P450s that have appeared since the 1993 P450 nomenclature update.
Part C covering CYP10 to CYP69
Animal P450 names continue at CYP301 in this file
Lower eukaryote names continue at CYP501-699 in this file
Lower eukaryote names have used up all 3 digit names and now
Continue at CYP5001.
ALL Drosophila melanogaster P450S ARE NOW INCLUDED
This includes list references that were incomplete and duplications of
sequences that were already in the update. If a sequence is assigned an
accession number that was not in the old update it is included in this list.
Some expressed sequence tags (ESTs) are also included from humans.
This list last revised Aug. 24, 2009 (adding in all new fungal CYP names)
This list was last revised on Dec. 31, 2007.
Added all Neurospora P450s see 527A1-553A1
Added CYP51 Trypanosoma brucei
Added Fugu P450s, added CYP505A3 added CYP18 Spodoptera
Added all human genes and pseudogenes
Added 104 Anopheles genes
Revised May 13, 2003 named all remaining Dictyostelium P450 genes
Many sequences have been added without updating this list (May 11, 2005)
Added names for Nectria haematococca (158 genes/pseudogenes) Feb. 16, 2006
Added all Phanerochaete chrysosporium (white rot fungi) genes Aug. 29, 2007
Compiled by David R. Nelson
There are 80 C. elegans P450s listed here, surpassing the known mouse and human
complements. The C. elegans genome is now about 90% finished sequence and 9%
unfinished (one strand or one chemistry). About 1Mb is not cloned at three
telomeres and two internal sites. A special issue of Science appeared in Dec. 98 even though the genome is not completely done. The amount of sequence in the Blast searchable database at Washington Univ. is 140Mb, more than the 100Mb size of the genome. Therefore, we can guess that this set includes all the P450 genes in C. elegans, but the distrubution is not even. Most P450 genes (43 genes) are on chromosome V. see additional info on C. elegans P450s see this list. To see the actual sequences go to the C. elegans sequence file. So far we are missing CYP11A, CYP11B, CYP17, CYP19, CYP21, CYP24 and CYP27A and CYP27B. Does C. elegans make steroids? The present evidence would suggest not. Does C. elegans have
mitochondrial P450s? There is one probable mitochondrial P450 in C. elegans on
cosmid ZK177 named CYP44. It was thought to be incomplete but now a full length sequence
has been assembled from the genomic sequence.
10A Subfamily
11A Subfamily
11B Subfamily
12A Subfamily
12B Subfamily
13A Subfamily
13B Subfamily
14A Subfamily
16A Subfamily
17A Subfamily
18A Subfamily
19A Subfamily
21A Subfamily
22A Subfamily
23A Subfamily
24A Subfamily
25A Subfamily
26A Subfamily
27A Subfamily
27B Subfamily
28A Subfamily
29A Subfamily
30A Subfamily
31A Subfamily
32A Subfamily
33A Subfamily
33B Subfamily
33C Subfamily
33D Subfamily
33E Subfamily
34A Subfamily
35A Subfamily
35B Subfamily
35C Subfamily
35D Subfamily
36A Subfamily
37A Subfamily
37B Subfamily
40A Subfamily
41A Subfamily
42A Subfamily
43A Subfamily
44A Subfamily
45A Subfamily
46A Subfamily
51A Subfamily
52A Subfamily
52B Subfamily
52C Subfamily
52D Subfamily
52E Subfamily
52F Subfamily
53A Subfamily
53B Subfamily
54A Subfamily
55A Subfamily
56A Subfamily
57A Subfamily
58A Subfamily
59A Subfamily
60A Subfamily
60B Subfamily
61A Subfamily
62A Subfamily
63A Subfamily
64A Subfamily
65A Subfamily
66A Subfamily
67A Subfamily
10A Subfamily
CYP10A1 Lymnaea stagnalis (pond snail)
GenEMBL S46130 (1870bp) PIR JX0225 (545 amino acids)
AAB23599.1
Teunissen,Y., Geraerts,W.P., van Heerikhuizen,H., Planta,R.J.
and Joosse,J.
Molecular cloning of a cDNA encoding a member of a novel
cytochrome P450 family in the mollusc Lymnaea stagnalis.
J. Biochem. 112, 249-252 (1992)
Mitochondrial clan
1 MAIMKKFIHH SLKQLIKPNL TSTKRVVSTS PRKEQGVAAI SLEPSEMAQC PFRKSIDTFT
61 ETTNAVKAPG MTEVQPFERI PGPKGLPIVG TLFDYFKKDG PKFSKMFEVY RQRALEFGNI
121 YYEKVGHFHC VVISSPGEYS RLVHAERQYP NRREMVPIAY YRKQKGFDLG VVNSQGEEWY
181 RQRTVVSKKM LKLAEVSNFS TQMGEVSDDF VKRLSHVRDS HGEIPALERE LFKWAMESIG
241 TFLFEERIGC LGQETSPMAQ TFIANLEGFF KTLQPLMYNL PTYKLWSTKL WKQFENYSDN
301 VIDIGRSLVE KKWHPCKMEV TQNLHLISYL VNNGSMSTKE VTGLIVDLML AAVETTSSAT
361 VWCLYNLAKN PQVQEKLFQE ITEAQAKNNG TISAEDLCKL PMVKAVVKET LRLYPITYST
421 SRNIAEDMEL GGYTIPAGTH VQANLYGMYR DPSLFPEPEG ILPERWLRMN GSQMDATIKS
481 TSQLVWGHGA RMCLGRRIAE QEMHITLSKI IQNFTLSYNH DDVEPILNTM LTPDRPVRIE
541 FKPRQ
CYP10A2 Biomphalaria glabrata (bloodfluke planorb, carries schistosomiasis)
ESTs EV821686, EV821681, EV817279
Probable mitochondrial P450
LFDYFKKDGPRFNKI
FQVQQQRSAQYGEIYYEKIADFESVIESSPEEYNRLERSEGKYPCRREMA
PMAYYNRKKGYALGIVNSQGKDWYNLRTVVSKKMLKLSEVS
YGMFHNPDYFPEPETFLPERWLKENKMDATIKSASQLVWGHGARMCLGRRLAEQELHI
TIAKIIENFQLNYHGDEVQPILNTTMVPDRPVNIQFSRRQISKDTVFNNTH*
CYP10A3 Lottia gigantea (owl limpet)
JGI Protein ID:233081
53% to CYP10A1
MSSLIPKTFAKIAVTALPEIATKRVVSTSVAHKQSARVIEVDPLTLGTC
PFRSTFEKVESIVNPIEPKVEPQELTKETGIKSFEHVPGPK
GLPILGSLLDYFKKDGLRFDK
MFEAFKVRSIEFGPVYK
ESIGPIDTVVISDPAEYAKVIRADGKFPNRKEMEPMAF
YREQKGIGLGLVNSQGEEWHRQRSAVIKKML
MLNEVQDFSQPMNVVANDFITHLSSSRDASGE
IQQLDKQIFKWAMESIGTFLFEERIGCLNEK
PTELAQEFIDTLQKYFRLMQQLMYNLPMYKVFRTKKWQEFEK
LSDRIMEVGREFVDKKIE
KLQNADPSTSQEKGAFLTHLISQKS
LSTSDVTSNAVDLLSAAVETTSNASLWCLYNLATN
PEAQQTMFNEINTVLPNKEDVTPQALSKLPYVKAVLK
ETFRKYPITYATSRFLPENLEVGGYNIPAG
THVQANLYGMYSD
AKIFPEPEKFKPERWLRESKMDSQT
KSLSNLIWGHGARMCIGRRFAEQEMHIMLTK
IIQNFKLEYHHEPVEPTLNT
VMTPDRPVQIKFVPRS
CYP10B1 Capitella capitata (Gallery worm, polychaete worm, annelid)
JGI Protein ID:143013
46% to CYP10A1
MKGGQVVRKEKKLKPFKDIPGPKG
LPVIGTLLEFTKKDGLKFNK
MFEVMTSRSKEFGPVYK
ERIGMIESVIVSDPHEYAKVIQVDGKHPHRIELFPMVH
YRQKKKMALGTVNAQGEEWYRGRMVLSKVML
KPKEVQDYVIAQSDVGNDFIRHMKSIVSSDGQ
LISFEREIFKWALESICVVLFEERIGCFPNP
PTEKAQEFITNLIGFFKYMQPLMYNFPMYKIYPTKTWRQYEK
HGDIVVGIG
LDMVRKVKKESKSEFLTYLLAQKS
LSPVEANSHAVDLMMGAVETTANSMMWMLYCLSNF
PDAQRKLQEEIDKIVPQDERITPEVLSKMKYTKACLK
ETFRLFPITFATSRMIHEEIELGGYHIPKG
THCQANLWGMGRD
PDYFSDPLTFKPERWLRDIQTLEHH
NPHAVLPFGHGARMCIGLRFAEQEIYIAVAK
LMKHFTVKHEGELLPVLNT
VMTPDRPVNFRFLLRTKPALSGLGDTKKPIAVELK
CYP10B2 Capitella capitata (Gallery worm, polychaete worm, annelid)
JGI Protein ID:134490
87% to CYP10B1, 47% to CYP10A1
KLKPFKDTPGPK
GLPVIGTLLEFTKKDGLKFNK
MFEVMTSRSKEFGSVYK
ERIGFLESVIVSDPDEYTKVIKVDGKYPHRIEMLPMVH
YRQKKKMALGTVNAQGEEWYRARMVLSKVML
KPKEVQEYVIAQSDVGNDFIKHMKSIASNDGQLLN
FEREIFKWALESICVVLFEERIGCFPNP
PTERAQEFIANLIGFFKYMQPLMYNLPVYKIYPTKTWRKYEK
HGDTVFGIGLDMV
TKVFLPGEKESKSEFLTYLLAQKS
LSPVEANSHAVDLMIGAVETTANSMMWMLYCLSNF
PEVQRKLQDEIDATLPQDEHITPEILSRLKYTRACLK
ETFRLYPVTFATSRMIHKDIELGGYHIPKG
THCQANLWGMGRD
PDYFSDPLTFKPERWQRDTQTLEYH
NPHAVLPFGHGARMCIGRRFAEQEIYIAVAK
LMKHFTVEHVGHLHPILIT
VMIPDRPVNFRFRPRTKPER
CYP10C1 Helobdella robusta (leech, annelid worm)
JGI Protein ID:67702
48% to CYP10B2, 41% to CYP10A1
MRKYIIQIISRNKIHNFNNYDRIGNNILKV
NCVSTSAAAFDNTDLLVSNANVLTENKLAELKSFESLPGPK
GLPLFGTLLSYVRPGGLSFRK
MFKVMDMRAKQYGSVYK
ETIGSNLTSVIVSDMEEYLKVIRADGKMPNRIELEPFKY
FRERNNFDIGIVNSQGLQWLEKRQILGPKIM
ALSEILDHVGPINDVTMDFLKRMDEKLLDRDGLFK
NLERNLFTWALEAVGTYLFEARIGCLNEP
IPPFADMFITHLSGMFREMQGLMYGPPIYKYISTRNWKRFED
HCYKIM
EAGMTLIEETSSSSALLTFLLSQKS
MSASSVNLTSIDVMIGAIETTSNSFTWMLYCLARN
PEVQEKLFEEVLQYLPNKDSPVTREVIRKMDYVRACLK
ETLRFYPITFATSRKLASEIELKGFRIPKG
THVQANLWTIGRD
ERLFPHPEKFNPDRWLRKSHEVSHLA
TALSHLPFGHGPRMCIGQRLAEMEIYTMVSK
LIRNFKVECAEKGEVQPILMT
VLTPDRPMMLKFKRR
11A Subfamily
CYP11A1 human
PIR A48733 (239 amino acids)
Matteson, K.J., Chung, B.C., Urdea, M.S. and Miller, W.L.
Study of cholesterol side-chain cleavage (20,22 desmolase)
deficiency causing congenital lipoid adrenal hyperplasia
using bovine-sequence P450scc oligodeoxyribonucleotide
probes.
Endocrinology 118, 1296-1305 (1986)
CYP11A1 human
GenEMBL M14565
Chung BC, Matteson KJ, Voutilainen R, Mohandas TK, Miller WL. (1986)
Human cholesterol side-chain cleavage enzyme, P450scc: cDNA cloning,
assignment of the gene to chromosome 15, and expression in the
placenta. Proc Natl Acad Sci U S A. 83, 8962-8966.
First complete human CYP11A1 cDNA
CYP11A1 rabbit
GenEMBL S59219 (1336bp) PIR A49189 (445 amino acids)
Yang,X., Iwamoto,K., Wang,M., Artwhol,J., Mason,J.I. and
Pang,S.
Inherited congenital adrenal hyperplasia in the rabbit is caused by
a deletion in the gene encoding cytochrome P450 cholesterol side
chain cleavage enzyme.
Endocrinol. 132, 1977-1982 (1993)
CYP11A1 bovine
PIR A42033 (18 amino acids)
Pikuleva, I.A., Lapko, A.G., Chashchin, V.L.
Functional reconstitution of cytochrome P-450-scc with hemin
activated with Woodward's reagent K. Formation of a
hemeprotein cross-link.
J. Biol. Chem. 267, 1438-1442 (1992)
CYP11A1 Bos taurus (cow)
See cattle page for details
MLARGLPLRSALVKACPPI
LSTVGEGWGHHRVGTGEGAGISTKTPRPYSEIPSPGDNGWLNLYHFWREKGSQRIHFRHI
ENFQKYGPIYREKLGNLESVYIIHPEDVAHLFKFEGSYPERYDIPPWLAYHRYYQKPIGV
LFKKSGTWKKDRVVLNTEVMAPEAIKNFIPLLNPVSQDFVSLLHKRIKQQGSGKFVGDIK
EDLFHFAFESITNVMFGERLGMLEETVNPEAQKFIDAVYKMFHTSVPLLNVPPELYRLFR
TKTWRDHVAAWDTIFNKAEKYTEIFYQDLRRKTEFRNYPGILYCLLKSEKMLLEDVKANI
TEMLAGGVNTTSMTLQWHLYEMARSLNVQEMLREEVLNARRQAEGDISKMLQMVPLLKAS
IKETLRLHPISVTLQRYPESDLVLQDYLIPAKTLVQVAIYAMGRDPAFFSSPDKFDPTRW
LSKDKDLIHFRNLGFGWGVRQCVGRRIAELEMTLFLIHILENFKVEMQHIGDVDTIFNLI
LTPDKPIFLVFRPFNQDPPQA*
CYP11A1 Sus scrofa (pig)
GenEMBL X13768 (see also NM_214427)
Mulheron GW, Stone RT, Miller WL, Wise T. (1989)
Nucleotide sequence of cytochrome P-450 cholesterol side-chain
cleavage cDNA isolated from porcine testis.
Nucleic Acids Res. 17, 1773
CYP11A1 Sus scrofa (pig)
GenEMBL L34259 (2376bp), NM_214427
Urban,R.J., Shupnik,M.A. and Bodenburg,Y.H.
Insulin-like growth factor-I increases expression of the porcine
P-450 cholesterol side chain cleavage gene through a GC-rich domain.
J. Biol. Chem. 269, 25761-25769 (1994)
MLARGLALRSVLVKGCQPFLSAPRECPGHPRVGTGEGACISTKT
PRPFSEIPSPGDNGWINLYRFWKEKGTQKIHYHHVQNFQKYGPIYREKLGNLESVYII
DPEDVALLFKFEGPNPERYNIPPWVAYHQHYQKPVGVLLKKSGAWKKDRLVLNTEVMA
PEAIKNFIPLLDTVSQDFVGVLHRRIKQQGSGKFSGDIREDLFRFAFESITNVIFGER
LGMLEEIVDPEAQKFIDAVYQMFHTSVPMLNLPPDLFRLFRTKTWRDHVAAWDTIFNK
AEKYTQNFYWDLRRKREFNNYPGILYRLLGNDKLLSEDVKANVTEMLAGGVDTTSMTL
QWHLYEMARSLNVQEMLREEVLNARRQAQGDTSKMLQLVPLLKASIKETLRLHPISVT
LQRYLVNDLVLRDYMIPAKTLVQVAVYAMGRDPAFFSNPGQFDPTRWLGKERDLIHFR
NLGFGWGVRQCVGRRIAELEMTLFLIHILENFKVELQHFSDVDTIFNLILMPDKPIFL
VFRPFNQDPLQA
CYP11A1 goat
GenEMBL D50058 (1825bp)
Okuyama,E., Okazaki,T., Furukawa,A., Wu,R.-F. and Ichikawa,Y.
Molecular cloning and nucleotide sequences of cDNA clones of sheep
and goat
adrenolcortical cytochrome P450scc.
unpublished (1995)
CYP11A1 sheep
GenEMBL D50057 (1825bp)
Okuyama,E., Okazaki,T., Furukawa,A., Wu,R.-F. and Ichikawa,Y.
Molecular cloning and nucleotide sequences of cDNA clones of sheep
and goat
adrenolcortical cytochrome P450scc.
unpublished (1995)
CYP11A1 bovine
PIR S29644 (21 amino acids)
Tsujita, M. and Ichikawa, Y.
Substrate-binding region of cytochrome P-450(scc) (P-450
XIA1). Identification and primary structure of the
cholesterol binding region in cytochrome P-450(scc).
Biochim. Biophys. Acta 1161, 124-130 (1993)
Cyp11a1 mouse
GenEMBL J05511, NM_019779, AF195119
Rice,D.A., Kirkman,M.S., Aitkin,L.D., Mouw,A.R., Schimmer,B.P. and
Parker,K.L.
Analysis of the promoter region of the gene encoding mouse
cholesterol side-chain cleavage enzyme
J. Biol. Chem. 265, 11713-11720 (1990)
MLAKGLSLRSVLVKGCQPFLSPTWQGPVLSTGKGAGTSTSSPRS
FNEIPSPGDNGWLNLYHFWRESGTQKIHYHQMQSFQKYGPIYREKLGTLESVYIVDPK
DASILFSCEGPNPERFLVPPWVAYHQYYQRPIGVLFKSSDAWKKDRIVLNQEVMAPGA
IKNFVPLLEGVAQDFIKVLHRRIKQQNSGNFSGVISDDLFRFSFESISSVIFGERMGM
LEEIVDPEAQRFINAVYQMFHTSVPMLNLPPDFFRLLRTKTWKDHAAAWDVIFNKADE
YTQNFYWDLRQKRDFSQYPGVLYSLLGGNKLPFKNIQANITEMLAGGVDTTSMTLQWN
LYEMAHNLKVQEMLRAEVLAARRQAQGDMAKMVQLVPLLKASIKETLRLHPISVTLQR
YTVNDLVLRNYKIPAKTLVQVASFAMGRDPGFFPNPNKFDPTRWLEKSQNTTHFRYLG
FGWGVRQCLGRRIAELEMTILLINLLENFRIEVQNLRDVGTKFSLILMPENPILFNFQ
PLKQDLGPAVTRKDNTVN
CYP11A1 Xenopus tropicalis
See Xenopus pages for seq
CYP11A1 Danio rerio (zebrafish)
See xebrafish pages for seq
CYP11A1 Oncorhynchus mykiss (rainbow trout)
GenEMBL S57305 (1789bp) Swiss Q07217 (514 amino acids)
PIR S32197 (514 amino acids)
Takahashi,M., Tanaka,M., Sakai,N., Adachi,S., Miller,W.L.
and Nagahama,Y.
Rainbow trout ovarian cholesterol side-chain cleavage
cytochrome P450 (P450scc). cDNA cloning and mRNA expression
during oogenesis.
FEBS Lett. 319, 45-48 (1993)
CYP11A1 Fugu rubripes (pufferfish)
No accession number
Scaffold_1630
37515 MARWSVWRSPVVLPLSRMEVPMTGARHSSTMPVARQTYSDSSSFV 37381
37380 RSFNDIPGLWKNGVANLYNFWKLDGFRNLHHIMVQNFNTFGPIYR 37246 (2)
37065 EKIGYYESVNIINPEDAAILFKAEGHYPKRLKVEAWTSYRDYRNRKYGVLLK 36904 (2)
36822 NGEEWRCNRVLLNKEVISPKVLENFVPLLDEVGNDFVVRVHKKIARSGQNKWTTDLSQELFKYALES 36628 (1)
36506 VSSVLYGERLGLFLDYIDPEAQHFIDCISLMFKTTSPML
YIPPALLRKVGAKVWRDHVEAWDGIFNQ 36300 (1 expected) bad boundary
36208 ADRCIQNIYRRLRQETGPSKKYPGVLASLLLRDKLSIEDIKASITELMAGGVDT 36047 (0)
35975 TSITLLWTLYELARHPNLQEELRAEVAAARTESQGDMLEMLKRIPLVKGALKETLR 35808 (2)
35731 LHPVAVSLQRYIAEDIIIQNYHIPAG 35654 (0)
35568 TLVQLGLYAMGRDPKVFFRPEQYQPSRWLRSETHYFKSLGFGFGPRQCLGRRIAEAEMQLFLIH 35377 (0)
35297 MLENFRVEKQRHMEVQSTFELILLPDKPIILTLKPLSS* 35181
CYP11A1 Dasyatis americana (southern stingray)
GenEMBL U63299(4619bp)
Nunez,B.S. and Trant,J.M.
Isolation of the cDNA encoding the interrenal form of cholesterol
side chain cleavage cytochrome P450 of the southern stingray
(Dasyatis americana).
unpublished (1996)
CYP11A2 Danio rerio (zebrafish)
See xebrafish pages for seq
11B Subfamily
CYP11B1 human
GenEMBL J05140 M32863 (1482bp) M32878 (2633bp) M32879 (1155bp)
Mornet,E., Dupont,J., Vitek,A. and White,P.C.
Characterization of two genes encoding human steroid 11-beta-
hydroxylase (P-450-11-beta).
J. Biol. Chem. 264, 20961-20967 (1989)
CYP11B1 human
GenEMBL D16155 (156bp)
Naiki,Y., Shizuta,Y., Kawamoto,T., Yasushiro,M., Miyahara,K.,
Toda,K., Tadao,O. and Imura,H.
A nonsense mutation (TGG(116Arg)-TAG(stop)) in CYP11B1
causes steroid 11beta-hydroxylase deficiency.
J. Clin. Endocrinol. Metab. 77, 1677-1682 (1993)
CYP11B1 human
GenEMBL D10169 D90428 (2085bp)
Kawamoto,T., Mitsuuchi,Y., Toda,K., Yokoyama,Y., Miyahara,K.,
Miura,S., Ohnishi,T., Ichikawa,Y., Nakao,K., Imura,H., Ulick,S.
and Shizuta,Y.
Role of steroid 11beta-hydroxylase and steroid 18-hydroxylase in the
biosynthesis of glucocorticoids and mineralocorticoids in humans.
Proc. Natl. Acad. Sci. USA 89, 1458-1462 (1992)
CYP11B1 human
PIR S29068 (30 amino acids)
Kawamoto, T., Mitsuuchi, Y., Toda, K., Miyahara, K.,
Yokoyama, Y., Nakao, K., Hosoda, K., Yamamoto, Y., Imura,
H. and Shizuta, Y.
Cloning of cDNA and genomic DNA for human cytochrome P-450
(11-beta).
FEBS Lett. 269, 345-349 (1990)
CYP11B1 Papio hamadryas ursinus (chacma baboon)
GenEMBL U52085(228bp)
Hampf,M., Swart,A. and Swart,P.
Expression of Papio ursinus steroid 11beta hydroxylase
unpublished (1996)
CYP11B1 rat
GenEMBL D10107 (1528bp) S58847
PIR B46040 (499 amino acids)
Matsukawa,N., Nonaka,Y., Higaki,J., Nagano,M., Mikami,H.,
Ogihara,T. and Okamoto,M.
Dahl's salt-resistant normotensive rat has mutations in
cytochrome P450 (11 beta), but the salt-sensitive hypertensive
rat does not.
J. Biol. Chem. 268, 9117-9121 (1993)
Note: only 1 amino acid difference with 11B1 at position 84
E (normal) changed to G (seen in 11B2 and 11B3)
D10107 has five differences with D11354
CYP11B1 rat
GenEMBL S58858 (330bp) PIR JX0251(499 amino acids)
Nomura,M., Morohashi, K.-i., Kirita, S., Nonaka, Y.,
Okamoto,M., Nawata,H. and Omura,T.
Three forms of rat CYP11B genes: 11 beta-hydroxylase gene,
aldosterone synthase gene, and a novel gene.
J. Biochem. 113, 144-152 (1993)
Note: Fig. 3 has 6 errors. 1,2) aldo-46 amino acids 2 and 3 are
incorrectly translated gctctc = AL not HS. 3) 11B3 codon at 559-561
incorrectly translated gac = D not N. 4,5,6) 11beta-62, 11B1
and 11B3 codon at 964-966 tcc = Ser not Pro.
CYP11B1 rat
GenEMBL S58849, D14086 to D14091 PIR A46039 (499 amino acids)
Mukai,K., Imai,M., Shimada,H. and Ishimura,Y.
Isolation and characterization of rat CYP11B genes involved
in late steps of mineralo- and glucocorticoid syntheses.
J. Biol. Chem. 268, 9130-9137 (1993)
CYP11B1 rat
GenEMBL S63899 (596bp)
Mukai,K., Imai,M., Shimada,H., Okada,Y., Ogishima,T. and
Ishimura,Y.
Structural differences in 5'-flanking regions of rat cytochrome
P-450aldo and P-450(11) beta genes
Biochem. Biophys. Res. Commun. 180, 1187-1193 (1991)
CYP11B1 rat
GenEMBL D11354 (1528bp) PIR A46040 (499 amino acids)
Matsukawa,N., Nonaka,Y., Higaki,J., Nagano,M., Mikami,H.,
Ogihara,T. and Okamoto,M.
Dahl's salt-resistant normotensive rat has mutations in
cytochrome P450 (11 beta), but the salt-sensitive hypertensive
rat does not.
J. Biol. Chem. 268, 9117-9121 (1993)
Note: This sequence is the wild type DS rat sequence. The mutant
DR rat has five amino acids differences accession number D10107
CYP11B1 Cavia porcellus (guinea pig)
GenEMBL Z69785(2028bp)
Bulow,H.E., Mobius,K., Bahr,V. and Bernhardt,R.
Molecular cloning and functional expression of the cytochrome P450
11B-hydroxylase of the guinea pig.
Biochem. Biophys. Res. Commun. 221, 304-312 (1996)
CYP11B1 bovine
PIR JX0151 (503 amino acids)
Kirita, S., Hashimoto, T., Kitajima, M., Honda, S.,
Morohashi, K. and Omura, T.
Structural analysis of multiple bovine P-450(11beta) genes
and their promoter activities.
J. Biochem. 108, 1030-1041 (1990)
CYP11B1 Bos taurus (cow)
See cattle page for details
MALWAKARVRMAGPWLSLHEARLLGTRGAAAPKAVLPFEAMPRC
PGNKWMRMLQIWKEQSSENMHLDMHQTFQELGPIFRYDVGGRHMVFVMLPEDVERLQQ
ADSHHPQRMILEPWLAYRQARGHKCGVFLLNGPQWRLDRLRLNPDVLSLPALQKYTPL
VDGVARDFSQTLKARVLQNARGSLTLHIAPSVFRYTIEASTLVLYGERLGLLTQQPNP
DSLNFIHALEAMLKSTVQLMFVPRRLSRWMSTNMWREHFEAWDYIFQYANRAIQRIYQ
ELALGHPWHYSGIVAELLMRADMTLDTIKANTIDLTAGSVDTTAFPLLMTLFELARNP
EVQQAVRQESLVAEARISENPQRAITELPLLRAALKETLRLYPVGITLEREVSSDLVL
QNYHIPAGTLVKVLLYSLGRNPAVFARPESYHPQRWLDRQGSGSRFPHLAFGFGVRQC
LGRRVAEVEMLLLLHHVLKNFLVETLEQEDIKMVYRFILMPSTLPLFTFRAIQ
CYP11B1 pig
GenEMBL D38590(1671bp)
Sun,T., Zhao,Y., Nonaka,Y. and Okamoto,M.
Cloning and expression of cytochrome P450(11 beta) of porcine
adrenal cortex.
J. Steroid Biochem. Mol. Biol. 52, 227-232 (1995)
MAIWAKAEAWLAGPWLALNRARTLGTRAVLAPKGVLPFEAIPQF
PGKKWMRVLQLWREQGFENNHLEMHQTFQELGPIFRFDVGGRNMVLVMLPEDVERCQK
VEGLHPQRDVPGPWLAYRHLRGHKCGVFLLNGPTWRLDRLQLNPGVLSLQAMQKFTPL
VDGVARDFSQALRARVMQNARGSLTLDIKPSIFRYTIEASNLVLFGERLGLLAHQPNP
ESLDFIHALEVMFKSTVQLMFMPRSLSRWTSTGTWKEHFEAWDCIFQYANKAIQRLYQ
ELTLGHPWHYSGVVAELLTHANMTVDAIKANSIDLTAGSVDTTAYPLLMTLFELARNP
EVQQALRQESLAAAARISENPQKAITELPLLRAALKETLRLYPVGIFLDRCVTSDLVL
QNYHIPAGTLVKVLLYSLGRNPAVFARPERYHPQRWLDNQGSGTRFPHLAFGFGMRQC
LGRRLAQVEMLLLLHHVLKNFLVETLVQEDIKMIYRFIMTPSTLPLLTFRAIS
CYP11B1 sheep
GenEMBL L34337 (1639bp)
Boon,W.C., Roche,P.J., Hammond,V.E., Jeyaseelan,K.,
Crawford,R.J. and Coghlan,J.P.
Cloning and expression analysis of a cytochrome P450-11-beta
cDNA in sheep.
unpublished (1994)
CYP11B1 Ovis aries (sheep)
GenEMBL L28716 (2300bp)
Anwar,A., Jeyaseelan,K. and Coghlan,J.P.
Molecular cloning and characterization of the ovine CYP11B1
promoter.
Biochem. Mol. Biol. Int. 33, 1169-1178 (1994)
CYP11B1 Ovis ammon (sheep)
GenEMBL L47569(9027bp)
Anwar,A., Jeyaseelan,K. and Coghlan,J.P.
Characterization of an ovine 11-beta hydroxylase (cyp11b) gene.
unpublished (1995)
Cyp11b1 mouse
PIR A41552 (500 amino acids)
Domalik, L.J., Chaplin, D.D., Kirkman, M.S., Wu, R.C., Liu,
W., Howard, T.A., Seldin, M.F., Parker, K.L.
Different isozymes of mouse 11beta-hydroxylase produce
mineralocorticoids and glucocorticoids.
Mol. Endocrinol. 5, 1853-1861 (1991)
Cyp11b1 mouse
PIR A32210 (42 amino acids)
Mouw, A.R., Rice, D.A., Meade, J.C., Chua, S.C., White, P.C.,
Schimmer, B.P. and Parker, K.L.
Structural and functional analysis of the promoter region of
the gene encoding mouse steroid 11beta-hydroxylase.
J. Biol. Chem. 264, 1305-1309 (1989)
Cyp11b1 mouse
GenEMBL J04451
80% to mouse 11B2 64% to human 11B1 and 11B2 80% to 11B1 rat
78% to 11B2 rat. sequence below is from Ensembl mouse version 3
15.75000001-76000000 mouse chr 15 add 75 million to get global location
306415 MALRVTTDVWLARPWQCLHRTRALGTTATLAPKTLQPFEAIPQYSRNKWLKMIQILREQG 306356
306355 QENLHLEMHQVFRELGPIFR 306176 (?)
305773 HSVGKTQIVFVTLPEDVEKLYQVESTHPCRMPLESWIVHRELRGLGRGVFLL 305618 (?)
304234 NGPEWYFNRLQLNPNVLSPKAVQKFVPLVDGIARDFVDNLKKKMLESVHGSFSMDFQSSV 304175
304174 FNYTIE 304157 (?)
303918 ASHFVLFGERLGLIGRDLSPDSLKFLHTLHSMFKTTTQLLYLPRSLTRWTSTRVWKENLE 303859
303858 SWDFISEYG 303826 (?)
303446 TKCIKNVYRELAEGRPQSWSVTAELVAERTLSMDAIQANSMELIAGSTDT 303413 (0?)
301935 TSTPLVMTFFELARNPDVQQALRQESLAAEASIAANPQKAMSDLPLLRAALKETLR 301768
301535 LYPVGTFLERILSSDLVLQNYHVPAG 301458
301370 TVLNVNLYSMGRNPAVFPRPERYMPQRWLERKRSFKHLAFGFGVRQCLGRRLAEAEM 301314
301313 MLLLHH 301182 (?0)
300923 VLKSFHVETQEK 300888
300887 EDVRMAYRFVLMPSSSPLLTFRPVN 300705
CYP11B1 Xenopus tropicalis
See Xenopus pages for seq
CYP11B1 Danio rerio (zebrafish)
See xebrafish pages for seq
CYP11B2 human
GenEMBL J05140 M32864 (1809bp) M32880 (3088bp) M32881 (1101bp)
Mornet,E., Dupont,J., Vitek,A. and White,P.C.
Characterization of two genes encoding human steroid 11-beta-
hydroxylase (P-450-11-beta).
J. Biol. Chem. 264, 20961-20967 (1989)
CYP11B2 human
GenEMBL D90429 D10170 (2114bp)
Kawamoto,T., Mitsuuchi,Y., Toda,K., Yokoyama,Y., Miyahara,K.,
Miura,S., Ohnishi,T., Ichikawa,Y., Nakao,K., Imura,H., Ulick,S.
and Shizuta,Y.
Role of steroid 11beta-hydroxylase and steroid 18-hydroxylase in the
biosynthesis of glucocorticoids and mineralocorticoids in humans.
Proc. Natl. Acad. Sci. USA 89, 1458-1462 (1992)
CYP11B2 human
GenEMBL D13752(6910bp)
Kawamoto,T., Mitsuuchi,Y., Ohnishi,T., Ichikawa,Y., Yokoyama,Y.,
Sumimoto,H., Toda,K., Miyahara,K., Kuribayashi,I., Nakao,K.,
Hosoda,K., Yamamoto,Y., Imura,H. and Shizuta,Y.
Cloning and expression of a cDNA for human cytochrome P-450aldo as
related to primary aldosteronism.
Biochem. Biophys. Res. Commun. 173 (1), 309-316 (1990)
CYP11B2 human
GenEMBL S77398 S77401 S77403 S77406 S77409 (genomic sequences)
Shizuta,Y., Kawamoto,T., Mitsuuchi,Y., Miyahara,K., Rosler,A.,
Ulick,S. and Imura,H.
Inborn errors of aldosterone biosynthesis in humans.
Steroids 60 (1), 15-21 (1995)
CYP11B2 Bos taurus (cow)
See cattle page for details
MALWAKARVRMAGPWLSLHEARLLGTRGAAAPKAVLPFEAMPRCPGNKWMRMLQIWKE
QSSENMHLDMHQTFQELGPIFRYDVGGRHMVFVMLPEDVERLQQADSHHPQRMILEPWLA
YRQARGHKCGVFLLNGPQWRLDRLRLNPDVLSLPALQKYTPLVDGVARDFSQTLKARVLQ
NARGSLTLDIAPSVFRYTIEASTLVLYGERLGLLTQQPNPDSLNFIHALEAMLKSTVQLM
FVPRRLSRWMSTNMWREHFEAWDYIFQYANRAIQRIYQELALGHPWHYSGIVAELLMRAD
MTLDTIKANTIDLTAGSVDTTAFPLLMTLFELARNPEVQQAVRQESLVAEARISENPQRA
ITELPLLRAALKETLRLYPVGITLEREVSSDLVLQNYHIPAGTLVKVLLYSLGRNPAVFA
RPESYHPQRWLDRQGSGSRFPHLAFGFGVRQCLGRRVAEVEMLLLLHHVLKNFLVETLEQ
EDIKMVYRFILMPSTLPLFTFRAIQ*
CYP11B2 Sus scrofa (pig)
ESTs CV872108.1, BI341717.1
93% to 11B1 pig
MAIWAKAEAWLAGPWLALHRARTLGTRAALAPKAVLPFEAIPQCPGNKWMRVLQLWREQG
FENIHLDMHQTFQELGPIFRFDVGGRHMVLVMLPEDVERLQKVEGLHPQRMFLEPWLAYR
QLRGHKCGVFLLNGPTWRLDRLQLNPGVLSLQAMQKFTPLVDGVARDFSQALRARVMQNA
RGSLTLDIKPSIFRYTIEASNLVLFGERLGLLAHQPNPES
CYP11B2 rat
GenEMBL S58859 (353bp) PIR JX0252 (500 amino acids)
Nomura,M., Morohashi, K.-i., Kirita, S., Nonaka, Y.,
Okamoto,M., Nawata,H. and Omura,T.
Three forms of rat CYP11B genes: 11 beta-hydroxylase gene,
aldosterone synthase gene, and a novel gene.
J. Biochem. 113, 144-152 (1993)
CYP11B2 rat
GenEMBL S58850 D14092 to D14097
PIR B46039 (500 amino acids)
Mukai,K., Imai,M., Shimada,H. and Ishimura,Y.
Isolation and characterization of rat CYP11B genes involved
in late steps of mineralo- and glucocorticoid syntheses.
J. Biol. Chem. 268, 9130-9137 (1993)
CYP11B2 rat
GenEMBL S63898 (594bp)
Mukai,K., Imai,M., Shimada,H., Okada,Y., Ogishima,T. and Ishimura,Y.
Structural differences in 5'-flanking regions of rat cytochrome
P-450aldo and P-450(11) beta genes.
Biochem. Biophys. Res. Commun. 180, 1187-1193 (1991)
CYP11B2 rat
GenEMBL S64136 (3001bp) PIR JN0615 (506 amino acids)
GenEMBL U14908 (3000bp)
Zhou,M. and Gomez-Sanchez,C.E.
Cloning and expression of a rat cytochrome P-450 11
beta-hydroxylase/aldosterone synthase (CYP11B2) cDNA variant.
Biochem. Biophys. Res. Commun. 194, 112-117 (1993)
Erratum: Biochem. Biophys. Res. Commun. 196, 1018 (1993)
CYP11B2 rat
PIR A34281 (20 amino acids) Swiss P30099 (510 amino acids)
Ogishima, T., Mitani, F., Ishimura, Y.
Isolation of aldosterone synthase cytochrome P-450 from zona
glomerulosa mitochondria of rat adrenal cortex.
J. Biol. Chem. 264, 10935-10938 (1989)
Note: sequence is from N-terminal after signal sequence
CYP11B2 rat
Swiss P30099 (510 amino acids) GenEMBL D00567 (2824bp)
Swiss P30100 (500 amino acids) GenEMBL D00568 (2705bp)
Matsukawa,N., Nonaka,Y., Ying,Z., Higaki,J., Ogihara,T. and
Okamoto,M.
Molecular cloning and expression of cDNAs encoding rat aldosterone
synthase: variants of cytochrome P-450 11beta
Biochem. Biophys. Res. Commun. 169, 245-252 (1990)
CYP11B2 hamster
GenEMBL S73810 (1503bp)
LeHoux,J.G., Mason,J.I., Bernard,H., Ducharme,L., LeHoux,J.,
Veronneau,S. and Lefebvre,A.
The presence of two cytochrome P450 aldosterone synthase mRNAs in
the hamster adrenal.
J. Steroid Biochem. Mol. Biol. 49, 131-137 (1994)
CYP11B2 cavia porcellus (domestic guinea pig)
GenEMBL AF018569
Buelow,H.E. and Bernhardt,R.
Molecular Cloning and Functional Expression of the Guinea Pig
Aldosterone Synthase.
Unpublished
CYP11B2 Rana catesbeiana (bullfrog)
GenEMBL D10984 (1919bp)
Nonaka,Y., Takemori,H., Halder,S.K., Sun,T., Ohta,M., Hatano,O.,
Takakusa,A. and Okamoto,M.
Frog Cytochrome P-450 (11 beta, aldo), a single enzyme involved in
the final steps
of glucocorticoid and mineralocorticoid biosynthesis.
Eur. J. Biochem. 229, 249-256 (1995)
Cyp11b2 mouse
GenEMBL S85260 (2804bp)
Domalik,L.J., Chaplin,D.D., Kirkman,M.S., Wu,R.C., Liu,W.W.,
Howard,T.A., Seldin,M.F. and Parker,K.L.
Different isozymes of mouse 11 beta-hydroxylase produce
mineralocorticoids and glucocorticoids.
Mol. Endocrinol. 5, 1853-1861 (1991)
Cyp11b2 mouse
genEMBL S85260
68% to human 11B2 67% to human 11b1 94% to 11B2 rat 81% to 11B1 rat
sequence below is from Ensembl mouse version 3
15.75000001-76000000 mouse chr 15 add 75million to get global location
321034 MALRVTADVWLARPWQCLHRTRALGTTATLAPKTLQPFEAIPQYSRNKWLKMIQILREQG 320975
320974 QENLHLEMHQVFRELGPIFR 320795 (?)
320391 HSVGKTQIVSVMLPEDAEKLHQVESMLPRRMHLEPWVAHRELRGLRRGVFLL 320238 (?)
318803 NGPEWRLNRLRLNRNVLSPKAVQKFVPMVDMVARDFLETLKEKVLQNARGSLTM 318762
318761 DVQQSLFNYTIE 318606 (?)
318477 ASNFALFGERLGLLGHDLSPGSLKFIHALHSMFKSTS 318487
318486 QLLFLPKSLTRWTSTRVWKEHFDAWDVISEYG (?) 318391
318000 NRCIWKVHQELRLGSSQTYSGIVAELISQGSLPLDAIKA 318004
318003 NSMELTAGSVDT 317848 (?)
316981 TAIPLVMTLFELARNPDVQKALRQESLAAEASIAANPQKAMSDLPLLRAALKETLR 316814 (?)
316581 LYPVGGFLERILSSDLVLQNYHVPAG 316504 (0?)
316416 TLVLLYLYSMGRNPAVFPRPERYMPQRWLERKRSFQHLAFGFG 316396
316395 VRQCLGRRLAEVEMMLLLHH 316228 (0?)
315961 ILKTFQVETLRQEDVQMAYRFVLMPSSSPVLTFRPVS 315851
CYP11B3 rat
PIR JX0253 (498 amino acids)
Nomura,M., Morohashi, K.-i., Kirita, S., Nonaka, Y.,
Okamoto,M., Nawata,H. and Omura,T.
Three forms of rat CYP11B genes: 11 beta-hydroxylase gene,
aldosterone synthase gene, and a novel gene.
J. Biochem. 113, 144-152 (1993)
CYP11B3 rat
GenEMBL U14907 (1497bp)
Zhou,M., Gomez-Sanchez,E.P., Foecking,M. and Gomez-Sanchez,C.E.
Cloning of the cytochrome P-450 CYP11B3 complementary DNA in the
rat.
unpublished
CYP11B3 rat
GenEMBL S59144 D14098 to D14103
Mukai,K., Imai,M., Shimada,H. and Ishimura,Y.
Isolation and characterization of rat CYP11B genes involved
in late steps of mineralo- and glucocorticoid syntheses.
J. Biol. Chem. 268, 9130-9137 (1993)
Note: only one amino acid difference with Nomura's 11B3
CYP11B3 rat
GenEMBL U17082(1497bp)
Mellon,S.H., Bair,S.R. and Monis,H.
P450c11B3 mRNA, transcribed from a third P450c11 gene, is expressed
in a tissue-specific, developmentally, and hormonally regulated
fashion in the rodent adrenal and encodes a protein with both
11-hydroxylase and 18-hydroxylase activities.
J. Biol. Chem. 270, 1643-1649 (1995)
Note Genbank has a seq from rat named CYP11B4 D14108
This is part of the CYP11B8P pseudogene (see below)
CYP11B4 cattle
Kirita et al. 1990 J. Biochem (Tokyo) 108, 1030-1041.
Listed in 1996 nomenclature update
As having the same coding region sequence
But different flanking regions
CB11beta-20
CYP11B5P cattle
GenEMBL JU0316
Kirita et al. 1990 J. Biochem (Tokyo) 108, 1030-1041.
CB11beta-1 pseudogene
CYP11B6P cattle
GenEMBL JU0317
Kirita et al. 1990 J. Biochem (Tokyo) 108, 1030-1041.
CB11beta-3 pseudogene
CYP11B7P cattle
GenEMBL JU0318, D00458, D00459
Hashimoto et al. 1989 J. Biochem (Tokyo) 105, 676-679.
LambdaB11beta(15-1) and (15-2)
Kirita et al. 1990 J. Biochem (Tokyo) 108, 1030-1041.
CB11beta-21 pseudogene
CYP11B8P rat pseudogene
GenEMBL D14104 to D14108
Mukai,K., Imai,M., Shimada,H. and Ishimura,Y.
Isolation and characterization of rat CYP11B genes involved
in late steps of mineralo- and glucocorticoid syntheses.
J. Biol. Chem. 268, 9130-9137 (1993)
Note: authors call this sequence 11B4
12A Subfamily
CYP12A1 Musca domestica (housefly)
GenEMBL U86618
Rene Feyereisen
MIKYKQYSRAIVALRQRGAQQYSTNVTNASQPDVKATTTTTISP
EWQEAKPFEEMPSMNSWPIIKNMLPWGKYGKMEPTQFLMALRDDMGPIVRTAAFMGRP
PTVITHNPHDFEMVFRNEGIWPIRPGGDAQMYHRTVLREDFFQGVTGLVSVNGEKWGN
FRSTVNPVLMQPKNVRLYLNKMAQVNDEFMARIRQIRDPETLEVPASFQEEMNRWTLE
SVSVVALDKQLGLITTNRDNPDLKKLIGLLNDFFELGQKIEFGLPFWKYIKTPTFKLF
MKTLDGLLEIGNKYVNEAIDRLEAERQSGVPEKPENEKSVLEKLIKIDRKIATVMAID
MILAGVDTTSTTFTALLLCLAKNPEKQEKLREEIRQILPRKDSQFEPSSLNHIPYTRA
CIKEALRMYPLTLGNARILANDTVLSGYRVPKGTLVSMISTGLLQDDNHYTKAKEYLP
ERWMRPTKEETEDSATCPHALKASSPFIYLPFGFGPRSCVGRRIVEMELELGIARLVR
NFRIEFNYPTENAFKFKLINVPNIPLKFKFTDVEN
CYP12A2 Musca domestica (housefly)
GenEMBL U94698
Rene Feyereisen
CYP12A3 Musca domestica (housefly)
GenEMBL U94699
Rene Feyereisen
cyp12a4 Drosophila melanogaster
GenEMBL AC006091 85973-87865 also AC015190 AC008141
chromosome 3 clone BACR48G05 (D475)
MLKVRSALSLIQSQKATLSLATQKHTEYFKILLYIYINKLYYQRWQTNVATAEAREDSEW
LQAKPFEQIPRLNMWALSMKMSMPGGKYKNMELMEMFEAMRQDYGDIFFMPGIMGNPPFL
STHNPQDFEVVFRNEGVWPNRPGNYTLLYHREEYRKDFYQGVMGVIPTQGKPWGDFRTVV
NPVLMQPKNVRLYYKKMSQVNQEFVQRILELRDPDTLEAPDDFIDTINRWTLESVSVVAL
DKQLGLLKNSNKESEALKLFHYLDEFFIVSIDLEMKPSPWRYIKTPKLKRLMRALDGIQE
VTLAYVDEAIERLDKEAKEGVVRPENEQSVLEKLLKVDRKVATVMAMDMLMAGVDTTSST
FTALLLCLAKNPEKQARLREEVMKVLPNKNPEFTEASMKNVPYLRACIKESQRLHPLIVG
NARVLARDAVLSGYRVPAGTYVNIVPLNALTRDEYFPQASEFLPERWLRSPKDSESKCPA
NELKSTNPFVFLPFGFGPRMCVGKRIVEMELELGTARLIRNFNVEFNYPTENAFRSALIN
LPNIPLKFKFIDLPN
Cyp12a4 Drosophila pseudoobscura
See D. pseudoobscura page on this server for sequence
Obtained from UC Santa Cruz Browser
cyp12a5 Drosophila melanogaster
GenEMBL AC006091 83449-85296 also AC015190 AC008141
chromosome 3 clone BACR48G05 (D475)
76% identical to other AC006091 SEQ. 58% TO 12A1, 12A2
MLKGRIALNILQSQKPIVFSASQQ*RWQTNVPTAEIRNDPEWLQAKPFEE
IPKANILSLFAKSALPGGKYKNLEMMEMIDALRQDYGNIIFLPGMMGRDG
LVMTHNPKDFEVVFRNEGVWPFRPGSDILRYHRTVYRKDFFDGVQGIIPS
QGKSWGDFRSIVNPVLMQPKNVRLYFKKMSQVNQEFIKEIRDASTQEVPG
NFLETINRWTLESVSVVALDKQLGLLRESGKNSEATKLFKYLDEFFLHSA
DLEMKPSLWRYFKTPLLKKMLRTMDSVQEVTLKYVDEAIERLEKEAKEGV
VRPEHEQSVLEKLLKVDKKVATVMAMDMLMAGVDTTSSTFTALLLCLAKN
PEKQARLREEVMKVLPNKDSEFTEASMKNVPYLRACIKESQRVYPLVIGN
ARGLTRDSVISGYRVPAGTIVSMIPINSLYSEEYFPKPTEFLPERWLRNA
SDSAGKCPANDLKTKNPFVFLPFGFGPRMCVGKRIVEMELELGTARLIRN
FNVEFNHSTKNAFRSALINLPNIPLKFKFKFTDVPN*
Cyp12a5 Drosophila pseudoobscura
See D. pseudoobscura page on this server for sequence
Obtained from UC Santa Cruz Browser
CYP12A6 Drosophila wassermani
no accession number
Tina Yee and Phil Danielson
59% to 12A2 56% to 12A1
N-terminal does not match known P450 sequences may be in error
CYP12A7 Lucilia cuprina (Australian blowfly)
No accession number
Zhenzhong Chen
Submitted to nomenclature committee March 17, 2005
revised to full length June 27, 2005
Clone name Lc_CYP12A2
59% to 12A5
CYP12A8 Lucilia cuprina (Australian blowfly)
No accession number
Zhenzhong Chen
Submitted to nomenclature committee March 17, 2005
Clone name Lc_CYP12A1
60% to 12A5 67% to 12A7 (365 aa)
CYP12A9 Lucilia cuprina (Australian blowfly)
No accession number
Zhenzhong Chen
Submitted to nomenclature committee March 17, 2005
Revised seq Dec 12, 2007
Old Clone name Lc_CYP12A3
New clone name Luce0104J9 (408 aa)
74% identical to Musca Domestica CYP12A2
only 1 aa diff to earlier shorter version
CYP12A10 Lucilia cuprina (Australian blowfly)
No accession number
Zhenzhong Chen
Submitted to nomenclature committee March 17, 2005
Clone name Lc_CYP12A4
57% to 12A4 65% to 129A9 (126 aa)
CYP12A11 Lucilia cuprina (Australian blowfly)
No accession number
Zhenzhong Chen
Submitted to nomenclature committee Dec. 18, 2007
clone name Luce0102F03
62% identical to CYP12A2 and 57% identical to Dm CYP12a5,
63% to 12A7, 72% to 12A8 partial seq, 64% to 12A9 partial,
67% to 12A10 partial seq.
