JOHN N. FAIN
VAN VLEET PROFESSOR AND CHAIRMAN

EDUCATION:
B.S. 1956, Carson-Newman College, Jefferson City, Tennessee
Ph.D. 1960, Emory University, Atlanta, Georgia
Postdoctoral 1961-65, National Institutes of Health, Bethesda, Maryland

RESEARCH INTERESTS: The general field of signal transduction is the focus of work in my laboratory, and I am currently examining the regulation of metabolism in adipocytes. Obesity is an increasing health problem in this country and excess calories are stored in adipocytes. The mobilization of lipid stored in adipocytes is known as lipolysis, and I am examining the regu-lation of lipolysis by catecholamines, which are thought to be the major short-term physiological regulators of lipolysis. Recently renewed interest in adipocyte metabolism has arisen because of the finding that obese-hyperglycemic mice do not make a protein in their adipocytes known as leptin, which appears to regulate food intake. Current emphasis in my laboratory is on the regulation of b1 catecholamine receptor function by phosphorylation, the effects of thyroid hormones on the b1 and b3 adrenergic receptors, and leptin mRNA regulation in rat adipocytes.

GRADUATE STUDENTS:
Brown University: Jay Moskowitz, Ph.D., 1969; Nora Reed Hackett, Ph.D., 1969; Richard Saperstein, Ph.D., 1970; Susan Collins Loken, Ph.D., 1971; Judith Wolder Rosenthal, Ph.D., 1971; Michael P. Czech, Ph.D., 1972; Yvonne Clement-Cormier, Ph.D., 1974; Margaret E.M. Tolbert, Ph.D., 1974; Richard H. Pointer, Ph.D., 1975; Morris J. Birnbaum, Ph.D., 1976; Sue-Hwa Lin, Ph.D., 1983; Paul Randazzo, Ph.D., 1986
University of Tennessee, Memphis: Horng-Mo Lee, Ph.D., 1991.

