Last modified Mar. 24, 1999 There are at least 18 DICTYOSTELIUM DISCOIDIUM (SLIME MOLD) P450 SEQUENCES based on comparing the C-terminal sequences. SEQUENCE 1 40-43% identical to CYP73 sequences SEQUENCE 1 AND SEQUENCE TWO ARE FROM THE SAME GENE BASED ON TWO CLONES SSJ655 AND SLB521 C92049 D. disc. I-helix to end CLONE SSC117 PMVLMLTNYPEIQEKAFDEIKTVVNGRSKVNLSDRQSTPYLVAVIKETLR YKPMSPFGLPRSSSKDCMIGGHFIPKNAQILINYQALGMNEEYYENPEQF DPSRFLKVESNVAFLPFSIGIRSCVGQSFAQDELYICISNILLNFKLKSI DGKKIDETEEYGLTLKTKNRFNVTLEKRII* C89925 D. disc I-helix to end CLONE SSG630 QVQLL*NQWY*C*QIIPEIQEKAFDEIKTVVNGRSKVNLSDRQSTPYLVA VIKETLRYKPMSPFGLPRSSSKDCMIGGHFIPKNAQILINYQALGMNEEY YENPEQFDPSRFLKVESNVAFLPFSIGIRSCVGQSFAQDELYICISNILL NFKLKSIDGKKIDETEEYGLTLKTKNRFNVTLEKRII* C90052 D. disc. I-helix to end CLONE SSG143 LMLTNYPEIQEKAFDEIKTVVNGRSKVNLSDRQSTPYLVAVIKETLRYKP MSPFGLPRSSSKDCMIGGHFIPKNAQILINYQALGMNEEYYENPEQFDPS RFLKVESNVAFLPFSIGIRSCVGQSFAQDELYICISNILLNFKLKSIDGK KIDETEEYGLTLKTKNRFNVTLEKRII* C91122 D. disc. I-helix to end CLONE SSJ655 ATINDFFLAGVDTSSTALESMVLMLTNYPEIQEKAFDEIKTVVNGRSKVN LSDRQSTPYLVAVIKETLRYKPMSPFGLPRSSSKDCMIGGHFIPKNAQIL INYQALGMNEEYYENPEQFDPSRFLKVESNVAFLPFSIGIRSCVGQSFAQ DELYICISNILLNFKXKSIDGKKIDETEEYGLTLKTKNRFNVTLEKRII* C25600 D. disc. I-helix to end CLONE SSA247 KVNLSDRQSTPYLVAVIKETLRYKPMSPFGLPRSSSKDCMIGGHFIPKNA QILINYQALGMNEEYYENPEQFDPSRFLKVESNVAFLPFSIGIRSCVGQS FAQDELYICISNILLNFKLKSIDGKKIDETEEYGLTLKTKNRFNVTLEKR II* C94043 Dictyostelium discoideum slug CLONE SSG323 RQSTPYLVAVIKETLRYKPMSPFGLPRSSSKDCMIGGHFIPKNAQILINYQALGMNEEYY ENPEQFDPSRFLKVESNVAFLPFSIGIRSCVGQSFAQDELYICISNILLNFKLKSIDGKK IDETEEYGLTLKTKNRFNVTLEKRI C94448 D. disc. CLONE SSK816 VDTSSTALESMVLMLTNYPEIQEKAFDEIKTVVNGRSKVNLSDRQSTPYLVAVIKETLRY KPMSPFGLPRSSSKDCMIGGHFIPKNAQILINYQALGMNEEYYENPEQFDPSRFLKVESN VAFLPFSIGIRSCVGQSFAQDELYICISNILLNFKLKLIDGKKIDETEEYGLTLKTKNRF NVTLEKRI 575 AU034703 Dictyostelium discoideum CLONE SLE212 LATINDFFLAGVDTSSTALESMVLMLTNYPEIQEKAFDEIKTVVNGRSKVNLSDRQSTPYL VAVIKETLRYKPMSPFGLPRSSSKDCMIGGHFIPKNXXILINYQALGMNEEYYENPEQFD