12B Subfamily
CYP12B1 Drosophila acanthoptera
no accession number
Phil Danielson
Ac40
submitted to nomenclature committee
Cyp12b2 Drosophila melanogaster
GenEMBL AC018326 7227-9141 also AC004345 AC004657
77% identical to 12b1
Cyp12b2 Drosophila pseudoobscura
See D. pseudoobscura page on this server for sequence
Obtained from UC Santa Cruz Browser
Cyp12c1 Drosophila melanogaster
GenEMBL AC009385 comp(57935-59646) also AC012807
Cyp12c1 Drosophila pseudoobscura
See D. pseudoobscura page on this server for sequence
Obtained from UC Santa Cruz Browser
Cyp12d1 Drosophila melanogaster
GenEMBL AC008187 comp(84371-86114)
Cyp12d1 Drosophila pseudoobscura
See D. pseudoobscura page on this server for sequence
Obtained from UC Santa Cruz Browser
CYP12D2 Lucilia cuprina (Australian blowfly)
No accession number
Zhenzhong Chen
Submitted to nomenclature committee Dec. 18, 2007
clone name Lce0054K20
53% identity to Dm CYP12d1
probable ortholog of Cyp12d1
Cyp12e1 Drosophila melanogaster
GenEMBL AC018294 2070-3932
Cyp12e1 Drosophila pseudoobscura
See D. pseudoobscura page on this server for sequence
Obtained from UC Santa Cruz Browser
CYP12F1 Anopheles gambiae (malaria vector)
No accession number
Submitted by Rene Feyereisen July 5, 2002
Low 40% range with other CYP12s 60% to 12F2
CYP12F1 Anopheles gambiae (malaria mosquito)
GenEMBL
Submitted by Christelle Abgrall, Hilary Ranson and Rene Feyereisen
See the paper: Evolution of supergene families associated with
insecticide resistance.
Ranson, H., Claudianos, C., Ortelli, F., Abgrall, C., Hemingway, J.
Sharakhova, M.V., Unger, M.F., Collins, F.H. and Feyereisen, R.
Science 298, 179-181 (2002)
Anopheles map name CYPl3r4
CYP12F2 Anopheles gambiae (malaria vector)
No accession number
Submitted by Rene Feyereisen July 5, 2002
Low 40% range with other CYP12s 60% to 12F1
CYP12F2 Anopheles gambiae (malaria mosquito)
GenEMBL
Submitted by Christelle Abgrall, Hilary Ranson and Rene Feyereisen
See the paper: Evolution of supergene families associated with
insecticide resistance.
Ranson, H., Claudianos, C., Ortelli, F., Abgrall, C., Hemingway, J.
Sharakhova, M.V., Unger, M.F., Collins, F.H. and Feyereisen, R.
Science 298, 179-181 (2002)
Anopheles map name CYPl3r3
CYP12F3 Anopheles gambiae (malaria vector)
No accession number
Submitted by Rene Feyereisen July 5, 2002
Low 40% range with other CYP12s 60% to 12F2
CYP12F3 Anopheles gambiae (malaria mosquito)
GenEMBL
Submitted by Christelle Abgrall, Hilary Ranson and Rene Feyereisen
See the paper: Evolution of supergene families associated with
insecticide resistance.
Ranson, H., Claudianos, C., Ortelli, F., Abgrall, C., Hemingway, J.
Sharakhova, M.V., Unger, M.F., Collins, F.H. and Feyereisen, R.
Science 298, 179-181 (2002)
Anopheles map name CYPl3r2
CYP12F4 Anopheles gambiae (malaria vector)
No accession number
Submitted by Rene Feyereisen July 5, 2002
Low 40% range with other CYP12s 55% to 12F2
CYP12F4 Anopheles gambiae (malaria mosquito)
GenEMBL
Submitted by Christelle Abgrall, Hilary Ranson and Rene Feyereisen
See the paper: Evolution of supergene families associated with
insecticide resistance.
Ranson, H., Claudianos, C., Ortelli, F., Abgrall, C., Hemingway, J.
Sharakhova, M.V., Unger, M.F., Collins, F.H. and Feyereisen, R.
Science 298, 179-181 (2002)
Anopheles map name CYPl3r1
CYP12F5 Aedes aegypti (yellow fever mosquito)
CYP12F6 Aedes aegypti (yellow fever mosquito)
CYP12F7 Aedes aegypti (yellow fever mosquito)
CYP12F8 Aedes aegypti (yellow fever mosquito)
CYP12F9 Culex_quinquefasciatus (southern house mosquito)
No accession number
Osamu Komagata
submitted to nomenclature committee 7/25/07
sequence 15
68% to 12F7 Aedes, 60% to 12F2
CYP12F9 Culex pipiens
CYP12F10 Culex pipiens
CYP12F11 Culex pipiens
CYP12F12 Culex pipiens
CYP12F13 Culex pipiens
CYP12F14 Culex pipiens
CYP12G1 Lucilia cuprina (Australian blowfly)
No accession number
Zhenzhong Chen
Submitted to nomenclature committee March 17, 2005
Clone name Lc_CYP12C
51% to 12C1 (310 aa)
CYP12H1 Tribolium castaneum (red flour beetle)
GenEMBL XP_966937, DT790363.1 EST
37% to 12c1, 38% to 12A4 mito clan, 42% to 12F2
MSTGSENVISQKKLDHYKNLNALENSDVMPEGWDKAKPFESLPGPKPIPFFGN
IWRFLVPKIGDFYGIDFLDLHKIFYEQYGDISILKGMINDPIVVIFNPKDYETLFRNEGIWPIRKGLQSF
NEYRKSRKDIFVNAGLLLEEGENWFKIRSVVNPILMQPRNISQYTDKMNSVADELVNNITNLLEESENGE
MPENFHNELYKWSLESVGLVTLDTHLGCLKNDLDEDSEPQKLIRSTLEMFKLMHKLDVLPSFHNYVSTPS
WRKFVQVMDFIVETNMKYVNQVLDKLEKGETTSTEIPSVLEKLLKVDRNIAVTMSIDMMIAGIDTTGRIL
GAALYFLGKNHEAQEKLRSEAISLLQTKDQAVTSEVLKKAPYLKAVIKETTRLAPIGIGNLRTTVKNLVL
GGYQIPKGTDVVTSNLVLCTNDEYFSRAKEFIPERWLSTTSGELSKKNTNPFIFAPFGYGPRSCVGKRLA
SLELEVALLKIIRNFELDWPHEDMVFKTKMLYGMTEPLKIHVRSV
CYP12J1 Leptinotarsa decemlineata (Colorado potato beetle)
No accession number
Jianhua Zhang
Submitted to nomenclature committee April 2, 2006
Clone name P12F5113
59 amino acids from heme to end
42% to 12A4, 57% to CYP12H1 XP_966937
5 aa diffs to CYP12J2
58% to XM_961844.1 PREDICTED: Tribolium castaneum
37% to 12c1, 38% to 12A4, 42% to 12f2
CYP12J2 Leptinotarsa decemlineata (Colorado potato beetle)
No accession number
Jianhua Zhang
Submitted to nomenclature committee April 2, 2006
Clone name P12F231134
59 amino acids from heme to end
43% to 12A4, 57% to CYP12H1 XP_966937 91% to 12J1
CYP12J3 Ips pini (pine engraver, bark beetle)
No accession number
Pamela Sandstrom
Submitted to nomenclature committee on April 26, 2006
Full length sequence
60% to 12J1 Colorado potato beetle (C-term piece)
44% to 12H1 Tribolium castaneum
clone name EST 28H12
CYP12K1 Nasonia vitripennis (jewel wasp)
See wasp page
CYP12L1 Lucilia cuprina (Australian blowfly)
No accession number
Zhenzhong Chen
Submitted to nomenclature committee Dec. 18, 2007
clone name Luce0117K18
46% identical to M. domestica CYP12A2
13B Subfamily
CYP13B1 Caenorhabditis elegans (nematode worm)
GenEMBL Z54269 (19692bp) and Z92827 (C29F7)
F02C12.5 also C29F7 has the end of this sequence
Formerly CYP16A1 but renamed after full length sequence sorted with
CYP13
CYP13B2 Caenorhabditis elegans (nematode worm)
GenEMBL Z81565
K06G5
14A Subfamily
CYP14A1 Caenorhabditis elegans (nematode worm)
GenEMBL Z50742 (20856bp)
Wilson,R., Ainscough,R., Anderson,K., Baynes,C., Berks,M.,
Bonfield,J., Burton,J., Connell,M., Copsey,T., Cooper,J.,
Coulson,A., Craxton,M., Dear,S., Du,Z., Durbin,R., Favello,A.,
Fulton,L., Gardner,A., Green,P., Hawkins,T., Hillier,L., Jier,M.,
Johnston,L., Jones,M., Kershaw,J., Kirsten,J., Laister,N.,
Latreille,P., Lightning,J., Lloyd,C., McMurray,A., Mortimore,B.,
O'Callaghan,M., Parsons,J., Percy,C., Rifken,L., Roopra,A.,
Saunders,D., Shownkeen,R., Smaldon,N., Smith,A., Sonnhammer,E.,
Staden,R., Sulston,J., Thierry-Mieg,J., Thomas,K., Vaudin,M.,
Vaughan,K., Waterston,R., Watson,A., Weinstock,L.,
Wilkinson-Sproat,J. and Wohldman,P.
2.2 Mb of contiguous nucleotide sequence from chromosome III of C.
elegans.
Nature 368 (6466), 32-38 (1994)
K09A11.2
CYP14A2 Caenorhabditis elegans (nematode worm)
GenEMBL Z50742 (20856bp)
see CYP14A1 for reference.
K09A11.3
CYP14A3 Caenorhabditis elegans (nematode worm)
GenEMBL Z50742 (20856bp)
see CYP14A1 for reference.
K09A11.4
CYP14A4 Caenorhabditis elegans (nematode worm)
GenEMBL Z50742 (20856bp) Z70212(R04D3.1 continuation of Z50742)
see CYP14A1 for reference.
after K09A11.5
partial sequence at end of cosmid continues on cosmid R04D3
CYP14A5 Caenorhabditis elegans (nematode worm)
GenEMBL U64847
F08F3.7
CYP15A1 Diploptera punctata (cockroach)
GenEMBL AY509244
Helvig,C., Koener,J.F., Unnithan,G.C. and Feyereisen,R.
CYP15A1, the cytochrome P450 that catalyzes epoxidation of methyl
farnesoate to juvenile hormone III in cockroach corpora allata
Unpublished
CYP15A1 Tribolium castaneum (red flour beetle)
GenEMBL XP_970303
44% to CYP15B1 Anopheles, 48% to CYP15B1 Aedes
note: CYP15A1, B1 and C1 are all probably orthologs.
Each species seems to have only a single CYP15.
MLFFVTLVISLVLLFLILDTIKPRRYPPGPKWLPIVGNFLEFRRRLSEIGYHHLVWKEFSEEYGDVVGLK
MGRNLVVAVFGAEAVKEVLTREEFDGRPDGFFFRLRTFGKRLGIVFSDGQFWQKQRKFSMQHLRNFGFGR
KEMEEKIEEETKDLIAVFKKQCSEPIWMHTAFDVSVLNVLWAMMAGERFNINDERLRKLLKIVHDAFRLT
DMSGGMLNQLPFLRFIAPETCGYNQLVDVLVRMWEFLQETISEHRKTLCSSHARDLIDAFLQKMDIQSDS
SFTDDQLMSLCLDLFMAGSETTSNTLGFSVVYMLQFPEVQKKVQDEMDEVVGRNRWPTLQDRIKLKYTEA
VLMEIQRRANIPPLGIAHRATRDVSLFDYRIPEGTIVLTSLYSVHMDHKFWKDPLAFRPERFLNKEGNLE
VDEKYFAPFGYGKRRCLGESLAKANYFLFFTALLHNFYLEKDCDGPEPQLEGYDGVTISPKPFRAKLIPRTD
CYP15A1 Pediculus humanus humanus (body louse)
No accession number
Reed Johnson and Barry Pittendrigh
Submitted to nomenclature committee March 14, 2007
Ph15, 49% to 15A1 Tribolium
Pediculus genome site
CYP15A1 Nasonia vitripennis (jewel wasp)
See wasp page
CYP15A1 Reticulitermes flavipes (termite)
No accession number
Michael Scharf
Submitted to nomenclature committee Feb. 19, 2009
Clone name RfCyp15A1-2
69% to Diploptera punctata CYP15A1
58% to CYP15A1 Tribolium, 48% to 15B1 Anopheles
CYP15B1 Anopheles gambiae (malaria mosquito)
GenEMBL
Submitted by Christelle Abgrall, Hilary Ranson and Rene Feyereisen
See the paper: Evolution of supergene families associated with
insecticide resistance.
Ranson, H., Claudianos, C., Ortelli, F., Abgrall, C., Hemingway, J.
Sharakhova, M.V., Unger, M.F., Collins, F.H. and Feyereisen, R.
Science 298, 179-181 (2002)
Anopheles map name CYPj2l3
47% identical to 15A1, though this is probably the ortholog of CYP15A1
CYP15B1 Aedes aegypti (yellow fever mosquito)
CYP15B1 Culex pipiens quinquefasciatus (southern house mosquito)
XM_001864994
locus_tag="CpipJ_CPIJ014944
72% to CYP15B1 Aedes aegypti
MRKPAFFPPGPAWFPLIGCGYRVFKLVNVHKFYHLMWDALARRY
GPVVGLKLGRDRVVIVSGLAAIREFYSKEAFNGRPNGFFFRIRSFNKRLGLVFNDGAD
WDVQRRFSVKTLKSLGMGRTGMVHSLEKETEELIHHLRKLSRGGRTIQMHSAFDIAVL
NVIWTLLAGNRFKLDDKRLAWICDTIHNSFRVIDMSGGVLNQCPPIRHICPNKSGFKP
LVNLLNPLLNFINDTISNIKANPECPERHNCLISSYLEELQIDNPHPSFSNEQLLSLC
LDLFQAGSETTSNTLGFGVAHVMHHPNVIAKIHQELDNVVGRYRLPTLDDRPSLPYLE
ATICEIQRIANVAPLAIAHRTTSPVQLGQYVIPRDTIAMVSLYSIHMDRQYWGDPEVF
RPERFLNESEDKLIQHEYFLPFGAGKRRCLGEALAKSNLFLFFAAFMHAFIVEPANPG
QLPPLEGFDGITLSPQPYQVRLKERLI
CYP15B1 Culex pipiens
CPIJ014944
CYP15C1 Bombyx mori (silkworm)
BAAB01071346.1 BAAB01157546.1 BAAB01036905.1 BAAB01022841.1
BAAB01157214.1BAAB01068330.1
40% to CYP15
note: CYP15A1, B1 and C1 are all probably orthologs. Each species
seems to only have a single CYP15. (not found in Drosophila)
See silkworm page for sequence
CYP15D1X Daphnia pulex (water flea)
Confidential
renamed CYP370A1
CYP15D2X Daphnia pulex (water flea)
Confidential
renamed CYP370A2
CYP15D3PX Daphnia pulex (water flea)
Confidential
renamed CYP370A3P
CYP15D4X Daphnia pulex (water flea)
Confidential
renamed CYP370A4
CYP15D5X Daphnia pulex (water flea)
Confidential
renamed CYP370A5
CYP15D6X Daphnia pulex (water flea)
Confidential
renamed CYP370A6
CYP15D7X Daphnia pulex (water flea)
Confidential
renamed CYP370A7
CYP15D8X Daphnia pulex (water flea)
Confidential
renamed CYP370A8
CYP15D9X Daphnia pulex (water flea)
Confidential
renamed CYP370A9
CYP15D10X Daphnia pulex (water flea)
Confidential
renamed CYP370A10
CYP15D11X Daphnia pulex (water flea)
Confidential
renamed CYP370A11
CYP15D12X Daphnia pulex (water flea)
Confidential
renamed CYP370A12
CYP15D13X Daphnia pulex (water flea)
Confidential
renamed CYP370A13
CYP15E1X Daphnia pulex (water flea)
Confidential
renamed CYP370B1
CYP15E2X Daphnia pulex (water flea)
Confidential
renamed CYP370B2
CYP15F1 Reticulitermes flavipes (termite)
No accession number
Michael Scharf
Submitted to nomenclature committee Feb. 19, 2009
Clone name RfCyp15A1-1
43% to CYP15A1 Tribolium, 38% TO CYP15B1 Anopheles
16A Subfamily
CYP16A1X Caenorhabditis elegans (nematode worm)
GenEMBL Z54269
number retired
This sequence is now CYP13B1
17A Subfamily
CYP17 human
GenEMBL M14564
Chung,B.C., Picado-Leonard,J., Haniu,M., Bienkowski,M., Hall,P.F.,
Shively,J.E. and Miller,W.L. (1987)
Cytochrome P450c17 (steroid 17 alpha-hydroxylase/17,20 lyase):
cloning of human adrenal and testis cDNAs indicates the same gene
is expressed in both tissues
Proc. Natl. Acad. Sci. U.S.A. 84, 407-411.
First human CYP17A1 cDNA
CYP17 human
GenEMBL M19489
Picado-Leonard J, Miller WL. (1987) Cloning and sequence of the human
gene for P450c17 (steroid 17 alpha-hydroxylase/17,20 lyase):
similarity with the gene for P450c21.
DNA. 6. 439-448.
First human CYP17A1 gene
CYP17 human
GenEMBL NM_000102
MWELVALLLLTLAYLFWPKRRCPGAKYPKSLLSLPLVGSLPFLP
RHGHMHNNFFKLQKKYGPIYSVRMGTKTTVIVGHHQLAKEVLIKKGKDFSGRPQMATL
DIASNNRKGIAFADSGAHWQLHRRLAMATFALFKDGDQKLEKIICQEISTLCDMLATH
NGQSIDISFPVFVAVTNVISLICFNTSYKNGDPELNVIQNYNEGIIDNLSKDSLVDLV
PWLKIFPNKTLEKLKSHVKIRNDLLNKILENYKEKFRSDSITNMLDTLMQAKMNSDNG
NAGPDQDSELLSDNHILTTIGDIFGAGVETTTSVVKWTLAFLLHNPQVKKKLYEEIDQ
NVGFSRTPTISDRNRLLLLEATIREVLRLRPVAPMLIPHKANVDSSIGEFAVDKGTEV
IINLWALHHNEKEWHQPDQFMPERFLNPAGTQLISPSVSYLPFGAGPRSCIGEILARQ
ELFLIMAWLLQRFDLEVPDDGQLPSLEGIPKVVFLIDSFKVKIKVRQAWREAQAEGST
CYP17 human EST
GenEMBL Z19875 (235bp)
UK-HGMP (United Kingdom human genome mapping project)
covers amino acids 270-348 when translating the complementary
strand. The fragment goes through at least 6 frame shifts.
sequence ID AAAAWEO
CYP17 human EST
GenEMBL Z20209 (248bp)
UK-HGMP (United Kingdom human genome mapping project)
covers amino acids 265-349 when translating the complementary
strand. The fragment goes through at least 8 frame shifts.
sequence ID AAABPSZ
CYP17 human
GenEMBL S85459 (556bp)
Biason,A., Mantero,F., Scaroni,C., Simpson,E.R. and Waterman,M.R.
Deletion within the CYP17 gene together with insertion of foreign
DNA is the cause of combined complete 17
alpha-hydroxylase/17,20-lyase deficiency in an Italian patient
Mol. Endocrinol. 5, 2037-2045 (1991)
CYP17A1 Macaca fasicularis (cynomolgus monkey)
No accession number
Yasuhiro Uno
Submitted to nomenclature committee 1/11/2005
Clone name mfCYP17A1
94% to human, missing first 9 aa
CYP17 rat
PIR A31359 (507 amino acids)
Namiki, M., Kitamura, M., Buczko, E. and Dufau, M.L.
Rat testis P-450-17-alpha cDNA: the deduced amino acid
sequence, expression and secondary structural configuration.
Biochem. Biophys. Res. Commun. 157, 705-712 (1988)
CYP17 rat
PIR D41425 (16 amino acids)
Imaoka, S., Kamataki, T. and Funae, Y.
Purification and characterization of six cytochromes P-450
from hepatic microsomes of immature female rats.
J. Biochem. 102, 843-851 (1987)
CYP17 rat
GenEMBL S50146 Z11902 (3345bp)
PIR S20655 (97 amino acids)
Nason,T.F., Han,X.G. and Hall,P.F.
Cyclic AMP regulates expression of the rat gene for steroid
17 alpha-hydroxylase/C17-20 lyase P-450 (CYP17) in rat Leydig
cells.
Biochim. Biophys Acta 1171, 73-80 (1992)
Note: have not been able to download S50146 from GCG or NCBI.
CYP17 rat
GenEMBL X69816 (7556bp)
Givens,C.R., Zhang,P., Bair,S.R. and Mellon,S.H.
Transcriptional regulation of rat cytochrome P450c17 expression in
mouse Leydig MA-10 and adrenal Y-1 cells: identification of a
single protein that mediates both basal and cAMP-induced activities.
DNA Cell Biol. 13, 1087-1098 (1994)
CYP17 rat
PIR S24316 (97 amino acids)
Zhang, P., Nason, T.F., Han, X.G. and Hall, P.F.
Gene for 17-alpha-hydroxylase/C(17-20) lyase P-450: complete
nucleotide sequence of the porcine gene and 5' upstream
sequence of the rat gene.
Biochim. Biophys. Acta 1131, 345-348 (1992)
Cyp17 mouse
genEMBL M64863 AC079419.1 Mm.1262
MWELVGLLLLILAYFFWPKSKTPNAKFPRSLPFLPLVGSLPFLP
RRGHMHANFFKLQEKYGPIYSLRLGTTTAVIVGHYQLAREVLVKKGKEFSGRPQMVTL
GLLSDQGKGVAFADSSSSWQLHRKLVFSTFSLFRDDQKLEKMICQEANSLCDLILTYD
GESRDLSTLIFKSVINIICTICFNISFENKDPILTTIQTFTEGIVDVLGHSDLVDIFP
WLKIFPNKNLEMIKEHTKIREKTLVEMFEKCKEKFNSESLSSLTDILIQAKMNAENNN
TGEGQDPSVFSDKHILVTVGDIFGAGIETTSSVLNWILAFLVHNPEVKRKIQKEIDQY
VGFSRTPSFNDRTHLLMLEATIREVLRIRPVAPLLIPHKANIDSSIGEFAIPKDTHVI
INLWALHHDKNEWDQPDRFMPERFLDPTGSHLITPTPSYLPFGAGPRSCIGEALARQE
LFIFMALLLQRFDFDVSDDKQLPCLVGDPKVVFLIDPFKVKITVRQAWKDAQVEVST
CYP17 hamster
no accession number
Cloutier,M., Fleury,A., Courtemanche,J., Ducharme,L.
Mason,J.I. and Lehoux,J.G.
Cloning and expression of hamster adrenal cytochrome P450c17 cDNA.
Ann. N.Y. Acad. Sci. 774, 294-296 (1995)
CYP17 Cavia (guinea pig)
GenEMBL S75277(1732bp)
Tremblay,Y., Fleury,A., Beaudoin,C., Vallee,M. and Belanger,A.
Molecular cloning and expression of guinea pig cytochrome P450c17
cDNA (steroid 17 alpha-hydroxylase/17,20 lyase): tissue
distribution, regulation, and substrate specificity of the
expressed enzyme.
DNA Cell Biol. 13 (12), 1199-1212 (1994)
CYP17 Cavia porcellus (guinea pig)
PIR S52756 (508 amino acids)
Huang,Y., Voigt,J.M. and Colby,H.D.
unpublished
CYP17 pig
GenEMBL Z11854 to Z11856 GenEMBL S40341 (1858bp)
PIR S24233 (501 amino acids) PIR S30074 (501 amino acids)
Zhang,P. Nason,T.F., Han,X.G. and Hall,P.F.
Gene for 17 alpha-hydroxylasae/C(17-20) lyase P-450: complete
nucleotide sequence of the porcine gene and 5' upstream sequence of
the rat gene.
Biochim. Biophys. Acta 1131, 345-348 (1992)
CYP17 Sus scrofa (pig)
GenEMBL U41519 to U41525
Conley,A.J., Graham-Lorence,S.E., Kagimoto,M., Lorence,M.C.,
Murry,B.A., Oka,K., Sanders,D. and Mason,J.I.
Nucleotide sequence of a cDNA encoding porcine testis 17
alpha-hydroxylase cytochrome P-450.
Biochim. Biophys. Acta 1130, 75-77 (1992)
MWVLLVFFLLTLTYLFWPKTKGSGAKYPRSLPVLPVVGSLPFLP
RRGHQHMNFFKLQDKYGPIFSFRLGSKTTVVIGDHQLAKEVLLKKGKEFSGRPRVMTL
DILSDNQKGIAFADHGTSWQLHRKLALSTFSLFKGGNLKLENIINQEIKVLCDFLATR
NGESIDLAQPLSLAMTNIVSFICFNFSFKKGDPALQAIVNFNDGILDAVGKEILYDMF
PGIRILPSQTLENMKQCVRMRNELLREILENRKENYSRNSITNLLDIMIQAKTNAESN
TGGPDHNLKLLSDRHMLATVADIFGAGVETSASVVKWIVAFLLHYPLLRKKIQDAIDQ
NIGFNRAPSISDRNQLVLLEATIREVLRFRPVSPTLIPHRAIIDSSIGEFTIDKDTDV
VVNLWALHHNEKEWHRPDLFMPERFLDPTGTQLISPSLSYLPFGAGPRSCVGEMLARQ
ELFLFTAGLLQRFDLELPDDGQLPCLVGNPSLVLQIDPFKVKIKERQAWKEAHTEGSTS
CYP17 bovine
GenEMBL M64646 (1725bp)
Zuber,M.X., John,M.E., Okamura,T., Simpson,E.R. and Waterman,M.R.
Bovine adrenocortical cytochrome P-450-17-alpha: Regulation of gene
expression by ACTH and elucidation of primary sequence
J. Biol. Chem. 261, 2475-2482 (1986)
CYP17A1 Bos taurus (cow)
See cattle page for details
MWLLLAVFLLTLAYLFWPKTKHS
116 GAKYPRSLPSLPLVGSLPFLPRRGQQHKNFFKLQEKYGPIYSFRLGSKTTVMIGHHQLAR 295
296 EVLLKKGKEFSGRPKVATLDILSDNQKGIAFADHGAHWQLHRKLALNAFALFKDG 460
461 NLKLEKIINQEANVLCDFLATQHGEAIDLSEPLSLAVTNIISFICFNFSFKNEDP 625
626 ALKAIQNVNDGILEVLSKEVLLDIFPVLKIFPSKAMEKMKGCVQTRNELLNEILEKCQEN 805
806 FSSDSITNLLHILIQAKVNADNNNAGPDQDSKLLSNRHMLATIGDIFGAGVETTTSVIK 982
983 WIVAYLLHHPSLKKRIQDDIDQIIGFNRTPTISDRNRLVLLEATIREVLRIRPVAPTLIP 1162
1163 HKAVIDSSIGDLTIDKGTDVVVNLWALHHSEKEWQHPDLFMPERFLDPTGTQLISPSL 1336
1337 SYLPFGAGPRSCVGEMLARQELFLFMSRLLQRFNLEIP 1450
DDGKLPSLEGHASLVLQIKPFKVKIEVRQAWKEAQAEGSTP*
CYP17 Ovis aries (sheep)
GenEMBL L40335 (1728bp)
Murry,B.A., Swart,P. and Mason,J.I.
Cloning and expression of ovine cytochrome P-450 17-alpha
hydroxylase/c17-20 lyase.
Unpublished (1995)
CYP17 Equus caballus (horse)
GenEMBL D30688(1906bp) D13818
Hasegawa,T., Mukoyama,H., Yoshida,S. and Takahashi,M.
Molecular cloning and nucleotide sequence of equine testicular
cytochrome P-450 steroid 17alpha-hydroxylase/C17,20-lyase messenger
ribonucleic acid.
Biol. Reprod. Mono. 1, 615-622 (1995)
CYP17 Equus caballus (horse)
GenEMBL D88184(6217bp)
Hasegawa,T.
Exon/Intron structure of Equine P450c17.
unpublished (1996)
CYP17A1 Xenopus tropicalis
See Xenopus pages for seq
CYP17A1 Danio rerio (zebrafish)
See xebrafish pages for seq
CYP17 Oncorhynchus mykiss (rainbow trout)
GenEMBL S50356
Sakai,N., Tanaka,M., Adachi,S., Miller,W.L. and Nagahama,Y.
Rainbow trout cytochrome P-450c17 (17 alpha-hydroxylase/17-20
lyase) cDNA cloning, enzymatic properties and temporal pattern of
ovarian P-450c17 mRNA expression during oogenesis.
FEBS Lett. 301, 60-64 (1992)
Identical to X65800
CYP17 Oncorhynchus mykiss (rainbow trout)
GenEMBL X65800 (2287bp) Swiss P30437 (514 amino acids)
Sakai,N., Tanaka,M., Adachi,S., Miller,W.L. and Nagahama,Y.
Rainbow trout cytochrome P-450c17 (17
alpha-hydroxylase/17,20-lyase). cDNA cloning, enzymatic properties
and temporal pattern of ovarian P-450c17 mRNA expression during
oogenesis
FEBS Lett. 301, 60-64 (1992)
Identical to S50356
CYP17 Oryzias latipes (medaka)
GenEMBL D87121(2421bp)
Kobayashi,D., Matsuyama,M., Tanaka,M., Fukada,S. and Nagahama,Y.
Structural analysis of medaka P-450c17 and expression in the
ovarian follicle.
unpublished (1996)
CYP17 Oryzias latipes (medaka)
GenEMBL D87122(2302bp)
Kobayashi,D., Tanaka,M., Fukada,S. and Nagahama,Y.
Presence of a Novel Cytochrome P-450c17 Transcripts in Medaka
Gonads
unpublished (1996)
note: this sequence is missing exon 6
otherwise identical to D87121
CYP17 Squalus acanthias (dogfish)
GenEMBL S77384 (1964bp)
Trant,J.
Isolation and characterization of the cDNA encoding the spiny
dogfish shark
(Squalus acanthias) form of cytochrome P450c17
J. Exp. Zool. 272, 25-33 (1995)
CYP17 Ictalurus punctatus (channel catfish)
GenEMBL AF063837
Trant,J.M., Berard,C., Byrne,B.J. and Wunder,J.
Isolation and heterologous expression of the cDNA encoding the
cytochrome P450 17-hydroxylase from the channel catfish (Ictalurus
punctatus)
Unpublished (1998)
CYP17A1 Fugu rubripes (pufferfish)
No accession number
Scaffold_4175
80% to Oryzias latipes 17, 79% to trout 17 73% to catfish
61% to dogfish 48% to other CYP17 from Fugu
MDWVLFVYAFSAVNLALLALHLKFRTPASGPRGPPRLPALPLIGSLLSLRSPHPPHVLFKE
LQGKYGQTYSLMMGSHRVIIVNHHAHAKEVLLKKGKIFAGRPRS 11667 (0)
11572 VTTDVLSRDGKDIAFGDYSATWRFHRKIVHGALCMFGEGSASIEKI 11435 (1)
10871 ICAEAASLCSILSEAWTAGLALDLSPELTRAVTNVICSLCFSSSYRRGDAEFEAMLHYSQ 10692
10691 GIVDTVAKDSLVDIFPCLQ 10635 (0)
IFPNADLRLLKRCVSVRDKLLQKEYDKHK (0)
AAYSDHVQRDLLDALLRAKCSAENNNTTGINAESVGLTDDHLLMT
VGDIFGAGVETTTTVMKWAITYLIHHPQ 9765 (0)
9637 IQSRIQEELDSRVGMDRSPQLSDRGSLPYLEATIREVLRIRPVAPLFIPHVALSDT 9473 (2)
9369 SIGDFAVKKGTRVVINLWSLHHDEKEWENPERFDP 9265 (1)
GRFLNSEGTGLVIPSSSYLPFGAGVRVCLGEALAK 8558
8557 MELFLFLSWILQRFTLTVPSGHSLPSLEGKFGVVLQPTKYKVNATPRPGWEGKCKACWN* 8378
CYP17A2 Danio rerio (zebrafish)
See xebrafish pages for seq
CYP17A2 Fugu rubripes (pufferfish)
No accession number
Scaffold_8086
50% to Oryzias latipes 17, 48% to trout 17 50% to catfish
49% to dogfish 48% to other CYP17 from Fugu
1198 MVTVGSFLIFRRPVRGSEPGSEAGPPRVKVPCISWVPVLGSLPWLRGGRPLHLIFTQLSYR 1380 (2)
1600 YGPLFALYLGPHLTVVVNNHQHAREVLLLRGKDFAGRPRM 1719 (0)
1797 VTTDLLTRGGKDIAFSDYCPLWKSHRRLVQNSFTLFGEGTSRLQDM 1934 (1)
2061 VLAAVDSLCEELLSMEGRGFDPAPAVTRAVTNVVCMLVFSATYRHGDSELQEVLRYNDGI 2240
2241 VQTIAGGGLVDIYPWMK 2291 (0)
2372 VFPNKTLSKLKACIAVRDRLLTHKLEEHK 2458 (0)
2622 ATLTDNQPRDLLDALLMGQVGRGRRKGSGRVEEDIITEDHVLMTAAEAFGAGVETTSTTLLWILAYLLHHPQ 2837 (0)
2925 VQERVQKELDDHVGSERPVRVSDRARLTYLDCVINEGMRIRPVSPVLIPHTAMTDSR 3074 (2)
3870 IGGHHISRGTRVLVNMWSIHHDSAHWDKPDLFNP 3971 (1)
4519 DRFRDHQGQRVTPSCFLPFGAGPRVCVGESLARLELFLFLSSLLQRMSFRLPNGA 4692
4693 SPPDLQGRMGVVLQPVPYKVVVTPRVG* 4776
CYP17 fragment a Fugu rubripes (pufferfish)
No accession number
Fc:c028I22x2 LPC.10549.x2 Length = 1007 61% to 17A1 Fugu
GAPRATTDNHHAHAKEARPKKGKKAAGRPRK exon 1
ATTDGASRDGTDTAEGDHSATRRDQR exon 2
CYP17 fragment b Fugu rubripes (pufferfish)
No accession number
Fc:c028I22x1 LPC.10549.x1 Length = 894
78% to Fc:c028I22x2 65% to 17A1 Fugu
KEQQAEHGQTTSRMMGSHRGNTDNQHAHAKEARQKKGKKGAGRPRK exon 1
ATTDGRSRDGTDIAEGDHSATWRHHRKIVQRARRRNGEGSAPSEKI exon 2 C-helix
18A Subfamily
Cyp18a1 Drosophila melanogaster
GenEMBL S66112 (63bp)
Hurban,P. and Thummel,C.
Isolation and characterization of fifteen ecdysone-inducible
drosophila genes reveal unexpected complexities in ecdysone regulation.
Molec. Cell Biol. 13, 7101-7111 (1993)
Note: very short ecdysone inducible fragment in the heme binding
region about 2/3 of amino acids are identical to 2D sequences.
called Eig 17-1
dimethylnitrosamine demethylase
Cyp18a1 Drosophila melanogaster
GenEMBL U44753(2539bp)
Bassett,M.H, Waterman,M.R., McCarthy,J.L. and Sliter,T.J.
Cloning and characterization of CYP18 in Drosophila melanogaster:
identification of an insect member of a new cytochrome P450 family.
unpublished (1996)
complete sequence from the Eig 17-1 fragment
Cyp18a1 Drosophila melanogaster
GenEMBL AC012164 114600-117669 also AC015216
Cyp18a1 Drosophila pseudoobscura
See D. pseudoobscura page on this server for sequence
Obtained from UC Santa Cruz Browser
CYP18A1 Spodoptera littoralis (cotton leafworm)
No accession number
Lyndsay Davies
Submitted to nomenclature committee 2/22/02
61% identical to CYP18 from Drosophila
since there is only one CYP18 seq in Drosophila this new sequence
will be called CYP18 without a subfamily or number like CYP18A1.
If more CYP18s are found in a single species this may have to change.
CYP18A1 Apis mellifera (honeybee)
WGS section genbank
Submitted by May Berenbaum 2/11/04
AmGroup13.1a
60% to 18A1 Drosophila melanogaster.
CYP18A1 Bombyx mori (silkworm)
AU005208 EST BAAB01190855.1 BAAB01187772.1
60% to CYP18A1 Drosophila
See silkworm page for sequence
CYP18A1 Tribolium castaneum (red flour beetle)
GenEMBL XP_968393
58% to 18A1 Drosophila melanogaster
MFVYSGLVLWNFLAEELSTKVLAVFLMVLFLVRLVQMLVQMLKEASALPPGPWGLPILGSLPFLKGDLHL
HFRDLTHKYGSLISTRLGSQLIVVLSDYKMIRDAFRKEEFTGRPITEFTTLLDGYGVINTAGKLWKDQRR
FLHDGLRHFGMSYIGSRKTQMENRIMREVEEFLSVLTARKDTPIDLNPVLAVSLSNVICDILMSVRFSHN
DERFKRFMFLIDEGFKLFSSLEASFFIPILKYLPGQRQTREKIAKNRAEMAQFLQETIEEHRKSFDPSHL
RDLLDTYLYEIQKADEEGTGDHLFEGKDHDRQMQQIMGDLFSAGMETIKSSLQWAVLFMLHHPEVMKAVQ
EELDQVVGRRRLPKLEDLPYLPVTESTMLEVLRISSIVPMGTTHAPTRDLKLNGFHLPRHAQIVPLLHSV
HMDPSLWHEPERFNPSRFINAEGKVVKPEYFLPFGVGRRMCLGEILARMEIFSFFSSLLHSFDICVPTGE
TLPSLKGVAGVTISPNAFRVCLKPRPMEWDSMGTIRPAGSH
CYP18A1 Aedes aegypti (yellow fever mosquito)
CYP18A1 Culex pipiens
CPIJ001038
CYP18A1 Pediculus humanus humanus (body louse)
No accession number
Reed Johnson and Barry Pittendrigh
Submitted to nomenclature committee March 14, 2007
Ph18, 56% to 18A1 Apis
Pediculus genome site
CYP18A1 Nasonia vitripennis (jewel wasp)
See wasp page
CYP18A1 Daphnia pulex (water flea)
Confidential
CYP18B1 Bombyx mori (silkworm)
BAAB01081335.1 BAAB01007952.1
BAAB01142048.1 BAAB01138082.1 BAAB01045663.1
39% to CYP18A1 Bombyx
See silkworm page for sequence
CYP18B2 Manduca sexta
No accession number
Yannick Pauchet
Submitted to nomenclature committee April 7, 2009
confidential
19A Subfamily
CYP19 human
GenEMBL S52034 (142bp) S52789 (106bp) S52793 (149bp)
S52794 (125bp)
Harada,N.
A unique aromatase (P-450AROM) mRNA formed by alternative
use of tissue-specific exons 1 in human skin fibroblasts.
Biochem. Biophys. Res. Commun. 189, 1001-1007 (1992)
CYP19 human
GenEMBL D14473 (295bp) S59092 S59095 S59171
Toda,K and Shizuta,Y.
Molecular cloning of a cDNA showing alternative splicing of
the 5'-untranslated sequence of mRNA for human aromatase P-450.
Eur. J. Biochem. 213, 383-389 (1993)
CYP19 human
GenEMBL D13391 (2238bp)
Katsumi,T. and Shizuta,Y.
Identification and characterization of cis-acting regulatory
elements for
the expression of the human aromatase cytochrome P-450 gene.
J. Biol. Chem. 269, 8099-8107 (1994)
CYP19 human
GenEMBL D21240 (794bp) D21241 (3231bp)
Harada,N., Utsumi,T. and Takagi,Y.
Tissue-specific expression of the human aromatase cytochrome P-450
gene by alternative use of multiple exons 1 and promoters, and
switching of tissue-specific exons 1 in carcinogenesis.
Proc. Natl. Acad. Sci. U.S.A. 90 (23), 11312-11316 (1993)
CYP19 human
GenEMBL X55983 (669bp)
Toda,K., Miyahara,K., Kawamoto,T., Ikeda,H., Sagara,Y. and
Shizuta,Y.
Characterization of a cis-acting regulatory element involved in
human-
aromatase P-450 gene expression.
Eur, J. Biochem. 205, 303-309 (1992)
exon 1
CYP19 human
GenEMBL S71536 (792bp)
Toda,K., Simpson,E.R., Mendelson,C.R., Shizuta,Y. and Kilgore,M.W.
Expression of the gene encoding aromatase cytochrome P450 (CYP19)
in fetal tissues
Mol. Endocrinol. 8, 210-217 (1994)
CYP19 human
GenEMBL M32245 (840bp)
Harada,N., Yamada,K., Saito,K., Kibe,N., Dohmae,S. and Takagi,Y.
Structural characterization of the human estrogen synthetase
(aromatase) gene.
Biochem. Biophys. Res. Commun. 166, 365-372 (1990)
CYP19 human
GenEMBL D29757 (875bp) PIR PC2041 (45 amino acids)
Honda,S.-I., Harada,N. and Takagi,Y.
Novel exon 1 of the aromatase gene specific for aromatase
transcripts
in human brain.
Biochem. Biophys. Res. Commun. 198, 1153-1160 (1994)
CYP19 human
GenEMBL M22246 (2966bp)
Harada,N.
Cloning of a complete cDNA encoding human aromatase: immunochemical
identification and sequence analysis
Biochem. Biophys. Res. Commun. 156, 725-732 (1988)
CYP19 human
GenEMBL L21982 (1166bp)
Mahendroo,M.S., Mendelson,C.R. and Simpson,E.R.
Tissue-specific and hormonally-controlled alternative promoters
regulate aromatase cytochrome P450 gene expression in human adipose
tissue
J. Biol. Chem. 268, 19463-19470 (1993)
CYP19 human
GenEMBL S85356 (1384bp)
Means,G.D., Kilgore,M.W., Mahendroo,M.S., Mendelson,C.R. and
Simpson,E.R.
Tissue-specific promoters regulate aromatase cytochrome P450 gene
expression in human ovary and fetal tissues.
Mol. Endocrinol. 5, 2005-2013 (1991)
CYP19 human
GenEMBL S96437 (971bp)
Kilgore,M.W., Means,G.D., Mendelson,C.R. and Simpson,E.R.
Alternative promotion of aromatase P-450 expression in the human
placenta.
Mol. Cell. Endocrinol. 83, R9-R16 (1992)
CYP19 human
PIR A40542 (48 amino acids)
Mahendroo, M.S., Means, G.D., Mendelson, C.R. and Simpson, E.R.
Tissue-specific expression of human P-450-AROM. The promoter
responsible for expression in adipose tissue is different
from that utilized in placenta.
J. Biol. Chem. 266, 11276-11281 (1991)
CYP19 Macaca fuscata (Japanese macaque)
GenEMBL S79807(369bp)
Yamada-Mouri,N., Hirata,S., Hayashi,M. and Kato,J.
Analysis of the expression and the first exon of aromatase mRNA in
monkey brain.
J. Steroid Biochem. Mol. Biol. 55 (1), 17-23 (1995)
CYP19 rat
GenEMBL S59505 (639bp)
Fitzpatrick,S.L. and Richards,J.S.
cis-acting elements of the rat aromatase promoter required for
adenosine 3',5'-monophosphate induction in ovarian granulosa cells
and constitutive expression in R2C Leydig cells.
Molec. Endocrinol. 7, 341-354 (1993)
Note: promoter
CYP19 rat
GenEMBL Z11815 (590bp)
Hickey,G.J., Krasnow,J.S., Beattie,W.G. and Richards,J.S.
Aromatase cytochrome P450 in rat ovarian granulosa cells before and
after luteinization: Adenosine 3',5'-monophosphate-dependent and
independent regulation. Cloning and sequencing of rat aromatase
cDNA and 5' genomic DNA
Mol. Endocrinol. 4, 3-12 (1990)
CYP19 Oryctolagus cuniculus (rabbit)
GenEMBL Z68271(1783bp)
Delarue,B., Mittre,H., Feral,C., Benhaim,A. and Leymarie,P.
Rapid sequencing of rabbit aromatase cDNA using RACE PCR without
cloning.
C. R. Acad. Sci. III, Sci. Vie 319, 663-670 (1996)
CYP19 Oryctolagus cuniculus (rabbit)
GenEMBL Z70302(1455bp)
Delarue,B., Mittre,H. and Leymarie,P.
Expression des transcrits codant pour l'aromatase de lapin dans
differents tissus.
unpublished (1996)
Cyp19 mouse
Swiss P28649 (503 amino acids) GenEMBL D00659 (2420bp)
Terashima,M., Toda,K., Kawamoto,T., Kuribayashi,I., Ogawa,Y.,
Maeda,T. and Shizuta,Y.
Isolation of a full-length cDNA encoding mouse aromatase P450
Arch. Biochem. Biophys. 285, 231-237 (1991)
MFLEMLNPMQYNVTIMVPETVTVSAMPLLLIMGLLLLIWNCESS
SSIPGPGYCLGIGPLISHGRFLWMGIGSACNYYNKMYGEFMRVWISGEETLIISKSSS
MFHVMKHSHYISRFGSKRGLQCIGMHENGIIFNNNPSLWRTIRPFFMKALTGPGLVRM
VEVCVESIKQHLDRLGEVTDTSGYVDVLTLMRHIMLDTSNMLFLGIPLDESAIVKKIQ
GYFNAWQALLIKPNIFFKISWLYRKYERSVKDLKDEIAVLVEKKRHKVSTAEKLEDCM
DFATDLIFAERRGDLTKENVNQCILEMLIAAPDTMSVTLYFMLLLVAEYPEVEAAILK
EIHTVVGDRDIKIEDIQNLKVVENFINESMRYQPVVDLVMRRALEDDVIDGYPVKKGT
NIILNIGRMHRLEYFPKPNEFTLENFEKNVPYRYFQPFGFGPRGCAGKYIAMVMMKVV
LVTLLRRFQVKTLQKRCIENIPKKNDLSLHPNEDRHLVEIIFSPRNSDKYLQQ
CYP19 pig
no accession number
Corbin, C.J., Khalil, M.W. and Conley, A.J.
Functional ovarian and placental isoforms od porcine aromatase.
Mol. Cell. Endocrinol. 113, 29-37 (1995)
CYP19 Sus scrofa (pig)
GenEMBL L15471 (454bp)
Ko,Y., Choi,I., Green,M.L., Simmen,F.A. and Simmen,R.C.
Transient expression of the cytochrome P450 aromatase gene in
elongating porcine blastocysts is correlated with uterine
insulin-like growth factor levels during peri-implantation
development.
Mol. Reprod. Dev. 37 (1), 1-11 (1994)
Note: This is only a fragment of 80 amino acids, including
helix K and the EXXR conserved sequence.
CYP19 Sus scrofa (pig)
GenEMBL U52141(1133bp)
Choi,I., Collante,W., Simmen,R.C.M. and Simmen,F.A.
Molecular cloning of multiple forms of cytochrome p450 aromatase
and their developmental expression in porcine blastocysts,
endometrium, and placenta.
Unpublished (1997)
CYP19 Sus scrofa (pig)
GenEMBL U52142(1584bp)
Choi,I., Collante,W., Simmen,R.C.M. and Simmen,F.A.
Molecular cloning of multiple forms of cytochrome p450 aromatase
and their developmental expression in porcine blastocysts,
endometrium, and placenta.
Unpublished (1997)
CYP19 Sus scrofa (pig)
GenEMBL U37309 (417bp)
Choi,I., Simmen,R.C. and Simmen,F.A.
Molecular cloning of cytochrome P450 aromatase complementary
deoxyribonucleic acid from periimplantation porcine and equine
blastocysts identifies multiple novel 5'-untranslated exons
expressed in embryos, endometrium, and placenta.
Endocrinology 137 (4), 1457-1467 (1996)
CYP19 Sus scrofa (pig)
GenEMBL U37311(2470bp)
Choi,I., Simmen,R.C. and Simmen,F.A.
Molecular cloning of cytochrome P450 aromatase complementary
deoxyribonucleic acid from periimplantation porcine and equine
blastocysts identifies multiple novel 5'-untranslated exons
expressed in embryos, endometrium, and placenta.
Endocrinology 137 (4), 1457-1467 (1996)
CYP19 Sus scrofa (pig)
GenEMBL U57510
Choi,I., Collante,W., Simmen,R.C.M., Troyer,D. and Simmen,F.A.
Molecular cloning and structural characterization of porcine
cytochrome p450 aromatase chromosomal genes: evidence for the
existence of multiple, closely related genes that encode
developmental and tissue-specific isoforms of aromatase.
unpublished (1997)
CYP19 Sus scrofa (pig)
GenEMBL U57517(287bp) U57518(287bp) U57519(358bp)
Choi,I., Collante,W., Simmen,R.C.M., Troyer,D. and Simmen,F.A.
Molecular cloning and structural characterization of porcine
cytochrome p450 aromatase chromosomal genes: evidence for the
existence of multiple, closely related genes that encode
developmental and tissue-specific isoforms of aromatase.
unpublished (1997)
CYP19 Sus scrofa (pig)
GenEMBL U57520(517bp) U57521(495bp)
Choi,I., Collante,W., Simmen,R.C.M., Troyer,D. and Simmen,F.A.
Molecular cloning and structural characterization of porcine
cytochrome p450 aromatase chromosomal genes: evidence for the
existence of multiple, closely related genes that encode
developmental and tissue-specific isoforms of aromatase.
unpublished (1997)
CYP19 bovine
GenEMBL S66248 (2104bp)
Hinshelwood,M.M., Corbin,C.J., Tsang,P.C. and Simpson,E.R.
Isolation and characterization of a cDNA insert encoding bovine
aromatase
cytochrome P450.
Endocrinology 133, 1971-1977 (1993)
Note two amino acid differences with Z32741
CYP19 bovine
GenEMBL M64646 (1725bp)
Zuber,M.X., John,M.E., Okamura,T., Simpson,E.R. and Waterman,M.R.
Bovine adrenocortical cytochrome P-450-17-alpha: Regulation of gene
expression by ACTH and elucidation of primary sequence.
J. Biol. Chem. 261, 2475-2482 (1986)
CYP19 bovine
GenEMBL Z32741 (4226bp) PIR S44210 (503 amino acids)
Vanselow,J. and Furbass,R.
Aromatase cytochrome P450 gene and pseudogene
unpublished (1994)
CYP19 bovine
GenEMBL Z69241 to Z69250 (genomic sequences)
Furbass,R. and Vanselow,J.
unpublished (1996)
CYP19P bovine
GenEMBL Z32813 (1006bp)
Vanselow,J. and Furbass,R.
Aromatase cytochrome P450 gene and pseudogene
unpublished (1994)
CYP19A1 Bos taurus (cow)
See cattle page for details
MLLEVLNPRHYNVTSMVSEVVPIASIAILLLTGFLLLVWNYEDTSSIPGPSYFLGIGPLI
SHCRFLWMGIGSACNYYNKMYGEFMRVWVCGEETLIISKSSSMFHVMKHSHYISRFGSKL
GLQFIGMHEKGIIFNNNPALWKAVRPFFTKALSGPGLVRMVTICADSITKHLDRLEEVCN
DLGYVDVLTLMRRIMLDTSNMLFLGIPLDESAIVVKIQGYFDAWQALLLKPDIFFKISWLCRKYEKSV
927 KDLKDAMEILIEEKRHRISTAEKLEDSIDFATELIFAEKRGELTRENVNQC 1079
1080 ILEMLIAAPDTMSVSVFFMLFLIAKHPQVEEAIIREIQTVVGERDIRIDDMQKLKVVEN 1256
1257 FINESMRYQPVVDLVMRKALEDDVIDGYPVKKGTNIILNLGRMHRLEFFPKPNEFTLE 1430
1431 NFAKNVPYRYFQPFGFGPRACAGKYITMVMMKVVLVTLLRR 1553
FHVQTLQGRCVEKMQKKNDLSLHPDETRDRLEMIFTPRNSDKCLER*
CYP19 Equus caballus (horse)
GenEMBL U37313 (458bp)
Choi,I., Simmen,R.C. and Simmen,F.A.
Molecular cloning of cytochrome P450 aromatase complementary
deoxyribonucleic acid from periimplantation porcine and equine
blastocysts identifies multiple novel 5'-untranslated exons
expressed in embryos, endometrium, and placenta.
Endocrinology 137 (4), 1457-1467 (1996)
CYP19 chicken (three different strains of chicken)
GenEMBL M73277 to M73285, M73286 to M73294
M73295 to M73303
PIR A41063 (495 amino acids)
Matsumine,H., Herbst,M., Ou,S.-H.I., Wilson,J.D. and
McPhaul,M.J.
Aromatase mRNA in the extragonadal tissues of chickens with
the henny-feathering trait is derived from a distinctive
promoter structure that contains a segment of a retroviral
long terminal repeat.