PUBLICATIONS: Selected publications appearing since 1985 are listed.
168. Litosch, I., Wallis, C., and Fain, J.N. (1985) 5-Hydroxytryptamine stimulates inositol phosphate production in cell-free system from blowfly glands: Evidence for a role of GTP in coupling receptor activation to phosphoinositide breakdown. J. Biol. Chem., 260, 5464-5471.
179. Mills, L., Garcia-Sainz, J.A., and Fain, J.N. (1986) Pertussis toxin effects on adenylate cyclase activity, cyclic AMP accumulation and lipolysis in adipocytes from hypothyroid, euthyroid and hyperthyroid rats. Biochim. Biophys. Acta, 876, 619-630.
193. Pittner, R.A., and Fain, J.N. (1989) Vasopressin transiently stimulates phospholipase C activity in cultured rat hepatocytes. Biochim. Biophys. Acta, 1010, 227-232.
194. Lee, H.-H. and Fain, J.N. (1989) Regulation of oxytocin-induced phosphoinositide breakdown in adipocytes by adenosine, isoproterenol and insulin. Biochim. Biophys. Acta, 1013, 73-79.
195. Claro, E., Wallace, M.A., Lee, H-M., and Fain, J.N. (1989) Carbachol in the presence of GTPgS stimulates the breakdown of exogenous phosphatidylinositol 4,5-bisphosphate, phosphatidylinositol 4-phosphate and phosphatidylinositol by rat brain membranes. J. Biol. Chem., 264, 18288-18295.
196. Etindi, R. and Fain, J.N. (1989) Insulin does not activate a phosphoinositide-specific phospholipase C in adipocytes. Mol. and Cellular Endocrinology, 67, 149-153.
197. Pittner, R.A. and Fain, J.N. (1990) Vasopressin and norepinephrine stimulation of inositol phosphate accumulation in rat hepatocytes are modified differently by protein kinase C and protein kinase A. Biochim. Biophys. Acta, 1043, 211-217.
198. Pittner, R.A. and Fain, J.N. (1990) Effects of insulin on inositol phosphate production in cultured rat hepatocytes. Biochim. Biophys. Acta, 1043, 218-224.
199. Claro, E., Wallace, M.A. and Fain, J.N. (1990) Dual effect of fluoride on phosphoinositide metabolism in rat brain cortex: Stimulation of phospholipase C and inhibition of polyphosphoinositide synthesis. Biochem. J., 268, 733-737.
205. Lee, H-M. and Fain, J.N. (1991) Phorbol myristate acetate inhibition of phospholipase C activation by carbachol in slices of rat brain cortex is a delayed and indirect effect. J. Neurochem., 56, 1471-1480.
210. Pittner, R.A. and Fain, J.N. (1991) Activation of membrane protein kinase C by glucagon and Ca++ mobilizing hormones in cultured rat hepatocytes. Role of phosphatidylinositol and phosphatidylcholine hydrolysis. Biochemical J., 277, 371-378.
212. Claro, E., Wallace, M.A., Fain, J.N., Nair, B.G., Patel, T.B., Shanker, G., and Baker, H.J. (1991) Altered phosphoinositide-specific phospholipase C and adenylyl cyclase in brain cortical membranes of cats with GM1 and GM2 gangliosidosis. Molecular Brain Research, 11, 265-271.
215. Llahi, S., Claro, E., and Fain, J.N. (1992) Quisqualate-stimulated phosphatidylinositol breakdown in cerebellar membranes. J. Neurochemistry , 58, 714-721.
216. Claro, E., Wallace, M.A., and Fain, J.N. (1992) Concerted CMP-dependent [3H]inositol labelling of phosphoinositides and agonist activation of phospholipase C in rat brain cortical membranes. J. Neurochemistry, 58, 2155-2161.
217. Llahi, S., Claro, E. and Fain, J.N. (1992) a-1 adrenergic receptor-mediated activation of phospholipase D in rat cerebral cortex. J. Biol. Chem., 267, 3679-3685.
218. Lee, H.-M. and Fain, J.N. (1992) Magnesium dependent inhibition of agonist-stimulated phosphoinositide breakdown in rat cortical slices by excitatory amino acids. J. Neurochemistry, 59, 953-962.
219. Salles, J., Wallace, M.A., and Fain, J.N. (1993) Differential effects of alkylating agents on multiple muscarinic receptor subtypes linked to activation of phospholipase C in rat brain cortical membranes. J. Pharmacol. Exp. Therap., 264, 521-529.
220. Claro, E., Fain, J.N. and Picatoste, F. (1993) Noradrenaline stimulation unbalances the phosphoinositide cycle in rat cerebral cortical slices. J. Neurochemistry, 60, 2078-2086.
222. Kang, E. S., Betts, D., Fain, J.N., Bahouth, S.W., Myers, L.K. (1993) Chronic exposure of rat fat cells to insulin enhances lipolysis and the activation of partially-purified hormone-sensitive lipase. Diabetes, 42, 1415-1424.
223. Fain, J.N. (Ed.). (1993) Lipid metabolism in signaling systems. Vol. 18 in Methods in Neurosciences. (28 vols). San Diego: Academic Press.
225. Claro, E., Picatoste, and Fain, J.N. (1993) Comparison of different procedures for demon-stration of agonist stimulation of phosphoinositide breakdown in brain membranes. In J.N. Fain (Ed.), Methods in Neurosciences, Vol. 18 (pp. 38-48). San Diego: Academic Press.
226. Myles, M.E. and Fain, J.N. (1994) Carbachol but not norepinephrine, NMDA, ionomycin, ouabain or phorbol myristate acetate increases inositol 1,3,4,5 tetrakisphosphate accumulation in rat brain cortical slices. J. Neurochemistry, 62, 2333-2339.
227. Myles, M.F., Gokmen-Polar, Y., and Fain, J.N. (1995) Inhibition by veratridine of carbachol stimulated inositol tetrakisphosphate accumulation in rat brain cortical slices. Neurochemical Research, 20, 1057-1064.
230. Gokmen-Polar, Y., Coronel, E.C., Bahouth, S.W., and Fain, J.N. Insulin sensitizes b-agonist and forskolin-stimulated lipolysis to inhibition by dideoxyadenosine. Am. J. Physiol., in press.