PSRFLKVESNVAFLPFSIGIRSCVGQSFAQDELYICISNILLNFKLKSIDGKKIDETEEY GLTLKTK AU033852 Dictyostelium discoideum CLONE SLB521 PEIQEKAFDEIKTVVNGRSKVNLSDRQSTPYLVAVIKETLRYKPMSPFGLPRSSSKDCMI GGHFIPKNAQILINYQALGMNEEYYENPEQFDPSRFLKVESNVAFLPFSIGIRSCVGQSF AQDELYICISXILLNFKLKSIDGKKIDETEEYGLTLKTKNRFNVTLEKRII AU037735 Dictyostelium discoideum CLONE SSE231 TALESMVLMLTNY PEIQEKAFDELKTVVNGRSKVNLSDRQSTPYLVAVIKETLRYKPMSPFGL PRSSSKDCMIGGHFIPKNAQILINYQALGMNEEYYENPEQFDPSRFLKVESNVAFLPFSI GIRSCVGQSFAQDELYICISNILLNFKLKSIDGKKIDETEEYGLTLKTKNRFNVTLEKRII AU034252 Dictyostelium discoideum CLONE SLC329 LRYKPMSPFGLPRSSSKDCMIGGHFIPKNAQILINYQALGMNEEYYENPEQFDPSRFLKV ESNVAFLPFSIGIRSCVGQSFAQDELYICILNILLNFKLKSIDGKKIDETEEYGLTLKTK NRFNVTLEKRII AU037979 Dictyostelium discoideum CLONE SSH119 PYLVAVIXETLRYKPMSPFGXPRSSSKDCMIGGHFIPKNAQILINYQALGMNEEYYENPE QFDPSRFLKVESNVAFXPFSIGIRSWV GQSFAQDELYICISNILLNFKLKSIDGKKIDETEEYGLTLKTKNXFNVTLEKRII SEQUENCE 2 34% identical to 73A14 35% identical to Zinnia elegans CYP73 C92378 D. disc. N-terminal to sequence 1 above CLONE SSE715 MALFEIIISLFVVYIIHNAISKYKKIHVNELCGPTPIPILGNLHQFGELPH RVLTKMTKKYGHILRVYMADMYTVVVSDPLLIREMYVD NSDIFTDRVKKPSVEHGTFYHGTVTSYGEHWKNNREIVGKAMRKTNLKHI YELLDKQVDVLIRSMKSIETSGKTFDTRYYITKFTMSAMFKFLFNHDIPE DEDINKGDTQKKKX C24684 N-terminal CLONE SSA192 MALFEIIISLFVVYIIHNAISKYKKIHVNELCGPTPIPILGNLHQFGELPH RVLTKMTKKYGHILRVYMADMYTVVVSDPLLIREMYVD NSDIFTDRVKKPSVEHGTFYHGTVTSYGEHWKNNREIVGKAMRKTNLKHI YELLDKQVDVLIX CONSENSUS SEQUENCE CYP508 OF SEQS 1 AND 2 COMPLETE SEQUENCE MALFEIIISLF VVYIIHNAISKYKKIHVNELCGPTPIPILGNLHQFGELPHRVLTKMTKKYGHILRVYMAD MYTVVVSDPLLIREMYVDNSDIFTDRVKKPSVEHGTFYHGTVTSYGEHWKNNREIVGKAM RKTNLKHIYELLDKQVDVLIRSMKSIET SGKTFDTRYYITKFTMSAMFKFLFNHDIPEDE DINKGDTQKLMGPMSEVFQNAGRGSLFDVINITQPLYLLYLEMFDQSFKDIMKYHREKYN EHLKTFDPDVERDLLDILIKEYGTDNDDKILSILATINDFFLAGVDTSSTALESMVLMLT NYPEIQEKAFDEIKTVVNGRSKVNLSDRQSTPYLVAVIKETLRYKPMSPFGLPRSSSKDC MIGGHFIPKNAQILINYQALGMNEEYYENPEQFDPSRFLKVESNVAFLPFSIGIRSCVGQ SFAQDELYICISNILLNFKLKSIDGKKIDETEEYGLTLKTKNRFNVTLEKRII* CQMKRANPSVSQSLRVRQLKKLMEEKAIEFIDSLEKISNNNEIVSF SEQUENCE 3 32% identical to many 1A