J. Biol. Chem. 266, 19900-19907 (1991)
CYP19 Coturnix coturnix japonica (Japanese quail)
GenEMBL S46949 (692bp) PIR A48977(230 amino acids)
Harada,N., Yamada,K., Foidart,A. and Balthazart,J.
Regulation of aromatase cytochrome P-450 (estrogen synthetase)
transcripts in the quail brain by testosterone.
Brain Res. Mol. Brain Res. 15, 19-26 (1992)
Note: only three amino acid differences with chicken.
CYP19 Coturnix coturnix (quail)
GenEMBL D50336(4351bp)
Kudo,T., Yamamoto,H., Sato,S. and Sutou,S.
Comparison of 5' upstream regions of chicken and quail aromatase
gene.
Unpublished (1995)
CYP19 Poephila guttata (zebra finch)
GenEMBL S75898(3188bp)
Shen,P., Campagnoni,C.W., Kampf,K., Schlinger,B.A., Arnold,A.P. and
Campagnoni,A.T.
Isolation and characterization of a zebra finch aromatase cDNA: in
situ hybridization reveals high aromatase expression in brain
Brain Res. Mol. Brain Res. 24 (1-4), 227-237 (1994)
CYP19A1 Xenopus tropicalis
See Xenopus pages for seq
CYP19 Ictalurus punctatus (channel catfish)
GenEMBL S75715(2102bp)
Trant,J.
Isolation and characterization of the cDNA encoding the channel
catfish (Ictalurus punctatus) form of cytochrome P450arom
Gen. Comp. Endocrinol. 95 (2), 155-168 (1994)
CYP19 Onchorynchus mykiss (rainbow trout)
Tanaka, M., Telecky, T.M., Fukada, S., Adachi,S., Chen, S. and
Nagahama, Y.
Cloning and sequence analysis of the cDNA encoding P-450 aromatase
(P450arom) from a rainbow trout (Onchorynchus mykiss) ovary,
relationship
between the amount of P450arom mRNA and the production of
oestradiol-17-beta
in the ovary.
J. Mol. Endocrin. 8, 53-61 (1992)
CYP19 Carassius auratus (goldfish)
GenEMBL U18974(2939bp)
Gelinas,D.M., Pitoc,G.A. and Callard,G.V.
Isolation of goldfish brain aromatase cDNA and analysis of
expression during the reproductive cycle and after steroid
treatment in vivo.
unpublished (1996)
CYP19 Oryzias latipes (medaka)
GenEMBL D82968(1851bp)
Tanaka,M., Fukada,S., Matsuyama,M. and Nagahama,Y.
Structure and promoter analysis of the cytochrome P-450 aromatase
gene of the teleost fish, medaka (Oryzias latipes)
J. Biochem. 117 (4), 719-725 (1995)
CYP19 Tilapia nilotica (Cichlid fish)
GenEMBL U72071(1804bp)
Chang,X.T., Kobayashi,T., Nakamura,M., Kajura,H. and Nagahama,Y.
Isolation and characterization of cDNA encoding the tilapia
(Oreochromis niloticus) cytochrome P450 aromatase (P450arom):
Changes in P450arom mRNA, protein and enzyme activity in ovarian
follicles during oogenesis.
J. Mol. Endocrinol. (1996) In press
CYP19 Haplochromis burtoni (cichlid fish)
GenEMBL AF114716
White,R.B.
Aromatase expression during social change in the cichlid fish,
Haplochromis burtoni.
Unpublished
CYP19 Paralichthys olivaceus (Japanese Flounder)
GenEMBL AB017182
Kitano,T., Takamune,K., Kobayashi,T., Nagahama,Y. and Abe,S.
Suppression of P450 Aromatase (P450arom) Gene Expression in
Sex-Reversed Males Produced by Rearing Genetically Female Larvae at
High Water Temperature during a period of Sex Differentiation in
Japanese Flounder (Paralichthys olivaceus)
Unpublished (1998)
CYP19A1 Fugu rubripes (pufferfish)
No accession number
Scaffold_7098
65% to AF183906 ovary form of CYP19 from Zebrafish
60% to AF183908 brain form of CYP19 from Zebrafish
61% to other Fugu CYP19
this is probably the ovary form
9466 MAAVGLDAEVLVSVSPNATEAESPGSSAGTRALIILTCLLLLVWSHTEKKSVP 9308 (1)
9242 SLLGPSFCLGFGPLLTYVRFIWTGIGTASNYYNKKYGDIVRVWVNGEETLVISR 9081 (2)
8985 ASAVHHVLKSRQYTSRFGSKQGLSCIGMNERGIIFNNNVTEWRKIRGYFTK 8830 (1)
8759 ALTGPAVQNTVEVCNSSTQAHLDRLEDLAQVDVLSLLRCTVVDISNRLFLDIPIN 8595 (1)
8499 EKELLLKIHKYFDTWQTVLIKPDIYFKFGWIHQKHKTAA 8392 (2)
8296 RELQEAIEGLVEQKRRDLEQADKLENINFTAELLFAQ 8186 (0)
8084 NHGELSAENVMQCVLEMVIAAPDTLSVSLFFMLLLLKQNPDVELQLLQEIDAVVGK (0 expected, bad boundary)
RQLQNGDLQKLRVLETFINECLRFHPV 7719
7718 VDFTMRRSLSDDVIEGYRVPKGTNIILNTGHMHRTEFFLRPTEFCLQNFEKN 7563 (0)
APRRYFQPFGSGPRACVGKHIAMVMMKSILVTLLSQYSVCPHEGLT 7327
7326 LDCLPQTNNLSQQPVEHQEEAQQLSMRFLPRQRGSWQTV* 7207
CYP19A1 Danio rerio (zebrafish)
GenEMBL AF183906
Chiang,E.F.L., Yan,Y.L., Guiguen,Y., Postlethwait,J. and Chung,B.C.
Two Cyp19 (P450 Aromatase) Genes on Duplicated Zebrafish
Chromosomes Are Expressed in Ovary or Brain
Mol. Biol. Evol. 18 (4), 542-550 (2001)
Called CYP19a expressed in ovary
CYP19A2 Fugu rubripes (pufferfish)
No accession number
Scaffold_4200
65% to both brain and ovary forms of CYP19 from Zebrafish
61% to other Fugu CYP19
this is probably the brain form
2918 MKPKETLNITASGPFTPLPLPLMMMMMMLLLMMMMLFLTWNRPQRQHVP (1)
3451 GPLFLAGLGPLLSYCRFMWTGIGTACNFYNNKYGSLVRVWINGEETLILSR (2)
3674 SSAVYHVLRSAHYTARFGSRAGLECIGMEGQGVIFNSDVQLWRRARVYFSK (1)
3960 ALTGPGLQRTVGVCVTSTAKHLDCLVDMTDASGHVDALNLLRAIVVDISNRLFLRVPLN 4136 (1)
EKDLLTKIHNYFETWQAVLIKPDIFFKIGWLFDKHRRAA 4319 (2)
QELQDTMAALLKVKRKLVHEAEKLDDVLDFATELILAQ (0)
EAGEFSADNVRQCVLEMVIAAPDTLSISLFFMLMLLKQHPDVELRIVEELSTvsrt (0)
egeENIDYQRLKVMESFINESMRFHPVVDFTMRKALEDDTIEGIRIRKGTNIILNIGLMHKTE 5039
5040 FFPKPREFSLTNFEQT (0)
VPSRFFQPFGCGPRSCVG 5219
5220 KHIAMVMMKAILATLLSRYTVCPRHGCTLTSIRQTNNLSQQPVEDEHSLAMRFIPRTIQSPS* 5408
CYP19A2 Danio rerio (zebrafish)
GenEMBL AF183908
Chiang,E.F.L., Yan,Y.L., Guiguen,Y., Postlethwait,J. and Chung,B.C.
Two Cyp19 (P450 Aromatase) Genes on Duplicated Zebrafish
Chromosomes Are Expressed in Ovary or Brain
Mol. Biol. Evol. 18 (4), 542-550 (2001)
Called CYP19b expressed in brain
CYP19 Trachemys scripta (red-eared slider turtle)
no accession number
Crews,D.
Temperature-dependent sex determination: the interplay of steroid
hormones
and temperature.
Zool. Sci. 13, 1-13 (1996)
CYP20 human
GenEMBL AC011737.8 chromosome 2 clone RP11-33N4,
AC011737.8 chr 2 (missing exons 12,13)
AC080075.2 (missing exons 1,7,8)
MLDFAIFAVTFLLALVGAVLYLYP (0)
ASRQAAGIPGITPTEEK (2)
DGNLPDIVNSGSLHEFLVNLHERYGPVVSFWFGRRLVVSLGTVDVLKQHINPNKTS (1)
DPFETMLKSLLRYQSGGGSVSENHMRKKLYENGVTDSLKSNFALLLK (0)
LSEELLDKWLSYPETQHVPLSQHMLGFAMKSVTQMVMGSTFEDDQEVIRFQKNHGT (0)
VWSEIGKGFLDGSLDKNMTRKKQYED (1)
ALMQLESVLRNIIKERKGRNFSQHIFIDSLVQGNLNDQQ (0)
ILEDSMIFSLASCIITAK (1)
LCTWAICFLTTSEEVQKKLYEEINQVFGNGPVTPEKIEQLR (2)
YCQHVLCETVRTAKLTPVSAQLQDIEGKIDRFIIPRE (0)
TLVLYALGVVLQDPNTWPSPHK (2) genomic seq stops here the rest is cDNA
FDPDRFDDELVMKTFSSLGFSGTQECPELR (2) intron site based on fish genomic DNA
FAYMVTTVLLSVLVKRLHLLSVEGQVIETKYELVTSSREEAWITVSKRY
CYP20 mouse
GenEMBL AK020848 adult retina cDNA plus ESTs for C-term
MLDFAIFAVTFLLALVGAVLYLYPASRQASGIPGLTPTEEKDGN
LPDIVNSGSLHEFLVNLHERYGPVVSFWFGRRLVVSLGTTDVLKQHFNPNKTSDPFET
MLKSLLGYQSGGGSAGEDHVRRKLYGDAVTASLHSNFPLLLQLSEELLDKWLSYPETQ
HIPLSQHMLGFALKFVTRMVLGSTFEDEQEVIRFQKIHG
TVWSEIGKGFLDGSLDKNTTRKKQYQEALMQLESTLKKIIKERKGGNFRQHT
FIDSLTQGKLNEQQILEDCVVFSLASCIITAR
LCTWTIHFLTTTGEVQKK
LCKEIDQVLGEGPITSEKIEQLSYCQQVLFETVRTAKLTPVSARLQDIEGKVGPFVIPKE 360
TLVLYALGVVLQDPSTWPLPHRFDPDRFADEPVMKVFSSLGFSGTWECPELXFAYMVTAV 540
LVSVLLEKLRLLAVDRQVVEMKYELVTSAREEAWITVSKRH*
CYP20A1 Bos taurus (cow)
See cattle page for details
MLDFAIFAVTFLLALVGAVLYLYPASRQAAGIPGITPTEEKDGN
LPDIVNSGSLHEFLVNLHERYGPVVSFWFGRRLVVSLGTVDVLKQHINPNKTLDPFET
MLKSLLRYQSDSGNVSENHMRKKLYENGVTNCLRSNFALLIKLSEELLDKWLSYPESQ
HVPLCQHMLGFAMKSVTQMVMGSTFEDEQEVIRFQKNHGTVWSEIGKGFLDGSLDKST
TRKKQYEDALMQLESILKKIIKERKGRNFSQHIFIDSLVQGNLNDQQILEDTMIFSLA
SCMITAKLCTWAVCFLTTYEEIQKKLYEEIDQVLGKGPITSEKIEELRYCRQVLCETV
RTAKLTPVSARLQDIEGKIDKFIIPRETLVLYALGVVLQDPGTWSSPYKFDPERFDDE
SVMKTFSLLGFSGTRECPELRFAYMVTAVLLSVLLRRLHLLSVEGQVIETKYELVTSS
KEEAWITVSKRY
CYP20A1 Sus scrofa (miniature pig)
no accession number
Haitao Shang
Submitted to nomenclature committee May 23, 2007
partial sequence
92% to human 20A1, full seq. is not known
Ortholog of human CYP20A1
CYP20A1 Sus scrofa (pig)
DT324969.1, BG894896.1, BI336725.1, BP449309.1
Trace file gnl|ti|861177001
lower case = cow seq.
MLDFAIFAV
TFLLALVGAVLYLYPASRQAAGIPGITPTEEKDGNLPDIVNSGSLHEFLVNLHERYGPVV
SFWFGRRLVVSLGTVDVLKQHINPNKTSDPFETMLKSLLRYQSDSGNMSENHMRKKLYEN
GVTNSLQSNFALLLKLSEELLDKWLAYPESQHVPLCQHMLGFAMKSVTQMVMGSTFEDEQ
EVIRFQKNHGTVWSEIGKGFLDGSLDK
NHMRKKLYENGV
TNCLRSNFALLIKLSEELLDKWLSYPESQ
HVPLCQHMLGFAMKSVTQMVMGSTFEDEQEVIRFQKNHGTVWSEIGKGFLDGSLDKSTTRK
KQYEDALMQLESILKKI ikerkgrnfsqhifidslvqgnlndqqiledtmifsla
scmitaklctwavcflttyeeiqkklyeeidqvlgkgpitsekieelrycrqvlcetv
RTAKLTPVSARLQDIEGKIDKFIIPRETLVLYALGVVLQDPGTWSSPYK
FDPERFDDESVMKTFSLLGFSGPQEC
PELGWRYMVTTVLWSVLLRRRHLLPVEGQVIETNYDLVTSS
XEEAGITVSKRFRPGILLPWLRKLTI*
CYP20 Gallus gallus (chicken)
BU454844 603215388F1 CSEQRBN14 Gallus gallus cDNA clone ChEST201o21 5'.
BU111630 603125978F1 CSEQCHL13 Gallus gallus cDNA clone ChEST95k19 5'
BU356654 603474052F1 CSEQCHN70 Gallus gallus cDNA
MLDFAIFAVTFLLILVGAVLYLYP
ASRQASGIPGLAPTDDK
DGNLPDIIASRSLHEFLVNLHEKYGPLVSFWFGRRLVVSLGSIDLLKQHVNPNRSS
DPFEMMLKSFLRYQSSLNGDTGESHLRRKLYESGVSKSLQSNLALIQK
LSEELLAKWLSLPEAQHIPLCQHMLGFAMKSVTQTAMGSSFEDDQEVIRFRRHHDA
IWSEIGKGFLDGSLDKNATRKKLYED
ALKEMESTLRKVITGTPRQIIQQAFIDTLLQGNLSDQQ
ILEDTMIFSLAGCIITAN
LCTWAVYFLTTSEDVQQNLCKEVDHVLGKGPITHEKIEQLR
YCRQVLCETVRTAKLTPIAAQLQELEGRVDQHTVPKE
TLVLYALGVMLQDSSSWPSPYK
FDPERFSEDSAMTNFSLLGFSGSQECPELR
FAYMVATVLLSILVRKLYLHPVKGQVMETKYELVTSPKEEAWITVSKRS*
CYP20 Xenopus laevis (African clawed frog)
BJ037591.1 NIBB Mochii normalized Xenopus neurula library Xenopus laevis cDNA clone XL040p15 5'
BQ725586 BQ725586.1 AGENCOURT_8103453 NICHD XGC Emb2 Xenopus laevis cDNA clone
95% identical to tropicalis seq. 74% to chicken
BC044111.1 mRNA, complete cds Length = 2417
175 MLDFAIFAITFLLILVGAVLYLYPSSRQACGIPGLAPTEEKDGNLQDIVNSGS 333
334 LHEFLVNLHERFGPVASFWFGRRLVVSLGSLDLLKQHINPNKTSDPFQTMLKSLLGYQSG 513
514 VIGEAAESHVQKKLYENGITKALHSNFSVIIKLSEELLAKWGTYPQSQHVPLCQHMLGFA 693
694 MKSVTQTAMGSSFEDDQEVIHFRRNHDAIWSEIGKGFLDGSIERSPSRKKLYEDALMEME 873
874 TVLKKTIKERKGKNPGRHVFLDSLLQGNLSDKQVLEDSMIFSLAGCVITANLCTWAIYFL 1053
1054 TTSEEVQDKLYKEVNRVIGKGPITMDKLEQLSYCRQILCETVRTASLTPISARLQELEGR 1233
1234 VDQHIIPKETLVLYALGVVLQDNTAWPLAYRFDPDRFDDETAKQSLSLLGLSGSQECPEL 1413
1414 RFAYMVAMVLLCVLVRKLNLLPVKGQVMETKYELVTSPKEEAWITVSKRS 1563
CYP20 Xenopus tropicalis
From JGI blast server http://aluminum.jgi-psf.org/prod/bin/runBlast.pl?db=xenopus1&dump=1
And from Sanger http://www.sanger.ac.uk/Projects/X_tropicalis/blast_server.shtml
AL848968.1 Xenopus tropicalis EST, clone TEgg007o12 5'
AL870093 AL870093.1 Xenopus tropicalis EST, clone TEgg120l22 5'
60% to Fugu CYP20, 70% to human CYP20, 62% to zebrafish, 30% to ciona CYP20 like seq
72% to chicken
MLDFAIFAITFLLILVGAVLYLYP exon 1
SSRQACGIPGLAPTEEK exon 2
DGNLQDIVNSGSLHEFLVNLHERFGPVASFWFGRRLVVSLGSLDLLKQHINPNKTS exon 3
DPFQMMLKSLLGYQSGVIGEAAESHVQKKLFENGIIKALHSNFSVVIK exon 4
LSEDLLAKWLTYPQSQHVPLCQHMLGFAMKSVTQTAMGSSFDDDQEVIHFRRNHDA exon 5
IWSEIGKGFLDGSIERSPNRKKLYED exon 6
ALMEMETVLKKAIKERKVKNPGRHVFVDSLLQGNLSDKQ exon 7
VLEDSMIFSLAGcvitan exon 8
VCTWAIYFLTTSEEVQDKLFKEVTRVIGKGPITMDKLEQLS exon 9
YCRQILCETVRTASLTPISARLQELEGRVDQHIIPKE exon 10
tlvlyalgvvlqdntawplayr exon 11 X. laevis
FDPDRFNDETAKQSLTLLGFSGSQECPELR exon 12
FAYMVAMVLLSVLVRKLHLLPVKGQVMETKYELVTSPKEEAWITVSKRS exon 13
CYP20 Fugu rubripes (pufferfish)
No accession number
Scaffold_486
59% TO CYP20 human
MLDFAIFAVTFVIVLVGAVLYLYP (0)
SSRRASGIPGLNPTDEK (2)
11654 DGNLQDIVGRGSLHEFLVSLHQEFGPVASFWFGSRPVVSLGSLQQLRQHINPNHST 11487 (1)
DSFETMLKSLLGYHSGGGGASTDSIIRKKVYQGAIDTTLKNNFPLVLK (0)
LVDELVGKWKSFPEDQHTPLCAHQLVLAMKTITQLALGESFSEDARVIAFRKNHDV (0)
IWSEIGKGYMDGSLEKSTSRKGHYEK (1)
ALSEMESTLLSVVKERKSQRNKSVFVDSLIQSTLTERQ
IMEDCMVFMLAGCAITAN
VCIWALHFLSTSEEVQDRLYKEFEEVLGSSPVSLEKIPQLR
YCQQVLNETLRTAKLTPIAARLQEVEGKVDQHLIPKE
SLVIYALGVILQDSDTWNAPYR
FDPDRFEEESVKKSFHLLGFSGSQTCPELR
FAYTVATVLLSVLVRQLKLHRLKDTLMEVRSELVSTPRDETWITFNLRN*
CYP20 Tetraodon nigroviridis (freshwater pufferfish)
>FS_CONTIG_2529_2 Length = 13425 from http://fugu.hgmp.mrc.ac.uk/blast/
>FS_CONTIG_2529_1 Length = 1009 from http://fugu.hgmp.mrc.ac.uk/blast/
MLDFAIFAVTFVVILVGAVLYLYP
SSRRASGVPGLNPTDEK
XXXXXXXXXXXDIVARGSVQEFLVSLHQEFGPVASFWFGSRPVVSLGSLQQLQQHANPNRSS
DSFETMLKSLLGYHSGGGGASTENIIRKKVYQGAIDATLKNNFPLVLK
LVDELVGKWTSSPEDQHTPLCAHQLVLAMKSITQLALGESFSQDARVVSFRRNYDA
IWSEVGKGFMDGSLERSTSRKGRYEX
ALSEMEATLLSVVKDRKSQRKTSVLVDTLLQSTLTDRQ
IMEDCMVFTLAGCAITAN
VCIWALHFLSSYEDVQDRLHQELEEVLGSGSVSLEKIPQLR
YCQQVLNETVRTAKLTPVAAGLQEVEGKVDQHLIPKE
TLVIYALGVILQDSHTWDAPCR
FHPDRFEEESVRKSFRLLGFSGSQTCPELR
VAYTVATVLLSAVVRQLRLHRLEDTLVEVRSELVSTPREETWITFSRRN
CYP20 Danio rerio (zebrafish)
Assembled CYP20 from zebrafish ESTs
BQ259821 faa04d08.y1 zebrafish fin day3 regeneration
BM185037 fv16g05.x2 zebrafish adult brain
BG985721 2543 NICHD Zebrafish normalized I
BM070720 fu98e06.y1 zebrafish adult brain cDNA
CA472036 AGENCOURT_10739799 NCI_CGAP_ZKid1 cDNA (kidney?)
BI981894 fu52c12.y1 zebrafish adult brain Danio rerio cDNA
BM083017 fu28a11.y1 Campbell zebrafish ovary cDNA MLDFAIFAVTFVIILIGAVLYLYPSSRRASGVPGLNPTEEKDGNLQDIVNKGSLHEFLVG
LHDEFGSVASFWFGARPVVSLGAVNQLRQHINPNWTTDSFETMLKSLLGYQSGSGVGLTE
SMMRKKVYEGAINKTLENNFPLLLQQVEELVDKWASYPKSQHTPLCAHLLGLAMKAVTQL
AMGSRFRDDAEVIRFRKNHEAIWSQ
IGKGYLDGSLEKSSSRKAHYESALAEMESVLKSVAKQRPGQGSSQSFVNYLLQANLTER
QVMEDGMVFTLAGCVITANLCIWAVHFLSVSEAVQDRLYHELVEVLGDEPVSLEKIPQLR
YCQQVLNETVRTAKLTPVAARLQEVEGKVDQHIIPKETLVIYALGVVLQDADTWSLPYRF
NPDRFAEESVMKSFSLLGFSGSQACPELRFAYTVATVLL
STLVRRLRMHRVDGQVVEARYELVTTPKDDTWITVSKRN*
CYP20 Danio rerio (zebrafish)
>ctg10765 genomic contig CYP20 74% to fugu
9501 MLDFAIFAVTFVIILIGAVLYLYP (0) 9572
SSRRASGVPGLNPTEEK (2?)
DGNLQDIVNKGSLPEFLVGLH
DEFGSVASFWFGARPVVSLGAVNQLRQHINPNWT (1) 10024
12291 TDSFETMLKSLLGYQSGSGVGLTESMMRKKVYEGAINKTLENNFPLLLQ (0) 12439
12929 QVEELVDKWASYPKSQHTPLCAHFL 13003 frameshift
13003 GLAMKAVTQLAMGSRFRDDAEVIRFRKNHEA (0) 13095
15738 IWSEIGKGYLDGSLEKSSSRKAHYES (1?) 15815 AC boundary istead of AG
15897 ALAEMESVLKSVAKQRPGQGSSQSFVNYLLQANLTERQ (0) 16010
16583 VMEDGMVFTLAGCVITAN (1) 16636
17689 LCIWAVHFLSVSEAVQDRLYHELVEVLGDELVSLEKIPQLR (2) 17811
19293 YCQQVLNETVRTAKLTPVAARLQEVEGKVDQHVIPKE (0) 19403
21269 TLVIYALGVVLQDADTWSLPYR (2) 21334
21425 FNPDRFAEESVMKSFSLLGFSGSQACPELR (2) 21514
FAYTVATVLLSTLVRRLRMHRVDGQVVEARYELVTTPKDDTWITVSKRN* from wz10135.3
CYP20 Oryzias latipes (medaka fish)
BJ524824.1 MF01SSB cDNA Oryzias latipes cDNA
BJ003683.1 BJ003683 MF01SSA cDNA Oryzias latipes cDNA
MLDFAIFAVTFVVILVGAVLYLYPSSRRASGVPGLFPTDEKDGNLQDIVDRGSLHEFLV
GLHEQFGPVASFWFGRQPVVSLGSVDPLRQHINPNHTTDSFETMLKSLLGYQAGAGGGAN
ESVMRKKLYESAINNALKNSFPAVLKVAEELVDKWSSVPEDQHIPLCAHLLGLALKTV
TQLALGERFKDDAEVISFRKNHEAIWSEIGKGYMDGSLEKSSSRKRHYE SA
LSDMEATLLAV AKDRKAQRRQTA FVDALLQSGLTERQIMEDCMVFTLAGC
VITANLCIWALHFLSTAEDVQEKLCQEVEDLFGSDPVSLDRIPQLKYCQQVLNE
TVRTAKLTPVAARLXEVEGKVGQHVIPKETLVIYALG
VVLQDADTWSTPYRFDPDRFQDESARKSFCLLGFSGSQTCPELRFAYTVATVLLATLVRRLKLRPLK
About 26 aa missing at end
CYP20 Salmo salar (Atlantic salmon)
GenEMBL CA063128.1 ssalrgb509318 mixed_tissue Salmo salar cDNA.
GenEMBL CB516811.1 ssalrgb509318_rev mixed_tissue Salmo salar cDNA.
GenEMBL CB513409.1 ssalrgb531212_rev mixed_tissue Salmo salar cDNA.
BG935303.1 SL1-0624 Atlantic Salmon liver Salmo salar cDNA clone SL1-0624
MLDFAIFAVTFVIFLVGAVLYLYPSSRSASGIPGLNPTEEKDGNLQDIVNRGSLHEF
LASLHGQFGPVASFWFGGRPVVSLGSVDQLRQHINPNRTTDSFETMLKSLLGYQSGTGGG
ATEAVMRKKLYESAVNNTLEKNFPMLLKLVEELVGKWQSFPKDQHTPLCAHLLGLAMKAVTQ
18 amino acids missing here
RKNHEAIWSEIGKGYLDGSMEKSSIRKEHYESA
LAEMETVLMSVAKDRKGQRSQTAFVDTLLQSNLTERQVME
DSMVFTLAGCVITANLCIWAVHFLST
SEDVQEKLHQELEDVLGSEPVSLDK IPQLRYFQQVLNETVRTAKLTPIAARLQENEGKVD
QHIIPKETLVIYALGVVLQDADTWSCPYKFDPDRFTEDSARKSFSLLGFSGNQACPELRF
AYTVATVVLSTVVRQLKLYQVKGQVVEARSELVSTPKDDTWITVSRRS*
CYP20 Ciona intestinalis
SEQUENCE 186, 187 CYP20 ortholog
29% to fugu CYP20 CYS = HIS
MLEVHVTITAVAVLAVILLLFLIGLLFYIYPSSN (?)
KETTVPGPAVSDIR (2)
DGNRSDIDRAGSLHQYLCNLHAEHGDLASFHFDKQLIISIADSKYFAPRSQVFQLP (1)
DFLYEFWEPMIGKESFVFSQGVDVFKRRDNIDFKVDKKVKEFYAKIF (0)
RLLAIELEEKWRKQPEDQHIPLTQHLKGLATKAVCQMMFGESFADDERVIKLYKAWDK (0)
CWNEVMAASKMEPTKSESDEKK (1)
SIKELRSLMEKAIEERWKKSKHLDGITLLDGLLASSNSSVSPLSSEQLLCDVITMFTMGVQVTNA (1)
VLCWSVYFIAAHDDVQTKLRNKLNEVESLDIMPQSLNDVK (2)
YMQSILKESIRCSGVFSYSGRVEDIDITVGDHLLPKG (0)
TPIIEALMFPMQSDAFESPDM (2)
FNPKNFSEATKSVNPLTYIPFAFGGSRHKPQDS (2)
LVYLLVSITVASLFRNLRISLAGKEVDPQSANYESFVALPSTEEVWVTVGGKLDVETVGENN*
CYP20 Ciona savignyi
sequence for CYP20 ortholog
72% to C. intestinalis seq 186
MLEVHVSITAVAILVLIILLFLIGLLVYIYPVI (2)
KETTVPGPDVSDAK (2)
DGNKSDIERAGSLHAYLCNLHADFKHEGAVSFHHGKQLVISISNPKFFAPRSQVFQLP (1)
SFLYEYWRPLIGEESFLFAKDIDVFRRRDYLDFKVNRNVKEFYAKNF (0)
LAVELETKWEKQPEDQHIPLTQHLKGLATKAVCQMMFGDNYADGTKVLELYRAWDK (0)
CWNEVMAASKMEPTKSESVEKK (1)
SISELRKLMENAIEERWNKSKSLDGITLLDGLLSSQTSDSKDQLTSEQLLCDVITMFTMGVQITTA (1)
VLCWSCYFVASNKEVQRKLKNQLNESKSLDIMPETLMDME (2)
YMQGILKESVRCSGMFSFAGRVEDIDITVQNHLIPKG (0)
TPILEAIMTEMRSEAFSCPDV (2)
FNPKNFTKDNNHLNPLTFIPFAFGGSRHKPEES (2)
LVYLLAAVTVSSLFRNLQVSLAGKEIEPFAANFESFVAMPSTEEIWVTVSKEKEVENVG*
CYP20 Halocynthia roretzi (tunicate, Urochordate)
AV385510
51% to CYP20 in Ciona
KYMKMVLKESLRSGCVFSWAGRQQDIDINVGGNLITKYTPVIEAIGTAMTDEGNFSDPSE
FDPKRFSEDNDEVINPLDYIPFGFGGSRKAPQNSLVFLLASIVASTLIRNLKVCIAGDSS
QNQPDPDFSAFVSRPSKEELWVTVSALGKKKAETVG*
CYP20 Molgula tectiformis (tailless ascidian, tunicate, Urochordate)
CJ366548.1 EST
N-term
MDVQITLATLGVFIVLFVLFLLGFLFYLYPGSNNQSTIPGPNPSDAKDGNVSDLQ
RVGSLQKYLEELHSEYGDIIAFWHQSNQIVSIADLDEFAQTNQVFERPDILFSSWIPLIG
KDSFIFEKDVKNFQRLDML
CYP20 Branchiostoma floridae (amphioxus)
There are 8 CYP20s in amphioxus (see the amphioxus page)
CYP20 Saccoglossus kowalevskii (Acorn worm, hemichordate)
FF488963, FF488605, FF525072, FF503042, FF480950, FF429742
38% to Paracentrotus lividus, 30% to CYP20 human
33% to sponge CYP38A1
MTALVIIVVVLLVVVVAVFYLGTLFGSKQEQMLARIPGMNPTNSED
GNMPDITVAGSLHEFLVKLHTKYGHVATFWYGKTHVISLASAKAFKDTNKLFDRPAVLFNFVKPLIGPD
SIQYANEEDGKRRRHDYDQSFSYDAIKNYYSKFYEATDAVVAKIESLPSDEHINITEHMS
LLVLKALSHATYGDFFKDDEFSITMLRHYETAMAILNQDISKDTEAGQ
RFAQALKTWHDFIRAMIQHRRDNPPNDDDWTFIDVLMAKSSSEEELISDATSYF
IAGYHTTAFMMVWTFYYMCENQEVQEKIYQEIIEVIGKDEQVNYVNLESLKYMRCVFD
ESMRCSVLAPFAARVNMHADMVVQGYTIPKGTPVVHALGVVLMDDEIWSEPERFIPERFL
PXNVSTRHPLSFQPFGFAGKRKCPGYRIAYAEGAVFLATLCRK
FKFQLVEGQTIERKYGFVTIPSNDIWVTVTKRD*
CYP20A2 Saccoglossus kowalevskii (Acorn worm, hemichordate)
FF425228.1
45% to CYP20A1 Saccoglossus
N-term region
FFQFVKPLFGENSIQFANGNYGKQRRKLHDASFTHQAVKSYYPVFQEKTEIFIRKIMAPP
PEEHIPLSEYVSVFVMKSLMSALFGQNVENDEQTVTMLRHYETVMLVLNKKMSANTEKKN
TFHIALNAWHEFIRKIILKRRNEPSDE
CYP20 Strongylocentrotus purpuratus (purple sea urchin)
GenEMBL XM_787803.1, XM_793215.1
AAGJ02125342.1 (middle piece, two exons that were missing)
85% to Paracentrotus lividus
37% to CYP20 Saccoglossus kowalevskii (acorn worm)
35% to sponge CYP38A1
MLDFAIFAVTFIILLVGLLIYIYP (0)
TTPQKTTTVPGLEPSDPV (2)
KGNLDEIGDAGSLHQFLTKLHAEHGDIASFYFTDQLCVSITSPELFKEHQAVFNRP (1)
ALLFKLFEPLITPDSIQYANGGDGRKRRDLTDRCFGFQALQNFIGVFNK (0)
ITEQLVKKISALPPGEHISLHGTGLGLAIKGIMLTSFGDYFQDDKSITSFRKDYDF (0)
VWGEMEARLDGNMPDEGSNRVKTFEE (1)
1967 ARERMYATIQAVIKFRRSNPPSQDKEVFIDVLLKADYPPGRLQADLLTYVIGGFHTSGN (1) 1791
359 LFAWALFFLAEDDKIQEKLYNHVIDIVGKTGEVSMEDIAEMT (2) 234
YMRQVIDETLRVSILAPYAARYQDFDVVLGGHVIPKG (0)
TPVIHALGVSLQSEKYFPNPKT (2)
FNPENFSAANVKKRPRDAYHPFGFAGRRVCPGQQ (2)
FAYKEVAIFLAVFIRAFKVKVVPGQKPEHVHGLVSHLVNKDGGEVWITVEKR*
CYP20 Paracentrotus lividus (common urchin, Deuterostomes)
AM536943, AM531351, AM584793,
AM534834, AM554260
85% to CYP20 Strongylocentrotus purpuratus
32% to human CYP20 (best match in human) first 11 aa identical
37% to sponge CYP38A1
CYP20 has a highly conserved N-terminal
MLDFAIFAVTFVLLLVGLLIYIYPTTPQKSTTVPGLEASDPVKGNLDEIGDAGSMHQFLTKLHED
HGDIASFYFTDQLCVSIASPELFKEHQAVFDRPPLLFKLFEPLITPDSIQYANGSDGRKR
RDLTDRCFGFQALQNFIGIFNKITEQLVEKISALPPGEHVPLYSTGLGLAIKGIMKTSFG
DYFKDDKSINSFRRDYDYVWAEMEARLDGSMPEPES
EREKKFDEARERLYDTIRGVIKVRRSNPPSQDKEVFIDVLLKADYPPGKLLADL
VSYSVGGFHTSGNLFAWALYFLAQDEEIQEKLYKHVIDIVGKTDEVGMEHIAKMTYMR
QVIDETLRVSILAPYAARFQDFDVVLGGHVIPKGTPVVHALGVSLQSEKYFPNVKVFNPE
NFSPANVKKRPRDAFQPFGFAGRRVCPGQQYAYKEVSI
FLAVFLRAFKIKLVPGQKTEHVHGLVSHLVSKDGGDVWFTVEKR*
CYP20 Rhipicephalus appendiculatus (brown ear tick, arthropod, arachnid,
ecdysozoa)
CD784670.1
35% to CYP20 Danio, 39% to CYP20 human
N-term
MLDFAIFAVCFVVFLLALVLYLYPSSAKQTTIPGLEPSDKKEG
NVGDIVQAGGLQNFLISLHKEHGPIASFWIGTKLVVSIGKADLFKTQSHVFDKPAELFVL
YRDVMGAGSIFFANGAEARKRRRLIDEVLTGKSLDKFLGPIEKLCSEVVMHLKDTPDDEH
VPVYQYMYALCMKISTRLLFGEYFFDDMEVLKFSRNFELCIKELEE
CYP20 Ixodes scapularis (blacklegged tick, arthropod, arachnid, ecdysozoa)
EW872941.1
EW945756.1
N and C-term with a gap in the middle
MLEFAIFAVCFVVFLLMLVVYLYPGSSKQT
TIPGIDPSDPKEGNVGDIVQAGGLKNFLLSLHKDHGPITSFWMGTKLVISIGDAELFKTQ
THIFDKPADLFELYRDLVGADSILFANGAEARQRRRRLDDVLTGDRINDFVGPLQKMCAE
VVDRWESLPAEEHVPIYQYMYALCMKTCTRLLFGDYFFDDKHVLSSAGPLSCVSVLQRCC
ACPRPDSLDQSMR
MTWALYFLATH
LKLQDFMQLSNLQKTIKETLRTATVEPWTARCQDVDVEIAGHVIPKKTPVIQALGVVLHE
EDHWKVPHRFDSSRFNEKFPELAYCPFGFAGMRLCPGKDLSVLHMSVFLVTLCHRL
KLRLIQDQVVTPTFALITKTDDEVWLTLERRRP*
CYP20 Amblyomma cajennense (tick)
EC780049.1 EST
N-term
MLDFAIFAVCFVVFLVALVVYLYPSSTKQTTIPGLGPSDAKEGNVGDIVQA
GGLQNFLVSFYKEHGPIASFWIGTKLVVSIGQGELFKTQSHVFDKPADLFVLYREVMGTG
SIFFANGAEARKRRKLIDDVLTGQRLDKFTLPIEKLCSEVVA
CYP20 Helobdella robusta (leech, annelid worm)
JGI gene model fgenesh4_pg.C_scaffold_12000405 [Helro1:168041]
Helro1/scaffold_12:3739808-3743927
28% to CYP20 Saccoglossus kowalevskii,
29% to Strongylocentrotus purpuratus CYP20,
30% to Paracentrotus lividus CYP20
33% to Amphioxus CYP20
MLDYLIFAATVVVFLILAVIYLYPGSKKSIDIPGPEPVDPLEGNYPDIKACGSLHEFLVDLHETYGPICS
FWAGPQLCVSVASPELFSEQANSFNRPVELFKAQTLLWGENSPFVLNGAEGRKKHAFLTGIFNEKYASCS
LKLEHEILKKQTASWLKWPNEQFFPLYEKISGFVVKFLLSSHFGYDAENSKDVNVIKGNLDNVWQEVGHY
MDDTSDADGLRDIRFEQYKKKLKSFISDALILRKTLPTVSDPAKLPIDHVLQLSEEEAVSILLLLFTNAY
MKYCSLFTWMGYFLALEPHVQDQVRKELGSIPIEEINLANCNEFKYFKKVFQETLRCANIVSHTARIQNT
DIVIQNSKIPAN (0)
TPVIQSLGVVSSNPTIFHEPDK (2)
FNPDRHASKNTPCSSFAS
3747683 QYFGFAGKRICPASSIVMSQAALFFAHVLECFSLTLAPDQVVSSHYGLFA 3747832
3747833 KPKEEIWLSV 3747862
ENINVRRVS*
CYP20 Haementeria depressa (leech, annelid worm)
CN807321.1 EST
67% to CYP20 Helobdella robusta
NAYLKYSSLLTWMGYFLALEPDVQHKLRKELGLVSVSDLSSVNINKFKYFQNTLKETLRC
AKIVPYTARIHNTDIVIKKDKIPANTPIIHALGVVSSDPAIFPEPDEFNPERFDSKNKPN
TFACQSFGFAGKRKCPASSTVASHAALFYSHLLDCFELKLAPDQIVSSHHGLNAKPKEEI
WLSLEM
CYP20 Hirudo medicinalis (medical leech, annelid worm)
EY484183.1 EST
VEADKLNMQDMXRLDYLQLVTKEVLRCAGIVPFTARCQNTDSQLESHRIPAG (0)
TPVLHAIGVVSNDSQIFDNPEE ()
FNPERFKNVNPSQFAFHPFGFAGQRTCPGSQVAIAQVATFYAHVLRSFELELAPNQ
LAAPNHGFYVRPKEEVWLTLKARL*
CYP20 Eisenia fetida (earthworm, annelid worm)
EH670454.1, EH670439.1 ESTs
ANKMKYLNQVLKETLRVARIATFAARVHHSDVVLGGYQVPAEMPILHAIGLSLHDEEVFP
HPLKFDPDRFSVENEKHLPPFSFEPFGFSGKRKCPGYNFSYAEATVYLSLLIRSFKIELA
FDQVAESKFGFVTKIKDDLWVIARPM*
CYP20A1 Capitella sp. I (polychaete worm, annelid)
JGI gene model e_gw1.28.81.1 [Capca1:145552]
+ e_gw1.28.94.1 [Capca1:145544]
Capca1/scaffold_28:376236-381170
And Capca1/scaffold_28:374918-376952
upstream region with N-term
45% to Amphioxus CYP20, 41% to Strongylocentrotus purpuratus CYP20
34% to Helobdella robusta CYP20
MIQFLLFAVFFLVVLVVTVIYLYSGSGSRKSTIPGPEPLDDLEGNNAD
IKASGSLHEFLMDIHADYGPMASFLFGPQLVVSICCPDLYKQYLKTFDRPASLFEFLEPLITANSIQFAN
GAEGRMRHSAYMRSFGDSMVKNYYPMLQ KFASDLTRSLQLEAKETHIPLRKYMFAFAIKAMLRGAYGKYF
DSDEEIHKLRAAYDVCWTDLEHRLLGDLPVEGSKRDERFEKNKKIMYDIMQKVLDDRRSNPPENDERCFV
DALIDLDVSEDVKKSDMLTVLIAGFHTTGLLLTWCLYYLAKHEEIQDKLLGEIEAVLGKDGVVKADN VHQ
LPYLRQVLNESLRCSVLAPFAARVQSTDVEIGGHTIPANTPVLMALGVTLCNDEFYPDPEIFDPERFTEE
NMKNRPSLAFEPFGFGKRKCLGYKFSMAEFSVLIPTLIRHLKVELVPGQVVTPMFGLVTTPTDEIWVTVS
ERK*
CYP20A1P Capitella sp. I (polychaete worm, annelid, Lophotrochozoa)
JGI gene model fgenesh1_pg.C_scaffold_1378000002 [Capca1:185650]
Capca1/scaffold_1378:17781-19064
Pseudogene
51% to the N-term and
72% to the C-term of the whole gene in Capitella
& = frameshift
MIPIIYLLVVILVVIVLAALFVQAGNSQIRKSTIPGPKPRNKHEGNLADIRAIGSLHQFLLNLHGNFGPI
ASFHYGAQMVVSISSPDLYKKYLKTFDRPPVLFEFPMISKHSLLLTNGQDAHIRREAYLHTFSDVAIKQQ
YPMLQG
(deletion of 179 aa)
LHQIP&
FLRQVMNESVRCSVLGAVAGRVQAVDVEVGGHQIPAH (0)
TPVVMAIGVTHVNEEFYPAPERFDPGRFTEENVKSRPSLSFNPFGFGKRKCLGYKFSNAEFYVF
ITTLLRCLKVELVPGQVVTPLYRVVTTPADEVWVTLSPRY*
CYP20 Lottia gigantea
jgi|Lotgi1|82740|gw1.84.105.1
37% to CYP20 Danio
EST FC700835.1
MLDFAIFAVTFFMMLVGAVIYLMPSSRKATSVPGLDPTTKEDGNLSDIGRAGSLHEF
LLDLHGRFGDIASFWMGRQLVISIASPELFKQHQNVFDRPVELFKVFEPVIGERSIQYCN
GNDGRARRQTYDKSFSHHELFSYSPKFQEVADNLVKTLENKVKEEHLSLNQYMIAYAFKT
VMNSLYGDAMDDEKDIIAMRGHYDIMWSEMEKRIADPTVPEDGSPRAISLNLALKEMRKI
VQNIIDFRKKNGKKSEDYLLIDRIVEHMKTEDGIFSEILTFL IGGFHTAGNLLTWAVYFL
ATHEEVEKKLLKEIKNVLGDDDISADNYDQLKYTKQIIDETMRCAVVAPWAARYQDFDSE
LGGHKIPKKTPVIHALGVSLQDERQWPQPKIFDPERFNEENIKKRPHLAFSPFGFAGRRV
CPGRKFAYLESAICLVSLFRRFKFHLVEGQVVQPVFGLVTHSEDDIWVTVSKR*
CYP20 Aplysia californica (Califonia sea hare,mollusc, Lophotrochozoa)
EB277115.1, EB214176.1, EB282241.1
MLDFVIFAVTFLTVLLVAIIWLYPSSKKVTTIPGVDPSSK
EDGNFSDITRAGSLHEFLLDLHSQFGDIAGFWWGQTYVVSIASPELFKAYANVFDRPTELFRF
IEPLISMQSLQFTNGSDGRMRRKAYDRPFSHENLKKHFAEFQTIAGIVEQKWSKKSESDH
IPLGEDMALFSVRATLLTLMGDSFQ
(gap)
VGALHTSAYMLTWAMYFLATH
xxxxxxxxxVQVLGQKDVVTDSNYGQLRYLYQVLQETLRCAVVAPWGARVQDFDTELGGHKVPTK
TPVIHALGVVLQNEKLCPLPKRFDPDRFSTENSKDQPTYAFSPFGFAG
KRTCPGYKFANRGSTVLIATLIKKFEVSMWSDQVVKPVFGLVTRPHEEILLKVSRRK*
CYP20 Crassostrea virginica (Oyster, mollusc, Lophotrochozoa)
EH649216.1 N-term
MLDFVIFAVTFVVALLIAVIYLYPGSKRITTIPGPDLTTKED
GNLADLARAGSLHEYLLDLHAQYGDITAFWMGQELVVSICSPDLFKQHSTVFDRPVSLFK
MFEPLIGEKSIQYANKADGRNRRKLYDPVLSSDYLHHYYTAMQEVSDSLVKKWSSLPKEE
HVPVAQYMIAQALKMA
CYP20 Mytilus californianus (mussel, mollusc, Lophotrochozoa)
ES406577.1 EST
EGGARFQDF
DSELGGHKIPKNTPVIHALGVTQQDEAIWPVPNRFDPDRFSEENSKKRQSLAFQPFGFAG
KRICPGYRFAYAEATVCFVTLLRKFKFNMVEGQVITPVHGLVAHPSEEVWITIDKR*
CYP20 Nematostella vectensis (starlet sea anemone, Cnidarian)
XM_001634458
matches genomic seq NZ_ABAV01008690.1 and ABAV01008690.1
40% to human
31% to CYP20 HUMAN
missing N-term
14466 (2) aga NGNLLDVKKAGSLHEYLLLLHKTYGPVASFMWGTINVVSVADPTLWKE
LRSLFDRP (1) 14639
15646 PELFKLFEPLIGKKSIQYANGVHGRKLRQLFDRGFSHQAVKDYYQ (2) SFIE(0) 15792
15931 (2) DCCSDWFFLCPQVAEKVANQWVIKSSDEHIPIVESMLMLAIRAIGKAGM
GKRFSAEKDVQAFMNAYRT (0) 16134
17805 TWEEMEACLNGSLSTKGSKRDQRFQN (1) 17882
18216 DMEVMRKMIRETISARRNQRTSSPEPLFIDSVLDCDFIDEKEVRK 18350
18790 EDDMISFFVGGFHTTGN (1) 18840
19760 MMSWCFYYLAIHPEIQEKVHQELIDVLGNDAIDPQAASELQ (2) 19882
20039 FCRQVLDETLRCAVVAPWGARVQYDHDLQIGEYVVPKG (0) 20152
20626 TPIITPFGVVLQDPELYPEPQM (2) 20691
21349 FDPDRFSPEAMKELPSHAYEP 21411
21412 FGFAGKRKCPGWRFSIAEGLVFLSVFVRRFKIRLVPGQKVEPVYGLVTTPKEELWVAVEKRK* 21600
CYP20 Hydra magnipapillata (hydra, Cnidarian)
BP508840 BP508840 ESTs
ABRM01022129.1 ABRM01019337.1 genomic
36% to CYP20 C-term
7448 aga LGNLGSLTFDGGIHKFLVENHKRLGPMFSFYWGKELAVSLACPILFKEVATLFNRP (1) 7615
9076 VNQFKLFLPLIGKECVQYANEDRGKKLRQINDRHFSHSSIKSYFKEFKQ (0) 9222
YFLLKVGAELCSKWSSSENTRIPLNQNMLSIAFKTIVKTSFG
agt YTKQVIDEVMRIAVLAPYAARYSDYDIIVDGHLIPKK (0)
TPIILALGTVFQDETIFPEPDRFDPDRFSDKQIEERSALAFQPFGF
AGKRKCPGYRLAYAETLTYTFYIIKNFHISLFDKQSVKMHYGFVTKPSEEIWIKVLRRKNI*
CYP20/CYP38A1 Suberite domuncula (sponge)
GenEMBL Y17816 (1789bp)
Mueller,W.E.G., Wiens,M., Batel,R., Steffen,R., Borojevic,R. and
Custodio,M.R.
Establishment of a primary cell culture from a sponge: Primmorphs
from Suberites domuncula
Mar. Ecol. Prog. Ser. In press
Name change to CYP20 is recommended
MLDFVIFAITAVAGLIGILLFFYFSRSTETKPVSSASPTSTIPR
WSAPPADIEKGDLDVMMKKHGSLHQFLLHLHDNGKTPVTSFWWGKTHVVSFCSPQAFK
ESAVFVNRPVELFVGFEPLITPFSIQYANDEDWVQRSKCLYHTLKGDDLKSYFHHFVQ
IAQEEESLWSSYTSDKEVSLTKEVFPMTIKGIARTCFGDIFKDENELSKMAESYHVCW
RTMEEGVPEAGSKRETEFLKHRRVLEDIIRRIIQERKEGEDLQELPFIDSMLQNYDSE
DKIIADAISFMVGGFHTSGYMFTWMLWYLSSHPESQDRLRTEIERETGGERGDRLKEY
SLRADTFLRQVQDETIRLSTLAPWAARYSDKKVTVCGYTIPAKTPMIHALGVGLKNKT
VWENTDSWDPDRFSPNGRRGNDFCPFGVHSRRKCPGYLFSYFEVGVFASILLSRFEIV
PVEGQTVIQVHGLVTEPKDDIKIYIRSRKED
CYP20 Schmidtea mediterranea (freshwater planarian)
NZ_AAWT01057969
34% to Paracentrotus lividus
33% to Strongylocentrotus purpuratus
32% to Saccoglossus kowalevskii
25% to human CYP20
from SmedGD database
Missing first exon
23008(2)aga KPKQIVIIPGLQKSDPK(2)
22918FGNLLEIKNSGSLHQFLSDLHLKFGAVATFYWNDQKIVSVCSNDLFKSVQDLFDRPSLI
FGNFHEFIGENCLQYANGNEGQNRRKQYDKAFQHDMIIR(2)22625
18726 YISTIENVGKSNLEEWHGSKVTVDSIEHWAYIMAVKSILKACIIPEEQSPEYCDTIITLYKS
VFIGLEKKFVECKVLSDEEEIQFQKDKNQFYSIIREIINKRLDFLENSDLENEKLLLIDY
IILYHRELNRNLIENEVLIYLIGGFHTT(1) 18274
13764(1)GNALSWCIYYLAKHPKCQEKLFTEISKRQIQLQNTSECYKLILD
LKYLKACIEETLRLSQLAPYAARVSDEDRYLSNYEVPANTPIILALGVSLKDEKI
FNNPDQFNPERFVDSNFPSFAFVPFGFAGKRKCPGER(2)13357
13304 FSYLELSIWLIQLVGKYRFTLTNPHQTVNKVYGLVTRMSENVSVQFGERSDVL*13143
21A Subfamily
CYP21A1P human
GenEMBL M13935 (3206bp)
White,P.C., New,M.I. and Dupont,B.
Structure of human steroid 21-hydroxylase genes.
