLLPSPKEQLITGHISHFQGRDHSNTFLGFN 303

EKFKEEGLCTLDTLY (1)

ATGTTTCTGATTTGTCTAGCACTTTTAATTTTATCTATTGGATTATTTTTTTTGCGT

TATTTATTAAAACGTATCTTTCACCCTCTTCAACTTTTACCATCACCAAAAGAACAACTC

ATTACTGGTCATATTAGTCACTTTCAAGGCCGCGACCATTCCAACACCTTTTTGGGTTTC

AACGAAAAGTTTAAAGAAGAAGGTTTATGTACGCTAGATACATTATATGTGCCCAGGTAT

>1097675463974 mate pair to exon 2 1097675525814

also 1096123961892 1097329363407 1097509311387

1096064006710 1097509202292 1096703858851 1097675514139 1096607047135

walked up to 1096526789565 continued on 1097516017620

(1) VPRYVYLIAPEFIKKIFADGKLFQRATSLKVLAPIIGNSMLTSNYEDHHWQRKLFNGAFT 565

SQQLKNYFPAFLTHTDFLMK (0)

(1) AGTGCCCAGGTATGTTTATCTAATTGCTCCAGAATT

TATAAAAAAGATATTTGCAGATGGAAAATTTTTTCAAAGAGCTACTTCATTAAAGGTTTT

GGCACCTATAATTGGAAATAGCATGCTTACTTCAAATTACGAAGACCATCATTGGCAAAG

AAAGTTATTCAATGGAGCATTCACTTCACAACAGCTAAAAAACTACTTTCCTGCATTTTT

AACGCATACTGATTTTCTAATGAAAGTAAGTTTTGATAATATTTAGTTATAGTTTTGTTG

TTTTTATTATAATAATGCAAAACAAATTCTTTTAGCTTGTAAGTACATGGT

>gnl|ti|647058148 1095898198167 I-helix mate pair = 1095898261914

1095898261914 1097206705284 1096761285028 1096761249195 1097206596155

1097675525814 1097675035030 1097329367631 1097206911388 1097206828844

1097460197276

LWSYTSDKESGTNLTVLDDLSNLSFDIIGDVGFGYQFNTINSHSGNEFT

SAFRYLTELQHNASVFSKVLISCFPFLAQFLLLFGKRRKLIQVVHKTLNK (0)

1097664219266 1097664219266 1096123852196 1097206273255

these have 2 aa diffs 1097331817534 1097206329872 1096526011941

(0) LIEKRKKEIDDGISTEEKDIITIVLKDQQQESSKLTNDLIRDNLLLFLIAGHETTSTAMTWCLYMLGT (0)

AGCTTTGGTCTTATACAAGTGATAAAGAAAGTGGGACAA

ACTTAACTGTTTTGGATGATTTGTCTAATCTGTCCTTTGATATAATTGGTGATGTTGGTT

TTGGCTACCAATTTAACACAATCAACTCTCATTCTGGTAATGAATTTACATCAGCTTTTA

GATATTTGACTGAACTGCAACATAATGCTAGTGTGTTCTCAAAAGTTTTGATAAGTTGTT

TTCCGTTTTTGGCGCAATTTTTGTTATTGTTTGGAAAACGTAGAAAACTTATACAAGTTG

TCCATAAAACTTTGAATAAGGT

1096123183594 1097335028467 mate pair 1097335034435 = 100% match to 1095898198167

1097325031454 1097509311387

(0) NLEVQEKLREEIQKNILDKKNITFEEILSLKYLDCVVKETLRLHGPAPILGRRNINATKF 957

GEYEVPANTVLRTH VSSLHMNETIYPDPHSFKPERFMT (1)

AGAACTTAGAAGTTCAAGAAAAACTTAGAGAAGAGATCCAGAAAAATATATTGGATAAAAAAAAT

ATTACTTTTGAAGAAATCTTGAGTTTGAAATACTTAGATTGTGTCGTTAAAGAAACCTTG

CGCTTGCATGGACCAGCACCAATTTTAGGCAGAAGAAACATTAATGCAACAAAATTTGGC

GAATATGAAGTTCCTGCCAACACAGTACTACGAACTCAT

EST = DN137322.1 extends to end of gene = 1096071008743 1097672372091

1097509003243 1097329517617

LLFLIAGHETTSTAMTWCLYMLGT NLEVQEKLREEIQKNILDKKNITFEEILSLKYLDCV 579

VKETLRLHGPAPILGRRNINATKFGEYEVPANTVLRTH

VSSLHMNETIYPDPHSFKPERFMT

1096625230620 1096705372268 1097675934526 1097622179423 1097265071715

1097675205779

GEIPATFYLTFGHGIYNCIGKNFALLEIKTFLVKALLQFEFSVDPEHISYKKFIWLTTITAEPLSIRVKPIAD*

GTTAGCAGTCTACACATGAATGAGACTATTTATCCAGATCCTCATTCGTT

TAAACCTGAAAGGTTTATGACAGGCGAAATACCAGCAACATTCTATCTTACTTTTGGGCA

CGGTATATATAACTGTATTGGAAAGAATTTTGCTTTGCTTGAAATCAAAACATTCTTGGT

CAAAGCGTTGTTGCAATTCGAATTTTCTGTTGACCCTGAACATATCAGTTACAAAAAGTT

TATTTGGTTAACTACGATAACAGCAGAACCATTGTCAATTAGAGTAAAACCTATTGCAGATTGA

>1096703991752 1096123270489 1096526190394 probably same as 1096625230620

GEIPATFYLTFGHGIYNCIGKNFALLEIKTFLVKALLQFEFSVDPEHISYTKFVWLTTXXXXXXIRVNLIAD*

AGGCGAAATACCAGCAACATTCTAT

CTTACTTTTGGGCACGGTATATATAACTGCATTGGAAAGAATTTTGCTTTGCTTGAAATC

AAAACATTCTTGGTCAAAGCGTTGTTGCAATTCGAATTtTCTGTTGACCCTGAACATATCA

GTTACACGAAGTTTGTTTGGTTAACTACGXXXXXXXXXXXXXXXXXXGTCATTAGAGTAAACC

TAaTTGCAGATTaa

>these have 2 aa diffs from 1097675463974, 1097331817534 1097206329872 1096526011941

(0) LIEKRKKEIDDGISTEEKDIITIVLKDQQQESSKLTNDLIRDNLLLFLVAGHETTSNAMTWCLYMLGT (0)