seqs. especially fish 1As C84055 D. disc. I-helix to end CLONE SSC561 LLIEYGLNEITKEDTMQINQICFDIFGPAVGTVTITMNWVI*QLCNRPEL QEIAYXEXKKAVKDDEYVNLNHKQNAPYIVAFIKETMRLCSNGFGLPRTA KNDQICGDFFIPKDAIIFINYLEISQNEEIFKNAKEFNPTRYLDESLPVP NIHFGVGQRACPGRFVAIDKMFLGISNLLLKYKLKTQNGEKIDDSIQFSV SLKAKDYGIKLEKRI* 5 PRIME END OF CLONE SCC317. C84138 IS THE 3 PRIME READ NHLKSIDPSKPRDLMDDLLIEYGLNEITKEDTMQINQICFDIFGPAVGTVTITMNWVIL QLCNRPELQEIAYQEIKKAVKDDEYVNLNHKQNAPYIVAFIKETMRLCSNGFGLPRTAKN DQICGDFFIPKDAIIFINYLEISQNEEIFKNAKEFNPTRYLDESLPVPNIHFGVGQRACP GRFV CONSENSUS OF SEQ. 3 MEIINVFLFLIILFLVKDFVKKNKKIHTKS PSGPIAFPILGNVVQIRFWELFKIQEHELFGGYSKKYNGVVRAWFGERLFFFVSNYD VVKYFQKDENFHNRPSVLVPGWRYASSNGLGVMSSSDDKWKRAKSSVSQSLRVRTTK KLMEEKAIEFMIHLKKFQIIMKLGDP (15 amino acid gap or small intron) IFIFSFIQRTYSRICLFNVFKYMFIQDLSVESGMSRTIGNAVEHVFGNLS KLTAFDCFEIFSPLYDWFFTRRLKGCDIVRQIISSQN ENHLKSIDPSKPRDLMDD LLIEYGLNEITKEDTMQINQICFDIFGPAVGTVTITMNWVILQLCNRPEL QEIAYQEIKKAVKDDEYVNLNHKQNAPYIVAFIKETMRLCSNGFGLPRTA KNDQICGDFFIPKDAIIFINYLEISQNEEIFKNAKEFNPTRYLDESLPVP NIHFGVGQRACPGRFVAIDKMFLGISNLLLKYKLKTQNGEKIDDSIQFSV SLKAKDYGIKLEKRI* New From Jena >JAX4a82d03.r1 Clone JAX4a82d03, reverse read, bases 21 through 741, from 98-11-27 IFIFSFIQRTYSRICLFNVFKYMFIQDLSVESGMSRTIGNAVEHVFGNLS KLTAFDCFEIFSPLYDWFFTRRLKGCDIVRQIISSQN ENHLKSIDPSKPRDLMDDLLIEYGLNEITKEDTMQINQICFDIFGPAVGTVTITMNWVILQLCNRPELQEIAYQEIKKAVKDD EYVNLNHKQNAPYIVAFIKETMRLCSNGFGLPRTAKNDQIWGIFSFQKSYYFINYLEIRPKWKKSLKC >JC2b115g01.r1 Clone JC2b115g01, reverse read, bases 43 through 498, from 99-10-06 frame -1 *CQMKRANPSVSQSLRVRQLKKLMEEKAIEFIDSLEKISNNNEIVSF*K**LQSKY**HINLLNFYF*FYPKGHIQGYACSML FKYMFNQDLSVESGMSRTIGNAVEHVFGNLSKLTAFDCFEIFSPLYDWFFTRRLKGCDIVRQIISS frame -2 DAK*KEQIQVFRNH*EFVNLKN*WKKKQLNLLIHLKKFQIIMKL*VFKNNNYNQNIDNILIY*IFIFSFIQKDIFKDMLVQCY SNICLIKIYQLKVACQELLVMQLNMFLVIFQN*LHLIVLKFSHHFMIGSLQED*KVAISLDK*SVV Frame -3 MPNEKSKSKCFAIIKSSST*KINGRKSN*IY*FT*KNFK***NCKFLKIIITIKILITY*FIKFLFLVLSKRTYSRICLFNVI QIYV*SRFIS*KWHVKNYW*CS*TCFW*SFKINCI*LF*NFLTTL*LVLYKKIKRLRYR*TNNQ*S JAX4a27h12.s1 Clone JAX4a27h12 Frame -1 