Proc. Natl. Acad. Sci. U.S.A. 83, 5111-5115 (1986)
97% to 21A2 NT_033167.1|Hs6_33343
329415 MLLLGLLLLLPLLAGARLLWNWWKLRSLHLLPLAPGFLHLLQPDLPIYLLGLTQKFGPIYRLHLGLQ 329215
329117 DVVVLNSKRTIEEAMVKKWADFAGRPEPLTCK 329022
LVSKNYPDLSL
328702 XXWSLLWKAHKKLTRSALLLGIRDSMEPVVEQLTQEFCE 328592
RMRAQPGTPVAIEEEFSLLTCSINCYLTFGDKIK 328383
328294 EDNLMPAYYKCIQEVLKTWSHWSIQIVDVIPFLR 328193
328091 FFPNPGLRRLKQAIEKRDHNEEKQLRQHK
327835 ESLVAGQWRDMMDYMLQGVAQPSMEEGSGQLLEGHLHMAAVDLLIGGTETTANTLSWAVV
327654 FLLHHPE 327634
IQQRL*EELDHELGPGASSSRVPYKDRARLPLLNATIAEVLRLWPVV
327292 PLALPHRTTRPS 327257
SISGYDIPEGTVIIPNLQGAHLDETVWERPHEFWP 327069
326971 DRFLEPGKNSRALAFGCGARVCLGEPLARLELFVVLTRLLQAFTLLPSGDALPSLQPLPH
326791 CSVILKMQPFQVRLQPRGMGAHSPGQNQ 326708
CYP21A1P human with congenital adrenal hyperplasia
GenEMBL M26857 (4034bp) X05445
Rodrigues,N.R., Dunham,I., Yu,C.Y., Carroll,M.C.,
Porter,R.R. and Campbell,R.D.
Molecular characterization of the HLA-linked steroid
21-hydroxylase B gene from an individual with congenital
adrenal hyperplasia.
EMBO J. 6, 1653-1661 (1987)
CYP21A1P human
GenEMBL S60612 (426bp)
Collier,S., Tassabehji,M. and Strachan,T.
A de novo pathological point mutation at the 21-hydroxylase locus:
implications for gene conversion in the human genome.
Nature Genetics 3, 260-265 (1993)
CYP21A2 human
GenEMBL M21544 M21545 M21547 to M21550 M23224 M23225
M31022 M31023 (ten segments)
Globerman,H., Amor-Gueret,M., Parker,K.L., New,M.I. and White,P.C.
Nonsense mutation causing steroid 21-hydroxylase deficiency
J. Clin. Invest. 82, 139-144 (1988)
CYP21A2 human
GenEMBL M12792 M23280 (5141bp)
Higashi,Y., Tanae,A., Inoue,H., Hiromasa,T., and
Fujii-Kuriyama,Y.
Aberrant splicing and missense mutations cause steroid 21-
hydroxylase [P-450(C21)] deficiency in humans: possible gene
conversion products.
Proc. Natl. Acad.Sci USA 85, 7486-7490 (1988)
CYP21A2 human
GenEMBL X54940
Partanen,J. and Campbell,R.D.
Rapid characterisation of mutant P450c21 Genes by PCR
unpublished (1993)
CYP21A2 human
GenEMBL X58898 to X58908 PIR S26485 (97 amino acids)
PIR S26484 (371 amino acids) PIR S29670 (495 amino acids)
PIR S29671 (372 amino acids) PIR S26584 (371 amino acids)
PIR S29673 (372 amino acids)
Helmberg,A., Tabarelli,M., Dobler,G. and Kofler,R.
Identification of molecular defects causing congenital adrenal
hyperplasia by cloning of PCR-amplified 21-hydroxylase genes
unpublished (1992)
CYP21A2 human with congenital adrenal hyperplasia
GenEMBL M28548 (4044bp) X05449
Rodrigues,N.R., Dunham,I., Yu,C.Y., Carroll,M.C.,
Porter,R.R. and Campbell,R.D.
Molecular characterization of the HLA-linked steroid
21-hydroxylase B gene from an individual with congenital
adrenal hyperplasia.
EMBO J. 6, 1653-1661 (1987)
Note: mutant gene, 2 amino acid differences
CYP21A2 human with congenital adrenal hyperplasia
GenEMBL M26856 X05448 (4042bp)
Rodrigues,N.R., Dunham,I., Yu,C.Y., Carroll,M.C.,
Porter,R.R. and Campbell,R.D.
Molecular characterization of the HLA-linked steroid
21-hydroxylase B gene from an individual with congenital
adrenal hyperplasia.
EMBO J. 6, 1653-1661 (1987)
Note: normal gene
CYP21A1 Bos taurus (cattle)
GenEMBL M11267, M13545
Chung BC, Matteson KJ, Miller WL. (1986) Structure of a bovine gene
for P-450c21 (steroid 21-hydroxylase) defines a novel cytochrome P-450
gene family. Proc Natl Acad Sci U S A. 83, 4243-4247.
First cattle CYP21A1 gene
CYP21A1 Bos taurus (cow)
See cattle page for details
MVLAGLLLLLTLLSGAHLLWGRWKLRNLHLP
234 PLVPGFLHLLQPNLPIHLLSLTQKLGPVYRLRLGLQEVVVLNSKRTIEEAMIRKWVDFAG 413
414 RPQIPSYKLVSQRCQDISLGDYSLLWKAHKKLTRSALLLGTRSSMEPWVD 563
564 QLTQEFCERMRVQAGAPVTIQKEFSLLTCSIICYLTFGNKEDTLVHAFHDCVQDLMKT 737
738 WDHWSIQILDMVPFLRFFPNPGLWRLKQAIENRDHMVEKQLTRHKESMVAGQWRD 902
903 MTDYMLQGVGRQRVEEGPGQLLEGHVHMSVVDLFIGGTETTASTLSWAVAFLLHHPEIQ 1079
1080 RRLQEELDRELGPGASCSRVTYKDRARLPLLNATIAEVLRLRPVVPLALPHRTTRPSSIF 1259
1260 GYDIPEGMVVIPNLQGAHLDETVWEQPHEFRPDRFLEPGANPSALAFGCGARVC 1421
1422 LGESLARLELFVVLLRLLQAFTLLPPPVGALPSLQPDPYCGVNLKVQPFQVRLQ 1583
PRGVEAGAWESASAQ*
CYP21A1 pig
S53049 M83939
MVLVWLLLLLTLLAGARLLWGQWKLRNLHLPPLVPGFLHLLQPNLPIYLLGLTQRLGPIY
RLRLGLQDVVVLNSKRTIEEALVRKWVDFAGRPQIPSYKLASQHCPDISLGDYSLFWKAH
KKLTRSALLLGVRSSMEPRVEQLTQEFCERMRAQAGTPVTIQKEFSVLTCSIICCLTFGD
KEDTLVHALHDCVQDLMKTWEHWSIQILDMVPFLRFFPSPGLRRLKQAIENRDHLVEKQL
RRHKESMVAGQWRDMLDYMLQEAGRQRVEEGQGQLLEGHVHMSVVDLFIGGTETTANTLS
WAVVYLLHHPEIQWRLQEELDRELGPGAAGSRVPYKDRARLPLLNATIAEVLRLRPVVPL
ALPHRATRPSSIFGYDIPEGTVVIPNLQGAHLDETVWEQPHEFRPDRFLAPGANPSALAF
GCGARVCLGEPLARLELFVVLVQLLQAFTLLPPEGALPSLQPHPHSGINLKVQPFQVRLQ
PRGGRGEGPGPR
CYP21 rat
GenEMBL U56853(1964bp)
Zhou,M.Y., Vila,M.C., Gomez-Sanchez,E.P. and Gomez-Sanchez,C.E.
Cloning of two alternatively spliced 21-hydroxylase cDNAs in the
rat adrenal.
unpublished (1996)
MLLPGLLLLLLLLLLAGTRWLWGQWKLWKLRLPPLAPGFLHFLQPNLPVYLFGLAQKLGP
IYRIRLGLQDVVVLNSNKTIEEALIQKWVDFAGRPQILDGKMNFDLSMGDYSLTWKAHKK
LSRSALVLGMRDSMEPLVEQLTQEFCERMRAQAGASVAIHKEFSLLTCSIISCLTFGDKQ
DSTLLNATHSCVRDLLKAWNHWSVQILDIIPFLRFFPNPGLWKLKQFQESRDHIVMQELK
RHKDSLVAGQWKDMIDYMLQGVEKQRDARDPGQLHERHVHMSVVDLFVGGTETTAATLSW
AVAFLLHHPEIQKRLQEELDLKLAPSSQLLYKNRMQLPLLMATIAEVLRLRPVVPMALPH
RATKASSISGYDIPKDTIIIPNIQGANLDEMVWELPSKFWPDRFLESGKSPRIPTFGCGA
RVCLGEPLARLEFFVVLARLLQTFTLLPPPDGTLPSLQPLPYTGINLLIPPFQVRLQPRN
LAPQDQGQK
CYP21 rat
GenEMBL U56854
alternatively spliced form
Cyp21 mouse
genEMBL AF049850 Mm.18845
MLLPGLLLLLLLLAGTRWLWGQWKLRKLHLPPLAPGFLHFLQPN
LPIYLLGLTQKLGPIYRIRLGMQ DVVVLNSNRTIEEALIQKWVDFAGRPHMLN GKMDL
DLSLGDYSLMWKAHKKLSRSALMLGMRDSMEPLIEQLTQEFCERMRAQAGTPVAIHKE
FSFLTCSIISCLTFGDKDSTLVQTLHDCVQDLLQAWNHWSIQILTIIPLLRFLPNPGL
QKLKQIQESRDHIVKQQLKRHKDSLVAGQWKDMIDYMLQGVEKQRDGKDEERLHEGHV
HMSVVDLFIGGTETTATTLSWAVAFLLHHPEIQKRLQEELDLKLGPGSQLLYRNRMQL
PLLMATIAEVLRLRPVVPLALPHRATRASSISGYDIPKDMVIIPNIQGANLDEMVWEL
PSKFWPDRFLEPGKNPRTPSFGCGARVCLGEPLARLELFVVLARLLQAFTLLPPPDGT
LPSLQPQPYAGINLPIPPFQVRLQPRNLAPQDQGERP
Cyp21a2-ps mouse
GenEMBL AF030001
MLLPGLLLLLLLLAGTRWLWGQWKFRKLHLLPLAPGFLHFLQPNLPIYLLGLTQKLGPIYRIRLGLQ
Gap 30 aa missing
GKMDLDLSLGDYSLMWKAHKKLSRSALMLGMLDSMEPLIEQLTQEFCE
RMRAQAGTPVAIHKEFSFLTCSIISCLTFGDK
DSTLVQTLHDCVQDLLQAWNHWSIQILTIIPLLR
FFPNPGLQKLKQIQESRDHIIKQQLKQHK
DSLVAGQWKDMIDYMLQEVEKQRDGKDKEQLHEGHVHMSVVDLFIGGTETTATTLSWAVAFLLHHPE
IQK*LQEELDLKLGPGSQLLYRNRMQLPLLMATIAEVLHLRPVVPLALPHRATKAS
SISGYNIPKDMVIIPNIQGANLDEMVWELPSKFWP
DRFLEPGKNPRTPSFGCGARVCLGEPLARLELFVVLARLLQAFTLLPPPDGTLPSLQPQL
YAGINLLIPPFQVRLQPRNLAAQELGEKTLTG*
CYP21 pig
GenEMBL S53049 M83939 (4792bp) Swiss Q02390 (492 amino acids)
PIR S28169 (492 amino acids)
Burghelle-Mayeur,C., Geffrotin,C. and Vaiman,M.
Sequences of the swine 21-hydroxylase gene (Cyp21) and
a portion of the opposite-strand overlapping gene of unkown
function previously described in human.
Biochim. Biophys. Acta 1171, 153-161 (1992)
CYP21 sheep
PIR A43349 (497 amino acids) B43349 (479 amino acids)
Crawford, R.J., Hammond, V.E., Connell, J.M. and Coghlan, J.P.
The structure and activity of two cytochrome P450c21 proteins
encoded in the ovine adrenal cortex.
J. Biol. Chem. 267, 16212-16218 (1992)
B43349 is truncated early after alternate splicing
CYP21 Xenopus tropicalis
See Xenopus pages for seq
CYP21A1 Danio rerio (zebrafish)
See xebrafish pages for seq
CYP21 Fugu rubripes (pufferfish)
No accession number
Scaffold_15
44% to CYP21 human over 454 amino acids
this is the first fish CYP21 sequence found
MCSFLFCSSFSAPPAVKRNLPLSLWQLPLRPSSPPIPGPPCRFLIGNMTE
28230 LMHDHLPIHLTNLAKRYGNIYRLKCGNTT 28144 (1)
28058 AMIVLNSSDIIREALVKKWSDFAGRAVSYT 27969 (1)
27888 ADIVSGGGRNISLGDYTEEWKALRRLVHGALQRCCKHSLHNVIERQALQLRK 27754 (0)
VLVDYRGGAVDLSEDFTVAASNVIITLVFGKE (0)
YDKSSSELQQLHRCLNEIVALWGSTWISALDTFPLLR (0)
KFPNPVFSRLLREVSRRDEIIRKHLNQFK (0)
CVLCCAQSEGHRRTDVITGSLLEG (0)
26896 VLTDMHVHMATVDLLIGGSETTAAWLNWTVAFLLHRPE (0)
FQTKVYEELCTVLEGRYPKYSDRQRLPILCSLIHEVLRLRPVAPLAVPHKAIRDS (2)
SIAGYFIPRNTIIIPNLFGAHHDPEVWSDPYSFKP (1)
26238 ERFLEGGGGSTRALIPFGGGARLCLGETVAKMELFLFTAYLLRDFCFVLPDSEAPLPDLR 26059
26058 GVASVVLKIKSFTVIARPRTGP* 25990
CYP21 Tetraodon nigroviridis (freshwater pufferfish)
GenEMBL AL281449.1 C0BG094DE12LP1 G Tetraodon nigroviridis
genomic clone 094J24 T7.Length = 895
AL233853.1 C0BG007BE04XD1 G Tetraodon nigroviridis
genomic clone 007I08 T7.Length = 1079
86% to Fugu CYP21
MGCIFFFFYLPFSAPPAVKRSLLQSLCGLLHRPSSPSIPGPPCRFLIGNMTE
LMQDHLPIHLTDLAKRYGNIYRLKCGNTS
AMVVLSSGDVIREALVKKWSDFAGRSVSYT
ADIVSGGGRTISLGDYTEEWKAHRRLVHSAL (frameshift) ERCXKQSLHDVIERQALQLRK
Missing exon 4 and part of exon 5
GSAWISALDTFPLLR
KFPNPVFSRLLREVTRRDEIIRKHLNQYK
CVLCCVQSQDNKSTDVITGSLLEG
VLTDVHVHMATVDLLIGGTETTAAWLNWTVAFLLHRPE
IQTKVYEELCTVLEGRYPKYSDRHRLPVLCSLVHEVLRLRPVAPLAVPHKAVRDS
SIAGYFIPKNTIIIPNLFGAHHDPXVWPDPYSFXX
Missing exon 11
22A Subfamily
CYP22A1 Caenorhabditis elegans (nematode worm)
GenEMBL U39648 (28581 bp) AF407572, NM_171699, NM_171698
cosmid T13C5.1
Jia,K., Albert,P.S. and Riddle,D.L.
DAF-9, a cytochrome P450 regulating C. elegans larval development
and adult longevity
Development 129 (1), 221-231 (2002)
daf-9 mutant This gene may be in the pathway to
synthesize a ligand for DAF-12, a nuclear receptor.
Daf-9 has a role in larval development.
DAF comes from abnormal DAuer Formation
23A Subfamily
CYP23A1 Caenorhabditis elegans (nematode worm)
GenEMBL U39472 (42282 bp)
see CYP14A1 for reference
cosmid B0304.3
24A Subfamily
CYP24 rat
GenEMBL L04608 to L04619, S52625 to S52636
Ohyama,Y., Noshiro,M., Eggertsen,G., Gotoh,O., Kato,Y.,
Bjorkhem,I. and Okuda,K.
Structural characterization of the gene encoding rat
25-hydroxyvitamin D3 24-hydroxylase.
Biochemistry 32, 76-82 (1993)
CYP24 rat
GenEMBL Z28351 (1419bp)
Hahn,C.N., Kerry,D.M., Omdahl,J.L. and May,B.K.
Identification of a vitamin D responsive element in the promoter of
the
gene for the rat 25-hydroxyvitamin D3 24-hydroxylase
Nuc. Acids Res. 22, 2410-2416 (1994)
CYP24 rat
GenEMBL X59506 (3209bp)
Ohyama,Y., Noshiro,M. and Okuda,K.
Cloning and expression of cDNA encoding 25-hydroxyvitamin D3 24-
hydroxylase.
FEBS Lett. 278, 195-198 (1991)
CYP24 rat
GenEMBL D17792 (?bp)
Ohyama, Y., Ozono,K., Uchida,M., Shinki,T., Kato,S., Suda,T.,
Yamamoto,O.,
Noshiro,M. and Kato,Y.
Identification of a vitamin D-responsive element in the 5'-flanking
region of the rat
25-hydroxyvitamin D3 24-hydroxylase gene.
J. Biol. Chem. 269, 10545-10550 (1994)
CYP24 rat
GenEMBL U03112 (?bp)
Zierold,C., Darwish,H.M. and DeLuca,H.F.
Identification of a vitamin D-response element in the rat calcidiol
(25-hydroxyvitamin D3) 24-hydroxylase gene.
Proc. Natl. Acad.Sci. USA 91, 900-902 (1994)
CYP24 human
GenEMBL S67623 (776bp)
Labuda,M., Lemieux,N., Tihy,F., Prinster,C. and Glorieux,F.H.
Human 25-hydroxyvitamin D 24-hydroxylase cytochrome P450
subunit maps to a different chromosomal location than that of
pseudovitamin D-deficient rickets.
J. Bone Miner. Res. 8, 1397-1406 (1993)
CYP24 human
GenEMBL L13286 (3254bp)
Chen,K.-S., Prahl,J.M. and DeLuca,H.F.
Isolation and expression of 1,25-dihydroxyvitamin D3
24-hydroxylase cDNA.
Proc. Natl. Acad. Sci. USA 90, 4543-4547 (1993)
CYP24 human
NM_000782
MSSPISKSRSLAAFLQQLRSPRQPPRLVTSTAYTSPQPREVPVC
PLTAGGETQNAAALPGPTSWPLLASLLQILWKGGLKKQHDTLVEYHKKYGKIFRMKLG
SFESVHLGSPCLLEALYRTESVPQRLEIKPWKAYRDYRKEGYGLLILEGEDWQRVRSA
FQKKLMKPGEVMKLDNKINEVLADFMGRIDELCDERGHVEDLYSELNKWSFESICLVL
YEKRFGLLQKNAGDEAVNFIMAIKTMMSTFGRMMVTPVELHKSLNTKVWQGHTLAWDT
IFKSVKACIDNRLEKYSQQPSADFLCDIYHQNRLSKKELYAAVTELQLAAVETTANSL
MWILYNLSRNPQVQQKLLKEIQSVLPENQRPREEDLRNMPYLKACLKESMRLTPGVPF
TTRTLDKATVLGEYALPKGTVLMLNTQVLGSSEDNFEDSSQFRPERWLQEKEKINPFA
HLPFGVGKRMCIGRRLAELQLHLALCWIVRKYDIQATDNEPVEMLHSGTLVPSRELPI
AFCQR
CYP24A1 Bos taurus (cow)
See cattle page for details
6035 MSSPISKSRSLAAFLQQLRSLGQPPRPVTSTACSPRRPREVPLCPVMEPGEAQDAATLP 6211
6212 GPTKWPLLGSLLEILWKGGLKKQHDTL (0)
6511 VEYHKKYGKIFRMKLGSFDSVHLGSPCLLEALYRTESAHPQRLEIKPWKAYRDYREEGYGLLIL(2) 6702
7484 EGEDWQRVRSAFPKKLMKPVEIMKLDDKINE(0) 7573
4559 VLADFMGRIDELCDERGRIEDLYTELNKWSFE (1)
2100 SICLVLYEKRFGLLQKNAGEEALNFIMAVKT (0)
1035 MMSMFGKMMVTPVELHRSLNTRVWQAHTQAWDTIFRS (1) 925
1303 VKSCVDNRLEKYSEQPSMDFLCDIYHHNQLSKKELYAAVTELQLAAVET (0) 1449
TANSLMWILYNLSRNPHVQQKLFKEIQSVLPENQLPRAEDLRNMPYLKACLKESMR
LNPTVPFTTRTLDKAMVLGEYALPKG
5393 TVLVLNTHVLGSSEENFEESSQFRPERWLQDKKNLNPFAHLPFGVGKRMCVGRRLAELQL 5572
5573 HLALCW (0) 5602
IVRKYDVVATDLEPVETLHLGNLVPGRQLPVAFCQR
CYP24A1 pig
NM_214075
MSSPISKSGSLAALLQQLRSCGQPPRPVTSTACVPRRSKEVPLC
PMEQPGETQDAAXAGSTRWPLLGSLLEILWKGGLKKQHDTLAEYHRKYGKIFRMKLGS
FDSVHLGSPCLLEALYRTESAHPQRLEIKPWKAYRDYRKEGYGLLILEGEDWQRVRSA
FQKKLMKPMEVMKLDNKINEVLADFMSRIDELCDERGCIEDLYSELNKWSFESICLVL
YEKRFGLLQKNAGEEALNFITAIKTMMSTFGKMMVTPVELHKNLNTKVWQAHTLAWDT
IFKSVKSCIDHRLEKYAEQPSADFLCDIYHHNQLSKKELYASVTELQLAAIETTANSL
MWILYNLSRNPHVQQKLLKEIQSVLPENQMPRAEDLRNMPYLKACLKESMRLTPSVPF
TTRTLDKAMVLGEYALPKGTVLMLNTYVLGTNEENFEDSSQFRPERWLQEKKKINPFA
HLPFGVGKRMCIGRRLAELQLHLALCRIIRKYAIVATDSEPVGMLHLGILDPTRQLPI
AFCQR
Cyp24 mouse
GenEMBL D89669, AC084066.1, Mm.6575
MSCPIDKRRPLIAFLRRLRDLGQPPRSVTSKAHVKRAPKEVPLC
PLMTDGETRNVTSLPGPTNWPLLGSLLEIFWKGGLKKQHDTLAEYHKKYGQIFRMKLG
SFDSVHLGSPSLLEALYRTESAHPQRLEIKPWKAYRDHRNEAYGLMILEGQEWQRVRS
AFQKKLMKPVEIMKLDKKINEVLADFMGQIDELRDERGRIQDLYSELNKWSFESICLV
LYEKRFGLLQKDTEEEALTFIAAIKTMMSTFGKMMVTPVELHKRLNTKVWQAHTLAWD
TIFKSVKPCIDHRLERYSQQPGADFLCDIYQQDHLSKKELYAAVTELQLAAVETTANS
LMWILYNLSRNPQVQQRLLREIQSVLPDNQTPRAEDVRNMPYLKACLKESMRLTPSVP
FTTRTLDKPTVLGEYTLPKGTVLTLNTQVLGSSEDNFEDADKFRPERWLEKEKKINPF
AHLPFGVGKRMCIGRRLAELQLHLALCWIIQKYNIVATDSEPVEMLHLGILVPSRELP
IAFCPR
CYP24A1 Xenopus tropicalis
See Xenopus pages for seq
CYP24A1 Danio rerio (zebrafish)
See xebrafish pages for seq
CYP24 Fugu rubripes (pufferfish)
No accession number
Scaffold_4128
MLWRLRGALTLPPELTVLDAIPGPTNWPLVGSLFELLRKGGLTRQHEAL
VDYHKKFGKIFRLKLGSFESVHIGAPCLLESLYRTEGSYPQRLEIKPWTAYRDMRDEAYGLLIL
EGKDWQRVRRAFQQKLMKPTEVVKLDRKINE
VLEDFVSRIGKTNIGGKIEDLYFELNKWSFES
ICLVLYDKRFGLLQDKVNEEAMNFITAVKT
MMSTFGLMMVTPVELHKSLNTKTWQDHTAAWDRIFST
AKVYIDKKLKRNSVIAPDDLIGDILHQSRLSKKELYAAITELQIGGVET
8608 TANSMLWAIFNLSRNPGAQRRLLEEIRTVVPPEQDPCGEHIKSMPYLKACLKESMR 8441 (2)
7830 ISPSVPFTSRTLDKDTVLGDYAIPKG 7753
TVLMINSHALGSSEDYFDDGKKFKPERWLREHGTINPFAH
VPFGIGKRMCIGRRLAELQMSLFLQLVRDFE
IVATDNEPLDVIHSGLLVPNRELPVAFIKR
25A Subfamily
CYP25A1 Caenorhabditis elegans (nematode worm)
GenEMBL Z66495 ( 40145bp)
see CYP14A1 for reference
cosmid C36A4.1
CYP25A2 Caenorhabditis elegans (nematode worm)
GenEMBL Z66495 ( 40145bp)
see CYP14A1 for reference
cosmid C36A4.2
CYP25A3 Caenorhabditis elegans (nematode worm)
GenEMBL Z66495 ( 40145bp)
see CYP14A1 for reference
cosmid C36A4.3
CYP25A4 Caenorhabditis elegans (nematode worm)
GenEMBL Z66495 ( 40145bp)
see CYP14A1 for reference
cosmid C36A4.6
CYP25A5 Caenorhabditis elegans (nematode worm)
GenEMBL AF038613
see CYP14A1 for reference
F42A6
CYP25A6P Caenorhabditis elegans (nematode worm)
GenEMBL U50072
K06B9.1
missing C-terminal
26A Subfamily
Cyp26a1 mouse
No accession number
Jim Ray
submitted to nomenclature committee
Note: new family in mammals, homolog to human ESTs R51129 and R21282
Cyp26a1 mouse
No accession number
Martin Petkovich
submitted to nomenclature committee
Note: new family in mammals, homolog to human ESTs R51129 and R21282
Cyp26a1 mouse
GenEMBL Y12657
Fujii, H., Sato, T., Kaneko, S., Gotoh, O., Fujii-Kiriyama, Y.,
Osawa, K.,
Kato, S. and Hamada, H.
Metabolic inactivation of retinoic acid by a novel P450
differentially expressed in
developing mouse embryos.
EMBO J. 16, 4163-4173 (1997)
Note: new family in mammals, homolog to human ESTs R51129 and R21282
Cyp26a1 mouse
GenEMBL Y12657 NM_007811 EST AA239785
no UNIGENE entry
MGLPALLASALCTFVLPLLLFLAALKLWDLYCVSSRDRSCALPL
PPGTMGFPFFGETLQMVLQRRKFLQMKRRKYGFIYKTHLFGRPTVRVMGADNVRRILL
GEHRLVSVHWPASVRTILGAGCLSNLHDSSHKQRKKVIMQAFSREALQCYVLVIAEEV
SSCLEQWLSCGERGLLVYPEVKRLMFRIAMRILLGCEPGPAGGGEDEQQLVEAFEEMT
RNLFSLPIDVPFSGLYRGVKARNLIHARIEENIRAKIRRLQATEPDGGCKDALQLLIE
HSWERGERLDMQALKQSSTELLFGGHETTASAATSLITYLGLYPHVLQKVREEIKSKG
LLCKSNQDNKLDMETLEQLKYIGCVIKETLRLNPPVPGGFRVALKTFELNGYQIPKGW
NVIYSICDTHDVADIFTNKEEFNPDRFIVPHPEDASRFSFIPFGGGLRSCVGKEFAKI
LLKIFTVELARHCDWQLLNGPPTMKTSPTVYPVDNLPARFTYFQGDI
CYP26A1 human
GenEMBL NM_000783
White,J.A., Beckett-Jones,B., Guo,Y.D., Dilworth,F.J., Bonasoro,J.,
Jones,G. and Petkovich,M.
cDNA cloning of human retinoic acid-metabolizing enzyme (hP450RAI)
identifies a novel family of cytochromes P450
J. Biol. Chem. 272 (30), 18538-18541 (1997)
Note: new family in mammals, equal to human ESTs R51129 and R21282
CYP26A1 human
NM_000783
MGLPALLASALCTFVLPLLLFLAAIKLWDLYCVSGRDRSCALPL
PPGTMGFPFFGETLQMVLQRRKFLQMKRRKYGFIYKTHLFGRPTVRVMGADNVRRILL
GDDRLVSVHWPASVRTILGSGCLSNLHDSSHKQRKKVIMRAFSREALECYVPVITEEV
GSSLEQWLSCGERGLLVYPEVKRLMFRIAMRILLGCEPQLAGDGDSEQQLVEAFEEMT
RNLFSLPIDVPFSGLYRGMKARNLIHARIEQNIRAKICGLRASEAGQGCKDALQLLIE
HSWERGERLDMQALKQSSTELLFGGHETTASAATSLITYLGLYPHVLQKVREELKSKG
LLCKSNQDNKLDMEILEQLKYIGCVIKETLRLNPPVPGGFRVALKTFELNGYQIPKGW
NVIYSICDTHDVAEIFTNKEEFNPDRFMLPHPEDASRFSFIPFGGGLRSCVGKEFAKI
LLKIFTVELARHCDWQLLNGPPTMKTSPTVYPVDNLPARFTHFHGEI
CYP26A1 Bos taurus (cow)
See cattle page for details
MGLPALLASALCTFVLPLLLFLAAIKLWDLYCVSSRDRSCALPL
PPGTMGFPFFGETLQMVLQREKFLQMKRRKYGFIYKTHLFGRPTVRVMGADNVRRILL
GEHRLVSVHWPASVRTILGSGCLSNLHDSSHKQRKK (0)
VIMQAFSREALQCYVPVIAEEVGNYLEQWLSCGERGLLVYPQVKRLMFRIAMRILLGCE
SRLASGGEDEQQLVEAFEEMTRNLFSLPIDVPFSGLYR
GLKARDLIHARIEENIRAKIRRLPAAEAGGGCKDALQLLIEHSWERGERLDMQ
ALKQSSTELLFGGHETTASAATSLITYLGLYPHVLQKVREELKNK
GLLCKGNQDNKLDVEI
LEQLKYIGCVIKETLRLNPPVPGGFRVALKTFELNGYQIPKGWNVIYSICDTHDVADIFT 182
183 NKEEFNPDRFLLPHPEDASRFSFIPFGG
GLRSCVGKEFAKILLKIFTVELARHCDWQLLNGPPTMKTSPTVYPVDDLPARFTRFQGEI* 449
CYP26A1 Sus scrofa (miniature pig)
no accession number
Haitao Shang
Submitted to nomenclature committee May 23, 2007
partial sequence
92% to human 26A1, full seq. constructed from ESTs
Ortholog of human CYP26A1
CYP26A1 Sus scrofa (pig)
Pig ESTs CN161524.1, CN162920.1, CF179212.1, DY434075.1,
CF180567.1, CN165481.1,
93% to human 26A1
MGLPALLASVLCTFVLPLLLFLAAIKLWDLYCVSSRDRSCTLPLPPGTM
GFPFFGETLQMVLQRRKFLQMKRRKYGFIYKTHLFGRPTVRVMGADNVRRILLGEHRLVS
VHWPASVRTILGSGCLSNL
HDSSHKQRKKVITQAFSREALQCYVPVIAEEVDSCLEQWLSCGERGLLVYPQVKR LMFRI
AMRILLGCEPRLASGGEAEQQLVEAFEEMTRNLFSLPIDVPSSGLYRGLKARNLIHARIE
ENIRAKICGLRAAGAGGGCKDALQLLIEHSWERGERLDMQALKQSSTELLFGGHETTASA
ATSLITYLGLYPHVLQKVREELKSKGLLCKGNQDNKLDMEILEQLKYIGCVIKETLRLNP
PVPGGFRVALKTFELNGYQIPKGWNVIYSICDTHDAADIFTNKEEFNPDRFLLPHPEDAS
RFSFIPFGGGLRSCVGKEFAKILLKIFTVELARHCDWRLLNGPPTMKTSPTVYPVDDLPA
RFTRFQGEI*
CYP26A1 zebra fish
GenEMBL U68234
White, J.A., Guo, Y.-D., Baetz, K., Beckett-Jones, B., Bonasoro, J.
Hsu, K.E., Dilworth, F.E., Jones, G. and Petkovich, M.
Identification of the retinoic acid-inducible all trans retinoic
acid 4-hydroxylase.
J. Biol. Chem. 271, 29922-29927 (1996)
Note: new family in vertebrates, homolog to human ESTs R51129 and
R21282
CYP26A1 Xenopus tropicalis
See Xenopus pages for seq
CYP26A1 Fugu rubripes (pufferfish)
No accession number
Scaffold_12575
67% to 26A1 human
missing C-term zebrafish seq shown in lower case
2144 MAVSALLATFLCTIVLPLLLFLVTVKLWEVY (frameshift)
VIRERDSACP (frameshift)
SPLPPG (frameshift)
TMGLPFIGETLQLILQ (0)
1689 RRKFLRMKRQKYGYIYRTHLFGNPTVRVTGANNVRHILLGEHRLVAV 1552
1551 QWPASVRTILGSDTLSNVHGAQHKTKKK 1465 (0)
1230 AIMQAFSREALEFYIPAMQHEVQAAVQEWLAKDSCVLVY
PEMKRLMFRIAMQILLGFQLE 1051 (frameshift)
1049 QIKTDEQKLVEAFEEMIKNLFSLPIDMPFSGLYR 948 (0)
784 GLKARNFIHAKIEENIKRKLRESNSDSKCRDALQQLIDSSKKSGQVLSMQ 635 (0)
548 VLKESATELLFGGHETTASTATSLIMFLGLNPEVLDKLRHELSDKVMHKGF 396 (1)
329 LDLRSLNLETLEQLKYTSCVIKETLRMNPPVPGGFRVALKTFELG 195 (0)
100 GYQIPKGWNVIYSICDTHDVAEIFP 26 (frameshift)
24 NKEDFQPE 1 end of Scaffold_12575
RFMMKNCGDSSRFQYIPFGGG
srmcvgkefakvllkiflveltqhcnwilsngpptmktgptiypvdnlptkftsyvrn
CYP26A1 Xenopus laevis
GenEMBL AF057566
Hollemann,T., Chen,Y., Grunz,H. and Pieler,T.
Regionalized metabolic activity establishes boundaries of retinoic
acid signalling.
EMBO J. 17, 7361-7372 (1998)
CYP26B1 human
GenEMBL AC007002
Nelson, D.R. A second CYP26 P450 in humans and zebrafish: CYP26B1
Archives of Biochemistry and Biophysics 371, 345-347 (1999)
MLFEGLDLVSALATLAACLVSVTLLLAVSQQLWQLRWAATRDKSCKLPIPKGSMGFPLIGETGHWLLQ
GSGFQSSRREKYGNVFKTHLLGRPLIRVTGAENVRKILMGEHHLVSTEWPRSTRMLLGPNTVSNS
IGDIHRNKRKVFSKIFSHEALESYLPKIQLVIQDTLRAWSSHPEAINVYQEAQ
KLTFRMAIRVLLGFSIPEEDLGHLFEVYQQFVDNVFSLPVDLPFSGYRR
GIQARQILQKGLEKAIREKLQCTQGKDYLDALDLLIESSKEHGKEMTMQELKDGTLELIF
AAYATTASASTSLIMQLLKHPTVLEKLRDELRAHGILHSGGCPCEGTLRLDTLSGLRYLD
CVIKEVMRLFTPISGGYRTVLQTFELDGFQIPKGWSVMYSIRDTHDTAPVFKDVNVFDP
DRFSQARSEDKDGRFHYLPFGGGVRTCLGKHLAKLFLKVLAVELASTSRFELATRTFPRI
TLVPVLHPVDGLSVKFFGLDSNQNEILPETEAMLSATV
CYP26B1 Bos taurus (cow)
See cattle page for details
MLFEGLELVSALATLAACLVSVTLLLAVSQQLWQLRWAATRDKSCKLPIPKGSMGFPLIGETGHWLLQ
GSGFQSSRREKYGNVFKTHLLGRPLIRVTGAENVRKILMGEHHLVSTEWPRSTRML
LGPNTVSNSIGDIHRNKR
VFSKIFSHEALESYMPKIQLVIQDTLRAWSSHPEAINVYQEAQKLTFRMAIRVLLG
FSLPVDLPFSGYRRGIQARQTLQKG
77 LEKAIREKLQCTQGKDYSDALDIFIESSKEHGKEMTMQELKDGTLELIFAAYAT 238
239 TASASTSLIMQLLKHPAVLEKLREELRAKGLLHSGGCPCEGTLRLDTLSGLHYLDCVIKE 418
419 VMRLFTPVSGGYRTVLQTFELDGFQIPKGWSVMYSIRDTHDTAPVFKDVNVFDPDRFG 592
593 QARSEDKDGRFHYLPFGGGVRTCLGKHLAKLFLKVL 700
AVELASTSRFELATRTFPRITLVPVLHPVDGLSVKFFGLDSNQNKILPETEAMLSATV*
CYP26B1 pig
AK238570.1, CJ034657.1
aa 88-142
LLGRPLIRVTGAENVRKILMGEHHLVSTEWPRSTRMLLGPNTVANSIGDIHRNKR
Cyp26b1 mouse
GenEMBL AC022779.3 AW047279 GSS AZ741670, AZ369731, BM936625.1
MLFEGLELVSALATLAACLVSVTLLLAVSQQLWQLRWAATRDKSCKLPIPKGSMGFPLIGETGHWLLQ (0)
GSGFQSSRREKYGNVFKTHLLGRPLIRVTGAENVRKILLGEHQLVSTEWPRSARVLLGPNTVANSIGDIHRNKRK (0)
VFSKIFSHEALESYLPKIQLVIQDTLRAWSSQPEAINVYQEAQRLTFRMAVRVLLGFSI
PEEDLGHLFEVYQQFVENVFSLPVDLPFSGYRR (?)
GIQARQILQKGLEKAIREKLQCTQGKDYSDALDILIESSKEHGKEMTMQELK
DGTLELIFAAYATTASASTSLIMQLLK
HPAVLEKLREELRAQGLLHGGGCPCEGTLRLDTLSSLRYLDCVIKEVMRLFTPVSGGYRTVLQTFELD (?)
GFQIPKGWSVMYSIRDTHDTAPVFKDVNVFDPDRFSQARSEDKDGRFHYLP
FGGGVRTCLGKHLAKLFLKVLAVELASTSRFELATRTFPRITLVPVLHPVDGLSVKFFGLDSNQNEILPETEAMLSATV
CYP26B1 Xenopus tropicalis
See Xenopus pages for seq
CYP26B1 Danio rerio (zebrafish)
See xebrafish pages for seq
CYP26B1 Fugu rubripes (pufferfish)
No accession number
Scaffold_4267
74% to 26B1 human
8448 MLFDSFDLVSALATLAACLVSMALLLAVSQQLWQLRWTATRDRNCKLPMPKGSMGFPFIGETCHWLLQ 8651
17930 GSGFHASRRQKYGNVFKTHLLGRPLIRVTGAENIRKVLMGEHTLVTVDWPQSTSTLLGPNSLA 18118
18119 NSIGDIHRKKRK 18154
1487 VFAKVFSHEALESYLPKIQQVIQESLRVWSSNPEPINVYR 1606 (2)
1689 ESQRLSFTMAVRVLLGFRVSEEEMKHLFSTFQDFVDNLFSLPIDLPFSGYRK 1844 (0)
1921 GIRARDTLQKSIEKAIREKPLCSQGKDYSDALDVLMESAKENGSELTMQELK 2076 (0) exon 4
2851 ESTIELIFAAFATTASASTSLIMQLLRHPPVLERLREELRARGL exon 5,6 fused
LHNGCLCPEGELRLDTIVSLKYLDCVIKEVLRLFTPVSGAYRTAMQTFELD 3135 (0) exon 5,6 fused
3271 GVQIPKGWSVMYSIRDTHDTSTVFKDVDVFDPDRFSQERGEDKEGRFHYLPFGGGVRSCLGKQLA exon 7
TLFLRILAIELASTSRFELATRQFPRVITVPVVHPVDGLKVKFYGLDSNQNEIMAKSEELLGAAV* 3663 exon 7
CYP26C1 human
GenEMBL AL358613.11 May 2, 2001
522 amino acids, 6 exons, (0) = phase 0 intron
52% to 26B1 human, also 15 amino acid insertion in exon 5 vs. 26B1
MFPWGLSCLSVLGAAGTALLCAGLLLSLAQHLWTLRWMLSRDRASTLPLPKGSMGWPFFGETLHWLVQ (0)
GSRFHSSRRERYGTVFKTHLLGRPVIRVSGAENVRTILLGEHRLVRSQWPQSAHILLGSHTLLGAVGEPHRRRRK (0)
VLARVFSRAALERYVPRLQGALRHEVRSWCAAGGPVSVYDASKALTFRMAARILLGLRL
DEAQCATLARTFEQLVENLFSLPLDVPFSGLRK (0)
GIRARDQLHRHLEGAISEKLHEDKAAEPGDALDLIIHSARELGHEPSMQELK (0)
ESAVELLFAAFFTTASASTSLVLLLLQHPAAIAKIREELVAQGLGRACGCAPGAAGGSEGPPPD
CGCEPDLSLAALGRLRYVDCVVKEVLRLLPPVSGGYRTALRTFELD (0)
GYQIPKGWSVMYSIRDTHETAAVYRSPPEGFDPERFGAAREDSRGASSRLHYIPFGGGARSCLG
QELAQAVLQLLAVELVRTARWELATPAFPAMQTVPIVHPVDGLRLFFHPLTPSVAGNGLCL*
CYP26C1 Bos taurus (cow)
See cattle page for details
67 MLPWGLSCLSALGAVGTALLGAGLLLSLAQHLWTLRWTLSRDRASALPLPKGSMGWPFFGETLHWLVQ (0)
GSRFHSSRRERYGTVFKTHLLGRPVIRVSGAENVRTVLLGEHRLVRSQWPQSAHILLGSHTLLGAVGESHRQRRK (0)
5955 ILARAFSRAALECYVPRLQRALRREVRSWCAARGPVAVYEAAKALTFRMAARILLGLR 6134
6135 LDEAQCSELARTFEQFVENLFSLPLDVPFSGLRK (0) 6251
GIRARDQLHRHLEEAIAQKLLEDKTAVEPGDALDGIIHSTRELGHELSVQELK (0)
46 ESAVELLFAAFSTTASASTSLVLLLLQHPAAIAKIQQELAAQGLGRACGCAPAASGGG 225
226 AGPPPDCGCEPDLSLAALGHLRYVGCVVKEVLRLLPPVSGGYRTALRTFELD (0) 381
GYQIPKGWNVMYSIRDTHETAAVYRSPPEGF
DPERFGTAGDDALGAAGRFHYIPFGGGARSCLGQELAQTVLQLLAVELVRTARWELATPA 2322
2321 FPAMQTVPIVHPVDGLRLFFHPLASPAAQDGQRS*
CYP26C1 mouse
GenEMBL AC110212.1
84% to 26C1 human exon 5
ELAVELLFAAFFTTASASTSLILLLLQHPAAITKIQQELSAQGLGRACTCTPRASGSPPDCGCEPDLSLAMLGRLRYVDCVVKEVLRLLPPVSGGYRTALRTFELD
Cyp26c1 mouse
GenEMBL XM_140712.1 May 17, 2002 also AC110212.4
1 MISWGLSCLSVLGAAGTTLLCAGLLLGLAQQLWTLRWTLSRDWASTLPLPKGSMGWPFFG 180
181 ETLHWLVQGSRFHSSRRERYGTVFKTHLLGRPVIRVSGAENVRTILLGEHRL 338
339 VLARVFSRSSLEQFVPRLQGALRREVRSWCAAQRPVA 447
448 VYQAAKALTFRMAARILLGLQLDEARCTELAHTFEQLVENLFSLPLDVPFSGLRKGIRAR 627
628 DQLYEHLDEAVAEKLQEKQTAEPGDALLLIINSARELGHEPSVQELK
ELAVELL 987
988 FAAFFTTASASTSLILLLLQHPAAITKIQQELSAQGLGRACTCTPRASGSPPDCGC 1155
1156 EPDLSLAMLGRLRYVDCVVKEVLRLLPPVSGGYRTALRTFELDGYQIPKGWSVMYSIRDT 1335
1336 HETAAVYRSPPEGFDPERFGVESGDARGSGGRFHYIPFGGGARSCLGQELAQAVLQLLAV 1515
1516 ELVRTARWELATPAFPVMQTVPIVHPVDGLLLFFHPLPTSGAGDGLPF*
CYP26C1 Xenopus tropicalis
See Xenopus pages for seq
CYP26C1 Danio rerio (zebrafish)
See xebrafish pages for seq
CYP26C1 Fugu rubripes (pufferfish)
No accession number
Scaffold_11741
Equally similar to 26B1 and 26C1 human
But C-terminal is 68% to 26C1 while 58% to 26B1
Lower case region very poor match may not be correct exon structure here.
6362 MLGLVSALATALTTLLLLLLLLALTRQLWSFRWSLTRDRRCELPLPKGSMGWPLVGETFQWLFQ 6171 (0)
5571 GSNFHISRRKRHGNVFKTHLLGKPLVRVTGAENIRKILLGEHSLVCTQWPQSTRIILGPN 5392
5391 ALVNSIGELHKRKRK 5347 (0)
4963 ILAKVFSRKALESYLPRLQEVIKCEIAKWCAEPGSVDVYAATRSLTFRIAIGVLLGLHL 4781
4780 EEERIDYLAQIFGQLMSNLFSLPIDAPFSGLRK (0)
3972 GIKARKILHANMEKIIEKKMERQQEEEEYRDAFDYMLSTSKEQGQQISIQELK 3814 (0)
3581 ETAVELIFAAHSTTASAATSLVLQLLHHPEVVERVRVELEAQKLcynslnlpsqa 3417 (1)
3377 ctfpqsqchasnLSLDKLNQLHYIDCVIKEVLRFLPPVSGGYRTALQTFELD 3222 (0)
2770 GYQIPKGWTVMYSIRDTHETAEIFQNPELFDPDRFVTAQVESRSSRFSYVPFGGGVR 2600
2599 SCVGKELAQIILKTLTIELIRTCKWTLATEKFPKMQTVPIVHPVNGLHVNFMYKNLHEIDH* 2414
27A Subfamily
CYP27A1 human
Swiss Q02318 (531 amino acids)
Cali J.J., Russell D.W.
Characterization of the human sterol-27-hydroxylase: A mitochondrial
P450
that catalyzes multiple oxidation reactions in bile acid
biosynthesis.
J. Biol. Chem. 266, 7774-7778 (1991)
CYP27A1 human
GenEMBL X59812 (2107bp)
Guo,Y., Strugnell,S., Back,D.W. and Jones,G.
Transfected human liver cytochrome P-450 hydroxylates vitamin D
analogs at different side-chain positions
Proc. Natl. Acad. Sci. U.S.A. 90, 8668-8672 (1993)
CYP27A1 human
NM_000784
MAALGCARLRWALRGAGRGLCPHGARAKAAIPAALPSDKATGAP
GAGPGVRRRQRSLEEIPRLGQLRFFFQLFVQGYALQLHQLQVLYKAKYGPMWMSYLGP
QMHVNLASAPLLEQVMRQEGKYPVRNDMELWKEHRDQHDLTYGPFTTEGHHWYQLRQA
LNQRLLKPAEAALYTDAFNEVIDDFMTRLDQLRAESASGNQVSDMAQLFYYFALEAIC
YILFEKRIGCLQRSIPEDTVTFVRSIGLMFQNSLYATFLPKWTRPVLPFWKRYLDGWN
AIFSFGKKLIDEKLEDMEAQLQAAGPDGIQVSGYLHFLLASGQLSPREAMGSLPELLM
AGVDTTSNTLTWALYHLSKDPEIQEALHEEVVGVVPAGQVPQHKDFAHMPLLKAVLKE
TLRLYPVVPTNSRIIEKEIEVDGFLFPKNTQFVFCHYVVSRDPTAFSEPESFQPHRWL
RNSQPATPRIQHPFGSVPFGYGVRACLGRRIAELEMQLLLARLIQKYKVVLAPETGEL
KSVARIVLVPNKKVGLQFLQRQC
CYP27A1 Macaca fasicularis (cynomolgus monkey)
No accession number
Yasuhiro Uno
Submitted to nomenclature committee 1/11/2005
Clone name mfCYP27A1_18D1
100% to AB125162, 97% to human
CYP27A1 Macaca fasicularis (cynomolgus monkey)
GenEMBL AB125162
Kusuda,J., Osada,N., Tanuma,R., Hirata,M., Sugano,S. and
Hashimoto,K.
Isolation and characterization of cDNA for macaque neurological
disease genes
Unpublished, partial seq
CYP27A1 Bos taurus (cow)
See cattle page for details
MNRMGALGSARLRWALLGRRAALPGLGSFGARAKAAIPSALPAAQAAEAPGTGPGDRRLRSLD
ELSGPGQLRLLFQLLVQGYVLHLHQLQVLNKAKYGPIWINRVGPQMHVHLASAPLLEQVM
RQEGKYPVRDDMKLWKEHRDQQGLSYGPFTTMGEQWYRLRQTLNQRMLKPAEAALYTDAL
NEVINDFMDQLKQLRAESASGDHVPDIAHQFYFFALEAISYILFEKRIGCLERSIPKDTE
TFVRSVGLMFHNSLFVTFLPTWTRPLLPFWKRYLDGWNTIFSFGKKLIDQKLEEIEAQLK
TENPEKTQISGYLHFLLTSG
QLSPREAEGSLPELLLAGVDTTSNTLTWALYHLSKNPEIQAALHKEVVGVVPAGQVPQHK 961
DLARMPLLKAVLKETLRLYPVVPVNSRVVVDKEIEVGGFLFPKNTQFVLCHYVISRDPDI 1141
YPEPDSFQPQRWLRKNQPDALKTQHPFGSVPFGYGVRACLGRRIAELEMQLLLTRLIQHYE 1324
VVLAPETGEVTSVARIVLVPNKKVGLRFLQRQS*
CYP27A1 Sus scrofa (miniature pig)
no accession number
Haitao Shang
Submitted to nomenclature committee May 23, 2007
partial sequence
3 amino acid differences to complete seq AK232936.1
Ortholog of human CYP27A1
CYP27A1 pig
AK232936.1
MAALGCARLRWALLGTRVPFPVIGSQGARAKAAIPTALPAAQTAEAPGTGPGDRRLRTLKELSGPGRLRLLFQLLAQGYVLHLHQLQVLNKAKYGPMWINYIGPQIHVNVASAPLLEQVMRQEGKYPVRNDMDLWKEHRDLQGLTYGPFTTEGESWYRLRQALNQRMLKPADAALYTNALNEVVDDFMVRLNQLRAESTLGDHVPDIAHQFYFFALEAICYILFEKHIGCLERSIPQDTKAFVRSVGFMFQNSLYATFLPKWTRPLLPYWRRYLDGWNTIFSFGKKLIDQKLEEIEAQLQAGGPEEPRISGYLHLLLTSGQLSPREAEGSLPELLMAGVDTTSNTLTWALYHLSKNPEIQAALHEEVVGMVPAGRVPQYKDLAHMPLLKAVLKETLRLYPVVPVNSRVTDKEIEVGGFLFPKNTQFVLCHYVVSRDPSIFPEPDRFSPKRWLRKNQTDALKGQHPFGSVPFGYGVRGCLGRRIAELEMQLLLTRLIQQYEVTLAPETGEVQSVARIVLVPNKKVGLRFLQRQG
CYP27A1 rat
GenEMBL M73231 (2300bp)
Shayiq,R.M. and Avadhani,N.G.
Sequence complementarity between the 5'-terminal regions of mRNAs
for
rat mitochondrial cytochrome P-450c27/25 and a growth hormone-
inducible
serine protease inhibitor: a possible gene overlap.
