LISA K. JENNINGS
ASSOCIATE PROFESSOR

EDUCATION:
B.A. 1976, University of Tennessee, Knoxville, Tennessee
M.S. 1978, Memphis State University, Memphis, Tennessee
Ph.D. 1983, University of Tennessee, Memphis, Tennessee
Postdoctoral 1983-1985, St. Jude Children's Research Hospital, Memphis, Tennessee

RESEARCH INTERESTS: The biochemistry of human platelet function is the major area of focus of our laboratory research. I am interested in the role of receptors in initiating platelet response and how these receptor proteins participate in platelet activation mechanisms, platelet aggregation and post receptor occupancy events. Our studies are designed to define events important in hemostasis and thrombosis and also to understand the pathophysiology of acquired or inherited platelet function defects.

CURRENT RESEARCH SUPPORT:
American Heart Association Established Investigatorship Award, July 1, 1991 - June 30, 1996
COR Therapeutics, Sponsored Research Contract, September 1, 1993 - August 31, 1996
IMMUNO-AG, Sponsored Research Contract, September 1, 1995 - August 31, 1996

GRADUATE STUDENTS: Bill Kouns, Ph.D., 1991; Denise Wall, Ph.D., 1993; Traci Heath, Ph.D., 1994; Joseph Crossno (current student)