KKKLKKKKKKNIALNLKFYIKKNGNN*CFFVSNHSFLGKRFCMYLNFYILENYYNLLILSIKKKKNKKKKVKKNKKIHTKSPS GPIAFPILGNVVQIRFWELFKIQEHELFGGYSKKYNGVVRAWFGERLFFFVSNYDVVKYFQKDENFHNRPSVLVPGWRYASSN GLGVMSSSDDKWKRAKSSVSLIKSSYN*KLKKKKQLNL*FT*KNFK***NWGILRV Frame -2 KKN*KKKKKKI*L*I*NFILRKMEIINVFLFLIILFLVKDFVCI*IFIF*KIITIY*FFP*KKKKIKKKRLKKIRKFIQNLQV DQLHFLFLEMLFKLDFGNYSKFKNMNYLVVIVKNIMVL*EHGLEKDYFFLFQIMML*SIFKKMKTSITDHQF*FQVGDMLQVM G*V**AHLMTNGKEQNQVFRSLRVRTTKN*RRKSN*IYDSLEKISNNNEIGGSLEW Frame -3 KKIKKKKKKKYSFKFKILY*EKWK*LMFFCF*SFFSW*KILYVFKFLYFRKLLQFINSFHKKKKK*KKKG*KK*ENSYKISKW TNCISYSWKCCSN*ILGIIQNSRT*IIWWL**KI*WCCKSMVWRKIIFFCFKL*CCKVFSKR*KLP*QTISFSSRLEICFK*W ARCNELI**QMEKSKIKCFAH*EFVQLKIKEEKAIEFMIHLKKFQIIMKLGDP*SG >JAX4a207g03.s1 Clone JAX4a207g03, KKKKKLCRKKKI*KKKKKKYSFKFKILY*EKWK*LMFFVSNHSFLGKNFVCI*IFIF*KIITFINSLHQKKKK*KKKG*KK*E NSYKISKWPIAFPILGNVVQIRFWELFKIQEHELFGGYSKKYNGVVRAWFGERLFFFVSNYDVVKYFQKDENFHNRPSVLVPG WRYASSNGLGVMSSSDDKWKRAKSSVSQSLRVRTTKKLMEEKARG JC2e75c06.s1 Clone JC2e75c06 Frame -3 FVCI*FYIF*K*LKLIN*FFTKKKKVKKIRKFIKISKWPIAFPILGNVVQIRFWELFKIQEHELIVRAWIGERLFLFVSIMM* SIFKKMKISFTNHPFSSRLEICFK*WIRCNELIDDEWKRAKSVFLIIKSSYIKKLMEEKALNLLIP*KNFE***NYRFFFKEI IIQLKLLILIYSPLF*VLSHPYSRICLFNVIQYMFNQDLSVG -2 FCMYLILYILEIIKIN*LILYKKKKGKKNKKIHQNLQVANCISYSWKCCSN*ILGIIQNSRT*INCKSMDWRKIIFICLNYDV KYFQKDENFLYKPSF*FQVGDMLQVMD*V**AH**RMEKSKISVFNN*EFIH*KINGRKSIEFIDSLKKFRIIMKL*VFF*RN NNSIKIININLFPIILSFIPPIFKDMLVQCYSIYV*SRFISW -1 ILYVFNFIYFRNN*N*LINSLQKKKR*KK*ENSSKSPSGQLHFLFLEMLFKLDFGNYSKFKNMN*L*EHGLEKDYFYLSQL*C KVFSKR*KFPLQTILLVPGWRYASSNGLGVMSSLMTNGKEQNQCF**LRVHTLKN*WKKKH*IY*FLEKISNNNEIIGFFLKK **FN*NY*Y*FIPHYFKFYPTHIQGYACSMLFNICLIKIYQL IFIFSFIQRTYSRICLFNVFKYMFIQDLSVESGMSRTIGNAVEHVFGNLS SEQUENCE 4 32% identical to CYP17s especially fish 17s C84082 Dictyostelium discoideum slug CLONE SSC732 IIIKLCNSPIHQEIAYNELKKVVSSKVIIDDSIKREITLSDRPNTPYIQAIIKETMRMHP VVVFGLPRYCENDIFIGDENYFIPKGCKVFINFHSIGYNEKYFKDPYKFEPNRFLENSNN SMDSFFPFGLGNRVCLGXQLANDQLYLVIANLILKYKLKTIDENKINEDGIFGLTVSPNK YKINLESR* C90646 Dictyostelium discoideum slug CLONE SSI265 IHQEIAYNELKKVVSSKVIIDDSIKREITLSDRPNTPYIQAIIKETMRMHPVVVFGLPRYCE