J. Biol. Chem. 267, 2421-2428 (1992)
CYP27A1 rat
GenEMBL U17363, U17369 to U17376 genomic sequence
Mullick, J., Addya,S., Sucharov,C. and Avadhani,N.G.
Localization of a transcription promoter within the second exon of
the cytochrome P-450c27/25 gene for the expression of the major
species of two-kilobase mRNA.
Biochemistry 34, 13729-13742 (1995)
Cyp27a1 mouse
GenEMBL 8 ESTs follow AI286988 AK004977.1 Mm.26793
MAAWSRTRLRWTLLDPRVVGRGLCPQGARAKATIPAALQAQESTEGPGTGQDRPRLRSP
AELPGTGTLQFLFQLFLQGYVLHLPDLQVLNKTKYGPMWTTSFGTYTNVNLASAPLLEQV
MRQEGKYPIRDHMDQWKDHRDHKGLTYGIFIAQGEQWYHLRQALKQRLLKPDEAALYTDA
LNEVISDFITRLDQVRAESESGDQVPDMAHLLYHLALEAITYILFEKRIGCLKPSIPEDT
AAFIRSVAIMFQNSVYITFLPKWTRPLLPFWKRYLNGWDNIFSFGKKLID EKVQELKAQL
QETGPDGVRVSGYLHFLLTNELLSTQETIGTFPELLLAGVDT TSNTLTWALYHLSKSPEI
QEALHKEVTGVVPFGKVPQHKDFAHMPLLKAVIKETLRLYPVVPTNSRIITEKETEINGF
LFPKNTQFVLCHYVVSRDPSVFPEPNSFQPHRWLRKKEADNPGILHPFGSVPFGYGVRSC
LGRRIAELEMQLMLSRLVQKYEIALAPGMGEVKTVSRIVLVPSKKVRLHFLQRQ
CYP27A1 rabbit
PIR A90152 (21 amino acids)
Dahlbaeck, H.
Characterization of the liver mitochondrial cytochrome P-450
catalyzing the 26-hydroxylation of 5beta-cholestane-3alpha,
7alpha,12alpha-triol.
Biochem. Biophys. Res. Commun. 157, 30-36 (1988)
PIR A90155 (21 amino acids)
Dahlbaeck, H.
Biochem. Biophys. Res. Commun. (1989) 159:370
CYP27A1 pig
no accession number
Kjell Wikvall
77% identical to human
CYP27A1X Xenopus tropicalis
See Xenopus CYP27A8, CYP27A9 and CYP27A10
CYP27A1 Fugu rubripes (pufferfish)
No accession number
Scaffold_3437
46% to 27A1
MLLPIVLIPQHFCAFPLHVCLPLCKL
MWKKGSWLLRPLAWARSCSGGAGRVSTVPASPPNLRFKPRTVEDLPRVSLWELLYRLTFQGFHSHMHELQ (0)
136 ILEKGRYGPIYRNGMNAVSVSTAKLLGEVLRNDDKFPNRGDMSIWKEYRDLRGYGYGPFTE 321 (2)
536 KDERWYNLRAVLNKRMLRPKDALQYGDTIGEVVTDFIRRIYFLRQRSPTGDVVTDLNNELYHFSLE 733 (1)
816 AIASILFETRLGCLEEEIPTGTQDFINAISQMFSNNFQVFLMPKWSRGVLPYWRRYVAGWDGIFSF 1013 (1)
1206 ATRLIDRKMEFIQQHLDNNQNVEGEYLTYLLSNTQMSIKDVYGSVSELLLAGVDT 1370 (0)
1465 TSNTLTWTLHLLSKYPQCQEILFKEVSTSVPADRAPSAEEVTRMPYLRAVVKESLR 1632 (2)
1756 MFPVIPMNGRILADKDVMIGGYQFSKN 1836 (0)
1949 TAFNFSHYAIGRDEDTFPEPATFMPERWLQDSHNRPNAFGAIAFGFGVRGCVGRRIAELEMYSFLCH 2149 (0)
2308 LMRHFEIKPDPKMGELKSVCRTVLIPDKPVSLRFLDRGSGHAA* 2439
CYP27A1 Tetraodon nigroviridis
chr2:17054633-17057366 at UCSC browser
One frameshift at &
Adjacent to BCS1L and ZNF142 as in humans
MEGLIFGNGSVSLYYRFLERNSNILFFFKYSPLREQ
MWWKGSRLLPRPLVGPRVYSREAGQVFTAPTPPSKPRTLEDLPRVSLWELLYRLTIQGFHSRTHELQ (0)
ILEKERHGPIYRNGMKAVSICTATLLEEVLRNDDKFPNRGDMSIWKEYRDL
RGYGYGPFTE (2)
NEDRWYNLRVVLNKRMLHPRDALQYGGTLSEVVTDFIGRIYLLRQRT &
PTGDVVADLNNELYRFSLE (1)
AIASILFETRLGCLEEKIPTGTQDFISAISQMFSNNLQVFLMPRWSRGLLPYWRRYVAGWDGIFSF (1)
ATKLIDRKMEFIQQRLDDNQNVEGEYLTYLLANAQMSIKDVYGSISELLLAGVDT (0)
TSNCLTWTLHLLSEYPQCQEILFKEVSTSVPAGRAPSAEEVTKMPYLRAVVKESLR (2)
MFPVVPMNGRVVADRDVMIGGYHFSKN (0)
TAFNFSHYAISRDEHTFPEAARFLPERWLRDGHRRGGPNAFGTIPFGFGVRGCVGRRIAELEMYLFLFH (0)
LMRHFEIKPDPQMGELKSICRTVLIPDKPLSLYFLDRRSGHAA*
CYP27A2 Fugu rubripes (pufferfish)
chrUn:122717928-122721737 on UCSC browser
or Scaffold_697
41% to 27A1 mouse, 49% to 27A3 Fugu, 42% to 27A1 Fugu
38% to 27C1 Fugu
adjacent to MAP3K2 and SUMO1
note: MAP3K2 is next to ERCC3 in humans that is adjacent to CYP27C1
Fugu 27C1 is adjacent to ERCC3
Therefore CYP27A2 and CYP27C1 had a common origin
29906 MASFTALRCAAIGARNSALRPATLPSRNLNLQATSEAANLKGIADLPGPNTYKILYWLFVKGYGERSHLLQ 30118 (0)
30735 GKLKNIYGPMWRWKLGPYDFVSVASPELIARVIQQEGRYPVRVQLPHWKEYRDLRGQAYGLHVE 30926 (2)
31027 TGPEWSRLRSALNPRMLKLREVVAFSPVIHEVVGDLLRRIEFLRSGSQDGVTVSDIASELYKFGFE (1)
31334 GISAILFETRLGCLGEKVDPNVQRFISGVNDMLSLSDITYLFPRWTRSFVPVWKRFAQAWDDISDV 31531 (1)
31614 ASSLIDRRIAEIDARVANGQSVEGLYLTYLLSSDKMSRAEISTCITDLLLGGVDT 31778 (0)
32763 TSNTLSWALYHLAKDPVAQDRLYDEVNSVCPNHHQPTTDDLANMPFLKAVIKEVLR 32930 (2)
33004 LYPVVHQNARFISENDVILNDYWFPKK (0)
TQFHLCHYSVCHDETQFKHAERFLPERWLRHSAPLSGYYQHHPYSFIPFGVGVRACVGKRVAELEMYFALTR (0)
LMQSYNVQLEDDTMVVEPRTRTLLIPSKPINLRFLRRPGEQRC*
CYP27A2P Tetraodon nigroviridis (freshwater pufferfish)
GSCT00017845001_prot length=460
chr3:953742-956387 UCSC browser
adjacent to MAP3K2 and SUMO1
frameshift and 11 aa deletion at &1
frameshift at &2
73% to CYP27A2 Fugu
note in zebrafish, MAP3K2 is adjacent to CYP27C1, ERCC3
MASVAALRSAPIRPRARFPAALPRKELNLQATTRPAGLKGLEDLPGPGLG
RVLSWLFAEGYAEKSHLLQAQLRSLYGPMWRWTLGPYDLVGVAAPELIAG
IIQQEGRYPLRAPLPHWKDYRDLRGQAYGLHVDTGPEWSRLRSALNPKML
KLQEVATFAPVVHSVVGDLLRRLEFLRSGSQDGVTVSDVASELYKFGFEG
ISAILFETRLGCLDQKVDPNVQRFITAIGDMLSTSDFAYLVPRWARSLVP
VWKRFVQAWDDISDVASWLIDKRIAEIEAQVAGGRSVEGLYLTHLLSSNK
LSRAEIYTCITELLLGGVDTTSNTLSWALYHLAQDPGAQQRL &1
GEPTTEDLASMPFLKAVIKEVLR (2)
LYPVVPSNARVITENDVILGGYWFPKG (0)
TLYHLCHYTISHDASQFVHVEKFLPERWLRGGASQSRCYQHHPYSSIPFG
VGVRACLGRRVAD &2
LEMHFALTR (0)
LMQAYEVRLKDETEVVEAKTRTLLVPSKPINLRFLARA*
CYP27A3 Fugu rubripes (pufferfish)
No accession number
Scaffold_6002 Length = 16767
= LGS139924.x1 57% to 27A1 I-helix
= LGS125183.x1 Cyp27a1
First 16 aa and 49-81 supported by EST from AU050037 Paralichthys olivaceus
aa 49 on also supported by AW343479 zebrafish EST
in pufferfish 27A3 is between EAF2 and SLC15A2
these two genes are adjacent in humans but 27A3 is gone.
MFRNRLLTVGLRASVPHREGLHRTAVNYAGARRRHASSAATEITEHNVR
QKTMEDLGGPSFLTTLNWLFLKGYLPKTQQMQ (0)
4619 VEHSKIYGPLWKSKYGPMVVVNVASADLIEQVLRQEGRHPVRTDMPHWRRYRALRNQAHGPLTE 4428
MGAKWQRIRSILNPRMLKPQHVSSYGITINDVVTDFLEKLVWLRAKDGGGVMVNDVAGELYKFAFE
GISSVLFESRMGCLNDEVPEETQKFIYSVGEMFRLSAVVVLFPQSVWPYLPLWKKFVAAWDYLFKV (1)
1891 AEQMVQKKMEEIQNKVDLHQDVEGAYLTHLLLSEKMTVTEILGSITELLLAGVDT 1715
1661 TSNTISWALYQLAQNPSIQDQLYHEVRSVCPGNKMPDSDDIAQMPYLKAVIRETLR
LYPVVPGNARVTVDKEIVVGGYLFPKQ (0)
754 TLFHLCHYCVSHDENIFPNSRVFQPERWLRGREEKSKQHPFGSVPFGFGVRACLGRRV AELEMYLLLSR (0)
LIRRFEVRPDPNGAEVKPITRTLLCPATPINLQFLDRGAQRAPGPAAGASL*
CYP27A fragment a Fugu rubripes (pufferfish)
No accession number
a = exon 4 of 27A3
LPC42076.x1 39% to 27A1 202-276 79% to LPC42075.x1 gene duplication? Exon 4
GISSVLFESRMGCLNDEVPEETQKFIYSVGEMFRLSAVVVLFPQSVWPYLPLWKKFVAAWDYLFKV
CYP27A fragment b Fugu rubripes (pufferfish)
No accession number
Similar to exon 4 of 27A3
LPC42075.x1 35% to 27A1 79% to LPC42076.x1 gene duplication? Exon 4
GISSVLFESRLGCLNDEVPEETRRVIYSVGEMCRLSAVVVLFPQSGWPYLPVWARLGAAGDYLLQF
CYP27A3 Tetraodon nigroviridis (freshwater pufferfish)
chr2:18232734-18236941 UCSC browser
between EAF2 and SLC15A2
MLRSRLLSAGPARQGLRRAAASSAGARPRHAAPASSGVAGGQKTIDDLGGPSFLTTLNWLFVKGFLPKMQQMQ (0)
VEQRKIYGPLWKSKYGPLTVVNVASADLIEQVLRQEGRHPVRTDMPHWRRYRELRNQAHGPLTE (2)
MGAKWLRIRSILNPRMLKPKHVSSYASTINQVVTDFIQRVLWLGAQAGGGVMVHDVAGELYKFAFE (1)
GICSVLFENRMGCLNEEVPEETQKFIFSVGEMFRLSPLVVLFPKYMWPYLPFWKQFVQAWDYLFKV (1)
AEELVHKKMEEIQNKVDLQQSVEGAYLTHLLLSEKMSVTEILGSITELLLAGVDT (0)
TSNTISWSLYQLAQNPDVQERLYQEVSAVCPGDQLPNSDHIAQMPYLKAVIRETLR (2)
LYPVVPGNARVTVDNEIMVGDYLFPKQ (0)
TLFHLCHYCVSHDESVFLNSHAFQPERWLRGTEEKSKQHPFGSVPFGFGVRACLGRRVAELEMYLLLSR (0)
LIRRFEVRPDPAGTEVKPITRTLLCPAKPINLQFLDRGAGQTPRPGAGASPEPL*
CYP27A3 Danio rerio (zebrafish)
NP_001116749
4 aa diffs to seq on chr9:16358844-16371887 UCSC browser
EST DR721514.1, DN833321.1, DT066990.1, CD283382.1,
CR928406.1, CD599037.1, DT059155.1
These cover the whole seq with about 2 aa diffs
69% to CYP27A3 Fugu probable ortholog adjacent to EAF2 in both
MFSNSLLRTGLFLCKQDCSGIKCLTSMFLNARTTSTLIGGDKQKTMDDLD
GPSFLTSLYWLFGKGYFQTTHQMQIEHSKIYGPLWKSKYGPLVIVNVASA
DLIEQVLRQEGRHPIRTDMPHWRGYRKLRNHAYGPLTEMGPEWQRIRSIL
NPRMLKPKHVSNYTNAINGVVSDFIEKMAKLKTTKGNDVMVYDVAGELYK
FAFEGISSVLFETRMGCLNDEIPEETQKFIFSVGEMFRLSPIVILFPKFL
WPYMPFWKHFVAVWDHLFKVADELVQQKMTEIQEKVKTGSPVEGEYLTHL
LISEQMSFTEVLGSITELLLAGVDTTSNTISWALYHLAREPEIQQKLHEE
VVSVCPGDKTPSSDDITRMPLLKAIVRETLRLYPVVPGNARVVAENEIVV
GDHLFPKNTLFHLCHFAVSYDETVFPDPFAFLPQRWIREQKQLSQHPFGS
VPFGFGIRACLGRRVAELEMYLLLSRLIKRYEVRPDPSGKTVKPITRTLL
VPATSIDLQFIDR
CYP27A3 Oryzias latipes (medaka)
chr21:6587265-6596897 UCSC browser
adjacent to EAF2
MLRRPLLTATKRGASLLPRDDATKVAAAAGGAPSMLLAELRRHQTSPAVGVDKVKT
LDDLGGPSFLTTLNWLFLKGYFKKTQQLQ (0)
IEHRNIYGPIWKSKYGPLVVVNVASADLIEQVLRQEGKYPVRSQMPHWRSYRELRSKSHGPLTE (2)
LGAKWQRIRSILNPRMLKPVHVSSYTNTLNDVVTDFIERISWLRATGDQGITVNDLTGELYKFAFE (1)
GICSVLFEARMGCMNEVVPEETQKFIFSVGEMFRLSPIVVLFPKVFWPYLPAWKQFVATWDHLFSV (1)
AEDLVKKKMEEIQQDVETNKEVEGAYLTHLLLSDQMTVTEILGSMTELLLAGVDT (0)
TSNTISWALYHLAKEPEIQTKLYREVIDVCPGDKVPTSSDISQMPYLKAVIRETLR (2)
LYPVVPSNARVTVENDIIVGGYVFQKQ (0)
TLFHLCHYAVSHDESIFPDPHTFLPQRWLRGLDDKIKQHPFGSVPFGFGVRACLGRRVAELEMYLLLSR (0)
LLKSYEVRPDPAGTEVKPITRTLLCPAEPINLQFLDRKA*
CYP27A3 Gasterosteus aculeatus (three-spined stickleback)
chrUn:42545325-42552979 UCSC browser
adjacent to EAF2
note EAF2 and SLC15A2 are present in lizard
but no CYP27A3 is between them (lost on the way to tetrapods)
MLSRSTASLGLRLQRNATRSLLAALSPGPHGG
RHASASTSSTVTAHSSPLKTMEQLGGPGLLTTLNWLFVKGYFQTTQQMQ (0)
IEHSRIYGPLWKSKYGPLVVVNVASAQLIEQVLRQEGRLPVRTDMPHWRSYRELRNQAHGPLTE
MGVKWQRIRSILNPRMLKPKHVSSYACTINEVVGDFVRRAAWLRETGGGGLMVNDLTAELYKFAFE
GICSVLFETRMGCMNEVVPEETQKFIFSVGEMFRLSPILVLFPKSFWPYTPFWKQFVAAWDHLFKV
VAEELVQQKMDEIQEKVHLDQSVEGAYLTHLLLSEQMTVTEILGSITELLLAGVDT
TSNTIAWALYQLAKQPEIQEQLYQEVIGVCPGDKVPSSEDIAQMPLLKAIIRETLR
MYPVVPGNARVSVESEIVVGDHLFPKN
TLFHLCHYAVSYDESIFPDAHSFLPDRWLRGGEDKPRLHPFGSVPFGFGIRACLGRRVA ELEMYLLLSR
LMQRFEVRPDPAGAPVKPITRTLLCPATPINLQFVDRRPSLC*
CYP27A3 Osmerus mordax (Rainbow Smelt)
EL545126 EST
75% to 27A3 zebrafish N-term
MIGKCLLKIGLRACSQENCGIIRVTTTLNNVRDMRRGSSTAA
TTVIAGNDKQKTMDDLDGPSFMTSLYWLFGKGYFQTTQQLQIEHSKIYGPLWKSKYGPLV
IVNVASAELIEQVLRQEGRHPVRTDMPHWRAYRELRNQAYGPLTEMGAKWQRIRSILNPR
MLKPKHVSAYTNAINEVVTDFIDKVAWLRDTRGGGVMVNDLSGELYKFAFEGICSVLFET
RMGCLNEKVPEETQKFIFSVGEMFRLSPIVILFPKSVWPYLPFWKHFVVVWDHLFKIAEE
LVH
CYP27A4 Danio rerio (zebrafish)
BX321915.3, BC055637 60% to 27A1 fugu
Temp name CYP27A.d
2 aa diffs to chr9:32649613-32658960 UCSC browser
65501 MAVCFALSSAERLGIGLGFLRPTAAATGFRRAAGNAAAASVSVQDGHRK
LKTEADLPEIKIFTMLYRLLFKGYLSRMHELQ (0)
LYEKQLYGPLYKINVGNFQSVALNSVDLLEELLRKDEKFPSRGDMTLWTEYRDMKGIGYGPFTE
EGEKWYKLRAVLNKRMLHPKDSVQYGDVVNAVITDFIKRIYYLREMSPTGDLVSNLTNKLYRFSLE
GISSILFETRIGCLEKEIPAETQHFINSIAQMLTYSMPVVFMPNWTRNYLPFWQRYIGGWDGIFK
FAGKMIDMKMEALQKRVDANQEVAGEYLTYLLSNGKMSSKDVYGSVSELLLAGVDT
TSNTMLFALYHLSKDPETQDILYQEVTNVLKDDRIPTAQEVNTMSYLKAVIKETLR
LYPVVPMNARLIAENEVVIGGHLFPKN (0)
TTFTLCHYAISRDEKVFPEPQKFKPERWLRDGRTRPNPFGSIPFGFGVRGCVGRRIAELEMYLALAR
LIKLFEIRPDPTVGEVRSLNRTVLAPDRKVNLHLVERKKTEA* 56151
CYP27A5 Danio rerio (zebrafish)
BX321915.3
Temp name CYP27A.c
100% to chr9:32660646-32665857
59% to 27A1 fugu
54468 MAVCFALSSAERLGWCFLRPTAAATGFRSAAGNAAAASVSVQDGHRKLKTEADLPEI 54298
54297 KIFTMLYQMLFKGYLNSVHELQ 54232 (0)
LYQKQVYGPLWKINAGNLQGISISSVELLEELLRKDEKYPCRGYMTLWTEHRDLRGISYGPFTE 53897 ()
EGEKWYKLRAVLNKRMLHPKDSVQYGDVVNAVITDFIKRIYYLREMSPTGDLVSNLTNELYRFSLE
XIASILFETRIGCLEKEIPAETQKFINFIAQMFTYSMHVALLPNWTRKYFPFWQKYIDGWDGIFKF
GTKMIDMKMEAIQKRVDTNQDVAGEYLTYLLSNGKISRKDVYGSVSELLLAGVDS
TSNTMLWALYLLSKDPEAQETLYQEVTKVLKDDRIPTAEEVNSMPFLKAVIKETLR
LYPVVPVNSRLIAETEVVIGEYFFPKK (0)
TTFTLCHYVISRDEKVFPEPQKFKPERWLRDGRTRPNPFGSIPFGFGVRACVGRRIAELEMHLALAR
LIKLFEMRPDPTVGEVKANFRSVLVPNKKVNLHFVERQKTET* 49254
CYP27A6 Danio rerio (zebrafish)
BX321915.3
Temp name CYP27A.b
100% to chr9:32668191-32674283 (+)
61% to 27A1 fugu
46923 MAVCFALSSAERLGFGFLRPTAAATGFRRAAGNAAAASVSVQDGHRKLKTEA 46768
46767 DLPEIKIFTMLYQMLFKGYLNSIHELQ 46687 (0)
46408 IYQKQVYGPLSKINSGNLQGISISSVELLEELLRKDEKYPCRGYMTLWTEHRDLRGIGYGPFTE 46217 ()
EGEKWYKLRAVLNKRMLHPKDSLQYGDVVNAVITDFIKRIYYLREMSPTGDLVSNLTNELYRFSLE
XIASILFETRIGCLEKEIPVETQEFINSIAQMFTYSMHVALLPNWTRNYFPFWQKYIDGWDGIFKF
GTKMIDMKMEAIQKRVDTNQEVAGEYLTYLLSSGKMSSKDVYGSVSELLLAGVDT 1183
TSNTMLWALYLLSKDPAAQETLYQEVTKVLKDDRIPTAEEVNSMPFLKAVIKETLR 1000
LYPVVPVNSRLIAETEVVIGEYFFPKK (0)
TTFNLCHYAISHDEKVFPEPQKFKPERWLRDGRTRPNPFGSIPFGFGVRACVGRRIAELEMHLALAR
40957 LIKLFEMRPDPTVGEVKANFRSVLVPNKKVNLHFVERQKTET* 40849
CYP27A7 Danio rerio (zebrafish)
BX321915.3
Temp name CYP27A.a
100% to UCSC browser chr9 32687123-32697779 (+) strand
59% to 27A1 fugu zfishG-a34c11.p1c
28992 MAVSFALSSAERLGWCFLRPTTAATGFRRAAGNSAAASVSVQDGHRKLKTEA 27837
27836 DLPEIKIFTMLYQMLFKGYLNSVHELQ 27756 (0)
25958 LYQKQVYGPLWKINAGNLQGISITSVELLEELLRKDEKYPCRGYMTLWTEHRDLRGISYGPFTE 25767 ()
EGEKWYKLRAVLNKRMLHPKDSLQYGDVVNAVITDFIKRIYYLREMSPTGDLVSNLTNELYRFSLE
GIASILFETRIGCLEKEIPAETQEFINSIAQMFTYNMHVALLPNWTRNYLPFWQKYIDGWDGIFKF
GTKMINLKMEAIQTRLDTNQEVAGEYLTYLLSSGKMSCKDVYGSVSEVLLAGVDT
TSNTMLWALYLLSKDPAAQETLHQEVTKVLKGDRIPTAEEVNSMPFLKAVIKETLR
LYPVVPVNSRLIAESEVIIGEYLFPKK (0)
TTFNLFHYAISHDEKVFPEPQKFKPERWLRDGRTRPNPFGSIPFGFGVRACVGRRIAELEMHLALAR
LIKLFEMRPDPTVGEVKANFRSVLVPNKKVNLHFVERQKTET* 17333
CYP27A8-de4b5b Xenopus tropicalis (frog)
pseudogene
exons 4 and 5 only
scaffold_77:1644664-1645797 (-) strand UCSC browser
FETRIGCLDKQIPPETQKFIDSIGYMFKNSVYVTFLPHWTRGILPYWDRYIEGWDNIFDFGKR
LIDKKMSEIQSRLDKGEEVEGEYLTYLLSSANLTMGEVYGSVCELLLAGVDT
CYP27A8 Xenopus tropicalis (frog)
DR832386.1 CX969640.1 DR852196.1
old CYP27A1 Xenopus
best X.tropicalis hit to CYP27A1 hum in ESTdb
best match in human = CYP27A1 55%,
probably a CYP27A1 ortholog
scaffold_77:1626758-1638467 (-) complete in UCSC browser
Trace archive 570051728(+), walked to 411568263(+) 494948503(+)
54% to 27A7 zebrafish, 50% to 27A3 zebrafish, 50% to 27A1 human
this seq is near BCS1L and ZNF142 like human CYP27A1
MPSASKLGFLPLGRCRWLLHTGRGVSVSQGRAVAGAAVGAVGEEKKMKTF
EDLPGPSLLTNIYWVFLRGYILYTHELQAIYKKNYGPMWKST
LGRYKTVNIADVDILETVLRQEGKYPMRSDMEVWKEHRRQRDLSLGPFTEEGHKWHTLRS
VLNKRMLKPAEAMLYTGVVNEVVTDFLVRLEEMRSETPSGDMVNDIPNALYRFAFEGISY
ILFETRIGCLEKQIPVETQRFIDSIGAMLKNSIFVTIFPPWTNNLLPYYKRYMDSWDNIF
AFGNKLINEKMKKIEARLERDEEVQGEYLTYLISSGKLTDKEIYGSVAELLLAGVDTTSN
TLSWALYHLAREPEIQNALYQEVIGVVPGQNIPTSEDISSMPLLRAVIKETLR
LYPVVPTNSRVAVEKAITIGDYYFPKD
TLIALHHYHISRDEKNFPESDKFIPQRWFRESRVKNNPFSSIPFGYGVRACVGRRI AELEMHMCLSR ()
IIKKYEVRPDPSGAEIKSMARIVLTPHKPINLRFLPR
CYP27A9 Xenopus tropicalis (frog)
AL787054.2 DT421274.1 CR427561.1 BC094536.1
note there are three adjacent CYP27A genes in Xenopus
50% to 27A1 human, 42% to 27B1 human and 38% to 27C1 human
52% to 27A5 zebrafish, 51% to 27A3 zebrafish
scaffold_77:1590575-1599619, 11 aa diffs in first half
exon 6 missing in genome assembly
MRKGCHALLWKTCWANVQTGRE
KATLGVAGAVAEQEKKLKMSTDLPGPSTLNILYWVFLRGYVFESHKLQVIWKKRYGPLW
KTCIGSHRLVNVASPELLETLLRQEGKYPMRTDMFMWKEHRDLQDFSYGPLTEEGHRWHT
LRRVLNQRMLKPKEAVRYTESFNDVVTDLLVVIKEITAQSPNRTTVDGVANLMYKFAFES
ICTVLFETRIGCLKKEIPPETEKFINSIAIMLENQTRMEKLPRWTRGIFPYWRRFVEGWD
NIFIYGKKLIDKKMEEIEGRLKRGEEVEGEYLTYLLSSGKLSMEEICGSVAELLQAGVDTTSNT
LTWALYQLARNPEIQHNLHQEVIGVTPGDTIPDSEAIARMPLLRAVIKETLRLYPVVPEN
GRVVTEKDVILNDYIIPKNSQFVLCHYALSRDETQFPEPDRFLPERWLR
DSGMKHHPFSSIPFGYGVRACAGRRIAELEMHLALSRIIKMFQVVPDPELGEVGTKNRTV
LVSSRPINLQFIER*
CYP27A10 Xenopus tropicalis (frog)
CX393015 DR862140.1 DN030991.1
49% to 27A1 human
scaffold_77:1580461-1588306 complete in UCSC browser
53% to 27A7 zebrafish, 48% to 27A3 zebrafish
MIAQRLQTGA
QALLQQSCRASVQTVRKKATLGVSGATVVEGKTLKTLDDLPGPSPLKLLYWIFLRGYLFR
THELQVIFRKTYGPMWKMSDRQHAMVTVASPDLLESLLRKEGKYPTRADMFIMREHRDLR
GHSYGPVTEEGHQWHRIRTILNQRMLKPRETVVYAGSMNEVVSDLLLKIKELTAQSSSGT
QVNGVAELMYKFAFESICTVLFETRLGCLNKEILPETQKFIDSIGIMLEHLTMLTRLPQW
TKGILPYWGRYIEAWDTIFDFGKKLIDKKMEDIEGRLKRGEEVEGEYLTYLLSSGKLSME
EVYGSVVELLQAGVDTTSN
TLTWALYQLSRNPEIQNNLYQEVIRVIPGETIPDSEAIARMPLLKAVIKETLRL
FPVVPENARMINEKEVTIKDYVFPVKTQFILGHYAISRDETTFPEADRF
LPERWLRDSGMKHHPFGSIPFGYGVRACVGRRIAELEMHLALSRIIKMFQVIPDPDLGEV GAKNRAVLVANRPVNLRFIERQPRPE*
CYP27A11 Petromyzon marinus (Lamprey)
Contig1559:9792-24354 at UCSC browser, EST FD706879.1
adjacent to RQCD1 which is near CYP27A1 human
(about 7 genes away)
This is probably the ortholog of CYP27A1
51% to 27A3 zebrafish, 46% to 27A1 human, 48% to 27A5, 27A6 zebrafish
MAAWWTRVTRLRPLSLLLRAAPPGFARVAGTETRSRSWTETRAETRALRWAH
GSVAPVAAPVAAPRGAEHSRSPDELTGPSFFTIIYWVLCRNNNAHLLQ (0)
VECKNRYGPMWKSKFGPYMVISVADADLIEQVIRQEGKYPIRFDMKNW
TDYRDLRGYAYGPFTE
NGSKWNQLRSVLNQKMLRPRDMVAYVKPINEVVTDLLARLHMLSASNGGMVNNLANEFYKFAFEGTEAIL
FEKRLGCLEQSVPEETQVFIDAVRTVFIHSEKMALIPKWIRMILP
LWKHYCNAWDVIFSF
GMKEIDKKMIKISEMVARGEPVEGEYLTYLLSNGKLTEHEIHGSIAELLMAGIDT (0)
TSNTMSWVLYHLSREPHLQERLHQEVEGVCPGNTIPNVQDLAKMSFLKAVIKEILR (2)
LYPVVPSNSRLIVEGGTKVGGYVFPKG ()
TLFQLCHYAVSHNPEIFPDPEAFKPERWLRKGTMLAHPPFGSIPFGFGVRACVGRRVAELEMYLTLSR ()
LIKEFEVQWDPSQKDILPKTSTVIIPSESINLKFIARK
27B Subfamily
CYP27B1 rat
GenEMBL AF000139
St-Arnaud,R., Messerlian,S., Moir,J.M., Omdahl,J.L. and
Glorieux,F.H.
The 25-hydroxyvitamin D 1-alpha-hydroxylase gene maps to the
pseudovitamin D-deficiency rickets (PDDR) disease locus
J. Bone Miner. Res. 12, 1552-1559 (1997)
previously named CYP40. This sequence has errors in it.
CYP27B1 rat
GenEMBL AB001992
Cloning and expression of rat 25-hydroxyvitamin D3-1-alpha-
hydroxylase cDNA .
Shinki, T., Shimada, H., Wakino, S., Anazawa, H., Hayashi, M.,
Saruta, T., DeLuca, H.F. and Suda, T.
Proc. Natl. Acad. Sci. USA 94, 12920-12925 (1997)
Note: this sequence has been called CYP27B1 in this paper. The name
CYP40 was
given in May 1997 based on the sequence from John Omdahl that was
not
completely accurate at the time of submission for a name. It was
necessary to
revise the name CYP40 to CYP27B1.
CYP27B1 mouse
GenEMBL AB006034
Takeyama, K-i., Kitanaka, S., Sato, T., Kobori, M., Yanagisawa, J. and
Kato, S.
25-hydroxyvitamin D3 1 alpha hydroxylase and vitamin D synthesis.
Science 227, 1827-1830 (1997)
Cyp27b1 mouse
GenEMBL AB006034 AC084293.2 Mm.6216
LEADSPNTDMTQAVKLASRVFHRIHLPLQLDASLGSRGSESVLR
SLSDIPGPSTLSFLAELFCKGGLSRLHELQVHGAARYGPIWSGSFGTLRTVYVADPTL
VEQLLRQESHCPERCSFSSWAEHRRRHQRACGLLTADGEEWQRLRSLLAPLLLRPQAA
AGYAGTLDNVVRDLVRRLRRQRGRGSGLPGLVLDVAGEFYKFGLESIGAVLLGSRLGC
LEAEVPPDTETFIHAVGSVFVSTLLTMAMPNWLHHLIPGPWARLCRDWDQMFAFAQRH
VELREGEAAMRNQGKPEEDMPSGHHLTHFLFREKVSVQSIVGNVTELLLAGVDTVSNT
LSWTLYELSRHPDVQTALHSEITAGTRGSCAHPHGTALSQLPLLKAVIKEVLRLYPVV
PGNSRVPDRDIRVGNYVIPQDTLVSLCHYATSRDPTQFPDPNSFNPARWLGEGPTPHP
FASLPFGFGKRSCIGRRLAELELQMALSQILTHFEVLPEPGALPIKPMTRTVLVPERS
INLQFVDR
The mouse and probably rat have lost 27C1 due to a chromosome rearrangement between BIN1 and ERCC3
It seems that 27C1 was broken and lost in this event.
In fugu CYP27C1 is on the minus strand of scaffold 106 from 31916-36680 the
neigboring gene, 2624 bp away, is ercc3 at 39305-43553 minus strand (also found in human 39kb away)
Therefore, this linkage is very old. The next gene in the series ERCC3, CYP27C1 is BIN1 in human.
In mouse chr 1 is syntenic to human chr 2 at ERCC3, but BIN1 is on chr 18, implying a chromosome break.
CYP27B1 human
GenEMBL AB005989 cDNA sequence
Takeyama,K., Kitanaka,S., Sato,T., Kobori,M., Yanagisawa,J. and
Kato,S.
25-Hydroxyvitamin D3 1alpha-hydroxylase and vitamin D synthesis
Science 277, 1827-1830 (1997)
CYP27B1 human
GenEMBL AB005990 gene sequence
Murayama,A., Kitanaka,S., Takeyama,K. and Kato,S.
Human 25-hydroxyvitamin D3 1alpha-hydroxylase gene
Unpublished (1997)
CYP27B1 human
GenEMBL AB005038 cDNA sequence AB006987 gene sequence
Monkawa,T., Yoshida,T., Wakino,S., Shinki,T., Anazawa,H.,
Deluca,H.F., Suda,T., Hayashi,M. and Saruta,T.
Molecular cloning of cDNA and genomic DNA for human
25-hydroxyvitamin D3 1alpha-hydroxylase
Biochem. Biophys. Res. Commun. 239, 527-533 (1997)
CYP27B1 human
GenEMBL AF020192 cDNA sequence
Fu,G.K., Lin,D., Zhang,M.Y., Bikle,D.D., Shackleton,C.H.,
Miller,W.L. and Portale,A.A.
Cloning of human 25-hydroxyvitamin D-1alpha-hydroxylase and
mutations causing vitamin D-dependent rickets type 1
Mol. Endocrinol. 11, 1961-1970 (1997)
CYP27B1 human
GenEMBL AF0027152 gene sequence
Fu,G.K., Portale,A.A. and Miller,W.L.
Complete structure of the human gene for the vitamin D 1alpha-
hydroxylase,
P450c1alpha.
DNA Cell Biol. 16, 1499-1507 (1997)
CYP27B1 human
NM_000785
MTQTLKYASRVFHRVRWAPELGASLGYREYHSARRSLADIPGPS
TPSFLAELFCKGGLSRLHELQVQGAAHFGPVWLASFGTVRTVYVAAPALVEELLRQEG
PRPERCSFSPWTEHRRCRQRACGLLTAEGEEWQRLRSLLAPLLLRPQAAARYAGTLNN
VVCDLVRRLRRQRGRGTGPPALVRDVAGEFYKFGLEGIAAVLLGSRLGCLEAQVPPDT
ETFIRAVGSVFVSTLLTMAMPHWLRHLVPGPWGRLCRDWDQMFAFAQRHVERREAEAA
MRNGGQPEKDLESGAHLTHFLFREELPAQSILGNVTELLLAGVDTVSNTLSWALYELS
RHPEVQTALHSEITAALSPGSSAYPSATVLSQLPLLKAVVKEVLRLYPVVPGNSRVPD
KDIHVGDYIIPKNTLVTLCHYATSRDPAQFPEPNSFRPARWLGEGPTPHPFASLPFGF
GKRSCMGRRLAELELQMALAQ
ILTHFEVQPEPGAAPVRPKTRTVLVPERSINLQFLDR
CYP27B1 Bos taurus (cow)
See cattle page for details
MTQTLKFASRVFHRVRCPELGASLGSRGSESAPRVLADIPGPSTPGFLAELFCKGGLSRLHELQ (0)
VQGAARFGPVWLASFGTVRTVYLAAPTLVEQLLRQEGPRPERCSFSPWTEHRRRRQRACGLLTA (2)
EGEEWQRLRSLLAPLLLRPQAAARYAGTLHGVVRDLVRRLRRQRGLGAGPPSLVRDVAGEFYKFGLE (1)
GIAAVLLGSRLGCLEAEVPPDTETFIRAVGSVFVSTLLTMAMPSWLHRVVPGPWDRLCRDWDQMFAF (1)
AQQHVEQREAEVAMRNQSEKSEEDMGPGAHLTYFLLQKELPAASILGNVTELLLAGVDT (0)
VSNTLSWALYELSRHPEIQTALHAEITAALGPGSSTQPSATALSQLPLLKAVVKEVLR (2)
LYPVVPGNSRVPDRDICVGEYIIPKN (0)
TLVTLCHYATSRDPAQFPEPNSFRPARWLGEGPAPHPFASLPFGFGKRSCVGRRLAELELQMALAQ (0)
ILIHFEVQPEPGSAPVRPMTRTVLVPERSINLQFVDR*
CYP27B1 pig
DQ295065
MTQTLKLASRVFHRVCRAPELGSRGSDSAPRGLADLPGPSTPGF
LAELFCKGGLSRLHELQVQGAARFGPVWLASFGKVRTVYVAAPTLVEQLLRQEGPLPE
RCSFSPWTEHRRRRQRSCGLLTAEGEEWQRLRSLLAPLLLRPQAAARYAGTLHDVVQD
LVRRLRSQRGLGAGPPALVRDVAGEFYKFGLEGIAAVLLGSRLGCLEPEVPPDTETFI
RAVGSVFVSTLLTMAMPSWLHRLVPGPWARLCRDWDQMFAFAQEHVERREAEAAMKSQ
GKPEEDLGSGAHLTYFLFREELPAPSILGNVTELLLAGVDTVSNTLSWALYELSRHPE
VQMALHSEIKTALGPSSSAHPSATVLSQLPLLKAVVKEVLRLYPVVPGNSRVPDKDIC
VGDYIIPKNTLVTLCHYATSRDPAQFPEPNSFRPARWLGECPAPHPFASLPFGFGKRS
CMGRRLAELELQMALAQILIHFEVQPEPGSAPIRPMTRTVLVPERSINLQFVDR
CYP27B1 Xenopus tropicalis
NM_001006906.1
scaffold_67:1250110-1259521 in UCSC browser
this gene is between MARCH9 and METTL1 LIKE human 27B1
54% to human
63% to 27B1 zebrafish, 48% to 27A3 zebrafish, 54% to 27B1 human
MAQTLKLGSSRSSQLFRGLQELWAETVLKNSEKVIKGHKSLADM
PGPSTVSFISDLFCRRGLARLHELQLEGKAKFGPVWKASFGPILTVHVAEPSLIEQVL
RQEGKHPIRSDLSSWKDYRQCRGHSYGLLTAEGEEWQQFRSILGKHMLKPKEVEAYSD
VLNDVVGDLIKKINYQRSQNQNNVVKDIAKEFYMFGLEGISSVLFESRIGCLEPTVPK
ETEKFIQSINTMFVMTLLTMAMPKFLHKIFRKPWQKFCESWDYMFAFAKGHIDKRMKD
VAQKLAQGEKVEGKYLTYYLAQEKIPMKSIYGNVTELLLAGVDTISSTLSWSLYELAQ
HPDIQSAVYSEVEEILQGKQIPSPSDVARMPLLKAVVKEVLRLYPVIPGNARVVADRD
IQVGDYIIPKKTLITLCHYATSRDENVFSNPNEFQPDRWLKKEDTHHPYASLPFGFGK
RSCIGRRIAELEVYLALARILSHFEVKPEQPGSLVMPMTRTLLVPEKEINLQFLER
CYP27B1 Danio rerio (zebrafish)
chr11:1675158-1683318
72% TO 27B1 FUGU
568 MMMVLHQALKVTGRSALPLLRFAERWADVIRAPPTPQVKTLEQMPGPSPARFIRDLFMKRGFS 380
379 RLHQLQ 362 ()
337 LEGRQKYGPMWKASFGPILTVHVAEPELIQQVLRQEGQHPMRSELSSWKDYRALRGEG 164
163 YGLLT 149 ()
158 SEGEEWQCVRSLLSKHMLRPQAVEAYDGALNAVVSDLLQKLKLRSQESSSRIVSDISAEF 337
338 YRFGLEG 358 ()
281 ISSVLFESRIGCLDAVVPVETERFIQSINTMFVMTLLTMAMPQWLHRLLPKPWDTFC 451
452 RCWDVMFEF 478 ()
46 AKGHIDQRLQQEKQKLECGEQLEGRYLTYFLSQAGLPLTSVYSNVTELLLAGVDT 210
592 ISSTLSWSLYELSRHPDVQTALRXEVLSVMKGRRSVPQASDVAAMPLLKAVVKEIL 425 ()
345 RLYPVIPANARVINKDIEVGGYVIPKN 265 ()
84579 TLITLCHYATSRDP 84620
84620 QQFRDPDSFRPQRWGDRSDRSHPYATVPFGVGKRSCIGRRIAELEVYLALSR 84775
85167 ILMHFTVEPARQDDTVHPMTRTLLVPERQIDLRFTER* 85280
CYP27B1 Fugu rubripes (pufferfish)
No accession number
Scaffold_470
52% to 27B1 human
MLQQALRVSCRSASPLVKWMERWAECASARPQAVKPLGDMPGPSVASFAWDLFAKRGLSRLHELQ (0)
LEGVRRYGPMWKASFGPILTVHVADPALIEQVLRKEGQHPMRSDLSSWKDYRRLRGHHYGLLTS (2)
51430 EGEEWQSIRSLLGKHMLRPKAVEAYDQTLNSVVDDLITKLRLRRSSQGLVTDIASEFYRFGLE 51630 (1)
51726 GVSSVLFESRIGCLDKIVPEETERFIQCINTMFVMTLLTMAMPSWMHQLFPKPWNVFCQCWDYMFDF (1)
AKGHIDQRMAAEAEKIARGEEVEGRYLTYFLSRTSLPMKTVYSNVTELLLAGVDT (0)
52280 ISSTLSWSLYELSRHQAVQASLREEVLSVLGGRRVPTAADVAQMPLLKATIKEVLR 52444 (2)
52527 LYPVIPANARVITERDIQVGGYLIPKN 52610 (0)
52697 TLITLCHYATSRDPAVFPRPDEFLPQRWLNKEQSHHPYASVPFGVGKRSCIGRRIAELELYLAVAR 52894 (0)
53237 ILLEFDIKPDPEGISVKPMTRTLLVPENVINLQFTER* 53347
CYP27C1 human
GenEMBL AC027142
BM562765 BI459427 ESTs
43% identical to 27A1 assembled gene
intron starting with QIH ending in VDT is from Celera's data
CRA_Gene|hCG42613 /len=10487. This Celera sequence is still missing the C-terminal. Probable last exon is now found in AC027142. AG Intron boundary is in the same location as CYP26B1. Stop codon is one codon away from 26B1s stop codon. Length is preserved from cys to intron. (n) = intron phase, 9 exons
1 85452 MQTSAMALLARILRAGLRPAPERGGLLGGGAPRRPQPAGARLPAGARAEDKGAGRPGSPPG 85634 61
62 85635 GGRAEGPRSLAAMPGPRTLANLAEFFCRDGFSRIHEIQ (0) 85748 99
100 39574 QKHTREYGKIFKSHFGPQFVVSIADRDMVAQVLRAEGAAPQRANMESWREYRDLRGRATGLISA (2) 39371 163
164 43984 EGEQWLKMRSVLRQRILKPKDVAIYSGEVNQVIADLIKRIYLLRSQAEDGETVTNVNDLFFKYSME (1) 43787 229
230 41743 GVATILYESRLGCLENSIPQLTVEYIEALELMFSMFKTSMYAGAIPRWLRPFIPKPWREFC 41564 290
291 41563 RSWDGLFKFS 41534 300 (1)
301 QIHVDNKLRDIQYQMDRGRRVSGGLLTYLFLSQALTLQEIYANVTEMLLAGVDT (0) 354 (Celera sequence)
355 110201 TSFTLSWTVYLLARHPEVQQTVYREIVKNLGERHVPTAADVPKVPLVRALLKETLR (2) 110034 410
411 108566 LFPVLPGNGRVTQEDLVIGGYLIPKG (0) 108489 436
437 108006 TQLALCHYATSYQDENFPRAKEFRPERWLRKGDLDRVDNFGSIPFGHGVRSCIGRRIAELEIHLVVIQ (0) 107794 504
505 102503 LLQHFEIKTSSQTNAVHAKTHGLLTPGGPIHVRFVNRK* 102619 542
The mouse and probably rat have lost 27C1 due to a chromosome rearrangement between BIN1 and ERCC3
It seems that 27C1 was broken and lost in this event.
In fugu CYP27C1 is on the minus strand of scaffold 106 from 31916-36680 the
neigboring gene, 2624 bp away, is ercc3 at 39305-43553 minus strand (also found in human 39kb away)
Therefore, this linkage is very old. The next gene in the series ERCC3, CYP27C1 is BIN1 in human.
In mouse chr 1 is syntenic to human chr 2 at ERCC3, but BIN1 is on chr 18, implying a chromosome break.
CYP27C1 Bos taurus (cow)
See cattle page for details
MALLARILKARPSPAAGRTGLLGGWDAGQPRLASSGLSARARAEDKGAGRPGASQAGGR
AEGPRSLAAMPGPRTLANLVEFFGKDGFSRIHEIQ
297 QKHTREYGKIFKPHFGPQFVVSVADRDLVAQVLRAEGASPQRANMGSWQEYRDLRXRS 476
477 TGLISA 494
EGEQWLKMRSVLRQRILKPKDVAIFAGEINQVIADLIKRIYFLKSQAEDGDTVTNINDLFFKYSME
GVATILYESRLGCLGNSIPQLTADYIEALALMFCTFKTSMYAGAIPRWLRLLIPKPWQEFCRSWDGLFEFS
QIHVDNKLRDIRCQMERGERVRGGLLTYLFLSQELTLEEIYANMTEMLLAGVDT
TSFTLSWAVYLLARHPEVQQALYREIVRNLGERHVPTAADVPKVPLVRALLKETLR
LFPVLPGNGRVTQEDLIVGGYLIPRG
TQLALCHYATSYEDENFPRAKEFRPERWLRQGNLRRVDNFGSIPFGYGARSCIGRRIAELEIHLLVIQ
LLQHFEIKTSPWTKTVHAKTHGLLMPGEPIHVRFVNRK
CYP27C1 Gallus gallus (chicken)
GenEMBL BU235126 BU209688
Partial seq missing N and C-terminals
78% to Silurana 27C1 82% to human 27C1
PGLSGSQSLEVRSGAENKAARPGELLEPSPQLGRV KSLHEMPGPNTLYNLYEFFWKDGFGRIHE
IQQKHTQEYGKIFKSHFGPQFVVSIADRDMVAQVLRSEGRAPQRANMESWQEYRDLRGR
ATGLISAEGEQWLKMRSVLRQKILKPKDVAVYSGGVNEVITDLIKRIYTLRSQEEDGETV
TNVNNLFFKYSMEGVATILYECRLGCLENNVPQQTVEYIEALELMFSMFKTTMYAGAIPR
WLRPFIPKPWREFCRSWDGLFKFSQIHVDNKLKSIQS
QLDQGEEVNGGLLTYLLVSKELTLEEIYA
NMTEMLLAGVDTTSFTLSWAIYMLAKHPEVQQRVYEEIINKLGKDQAPVNRDVPKLPLIR
AVLKETLR
CYP27C1 Silurana tropicalis (frog)
GenEMBL BQ392731 AL629634 AL595312
74% to human 27C1 not counting the divergent N-terminal
87% to Xenopus 27C1
MAALGQLLRGSARLEGLARSFHRFPGAQAAGQALEHEQAEGVLGATVKGSPMVKN
LKEMPGPSTMANLVEFFWRDGFGRIQEIQQKHARQYGRIFKSHFGPQFVVSIADKDMVAQ
VLRAERDAPQRANMESWHEYRELRGRSTGLISAEGEKWLNMRSVLRQKILRPRDVAMYSG
GVNEVVEDLVKRIRKLRVQESDGLTVTNVNDLYFKYSMEAIATILYECRLGCLDDQIPQQ
TKEYIEALELMFSMFKTTMYAGAIPKWLRPLIPKPWREF
CRSWDGLFKFSQIHVDDRLRQIESQLEKGEEVQGGVLTHLLLSKELDLEEIYA
NMTEMLLAGVDTTSFTLSWATYLLAKNPGIQEAVYQQIVQNFGKDQVPTAEDVPKMPLVRAVVKETL
RLFPVLPGNGRVTQDDLVVGGYFIPKGTQLALCHYSTSYDAECFPAAEEFRPERWIRSGN
LERKENFGSIPFGYGIRSCIGRRVAELEMHLLLIQLLQ
NLEIKPSPQTTTVLPKTHGLLCPGGKINVRFVDRQ*
CYP27C1 Xenopus laevis (African clawed frog)
GenEMBL BJ086834 CA982419 BQ385474 BJ069739
87% to Silurana 27C1
MAVLGHLLKGSARLEGLARGFHQFPKIQAAGQALEQEQAEGEL
GARAKEAPMM KSLKDMPGPSTLANLVEFFWRDGFGRIHEIQQKHTRQYGRIFKSHFGPQF
VVSIADKDLVAQVIRAERDAPQRANMESWHEYRELRGRSTGLISAEGEKWLNMRSVLRQK
ILRPRDVAMYTGGVNEVIGDLVKKIHKLRAQESDGLTVTNVNDLYFKYSMEAIATVLYEC
RLGCLDDIIPQQTKEYIAALELMFSMFKTTMYAGGLPKWLRHHNFPNPWKEF
53aa gap see Silurana seq
HMTEMLLAGVDTTSFTLSWATYLLAKNPSIQESVYQQIVQNLGKDQVPTAEDVTKIPMVR
AVVKETLRLFPVLPGNGRVTQDDLVLDGYLIPKGTQLALCHYSTSYDDKCFPGAEEFKPE
RWIRSGYLERKENFGSIPFGNGIRSCIGRRVAELEIHLLLIQL
LQNFEIKLSPETMTVLPKTHGLLCPGEKINVRFLDRQ*
CYP27C1 Xenopus tropicalis
scaffold_893:261736-267439 exon 3 missing in a gap and
last half not on this scaffold
N-term adjacent to ERCC3 as in zebrafish and human 27C1
scaffold_4119:30-1794 has second half of gene 100% match
NM_001011341.1 short at N-term (58 aa) CX494774.2 68% to human
from refseq database
70% to 27C1 zebrafish
MAALGQLLRGSARLEGLARSFHRFPGAQAAGQALEHEQAEGVLGATVKGSPMVKNLKE
MPGPSTMANLVEFFWRDGFGRIQEIQQKHARQYGRIFKSHFGPQ
FVVSIADKDMVAQVLRAERDAPQRANMESWHEYRELRGRSTGLISAEGEKWLNMRSVL
RQKILRPRDVAMYSGGVNEVVEDLVKRIRKLRVQESDGLTVTNVNDLYFKYSMEAIAT
ILYECRLGCLDDQIPQQTKEYIEALELMFSMFKTTMYAGAIPKWLRPLIPKPWREFCR
SWDGLFKFSQIHVDDRLRQIESQLEKGEEVQGGVLTHLLLSKELDLEEIYANMTEMLL
AGVDTTSFTLSWATYLLAKNPGIQEAVYQQIVQNFGKDQVPTAEDVPKMPLVRAVVKE
TLRLFPVLPGNGRVTQDDLVVGGYFIPKGTQLALCHYSTSYDAECFPAAEEFRPERWI
RSGNLERKENFGSIPFGYGIRSCIGRRVAELEMHLLLIQLLQNFEIKPSPQTTTVLPK
THGLLCPGGKINVRFVDRQ
CYP27C1 Danio rerio (zebrafish)
ctg14633 zebrafish 70% TO FUGU CYP27C1
chr6:7837526-7850296
MARKNLLQESCRQLLIQTHGLHKSVASGSLEIAAHSQADLKEESAVSPAEEVQ
KAARVKSLKEMPGPSTVANLLEFFYRDGFSRIHEIQ 46230
MEHAKKYGKIFKSRFGPQFVVSIADRDMVAQV 48886
48887 LRSESATPQRGNMESWKEYRDLRGRSTGLISA 48982
EGDEWLKMRSVLRQLIMRPRDVAVFSSDVT 50683
50683 DVVADLVKRVKTLRSQQDDSQTVLNINDLFFKYAME 50790
GVATILYETRLGCLENEIPKMSQEYITALHLMFSSFKTTMYAGAIPKW 52654
52655 LRPIIPKPWEEFCSSWDGLFKFS 52723
52813 IHVDKRLSEIKKQMEKSEEIKGGLLTHMLVTREMNLEEIYANMTEMLLAGVDT 52971
55090 TSFTLSWSTYLLARHPTIQQQIFEEVDRVLGGRVPTGEDVPYLPLIRGLVKETLR 55254 ()
LFPVLPGNGRVTHDDLIVGGYLIPKG 55452 ()
58126 TQLALCHYSTSMDEENFPRPEEFRPDRWIRKDASDRVDNFGSIPFGYGIRSCVGRRIAEL 58305
58306 EMHLALTQ (0)
LLQNFHIEVSPQTTEVHAKTHGLLCPGASINLRFTDRK* 58539
CYP27C1 Fugu rubripes (pufferfish)
chrUn:4630123-4635029 UCSC browser
Scaffold_1410
75% to 27C1 human
adjacent to ERCC3
MSVMNKLTTTCWTNFYGDRRNKQMLFVLRCLHKSATSGTFGVAREEPLPERLITSTDATKKRLPKT
19233 LAEMPGPGTISNLFEFFWRDGFSRIHEIQ 19319 (0)
20076 IEHSKMYGKIFKSRFGPQLVVSVADRDLVAEVLRAE
GVAPQRANMESWHEYRDMRRRSTGLISA 20261 (2)
EGEDWLRMRSVLRQLIMRPRDVAVFSDDVSEVVDEVVDDLIKR
IVCLRSQSSDGTTICNINDLFFKYAME (1)
20741 GIAAILYECRLGCLSQKIPQETEDYIDALHLMFSSFKTTMYAGAIPKWLRPV 20911
20912 FPKPWEEFCDSWDGLFRF 20968 (1)
SVHVDKRLKQIESQLQRGEKVTGGLLTYMLVAKEMSVEEIYANVTEMLLAGvdt (0)
23004 TSFTLSWASYLLARHPDVQQQIHAEVMRVLGSEKVATAEDVQHLPFIRGLVKETLR 23183 (2)
LFPVLPGNGRITQDDMVLGGYFIPKG
23623 TQLALCHYSTSLDDENFPSSLEFRPDRWIRKHSSDRLDNFGSIPFGYGIRSCIGKRI 23793
23794 AELEMHLALIR (0)
23998 IIQKFHVCVSPLTTDVKAKTHGLLCPGAPINLQFIDREI* 24117
CYP27C1 Tetraodon nigroviridis
chrUn_random:116718046-116719086 UCSC browser
N-term exons 1 and 2 only, adjacent to ERCC3
63% to Fugu CYP27C1
rest of gene in a sequence gap
MSVMNKLTTVCWRNFQSDCHHRQMRFALRCLHKSATSGAFGAAREEAVPERLITPKDAAQKK
SGKSLAEMPGPGTVSNLIEFFWRDGFSRIHEIQ (0)
MEHSKMYGKIFKSRFGPQLVVSVADRDLVAEVLRAEGVAPQRGNME
SWHEYRDLRGRSTGLISA (2)
CYP27C1 mouse and rat
Mouse is missing this sequence in blast searches. In humans it lies between
BIN1 and ERCC3 on chr 2. This region does not contain 27C1 in mouse (chr 18)
unless there is a sequence gap. Blast of the rat genome
(Rat Genome Sequencing Consortium Assembly Posted date: Dec 4, 2002)
also had no hits, so 27C1 may have been lost in rodent evolution.