>1096071008743 84% to CN567799 1096602116307 1096123983311 1096124057195

886 (0) NLEVQDKLREEILKNILDVNNISFEEVMSLKYLDCVVKETLRLHGPAPILRRRTMNAIKF 707

706 GEYEVPANTVLQTHISSLHMNETIYADPHLFKPERFMT (1) 593

>gnl|ti|648470985 1095898761545 N-term mate pair = C-term 1095899295538

1097264057439 extends N-term down 1097325864056 joins N and C-terms

681 MFLVYSLLVVIFSYFLIKISWKLWIYSYGLSTVPTPPTIPFFGNCLQLESDSVKFNKQI 854

855 REWSKIYGNVFCVWIGLTPMIYSSSVNFSEAILSSQKVLKKASVYEFLYEWLQTGLLTSTGNK

WKLRRRLLTPSFHFSILNNFLKIFEEQGACLVDKLRIYAKSGGNFDIQVPIGLATLDIICETSM

GVKVNAQSHPDSEYAKAIGILSEEIPKRIKYPWLWPDIIYKHLACGKRYYKALDVAHKLSLDVI

KERVKTLIQNKSEVTSNKNKK

ATGTTTTTGGTGTACAGTCTATTGGTTGTTATTTTTTCATACT

TTTTAATTAAAATATCTTGGAAACTTTGGATTTATTCTTATGGTCTCTCGACTGTTCCAA

CACCTCCAACCATACCATTTTTTGGCAATTGTCTTCAGCTTGAAAGTGATTCTGTAAAGT

TTAACAAACAAATACGCGAGTGGAGCAAAATATACGGAAATGTTTTCTGCGTTTGGATAG

GCCTTACGCCAATGATATACTCATCTTCTGTAAATTTCTCGGAAGCAATCTTAAGCAGTC

AAAAAGTCCTCAAAAAAGCATCTGTTTATGAATTTTTGTATGAATGGCTTCAAACCGGG

TTACTGACAAGCACAGGAAATAAG

TGGAAACTGCGTCGTCGACTTCTTACACCAAGCTTTCATTTTTCTATACTCAATAATT

TTTTAAAAATTTTTGAAGAGCAAGGAGCTTGTTTAGTTGATAAATTACGTATTTATGCCA

AAAGTGGTGGAAATTTCGATATCCAGGTACCTATTGGATTAGCAACTTTAGATATAATAT

GCGAGACATCAATG

GGAGTAAAAGTAAATGCACAGAGTCACCCAGACTCAGA

GTATGCTAAAGCCATCGGTATATTAAGTGAAGAAATACCAAAAAGAATTAAGTACCCATG

GTTATGGCCAGATATTATTTATAAACATCTTGCTTGTGGAAAAAGATATTATAAAGCTCT

AGATGTTGCTCATAAATTATCTCTTGATGTAATAAAAGAAAGAGTTAAAACACTTATTCA

AAATAAAAGCGAGGTTACATCAAATAAAAACAAAAAA

GAATCAGGCTCTGAAAAAAAAAA

ATTTTTTTTAGACTTATTGTTAGATATGCATAAAAAAGGTGAAATTGATACTGAAGGGAT

TCAAGAAGAGGTTGATACTTTTATGTTTGAAGGTCATGATAGCACTTCATCAGCATTAAG

CTGGATGCTGTGGTTGCTAGGAAGATATCCACAAGTTCAACAGAAACTGCATTCAGAAAT

TGATGAAGTGgAA

1095899295538 1096703530556 1096705948493 1096526527227 1096625218937

1097191001062

ESGSEKKKFFLDLLLDMHKKGEIDTEGIQEEVDTFMFEGHDSTSSALSWMLWLLGRYPQVQQKLHSEIDEVE

LTGGSLYEKVRNFKYLENVVKESMRIHPPVPLIGRHIEEDMVIDGQFVPKSSEIVLLVMM

MQSSPEYWKDPYDFIPERFEQEDFVKRNPYIYIPFSAGPRNCIGQKFAMIEEKMLLYIIM

KNFYVQSIQNENEILLALNIIHKSSNGIIMKFTER*

TTAACTGGAGGTTCACTTTATGAAAAAGTAAGAAACT

TTAAATATCTTGAAAACGTTGTAAAAGAAAGTATGCGAATTCACCCACCTGTTCCTTTAA

TTGGCAGGCATATTGAAGAAGACATGGTAATTGATGGTCAGTTTGTTCCTAAAAGTTCTG

AAATTGTTTTACTTGTAATGATGATGCAATCAAGTCCTGAATACTGGAAAGATCCATATG

ATTTCATACCTGAAAGGTTTGAACAAGAAGATTTTGTTAAGCGCAATCCATATATCTATA

TTCCATTTTCAGCAGGTCCAAGAAACTGTATTGGTCAGAAGTTCGCAATGATTGAAGAGA

AAATGCTGTTATATATCATAATGAAAAACTTTTACGTCCAATCCATCCAGAATGAAAATG

AAATACTTCTTGCTCTAAATATTATACATAAATCGAGTAATGGTATCATAATGAAATTCA

CTGAAAGATGA

>1096083942127 1097329109827 clearly best match to 4V sequences

MAFILLIFFLLLITLFLIWIYWVRSYNLNFVPSPLRFPLFGCALFLKSESH

ELFKQVRWFFSEFGSAFCLWIGPKPVLMTGNIDHIQTVLKSQKIITKSSSYTFLNE

WLGTGLLTSTGAKWKSRRKVLTKAFHFSIINSYVDSFYQNSVSLSNHLENHSGVPINIQA

LMSLFTLDIICETAMGFKLNSMKNLNCDYVNAVEEVKILLIERQKSPWLWNKFVYKLFSS

GKKFYTQLQVLKSFTKKIVNKRIKNYSLSSNGCKSFLDLLIDAYNQGKIDLEGIYEEVDT

FMFAGHDTTAAALSYIFLMLGTHPKVQKKLHEEIDTNVNINSYENLSEKIRKMEYLDCVI

KESLRLHPPVSVFGRILEDDTIFSNHLVGKGADIVLCPETLHTDPLYWENHRSFIPERFS

NVEFAFCQPYLYIPFSAGPRNCIGQKFALMEIKIAIFVVMSKFIVTAVEQCLSPM

ATFIQRYENGVLMLFEDEKRFLYML*

>1097329374310 no introns very similar to 1095899295538 seq

1096608398403 1096761840588 1097460256370

67% to 1095958068757 88% to 1095898761545

MFIAYSLLVVVSLYFVIKLFWK

FWIYSYGLSTVPTPPTIPFFGNSLQLESDSVKFNKQLCEWSKIYGNVFCVWVGLR

PTIFSSSVNFSEAILSSQEVLKKASIYEFLHDWLKTGLLTSTGNK

WKLRRRLLTPSFHFSILNNFLKIFEEQGACLVDKLRTYAKSGENFDIQVPIGLATLDIICETSMGVKVNAQSHP

DSAYVKAINILSEEIPRRFKYPWLWPDIIYKHLACGKRYYKALDVAHKLSLDVINERIETLFQNE

NNVTTNKNKEVSSEKKKFFLDLLLDIHKKGEIDTEGIQEEVDTFMFEGHDTTSSALSWIL

WLLGRYPQVQQKLHSEIDEVELTGGSLYEKVRNFKYLENIIKESLRIHPPVPLIGRHIEK

DMVIDGQFIPKKSEIGVLVMMMHSSPEYWKDPYDFIPERFEQEDFVKRNPYIYIPFSAGPRNCIG

QKFAMIEEKMLLYSIMKNFYVQSMQNENEILPSLDLIRKSVNGIILKLTER*

ATGTTTATTGCGTACAGTTTGTTGGTTGTAGTTTCTTTATACTTTGTAATTAAATTATTTTGGAAG

TTTTGGATTTATTCTTATGGTCTCTCGACTGTTCCAACACCTC

CAACCATACCATTTTTTGGGAATTCTCTTCAACTTGAAAGTGATTCTGTTAAGTTTAATA

AACAACTATGCGAGTGGAGCAAAATATACGGAAATGTGTTCTGTGTTTGGGTAGGCCTTA

GGCCAACTATTTTCTCATCTTCTGTAAATTTCTCGGAAGCAATTTTAAGCAGTCAAGAAG

TCCTTAAAAAAGCATCAATTTATGAATTTTTGCATGACTGGCTTAAAACTGGATTACTAA

CAAGCACAGGAAATAAGTGGAAACTGCGTCGTCGACTC

CTTACACCAAGCTTTCATTTTTCTATACTCAATAATTTTTTAAAAATT

TTTGAAGAGCAAGGAGCTTGTTTAGTTGATAAACTACGTACTTATGCCAAAA

GTGGTGAAAATTTTGATATCCAGGTACCTATTGGATTAGCAACTTTAGATATAATATGTG

AGACATCAATGGGAGTAAAAGTAAATGCACAGAGTCACCCAGATTCAGCGTATGTTAAAG

CCATTAATATTTTAAGTGAGGAAATACCAAGGAGATTTAAATACCCATGGTTGTGGCCAG

ATATTATTTATAAACATCTTGCTTGTGGAAAGAGATATTATAAAGCACTAGATGTTGCTC

ACAAATTGTCTCTAGATGTAATAAATGAAAGAATTGAAACACTTTTTCAAAATGAAAACA

ATGTTACCACAAATAAGAACAAAGAAGTTAGCTCAGAAAAAAAAAAGTTTTTTTTAGACC

TACTGTTAGATATACATAAAAAAGGTGAAATTGATACTGAAGGGATTCAAGAAGAGGTTG

ATACTTTTATGTTTGAAGGTCATGATACCACCTCATCAGCATTAAGCTGGATACTTTGGT

TGCTAGGAAGATATCCACAAGTTCAACAGAAACTGCATTCAGAAATTGATGAAGTTGAAT

TAACCGGAGGTTCACTTTATGAAAAAGTAAGAAACTTTAAATATCTAGAAAACATCATAA

AAGAAAGCCTGCGAATTCATCCGCCTGTTCCTTTAATTGGCAGACATATTGAAAAAGATA

TGGTAATTGATGGTCAGTTTATTCCTAAAAAATCTGAAATTGGTGTTCTTGTCATGATGA

TGCATTCAAGTCCTGAATATTGGAAAGATCCATATGATTTCATTCCTGAAAGGTTTGAAC

AAGAAGATTTTGTTAAGCGCAATCCCTATATCTATATTCC