NDIFIGDENYFIPKGCKVFINFHSIGYNEKYFKDPYKFEPNRFLENSXXXXXXXXX FGLGNRVCLGRQLANDQLYLVIANLILKYKLKTIDENKINEDGIFGLTVSPNKYKINLESR SEQUENCE 5 34% identical to CYP82 family C91402 Dictyostelium discoideum slug CLONE SSK171 LDKLLIEVENNRITQNALISICIDVLIAGTDTVGQTLSFAIVALVNNAEIQEKLSRNIID SMEKGDNHYSYSKYRNGIPYLALVVKEVFRMYPAGILGLPHMTSEDCEIQGHKIAKGTQI IQNIYSTHRSESFWPNPNNFIPERHIQNDVSKSVHFAVGTRNCMGMSLSEAEVHTAMAE LFGNFKFTNPSNIPLNDQGTFSVALNCPDFFVKIERRN* SEQUENCE 6 34% identical to CYP2, 17, 21 members C92981 Dictyostelium discoideum MISSING PERF REGION CLONE SSF410 Perf added in and N-terminal extension added on KVELLDQDNPKCFIDYLILQIRSDPSQTI (AU071546) KIDAIQYIVLDLLVGGTDSTSSALEWMILFLSNHESIQEKLYNEICKNTNTNTTATTTENDNES YFPKLIEKNNYPLFNACVKETLRRSPPVPLGLPHLCSEDTEIGGYLIPKGTQIISNIYSA SNCDKVFTDPLEFNPLRFIENS PPQTVTFSTGPRVCPGKNLSEDELFSFGTKLFKTFKFSRPNKNQLYDEVAILGITLEPKP FITKVSLRS* C91073 Dictyostelium CLONE SSJ454 EKNNYPLFNACVKETLRRSPPVPLGLPHLCSEDTEIGGYLIPKGTQIISNIYSASNCDKVF TDPLEFNPLRFIENSPPQTVTFSTGPRVCPGPNSSEDELFSFGTKLFKTFIFSRPNKNQ LYDEVAILGITLEPKPFITKVSLRS* AU037975 Dictyostelium discoideum CLONE SSH115 DLLVGGTDSTSSALEWMILFLSNHESIQXKLYNEICKNTNTTATTTENDNESYFPK LIEXNNYPLFNACVKETLRRSPPVPLGLPHLCSEDTEIGGYLIPKGTQIISNIYSA PPQTVTFSTGPRVCPGKNLSEDELFSFGTKLFKTFKFSRPN KNQLYDEVAILGITLEPKPFITKVSLR CLONE SSB769 KVELLDQDNPKCFIDYLILQIRSDPSQTIKIDAIQYIVLDLLVGGTDSTSSALEWMIL FLSNHESIQEKLYNEICKNTNTNTTATTTENDNESYFPKLIEKNNYPLFNACV SEQUENCE 7 36% identical to rabbit 2C1 40% with 93C1 C90627 Dictyostelium CLONE SSI868 DIISAGTDTSANTLVMALIELINNQEIQSKAFSSIRSSCLNDSNDDDDDDEIVITHSKYR SLLPYISMIIKETFRKHPIALLGLPHVTTEDVEIDGYKIEAGTYIIQNIFSSHRSDKIFQ SPNEFIPERFFESSQNQGLIHFGLGVRDCVGKSLAECEIFTLIATLLNRYQFINPNNSKK LNDIGTFGLAQVCPDTNIILKKRI* SEQUENCE 8 33% with 83D1 34% with 81D3 C90256 Dictyostelium CLONE SSI504 QSLENGEISWDSVVST CIDLQTAGADTSANTILYCLLELINNPNIQSKVYDDIKQAIIQSKENENQNDNENQEQTE EIITLSFNKYRTLAPYLSMVVKETFRKYPSGTIGLPHVTSEDVELNGYKICAGTQIIQNI WATHRNEKQFSEPDSFIPERFISQQQSANSNLIHFGCGVRDCIGKSLADSEIFTMLASLI NRYEFTNPNPSTPLNEIGKFGITYSCPENKIIIKKRF* C91045 CLONE