It is present in human, Fugu, Xenopus, Silurana (another frog) and chicken.
The absence of 27C1 may be a useful synapomorphy for comparing rodents to other mammals.
CYP27D1 Petromyzon marinus (Lamprey)
Contig17808:3625-10857 (-) strand
missing N-term exon in a sequence gap
This sequence is more like CYP27A3 or CYP27B1
47% to CYP27A3 Fugu
IIHHKRFGSMWTSYTGREMFVCLADPDLIEQLMRQEGPYPVRVIMSAWDDYLMMRGHSKGLLTQ (2)
TGADWKRMREVLNQRMLKPREVMQFADPLNAVVTELMGHVRARRGNGAATAVFPLPDVAQLLYRFAFE ()
GVTTVLFETRMGSLEETVPKETQDFIRAIESMLSTTVHVMALPRWTRPLLPFWGRFVQGWD
GSITAATRLINVKLAEVKDCISRGQEVRGEYLTYLLASDRLSLPEIYSNTTDILLAGVDT ()
TSNTLSWSLYHLAQMPALQEALCREVNAVVPGDAVPGIDEVARMPLLKAVIKETLR ()
LYPVVFQNARCIVDDGVVLGGYSLPKG (0)
TNLSLCQYVLSMDGDVFPNPREFQPQRWLRGKDRAAWRHGSGGEGEEGAAAADDAATSEK
THPFAYIPFGFGLRGCVGRRIAELEMHLCLAR (0)
FVKTFEMMPEPGAKKVLPKTRGVLVPSTPINLLLRERL
Traces from next contig upstream
All of these are in a long repeat sequence
Cannot find a mate pair seq that is outside of the repeat.
1193719280, 1255501914, 1472630360, 1470299497, 1467953899, 1466704577,
1443851513, 1447257494, 1196739470, 1266761373, 1466575034
1466508070, 1266008803, 1193867804, 1443531934, 1468578376
Similar gene exon 2, trace 1467681227 Petromyzon
DLNIRRNSYDHLEQLKLQCGGILTSYTGREMFVCLADPYLIEQLMRQEGPYPVRVI
MSAWHDYLMMRGHSKGLLTH
(mate = 1468976488 exon 5)
TSNTLS*SLYH
AQMPALQEAWCREVNAVV
PGDAAPGIDEVARMTLLKAVIKETLR
Vertebrates
CYP27E1 Branchiostoma floridae (Amphioxus)
40% to 27B1 Fugu, 37% to 27C1 fugu,
40% to 27A1 fugu (but not first exon)
35% to 11A1 fugu, 34% to CYP24 fugu (Best match to CYP27B)
MAQQILRNSSVCSLVRPNSRALVSVAPAATVQQNRPLKEMPGPTNKLGQLWWGFKNRSRMHEAQ (0)
LEQERKYGRMWQSSFGFNPNVNVAHVALAEQLMRQEGKYPKRIEVNFMQQYRDLRGYSYGLLNQ (2)
NGPEWRHLRTAVSKRIMRPKEVPR (2)
YGDSMNEVVTDMIDRFKDLRDTTGGGKTVPDLTNELYKWAME (1)
SIATVLFDTRLGCLEREMPEKTQQFIDSIATMFRTAFLVSALKPWMLTYLGLGVWKRHVEAWDVIFSV(1)
AHENIDRKVLDIDARLSRGEDLVGSFLTYMLTGTDVTKKDLYATVTELLLAGVDT (0)
TSNTMVWTLYELARHPELQERLHQEVTSVVSPGQIPTVDDVKNMALLKNVIKEILR (2)
VYPVLPANGRVLDKDIVLDGYNIPKG (0)
TQFAILHYNMTRDPEVFEEPDRFNPDRWTRMGTEKVNTFSSVPFGFGPRQCA (1)
GRRLAEMEMYLVLAR (0)
LVQTFEVRQLTPGEVVRPVTRALLVPGDPVHLEFIDRP*
CYP27F1 Strongylocentrotus purpuratus
XP_790546.2 CX682858.1 EST
CYP27B like 40% to 27A in Xenopus
Missing C-terminal
1 MATRLSRFLA RARPSRSSGV RPQTFHDAHA SSCPLAAVPS TLSPHASTTT PSTPSTTKTS
61 TTSTTSAPSS ISDLPSNNNL SLGVSNGCTF DQAPTSINPS ERNCTIFDDV KSFKDIPGPK
121 GWPILGTLGT YLSGKGLERI YDHQIDFTKK YGPIWKERLG TLEFVNLAKP DLVEVMYRND
181 SRYPRRLDMK PWKVYRAHRD ESLGVLTHEG KEWHRARRTI GRKIMPPREV AKFTGVINEI
241 VTDMVERLRF VRDTKGEGDG VVPELQNEMY KWSKGSIFKL LLETRIGCXK EPIPEKSQGF
301 INAVGQMFAS GQIMFLTSPE WFRRWNLKPW RDHEAAWDYI FNFAKKSVDA RLQELATLAE
361 EGDVVEDSGI LTHLLASQQL SMKEVYSNAC ELLLAGVDTT SNTLAWALYE LSRHPDTQDR
421 LAKEVEGALA AKGRCIPEHD DLPNMPLLKG VIKETLR
LYPVVPANNRVLDKDVTIGGYHIPKG
28A Subfamily
CYP28A1 Drosophila mettleri
GenEMBL U89746 (1895bp)
Danielson,P.B., MacIntyre,R.J. and Fogleman,J.C.
Molecular cloning of a family of xenobiotic-inducible drosophilid
cytochrome p450s: evidence for involvement in host-plant
allelochemical resistance.
Proc. Natl. Acad. Sci. U.S.A. 94, 10797-10802 (1997)
note: 369mt, full length, one of 109 seqs submitted to Nomenclature
committee
CYP28A2 Drosophila mettleri
GenEMBL U89747 (1764bp)
Danielson,P.B., MacIntyre,R.J. and Fogleman,J.C.
Molecular cloning of a family of xenobiotic-inducible drosophilid
cytochrome p450s: evidence for involvement in host-plant
allelochemical resistance.
Proc. Natl. Acad. Sci. U.S.A. 94, 10797-10802 (1997)
note: 43mt, full length, one of 109 seqs submitted to Nomenclature
committee
CYP28A3 Drosophila nigrospiracula (a desert dwelling Drosophilid)
GenEMBL U91565 (451bp)
Danielson,P.B., MacIntyre,R.J. and Fogleman,J.C.
Molecular cloning of a family of xenobiotic-inducible drosophilid
cytochrome p450s: evidence for involvement in host-plant
allelochemical resistance.
Proc. Natl. Acad. Sci. U.S.A. 94, 10797-10802 (1997)
54 amino acids
CYP28A4 Drosophila hydei (a desert dwelling Drosophilid)
GenEMBL U91566 (366bp)
Danielson,P.B., MacIntyre,R.J. and Fogleman,J.C.
Molecular cloning of a family of xenobiotic-inducible drosophilid
cytochrome p450s: evidence for involvement in host-plant
allelochemical resistance.
Proc. Natl. Acad. Sci. U.S.A. 94, 10797-10802 (1997)
54 amino acids
Cyp28a5 Drosophila melanogaster
GenEMBL AC018242 6065-8082 also AC001660
Cyp28a5 Drosophila pseudoobscura
See D. pseudoobscura page on this server for sequence
Obtained from UC Santa Cruz Browser
CYP28B1 Musca domestica (housefly)
no accession number
Nannan Liu
44% identical to 28A1 over 498 amino acids
submitted to nomenclature committee 7/29/99
Cyp28c1 Drosophila melanogaster
GenEMBL AC014191 comp(8254-10002) also AL133495
Cyp28c1 Drosophila pseudoobscura
See D. pseudoobscura page on this server for sequence
Obtained from UC Santa Cruz Browser
Cyp28d1 Drosophila melanogaster
GenEMBL AC017780 comp(28385-30262) also AC009355 AC008324 AC008327
Cyp28d1 Drosophila pseudoobscura
See D. pseudoobscura page on this server for sequence
Obtained from UC Santa Cruz Browser
Cyp28d2 Drosophila melanogaster
GenEMBL AC017780 comp(31545-33456) AC008324
CYP28E1 Lucilia cuprina (Australian blowfly)
No accession number
Zhenzhong Chen
Submitted to nomenclature committee March 17, 2005
Clone name Lc_CYP28D
48% to 28D1 (148 aa)
29A Subfamily
CYP29A1 Caenorhabditis elegans (nematode worm)
GenEMBL Z69787 and AL022276 (Y102F5)
C44C10.2 (with modifications)
note: GeneFinder translation is incorrect in some regions.
First exon probably ends at 11181 coding for 35 amino acids as does
the first exon
of CYP29A2.
There is probably a sequence error in the stop codon at 11155-11157.
2nd exon probably runs from 11227-11293.
11400-11518 is the third exon.
12465 to 12979 should be one exon.
There is probably a sequence error in the stop codon at 12879-12881.
some segments around I-helix missed by genefinder. N-terminal of
genefinder translation is probably a different gene. Matches
F23A7.3, but this gene
does not have a P450 sequence downstream.
CYP29A2 C. elegans
GenEMBL Z74043
T19B10.1
nearly the whole gene of wEST00713, CEMSH91R, missing 5' end
CYP29A2 C. elegans
GenEMBL Z74040
T19B10.1
5' end of gene from Z74043
CYP29A3 C. elegans
no accession number
Y108G3 contig 541
CYP29A4 C. elegans
GenEMBL Z99102
B0331.1
30A Subfamily
CYP30 Mercenaria mercenaria (northern quahog, a clam)
GenEMBL AF014795 (1628bp)
Brown, D., Clark,G.C. and Van Beneden,R.J.
A novel cytochrome P450 from the clam, Mercenaria mercenaria
Unpublished
31A Subfamily
CYP31A1P C. elegans
GenEMBL Z68213
C01F6.3
missing 25 amino acids at C-helix plus in frame stop codon at ERK*
CYP31A2 C. elegans
GenEMBL Z68336 and Z92789
F22B3 and H02I12
This gene is definitely different than CYP31A3, there are 8 amino
acids differences
and the introns are divergent.
CYP31A3 C. elegans
no accession number
T16C6 this sequence revised according to Y17G9.contig 61
cosmid T16C6 lies 3' of E04A4 and 5' of R11E3. Y17G9 contig 61 covers
this
region. E04A4 ends at 29981 of Y17G9 contig 61 R11E3 starts at 49037
of Y17G9
contig 61 this sequence lies between 41545-45037
CYP31A4P C. elegans
no accession number
Pseudogene related to CYP31A sequences 44849-45028 of Y17G9.contig61
C-TERMINAL exon fragment, This sequence is inside the last intron of
CYP31A3
CYP31A5P C. elegans
CYP31B1 Ips paraconfusus (California fivespined ips, bark beetle)
No accession number
Dezene Huber
Submitted to nomenclature committee May 19, 2005
54% to CYP31A2 C. elegans over 456aa
missing 5' end (no signal peptide)
possible nematode contamination, not an insect-like sequence
32A Subfamily
CYP32A1 C. elegans
GenEMBL U53148
C26F1.2
CYP32B1 C. elegans
33A Subfamily
CYP33A1 C. elegans
GenEMBL U55365
C12D5.7
33B Subfamily
CYP33B1 C. elegans
GenEMBL U50311
C25E10.2
33C Subfamily
CYP33C1 Caenorhabditis elegans (nematode worm)
GenEMBL AF039053
C45H4.a near 35k
CYP33C2 Caenorhabditis elegans (nematode worm)
GenEMBL AF039053
C45H4.b near 38k
also on Y10C10 contig 60. Probable end of 33C2 gene is on contig 57,
but the intron ends of these two contigs do not overlap yet.
CYP33C3 Caenorhabditis elegans (nematode worm)
GenEMBL AF016676
F41B5.b 5k to 7k
CYP33C3 Caenorhabditis elegans (nematode worm)
GenEMBL D35162
EST 5 prime read with heme signature
3 prime fragment of this clone yk18a10 = D32508 (not in coding region)
this is a portion of clone YK824
CYP33C4 Caenorhabditis elegans (nematode worm)
GenEMBL AF016438
F44C8 whole gene of EST CEL10E1
CYP33C4 Caenorhabditis elegans
GenEMBL M88882 (501bp)
EST CEL10E1
2nd frame contains PPGP and KKYG from N-terminal region of
many P450s.
CYP33C5 Caenorhabditis elegans (nematode worm)
GenEMBL AF016676
F41B5.c
CYP33C6 Caenorhabditis elegans (nematode worm)
GenEMBL AF016676
F41B5.e
CYP33C7 Caenorhabditis elegans (nematode worm)
GenEMBL AF016676
F41B5.d
CYP33C8 Caenorhabditis elegans (nematode worm)
GenEMBL AF003385
R08F11
CYP33C9 Caenorhabditis elegans (nematode worm)
GenEMBL AF016449
C50H11
CYP33C10P Caenorhabditis elegans (nematode worm)
GenEMBL AF016676
F41B5.a
contains first two exons split by 3700bp and no C-terminal sequence.
CYP33C11 Caenorhabditis elegans (nematode worm)
no accession number
Y49C4 contig 103
CYP32C12P C. elegans
33D Subfamily
CYP33D1 Caenorhabditis elegans (nematode worm)
GenEMBL Z92804
K05D4.4 (previously found on F10A3 and F11A5 at early stages of
sequencing)
CYP33D2X Caenorhabditis elegans (nematode worm)
GenEMBL Z92830
F11A5
this sequence is really the same gene as CYP33D1
CYP33D3 Caenorhabditis elegans (nematode worm)
GenEMBL Z81487 (C54E10), AL021470 (Y17D7A.4) and Z98877 (Y69H2)
GenEMBL AL020988 (Y80D3)
33E Subfamily
CYP33E1 Caenorhabditis elegans (nematode worm)
GenEMBL U61945
C49C8.4
CYP33E2 Caenorhabditis elegans (nematode worm)
GenEMBL U61952
F42A9.5
CYP33E3P Caenorhabditis elegans (nematode worm)
GenEMBL U61952
F42A9.4
missing C-terminal 3' neighbor is C49C8 (with CYP33E1)
34A Subfamily
CYP34A1 Caenorhabditis elegans (nematode worm)
GenEMBL Z81119 and AL022301 (Y75B12)
T10H4.10
There are two P450s listed in this clone as one gene
This is the first one
CYP34A2 Caenorhabditis elegans (nematode worm)
GenEMBL Z81119 and AL022301 (Y75B12)
T10H4.11
There are two P450s listed in this clone as one gene
This is the second one
CYP34A3 Caenorhabditis elegans (nematode worm)
GenEMBL Z81047 and AL022301 (Y75B12)
C41G6.1
CYP34A4 Caenorhabditis elegans (nematode worm)
GenEMBL AF068712
T09H2.1
CYP34A5 Caenorhabditis elegans (nematode worm)
GenEMBL AF039050
B0213.10
CYP34A6 Caenorhabditis elegans (nematode worm)
GenEMBL AF039050
B0213.11
CYP34A7 Caenorhabditis elegans (nematode worm)
GenEMBL AF039050
B0213.12
CYP34A8 Caenorhabditis elegans (nematode worm)
GenEMBL AF039050
B0213.14
CYP34A9 Caenorhabditis elegans (nematode worm)
GenEMBL AF039050
B0213.15
CYP34A10 Caenorhabditis elegans (nematode worm)
GenEMBL AF039050
B0213.16
35A Subfamily
CYP35A1 Caenorhabditis elegans (nematode worm)
GenEMBL U97008
C03G6 near 19k
CYP35A2 Caenorhabditis elegans (nematode worm)
GenEMBL U97008
C03G6 near 22k
CYP35A3 Caenorhabditis elegans (nematode worm)
no accession number
K09D9
CYP35A4 Caenorhabditis elegans (nematode worm)
GenEMBL AF016418
C49G7.8
CYP35A5 Caenorhabditis elegans (nematode worm)
GenEMBL AF039049
K07C6.5 [and part of F40C5 contig 6 9-578 plus strand Note:
newest version of
F40C5 does not contain this P450. probably an error corrected by the
sequencers]
NOTE: K07C6 IS NOW IN GENBANK AND IT CONTAINS 4 P450S
It is part of a contig with T09H2 and B0213 that has 13 P450s in a
cluster
6 P450s on B0213 appear to have inserted themselves into a cluster
of
three olfactory receptor-like genes. K09D9 is the next clone 3' and
it has at least
one more P450. C49G7 is next with one more P450
Contig order K07C6 T09H2 B0213 K09D9 C49G7
35B Subfamily
CYP35B1 Caenorhabditis elegans (nematode worm)
GenEMBL AF039049
K07C6.4, [F40C5 contigs 5 and 14 Note: newest version of F40C5 does
not
contain this P450. probably an error corrected by the sequencers]
CYP35B2 Caenorhabditis elegans (nematode worm)
GenEMBL AF039049
K07C6.3 [and F40C5 contig 15 Note: newest version of F40C5 does not
contain
this P450. probably an error corrected by the sequencers]
CYP35B3 Caenorhabditis elegans (nematode worm)
GenEMBL AF039049
K07C6.2
35C Subfamily
CYP35C1 Caenorhabditis elegans (nematode worm)
GenEMBL Z77652
C06B3.3
35D Subfamily
CYP35D1 Caenorhabditis elegans (nematode worm)
GenEMBL Z83105
F14H3 near 20k
CYP35D2P Caenorhabditis elegans (nematode worm)
GenEMBL Z83105
F14H3 near 14k
only N-terminal is present
36A Subfamily
CYP36A1 Caenorhabditis elegans (nematode worm)
GenEMBL Z83220
C34B7.3
37A Subfamily
CYP37A1 Caenorhabditis elegans (nematode worm)
GenEMBL Z81493 (F01D5) and Z92851 (Y39G8)
37B Subfamily
CYP37B1 Caenorhabditis elegans (nematode worm)
GenEMBL Z93381 and Z93389
F28G4 and T13F3
CYP38A1 Suberite domuncula (sponge)
GenEMBL Y17816 (1789bp)
Mueller,W.E.G., Wiens,M., Batel,R., Steffen,R., Borojevic,R. and
Custodio,M.R.
Establishment of a primary cell culture from a sponge: Primmorphs
from Suberites domuncula
Mar. Ecol. Prog. Ser. In press
most similar to the CYP4 family
MLDFVIFAITAVAGLIGILLFFYFSRSTETKPVSSASPTSTIPR
WSAPPADIEKGDLDVMMKKHGSLHQFLLHLHDNGKTPVTSFWWGKTHVVSFCSPQAFK
ESAVFVNRPVELFVGFEPLITPFSIQYANDEDWVQRSKCLYHTLKGDDLKSYFHHFVQ
IAQEEESLWSSYTSDKEVSLTKEVFPMTIKGIARTCFGDIFKDENELSKMAESYHVCW
RTMEEGVPEAGSKRETEFLKHRRVLEDIIRRIIQERKEGEDLQELPFIDSMLQNYDSE
DKIIADAISFMVGGFHTSGYMFTWMLWYLSSHPESQDRLRTEIERETGGERGDRLKEY
SLRADTFLRQVQDETIRLSTLAPWAARYSDKKVTVCGYTIPAKTPMIHALGVGLKNKT
VWENTDSWDPDRFSPNGRRGNDFCPFGVHSRRKCPGYLFSYFEVGVFASILLSRFEIV
PVEGQTVIQVHGLVTEPKDDIKIYIRSRKED"
CYP39 human
GenEMBL EST R07010 R11279 and UNIGENE entry Hs.25121
covers the C-terminal part of a P450. The 2 ESTs with coding
regions
are not found in UNIGENE, but the opposite end of EST R11279 =
R11221 and it
is in UNIGENE with 13 EST sequences all from the 3 prime noncoding
region.
This sequence is most like CYP4A11, but the percent identity is only
39%. Since this is the most conserved region of P450s, the sequence
must be in a
new family. More sequence is known form the mouse homolog.
ENLLLIKWCVLETIRLKAPGVITRKVVKPVEILNYIIPSGDLLMLSPFWLHRNPKYFPEPELFKPERWEKGKFRRKHSFL
GTASWA
FGAGSSQCPGKVFALLEVQVC
CYP39A1 human
AC008104 AL035670 note heme region exon corrected 1/18/02
MELISPTVIIILGCLALFLLLQRKNLRRPPCIKGWIPWIGVGFEFGKAPLEFIEKARIK
YGPIFTVFAMGNRMTFVTEEEGINVFLKSKKVDFELAVQNIVYRT
ASIPKNVFLALHEKLYIMLKGKMGTVNLHQFTGQLTEELHEQLENLGTHGTMDLNNLVR
HLLYPVTVNMLFNKSLFSTNKKKIKEFHQYFQVYDEDFEYGSQLPECLLR
NWSKSKKWFLELFEKNIPDIKACKSAKDNSM
TLLQATLDIVETETSKENSPNYGLLLLWASLSNAVP
VAFWTLAYVLSHPDIHKAIMEGISSVFGKAG
KDKIKVSEDDLENLLLIKWCVLETIRLKAPGVITRKVVKPVEIL
NYIIPSGDLLMLSPFWLHRNPKYFPEPELFKPERW
KKANLEKHSFLDCFMAFGSGKFQCPARW
FALLEVQMCIILILYKYDCSLLDPLPKQ
SYLHLVGVPQPEGQCRIEYKQRI
CYP39A1 Macaca fasicularis
GenEMBL AB220544.1
CYP39A1 Canis familiaris (dog)
GenEMBL XM_538946.2
CYP39 mouse
GenEMBL ESTs AA096922, AA606237
Note: this sequence has a poorly conserved I Helix
consensus
QFKTYDEGFEYGSQLPEWLLRNWSKSKRWLLALFEKNIGNIKAHGSAGHS
GTLLQAILEVVETETRQYSPNYGLVVLWAALANAPPIAFWTLGYILSHPDIHRTVL
ESISSVFGTAGKDKIKVSEDDLKKLLIIKWCILESVRLRAPGVITRKVVK
PVKILNHTVPSGDLLMLSPFWLHRNPKYFPEPESFKPERWKEANLDKYIF
LDYFMAFGGRKFQCPGKWFALLEIQLCIILVLYKYECSLLDPL
Cyp39 mouse
GenEMBL NM_018887.1 AF237981
39a1 (oxysterol 7alpha-hydroxylase) 72% to human
MGIMELFSPIAIAVLGSCVLFLFSRLKNLLGPPCIQGWIPWIGAGLEFGKAPLEFIEKARIKY
GPVFTIFAMGNRMTFVSEEEGINVLLKSEHVDFESAVQSPVYHTAWIPKNVFSALHERLY
ALMKGKMGTFNTHHFTGPLTEELHEQLEGLGTHGTMDLNDFVRYLLYPATLNTLFKKGLF
LTDKRTIKEFYQQFKTYDEGFEYGSQLPEWLLRNWSKSKRWLLALFEKNIGNIKAHGSA
GHSGTLLQAILEVVETETRQYSPNYGLVVLWAALANAPPIAFWTLGYILSHPDIHRTVL
ESISSVFGTAGKDKIKVSEDDLKKLLIIKWCILESVRLRAPGVITRKVVKPVKILNHTVP
SGDLLMLSPFWLHRNPKYFPEPESFKPERWKEANLDKYIFLDYFMAFGGGKFQCPGRW
FALLEIQLCIILVLYKYECSLLDPLPKQSSRHLVGVPQPAGKCRIEYKQRA*
CYP39A1 Bos taurus (cow)
See cattle page for details
192 MEFISPTVIIILSCVAVLLFLQWKNLRRPPCIRGWIPWIGAGFEFGKTPLEFIEKARIK
YGPVFTVIVMGTRMTFVTEEEGINVFLKSKEINFELAVQNPVYHT
ASIAKNIFLKLHEKLYITVKGKMGIFNLYKFTGQLTEELQEQLQNLGTHGTTDLNKFMR
HLLYPVTVNILFKKGLFPTDERKIREFHQHFQAYDEGFEYGSQLPECLLR
NWSKSKKWLLALFEKNIPDIKTHKSAKENYP
TVMQAVLDLLEMEANEQKSPNYGLLLLWASLSHTVP
VAFWTFAFVLSHPNIHRTIMEGISSVFGTAG
KDKIKVSEDDLKKLPLIKWCILETIRLRAPGVIARKVLKPVKIL
DYTVPSGDLLMLSPFWLHRNPKYFPEPDLFKPERW
KEANLEKHAFLDCFMAFGSGKYQCPGRW
LALLEIQICIILIFYYYDCSLLDPLPKQ
SSLHLVGVQQPEGRCRIQFKQRK*
CYP39A1 Sus scrofa (miniature pig)
no accession number
Haitao Shang
Submitted to nomenclature committee May 23, 2007
partial sequence
80% to human CYP39A1, complete seq constructed from ESTs
Ortholog of human CYP39A1
CYP39A1 pig
ESTs BP441358.1, BP458693.1, CV872464.1, DN131248.1, CB287479.1
DN131598.1, CN156146.1, DN115951.1, BP166812.1, DN116306.1, CN154029.1,
MEFISPAVIIILGCVTLLLFLQWKNLRGPPCIGGWIPWIGAGFEFGKAPLEFIEKARIKY
GPIFTVLAMGTRMTFVTEEEGMNVFLKSKEVNFELAVQNPVYRTASIPKNIFLKLHEKLC
VMMKGKMGTFNLYQFTGQLTEELHKQLENLGTHGTMDLNNLVRHLLYPVTVNVLFK
KGLFPTNERKIREFHQHFQAYDEGFEYGSQ LPERLLRNWSKSKNWLLALFEKNI PDIKTY
KSAKDHSQTIMQDILDLLEMETNEQMSPNYGLLLLWASLSNAVPVTFWTLAFILSHPNIH
RTILEGISSVFGTTGKDKI
EVSEDNLKKLPLIQWCILETIRLRAPGVITRKVLKP
VKILNYTVPSGDLLMLSPFWLHRNPKYFPEPDLFKPERWKKANLEKHAFLDWFMAFGTGKYQCP
GRWLALLEIQMCVILIFYYYDCTLLDPLPKQSSLHLVGVQQPEGQCRVEFKQRK*
CYP39A1 Gallus gallus (chicken)
GenEMBL BG712800 BG710422 BU226060 AI979980 BU247492 BU226060
XM_420064.1
57% TO 39A1 HUMAN
MAAAVLLAALLAVAVVAWVLFPPSEP
RRPPCIGSRVPCLGAAF
RLARAPLEFIERARAEHGPIFTVFALGKRFTFVTEDEGAEVFFTSKDLNFEQAVQRVV
ANAVSVPAEAFYQNHGRLYSMMKGKMGPSNLHMFTGTLCKELHEHMAHLGTAGTGDLMEL
VRHIMYTAVVNTLFGKGACPTSHSELREFEEHFWKYDEDFEYASQMPECFLRNWSKSKKW
LLKLFEKVVSDAERTNPSETTSKTLLQHLLDNLQGK
HLAPNYGLLMLWASQANAVPIAFWTLVFILSSP
SVYKKVMEDLTSVFGNAGKDEIEVSEEDLKNLPYIKWCTLEAIRLRSPGAITKKVIKPIR
IQSFTIPAGDMLMLSPYWLHRNPKYFPDPEMFKPDRWKEANLEKNAFLDGFVAFGGG
NHQCPGRWFAIMEIQLLVALFLYKYEFVLLDAVPKESPLHLVGTQQPLTPFRVQYKGWE*
CYP39A1 Xenopus tropicalis
See Xenopus pages for seq
CYP39A1 Xenopus laevis
GenEMBL BC106433.1
CYP39A1 Danio rerio (zebrafish)
GenEMBL NM_001030189.1
CYP39A1 Salmo salar (Atlantic salmon)
GenEMBL DW563555.1 CB512602.1 ESTs
EVVTLVLSLVILAISAHLLFAGNYPNAPPCIKGWIPWFGVAFEFGKSPLTFISQARDKYGPVFT 193
194 VVAAGKRLTFVTLHEDFRTFFMSKDVDFEQAVQEPVHNTASISKDSFYKFHPACNTLIKG 373
374 RLTPGNVAQLTDHLCEEFNDHLET
1 LGDQGSGGLSELVRAVMYPAVMSNLLGKYNSPGSPFTMEQFKEKFAIYDEGFEYGSQLPD 180
181 MFLREWASSKCWLLSLLGNMVVKAEDDETSSESGNRTLLQHLATLITDKFLPNYGLL 351
352 MLWASLANAIPITFWAVAFILSNPTVYQTAMEQINAALKDQDTRKTKVTAEELQQMPYVK 531
532 WCILEAIRLRAPGAITRRVVRPLRIQNYIIPPGDLLMVSPYWAHRNPHYFPEPEEFKPER 711
712 WEKADLVKN 738
CYP39A1 Oncorhynchus mykiss (trout)
GenEMBL BX298944.3 cDNA
25 LVLFLVVLAISAHLLFGGNYPNAPPCIKGWIPWFGVAFEFGKSPLTFISQARDKYGPVFT 204
205 VVAAGKRLTFVTLHEDFRTFFMSKDVDFEQAVQEPVHNTASISKESFYKFHPACNTLIKG 384
385 RLTPGNVAQLTDHLCEEFNDHLETLGDQGSGGLNELVRAVMYPAVMSNLLGKYNSPGSPF 564
565 TMEQFREKFAIYDEGFEYGSQLPDXVLRXWASSKCWLLSLLGNMXCQAEDDETSSESGNR 744
745 TFTAHLV 765
40A Subfamily
CYP40X rat, mouse, human (name changed to CYP27B1)
Note: This family was created based on the rat sequence AF000139 submitted to
the Nomenclature Committee in May 1997. This was the only sequence available at
the time and it was about 38% identical to rat CYP27. The sequence was
considered a new family and named CYP40. Since then, there have been additional
sequences determined for the rat mouse and human homologs. The rat sequence
AB001992 differs from the AF000139 sequence at 34 amino acids, mostly in two
regions that are probably frameshifts. The sequence AB001992 is 42.3% identical
to the rat CYP27A sequence. The mouse and human sequences are also more than 40%
identical to the CYP27A sequence. Therefore, they belong in the CYP27 family as
a new subfamily CYP27B1. Since there only appears to be one gene in each
species, all three species orthologs will be called CYP27B1. CYP40 has been
retired and this is indicated by the X in CYP40X.
41A Subfamily
CYP41 Boophilus microplus (southern cattle tick)
GenEMBL U92732 (414bp)
Crampton,A.L., Miller,C., Baxter,G.D. and Barker,S.C.
Expressed sequenced tags and new genes from the cattle tick,
Boophilus microplus.
Exp. Appl. Acarol. 22 (3), 177-186 (1998)
EST is from the middle of the P450
whole sequence known but still confidential.
42A Subfamily
CYP42 C. elegans
GenEMBL AL020988 (Y80D3), M89401 (EST cm08B12)
43A Subfamily
CYP43 C. elegans
GenEMBL AF026203
E03E2.1
44A Subfamily
CYP44A1 C. elegans
GenEMBL U21321
CELZK177
only mitochodrial-like P450 in C. elegans
MRRSIRNLAENVEKCPYSPTSSPNTPPRTFSEIPGPREIPVIGN
IGYFKYAVKSDAKTIENYNQHLEEMYKKYGKIVKENLGFGRKYVVHIFDPADVQTVLA
ADGKTPFIVPLQETTQKYREMKGMNPGLGNLNGPEWYRLRSSVQHAMMRPQSVQTYLP
FSQIVSNDLVCHVADQQKRFGLVDMQKVAGRWSLESAGQILFEKSLGSLGNRSEWADG
LIELNKKIFQLSAKMRLGLPIFRLFSTPSWRKMVDLEDQFYSEVDRLMDDALDKLKVN
DSDSKDMRFASYLINRKELNRRDVKVILLSMFSDGLSTTAPMLIYNLYNLATHPEALK
EIQKEIKEDPASSKLTFLRACIKETFRMFPIGTEVSRVTQKNLILSGYEVPAGTAVDI
NTNVLMRHEVLFSDSPREFKPQRWLEKSKEVHPFAYLPFGFGPRMCAGRRFAEQDLLT
SLAKLCGNYDIRHRGDPITQIYETLLLPRGDCTFEFKKL
CYP44A1 Caenorhabditis briggsae
XM_001679715.1
85% to CYP44A1 ortholog
MRRSIKILAENLENCPYTSATTTAPPRRFSEIPGPREIPVIGNS
GNLKYAIGTDAETIENYNKHLEEMYNKYGKIVKENLGFGRKYVVHVFDPADAQTVFAA
DGKTPFIVPLQETTQKYREMKGMNPGLGNLNGPEWYRLRSSIQHAMMRPQSVQTYLPF
SQKVSDDLVRHVAEEQIRFGNANMQKVAGRWSLESAGQILFEKSLESLGNRSEWADGL
IELNKKIFQLSAKMRLGLPIFRLFSTPSWRKMVKLEDEFYAEVDRLMDDALDNLKISE
SDSQNMRFASYLINRKELNRRDVKVILLSMFSDGLSTTAPMLIYNLYNIATHPEALRK
IQEEIKEDPTSSKLPYLRACIKETFRMFPIGTEVSRITQKDLTLSGYLIPAGTAVDIN
TNILMRNMVLFSDSPHEFKPERWLEKSKSVHPFAFLPFGFGPMCAGRRFAEQDLLTSL
AKLCANFDIRHRGEPITQIYETLLLPRGNCEFEFKKL
CYP44A1 Caenorhabditis brenneri strain PB2801
ABEG01003567.1
85% to CYP44A1 ortholog
10467 MRRSIKKLAEHVEKCPFNGNPTKSSVTRRFSEIPGPREIPVFGNAGNFKYAIGS (1) 10628
10675 DAKTIESYNIHLEEMYRKYGKIVKENLGFGRKYVVHVFDP (1) 10794
10859 ADAQTVLAADGKTPFIVPLQETTQKYREMKGMNPGLGNL (2) 10975
11023 NGPEWYRLRSSVQHAMMRPQSVQT (2) 11094
11141 YLPFSQKVSDDLISHVAKVQLRFGH 11215
11216 VNMQKVAGRWSLESAGQILFEKSLGSLGDRCEWADSLIELNKKIFQLSAK (2) 11365
11413 MRLGLPIFRLFSTPSWRKMVELEDQFYAEVDRLMDNALDKLKVNDSDNQNMRFASYLIN 11589
11590 RKELNRRDVKVILLSMFSDGLST (0) 11661
11703 TAPMLIYNLYNIAAHPEAQREIRKEIQDDPSSSKLPFLRACIKETFRMFPIGTEVSRITQ 11882
11883 KDLILSGFDVPAGTAVDINTNILMR (2) 11957
12004 DEVLFSDSPRIFKPQRWLEKSKEVHPFAFLPFGFGPRMCAGRRFAEQDLLTSLAK 12168
12169 LCANFEICHRGEPITQIYETLLLPRGNCEFEFRKL* 12276
CYP44A1 Caenorhabditis remanei strain PB4641
AAGD02002953.1
83% to CYP44A1 ortholog
11277 MRKSLQKFAEKCPFSATSVKTSVPRRFSEIPGPLELPIIGNIGNFKYAVRS (1) 11429
11477 DSKTIEGYNHHLEEMYHKYGKIVKENLGFGRKHVIHLFDP (1)
ADAQIVLAADGKTPFIVPLQETTQKYREMKGMNPGLGNL (2) 11761
11802 NGPEWYRLRSSIQHAMMRPQSVQT (2)
YLPFSQIVSEELVKHVAKEQVRFGH 11990
11991 VNMQKVAGRWSLESAGQILFEKSLGSLGDRSEWADGLIELNKKIFQLSAK (2) 12140
12187 MRLGFPLFRLFSTPSWKKMVELEDRFYAEVDRLMDDALDKLTVKDSD ()
12379 SQNMRFASYLINQKELNRRDVKVILLSMFSDGLST (0) 12483
12530 TAPMLIYNLYNIAAHPDAQHKIQKEIKEDPTSTKLPFLRSCIKETFRMFPIGTEVSRITQ 12709
12710 KDLILSGFHVPSGTAVDINTNILMR (2) 12784
12829 NEVLFSDSPHEFKPQRWLEKSKDVHPFTFLPFGFGPRMCAGRRFAEQDLLTSLAK 12993
12994 LCANFDIHHRGEPITQIYETLLLPRGNCEFEFRKL 13098
CYP44A1 Caenorhabditis japonica
chrUn:100501692-100508750
From UCSC browser
MRTSLRKFAENVQKCPFPEKSSDIPARRFSEIPGPREVPFFGSTENLKFAIKS (1)
DAQTIENYNEHLAEMYKNYGKIVKENLGFGRKCVVHLFDP (1)
VDAQTVFAADGKTPFIVPLQETTQKYREMKGMNPGLGNL (2)
NGPEWYRLRSSIQHAMMRPQSVQT (2)
YLPFSQKVSQDLVSHVASEQARFGAVNMQKVAGRWSLESAGQILFEKSL
GSLAENSEWADGLIELNKRIFQLSAK (2)
MRLGLPIFRFFPTPSWRKMVQLEDEFYAEVDRLMDDALDKLKVLDIVEN (2)
SKNLRFASYLINRKELNRRDVKVILLSMFTDGLST (0)
TAPMLIYNLFNIATHPVAQERIRKELEK (1)
DTTKLPFLRACIKETFRMFPIGTEVSRITQKDLVLSGFEIPAGTPVDINTNVLMR (2)
NTVLFADDPLIFKPERWLDKSAATHHPFAFLPFGFGPRMCAGRRFAEQDLLTSLAKLCAKFEIRHVGEPVTQIYETLLLPRGNCKFEFASV*
CYP44A1 Ascaris suum
CB101937.1
44% to CYP44A1 aa 134-170 possible ortholog
NGDEWYRLRSSVQKAMMRPQAVRQYLPAVNRVADELLQFI
CYP44B1 Lottia gigantea (owl limpet)
JGI Protein ID:163695
74% to CYP44B2, 34% to CYP44A1 C. briggsae
35% to CYP44A1 C. remanei
MWGRNVKHAANY
LKRCSISTSLGISRSEGRAVNSNDVSDYNQALPFEDIPGPRG
IKYLGTLHHYMTGRLDKYKYQD
ILEEYFSKYGPIFKETLF
GSTIVHVCDPKMFEVIYQDEGKYPEIEPLVEPSQKFR
GEGDLSLGLGNSNGEKWYNLRAIVQKSML
KPAEVSSHLQIINEIANDFVDKTLHKGYS
DDFLLDTSIWKLESGGALSFGKRPGYLECGSA
QEMEAMKLVKMVDNTFTLATDLKFSFPLYKYIKTPAYKA
LLDSEQYINDVTSVYLSETLKHIETEL
ERTDDFNDIQNTYRFLYSLLGHKN
LSKKDMEVIVLSMFIDGLRTVVPVLISVLFCLSQS
PRVQEKLFTEIYNVLEDRKYIAASDIAQLPYLKACVK
ETFRLYPIGLEISRINQNDMVLGEYKIPSG
TKLNLNNFVLNRD
PNIFANPEAFVPERWLRGSEHAVDA
HPYLLLPFGRGPRMCVGRRFAEQDLYVLISK
LIQNCEPHWTGEKYEHQFKI
LVYPACPVKFQFKPRQSSSK
CYP44B2 Lottia gigantea (owl limpet)
JGI Protein ID:163698
74% to CYP44B1, 37% to 44A1 C. briggsae
MWRNFQKNFFSVCGKVNR
IKRCWISTSLGISRSEGRAVNSNDVSDYNQALPFEDIPGPQG
IKYLGTLPHYMTGRLDKYKYQD
ILEEYFSKYGPIFKETLF
DKTVVHVCHPDMIKVVYQDEGKYPEIEPLMEPTQKSR
CDEGMSLGLGNTNDEQWYRLRSAVQKHMM
RPGEVSNYLPQVNEVANEFVDKTMRKGYS
DDFILDASIWSLESSGYISFGKRLGYMEGGSE
KEAEGVKVVKKNDEAFTLATDLKFSLPFYQQLQTPKYKAWHEAE
NYTTKVASVYFENVLKFLETHL
DSSSEPKKVQNTYRFLYSLLGHKN
LSKKDMEVIVLSMFIDGLRTVVPVLISVLFCLSQS
PRVQEKLFTEIYNVLEDRKYIAASDIAQMPYLKACVK
ETFRLHPNALEISRVTKNNMVLGGYQIPSG
SKLNINAFNMYRN
SDIFENPQNYCPERWLRGWEHAVDV
HPYLLLPFGRGPRMCVGRRFAEQDLYVLISK
FIQNCEPQWRGSEFHQQYKI
LMYPDRPVKFEFKSR
CYP44C1 Capitella capitata (Gallery worm, polychaete worm, annelid)
estExt_Genewise1Plus.C_850046 [Capca1:171547]
39% to CYP44B1, 37% to 44A1 C. briggsae
MATRSLLQQQPSARVKPFSLIPGPRGLPYLGTLLDYKTGKYAPSSFDKAFRANHQKYGKIFKETIAGSTK
VHLCDPDYAQRLFQLEGKRPHTPPLLETSKMYRKINRLSLGLGNMNGEKWSRSRKAVGHLLMKPKSVSQF
LPHINGCVDDFITKIHHLRDEEGLVENFSNEIKMWTLESTANICFETRLGALNGTAAQSDFIQRMVNAMD
SMLEQALKLRFSFGWWRLLPTPAWQKLYDCEDFFFSNAQRIVNEAISKMDALLESGDFVEGHSYPFLSYL
LGREELSLEDVRIISMAMFSDGMITTSPTVASQLYCLATNPEVQDKVYEEVLRVVGIKTKEITSGHLEEL
SYLKSCIKEGFRFFPIGTEISRIVPTDITIGGYLIPKGTHVEVNTNMLLQSPQYFHDAHRFIPERWTRDG
SASNVHPYLVRPFSCGPRMCPGKRIAEQEMLTFIAKLLRHYRVEWRESHEMSQRYRILLAPDCSADFYFI
PRNN*
45A Subfamily
CYP45 Homarus americanus (American lobster)
GenEMBL AF065892 (1581bp)
Snyder,M.J.
Identification of a new cytochrome P450 family, CYP45, from the
lobster, Homarus americanus, and expression following hormone and
xenobiotic exposures
Arch. Biochem. Biophys. (1998) In press
46A Subfamily
CYP46 human
GenEMBL NM_006668
Lund EG, Guileyardo JM and Russell DW.
cDNA cloning of cholesterol 24-hydroxylase, a mediator of
cholesterol homeostasis in the brain.
Proc. Natl. Acad. Sci. U.S.A. 96, 7238-7243 (1999)
32% identity with Drosophila 4D2
ESTs H06539, H51951, R36281
mouse homolog EST AA096922
MSPGLLLLGSAVLLAFGLCCTFVHRARSRYEHIPGPPRPS
FLLGHLPCFWKKDEVGGRVLQDVFLDW
AKKYGPVVRVNVFHKTSVIVTSPESVK
KFLMSTKYNKDSKMYRALQTVFGER
LFGQGLVSECNYERWHKQRRVIDLAFSRSSLVSLMETFNEKAEQLVEILEAKADGQTPVSMQDMLTYTAMDILAK
AAFGMETSMLLGAQKPLSQAVKLMLEGITASRNTLAK
FLPGKRKQLREVRESIRFLRQVGRDWVQRRREALKRGEEVPADILTQILK
AEEGAQDDEGLLDNFVTFFIA
GHETSANHLAFTVMELSRQPEIVAR
LQAEVDEVIGSKRYLDFEDLGRLQYLSQ
VLKESLRLYPPAWGTFRLLEEETLIDGVRVPGNTPLL
FSTYVMGRMDTYFEDPLTFNPDRFGPGAPK
PRFTYFPFSLGHRSCIGQQFAQ
MEVKVVMAKLLQRLEFRLVPGQRFGLQEQATLKPLDPVLCTLRPRGWQPAPPPPPC
Cyp46 mouse
GenEMBL AF094479 NM_010010 ESTs AA096922, R75217
no UNIGENE entry
MSPGLLLLGSAVLLAFGLCCTFVHRARSRYEHIPGPPRPSFLLG
HLPYFWKKDEDCGRVLQDVFLDWAKKYGPVVRVNVFYKTSVIVTSPESVKKFLMSTKY
NKDSKMYRALQTVFGERLFGQGLVSECDYGRWYKQRKVMDLAFSRSSLVSLMETFNEK
AEQLVEILEAKADGQTPVSMQDMLTCATIDILAKAAFGMETSMLLGAQKPLSQAVKVM
LEGISASRNTLAKFMPGKRKQLREIRESIRLLRQVGKDWVQRRREALKRGEDMPADIL
TQILKAEEGAQDDEVLLDNFVTFFIAGHETSANHLAFTVMELSRQPEIVARLQAEVDE
VVGSKRHLDYEDLGRLQYLSQVLKESLRLYPPAWGTFRLLEEETLIDGVRVPGNTPLL
FSTYVMGRMDTYFEDPLTFNPDRFGPGAPKPRFTYFPFSLGHRSCIGQQFAQMEVKVV
MAKLLQRIEFRLVPGQRFGLQEQATLKPLDPVLCTLRPRGWQPAPPPPPC
CYP46A1 Bos taurus (cow)
See cattle page for details
MSPGLLMLLGSAVLVVFGLCCTFVHRARSRYEHIPGPPRPS (2)
FLLGHLPYFWKKDEVCGRVLQDVFLDW (2)
AKKYGPVVRVNVFHKTSVIVTSPESVK (0)
KFLMSTKYNKDSKMYHAIQTVFGER (1)
LFGQGLVSECXYERWHKQRRIMDLAFSRSSLVGLMGTFNEKAEQLVEILEAQ 198
199 ADGQTPVSMQDMLTCATMDILAKAAFGMETSMLLGAQKPLSRKVKLILEGISASRNTLAK 378
379 FMPGKWKQLXETRESVRFLRQVGKEWVXRXRXALQRGEDVPADILTQILKAEEGAQD
367 DEILLDNFVTFFIAGHETSANHLAFTVMELSRQPEILARLQAEVDEVIGSKRHLDCEDLG 188
187 RLQYLSQVLKESLR 146
LYPPAWGTFRLLEEETLIDGVRVPGNTPLL
FSTYVMGRMDTYFEDPLTNPDRFGPGAPK
PKFTYFPFSLGPRSCIGQQFAQ
MEVKVVMAKLLQRLEFRLVPGQRFGLQEQATLKPLDPVLCTLQPRGWQPAPPPPPC
CYP46A1 pig
BI359892.1, BF193580.1, BF190788.1,
lower case = cow seq.
mspgllmllgsavlvvfglcctfvhrar
SRYEHIPGPPRPSFLLGHLPYFWKK
KDEVCGRVLQDVFLDWAKKYGPVVRVNVFHKTSVIVTSPESVKRFLMSTKYNKDS
KMYHAIQTVFGERLFGQGLVSECDYERWHKQRRVMDLAFSRSSLVSLMGIFNEKAEQLVE
ILEAQADGQTPVSMQDMLTCTTMDILAKAAFGIETSMLLGAQKPLSRKVKLIL
egisasrntlakfmpgkwkqlxetresvrflrqv
GKDWVQRRREALQRGEDVPADILTQILKAEEGAQD
DEILLDNFVTFFIAGHETSANHLAFTVMELSRQPEILARLQAEVDEVIGSKRHLDCEDLG
RLQYLSQVLKESLRLYPPAWGTFRLLEEETLIDGVRVPGNTPLLFSTYVMGRMDTYFEDP
LTFNPDRFSPKAPKPKFTYFPFSLGPRSCIGQQFAQMEVKVVMAKLLQRLEFRLVPGQSFGLQE
Qatlkpldpvlctlqprgwqpapppppc
CYP46A1 Xenopus tropicalis
See Xenopus pages for seq
CYP46A1 Danio rerio (zebrafish)
Seq zebrafish pages for seq
CYP46 Fugu rubripes (pufferfish)
No accession number
Scaffold_4537
60% to 46 human
MGVFNLIFGWISQASIFLLLLLFIALLGYCMYIKYTHMKYDHIPGPPRDS (2)
FFSGHSSKLLDIMKDDGVVHDMFLKW (2)
AETYGPVYKIYFLHHVMVFVSCPETTK (0)
EMLMSPKYTKDKFLHNRIGSLFGQR (2)
FLGNGLVTVRDHEKWYKQRRIMDPAFSSL (2)
YLRSLMGNFNETADKLMDKLSEIADNKTTANMLHLVNCVTMEVLAK (0)
VAFGVDLDLLRKSSPFPRAVELCLKGMVFSIRDTFFM (0)
LNPKNWSFIREVRGACRLLRQTGAQWIQQRKTAMRNGEVPKDILTQIIKSAGK (1)
EEIMTQEDEEFMLDNFLTFFIA (1)
GQETTANQLGFCIMELGRHPDILER (2)
VKKEVDEAIGMKQDISYDDLGHLGYLSQ (0)
VLKETLRLYPTAPGTSRDLKEDMVIGGVHVPGGVVCV (0)
FSSYGMGRMETFFKDPLKFDPDRFDPDAPK (2)
PYYCYFPFSLGPRSCLGQNFAQ (0)
MEAKVVMAKLIQRFDFTLLPGQSFDILDNGTLRPKSGVLCSLRHRDHKK*
CYP46A2P Fugu rubripes (pufferfish)
CYP46A2 Danio rerio (zebrafish)
Seq zebrafish pages for seq
CYP46A3P Fugu rubripes (pufferfish)
46A-se1[12:13:14] human
NT_004424.11|Hs1_4581 chromosome 1 CYP46 pseudogene fragment
Old name = CYP46A4P
2405597 DPLTFNPYRFGPGAPKPRFTYFPFSLGHHSCIGQQFAQMEVKVVMAKLLQRLEFQLVPGP 2405776
2405777 RFGLQ*QATLKPLDPELCTLRPRGWQPAAPPPRC 2405878
Cyp47a1x Drosophila melanogaster
GenEMBL AC005556, AC004516, AC004426 intron exon boundaries
approximate
renamed Cyp4aa1 following CYP4Z1
CYP48A1 Trichogramma cacœciae (a parasitoid wasp)
GenEMBL AF207950
BBRC 268, 677-682 (2000) Cloning and expression of cytochrome
P450 genes belonging to the CYP4 family and to a novel
family, CYP48, in two hymenopteran insects, Trichogramma
cacœciae and Apis mellifera by S. Tares, J.B. Bergé and M. Amichot.