ATTTTCTGCAGGTCCGAGAAATTGTATTGGTCAGAAGTTCGCAATGATTGAAGAGAAAAT

GTTGTTATATAGCATAATGAAAAACTTTTACGTCCAATCCATGCAGAATGAAAATGAAAT

ACTTCCTTCTCTAGATCTTATACGTAAGTCGGTTAATGGTATCATATTAAAACTTACTGA

ACGATAA

>1095964281471 1097672357643 1097675038710 1096526281478 1097675573844

MFSNIKMIYTLCIIICGFYFLIKILWMCWKYSYGLTSIATPPNTPFLGTSFYFLSDS

RKSYFQLCNYTKQFGNVFCIWLGPKPMIVSSSVKFLKAVLSSEKITTKGFSYDWIHDWLK

TGLLTSSGPKWKARRKLL

TSSFHFSVFNRLKIIIEEQACILVDKISFAADNKKVVDVQTLIGLATLDVICETIMGVKINAQ 780

SYPDSEYVKAISVLHKEIVNRMKFPWLWFDVIYKLLPCGKRFYKALDVAHKFTFDIINKR 600

MEISVNESYIDTPLEEKSYFLDLLLNIHKKKEIDMEGIQEEVDTF

IFAGHDTISVALSWTLWLLGKYSEIQRKLHKSIDEIELNGGSLFEKVRNFKYLENII

KESMRIHPPVPMYGRTVEENMTIDGQFVPKGAQIILLVLMLHSDPNIWENPKEFIPERFE

TDDWKIKNSYSYLPFSAGSRNCLGQKFAMIEAKMLLYSIM

KKFSLKSMQDENEVYGTVDILHKSINGINILFTRR*

>gnl|ti|648478468 1095898788708 N-term EST = CV564880.1

1097672125473 1097509103730 1096123847153 1097664004740 1097329293298

1096092407854 1097325278081 1097675392269 1097672158546 1097206129107

1095899351259

76% to 1097329374310 similar to 4V5

MFLTFMFLFLIYFLIKVFWKLWIYSYGLSTVSTPPTLPLFGNCLQIKSDPVKASKQL

FEWSRVYGKVFCVWVGIRPTIFSSSVNFSEAILSSQKIIQKGFVYNFLHEWLKTGLLTST

GNKWKLRLRLLTPSFHFSILNNFLKIFEEQGNCLIDKFRVLAQNGKYFDIQVPIGLATLD

IICETSMGVKINAQYQPDSEYVTAINILSEEIVRRFKYPWLWPNIFYKHFSCGKRYFKAL

DIAHKLSLNVIHERIQTSLQNESENVLINKLDNKSVLNNEEELGVRKKRFFLDLLLDMHK

KGEIDVDGIQEEVDTFMFEGHDTTSS

AMCWTLWLLGRYPQIQQKLHAEVDEVELTSGSLYEKVRNFKYLE

NVLKESLRLHPPVPLISRYIEEDMMIDGQFIPKKSEIAILVMMIHLNPEYWKDPHSFIPE

RFDQDDFVKRNPYTYIPFSAGPRNCIGQKFAMIEEKMLLYNIMKHFYVESMQNENEILRT

QDLISKSANGIMMKFYER*

ATGTTTTTAACTTTTATGTTTTTGTTTCTTATTTATTTTCTAATTA

AAGTATTTTGGAAGCTTTGGATTTATTCTTATGGCCTGTCAACTGTTTCTACACCTCCCA

CATTACCATTATTTGGCAATTGTCTTCAAATCAAAAGTGATCCTGTAAAAGCCAGCAAAC

AACTATTCGAGTGGAGCAGAGTATACGGAAAAGTGTTTTGTGTTTGGGTTGGCATTCGGC

CAACTATATTCTCATCTTCTGTTAATTTTTCCGAAGCAATTTTAAGCAGTCAAAAAATAA

TTCAAAAAGGATTTGTGTACAATTTTTTGCATGAATGGCTTAAAACTGGTCTACTAACAA

GTACGGGAAATAAGTGGAAATTGCGTCTTCGACTTCTAACGCCAAGCTTTCATTTTTCTA

TACTCAATAACTTTTTAAAAATTTTTGAAGAGCAAGGAAATTGTTTAATTGATAAATTTC

GCGTTCTTGCCCAAAATGGAAAATATTTTGATATTCAGGTGCCTATTGGGTTAGCTACAT

TAGATATAATATGCGAGACGTCAATGGGAGTGAAAATAAACGCGCAGTATCAGCCAGATT

CCGAATATGTTACTGCCATTAACATCTTAAGTGAGGAAATAGTTAGACGGTTTAAGTACC

CGTGGTTGTGGCCAAATATTTTTTATAAGCATTTTTCTTGTGGAAAACGGTACTTTAAAG

CATTAGACATTGCTCATAAACTGTCTCTTAATGTAATTCATGAAAGAATTCAAACTAGTT

TACAAAACGAAAGTGAGAATGTGTTAATCAATAAACTTGACAATAAGAGCGTGTTGAACA

ATGAAGAGGAACTCGGTGTACGTAAAAAGAGGTTTTTCTTAGATTTATTGTTAGACATGC

ATAAAaAAGGTGAAATT

GATGTTGATGGGATTCAAGAGGAGGTGGATACATTTATGTTTGAAGGTCACGACACCACC

TCATCAGCAATGTGTTGGACATTATGGTTGCTGGGAAGATATCCACAAATTCAACAGAAA

CTGCATGCTGAAGTTGATGAAGTTGAACTAACTTCGGGTTCACTATATGAAAAAGTACGA

AACTTTAAATATCTTGAAAATGTTTTAAAAGAAAGCCTGAGACTTCATCCACCAGTTCCC

TTAATCAGTAGGTATATTGAAGAAGATATGATGATTGATGGTCAGTTTATTCCTAAAAAA

TCTGAAATCGCTATTCTTGTGATGATGATACACTTAAATCCTGAGTATTGGAAAGATCCT

CACAGCTTTATACCTGAAAGATTTGATCAAGATGATTTTGTAAAGCGTAATCCATACACT

TACATTCCATTCTCCGCTGGCCCTAGAAATTGCATTGGTCAAAAGTTTGCAATGATAGAA

GAAAAAATGCTGTTATATAACATAATGAAACATTTTTATGTAGAATCCATGCAGAATGAA

AATGAAATTTTAAGAACTCAAGATCTTATAAGTAAATCAGCTAATGGTATCATGATGAAGTTCTATGAAAGATGA

>Combined CN627429 CN775805 27% to 4T5 [gene 6]

GIAVLIFLCFSLFFANILKRFYHPLRKLPSPKENFFTAHYGYFNGYDQINAVINFGKQFK

ERGLYTLDTLNGFRFVNLLMPEFIKTVFSDGNSFQRSTATKVIFPLVGNGIFVSNYEDHH

WQRKVLNEAFTLQQLKNYFPAFTVHIDLLMKLWSYSCDK

DNGTNIIVLDDLSNLSFDIIGDVGFGYQFNTITSHSGNEFTKALQSYCQLRFQLNAVHKALLA

FFPFLMHLSFMYGKRKRAEQVICNTLNM

LINKRKKEIDHRIAADQKDFLTVVLK

DQQKEGNKMTNDLIKNNLMTPLIAGHKTTSTDMPWCFNVLAPNPSATKHMQRNRKKEYISTTK

>EST DN812371.1 joins with CN627429 CN775805 and 1095901729505 1097325001902

CN776982 and CN770283 1097206896815 1096110026952 1096110107596

MYTIGIAVLIFLCFSLFFANILKRFYHPLRKLPSPKENFFTAHYGYFNGYDQINAVINFGKQFKERGLYTLDTLN

GFRFVNLLMPEFIKTVFSDGNSFQRSTATKVIFPLVGNGIFVSNYEDHH

WQRKVLNEAFTLQQLKNYFPAFTVHIDLLMK

LWSYSCDKDNGTNIIVLDDLSNLSFDIIGDVGFGYQFNTITSHSGNEFTKALQSYCQL

RFQLNAVHKALLAFFPFLMHLSFMYGKRKRAEQVICNTLNM

LINKRKKEIEDGIAADQKDFLTVVLKDQQKEGSKMTNDLIKDNLMTLLIAGHETTSTAMQWCLYMLGT (0)

NLGVQNKLREEIKKNVFDIKSVSYEEVLSIKYLDCVVKETLRMHTPVAFIGRINKNQTKFGDFDV

PAGSFLRIPIDSAHMNESVYHDPYSFRPERFLTGEIPPLSFLTFGQGIYNCIGKNFALLE

IKTFLVKALLQFEFSVDLKHLNYKKLISITNKTVEPLWIRVKPI*

EST matches 1097325001902 1097265052814 for exon 1

1 tcggcatagc agtattaatt tttttgtgtt tttcactgtt ttttgctaat attttaaaac

61 gtttttatca tccgcttcgt aagttgccat cacctaaaga aaatttcttt actgctcatt

121 atggctactt taatggctat gatcaaataa atgctgtaat aaattttgga aaacagttta

181 aagagcgtgg cttgtataca ttagatacat taaat ggatt tagatttgtt aatcttttaa

241 tgccagaatt tattaaaaca gtgttttctg atggaaactc attccaaaga tcgaccgcta

301 caaaagttat atttcctcta gttggaaatg gtatttttgt gtcaaattat gaagatcatc

361 attggcaaag aaaagtgtta aatgaagctt ttactttaca acagctaaaa aattattttc

421 cagcttttac agtgcacatt gatttgctaa tgaaactttg gtcatattca tgtgacaagg

481 ataatggtac taacataatt gttttggatg acttatctaa tttatcattt gatataattg

541 gggatgttgg ttttggctat ca

>1096526100337 74% to CN776982

1096526100337 1097206278072 1096123494736

(0) NLDVQNKLREEIKKNVFDIKSILREEVLSIKYLDCVVKETLRMHPPASFISRKNKTETKL 308

GDYDIPAGTFLRISINNVHMNESVYPDPYLFKPERFMT (1)