SSJ415 NQNDNENQEQTEEIITLSFNKYRTLAPYLSMVVKETFRKYPSGTIGLPHV TSEDVELNGYKICAGTQIIQNIWATHRNEKQFSEPDSFIPERFISQQQSA NSNLIHFXCGVRDCIGKSLADSEIFTMLASLINRYEFTNPNPSTPLNEIG KFGITYSCPENKIIIKKRF* AU039107 Dictyostelium discoideum CLONE SSM394 SFNKYRTLAPYL SMVVKETFRKYPSGTIGLPHVTSEDVELNGYKICAGTQIIQNIWATHRNEKQFSEPDSFI PERFISQQQSANSNLIHFGCGVRDCIGKSLADSEIFTMLASLINRYEFTNPNPSTPLNEI GKFGITYSCPENKIIIKKRF AU039893 Dictyostelium discoideum 3 PRIME END OF CLONE SLG684 ENENQHDNENQEQTEEIITLSFNKYRTLAPYLSMVVKETFRKYPSGTIGLPHVTSEDVEL NGYKICAGTQIIQNIWATHRNEKQFSEPDSFIPERFISQQQSANSNLIHFGCGVRDCIGK SLADSEIFTMLASLINRYEFTNPNPSTPLNEIGKFGITYSCPENKIIIKKRF 5 PRIME END OF CLONE SLG684 MNYLVGLVLIFTIFYFFLQKNDKNMNSKIPGPKGIPILGNLLSMKGDLHLKLQEWYKQYG VIYRIKMGNVETVVLTEYPIIREAFIGNSNSFVNRFQRKSRLKLNNGENLVIVNGDIHNK LKTLVLSEMTNQRIKKYETSFIDNEIKKLFKVLDEHADTGKPIILNNHIKMFSMNIVLCF TFGLNYSYPYDEFEKASEFI SEQUENCE 9 43% identical to CYP17 sequences C90082 Dictyostelium discoideum CLONE SSG513 AMDPTVWEDPETFNPYRWLSSDISQSTYSFIPFGCGSRVCVGSSLARDEIFLGIGNILLNYIFES QNGKPINEKGHFGIALQTVDYNVKLTKI* SEQUENCE 10 34% identical with CYP2B members 33% identical to CYP17 members C25607 Dictyostelium discoideum CLONE SSA260 YTIPKGTQVMMSVYGMASDERYWKDPHIFNPERWLSSNHSTENGGGGGGVVGNSSQSEVF IPFGVGPRMCVGMGVAKDELYYCASQMFMNFKWSPVNDKPIDDEGVARIALEYKEYQVVL ERRK* SEQUENCE 11 42% identical to CYP81D6 C93191 Dictyostelium discoideum CLONE SSM757 5 PRIME AND 3 PRIME READS ADDED ILFSISDIIFAAVDSTSNGFSLLIGQLINKPEIQDK IYEEIMRNDENNNTNNISFADHTKYPYIISVMNESYRYNSSVPITEPNKTTEDVEVNGYKIA KGTMIIKNLRGTHLSKEFWGEDALEFKPERFKNQPLNQKGLFHFGAGARACPGGRFTESF TFTFLVIMLKNFKIVNPTDIPIDVEGEVGLAMQCKPFDALFIKRN* SEQUENCE 12 37% identical to CYP83 C84123 Dictyostelium CLONE SSC825 N TERMINAL OF SSC825 MESIINLIFYIIIFLILIDFLKKNISFRKNEPPRGGVAFPIFGD LPKLGENPHRYLTNLAMKKGGIYSVWLGDEKVFILTDPEAVRDAWVKQFRNFSDHPKTKS VRIFSGNFNDMAFAEYSQWKINSKWVSSAFTKTKLKTIGDLIEKESNYFIEHLKAYSNSG QPIFPKPYISKFGINVISGMMFSQVISKDESVDKGA C-terminal of clone SSC825 LDKVSIPYSVGNRKCPGASLSELALFSLCSNILLNFELKSIDGKPIDDTEVYGLTIHTKVHPISLTLRP* AU039172 Dictyostelium discoideum CLONE SSM473 