123 amino acid sequence from I-helix to PERF region
45% identical to CYP47A1, but sure to drop into the
mid to low 30% range for a full length sequence
clone name 16Tc
66% to CYP4AB22
TAAAMTFTIMLLAENEEAQDKARAEVTQIFDRCDGKIEMQDIQD
MTYLEWCVKEALRLYPPVSTMTRTMSEDLQLSKDFLVPAGAEVIFHLYDTHRDPNFWE
DPDKFDPDRFSPERSHGRHPFSYL
CYP48A2v1 Trichogramma cacœciae (a parasitoid wasp)
GenEMBL AF207951
BBRC 268, 677-682 (2000) Cloning and expression of cytochrome
P450 genes belonging to the CYP4 family and to a novel
family, CYP48, in two hymenopteran insects, Trichogramma
cacœciae and Apis mellifera by S. Tares, J.B. Bergé and M. Amichot.
124 amino acid sequence from I-helix to PERF region
40% identical to CYP47A1, but sure to drop into the
mid to low 30% range for a full length sequence
clone name 15Tc
72% to CYP4AB10
TGMAMTFTLMLLAENKEAQTKARAEVTQVMEKYNGNLNTSSLQE
LPYLERCIKESLRLFPPVATLLRYTADELQLKNALVPADSHIMIHLYDTHRDANYWPN
PDVFDPDRFLPERSANRHTFAYV
CYP48A2v2 Trichogramma cacœciae (a parasitoid wasp)
GenEMBL AF207952
BBRC 268, 677-682 (2000) Cloning and expression of cytochrome
P450 genes belonging to the CYP4 family and to a novel
family, CYP48, in two hymenopteran insects, Trichogramma
cacœciae and Apis mellifera by S. Tares, J.B. Bergé and M. Amichot.
124 amino acid sequence from I-helix to PERF region
40% identical to CYP47A1, but sure to drop into the
mid to low 30% range for a full length sequence.
58% identical to CYP48A1
clone name 21Tc ony two amino acid differences with CYP48A2v1
71% to CYP4AB10
TGMAMTFTLMLLAENKEAQTKARAEVTQVMEKYNGNLNTSSLQE
LPYLERCIKESLRLFPPVATFLRYTADVLQLKNALVPADSHIMIHLYDTHRDANYWPN
PDVFDPDRFLPERSANRHTFAYV
CYP49A1 Drosophila melanogaster
GenEMBL AC017930 48452-54739 AC007398 AC007418
ESTs AA941795 AI258819 AA697999
mitochondrial P450 fits in the mitochondrial clan
Cyp49a1 Drosophila pseudoobscura
See D. pseudoobscura page on this server for sequence
Obtained from UC Santa Cruz Browser
CYP49A1 Anopheles gambiae (malaria mosquito)
GenEMBL
Submitted by Christelle Abgrall, Hilary Ranson and Rene Feyereisen
See the paper: Evolution of supergene families associated with
insecticide resistance.
Ranson, H., Claudianos, C., Ortelli, F., Abgrall, C., Hemingway, J.
Sharakhova, M.V., Unger, M.F., Collins, F.H. and Feyereisen, R.
Science 298, 179-181 (2002)
Anopheles map name CYPs2l1
CYP49A1 Apis mellifera (honeybee)
WGS section genbank
Submitted by May Berenbaum 2/11/04
AmGroupUn.423a
45% to 49A1 Drosophila. Note this sequence is
named in the same subfamily as other CYP49s since
there seems to be only one orthologous sequence per
species and it does not make sense to name orthologs with
different subfamily names.
CYP49A1 Bombyx mori (silkworm)
BAAB01133495.1 BAAB01150577.1 BAAB01091102.1 BAAB01198981.1
BAAB01133297.1 BAAB01093561.1
44% to CYP301A1
CYP49 and CYP301 are nearly subfamilies of a single family
See silkworm page for sequence
CYP49A1 Tribolium castaneum (red flour beetle)
GenEMBL XP_970738
63% to 49A1 Drosophila, 47% to CYP301A1 Tribolium
MSLSRKLLVPKRALAVAQRAYSTDRPYSTAIMPELLDELAIPEHVERVEATPRPYSAIPGPKELPLIGNA
WRFAPIIGQYKIQELDKVMWSLNRDYGRIVKVGGLIGHPDLLFVFNGDDIEKVFRMEEAMPHRPSMPSLH
YYKQILKKDFFDGNAGVIGVHGPKWEEFRKKVQHALLPPQIAKKYIEPLDVIAGDFLHRMEDMLDENQEL
PNHFLSEIYKWALESVARVSLDTRLGCLEPNLSQNSESQRIINSINTFFWNVAEVELKMPVWRVYKNRSF
KKYIGALEDFRTLCLKHIHKSMEKMQEKNFDEIKEENISIVERILLKTDNPKLAAVLALDLLLVGVDTTS
IAAASTIYQLSQNPEKQQKLFDELQQVLPENDSKIDVSIQDKMPYLKACIKETLRMYPVIIGNGRSLQTD
TVIAGYKVPKGTHVIFPHLVVSNSEDYFHEPHRFLPERWLKTENACPMHKKIHPFVTLPFGYGRRSCLGR
RFAEAELQILLAKIFRKYKVEYNYGPLSYKITPTYVPEQPLKFKLVKRE
CYP49A1 Aedes aegypti (yellow fever mosquito)
CYP49A1 Culex pipiens
CYP49A1 Pediculus humanus humanus (body louse)
No accession number
Reed Johnson and Barry Pittendrigh
Submitted to nomenclature committee March 14, 2007
Ph49, 58% to CYP49A1 Aedes, only 44% to CYP303A1 Aedes,
58% to 49A1 Anopheles gambiae
Pediculus genome site
CYP49A1? Helicoverpa armigera (cotton bollworm)
No accession number
Heiko Vogel
submitted to nomenclature committee Feb. 6, 2008
Contig_262 only 26% to CYP49A1 unidentified mito clan N-term
All best hits are CYP49A1 and this protein is not well conserved
CYP49A2 Bombyx mori (silkworm)
BAAB01014940.1 BAAB01083735.1 BAAB01056249.1
51% to CYP49A1 Drosoph.
CYP49 and CYP301 are nearly subfamilies of a single family
See silkworm page for sequence
Note this is the last two digit number for animal P450s (except CYP51). The
animal P450s will continue at CYP301A1. This leaves 200 possible animal
families before reaching CYP501 and higher that are reserved for lower
eukayotes.
51A Subfamily
A note on nomenclature. CYP51s were originally all called CYP51, because only one
gene was found per species and they all seemed to be in this one conserved family.
However, rice had many CYP51s in at least two sequence groups, so subfamilies
have been designated for CYP51s. These are not the typical subfamilies, but only
one subfamily is created for each major taxonomic group. CYP51A for animals,
CYP51B for bacteria. CYP51C for Chromista, CYP51D for Dictyostelium, CYP51E
for Euglenozoa, CYP51F for fungi. Those groups with only one CYP51 per species
are all called by one name: CYP51A1 is for all animal CYP51s since they are
orthologous. The same is true for CYP51B, C, D, E and F. CYP51G (green plants)
and CYP51Hs (monocots only so far) have individual sequence numbers.
CYP51A1 human
GenEMBL U23942 (3172bp)
Stromstedt,M., Rozman,D. and Waterman,M.R.(1995)
The ubiquitously expressed human CYP51 encodes lanosterol 14 alpha
demethylase, a cyochrome P450 whose expression is regulated by
oxysterols.
Arch. Biochem. Biophys. 329, 73-81 (1996)
unpublished
CYP51A1 human
GenEMBL U51684 to U51692 (genomic sequence)
Rozman,D., Stromstedt,M., Tsui,L.-C., Scherer,S.W. and
Waterman,M.R.
Structure and mapping of the human lanosterol 14-alpha demethylase
gene (CYP51) encoding the cytochrome P450 involved in cholesterol
biosynthesis; comparison of exon/intron organization with other
mammalian and fungal CYP genes.
Genomics (1996) In press
CYP51A1 human
GenEMBL D55653 (3085bp)
Aoyama,Y., Funae,Y, Noshiro,M., Horiuchi,T. and Yoshida,Y.
Occurrence of a P450 showing high homology to yeast lanosterol 14-
demethylase (P450DM) in rat liver.
Biochem. Biophys. Res. Commun. 201, 1320-1326 (1994)
CYP51A1 Macaca fasicularis (cynomolgus monkey)
No accession number
Yasuhiro Uno
Submitted to nomenclature committee 1/11/2005
Clone name mfCYP51A1_7H11
98% to CYP51 human
CYP51A1 Bos taurus (cow)
See cattle page for details
22188 MLDLLQAGGSVLGQAMEQVTGGNLASMLLIACAFTLSLVYLFRLAVGHLAPPLPTGA (0) 22018
20194 KSPPYIVSPIPFLGHAIAFGKSPIEFLEDAYEK (0) 20096
17795 YGPVFSFTMVGKTFTYLLGSEAAALLFNSKNEDLNAEEVYSRLTTPVFGKGVAYDVPNT 17619
16395 VFLEQKKMLKSGLNIAHFRQHVSIIEKETKEYFKSWGESGEK 16270
14644 NLFEALSELIILTASHCLHGKEIRSQLNEKVAQLYADLDGGFSHAAWLLPGWLPLPSFR 14468
13586 RRDRAHREIKNIFYKAIQKRRESGEKIDDILQTLLESTYK 13467
13076 DGRPLTDDEVAGMLIGLLLAGQHTSSTTSAWMGFFLARDKTLQEKCFLEQKTVCGENLPPLTYDQ (0) 12882
11489 LKDLNLLDRCIKETLRLRPPIMTMMRLAKTP 11397
10597 QTVAGYTIPPGHQVCVSPTVNQRLKDSWVERLDFNPDRYLEDSPASGEKFAYVPFGA (1?) 10427
7020 GRHRCIGENFAYVQIKTIWSTMLRLYEFDLIDGYFPTVNYTTMIHTPEKPIIRYKRRSK* 6841
CYP51A1 pig
AB042982, AB009988, NM_214432
MVLLGLLQAGGSVLGQAMEQVTGVNLLSSLLLACAFTLILVYLF
RQAIGHLAPLPAGAKSPPYIFSPIPFLGHAIAFGKSPIEFLENAYEKYGPVFSFTMVG
KTFTYLLGSDAAALLFNSKNEDLNAEDVYSRLTTPVFGKGVAYDVPNPVFLEQKKMLK
SGLNIAHFRQHVSIIEKETKEYFQSWGESGERNLFEALSELIILTASHCLHGKEIRSQ
LNEKVAQLYADLDGGFSHAAWLLPGWLPLPSFRRRDRAHREIKNIFYKAIQKRRQSEE
KIDDILQTLLDSTYKDGRPLTDDEVAGMLIGLLLAGQHTSSTTSAWMGFFLARDKTLQ
EKCYLEQKTVCGEDLPPLTYDQLKDLNLLDRCIKETLRLRPPIMTMMRMAKTPQTVAG
YTIPPGHQVCVSPTVNQRLKDSWVERLDFNPDRYLQDNPASGEKFAYVPFGAGRHRCI
GENFAYVQIKTIWSTMLRLYEFDLIDGYFPTVNYTTMIHTPENPVIRYKRRSK
CYP51P1 human
no accession number
Rozman, D. Stromstedt, M. and Waterman, M.R. The three human
cytochrome P450 lanosterol 14a-demethylase (CYP51) genes reside on
chromosomes 3, 7, and 13: Structure of two retrotransposed
pseudogenes,
association with a line-1 element, and evolution of the human CYP51
family.
Archives of Biochemistry and Biophysics 333, 466-474 (1996).
processed pseudogene on chromosome 3
CYP51P1 human
processed pseudogene U36926 5 in frame stops
MAAAAGMMLLGLLQAGG*VLGQAMEEVAGGNLLSMLLIACAFTLSLVYLFRLAAGHLVQL
TAGAKSPPYIFSPVPFLGHAIAFGKSPTEFLENAYGNYGPVFSFIMVGKAFTYLLGSDAA
ALLFNSKNEVLNAEDVYSRLTTPVFG*GVAYDVPNPVFLEQKKTLKSGLNIAHFK*HVSI
IEKETKEYFESWGESGEKNVFAALSELIILTASHYLHGKEIRSQHNEKVAQLYADLDGGF
SHAAWLLPGWLPLPCFRRRDRAHQEIKDIFYKAIQKRRQSQEKIDDILQTLLDATYKDGR
PLTDDEVAGMLTGLLLAEQHTSSTSA*MGFFLARDKTLQEKCYLEQKTVCGENLPPLTY
DQLKDLNLLDRCIKETLRLRHPVMIMMRMARIPKTVAGYTIPPGHQVCVSPTVNQRLKDS
WVEHLDFNPDRYL*DNPASREKFAYVPFGAGHHGCTGENFAYVQIKTIWSTMLRLYEFDL
IDGYFPTVNYTTMIHTPENPVIHYK*RSK
CYP51P2 human
no accession number
Rozman, D. Stromstedt, M. and Waterman, M.R. The three human
cytochrome P450 lanosterol 14a-demethylase (CYP51) genes reside on
chromosomes 3, 7, and 13: Structure of two retrotransposed
pseudogenes,
association with a line-1 element, and evolution of the human CYP51
family.
Archives of Biochemistry and Biophysics, 333: 466-474 (1996)
processed pseudogene on chromosome 13
CYP51P2 human
processed pseudogene U40053
MAAAAGMMLLGLLQAG
GSVLGQAMEEVTGGNLLSMLLIACTFTLSLVYLFRLAAGHLVQLPAGAKSPPYVFSPVP
FPGHAIAFGKSPVEFLE
NAYEKYGPVFSFTMVGKTFTYL
LGSDAAALLFNSKNEDQNAEDVYSHLTTPVFGKGVAYDVPNPVFLEQKKMLKSGLNKAHF
KQHVSL
EKETKEYFQSWGESGEKNVFEALSELIILTASHCLHGKEIRSQLNEKVAQLYADLDGGFS
HAAWLLPGWLPLPSFRCRDRAHWEIKDIFYKAIQKRRQSQEKIDDILQTLLDATYKDGRP
LTDDEVAGMLIGLLLAGQHSSSTTSAWMDFFLARDKTLQEKCYLEQKTVCGENLPPLTYD
QLKGLNLLDRCIKETLRLRPPIMIMMRMARTPQTVVGYTIPPGHQVCVSPTVNQRPKDSW
VERLDFNPDCYLQDNPASGEKFAYVPFGAGCHR*IGENFAYVQIKTIWSTMLRLYEFDLI
DGYFPIVNYTTMIHTPENPLIHYKRRSK
CYP51P3 human
NT_025741.8|Hs6_25897 chromosome 6 CYP51P3
In an intron of the SASH1 gene
20447678 MLGLVQMSRSALGQLVEWVAGESDSLLSMLLISCAFILSLVCFATIVTTWPSCQLVQNAH 20447857
20447858 HMFSLPLHSLGMPYIWEKLN*ISRKCI*EVWAYEKYGPVCSFSVVSKTFT 20448007
20447859 ICFLSHYIPWACHTFGKS*IEFLESAYEKYGHMRSMDLYVVFLW*ARHLLLERDETAL 20448032
20448347 LFNSKTKDAEDVYSHLRTPAFGKGVECDMPNPAFLGQEKMLKSSLKVAHFRQQVSI 20448514
20448515 TEKERNTFKLGRKQRKKLCEALSELII 20448595
FDS*PWFTWKGNQKSTQ*EVAQLCADVSGGFKPLA 20448698
20448699 WLRPRWLPLRGCRSSYKAH*EHNYCL*GNPETQKPEEKIQGILQTLLDTTDKGEHLLAD 20448875
20448876 EEVTGLLIRLFSGQHTSSTTGA*MSCFVARDETLHEKCYLKQKTVRGEDLPSLTYDLLK 20449052
20449426 VIGRTIPAGHQMGLSLTVNQGFQDTWVELVDPDQ*LQDISTGEK 20449557
20449566 GGGRHHIGKNFAHVQIKTVWSTLFHLYEFDFIDGYFPTVNYKTVVHHTPKNPVITYK*R 20449742
CYP51P3 Cavia porcellus (Guinea pig)
cavPor3_dna scaffold_60:1743956-1873082 at UCSC browser
32% to 51 human in orthologous position to CYP51P3 pseudogene
inside SASH1 gene
60284 VVAEVGWLLLGFWQVSRLVVRWVVEQVTGASPCPY*PSPGPLSWAGLSASHL* 60126
60125 SPGTAAGGTESPSYLSLCSIPWAH*GCALAFW*CPAEFLENRV*EEWACLEFLWGEEEIY 59946
59945 FLVESDAAALLFKSKTEELNEEVHS 59871 CVPASGFQEG 59841
59545 VFIVETETKE*SNVGEEVEKIMYMSNFHSQS*F*KEIRSPSMWPWCSSYGA 59393
59392 IWSC*PFT
59368 WLLLGWQPWPRFSAATELTEASRTLSVRPSQSADTHEI*KKSSDSLHPLLKSTDKTGCPV 59189
59188 ACDEEAGMLT*LSFSR*LTSMTGF*MELIVARQNASRKRDL*QETVCRKALLPVTCDQL 59012
59011 RALNLLAHCIRQTSRPQ*DSSDCGKVSLFFQDSDYVFQPTGLES*WMH 58868
58867 ALKSLGDKFAHMPFRARHHCTGE 58799
58799 NFAYVQTRTV*FATLHWCGFDLINRFFPTINYTTMIRIPENLV 58671
CYP51A1 rat
GenEMBL D29962 (1924bp) PIR JC2334 (430 amino acids)
GenEMBL D55681 (2268bp)
Aoyama,Y., Funae,Y, Noshiro,M., Horiuchi,T. and Yoshida,Y.
Occurrence of a P450 showing high homology to yeast lanosterol 14-
demethylase (P450DM) in rat liver.
Biochem. Biophys. Res. Commun. 201, 1320-1326 (1994)
Note: This is the first time a single P450 family is represented in
plants and animals. The N-terminal is more conserved than the C-
terminal.
CYP51A1 rat
GenEMBL U17697 (2383bp)
Sloane,D.L., So,O., Leung,R., Scarafia,L.E., Saldou,N., Jarnagin,K.
and Swinney,D.C.
Molecular Cloning and Functional Expression of Rat Lanosterol
14 alpha-Demethylase
Gene (1995) In press
CYP51A1 rat
Aoyama, Y. et al . Sterol 14-demethylase P450 (P45014DM) is one of
the most ancient and conseved P450 species.
The Journal of Biochemistry 119: 926-933 (1996)
includes a report on a processed pseudogene from CYP51 in rats
Cyp51 mouse
GenEMBL NM_020010 AF166266 Mm.24155
MEQVTGGNLLSTLLIACAFTLSLVYLFRLAVGHMVQLPAGAKSP
PHIYSPIPFLGHAIAFGKSPIEFLENAYEKYGPVFSLTMVGKTFTYLLGSDAAALLFN
SKNEDLNAEEVYGRLTTPVFGKGVAYDVPNAIFLEQKKIIKSGLNIAHFKQYVPIIEK
EAKEYFQSWGESGERNVFEALSELIILTASHCLHGKEIRSQLNEKVAQLYADLDGGFT
HAAWLLPAWLPLPSFRRRDRAHREIKNIFYKAIQKRRLSKEPAEDILQTLLDSTYKDG
RPLTDEEISGMLIGLLLAGQHTSSTTSAWMGFFLAKDKPLQEKCYLEQKAVCGEDLPP
LTYDQLKDLNLLDRCIKETLRLRPPIMTMMRMAKTPQTVAGYTIPPGHQVCVSPTVNQ
RLKDSWAERLDFNPDRYLQDNPASGEKFAYVPFGAGRHRCVGENFAYVQIKTIWSTML
RLYEFDLINGYFPTVNYTTMIHTPENPVIRYKRRSK
CYP51P3 Cavia porcellus (Guinea pig)
cavPor3_dna scaffold_60:1743956-1873082 at UCSC browser
32% to 51 human in orthologous position to CYP51P3 pseudogene
inside SASH1 gene
60284 VVAEVGWLLLGFWQVSRLVVRWVVEQVTGASPCPY*PSPGPLSWAGLSASHL* 60126
60125 SPGTAAGGTESPSYLSLCSIPWAH*GCALAFW*CPAEFLENRV*EEWACLEFLWGEEEIY 59946
59945 FLVESDAAALLFKSKTEELNEEVHS 59871 CVPASGFQEG 59841
59545 VFIVETETKE*SNVGEEVEKIMYMSNFHSQS*F*KEIRSPSMWPWCSSYGA 59393
59392 IWSC*PFT
59368 WLLLGWQPWPRFSAATELTEASRTLSVRPSQSADTHEI*KKSSDSLHPLLKSTDKTGCPV 59189
59188 ACDEEAGMLT*LSFSR*LTSMTGF*MELIVARQNASRKRDL*QETVCRKALLPVTCDQL 59012
59011 RALNLLAHCIRQTSRPQ*DSSDCGKVSLFFQDSDYVFQPTGLES*WMH 58868
58867 ALKSLGDKFAHMPFRARHHCTGE 58799
58799 NFAYVQTRTV*FATLHWCGFDLINRFFPTINYTTMIRIPENLV 58671
CYP51A1 Gallus gallus (chicken)
DKFZ426_22O10R1 from http://www.ri.bbsrc.ac.uk/cgi-bin/est-blast/blast.pl
Roslin Institute chicken EST project
603372085F1>603372085F1 cloneID='ChEST280i22' Chondrocytes from http://www.chick.umist.ac.uk/
603582971F1>603582971F1 cloneID='ChEST534n18' stage_36_trunks from http://www.chick.umist.ac.uk/
data from the UMIST/Nottingham/Dundee universities chicken EST sequencing project.
82% to human CYP51 81% to Fugu CYP51
MLSLLEVGGSLLERAAVGNPLSLLLAASAFALSLGYLFQLGYRRHVGADRTNH
PPHIPSSIPFLGHAIAFGKSPIEFLENAYDKYGPVFSFTMVGK
TFTYLLGSDAAALLFNSKNEDLNAEDVYSRLTTPVFGKGVAYDVPNVVFLEQKKMLKTGL
NIAQFKQHVTLIEEETKEYFKAWGESGERNLFEAFSELIILTASHCLHGKEIRSLLNEKV
AQLYADLDGGFTHAAWLLPAWLPLPSFRRRDRAHRAIKNIFYKVIQKRRSSEEKEDDMLQ
TLLDASYKDGRPLTD
DEIAGMLIGLLLAGQHTSSTTSAWLGFFIARDKAIQEQCYAEQKAVCGDDLPPLTYDQLK
DLSLLDRCLKETLRLRPPIMTIMRLAKTPQTVAGYSIPPGHQVCVSPTVNQRLKDSWKDA
LDFKPDRYLRDNPAAGEKFAYIPFGAGRHRCIGENFAYVQIKTIWSTLLRLYEFDLVDGY
FPSINYTTMIHTPNNPVIRYKRRSL*
CYP51A1 Xenopus laevis (African clawed frog)
No accession number
Hiroaki Ohi, Yoshiaki Fujita
78-80% identical to mammalian CYP51s
submitted to nomenclature committee July 26, 2000
CYP51A1 Xenopus tropicalis
See Xenopus pages for seq
CYP51A1 Danio rerio (zebrafish)
Seq zebrafish pages for seq
CYP51A1 Fugu rubripes (pufferfish)
No accession number
Scaffold_437
78% to 51 mouse
67511 MSAHLYEMSSKLIGDTVGRVHD
67445 NLTTVVLAASFITLSLGYVSKLLLRQSFVTDA (0)
KHPPYI 67266
67265 PSCIPFLGHAISFGKSPIEFLENAYEK 67185
66989 YGPVFSFTMVGSTFTYLLGSDAAALLFNSKNEDLNAEDVYSRLTTPVFGKGVAYDVPNP 66813
IFLEQKKMLKTGLNIAHFKEHVKIIEAETRE 66633
66632 YFQRWGDSGER 66600
66525 DLFEALSELIILTASSCLHGKEIRSMLDERVAQLYADLDGGFTHAA 66388
WLLPGWLPLPSF
66277 RKRDRAHREIKKIFFKVIEKRRRSGENTDDILQTLVDATYK
DGRPLSDDEIGGMLIGLLLAGQHTSSTTSAWMGFFMARDRRLQ 65918
65917 ERCYAEQKAACGEDLPPLSFDQ 65852
LKDLSLLEGCLKETLRLRPPIMTMMRMARSPQ
TAAGYTIPVGHQVCVSPTVNHRLGDAWEQRLEFKPDRYLDDNPAAGEKFAYI 64964
64963 PFGAG
RHRCIGENFAYVQIKTIWSTLLRMYEF 64784
64783 DLVDGYFPTINYTTMIHTPHNPVIRYKRRHE* 64688
CYP51A1 Monosiga brevicola (choanoflagellate)
single celled sister group to all animals)
From JGI site estExt_fgenesh1_pg.C_250046 [Monbr1:38433]
51% to CYP51A1 of human
MDKLPAAVVPYAEAAQEALVSLHETLGRPSTTTYLATSAVALGIWKYIRGNYLRPAKAPPKVPSQVPWLG
CIFAFGQSPIEFMIDCYKKYGPVYSFVMFGTEVTYLLGSEASSRFWSTHNDVLNAEDLYANITVPVFGEG
VAYAVEHKIFSEQKQMAKEGLTIDRFKAYTSMIEKETNGFIERWGQTGTIDFFDNMARMIIYTATRCLHG
NETREDFDEDVAKLYHALDGGFTPQAWFFPPWLPLPSFRRRDRAHRELKERFYKIIDRRRQKAEEGTQTD
LMHTFMTTPYKNVEDGRHLTTDEVSGMMIALLMAGQHTSSTVSSWLTCFITTTPGLEEKLYQEQVELFKR
RPGPLSYEHINEMPLLWACIRETLRLRPPIMSIMRRAREDYKVTVNGVEYVIPKGSQVCVSPTVNGRLED
EWEDPNTFNPYRFLKEEDGKLVVTEGEQITKGGKFKWVPFGAGRHRCIGFGFAQVQIRCIMSTILRKYKL
EMVSGKLPPINYTTMIHTPTEPIVRYTRR*
CYP51A1 Caenorhabditis elegans (nematode worm/ mammalian contamination)
no accession number
F54E5 contig 00150 this fragment's amino acid translation is identical
to human sequences U51688, AC000120 and rat sequence AB004091.
DVFEALSELIILTASHCLHGKEIRSQLNEKVAQLYADLDGGFSHAA
WLLPGWLPLPSFR
The human sequences U23942, U40053 and D55653 and the rat sequences
U17697, D55681, D87997, D78370 all have N instead of D at the first
amino acid. This may be a polymorphism in this gene. It is very
unlikely that this sequence is from C. elegans and it most likely
represents a contamination by a mammalian sequence.
CYP51B1 Mycobacterium tuberculosis
GenEMBL Z80226
This is named in the CYP51 family even though it is in bacteria
It always clusters with the other CYP51 sequences.
The activity of this enzyme has been demonstrated to be a 14 alpha
demethylase with preference for obtusifoliol.
Mike Waterman presented the crystal structure of this
protein at a recent meeting in Moscow in July 2000.
See the bacterial nomenclature page for more CYP51B sequences.
CYP51C1 Phaeodactylum tricornutum (diatom)
GenEMBL BI307668.1
estExt_Genewise1.C_chr_310065|Phatr2 at JGI
note: one of the few P450s from diatoms (stramenopiles)
another is CYP97E1 from Skeletonema costatum
The CYP51C subfamily is for CYP51s in the Chromista
(Stramenopiles/heterokonts)
55% to Ectocarpus 51C1
MIVALVVVTGLTALWFFLLRPRTGGKHAPPVVTSSPLVPIPVIGHIVEFFRSPHFMMQRCLKDHGKVFTIPI
FHKRLTFL
IGPEAQEIFFKANDDVLSQSEVYDFTRPVFGNDVVYDASKKNRQVQFQTMANGLRTARLKAYIPKIEQETRAYIKNWGDA
GQIDLLKALSELTILTASRCLHGEDVRTHIFKEVQELYHDLDHGLTPLTVFWPTAPSQAHRKRDVARKEMVRLFTKVIEE
RQLHPERSDGTDILSLFMDIKYKDGSAVTMDQVTGLLIALLFAGQHTSCITSTWTSLFIANDPTLVSRILAEQKTVLGGD
LNKPIEYEDLQNMELLHNCMREALRMCPTFIMILRKAERDVKIQSEGKSYVIPQGDMVVVSPTVSMRMKETFADPDTYDP
DRFAAPREEHKQPYAYMGFGGGLHSCMGQNFAFLQVKTIISVLLREYELERVEPGMPDIGYDDMVVGPKGDCTVRYRKRV
QS*
CYP51C1 Thalassiosira pseudonana (diatom)
No accession
From JGI genome project
scaffold 68 48% to 51G1 Arabidopsis
66% to CYP51C Phaeodactylum tricornutum
55% to Ectocarpus 51C1
See stramenopile pages for a tree
This N-term is revised
1242369 MPFGFGSSTGPSDNLLIGLTCLAIFSLFYAFFVVRPRTKGGPHAPPVVTSSPVSSLPVVG
TIVEFGKSPVKMVQRCYEDYGPVFTVPV 1242106
FHKRLTFLIGPEAQEPFFKAPDEVLSQN
EVYGFMKPVFGPGIVYDASKKNRQVQFQSMANGLRTARLKGYTAKIERETRQYLESWGES
GELDLFHALSELTILTASRCLHGDDVRENLFKEVSELYHDLDQGLTPLTVFFPNAPTKSH
MKRNAARAKMVELFSKVIKNRRDNPDVQHSDGTDILSIFMDVKYKDGSNITDEQVTGLLI
ALLFAGQHTSCITSTWTSLFILNNPAILKRIIAEQNDVFGSQPDADVDYKMVNEDMPLLH
NSMKEALRLCPPLILLIRYALKDVKVKAAGKDYTIPKGDMVLISPSVGMRIPEVFKEPNT
FDPDRFGPDREEDKSSPFAYMGFGGGMHSCMGQNFAFVQVKTILSVLFREFELEMVSETM
PDIDYEAMVVGPKGDCRVRYKRRQ*
CYP51C1 Ectocarpus siliculosus (brown algae)
Genoscope Ectocarpus siliculosus brown algae project
ESTs LQ0AAA4YI04FM1.SCF, LQ0AAA7YJ01FM1.SCF
LQ0AAB54YL14FM1.SCF, LQ0AAB79YH22FM1.SCF
LQ0AAB69YF16FM1.SCF, LQ0AAB65YF19FM1.SCF
LQ0AAA9YO17FM1.SCF, LQ0AAB58YH11FM1.SCF
LQ0AAB55YN02FM1.SCF, LQ0AAB70YE14FM1.SCF
LQ0AAB83YM18FM1.SCF, LQ0AAB2YA05FM1.SCF
59% to Diatom CYP51C1 Thalassiosira pseudonana (ortholog)
about 46% to Chlamydomonas CYP51G1
Ectocarpus sctg_6 1176507-1185087
CYP51D1 Dictyostelium discoideum (cellular slime mold)
GenEMBL AU033519
47% identical to S. bicolor CYP51 all the best matches are to CYP51
note: CYP51D is a subfamily for CYP51s from Dictyostelium and
other Mycetozoa
MIGTIAVLVIAILVIFAFKKSPSNIPPIVETIPFIGCFYQFAKNPLQLVRNSYDRLGEIF
TLHLMGFKMTFVLGPEAQALFFRGTDEELSPKEAYRFVTPVFGKGVVYDSETEIMYEQLR
FVKNGLVLSQLKKAVGIIQEETEKYFETKWGDSGEIDLLYEMNKLTILTASRCLMGKSIN
KSLGQSGQLADLYPNWKKVSIQFHSFSQISHYHHSKRETLPVLKSRHFPSIIQERRRSTD
DSVDDVLYTLMNSKYKDGSVLEDEQIVGLMIGLLFAGQHTSSITLTYTIFYLLNNLEYFD
ETQKDINDIVQKENQGEINFDGLKRMNRLETVIREVLRLHPPLIFLMRKVMTPMEYKGKT
IPAGHILAVSPQVGMRLPTVYKNPDSFEPKRFDVEDKTPFSFIAFGGGKHGCPGENFGIL
QIKTIWTVLSTKYNLEVGPVPPTDFTSLVAGPKGPCMVKYSKKQK*
CYP51E1 Trypanosoma brucei (African sleeping sickness parasite)
GenEMBL AZ217433 AQ948529 AQ950690 AL492728 AL481001 AZ217732
AQ948526 AQ647531 AL480043 AQ660405 AL495885 AZ212935 AL481816
Assembled from overlapping GSS fragments
33% to mammalian CYP51s
Note: CYP51E is a subfamily for CYP51s of Euglenozoa
Also AAHB01000003.1 T. brucei chromosome 11, comp (1400971-1402470)
MLLEVAIFLLTALALYSFYFVKSFNVTRPTDPPVYPVTVPILGHIIQFGKSPLGFMQECKRQL
KSGIFTINIVGKRVTIVGDPHEHSRFFLPRNEVLSPREVYSFMVPVFGEGVAYAAPYPRMREQ
LNFLAEELTIAKFQNFVPAIQHEVRKFMAANWDKDEGEINLLEDCSTMIINTACQCLFGEDLRKRLDARRFAQ
LLAKMESSLIPAAVFLPILLKLPLPQSARCHEARTELQKILSEIIIARKEEEVNKDSSTSDLLSGLLSAVYRDGTPMSLHEV
CGMIVAAMFAGQHTSSITTTWSMLHLMHPANVKHLEALRKEIEEFPAQLNYNNVM
DEMPFAERCARESIRRDPPLLMLMRKVMADVKVGSYV
VPKGDIIACSPLLSHHDEEAFPEPRRWDPERDEKVEGAFIGFGAGVHKCIGQKFGLLQVK
TILATAFRSYDFQLLRDEVPDPDYHTMVVGPTASQCRVKYIRRKAAAA*
CYP51E1 Trypanosoma brucei (African sleeping sickness parasite)
GenEMBL AF363026
Joubert,B.M., Nguyen,L.N., Matsuda,S.P.T. and Buckner,F.S.
Cloning and functional characterization of a Trypanosoma brucei
lanosterol 14 alpha-demethylase gene
Mol. Biochem. Parasitol. 117 (1), 115-117 (2001)
CYP51E1v1 Trypanosoma cruzi (Chagas disease parasite)
GenEMBL AY283022.1
Buckner,F.S., Joubert,B.M., Boyle,S.M., Eastman,R.T., Verlinde,C.L.
and Matsuda,S.P.
Cloning and analysis of Trypanosoma cruzi lanosterol
14alpha-demethylase
Mol. Biochem. Parasitol. 132 (2), 75-81 (2003)
Submitted by Sean M. Boyle for Fred Buckner Oct 25, 2001
83% to CYP51 of T. brucei
clone name CL13
CYP51-1 allele (only 3aa diffs to allele 2)
MFIEAIVLALTALILYSVYSVKSFNTTRPTDPPVYPVTVPFLGH
IVQFGKNPLEFMQRCKRDLKSGVFTISIGGQRVTIVGDPHEHSRFFSPRNEILSPREV
YTIMTPVFGEGVAYAAPYPRMREQLNFLAEELTIAKFQNFVPAIQHEVRKFMAENWKE
DEGVINLLEDCGAMIINTACQCLFGEDLRKRLNARHFAQLLSKMESSLIPAAVFMPWL
LRLPLPQSARCREARAELQKILGEIIVAREKEEASKDNNTSDLLGGLLKAVYRDGTRM
SLHEVCGMIVAAMFAGQHTSTITTSWSMLHLMHPKNKKWLDKLHKEIDEFPAQLNYDN
VMDEMPFAERCVRESIRRDPPLLMVMRMVKAEVKVGSYVVPKGDIIACSPLLSHHDEE
AFPNPRLWDPERDEKVDGAFIGFGAGVHKCIGQKFALLQVKTILATAFREYDFQLLRD
EVPDPDYHTMVVGPTLNQCLVKYTRKKKLPS
CYP51E1v2 Trypanosoma cruzi (Chagas disease parasite)
GenEMBL AY283023.1
Buckner,F.S., Joubert,B.M., Boyle,S.M., Eastman,R.T., Verlinde,C.L.
and Matsuda,S.P.
Cloning and analysis of Trypanosoma cruzi lanosterol
14alpha-demethylase
Mol. Biochem. Parasitol. 132 (2), 75-81 (2003)
Submitted by Sean M. Boyle for Fred Buckner Oct 25, 2001
4 amino acid difference to CYP51E1v1
CYP51-2 allele, clone name WTB9
MFIEAIVLALTALILYSVYSVKSFNTTRPTDPPVYPVTVPFLGH
IVQFGKNPLEFMQRCKRELKSGVFTISIGGQRVTIVGDPHEHSRFFSPRNEILSPREV
YTIMTPVFGEGVAYSAPYPRMREQLNFLAEELTIAKFQNFVPAIQHEVRKFMAENWKK
DEGVINLLEDCGAMIINTACQCLFGEDLRKRLNARHFAQLLSKMESSLIPAAVFMPWL
LRLPLPQSARCREARAELQKILGEIIVAREKEEASKDNNTSDLLGGLLKAVYRDGTRM
SLHEVCGMIVAAMFAGQHTSTITTSWSMLHLMHPKNKKWLDKLHKEIDEFPAQLNYDN
VMDEMPFAERCVRESIRRDPPLLMVMRMVKAEVKVGSYVVPKGDIIACSPLLSHHDEE
AFPNPRLWDPERDEKVDGAFIGFGAGVHKCIGQKFALLQVKTILATAFREYDFQLLRD
EVPDPDYHTMVVGPTLNQCLVKYTRKKKLPS
CYP51E1 Trypanosoma congolense
Sanger Center congo469h11.p1k, congo931d01.p1k,
congo605h04.p1k
MLLEIVIFLLTAAALYAVYFVKSFNTTCVTDPPVYPVVLPFVGHILQFGRNPLEFMRKCK
RELGSGIFTINILGKRVTIVGDPHEHSRFFLPRNEVLSPREVYSFMVPVFGEGVAYAASY
PRMREQLNFLAEELTIAKFQNFVPAIQHEVRKFMVAHWDKEEGEINLLEDCSTMIINTAC
QCLFGEDLRRRLDARRFARLLAMMESSLIPAAVFAPFLLRLPLPQSARCHEA
RAELQQILSEIVISRKKEEVNRDSSTSDLLSGLLNAVY
RDGTPMSLHEVCGMIVAAMFAGQHTSSITT
TWSMLHLMHPSNKRHLETLRGEVEEFPAQLNYNNVMDEMPF
AERCARESIRRDPPLLMLMRKV
MSDVKVGPYVVPKGDIIACSPLLSHHDEEAFPDPRHWDPEREEKVEGAFIGFG
AGVHKCIGQKFGLLQVKTVLATVFRDYDFQLLRDQVPDPDYHTMVVGPTYSQCRVKYIRRKVVTA*
CYP51E1 Trypanosoma vivax
Contig7098.1
86% to 51E1 T. brucei
MLLEVITFLLTVLVLYSVYFVVSFNVTRPTDPPVRH
VALPFLGHIIPFGKDPLGFMLECKRKLRSGVFTINIMGKRVTVVGDPREHSRFFLPRNEV
LSPREVYAFMVPVFGEGVAYAAPYPRMREQLNFLAEELTIAKFQNFVPAIQHEARKFLSE
NWNKDEGQVNLLEDCSAMIINTACQCLFGEDLRKRLDARRFAQLLAKMESSLIPAAVFMP
SLLLLPLPQSACCRDAREELQLILSEIILARKREEVNKDSGTSDLLSGLLSAVYRDGTPM
SLHEVCGMIVAAMFAGQHTSTITTTWSLLHLMHPSNRKHLETLRKEIEEFPAQLNYSNVM
DEMPFAERCARESIRRDPPLIMLMRKVLADVKVGSYVVPKGDIIACSPLLSHHDEEAFPD
PRRWDPEREEVIEGAFIGFGAGVHKCIGQKFGLLQVKTILATVLRDYDFELPRDEVPEPD
YHTMVVGPTSSQCRVRYIRRKKPAS*
CYP51E1 Leishmania major (Leishmaniasis parasite)
LM7_11c.1.Contig2 L. major Friedlin chromosome 7_11c GenEMBL AZ048391
Also GenEMBL AZ048391 partial seq
76% to T. brucei CYP51E1
123423 MIGEFFLLLTAGLALYGWYFCKSFNTTRPTDPPVVHGAMPFVGHIIQFGKDPLDFMLNAK 123244
123243 KKYGGVFTMNICGNRVTVVGDVHQHNKFFTPRNEILSPREVYSFMVPVFGEGVAYAAPY 123067
123066 PRMREQLNFLAEELTVAKFQNFAPSIQHEVRKFMKANWNKDEGEINILDDCSAMIINTAC 122887
122886 QCLFGEDLRKRLDARQFAQLLAKMESCLIPAAVFLPWILKLPLPQSYRCRDARAELQDI 122710
122709 LSEIIIAREKEEAQKDSNTSDLLASLLGAVYRDGTRMSQHEVCGMIVAAMFAGQHTSTIT 122530
122529 TTWSLLHLMDPRNKRHLAKLHQEIDEFPAQLNYDNVMEEMPFAEQCARESIRRDPPLIML 122350
122349 MRKVLKPVQVGKCVVPEGDIIACSPLLSHQDEEAFPNPREWNPERNMKLVDGAFCGFGAG 122170
122169 VHKCIGEKFGLLQVKTVLATVLRDYDFELLGPLPEPNYHTMVVGPTASQCRVKYIRKKA 121993
121992 AA 121987
CYP51E1 Leishmania infantum
Sanger Center Contig3290
8620 MIGELLLLLAAGLALYGWYFCKSFNTTRPTDPPVVHGTTPFVGHIIQFGKDPLGFMLKAK 8799
8800 KKYGGIFTMNICGNRITVVGDVHQHSKFFTPRNEILSPREVYSFMVPVFGEGVAYAAPYP 8979
8980 RMREQLNFLAEELTVAKFQNFAPSIQHEVRKFMKANWNKDEGEINILDDCSAMIINTACQ 9159
9160 CLFGEDLRKRLDARQFAQLLAKMESCLIPAAVFLPWILKLPLPQSYRCRDARAELQDILS 9339
9340 EIIIAREKEEAQKDTNTSDLLAGLLGAVYRDGTRMSQHEVCGMIVAAMFAGQHTSTITTT 9519
9520 WSLLHLMDPRNKRHLAKLHQEIDEFPAQLNYDNVMEEMPFAEQCARESIRRDPPLVMLMR 9699
9700 KVLKPVQVGKYVVPEGDIIACSPLLSHQDEEAFPNPREWNPERNMKLVDGAFCGFGAGVH 9879
9880 KCIGEKFGLLQVKTVLATVLRDYDFELLGPLPEPNYHTMVVGPTASQCRVKYIKKKKAA 10056
CYP51E1 Euglena gracilis
GenEMBL EC678643.1 EC676958.1 EC676055.1 (N-term ESTs)
EC672115.1 (C-term EST)
51% to CYP51G1 Arabidopsis
MKLGVALAGAAALIAAPVVIVAFLRPKRGTAPLVPAPPVVGSLFDFMRSPLKFV
KAAHEKYGDCFTVNICHKRLTFLIGPQAHQKFFKPNDQQLDQAKVYQFNVPVFGPGVVFD
ADLDRRLQQFGFIQKALKPHLMKTYVRQMVEEAEDYFSSWDQEGIIDLREKLSELVIMTA
SRCLMGKEVREQLFAEVARLYHDLDEGMQPISVFFPYLPIEKHRRRDIARLEMVKLFSKV
LEQR
NPDVYDPDRFAEPRMEDKKNGPFAFISFGGGRHTCLGEHFGMLQVKVIVAVLLQHFDLEL
IEGLPEPDYAAMIVGPKPIHIKYRRVPKRF*
Note: CYP51F is a subfamily for fungal CYP51 sequences
CYP51F1 Saccharomyces cerevisiae (yeast)
GenEMBL U10555 (24,431bp)
Johnston,M., Andrews,S., Brinkman,R., Cooper,J., Ding,H., Dover,J.,
Du,Z., Favello,A., Fulton,L., Gattung,S., Geisel,C., Kirsten,J.,
Kucaba,T., Hillier,L., Jier,M., Johnston,L., Keppler,D.,
Langston,Y., Latreille,P., Louis,E., Macri,C., Mardis,E.,
Mouser,L., Nhan,M., Rifken,L., Riles,L., St.Peter,H., Thornton,L.,
Trevaskis,E., Vaudin,M., Vaughan,K., Vignati,D., Wilcox,L.,
Willis,A., Wilson,R., Wohldman,P. and Waterston,R.
The complete sequence of Saccharomyces cerevisiae chromosome VIII
Science 265, 2077-2082 (1994)
YHR007c reverse complement of 20,961-22,553, ERG11 gene
CYP51F1 Saccharomyces cerevisiae (yeast)
GenEMBL T17568 (45bp)
Burns,N., Grimwade,B., Ross-Macdonald,P.B., Choi,E.-Y., Finberg,K.,
Roeder,G.S. and Snyder,M.
Large-Scale Analysis of Gene Expression, Protein Localization and
Gene Disruption in Saccharomyces cerevisiae
Genes Dev. 8, 1087-1105 (1994)
CYP51F1 Saccharomyces paradoxus
Spar_10379
98% to 51F1 S. cerevisiae
see fungal pages for seq
CYP51F1 Saccharomyces mikatae
Smik_9639
96% to CYP51F1 S. cerevisiae
see fungal pages for seq
CYP51F1 Saccharomyces kudriavzevii
Skud_Contig2067.14
96% to CYP51F1 S. cerevisiae
see fungal pages for seq
CYP51F1 Saccharomyces bayanus
Sbay_23211
93% to CYP51F1 S. cerevisiae
see fungal pages for seq
CYP51F1 Saccharomyces castellii
Scas_Contig699.20
78% to CYP51F1 S. cerevisiae
see fungal pages for seq
CYP51F1 Saccharomyces kluyveri
Sklu_Contig2436.11
79% to CYP51F1 S. cerevisiae
see fungal pages for seq
CYP51F1 Kluyveromyces waltii
kwal_154-g13.1
77% to CYP51F1 S. cerevisiae
see fungal pages for seq
CYP51F1 Kluyveromyces polysporus
Kpol_1004p61
81% to CYP51F1 S. cerevisiae
see fungal pages for seq
CYP51F1 Kluyveromyces lactis
76% to CYP51F1 Saccharomyces kluyveri
see fungal pages for seq
CYP51F1 Ashbya gossypii ATCC 10895
NP_984259.1
71% to CYP51F1 S. cerevisiae,
see fungal pages for seq
CYP51F1 Yarrowia lipolytica
CAG82748.1
63% to CYP51F1 S. cerevisiae
see fungal pages for seq
CYP51F1 Penicillium italicum
GenEMBL Z49750 (2053bp)
Nistelrooy,H.G.M., Van den Brink,H.M., Kan,J.A.L., Gorcom,R.F.M.
and Waard,M.A.
Isolation and molecular characterisation of the gene encoding
eburicol 14-alpha-demethylase (CYP51) from Penicillium italicum
Unpublished (1995)
CYP51F1 Uncinula necator (grape powdery mildew fungus)
GenBank U72657 (1960bp) U83840 (1756bp) U72658 (1575bp)
Delye,C., Laigret,F. and Corio-Costet,M.F.
Cloning and sequence analysis of the eburicol 14alpha-demethylase
gene of the obligate biotrophic grape powdery mildew fungus
Gene 195 (1), 29-33 (1997)
note: a mutation causing resistance to an inhibitor triadimenol is
Y136F
Delye,C., Laigret,F. and Corio-Costet,M.F.
A mutation in the 14 alpha-demethylase gene of Uncinula necator that
correlates
with resistance to a sterol biosynthesis inhibitor.