1095898850029 same as 1096520314506 = mate pair match to 1096526207508

1097206730806

DEIPPSSFLSFGQGIYNCIGKNFALLEIKTFLVKALLHFEVSVDPSHVNYTKQILLTLNTVEPIWIRVKSIEE*

AGAATTTAGATGTTCAAAACAAACTAAGAGAAGAAATAAAGAAAAA

TGTCTTTGATATAAAAAGTATTTTACGGGAAGAAGTTTTAAGCATCAAGTACTTGGATTG

TGTAGTTAAAGAGACATTACGCATGCATCCACCTGCGTCATTTATATCTCGAAAAAACAA

AACTGAAACAAAGTTGGGTGATTATGATATACCTGCTGGCACGTTTTTAAGAATTTCAAT

TAACAACGTACATATGAATGAGTCTGTTTATCCTGATCCTTATTTATTTAAGCCGGAACG

ATTTATGACAGGT

AGATGAAATACCACCATCGTCTTTTCTCTCATTTGGGCAAGGTATTTATAATTGTATTGGAAAGAAT

TTTGCTTTGCTTGAAATTAAAACGTTTTTGGTTAAAGCATTATTACATTTTGAAGTTTCT

GTCGACCCAAGTCATGTGAATTATACAAAACAGATTTTGTTAACTTTAAATACCGTTGAA

CCCATTTGGATAAGAGTGAAATCTATTGAAGAATAA

>1097696222067 new exon 6 1097375001145 1097672638278

1096041191032 1097678083218

GEIQPYSYLTFGQGIFNCIGKNFALLEIKTFLVKALLQFEFSVDLEHMNYIKKIFISTKTVEPLWIRVKPI*

AGGTGAAATACAACCAT

ATTCCTACCTCACATTTGGGCAAGGTATTTTTAATTGTATTGGAAAGAATTTTGCTTTGC

TTGAAATTAAAACATTCTTGGTCAAAGCGTTGTTGCAATTCGAATTTTCTGTTGACCTTG

AGCATATGAATTATATAAAGAAAATTTTCATTTCTACTAAAACTGTTGAACCGTTATGGA

TAAGAGTGAAACCTATATAA

>1097331770349 new exon six with stop, no other exact matches

DEIPSSSYLTFGYGIYNCIGKNFALLEIKTFLIKAL*QFEFLVDPEQLSYKKQISIST 330

KTAEPLWIRVKSI*

AGATGAAATACCATCTTCATCCTAC

CTTACATTTGGGTATGGTATTTATAACTGTATTGGAAAGAATTTTGCTTTGCTTGAAATT

AAAACATTTTTGATTAAAGCGTTGTAACAATTTGAGTTTTTGGTTGACCCTGAGCAATTA

AGTTATAAAAAGCAGATTTCAATTTCTACTAAAACAGCTGAACCGTTATGGATAAGAGTA

AAGTCTATATAA

>1097329444796 new exon 6 no other exact matches, most like CN567799

GEIPASFYLPFGHGVYNCIGKNFALLEIKTFLVKALLQFEFSVDPKNINYTKVIWLTTRTVEPLLIRVKPLQPV

AGGCGAAATACCAGCATCGTTCTATCTTCCTT

TTGGACACGGTGTTTATAACTGCATTGGAAAGAATTTTGCTTTGCTTGAAATTAAAACAT

TTTTGGTCAAAGCGTTGTTGCAATTCGAATTTTCTGTCGATCCTAAGAATATAAATTATA

CAAAGGTTATTTGGTTAACTACGAGAACAGTTGAACCATTGCTTATAAGAGTAAAGCCAT

TACAGCCCGTAC

>1097325113147 1097690001285 1097942838551

GEIPATFYLPFGHGVYNCIGKNFALLEIKTFLVKALLQFEFSVDPKHANYTKVIWLTAKT 290

TEPLSIRVKPIVD*

AGGCGAAATAC

CAGCAACATTCTATCTTCCTTTTGGGCATGGTGTTTATAACTGTATTGGAAAGAATTTTG

CTTTGCTTGAAATCAAAACATTTTTGGTCAAAGCGTTGTTGCAATTCGAATTTTCTGTTG

ACCCTAAGCATGCAAATTATACAAAGGTTATTTGGCTAACTGCAAAAACAACTGAACCAT

TGTCAATCAGAGTAAAGCCTATTGTAGATTGA

>1097206250175 1095899118096

GEVPPFSFLTFGRSNYNCIGKNFVLLDIKAFLVKALLQFKFSVDP 360

MHLNYKKPISITNKAVDPLWIRVKTI*

AGGTGAAGTACCGCCATTTTCCTTTCTAACAT

TTGGGCGAAGTAATTATAATTGTATTGGAAAGAATTTTGTTCTGCTTGACATCAAAGCAT

TCTTGGTCAAAGCGTTATTGCAGTTTAAATTTTCAGTAGACCCTATGCATTTGAATTATA

AGAAGCCGATTTCTATTACTAATAAAGCCGTTGATCCCTTATGGATTAGAGTAAAGACTA

TATAA

>1096123749751 boundary is not right, no other exact matches

GETPASLYLPFGHGVYKVIGKNFSLLEIKTLSVKALLQLEKVVDPKNINYSKVIWLTSRT 211

VEPLFIRVKLIVD*

GGTGAAACACCAGCATCGCTTTATCT

TCCTTTTGGACACGGTGTTTATAAAGTCATTGGAAAGAATTTTTCTTTGCTTGAAATTAA

AACATTGTCGGTCAAAGCATTGTTGCAATTAGAAAAGGTTGTCGATCCTAAGAATATAAA

TTATTCAAAGGTTATTTGGTTAACTTCGAGAACAGTTGAACCATTGTTTATAAGAGTAAA

GCTTATTGTAGATTAA

>gnl|ti|654999901 1095901768752 87% to 1095901729505

1095901905311 mate pair links to 1095901795880 exon 5

1097331953492 1097664070304 1096761841205 1097675516783 1096602049536

1096761821875

(0) LINKRKKEIEDGIETGEKDFLTIVLKDQQKEGSKMTNDLIRNNLVTLLIAGHETTSVAMQWCLYILGT (0)

AGCTTATCAACAAACGTAAAAAAGAAATAGAAGATGGAATAGAAACTGGTGAAAAAGATTTTTTAACA

ATTGTTTTAAAAGATCAACAAAAAGAGGGCAGCAAGATGACAAATGATTTGATTAGAAAT

AATCTAGTAACACTTTTAATTGCTGGTCATGAAACAACTTCTGTAGCAATGCAATGGTGC

TTATACATTCTTGGCACAGT

1095901795880 1097491021716 1096123686039 1097672412446

(0) NSDVQNKLREDIKKNVFDIKSITCEEVLSIKYLDCVVKEVLRLHPPVSFIGRINTR

QTNFGEYNVPAGSYLRVPINSAHMNESVYPDPYSFKPERFLT (1)

AGAATTCAGATGTTCAAAACAAGCTACGAGAAGACATAAAGAAAAATGTCTTTGATATAA

AAAGTATTACGTGTGAAGAAGTTTTAAGTATTAAGTATTTAGATTGTGTAGTTAAAGAAG

TGTTGCGCTTGCATCCGCCTGTATCATTTATAGGTAGAATCAACACTAGACAAACAAACT

TTGGTGAATATAATGTACCTGCTGGCTCTTATCTACGAGT

>1097206350025 1097675534489 1096602217388 all with frameshift

(0) LIDKRKKEIEDGIATDEKDLLTIALKDQQKENSKMTX

NLIRDNLMTFLIAAHETTSTGMQWCLYMLGT (0)

AGCTTATCGACAAACG

AAAAAAAGAAATAGAAGACGGAATAGCAACTGATGAAAAAGATTTATTAACAATCGCTTT

AAAAGATCAGCAAAAAGAAAACAGCAAGATGACCTTNAATTTAATTAGAGATAATCTAATG

ACATTTTTAATTGCTGCTCATGAAACAACTTCTACGGGAATGCAATGGTGTTTGTATATG

CTTGGCACAGT

>1097331459342 framshift and short 2 aa (pseudogene?)