PVGVLGIPRVALEETTIMGYTIPKGSQIFQNVYGMSHLFVSDPYKFKPERWIEYKLDKVSIPYSVGNRKCP GASLSELALFSLCSNILLNFELKSIDGKPIDDTEVYGLTIHTKVHPISLTLRP* AU037246 Dictyostelium discoideum CLONE SSB654 DQLVYKVFPRVALEEKTIMGYTIPKGSQIFQNVYGMSXLLESDPXKFKPERWIEYK XDKVSIPYSVGNRKCPGASLXELA*FSLCSNIFLNFELKSIDGKPIDDTEVYGXTIHTK SEQUENCE 13 55% identical to 2B19 (short region) C90134 CLONE SSI130 DGKLDPTNDLALISLPFSSGIRNCPGVGFAEYELFLLFSNIILNFHLSSP NNLKLNESGHFGLTMKPFPFLVDL C84168 Dictyostelium discoideum slug cDNA, clone SSC405 GIRNCPGVGFAEYELFLLFSNIILNFHLSSPNNLKLNESGHFGLTMKPFPFLV N-terminal half of SSC405 starting before the C-helix NNFTDRPQTKVFEIISRNYKNSGFANGEKWKHLRGLYAPSFTKIKSRPHENIILKYVNFE IKSLKNHAITNSIYNPFLIENINSFGTKVITEIIFGREFSENEVYSLIG PMNKLFGILDT PFPSESISFLKPFYRRSYKECDKQCEELFKLVEKVYDDHLLNLDKDNPKDVMDVMIVETD FKEKDHVICICCDLLMGTKDTFNTIVLWFFVLMINYQDVQLKGYQEIIK CLONE SSK320 NTERM (NOT IN GENBANK) MILLTLLYLIIFYIIIDFIKKNYKTKNQLPSPLGIALPIIGHLHLLRTDPYKTLA KASKKTEHGILKCWNGEHLMVVVDNPSIIKQMYVNTNNFTDRPQTKVFEIISRNY KNSGFANGEKWKHLRGLYAPSFTKIKSRPHENIILKYVNFEIKSLKNHAITNSIYN PFLIENINSFGTKVITEIIFGREFSENEVYSLIG CONSENSUS OF SEQ. 13 MILLTLLYLIIFYIIIDFIKKNYKTKNQLPSPLGIALPIIGHLHLLRTDPYKTLA KASKKTEHGILKCWNGEHLMVVVDNPSIIKQMYVNTNNFTDRPQTKVFEIISRNY KNSGFANGEKWKHLRGLYAPSFTKIKSRPHENIILKYVNFEIKSLKNHAITNSIYN PFLIENINSFGTKVITEIIFGREFSENEVYSLIGPMNKLFGILDTPFPSESISFLKPFYR RSYKECDKQCEELFKLVEKVYDDHLLNLDKDNPKDVMDVMIVETDFKEKDHVICI CCDLLMGTKDTFNTIVLWFFVLMINYQDVQLKGYQEIIK (GAP) DGKLDPTNDLALISLPFSSGIRNCPGVGFAEYELFLLFSNIILNFHLSSP NNLKLNESGHFGLTMKPFPFLVDL SEQUENCE 14 54% identical to CYP23A1 (C. elegans) 60% with 34A1 (C. elegans) short region C90413 CLONE SSI404 N-TERMINAL OF CLONE SSI404 PTPKLALPLIGHLYLLGDRPNRSFLELSKRYGGIFKIWMGEYPTVVLTDPDHVNEVWCKQ FLNFTNRPHFNSLDQFSSGFRNLSFSDYPLWSELRKLVSSSFTKSKVKGISNLLETQTNY LINTMNNYSINNKPFNPKKYIHKLTLNVVCMIAFSKEIKNDEDVNEGDMARLTKPKEM C-TERMINAL OF CLONE SSI404 KHKYYNDLDKISIPFSTGRRGCVGVQLGEAELYIVCANLV YNFKIESWDGKKINELEDFGIIIHPSSHNLKITKRNNK* SEQUENCE 15 57% identical to C92049 40% identical to fish CYP17 AU038895 Dictyostelium discoideum SS (H.Urushihara)CLONE