PUBLICATIONS: (selected)
1. Phillips, D.R., Jennings, L.K., and Edwards, H.H. (1980) Identification of membrane proteins mediating the interaction of human platelets. J. Cell Biol., 86, 77.
2. Phillips, D.R., Jennings, L.K., and Prasanna, H.R. (1980) Ca2+-mediated association of glycoprotein G (thrombin-sensitive protein, thrombospondin) with human platelets.
J. Biol. Chem., 225, 1629.
3. Jennings, L.K., Fox, J.E.B., Edwards, H.H., and Phillips, D.R. (1981) Changes in the cytoskeletal structure of human platelets following thrombin activation. J. Biol. Chem., 256, 6927.
4. Jennings, L.K. and Phillips, D.R. (1982) Purification of glycoproteins IIb and III from human platelet plasma membranes and characterization of a calcium-dependent glycoprotein IIb-III complex. J. Biol. Chem., 257, 10458.
5. Phillips, D.R., Fujimura, K., Jennings, L.K., Parise, L., Fitzgerald, L., and Fox, J.E.B. (1983) Calcium regulation of glycoproteins IIb and III in human platelet membranes.
Ann. N.Y. Acad. Sci., 416, 166.
6. Jennings, L.K., Brown, L.K., and Dockter, M.E. (1985) Quantitation of protein 3 content in circulating erythrocytes at the single cell level. Blood, 65, 1256.
7. Jennings, L.K., Ashumn, R.A., Wang, W., and Dockter, M.E. (1986) Analysis of human platelet glycoproteins IIb and IIIa and glanzmann's thrombasthenia in whole blood by flow cytometry. Blood, 68, 173-179.
8. Singer, J., Jennings, L.K., Jackson, C., Dockter, M.E., Morrison, M., and Walker, W.S. (1986) Red blood cell homeostasis: Antibody mediated recognition of the senescent state by macrophages. PNAS, 83, 5498-5501.
9. Loftus, J.C., Plow, E.F., Jennings, L.K., and Ginsberg, M.H. (1988) Alternative proteolytic processing of platelet GP IIb. J. Biol. Chem., 263, 11025.
10. Jackson, C.W. and Jennings, L.K. (1989) Heterogeneity of fibrinogen receptor expression on platelets activated in normal plasma with ADP. Analysis by flow cytometry. Br. J. Hemat., 72, 407.
11. Jennings, L.K., Dockter, M.E., Wall, C.D., and Fox, C.F. (1989) Calcium mobilization in human platelets using do-1 and flow cytometry. Blood, 74, 2674.
12. Jennings, L.K., Fox, C.F., Kouns, W.C., McKay, C.P., Ballou, L.R., and Schultz, H.E. (1990) The activation of human platelets mediated by anti-human platelet p24/CD9 monoclonal antibodies. J. Biol. Chem., 265, 3815.
13. Kouns, W.C., Wall, C.D., White, M.M., Fox, C.F., and Jennings, L.K. (1990) A conformation dependent epitode of human platelet glycoprotein IIIa. J. Biol. Chem., 265, 20594.
14. Jennings, L.K., Wang, W.C., Jackson, C.W., Fox, C.F., and Bell, A. (1991) Hemostasis in glanzmann's thrombasthenia (GT): GT platelets interfere with the aggregation of normal platelets. Am. J. Ped. Hem. Onc., 13, 84.
15. Rubenstein, E., Kouns, W.C., Jennings, L.K., Boucheix, C., and Carroll, R.C. (1991) Interaction of two GPIIb/IIIa monoclonal antibodies with platelet Fc receptor (FCgRII).
Br. J. Haematol., 78, 80.
16. Lanza, F., Wolf, D., Fox, C.F., Kieffer, N., Seyer, J.M., Fried, V.A., Coughlin, S.R., Phillips, D.R., and Jennings, L.K. (1991) cDNA cloning and expression of platelet p24/CD9: Evidence for a new family of multiple membrane spanning proteins. J. Biol. Chem., 266, 10638.
17. Kouns, W.C. and Jennings, L.K. (1991) Activation-independent exposure of the GPIIb-IIIa fibrinogen receptor. Thromb. Res., 63, 343.
18. Kouns, W.C., Fox, C.F., Lamoueaux, W.J., Coons, L.B., and Jennings, L.K. (1991) Aggregation dependent alterations of triton X-100 insoluble residues isolated from ADP-stimulated platelets in plasma. J. Biol. Chem., 266, 13891.
19. Kouns, W.C., Newman, P.J., Puckett, K.J., Miller, A.A., Wall, C.D., Fox, C.F., Seyer, J.M., and Jennings, L.K. (1991) Further characterization of the loop structure of platelet glycoprotein IIIa: Partial mapping of functionally significant epitopes. Blood, 78, 3215.
20. White, M.M., Foust, J.T, Mauer, A.M., Robertson, J.T., and Jennings, L.K. (1992) Assessment of luminaggregometry for research and clinical laboratories.
Thrombosis and Haemostasis, 67, 572.
21. Kouns, W.C., Kirchhofer, D., Hadvary, P., Edenhofer, A., Weller, T., Pfenninger, G., Baumgartner, H.R., Jennings, L.K., and Steiner, B. (1992) Reversible conformational changes induced in GPIIb-IIIa by a potent and selective peptidomimetic inhibitor. Blood, 80(10), 2539.
22. Jennings, L.K., White, M.M., Sauer, C.M., Mauer, A.M. and Robertson, J.T. (1993) Cocaine-induced platelet defects. Stroke, 24, 1352.
23.Jennings, L.K., Crossno, Jr., J.T., Fox, C.F., White, M.M., and Green, C.A. (1994) Platelet p24/CD9, a member of the tetraspanin family of proteins. Ann. N.Y. Acad. Sci., 714, 175-184.
24.Slack, S.M., Jennings, L.K., and Turitto, V.T. (1994) Platelet size distribution measurements as indicators of shear stress induced platelet aggregation. Annals. Biomed. Eng., 22(6), 653.
25.Wall, J.E., Buijs-Wilts, M., Arnold, J.T., Wang, W., White, M.M., Jennings, L.K., and Jackson, C.W. (1995) A flow cytometric assay using mepacrine for study of uptake and release of platelet dense granules. Brit. J. Haematol., 89, 380.
26.Jennings, L.K., White, M.M., and Mandrell, T.D. (1995) Interspecies comparison of platelet aggregation, LIBS expression and clot retraction: Observed differences in GPIIb-IIIa functional activity. Thromb. Haemos., in press.