LIDKRKKEIEDGIATDEKDLLTIALKDQQKENSKMT

NLIRDNLMTFLIAAHETTSTGMQWCLYML

AGCTTATCGACAAACGAAAAAAAGAAATAGAAGACGGAATAGCAACTGATGAA

AAAGATTTATTAACAATCGCTTTAAAAGATCAGCAAAAAGAAAACAGCAAGATGACCTTA

ATTTAATTAGAGATAATCTAATGACATTTTTAATTGCTGCTCATGAAACAACTTCTACGG

GAATGCAATGGTGTTTGTATATGCTG

>1097263640455 new exon 5

(0) NLDVQEKLREGIKKNVSDIKNISYEEVLSNKYLDCVVKEALRIHPPRS

AGAATTTAGACGTTCAAGAAAAACTAAGAGAAGGGATAAAGAAGAATGTA

TCTGATATAAAGAATATTTCATATGAAGAGGTTTTAAGTAACAAGTACTTAGATTGTGTA

GTTAAAGAAGCATTGCGCATCCATCCACCGCGCTCCAGCTA

>1096526374787 no 100% matches to this seq, best match is 1095901795880

but intron boundaries do not match

this may be a poor quality sequence or pseudogene

(1)EKVINIKYLDCVVKEVLRLHPPVLFIGRINTRQTNLGKYIETAGSNQRVPINNAHMNESVYPDPYSFMPKRLLT (1)

AGAAAAAGTTATAAATATTAAGTATTTAGATTGTGTAGTTAAAGAAGTGTTGCGCTTGCA

TCCGCCTGTATTATTTATAGGTAGAATCAACACTAGACAAACAAACTTAGGTAAATATAT

AGAAACTGCTGGCTCTAATCAACGAGTTCCTATTAACAATGCTCATATGAATGAGTCTGT

TTATCCTGATCCTTATTCATTTATGCCAAAGAGGTTGCTGACAGGT

>CN567598.1 tag12b09.x1 Hydra EST -Kiel 1 Hy..

LLKRIFHPLRFLPSPKEQLITGHINHFQGRDHSSTYLSFNEKFKEESLCTLDTLH

>Combined N and C-terms CN567799 CN567598 tag12b09.x1 [gene 4] N-term

N-term has an extension

1096761916754 probably 1095964418219 (poor quality seq)

RVRFLLRYLLKRIFHPLRFLPSPKEQLITGHINHFQGRDHSSTYLSFNEKFKEESLCTLDTLH (1)

VPRFVYLIAPEFIKKIFADGKLFQRSKSIRTLAPLIGNSMVGSNYEHHHWQRKLFNG

AFTSQQLKNYFPAFLKHTNLLMK

(0) LWSYTCDKESGTNLTVLDDLSNLSF

CN567598 part of three exons

1 cacgcgtccg atttttactg cgttatctat taaagcgcat ctttcaccct cttcgatttt

61 taccatcacc aaaagaacaa ctcattactg gtcatattaa tcactttcaa ggccgcgacc

121 attctagcac ctatttgagt ttcaacgaaa agtttaaaga agaaagttta tgcacgctag

181 atacattaca t

gtgcccagg tttgtttatc taattgctcc agagtttatt aaaaagatat

241 ttgcagatgg aaaacttttt caaaggtcaa aatcaataag aactttggcc cctttaattg

301 gaaacagcat ggttggttca aattacgaac accatcattg gcaaagaaag ttattcaatg

361 gagctttcac ttcacaacaa ctgaaaaatt attttccagc atttttaaaa catactaatt

421 tgcttatgaa g

ctttggtca tatacatgtg ataaagaaag tgggacaaat ttaactgttt

481 tggatgattt gtctaatctg tcatttg

DIVGDVGFGYHFNTITSHSGNEVTKAFQKY

CQLRHSLHPFYKALFAYFPFLMRLSFMFGKHKKAEQVISYTXXX (0)

AGCTTTGGTCATATACATGCGATAAAGAAAGTGGTACCAACATAATTGTTTT

GGATGATTTGTCTAATCTATCATTTGATATAGTTGGTGATGTTGGTTTCGGCTATCATTT

TAACACCATAACTTCTCATTCCGGTAATGAAGTTACAAAAGCCTTCCAAAAGTATTGTCA

ACTACGACATAGCTTGCATCCCTTTTATAAAGCTTTATTTGCTTATTTTCCATTTTTAAT

GCGTCTATCATTCATGTTTGGAAAACATAAAAAAGCTGAGCAAGTTATAAGTTATACT

>1096081231152 new exon 3

IWSYTCDKENGTKIIVLDDLSNLSLDIIGDVGYGYQFNTLTSHSGNEFTKAFQSYCQLQY 135

NIKPIYKALSAFFPFLMGLSIMFGKRKKTEEILRNNLNM

AGATTTGGTCATATACATGTGATAAAGAAAATGGTACCAAAATAATTGTTTT

AGATGACTTGTCTAATTTATCACTTGATATAATTGGTGATGTTGGTTATGGCTATCAATT

TAACACCTTAACTTCTCATTCTGGTAATGAATTTACAAAGGCTTTTCAAAGTTATTGTCA

ACTACAATATAACATAAAGCCAATCTATAAAGCTCTATCAGCTTTTTTTCCTTTCCTAAT

GGGGCTGTCAATCATGTTTGGAAAACGAAAGAAAACAGAGGAAATTTTACGTAATAATCT

AAACATGGT

>1095898814465 new exon 2 mate pair to 1095899110069

1095993318769 1095963238168 1096704141526 1095899349182 1097664110168

1097622218011

(1) VPRYVYLIAPEFIKKIFADGKLFQRTTSIRIMAPSIGNSMLSSNYEDHHWQRKLFNGAFT 471

SQQLKNYFPSFLTHTNLLMK (0)

AGTGCCCAGGTATGTTTATCTAATTGCTCCAGAATTTATTAAAAAAATATTTGCTGATGGCAAACTTTTT

CAAAGAACTACTTCAATTAGAATTATGGCACCTTCAATTGGAAACAGCATGCTTAGTTCA

AATTACGAAGACCATCATTGGCAAAGAAAATTATTCAATGGAGCATTCACTTCACAACAG

CTAAAAAACTATTTTCCTTCATTTTTAACGCATACTAATTTACTGATGAAAGT

1097567103129 1097675494277 1095899110069

(0) IWSYTCDKESGTNLTVLDDLSNLSFDIIGDVGFGYQFNTITSHSRNEFTSAIRYLAEIQL 657

NASVFLKVLISYFPFLIQLLVMFGKRRKFIQIVRKTLNK (0)

AGATTTGGTCTTATACATGTGATAAAGAAAGTGGGACAAACT

TAACTGTTTTGGATGATTTGTCTAATCTGTCATTTGATATAATCGGTGATGTTGGTTTTG

GTTACCAATTTAACACAATTACATCTCATTCTCGTAATGAATTTACTTCAGCTATTCGGT

ATTTGGCTGAAATTCAACTCAATGCTAGTGTGTTCTTAAAAGTTTTAATAAGTTATTTTC

CATTTTTAATTCAATTGTTGGTAATGTTTGGGAAGCGTAGAAAATTTATACAGATTGTCC

GTAAAACATTGAACAAGGT

39% to 3A27 trout aa 307-472 58% to CN770283 [gene 4]

683 FFIAGYETISTTLTLCLYMLAINLEVQEKLREEIQKNKLDVNNISFEEVTSLKYLDCVVK 504

503 ETLRLHGLAPVLGRETINAIKFGEYEIPANTVLQTHVSNLHMNETIYRDPHSFKPERFMT 324

323 GEIPASFYLPFGHGVYNCIGKNFALLEIKTFLVKALLQFKFSIDPMHINYTK 168

167 IIWLTMRTVEPLLIRVKPIAE* 102

TTTTTCATAGCTGGTTATGAAACAATTTCTACTACTTTGACTTTGTGTTTATATATGCTA

GCCATTAACTTAGAGGTTCAAGAGAAACTTAGAGAAGAGATTCAGAAAAATAAATTGGAT

GTAAATAATATTTCTTTTGAAGAAGTTACGAGTTTAAAATATTTGGATTGTGTCGTTAAA

GAAACCTTGCGCTTGCATGGACTTGCACCAGTTTTAGGCAGAGAGACCATTAATGCAATA

AAATTTGGCGAATATGAAATTCCTGCAAACACAGTACTTCAAACTCATGTTAGCAATCTA

CACATGAATGAGACTATTTATCGAGATCCTCATTCATTTAAACCTGAAAGGTTTATGACA

GGGGAAATACCAGCATCATTCTATCTTCCTTTTGGGCACGGTGTTTATAACTGTATTGGA

AAGAACTTTGCTTTGCTTGAAATTAAAACATTCTTGGTCAAAGCGTTGTTGCAATTCAAA

TTTTCTATTGACCCTATGCATATAAATTATACAAAGATTATTTGGTTAACTATGAGAACA

GTTGAACCATTGCTAATTAGAGTAAAACCTATTGCAGAATAA

>gnl|ti|648014530 1095896049543 41% to CYP21

LKYLDCVVKETLRLHGXXXXXXXXXXXXX

KFGEYEVPANTILRTHVSSIHMNETIYPDPHSFKHERFMTG

GTTTAAAATATTTGGATTGTGTCGTAAAG

GAAACCTTGCGCTTACATGGA

AAATTTGGTGAATATGAAGTCCCTGCAAATACAATCC

TACGAACTCATGTTAGCAGTATACACATGAATGAAACTATTTATCCAGATCCTCATTCAT

TTAAACATGAAAGGTTTATGACAGGT

>1096082202706 probably the same as 1095896049543 which has errors

1095994179331

(0) NIEVQEKLREDIQKNILDVNNISFEEVMSLKYLDCVVKETLRLHGPAPLLGRRTISATKF

GEYEVPANTILRTHVSSIHMNETIYPDPHSFKPERFMT (1)