SSM113 N-TERMINAL PART OF CLONE SSM113 AQRFTLSIMFKYIFNEDISYDEDITKGKIAELVQPMDQIFKSLGSGKLGDFISIAQPFYY QWLKFSDKQFNGPFSTVKKFIYKRYLEHINTIDHDNPRDLMDLLI C-TERMINAL OF CLONE SSM113 INEFSNDKNLIPTILQTSLDMFLAGN SASIIWFVLRMQEHPEIQLKAYNEIKDAVGDRDRVLLSDRPKTPYLNAIIKEILRLNPVG PFGLPHRSSNDIVIGNGKYFIPKDSQILVNYRGLGFNEKYFENPSQFDPSRFLNKNNDA YMPFGVGDRKCVGLQLAGDEQYLSFSNILLNFNLKNIGTPVSDYEEFGLTLKPNKFKV LLESRK* SEQUENCE 17 41% to Y10491 from soybean AU037557 Dictyostelium discoideum CLONE SSD784 LRMRTPAPLSLIRVSEEDFMTSGGIFIPKGTQIVPNLYGIGQNFVDDPSSYKPERWVE NDLDKVVLPFSIGPRNCPGNIISEINLFLACSN ILLNFEFSNGGKKIDETEVFGITIHPKDFSIQLKKRE* SEQUENCE 18 38% identical to A. bisporus Z82021 (mushroom) AU036927 Dictyostelium discoideum SS (H.Urushihara) Dictyostelium N-TERMINAL PART OF CLONE SSB828 IFHKSFDKFYDKYKDFYFIKFGQHDCIVLXSPKLIKQVVIEQSDSVFERFHTPSIKRYAQ GKSILGCSPDEWKKLRSFIVISFSKNKMGQQVLDKIFHTQYLXFENHIKKLIKSNNNIVT LEPEFKRLTISIIFNFQFGTDLEFTDPLIDSLLVCTEKIIASCQKASDLMPIFEIFTSYK DIDGVVKEMY C-TERMINAL PART OF CLONE SSB828 AKGESYTIPVGTLIIPNAHSFNFDPQYHKDPLTFNPNRYIGDNPEILHMTFDIGIRTCPFMSFAIDE LFIIFSRLFQSFEFQPIDNTPISEEAFTINSIRPKQWSCQVIERDHK* SEQUENCE 19 complete sequence This is the Dicty CYP51 sequence. 41% to S. bicolor CYP51 AU033519 Dictyostelium discoideum CLONE SLB124 MIGTIAVLVIAILVIFAFKKSPSNIPPIVETIPFIGCFYQFAKNPLQLVRNSYDRLGEIF TLHLMGFKMTFVLGPEAQALFFRGTDEELSPKEAYRFVTPVFGKGVVYDSETEIMY EQLRFVKNGLVLSQLKKAVGIIQEETEKYFETKWGDSGEIDLLYEMNKLTILTASRCLMGKS INKSLGQSGQLADLYPNWKK VSIQFHSFSQISHYHHSKRETLPVLKSRHFPSIIQERRRSTDDSVDDVLYTLMNSKYKDGSVLEDEQIVGLM IGLLFAGQHTSSITLTYTI FYLLNNLEYFDETQKDINDIVQKENQGEINFDGLKRMNRLETVIREVLRLHPPLIFLMRKVMTPMEYK GKTIPAGHILAVSPQVGMRLPTVYKNPDSFEPKRFDVEDKTPFSFIAFGGGKHGCPGENF GILQIKTIWTVLSTKYNLEVGPVPPTDFTSLVAGPKGPCMVKYSKKQK* SEQUENCE 20 not in genbank yet from Tsukuba 33% identical to mammalian CYP17s FC-BG13 I-HELIX TO END GGKEEQSSIIPMVNELILAGTETNSSTAEWFILTMVNNLDYQDKIYNELKSTLETTT AMIKLSNRNQTPLFNAALKEVLRLYPPVPFGVPRQVNQSFEINGGSLKIPKGTQIIQSLY SIFRDENYWDSPDQFKPERFLDQDSHSNNYFPYGIGVRNCIGMGFSQDELYISLSNLVLN FKLLPLIENSKICDKPIFGFSFKPNEFKINLEKRNN*