AGAACATAGAAGTTCAAGAGAAACTTAGAGAAGATATCCAGAA

AAACATATTGGATGTAAATAATATTTCTTTTGAGGAAGTTATGAGTTTAAAATATTTGGA

TTGTGTCGTTAAAGAAACCTTGCGCTTACATGGACCTGCACCACTTTTAGGCAGAAGGAC

CATTAGTGCAACAAAATTTGGTGAATATGAAGTTCCTGCAAATACAATCCTACGAACTCA

TGTTAGCAGTATACACATGAATGAAACTATTTATCCAGATCCTCATTCATTTAAACCTGA

AAGGTTTATGACAGGT

>1096526207508 with frameshifts probably same seq as 1096526100337

mate pair = 1096520314506 same as 1095898850029

(0) NLDVQNKLREEIKKNVFDIKSILR

EEVLSIKYL

DCVVKETLRMHPPASFISRKNKTETKLGDYDLPAGTFLRISINNVHMNESVLSWIPYLFKPER

AGAATTTAGATGTTCAAAACAAACTAAGAGAAGAAATAAAGAAAAATGTCTTTGATATAAAAAGTATT TTACGG

GAAGAAGTTTTAAGCATCAAGTACTT

GATTGTGTAGTTAAAGAGACATT

ACGCATGCATCCACCTGCGTCATTTATATCTCGAAAAAACAAAACTGAAACAAAGTTGGG

TGATTATGATCTACCTGCTGGCACGTTTTTAAGAATTTCAATTAACAACGTACATATGAA

TGAGTCTGTTTTATCCTGGATCCCTTATTTATTTAAGCCCGAACGAA

>BP514308 N-term 25% to 46a [gene 9]

1096761991009 1096082187152 1096123591182 1095899045709 1097383004013

MYSIYIAIIIVPLVFFVAVFFKRFYHQFRLLPSPKESLITCHYSYFDVHDHVNTLLNFGKEFKDYGLYTINTLI (1)

ATGTATTCGATATACATAGCGATTATAATAGTTC

CTTTAGTTTTTTTCGTTGCTGTTTTTTTTAAACGTTTTTATCATCAATTTCGTTTGTTGC

CATCACCCAAAGAGAGTTTAATTACATGTCACTATAGTTATTTTGATGTTCATGACCATG

TTAACACTCTGTTAAACTTTGGTAAAGAGTTTAAAGATTACGGATTATATACAATTAATA

CTTTAATTGGT

1096124094276 1095964247544 1095901005745 1095899045709

GPRQVHLLLPHFIKTVIADGKFFQRSPVFKAVFPLVGNSMIVSNYEDHHWQRKLF

NQAFTSQQLKRYFLAFTLHTDLLMK (0)

AGGACCCAGACAAGTTCATCTTTTATTGC

CACATTTCATTAAAACAGTAATTGCAGATGGAAAGTTTTTTCAAAGATCACCAGTTTTTA

AAGCCGTATTTCCTCTTGTTGGAAACAGTATGATCGTTTCTAATTATGAAGATCATCATT

GGCAAAGAAAATTATTTAATCAAGCCTTTACTTCGCAACAATTAAAAAGATATTTTTTAG

CTTTTACTCTGCATACTGATTTGCTAATGAAGGT

LWSCTCDKENGTNLNVWSDLSNLSFDIIGDVGFGYQFNTITSHSGNAFTKALRSY

INLRFNSSVVHNVLIAYFPFLMRFLSKFGNLNKAEQ (0)

1095958061820 82% to 1095901729505

1095964290917 1096124019775 1096528662475 1096159548758 1097622041589, 1097672472615

1096064134288 mate pair = 1096041094868 exon 3,

(0) LIDKRKKEIENGLVKEEKDFLSIVLKDQQQEKSKLTNDLIRDNLMTLLIAGHETTSTAMLWCLYTLGT (0)

AGCTTATCGATAAGCGTAAAAAAGAAATAGAAAATGGATTAGTAAAAGAAGA

GAAAGATTTTTTATCAATTGTTTTAAAAGATCAACAACAAGAAAAGAGCAAACTGACAAA

TGATTTGATTAGAGATAATTTAATGACGCTTTTAATTGCTGGTCATGAAACTACTTCTAC

TGCAATGCTGTGGTGTTTATACACATTAGGAACAGT

run into seq gap downstream

>gnl|ti|655009968 1095963046224 KYG region 46% to CYP20 35% to 27B1

(2) LGNLGSLTFDGGIHKFLVENHKRLGPMFSFYWGKELAVSLACPILFKEVATLFNRP (1)

AGATTGGGAAATCTTGGCTCTCTAACATTTGA

TGGTGGAATTCACAAGTTTCTTGTTGAAAACCATAAAAGGCTTGGTCCAATGTTCAGCTT

TTATTGGGGCAAAGAACTGGCTGTTAGTCTAGCTTGTCCAATTCTTTTTAAGGAGGTT

GCCACTCTATTTAATCGACCAGGT

>1097263613070 mate pair to 1097206643989 I-helix/J helix boundary?

1097329235455 1097672289528 1096705876537 1096110072452

LTWLVYFLCKHPEVESKVYNEIKEFTEKDLDMELLTKFS (2)

AGTATTGACATGGCTTGTTTATTTCTTATGTAAACATCCAGAAGTGGAATCTAAGGTATACA

ATGAGATAAAAGAATTTACAGAAAAAGATCTAGATATGGAATTACTTACAAAATTTAGGT

>BP508840 BP508840 Best match in Fugu, human and Ciona is CYP20

1096602177777 1097264059772 1096123966865 1097672368420

1097206643989 1096110028119

(2) YTKQVIDEVMRIAVLAPYAARYSDYDIIVDGHLIPKK (0)

(0) TPIILALGTVFQDETIFPEPDR (2)

(2) FDPDRFSDKQIEERSALAFQPFGFAGKRKCPGYRLAYAETLTYTFYIIKNFHISL

FDKQSVKMHYGFVTKPSEEIWIKVLRRKNI*

1096111030955

AGTTACACAAAGCAAGTTATTGATGAAGTTATGAGAATTGCTGTACTTGCTCCATATG

CAGCTAGATATTCGGATTATGATATAATCGTTGATGGACATCTAATACCAAAAAAGGT

AGATTTGACCCTGACCGTTTTAGTGATAAACAAATTGAAGAACG

TTCAGCGTTAGCTTTTCAGCCGTTTGGATTTGCGGGAAAAAGAAAGTGTCCTGGATATAG

ATTAGCATATGCTGAAACATTGACGTACACATTTTATATCATCAAGAATTTTCATATTTC

GCTATTCGATAAGCAATCTGTGAAAATGCATTATGGTTTTGTCACAAAACCGTCTGAAGA

AATTTGGATTAAAGTGTTACGACGTAAAAATATCTAG

>CYP20 amphioxus 39% to CYP20 Danio

MLDYAIFAITFVVFLIATVLYLYP (0)

(0) GANKITTIPGLEPSDPK (2)

(2) DGNLGDVGRAGSLHEFLLKLHTEYGDIASFWWGQQLVVSLGAPELWKQH ERIFDRP (1)

(1) ALLFKGFEPLIGAKSIQYANSVDGRTRRKLYDPSYGHNAMKHYYSIFQE (0)

(0) LGQEMAKKWESMKGDQHIPLHAHIIALAMKAITRSSFGDAFKDEKECVQFGRNYDI (0)

(0) CWNDMEERIKGSHPTEGSPREKKFKE (1)

(1) ALGKLHATIARVAKYRRENPSPPQEQLFIDVLIEGNLPEEQ (0)

(0) VLCDAMTFTVGGIHTSGN (1)

(1) LLTWALYYIATHEEVEEKLHQELSDVLGKKGEVTPDNISQLV (2)

(2) YLRQVLDESLRCAVIAPWGARYMDLDAEVGGHIVPAK (0)

(0) QTPVIHAFGVVLQDERIWPEPNK (2)

FDPDRFDAENSKGRHKLAFQPFGFAGGRKCP (1)

(1) GYRFTYTWTSVFLSILCRQFKLHLVDGQVVKPCHGLVTRPVDEIWITVTKRD*

1096111030955

ATCTCCATCAGTTAATTGCATAGATGCGAGAATGCATGTTAAGTGCATGTAACTCTGAATATTAGCGACAAAGTTTAGTATCACTATCTATGATAGTATTTTTAGTATTTATATGATTCATATTTTTCAGCATAACTCTAATAATAAATATTAATCTAAATTTTAATGCTTTTTTTTTTAAATGATTGAATATTTTTAAAACATGTATTAAATAGTTTATTAACTAAATTTTTAAAGTATTTTTAAGTACTAATAAATTTAAAAAATAAAAAAAGATGTTTATGTTTCAAAGTTTATATTTCAGTTACACAAAGCAAGTTATTGATGAAGTTATGAGAATTGCTGTACTTGCTCCATATGCAGCTAGATATTCGGATTATGATATAATCGTTGATGGACATCTAATACCAAAAAAGGTTTATACTAATATTA

>gnl|ti|646862798 1095898098005 41 0.018 35% to 17A1 34% to 2P4

gnl|ti|647168675 1095899196297

1097622027233 1096703988838 1097329365089 1097329154279

42% to 1095898227332

MLVFQQLIFAVLVPAFLYFVFSYLQHLWICSKYPKGLFPLPLLGNIH

QLGKNSSQTFSSLTKIYGDIFSVSIGTQRLVILNSMESIHEALLTKGSTFGGRPTEF

TSNVFTKGYKNLSHTDYGPNLKALRKVIHLSVQKYAGGLTRQEQMITFERDELCKKLFN

TEKEIALRCEI (1)

ATGCTTGTTTTTCAACAATTAATATTCGCCG

TACTTGTTCCGGCTTTTTTATATTTTGTTTTTTCTTATTTGCAACATTTATGGATTTGTA

GTAAGTACCCAAAGGGTCTGTTTCCATTACCGTTGTTAGGAAACATTCATCAATTAGGTA

AAAACTCTTCTCAAACATTTTCATCTTTAACAAAAATTTATGGAGATATATTTAGTGTGA

GTATTGGTACCCAGCGACTCGTTATACTCAATAGTATGGAAAGCATACATGAAGCTTTGTTAACCAAAGGTTCAACTT

TTGGTGGTAGACCAACTGAgGTTACGTCAAATGTTTTTACAAAAGGATATAAAAACTTATC

GCACACTGATTATGGACCGAATTTAAAAGCGTTGCGAAAAGTTATTCATCTTTCCGTTCA

AAAATATGCTGGCGGACTAACGAGACAAGAACAGATGATAACTTTTGAAAGAGACGAACT

TTGTAAAAAACTTTTTAATACTGAAAAGGAAATAGCTTTACGTTGTGAAATTGGT

(1) DFCTVNVMSGYLFNERFLNQNSEFKDVVKSIQLLLDNSGITDKTTFIHWLRYLPLREWN

793 EIKQARLVLNPWVEKKVEDHWRKYNENEIINVTDSMIQHFLTKYDGLDTDFAKKYITLL 617

616 LIELLVAGTETTAITICWMVLYLIHNPEYQEEIYKEITLNIGCRYPTLAEKNLFPLL 446

445 QAFIQETLRITSVVPLNLAHKALKDTSICGKIIPKDAIVITNLWNLHHDNRYFKNPNEFD 266

265 PKRWINENGLFDSISQKYFKPFSAGARVCLGETLAKNQLFLIISGLIMNFIFTSAPGKD 89

88 LPSLEGQFGITFRPNSFKVL* 29

AGATTTTTGCACTGTAAATGTAATGTCGGGGTATTTATTCAATGAACGCTTTCTGAACCAAAATTCCG

AGTTTAAAGATGTCGTAAAAAGTATTCAACTTTTGCTAGATAACTCTGGAATTACAGATA

AAACCACGTTCATACATTGGCTTCGTTACTTGCCATTGCGGGAATGGAATGAAATAAAAC

AAGCGAGACTTGTCTTAAACCCGTGGGTCGAAAAAAAGGTTGAAGATCATTGGAGAAAGT

ATAATGAAAATGAAATCATTAATGTAACTGATAGCATGATTCAACATTTTTTAACAAAGT

ACGATGGTTTAGACACTGATTTTGCAAAGAAATACATTACCTTATTATTGATCGAATTAC

TTGTTGCCGGTACCGAAACGACAGCTATTACTATTTGCTGGATGGTTTTATATCTAATAC

ATAACCCTGAGTATCAAGAAGAAATTTATAAAGAAATTACATTAAATATTGGTTGTAGAT

TGCGAATAACATCTGTTGTGCCACTAAACTTGGCTCACAAAGCATTAAAAGATACCAGCA

TTTGTGGAAAAATTATTCCTAAAGACGCTATAGTAATTACAAATTTATGGAATCTTCATC

ACGACAACAGATACTTTAAAAATCCTAATGAATTTGATCCTAAACGCTGGATAAACGAAA

ATGGTCTATTTGACTCAATTTCTCAAAAATATTTTAAACCTTTTTCGGCTGGAGCGAGAG

TATGTCTTGGCGAGACATTAGCCAAAAATCAACTTTTTTTAATCATCTCCGGTCTAATTA

TGAATTTTATTTTCACATCTGCACCAGGAAAAGACTTACCTAGTCTTGAAGGACAATTTG

GAATCACATTCCGTCCCAATAGTTTTAAGGTTTTATAA

>gnl|ti|651477674 1095901303788 39 0.11 39% to CYP21 39% to 2R1 40% to 2P4

49% to 1095898227332

1096703646566 mate pair = 1096703498438 = N-terminal exon

1097675091467

MFLFVVFEVVFGLIIPVLLYVI

VVYIYHIWECQRYPPGPFPLPVIGNYNLLANDPVKALCDLEIIYGDVFSLSLGTVR

VVVVSSHESIYDVLVGDGSNFSGRPREYSSLLFTGGFENLSHMDNNPLTKKIRKVFYSKL

KTNGSILAHNENIVKHESELLHQRLLQNEGSVTNLRYEI (1)

(1) DLCIVNSICSIIFGNRLSDTCEVHEILKATRLLLKNLSNIEIMHYLPWM

RFFLLKKQNEISESRNICKFWIQTQLHKRKKSLKNENISDILLNLWDQQKQENP

NEEQYRMILVELVMAGSETTAATITWLIFYLLHWPHYQSILYKEIKNVCGDQYPTFNDIKSMPIMQATILE

TLRLSSVVPLSLSHKAVNNAKINKFTIPKDTIIITNLWGVHHNEKYWEKPFEFNPMRWLD

KNGELSTAKRLGYFPFSAGPRGCIGESFARMQMFIICSRLIKDFSFELPQSGETPKLDGD

IGITLTPLPYNAVAKQRT*

ATGTTTCTTTTTGTAGTTTTTGAAGTTGTATTTGGGCTGATAATTCCCGTTTTACTTTACGTAATAGTT

GTTTATATTTATCATATTTGGGAATGTCAAAGATACCCACCAGGT

CCATTTCCTCTTCCGGTAATTGGAAACTACAACTTGTTAGCAAATGATCCTGTGAAGGCA

TTGTGCGATCTAGAAATTATTTACGGAGATGTTTTCAGTTTAAGTTTAGGAACCGTTCGG

GTGGTTGTTGTAAGCAGCCACGAGAGTATTTACGATGTTTTAGTTGGAGATGGATCAAAT

TTTTCCGGAAGACCCAGGGAGTATTCATCTTTACTTTTTACTGGAGGTTTTGAAAACCTT

TCCCATATGGATAACAACCCGTTGACTAAAAAAATCAGAAAAGTTTTTTATTCAAAACTT

AAAACAAACGGAAGTATTTTAGCACACAATGAAAATATTGTCAAACATGAAAGTGAACTT

TTACATCAAAGACTACTGCAAAACGAAGGAAGCGTCACCAATCTTCGTTATGAAATCGGT

AGATCTTTGTATTGTTAACAGCA

TATGCAGTATTATTTTTGGTAACCGGCTTAGTGATACTTGTGAAGTTCATGAAATTTTAA

AAGCGACCAGGTTACTTCTAAAAAACTTGTCAAACATTGAAATTATGCATTATTTACCAT

GGATGAGATTTTTTTTATTAAAAAAGCAAAACGAAATCAGCGAATCTAGAAACATTTGCA

AATTTTGGATTCAAACCCAGTTGCATAAACGAAAAAAAAGTTTAAAAAACGAAAATATCT

CAGATATTCTTTTGAACCTTTGGGACCAACAAAAACAAGAAAACCCTAATGAGGAACAAT

ACAGAATGATTTTAGTTGAGTTAGTTATGGCTGGTTCCGAAACAACAGCCGCAACGATAAC

TTGGCTAATCTTTTATCTTTTGCATTGGCCTCACTATCAAAGCATTCTTTACAAAGAAAT

CAAAAATGTTTGTGGTGATCAGTACCCTACGTTTAATGATATTAAATCAATGCCTATAAT

GCAAGCAACTATACTTGAAACTTTAAGGTTGTCTTCTGTCGTTCCTTTAAGCTTATCTCA

CAAAGCCGTAAATAACGCGAAAATTAATAAATTCACAATCCCTAAAGATACAATAATAAT

AACAAATTTATGGGGCGTACATCATAATGAAAAATACTGGGAAAAACCGTTTGAATTCAA

TCCTATGCGTTGGCTTGATAAAAATGGCGAACTTTCAACAGCAAAGCGTTTAGGATATTT

CCCTTTTTCAGCCGGCCCAAGAGGTTGCATTGGTGAGTCATTTGCAAGAATGCAAATGTT

TATTATATGCTCTCGACTGATAAAAGATTTCTCCTTTGAGTTGCCTCAAAGCGGAGAAAC

CCCAAAACTAGATGGTGATATTGGAATTACACTAACGCCCCTTCCTTATAATGCAGTAGC

TAAACAGCGAACCTAA

>gnl|ti|654998190 1095901734433 33% to CYP21 33% to CYP17 33% to 2U1

gnl|ti|651148169 1095901003210

gnl|ti|651162328 1095901096755

74% to 1095898227332

possible pseudogene

870 FQDIIKTHNET

837 SYISSIPWLRYFPTATSRNMIILNKNKYNIFEIIRLRDPILIRKLQEHKRTYD*SNLGDV 658

657 TDALIKISLESVTETDSHEKITDDNTEFLLNDFMIAGSETSSNMILWFIVYILHRPEYQDKLYDEIFKVFSG

IGYPSLNDRPRFHLIQAIIHETLRLLSVAPLGLCHKALENGSICGKFVPKG (frameshift)

LLILTNLWSIHHDERYWKNAINFFPERWLDNSGNFNYNLGYAYLPFS 147

146 GGPRSCLGETLAKTELFVFISRLVKDYRFEKPNGKDSLDGRSGVTCLPYEFEIVMIPRS*

>gnl|ti|655009845 1095963045220 near C-helix region poor match

gnl|ti|648592188 1095595897239

NOT A P450, MATCHED FISH AND DROSOPHILA SEQUENCES

637 STSNCVTMNQSDYLQNVLQKFGFDNCKQRSSPCKQVPYSYHNQESIKSNGSMIKL 473

472 YRQMVGSLLYAMTCTRPDLSYVITKLSQHLSKPNSGDWIMIKHVFRYIKHTLN 314

313 YCLTFRK 293

>gnl|ti|648047811 1095899057643 I-helix 4 aa diffs to 1095898198167

(0) LIEKRKKEIDDGISTKEKDIITIVLKDQQQESSKLTNDLIRDNLLLFLIAGHKTTSTTMTWCLYILGT (0)

AGCTTATTGAAAAACGTAAAAAAGAAATCGACGATGGAATATCAACAAAAGAGA

AGGATATTATCACAATTGTCTTAAAAGATCAACAGCAAGAAAGCAGCAAACTAACAAATG

ATTTGATTAGAGATAATTTATTATTATTTCTCATAGCTGGTCATAAAACAACTTCTACTA

CTATGACTTGGTGTTTATATATACTAGGCACTGT

CYP1 like (only one seq)

1095899272864 5 aa diffs in N-term overlap region

MWYEIICGLIISILLYIIGSYLMHLLECRKYPLGPFPIPIFGNLHLLGTEPHKILAAYS

KKYGAVFSISLGLQRIVIISDITTTREALVQKASIFAGRPKSYLIQLISSGYKGIAFMDY

GSFWKVLRKVSHSSLKIYGEGHERFEKILTKESEELHKRLLKKSNNSVELKSEF (1)

>gnl|ti|648485307 1095899272864 57% to 1095897342515

ATGTGGTATGAAAT

TATCTGCGGACTGATCATTTCGATTTTGCTATATATTATTGGTTCTTACTTGATGCACTT

GCTGGAATGCAGGAAGTATCCTCTTGGACCTTTTCCAATACCAATCTTTGGTAACTTGCA

TTTATTAGGAACAGAGCCACATAAAATACTTGCTGCATACTCAAAAAAGTATGGAGCAGT

CTTTAGCATAAGTTTAGGATTGCAAAGAATTGTTATAATTTCTGACATTACTACAACTAG

AGAAGCACTAGTTCAAAAAGCATCCATATTTGCAGGTAGACCAAAATCTTATTTAATTCA

ATTAATTTCAAGTGGGTACAAAGGCATTGCATTTATGGACTATGGTTCCTTCTGGAAAGT

TTTGCGTAAAGTTAGTCATTCTTCATTAAAAATATATGGAGAAGGACATGAACGTTTTGA

AAAGATACTTACAAAAGAAAGTGAAGAGCTACATAAAAGACTTTTAAAGAAATCAAATAA

TTCCGTAGAGCTGAAATCTGAATTTGGT

>CN775634

tae83e09.y1 Hydra EST Darmstadt I Hydra magnipapillata cDNA 5' similar to

SW:CP11_OPSTA Q92095 CYTOCHROME P450 1A1

AGAGAGTGAAGAGCTACATAAAAGACTTTTAATGAAATCAAAAACTTCCGTAGATCTGAAAACTGAATTT

GGTGCTGCAATTATAAACGTGATTTGTTTCATTGTTTTTGGGGAAAGATACCAGTATTCAAATTCAGAAT

TTAAAGAAGTTCTTACAACAATAAACAATATAGTCGATGGGTTGTCAAATACAACTGCTGTCGGTTTTTT

GCCGTGGTTGAGATTTTTACCGTTTTCTCCAATAAAAAAACTGAGTATTTCACTTTCAAAATATATTCGT

TTTTTAAACGATAAGTTGACCAAACATAAGGAAACATTTAATGAAAACAAAATTCGAGATTCTACTGATT

CTATTATAAAC

32% to CYP1C1 aa 173-297

2 ESEELHKRLLMKSKTSVDLKTEF (1)

GAAIINVICFIVFGERYQYSNSEFKEVLTTINNIV 175

176 DGLSNTTAVGFLPWLRFLPFSPIKKLSISLSKYIRFLNDKLTKHKETFNENKIRDS 343

344 TDSIIN 361

opposite end of clone =

>CN774619

tae83e09.x1 Hydra EST Darmstadt I Hydra magnipapillata cDNA 3' similar to

SW:CPT7_CHICK P12394 CYTOCHROME P450 17

TTTAAACTACATAAGATTGTTTACTCTTGGAATCAAGTATGCGCAAAATTGTTTTGGAGCATGAGTAATA

TTGCATTTTCCAATTAAACTTGGAGGTGGACAACCAGGTGTGCACTCAAACTTAAAATCTCGAATTAATC

TAGAAAAGAAGAAAAATAGTTCATTTTCAGCAACTGTTTTCCCTAAACAAACTCTTGTACCGGCTGAAAA

AGGTAAGAAACTTGTTGCTTTACTTGGATCAAATTTCTTATCTTTGCCAATCCGCCGATAAGGAATTAAA

TTCATTAGGATTTTCCCAGGAGTTAGTAACGTTATGAATCTGCCAATGATTAATCATGACAGTAGCAATT

TTCAGGAATGCTATGTGACATGAGAACTCCCCCCCAGAGGT

38% to CYP17A2 Fugu aa 383-485

391 TSGGEFSCHIAFLKIATVMINHWQIHNVTNSWENPNEFN 275

273 PYRRIGKDKKFDPSKATSFLPFSAGTRVCL

(1) GKTVAENELFFFFSRLIRDFKFECTPGCPP 94

93 PSLIGKCNITHAPKQFCAYLIPRVNNLM* 7

1097672474909

AGGGAAA GTTGCTGAAAATGAACTATTTTTC TTCTTTTCTAGATTAATTCGAGATTTTAAGTTTG

AGTGCACACCT GGTTGTCCACCTCCAAGTTTAGTTGGAAAATGCAATATTACTCATGCT

CCAAAACAATTTTGCGCATACTTGATTCCAAGAATAAACAATCTTATGTAG