Applied and Environ. Microbiol. 63, 2966-2970 (1997)
CYP51F1 Schizosaccharomyces pombe
GenEMBL Z54096 (12168bp)
Hunt,S. Devlin,K. and Churcher,C.M.
unpublished (1995)
CYP51F1 Schizosachharomyces japonicus
SJAG_03750
78% to Schizosachharomyces pombe CYP51A1
see fungal pages for seq
CYP51F1 Schizosachharomyces octosporus
SOCG_01956
see fungal pages for seq
CYP51F1 Pneumocystis carinii
AY228706
contig_349-snap.1 from Fungal Genome DB
contig_349-snap.2 from Fungal Genome DB
58% to CYP51F1 S. pombe
see fungal pages for seq
CYP51F1 Ustilago maydis
GenEMBL Z48164 (1874bp)
Hargreaves,J.A. and Keon,J.P.R.
The sterol 14 alpha demethylase (ERG11) gene from Ustilago maydis.
Unpublished (1994)
CYP51F1 Ustilago maydis
GenEMBL XM_401277
locus_tag="UM03662.1
CYP51F1 Puccinia graminis f. sp. tritici
PGTG_07202 revised
55% to CYP51F1 Malassezia globosa with a short seq gap.
see fungal pages for seq
CYP51F1 Malassezia globosa
MGL_2415
67% to CYP51F1 Ustilago maydis,
see fungal pages for seq
CYP51F1 Sporobolomyces roseus
JGI model e_gw1.1.480.1
55% to CYP51F1 Ustilago maydis,
see fungal pages for seq
CYP51F1 Issatchenkia orientalis
GenEMBL S75391 (1242bp)
Burgener-Kairuz,P. Zuber,J.P., Buchman,T.G., Bille,J. and Rossier,M.
Rapid detection and identification of Candida albicans and
Torulopsis (Candida) glabrata in clinical specimens by species-specific
nested PCR amplification of a
cytochrome P-450 lanosterol-alpha-demethylase (L1A1) gene fragment.
J. Clin. Microbiol. 32, 1902-1907 (1994)
CYP51F1 Kluyveromyces marxianus
GenEMBL X97682 (338bp)
Morace,G., Posteraro,B., Sanguinetti,M., Lo Cascio,G. and Fadda,G.
Identification of various medically important Candida species in
clinical specimens by using PCR-REA.
Unpublished
88% to CYP51F1 Kluyveromyces lactis
CYP51F1 Erysiphe graminis f. sp. hordei eburicol
GenEMBL AF052515 (2167bp)
Delye,C., Bousset,L. and Corio-Costet,M.F.
PCR cloning and detection of point mutations in the eburicol
14alpha-demethylase (CYP51) gene from Erysiphe graminis f. sp.
hordei, a 'recalcitrant' fungus.
Curr. Genet. 34, 399-403 (1998)
CYP51F1 Candida tropicalis
GenEMBL M23673
Chen,C., Kalb,V.F., Turi,T.G. and Loper,J.C.
Primary structure of the cytochrome P450 lanosterol 14
alpha-demethylase gene from Candida tropicalis
DNA 7 (9), 617-626 (1988)
CYP51F1 Candida glabrata
GenEMBL S75389 (1242bp)
Burgener-Kairuz,P. Zuber,J.P., Buchman,T.G., Bille,J. and Rossier,M.
Rapid detection and identification of Candida albicans and
Torulopsis (Candida)
glabrata in clinical specimens by species-specific nested PCR
amplification of a
cytochrome P-450 lanosterol-alpha-demethylase (L1A1) gene fragment.
J. Clin. Microbiol. 32, 1902-1907 (1994)
CYP51F1 Candida glabrata S75389
GenPept CAG58795.1
see fungal pages for seq
CYP51F1 Candida parapsilosis
GenEMBL AF019902 (677bp)
Okhravi,N., Adamson,P., Mant,R., Matheson,M.M., Midgley,G.,
Towler,H.M. and Lightman,S.
Polymerase chain reaction and restriction fragment length
polymorphism mediated detection and speciation of Candida spp
causing intraocular infection.
Invest. Ophthalmol. Vis. Sci. 39 (6), 859-866 (1998)
CYP51F1 Candida parapsilosis
CPAG_03310
75% to CYP51F1 Candida tropicalis
see fungal pages for seq
CYP51F1 Candida albicans
GenEMBL X13296, orf19_922
Lai,M.H. and Kirsch,D.R.
Nucleotide sequence of cytochrome P450 L1A1 (lanosterol 14
alpha-demethylase) from Candida albicans
Nucleic Acids Res. 17, 804 (1989)
CYP51F1 Candida guilliermondii
GenEMBL X97680 (336bp)
Morace,G., Sanguinetti,M., Posteraro,B., Cascio,G.L. and Fadda,G.
Identification of various medically important Candida species in
clinical specimens by PCR-restriction enzyme analysis
J. Clin. Microbiol. 35 (3), 667-672 (1997)
CYP51F1 Candida guilliermondii
PGUG_03415.1
71% to CYP51F1 Candida lusitaniae
see fungal pages for seq
CYP51F frag. Candida guilliermondii X97680
this seq is 94% to Candida albicans but only 66% to
full length CYP51A1 for C. guilliermondii
This is a different gene
MTDQEIANLLIVILMGGQHTSTSAWFLLHLGEKPHLQDVIYQEV
VELLKEKGGDLNDLTYEDLQKLPSVNNTIKETLRMHMPLHSTFRKVTNPLRSPNKYMS
SHTVYMFKFL
CYP51F1 Candida dubliniensis P450L1A1, partial.
GenEMBL AJ012573
Morace,G., Posteraro,B., Sanguinetti,M. and Fadda,G.
Identification of various medically important yeast by Reverse
Cross Blot Hybridization
Unpublished
MTDQEIANLLIGILMGGQHTSASTSAWFLLHLGEKPHLQDAIYQ
EVVELLKEKGGDLNDLTYEDLQKLPSVTNTIKETLRMHMPLHSIFRKVKNPLRIPETNYVVPRGHYVLVSPG"
CYP51F1 Candida dublinensis
93% to CYP51F1 Candida albicans, cdub_2-g61.1, GenEMBL AJ012573
see fungal pages for seq
CYP51F1 Candida lusitaniae
CLUG_04932.1
72% to CYP51F1 Candida tropicalis
see fungal pages for seq
CYP51F1 Lodderomyces elongisporus
LELG_03738
77% to CYP51F1 Candida parapsilosis
see fungal pages for seq
CYP51F1 Pichia anomala
GenEMBL AF019903
Okhravi,N., Adamson,P., Mant,R., Matheson,M.M., Midgley,G.,
Towler,H.M. and Lightman,S.
Polymerase chain reaction and restriction fragment length
polymorphism mediated detection and speciation of Candida spp
causing intraocular infection
Invest. Ophthalmol. Vis. Sci. 39 (6), 859-866 (1998)
CYP51F1 Pichia stipitis
JGI model e_gww1.7.1.351.1
71% TO CYP51F1 Debaryomyces hansenii
see fungal pages for seq
CYP51F1 Debaryomyces hansenii
GenPept CAG88416.1
75% to CYP51F1 Candida guilliermondii
see fungal pages for seq
CYP51F1 Neurospora crassa
No accession number
Neurospora crassa sequence contig 1.721 (supercontig 99)
Lower case = EST support
49163 MGILQVVAGPLSQQFSQLGTVSQIGVAIASFLFVAVVLNVLQQFLFKKPNEPPLVFHWFP 49342
49343 LIGSTITYGMDPPRFFKENREK (0)
YGDCFTFILLGKKT 49522
49523 TVYVGPKGNDFILNGKIRDVNAEEIYTVLTTPVFGKDVVYDCPNsklmeqkk 49678 (0)
49741 fmkialtteafrqyvpiisdevtsylkrtadfkgksgivdippkmaqitiftashalqg 49917
49918 keirdkfdetladlyhdldmgfspinfmlhwaplpwnnrrdyaqrtvakiymdtikerra 50097
50098 rgetgaqdimwhlmnstykgdvpvpdheiahmmiallmagqhsssstsswimlrlasr 50271
50272 pdimedlyneqvknlgadlppltyedlaklplhaaivkETLRLHAPIHSIMRAVKTPMPV 50451
50452 PGTKYVIPTDHVLLAAPGVSATDESYFPQPDLWEPHRWEKDSPLAPTIVRNVPSEEDDEK 50631
50632 IDYGYGLVSKGANSPYLPFGAGRHRCIGEQFANVQLQTILAIIVRNFKFRNVDGSDkvig 50811
50812 tdyaslfsrplepakiywerregcql* 50892
CYP51F1 Neurospora discreta
JGI gene model estExt_fgenesh3_kg.C_20410
98% to CYP51F1 N. crassa
see fungal pages for seq
CYP51F1 Aspergillus nidulans
AACD01000145 (WGS section)
AN8283.2
51 clan
MGLVSLVLDNVCERCSALSVWALSGLGLLSVIIIAVVLNVLRQILFKNPN
EPPVVFHWFPFIGSTISYGIDPYKFFFNCRAQYGDIFTFVLLGKKTTVYL
GTKGNDFILNGKLKDVCAEEVYSPLTTPVFGRHVVYDCPNAKLMEQKKFV
KYGLTSDALRSYVQLITAEVEDFAQKSSVFQNAKGVFDVSRTIAEITIYT
ASRSLQGKEVRDKFDSTFAELYHDLDMGFAPINFMLPYAPLPHNRKRDAA
QRKMAETYMEIIKERRKSGEKKDSEDMVWNLMSCVYKNGTPLSDEEIAHM
MIALLMAGQHSSSSTLSWILLHLARHPEIVEELYQEQLKVLGSDMHMTYD
DLQKLELHSKIIKETLRIHAPIHSIIRAVKSPMPVPGTSYVIPTSHNVLS
SPGVTARSDEFFPNPLKWDPHRWDSNPIANSTEDEEKIDYGYGLVSKGTN
SPYLPFGAGRHRCIGEQFAYVQLITVTAALVRLFKFDTVSESDKSSVPET
DYSSLFSRPAGKCFVQYEKRNVTTKA*
CYP51F1 Magnaporthe grisea
AACU01001110 cont2.837
MG04432.4 (version4) 75% to CYP51
MGLLQDTTGPLVDAFYQLGTGAQVGVAFVSFIFLSVFFHVAQQIFFKNPH
EPPVVFSWFPVVGSTVTYGKDPPQFFRDMAKKYGNIFTFILLGKKTTVYI
GTEGNEFILNGKLRDVNAEEIYGPMTTPVFGKDVVYDCPNAKLMEQKKFM
KIALTTEAFRSYVPIIADEVSSYLKRTPAFKGPSGVVNIPPKMAEITIFT
ASHALQGKEIRDQFDETLADLYHDLDMGFHPVNFKLHWLPLPRNIRRDKA
QKTIAKIYMDTIQRRRAKGKDSEAKDMMYHLMNSTYKNGTPVPDHEIAHM
MIALLMAGQHSSSSTSSWIMLRLASRPDIMEELYQEQVRALGADLPPLRY
EDLANLPLHLAVIKETLRLHAPINSILRAVKQDLPVPGTNYVIAKDTTVL
AAPGYSAGDPNHFPEPELWEPHRWEADSRLAPRISMSNDNDEEEKIDYGY
GLVSKGTTSPYLPFGAGRHRCIGEHFANVQLQTIVAMIVREFKFRNVDGS
GKVVGTNYASLFSRPEEPAKIYWERR*
CYP51F1 Fusarium graminearum
AACM01000048 (WGS section)
FG01000.1 FGcontig1.48_scaffold1
64% to Erysiphe graminis 72% to M. grisea 51F1
MGLLQELAGHPLAQQFQELPLGQQVGIGFAVFLVLSVVLNVLNQLLFRNP
NEPPMVFHWFPFVGSTITYGMDPPTFFRENRAKHGDVFTFILLGKKTTVA
VGPAGNDFILNGKLKDVCAEEIYTVLTTPVFGKDVVYDCPNAKLMEQKKF
MKIALTTEAFRSYVPIISSEVRDYFKRSPDFKGKSGIADIPKKMAEITIF
TASHALQGSAIRSKFDESLAALYHDLDMGFTPINFMLHWAPLPWNRKRDH
AQRTVAKIYMDTIKERRAKGNNESEHDMMKHLMNSTYKNGIRVPDHEVAH
MMIALLMAGQHSSSSTSSWIMLRLAQYPHIMEELYQEQVKNLGADLPPLT
YEDLAKLPLNQAIVKETLRLHAPIHSIMRAVKSPMPVPGTKYVIPTSHTL
LAAPGVSATDSAFFPNPDEWDPHRWEADSPNFPRMASKGEDEEKIDYGYG
LVSKGSASPYLPFGAGRHRCIGEHFANAQLQTIVAEVVREFKFRNVDGGH
TLIDTDYASLFSRPLEPANIHWERRQ*
CYP51F1 Fusarium oxysporum
FOXG_00394
92% to CYP51F1 Fusarium graminearum
see fungal pages for seq
CYP51F1 Fusarium verticillioides
98% to CYP51F1 Fusarium oxysporum
FVEG_01123
see fungal pages for seq
CYP51F1 Nectria haematococca (Fusarium solani group)
fgenesh1_pg.scaffold_2000426
Necha1/scaffold_2:1348428-1350174
genome annotation in progress
89% to Fusarium graminearum CYP51F1
FG01000.1 AACM01000048 FGcontig1.48_scaffold1
This gene model seems correct DRN 2/4/06
MGLLHEIAGHPLAQQFQELPLGQQVGIGFGAIVVLSVILNILSQILFVNPNEPPMVFHWFPFIGSTVTYG
MDPPRFFKENRAK (0)
FGDVFTFVLLGKKTTVAVGPSGNDFILNGKLKDVCAEEIYTVLTTPVFGKDVVYDCPNAKLMEQKK (0)
FMKIALTTEAFRSYVPIISGEVRDYFKKSADFKGKTGIVDIPKKMAEITIFTASHALQGSV
IRSKFDESLAALYHDLDMGFTPINFMLHWAPLPWNRKRDHAQRTVAKIYMDTIRERRIKDNDDSEHDMMK
HLMNSTYKNGTPVPDHEIAHMMIALLMAGQHSSSSTSSWIMLRLAQYPQIMEELYQEQVKALGADLPPLK
FEDLAKLPLNQAIIKETLRLHAPIHSIMRAVKSPMPVPGTKYVIPTSHTLLAAPGVSASDPAYFPNPDEW
DPHRWEAGSPNAPTIARNNAEEEEKIDYGYGLVSKGSASPYLPFGAGRHRCIGEHFANVQLQTIVAEVVR
EFKFSNVDGGNTLIGTDYASLFSRPLEPANIRWERRQ*
CYP51F1 Aspergillus fumigatus
GenEMBL AAK73660.1 also XP_749134.1, EAL87096.1
14-alpha sterol demethylase
79% to 51F1 A. nidulans
MGLIAFILDGICKHCSTQSTWVLVGIGLLSILAVSVIINVLQQLLFKNPHEPPVVFHWFPFIGSTISYGI
DPYKFFFDCRAKYGDIFTFILLGKKTTVYLGTKGNDFILNGKLRDVCAEEVYSPLTTPVFGRHVVYDCPN
AKLMEQKKFVKYGLTSDALRSYVPLITDEVESFVKNSPAFQGHKGVFDVCKTIAEITIYTASRSLQGKEV
RSKFDSTFAELYHNLDMGFAPINFMLPWAPLPHNRKRDAAQRKLTETYMEIIKARRQAGSKKDSEDMVWN
LMSCVYKNGTPVPDEEIAHMMIALLMAGQHSSSSTASWIVLRLATRPDIMEELYQEQIRVLGSDLPPLTY
DNLQKLDLHAKVIKETLRLHAPIHSIIRAVKNPMAVDGTSYVIPTSHNVLSSPGVTARSEEHFPNPLEWN
PHRWDENIAASAEDDEKVDYGYGLVSKGTNSPYLPFGAGRHRCIGEQFAYLQLGTITAVLVRLFRFRNLP
GVDGIPDTDYSSLFSKPLGRSFVEFEKRESATKA
CYP51F1 Neosartorya fischeri NRRL 181
XM_001261294.1, NFIA_024690
98% to CYP51F1 A. fumigatus = ortholog
63% to CYP51F2 A. fumigatus
MGLIAFILDGICKHCSTQSTWVLVGVGLLSILAVSVIANVLQQL
LFKNPHEPPVVFHWFPFIGSTISYGIDPYKFFFDCRAKYGDIFTFILLGKKTTVYLGT
KGNDFILNGKLRDVCAEEVYSPLTTPVFGRHVVYDCPNAKLMEQKKFVKYGLTSDALR
SYVPLITDEVESFVKNSPAFQGHKGVFDVCKTIAEITIYTASRSLQGKEVRSKFDSTF
AELYHNLDMGFAPINFMLPWAPLPHNRKRDAAQRKLTETYMEIIKARRQTGSKKDSED
MVWNLMSCVYKNGTPVPDEEIAHMMIALLMAGQHSSSSTASWIVLRLATRPDIMEELY
QEQIRVLGSDLPPLTFDNLQKLDLHAKVIKETLRLHAPIHSIIRAVKNPMAVDGTSYV
IPTSHNVLSSPGVTARSEEHFPNPLEWDPHRWDEDIAASAEDDEKVDYGYGLVNKGTN
SPYLPFGAGRHRCIGEQFAYLQLGTITAVLVRLFKFRNLPGVDGVPDTDYSSLFSKPL
GRSFVEFEKRESATKA
CYP51F1 Aspergillus oryzae
GenEMBL BAE60239.1
79% to CYP51F1 Aspergillus nidulans, 60% to CYP51F4
MGILAVILDSVCERCSGSSLWMLSTVALLSILVVSVVINVLRQLLFKNYKEPPLVFHWFPFIGSTISYGM
DPYRFFFNCREKYGDIFTFVLLGKKTTVYLGTKGNDFILNGKLRDVCAEEVYSPLTTPVFGRHVVYDCPN
AKLMEQKKFVKFGLTSDALRSYVRLITEEVEDFVQKSSALQGPNGVFDVCKTIAEITIYTASRSLQGKEV
RSRFDSTFAELYHDLDMGFAPINFMLPWAPLPHNRKRDAAQKRMTETYMEIIKERRKAGSKKDSEDMVWN
LMSCMYKDGTPVPDEEIAHMMIALLMAGQHSSSSTAAWIVLHLAASPEITEELYQEQLRILGHDMPPLTY
ENLQKLDLHAKVIKETLRIHAPIHSIIRAVKNPMPVEGTPYVIPTSHNVLSSPGVTARSEEHFPDPLEWK
PHRWDEAIAVSSEDEEKVDYGYGLVTKGTNSPYLPFGAGRHRCIGEQFAYVQLGAITAALVRLFKFSNLP
GVQTLPDTDYSSLFSKPLGNSKIQFEKREPVTKA
CYP51F1 Aspergillus flavus
AFL2G_06478
100% to CYP51F1 Aspergillus oryzae
CYP51F1 Aspergillus niger
estExt_fgenesh1_pm.C_150008|Aspni1
84% to CYP51F1
MGLLAVVLDSLCERCSNTSVLVVLGFGLLSLLAVSVIFNILRQLFFKNPNEPPLVFHWFPFIGSTISYGMDPYKFFFDCR
AKYGDIFTFILLGKKTTVYLGTRGNDFILNGKLRDVCAEEVYSPLTTPVFGRNVVYDCPNAKLMEQKKFVKFGLTSDALR
SYVRLITGEVENFVEHSAAFKGSSGVFDVCKTIAEITIYTASRSLQGKEVRSRFDSTFAELYHDLDMGFAPINFMLPWAP
LPHNRKRDAAQKKMTETYMEIIKERRNGGNKKDSEDMVWNLMSCIYKDGTPVPDEEIAHMMIALLMAGQHSSSSTASWIV
LHLARNPQIMEELYAEQIRVLGSDLPPLTYDNLQKLDLHAKVIKETLRIHAPIHSIIRAVKNPMPVDGTPYVIPTSHNVL
SSPGVTARSEEYFPNPLKWDPHRWDETIAASAEEEDQIDYGYGLVSKGTNSPYLPFGAGRHRCIGEQFAYVQLGAITAAL
VRLFKFRNLPNVKDIPETDYSSLFSKPAGKSIIQFEKRATTIKS*
CYP51F1 Aspergillus clavatus NRRL 1
XM_001273213.1, ACLA_005420
89% to 51F1 S. fumigatus
61% to 51F2 A. fumigatus
MGLITDILDGVCKYCSTQSIWMLGGVGLLSLLAVAVVVNVLRQL
LFKNPNEPPLVFHWFPFIGSTISYGIDPYKFFFDCRAKYGDIFTFILLGKKTTVYLGT
KGNDFILNGKLRDVCAEEVYSPLTTPVFGRHVVYDCPNAKLMEQKKFVKFGLTSDALR
SYVHLITDELESFVKSSSAFQGPKGVFDVCKTIAEITIYTASRSLQGKEVRNKFDSTF
AELYHDLDMGFAPINFMLPWAPLPHNRKRDAAQRKLTETYMEIIKARRQAGNEKDSED
MVWNLMSCVYKNGVPVPDEEIAHMMIALLMAGQHSSSSTAAWIVLRLATRPDIMEELY
QEQLRVLGSDLPPLTYDSLQKLDLHAKVIKETLRLHAPIHSIIRAVKNPMPVEGTPYV
IPTSHNVLSSPGVTARSEEHFADPLEWNPHRWDEHIVENDEDEEKIDYGYGLVNKGTN
SPYLPFGAGRHRCIGEQFAYVQLGTITAGLARLFKFRNLPDIEGIPDTDYSSLFSKPL
GKSVVQFEKREPALKA
CYP51F1 Aspergillus terreus NIH2624
XM_001212028.1, ATEG_02850.1
MGVFADIIGTLCEHCSTLSIWALGGAVLLAVFVLSVVINVLRQL
LFKNPHEPPVVFHWVPWIGSTIRYGIDPYKFFFECRAKYGDIFTFVLLGKKTTVYLGT
KGNDFILNGKLRDVCAEEVYSPLTTPVFGRHVVYDCPNAKLMEQKKFVKFGLTSDALR
SYARLITAEVDDFVKNSPTFQQPKGTFDVCKTIAEITIYTASRSLQGKEVRNRFDSTF
AEMYHDLDMGFAPINFVLPWAPLPHNRKRDAAQKKMAETYMEIIKERRQAGGKKDSED
MVWNLMSCVYKDGTPLPDEEIAHMMIALLMAGQHSSSSTASWIVLRLAENPEITEELY
QEQLRVLGSDLPPLTWDSLQKLDLHAKVIKETLRIHAPIHSIMRAVKNPMPVEGTPYV
IPTSHNVLSSPGVTARSEEYFSEPLKWDPHRWDETAAPSTEEEEETIDYGYGLVTKGT
NSPYLPFGAGRHRCIGEQFAYVQLGAITAALVRLFKFSNPPGANKLPDTDYSSLFSKP
LGKSYVQFEKRETNTKA
CYP51F1P Aspergillus terreus
ATEG_10302.1 revised
71% to CYP51F1 Aspergillus terreus
see fungal pages for seq
CYP51F1 Mycosphaerella graminicola
Fungal P450 Database Mgr032
63% to CYP51F1 Aspergillus fumigatus
see fungal pages for seq
CYP51F1 Mycosphaerella fijiensis
JGI model e_gw1.3.1317.1
81% to CYP51F1 Mycosphaerella graminicola
see fungal pages for seq
CYP51F1 Uncinocarpus reesii
UREG_07804.1
78% to CYP51F1 Ajellomyces capsulatus
see fungal pages for seq
CYP51F1 Coccidioides immitis
CIMG_07469.2
89% to CYP51F1 Uncinocarpus reesii
see fungal pages for seq
CYP51F1 Ajellomyces capsulatus NAm1 (Histoplasma capsulatum)
XM_001540158.1
63% to CYP51F2 A. fumigatus
74% to CYP51F1 A. fumigatus
MGLLADVVARVCAHCSTLSPWAFLLTGSATLIVLSVIINVLHQL
LWKNPNEPPVVFHWFPIIGSTISYGIDPYKFFLNCREKYGDIFTFVLLGKKTTVFLGT
KGNDFILNGKLKDVCAEEVYSPLTTPVFGSHVVYDCPNSKLMEQKKFVKYGLTSESLR
SYVTLITDEFNQYIKTSPAFQGDKGVLDVCKSIAEITIYTASRSLQGKEVRSKFDSSF
AQLYHDLDMGFTPINFMLPWAPLPHNRKRDAAQQKMTKIYTDIIRQRREAGGKKDSED
MVWNLMSCVYKDGTPLPDIEIAHMMIALLMAGQHSSSSTFSWIILRLASCPHIIEELY
EEQKQVLGEDLPPLTYETLQKLNLNSHVIRETLRIHAPIHSILRAVKSPMPVDGTRYT
IPTTHNLLAAPGVTSRLPEHFPNPMTWDPHRWENPAMAPEGDQSDEKLDYGYGLVSKG
ANSPYLPFGSGRHRCIGEQFAYVQLGTLLVAIVRQLKLKKLDGETGVPATDYSSLFSK
PLGKPLVAWERRNPTQK
CYP51F1 Histoplasma capsulatum G217B, teleomorph: Ajellomyces capsulatus
HCB02684.1 model is a fusion protein, top part deleted
96% to CYP51F1 Ajellomyces capsulatus NAm1
MGLLADVVARVCAHCSTLSLWALLLAGSASFIVLSVIINVLHQLLWKNPNEPPVVFHWFPIIGSTISYGIDPYKFFLNCR
EKYGDIFTFVLLGKKTTVFLGTKGNDFILNGKLKDVCAEEVYSPLTTPVFGRHVVYDCPNSKLMEQKKFVKYGLTSESLR
SYVTLITDEFNQYIKTSPAFQGDKGVLDVCKSISEITIYTASRSLQGKEVRSKFDSSFAQLYHDLDMGFSPINFMLPWAP
LPHNRKRDAAQQKMTKIYTDIIRQRREAGGKKDSEDMVWNLMSCVYKDGTPLPDVEIAHMMIALLMAGQHSSSSTLSWII
LRLASCPHIIEELYEEQKQVLGEDLPPLTYETLQKLNLNSHVIRETLRIHAPIHSILRAVKSPMPVDGTRYTIPTTHNLL
AAPGVTSRLPEHFPNPMTWDPHRWENPAMAQEEDQSGEKLDYGYGLVSKGANSPYLPFGSGRHRCIGEQFAYVQLGTLLA
AIVRQLKLKKLDGETGVPATDYSPLGKPLVAWERRNPTQK
CYP51F1 Venturia inaequalis strain Ent27
GenEMBL AF227920
14 alpha-demethylase
MGLLSPLLAXLPGSDRSWLFYTLASFGFTVAIVAANLVKQLLFS
NPNEPPVVFHWFPFFGNTVVYGIDPIKFFAECKEKHGDIFTFILLGRKTTVYIGTKGN
EFILNGKQSHVNAEEIYSPLTTPVFGSDVVYDCPNSKLMEQKKFVKYGLTTE ALKSYV
TLIQQEVEDYTKRYPQFKGEKGSFDVCASMAEITIFTASRSLQGKEVRDKFDASFADL
FHDLDMGFSPINFMLPWAPLPHNRRRDAANKKMTETYLEIIQSRKAEGVKKDSEDMIW
NLMQCVYKNGTPIPDKEIAHMMIALLMAGQHSSSSTSSWILLRLATRPDIQEELYQEQ
IRVCGADLPPLQYEDLARMPLHNQIIKETLRMHSPIHSILRAVKQPMPVEGTPYTIPT
SHVLLAAPIASGGSPMYFPAPEKWEPHRWDEGSGGTNISGGENGGEEKEDYGYGLITK
GASSPYLPFGAGRHRCIGEQFAYMQLNTVLATQVREFKFSLREGESFPKTDFSSLFSG
PLRPAWLNWERREKSS
CYP51F1 Phanerochaete chrysosporium (white rot fungus)
Scaffold_44, JGI gene model ug.2.6.1
54% to Ustilago maydis CYP51F1
MSLSQYGPIAGLVGQAYDALASMSTSRLVLFLLINIPILSV
LPKDKSLPPVVWHWFPWFGSAAAYGEDPIKFFFDCKEK
YGNVFTFILMGRKVTVALTPAGNNFIMGGKHTTFSAEEVYG
GLTTPVFGKDVVYDCPNELLMEQKKFVKFGLSTENFRQYVGMIEEEVLQFMRNDASFK
IYQMNDINEWGAFDVLKVMSEITILTASRTLQGKEVRANITKDYAQVYNDLDGGFTPLHF
MFPNLPLESYRKRDAAHKKISDFYISIIRKRRENPGQ
EEHDMIAALMNQKYRVGRPLKDHEIAHIMIALLMAGQHTSSATGSWALLHIADRPDVA
EALYEEQVKHFRQSDGSWRTPEYEELKELPVLDSVIRETLRIHPPIH
SIMRAVREDVVVPPTLAAPSEDGRYVIPKGHVVLSSAAISQVDPMLWKNANDWDPSRWSD
PEGVAAQAYKQYDDAEGAKVDFGFGLVSKGTDSPYQPFGAGRHRCIGEQFAYLQLGTIISTFVR
HVEMRLPETGVPPPNYHVRSHTRILRLVCADVASADDDHTPEGASQHSLQAA*
CYP51F1 Cryptococcus neoformans var. neoformans B-3501A chromosome 1
56% to CYP51F1 Phanerochaete chrysosporium
AAEY01000001
CNBA0300
ESTs gb|CF192489.1
gb|CF192488.1, gb|CF191439.1
EAL23379.1
see fungal pages for seq
CYP51F1 Rhizopus oryzae (Zygomycete fungus, Mucoromycotina)
RO3G_11790.1
46% to CYP51F1 Aspergillus fumigatus = CYP51F1
see fungal pages for seq
CYP51F1P Rhizopus oryzae (Zygomycete fungus, Mucoromycotina)
RO3G_08504.1 revised
60% TO CYP51F1 Rhizopus oryzae
see fungal pages for seq
CYP51F1 Phycomyces blakesleeanus (Zygomycete fungus, Mucoromycotina)
JGI model estExt_fgeneshPB_pg.C_10567
73% to CYP51F1 Rhizopus oryzae
see fungal pages for seq
CYP51F1 Batrachochytrium dendrobatidis JEL423 (chytrid)
BDEG_01891
43% to CYP51F1 Rhizopus oryzae
note this species has no obvious CYP61 sequence
see fungal pages for seq
CYP51F2 Aspergillus nidulans
AACD01000029 (WGS section)
AN1901.2
64% to 51F2, 60% to 51F1
MLSPTLFSAYVLVGAIVAVLVNVIRQIFFRNKNEPPMVFHWVPFVGSTIS
YGMNPYKFFFSCREKYGDIYTFVMLGKKMTVYMGVKGNDFILNGKLKDLN
AEEVYSPLTTPVFGSDVVYDCPNSKLMEQKKFIKFGLSQSALESHVPLIE
KEVLDYIKTSPRFKGDSGVLDAPAAMAELTIYTAGSALQGKEVRKKLTAE
FADLFHDLEMGFTPINFILPWAPLPQNRKRDIAHARMRETYMEIINQRRK
NPDAQDHDMIWNLMHSTYKNGNPVPDKEIAHIMITLLMAGQHSSSSISAW
ILLRLASEPQILEELYQEQLANLKRDPRTGAFEPLQYKDLDLLPLHQNVI
KETLRVHLSIHSILRKVKNPIPVPDTPYIIPTSHTLLASPGATALSDEYF
PNANMWDPHRWENQRPDKEDEEGELIDYGYGAVSKRMSSPYLPFGGGRHR
CIGEKFAYVNLGVIVATIVRNLKLYNVDGKTGVPATDYSSMFMGPMKPAV
VGWERRFPARS*
CYP51F2 Magnaporthe grisea
AACU01001139 cont2.870
MG04628.4 (version4) 62% to CYP51 Penicillium
MAFFFPSAPVWVYSAGAALLFIIGSIILNFIWQQLPRPKSEPPLVFHWLP
FIGNAVSYGMDPYRFYSQCREKHGDVFTFVLFGRRMTVFLGVQGNDFILN
GKLQDLNAEEIYSPLTTPVFGSDIIYDCPNSKLMEQKKFVKFGLTQKALD
SYVPLIEREVLDYIESSPVFQAGNHGIVDIPSMMAEITIFTASRTLQGPE
VRKKLTGEFARLYHDLDLGFRPINFLAPWAPLPQNRRRDVAHARMRDVYM
DLINKRRRQKDDQEEEEEAEPDMIRHLMGSCVYKNGQALPDKEIAHMMIT
LLMAGQHSSSSSSAWIMLRLASRPDIAEEVYQEVQRLGHASLQHSDLDKL
PLLANVVKETLRVHSSIHSIMRKVKRPMRIPGSDYVVTPGKVLVSAPIMT
HLDEEHFRDARAWEPHRWDDAVDAQDDEIVDYGYGATSKGTKSPYLPFGA
GRHRCIGEKFAYLNLAAIVSTLVRNFKFSTLDGKATVPPTDYTSMFSRPM
QPATVRWERRSPKTA*
CYP51F2 Fusarium graminearum
AACM01000179 (WGS section)
FG04092.1 FGcontig1.179_scaffold2
60% to CYP51F1 Penicillium 68% to Mg CYP51F2 54% to Mg 51F1
MFHLLIYPLWVLVALFAVIIANLLYQQLPRRPDEPPLVFHWFPFFGNAVA
YGLDPCGFFEKCREKHGDVFTFILFGRKIVACLGVDGNDFVLNSRLQDAN
AEEVYGPLTIPVFGSDVVYDCPNSKLMEQKKFVKFGLTQKALESHVQLIE
REVLDYVETDPSFSGRTSTIDVPKAMAEITIFTASRSLQGEEVRRKLTAE
FAALYHDLDLGFRPVNFLFPWLPLPHNRKRDAAHIKMREVYMDIINDRRK
GGIRTEDGTDMIANLMGCTYKNGQPVPDKEIAHMMITLLMAGQHSSSSAS
SWIVLHLASSPDITEELYQEQLVNLSVNGALPPLQYSDLDKLPLLQNVVK
ETLRVHSSIHSILRKVKRPMQVPNSPYTITTDKVIMASPTVTAMSEEYFE
NAKTWNPHRWDNRAKEEVDTEDVIDYGYGAVSKGTKSPYLPFGAGRHRCI
GEKFAYVNLGVIVATLVRNFRLSTIDGRPGVPETDYTSLFSRPAQPAFIR
WERRKKI*
CYP51F2 Fusarium oxysporum
FOXG_11545
83% to CYP51F2 Fusarium graminearum
see fungal pages for seq
CYP51F2 Fusarium verticillioides
FVEG_10277
94% to CYP51F2 Fusarium oxysporum
see fungal pages for seq
CYP51F2 Nectria haematococca (Fusarium solani group)
fgenesh1_pg.scaffold_7000156
Necha1/scaffold_7:419968-421550
80% to Fusarium graminearum CYP51F2
57% to CYP51F1 like seq fgenesh1_pg.scaffold_2000426,
genome annotation in progress
Note only one intron, not two as seen in CYP51F1
MLLLVWYPVLAIGAFLGIILIHALRQKLFRKAGEPPLIFHWLPFIGNAVSYGLDPCNFFMKCREK (0)
HGDVFTFVLFGRKIVCCLGVEGNDFVLNSRLQDANAEEIYGPLTIPVFGSDVVYDCPNSKFMEQKKFVKFGLTQK
ALESHVQLIEREVLDYIHAVPAFSGSEGTVDISNAMAEITIFTAARSLQGEEVRRKLTAEFAALYHDLDL
GFKPVNFLFPWAPLPHNRKRDAAHAKMRDIYMDIINKRRAEGGDLGEYSDMIANLMGCTYKNGQPVPDKE
IAHMMITLLMAGQHSSSSASSWIMFRLASRPDIAEELYQEQLTHLSVDGYLPPLQHSDLDKLTLLQNVVK
ETLRVHSSIHSILRKVKTPMQAPGTQYTITTDKVILASPTVTALSEEYFSNARTWDPHRWDNKEKEEVAG
DDVVDYGYGKVSKGTKSPYLPFGAGRHRCIGEKFAYVNLGVIVATMVRNFKLSTMDGKSGVPATDYNSLF
SRPVQPAYIRWERRKA*
CYP51F2v1 Aspergillus fumigatus strain = ATCC 36607
GenEMBL AAF32372.1 also AAK73659.1
cytochrome P450 sterol 14 alpha-demethylase variant
MVPMLWLTAYMAVAVLTAILLNVVYQLFFRLWNRTEPPMVFHWVPFLGSTISYGIDPYKFFFACREKYGD
IFTFILLGQKTTVYLGVQGNEFILNGKLKDVNAEEVYSPLTTPVFGSDVVYDCPNSKLMEQKKFIKYGLT
QSALESHVPLIEKEVLDYLRDSPNFQGSSGRMDISAAMAEITIFTAARALQGQEVRSKLTAEFADLYHDL
DKGFTPINFMLPWAPLPHNKKRDAAHARMRSIYVDIINQRRLDGDKDSQKSDMIWNLMNCTYKNGQQVPD
KEIAHMMITLLMAGQHSSSSISAWIMLRLASQPKVLEELYQEQLANLGPAGPDGSLPPLQYKDLDKLPFH
QHVIRETLRIHSSIHSIMRKVKSPLPVPGTPYMIPPGRVLLASPGVTALSDEHFPNAGCWDPHRWENQAT
KEQENDEVVDYGYGAVSKGTSSPYLPFGAGRHRCIGEKFAYVNLGVILATIVRHLRLFNVDGKKGVPETD
YSSLFSGPMKPSIIGWEKRSKNTSK
CYP51F2v2 Aspergillus fumigatus Af293
GenEMBL XP_752137.1 also EAL90099.1
14-alpha sterol demethylase variant
74% to 51F2 A. nidulans 62% to 51F1 A. nidulans
only 5 aa diffs to CYP51F2v1, may be a stran variant
MVPMLWLTAYMAVAVLTAILLNVVYQLFFRLWNRTEPPMVFHWVPYLGSTISYGIDPYKFFFACREKYGD
IFTFILLGQKTTVYLGVQGNEFILNGKLKDVNAEEVYSPLTTPVFGSDVVYDCPNSKLMEQKKFIKYGLT
QSALESHVPLIEKEVLDYLRDSPNFQGSSGRVDISAAMAEITIFTAARALQGQEVRSKLTAEFADLYHDL
DKGFTPINFMLPWAPLPHNKKRDAAHARMRSIYVDIITQRRLDGEKDSQKSDMIWNLMNCTYKNGQQVPD
KEIAHMMITLLMAGQHSSSSISAWIMLRLASQPKVLEELYQEQLANLGPAGPDGSLPPLQYKDLDKLPFH
QHVIRETLRIHSSIHSIMRKVKSPLPVPGTPYMIPPGRVLLASPGVTALSDEHFPNAGCWDPHRWENQAT
KEQENDKVVDYGYGAVSKGTSSPYLPFGAGRHRCIGEKFAYVNLGVILATIVRHLRLFNVDGKKGVPETD
YSSLFSGPMKPSIIGWEKRSKNTSK
CYP51F2 Neosartorya fischeri NRRL 181
XM_001267337.1, NFIA_109350
97% to CYP51F2 Aspergillus fumigatus
MVPMLLLTAYMAVAMLTAILLNVVYQLFFRLWNRTEPPMVFHWV
PFLGSTISYGIDPYKFFFACREKYGDIFTFILLGQKTTVYLGVQGNEFILNGKLKDVN
AEEVYSPLTTPVFGSDVVYDCPNSKLMEQKKFIKYGLTQSALESHVPLIEKEVLDYLR
NSPNFQGSSGQVDISAAMAEITIFTAARALQGQEVRSKLTAEFADLYHDLDKGFTPIN
FMLPWAPLPHNKKRDAAHARMRSIYIDIINQRRLDGEKDSQKSDMIWNLMNSTYKNGQ
QVPDKEIAHMMITLLMAGQHSSSSISAWIMLRLASQPKVLEELYQEQLANLGPVGPDG
SLPPLQYKDLDKLPFHQHVVRETLRLHSSIHSLMRKVKSPLPVPGTPYMIPPGRVLLA
SPGVTALSDEHFPNAGCWDPHRWENQAAKEQENDEVVDYGYGAVSKGTSSPYLPFGAG
RHRCIGEKFAYVNIGVILATIVRHLRLFNVDGKKGVPETDYSSLFSGPMKPSIIGWEK
RSKDTSK
CYP51F2 Aspergillus oryzae
GenEMBL BAE57417.1
77% to CYP51F4, 75% to CYP51F2 Aspergillus nidulans
MASFTLVSAYAAAGLLAIIVLNLLRQLLFRNKTDPPLVFHWIPFLGSTVTYGMDPYAFFFSCRQKYGDIF
TFILLGRKITVYLGIQGNEFILNGKLKDVNAEEIYSPLTTPVFGSDIVYDCPNSKLMEQKKFIKFGLTQA
ALESHVPLIEKEVLDYLKTSPNFKGTSGRVEITDAMAEITIFTAGRALQGEEVRKKLTTEFADLYHDLDR
GFTPINFMLPWAPLPRNRKRDAAHARMREIYMDIINERRKNPDRETSDMIWNLMHCTYKNGQPLPDKEIA
HMMITLLMAGQHSSSSISSWIMLRLASEPAVMEELYQEQITKLSPDGRTLPPLQYRDLDLLPLHQNLIKE
TLRLHLSIHSLMRKVKNPMPVPGTPYVVPADHVLLASPGVTALSDEYFPNASRWDPHRWENRVEKEDEED
IVDYGYGTVSKGTSSPYLPFGAGRHRCIGEKFAYVNLGVIVATMARHMKLFNVDGKKGVPATDYSSMFSG
PSKPAIIGWERRFPEKS
CYP51F2 Aspergillus flavus
AFL2G_02771
99% to CYP51F2 Aspergillus oryzae, 1 aa diff
see fungal pages for seq
CYP51F2 Aspergillus clavatus NRRL 1
XM_001271578.1, ACLA_046180
81% to CYP51F2 Aspergillus fumigatus
MLSLTLFGLYLVSATAVVILVNVVYQHLFRLRNRTEPPMVFHWI
PFIGSTITYGIDPCKFFFACREKYGNIFTFILLGQKVTVYLGVEGNEFILNGKLKDVN
AEEVYSPLTTPVFGSDVVYDCPNSKFMEQKKFIKFGLTQSALEAHVPLIEKEVLDYLE
TSPRFQGTSGLVDIAAAMAEITIFTAARALQGEEVRSKLTAEFADLYHDLDRCFTPVN
FMFPWAPLPRNKKRDAAHVRMRAIYVDIINQRRRDGGEDTQKSDMIWNLMNCSYKNGQ
QVPDKEIAHMMITLLMAGQHSSSSIGSWIMLRLASQPEVLEKLYQEQLDKLAQGGPGS
NLRPLQYKDLELLPYHQHVIRETLRLHSSIHSILRKVKNTLSVPGTSYVIPPGRVLLA
SPGVTALSDEHFPNASRWDPQRWENQTTKEDSGEMVDYGYGAVSKGTASPYLPFGAGR
HRCIGEKFAYVNIGVILATLVRHLRLSNMDGKEGVPATDYSSLFSGPMKPSIIQWGKR
SNDLSK
CYP51F2 Aspergillus terreus NIH2624
XM_001215095.1, ATEG_05917.1
78% to CYP51F2 Aspergillus fumigatus
MSLLTITSYAAVGILALIAWNVIRQLLFQNKSEPPVVFHWIPFL
GSTISYGIDPYAFFASCRQKYGDIFTFILLGQKTTVYLGVQGNEFILNGKLKDVNAEE
VYSPLTTPVFGSDVVYDCPNAKLMEQKKFIKYGLTQAALESHVQLIEREVLDYLRTSP
NFHGASGVVDISAAMAELTIFTAGRALQGEEVRRKLTAEFADLYHDLDKGFTPINFML
PWAPLPHNRKRDAAHARMRAIYMDIIRERRAHKNPDQVEAGSDSDMIWNLMRCTYKDG
QPVPDKEIAHMMITLLMAGQHSSSSISAWIFLRLASEPRVLEELYQEQVASLGKPDAD
GVFPPLQFRDLDRLPLHQNVVKETLRLHSSIHSIMRKVKNPLPVPGTPYVVPTSHVLL
ASPGVTATSDEYFPNASRWDPHRWENQAEPDDDGEMVDYGYGRVSKGTASPYLPFGGG
RHRCIGEKFAYINLGVIVATVVRHLKLANVDGRKGVPQTDYSSLFSGPVKPAIIGWER
RFPTA
CYP51F2 Aspergillus niger CBS 513.88
XM_001394187.1, CAK40582.1
80% to CYP51F2 Aspergillus fumigatus
MALLAVAGVYAFAALLVAIVLNVTRQLLFRNEKEPPVVFHWIPF
LGSTISYGMDPYTFFFSCRKKYGDIFTFVLLGQKTTVYLGVQGNDFILNGKLKDVSAE
EVYSPLTTPVFGSDVVYDCPNSKLMEQKKFIKFGLTQAALESHVQLIEKETLDYLRDS
PRFNGASGVIDIPAAMAEITIYTAARALQGEEVRKKLTAEFAELYHDLDKGFSPINFM
LPWAPLPHNQKRDAAHARMREIYTDIINERRKNPDEEKSDMIWNLMHCTYKSGQPVPD
KEIAHMMITLLMAGQHSSSSISSWIMLRLASEPQVLEELYQEQLASLSNRNGVFEPLQ
YQDLDKLPFLQSVIKETLRIHSSIHSIMRKVKNPLPVPGTSYIIPEDHVLLASPGVTA
LSDEYFPNATRWDPHRWENQPDKEEDGEMVDYGYGSVSKGTASPYLPFGAGRHRCIGE
KFAYVNLGVIIATIVRHLKLFNVDGRKGVPGTDYSTLFSGPMKPAIVGWERRFPDHSK
GSLN
CYP51F2 Aspergillus niger (from JGI)
estExt_fgenesh1_pm.C_40090|Aspni1
80% to CYP51F2 Aspergillus oryzae,
66% to 51F1 Penicillium italicum
4 aa diffs to Aspergillus niger CBS 513.88
MALLAVAGVYAFAALLVAIVLNVTRQLLFRNEKEPPVVFHWIPFLGSTISYGMDPYTFFFSCRKKYGDIFTFVLLGQKTT
VYLGVQGNDFILNGKLKDVSAEEVYSPLTTPVFGSDVVYDCPNSKLMEQKKFIKFGLTQAALESHVQLIEKETLDYLRDS
PRFNGASGVIDIPAAMAEITIYTAARALQGEEVRKKLTAEFAELYHDLDKGFSPINFMLPWAPLPHNRKRDAAHARMREI
YTDIINERRKNPDEEKSDMIWNLMHCTYKSGQPVPDKEIAHMMITLLMAGQHSSSSISSWIMLRLASEPQVLEELYQEQL
ASLSNRNGVFEPLQYQDLDKLPFLQSVIKETLRIHSSIHSIMRKVKNPLPVPGTSYIIPEDHVLLASPGVTALSDEYFPN
ATRWDPHRWENQPDKEEDGEMVDYGYGSVSKGTASPYLPFGAGRHRCIGEKFAYVNLGVIIATIVRHLKLFNVDGRKGVP
GTDYSTLFSGPMKPAIVGWERRFPDNIKGSMN*
CYP51F2 Ajellomyces capsulatus NAm1
XM_001540591.1
70% to CYP51F2 Aspergillus fumigatus
MSWFTIGTFGVLAFVAAVTLNLVYQLLFRFLNKTRPPLVFHWIP
FLGSTIRYRTDPYKFFFSCRQKEASRLFQHGDIFTFVLLGRPTTVYLGIKGNEFILNG
KLKDVNAEEVYGPLTTPVFGPDVVYDCPNSKLIEQKKFIKYGLTQAALESHVPLIEKE
VMDYLDSSPNFLGASGEVDISAVMAEITIFTAGSALQGEEVRSKLTTEFAVLYHDLDK
GFSPINFMLPWAPLPHNKKRDAAHTLMHAIYLDIIEKRRRAARNANGSQTQDMIENLM
QCTYKNGQKLPDKEIANIMISLLMAGQHSSSTTSSWIMLHLASEPAVVEQLYQEQLDN
LPRTGPNGSLGPLQYGDLNRLPLHRNVIRETLRLHTSIHSLLRKVMNPMPVAGTPYVI
PPSHVVLSAPGVTALSDEYFPNPTMWDPNRWETQEPKVDEKEDMVDYGYGTISKGTSS
PYLPFGAGRHRCIGEKFAYVNLTVIVAIMVRHLQFSNIGGKTGVPRTDYSSMFSAPMK
PARINWKRRTAKSG
CYP51F2 Histoplasma capsulatum G217B
94% to CYP51F2 Ajellomyces capsulatus NAm1
two identical sequences
ABBT01000205.1 Ajellomyces capsulatus G217B 481620-479996 (-)
ABBT01000207.1 Ajellomyces capsulatus G217B 78633-77009 (-)
HCB05685.1 identical to HCB06140.1
see fungal pages for seq
CYP51F2 Penicillium digitatum strain GL-01
DQ355161.1, also AJ439081 (5 aa diffs)
67% to CYP51F2 Aspergillus fumigatus
MDLVPLVTGQIKCIAYYTTGLVLASIVLNVIKQLVFYNRKEPPV
VFHWIPFIGSTVAYGMDPYQFFFASRAKYGNIFTFILLGKKTTVYLGVEGNEFILNGK
LKDVNAEEIYGKLTTPVFGSDVVYDCPNSKLMEQKKFIKYGLSQEALESYVPLIADEI
SSYIKSSPSFKGQSGTIDLVPAMAEITTFTAARTLQGEEVRSKLTTEFAKLFHDLDLG
FTPINFMLPWAPLPQNRKRDRAHRRMREIYVDIIQARREAGEEANDNGRDKTKGTDMI
SNLMRCVYRDGTPIPDKEIAHLMITLLMAGQHSSASISCWILLRLASQPEMTEKLFAE
QVNNLGADLPLLQYKDLDKLPLHRNVIKETLRLHSSIHTLMRKVKNPMPVPGTDFVIP
PSHTLLSSPGVTARDERHFRDPLRWDPHRWGSRVEAEDSSDTVDYRYGAVSKGTRSPY
LPFGAGRHRCIGEKFAYLNLGVIIATLLREFRFFNPEGMEGVPDTDYSSLFSRPMQPA
TVRWEVRS
CYP51F3 Fusarium graminearum
AACM01000457 (WGS section)
FG11024.1 FGcontig1.457_scaffold8
50% to 51F1 N.crassa (third CYP51 in this genome)
MESLYETLRTLPLSVSIPLTTSIIIILSIVTNVVKQLWFPNPHRPPVVFH
IFPFIGSTVQYGIDPYAFFFDCRDKYGDCFTFILLGKSTTVFLGPKGNDF
ILNGKHADLNAEDVYGKLTTPVFGEEVVYDCSNARFMDQKRLLKLGLTTD
SLRCYIPKFVKEVEDYVKNSPYFKGDTGIVNITEVMAEITIYTASGSLLG
NEVRSMFDSTFATLYRHLDDGFQPINFVMPGLPLPQNFRRNHARKVMEKL
FSDIISKRRETGNQGDETDMIWMLMNAQYKDGEPLPDHHAARMLIAILMG
GQHNTAVSGAWLLLNLAHKPHLVQELYEEQTQVLGSPQEPLTWENLQKLT
LNGQVIKETLRLHSPIHSILRQVKSPMRVPGTEWVVPPSHTLLSSPGTMA
RSEEFFPRPSEWDPHRWDKIEPLVKTAEDGQTVDYGFGVMSKSVSSPYLP
FGAGRHRCVGENYAYAQLGAIVATFIRLVHIEQPDPKAPLPAPDYSSMFS
RPMNPAEIRWRRRETVE*
CYP51F3 Fusarium oxysporum
FOXG_13138
87% to CYP51F3 Fusarium graminearum
note: gc boundary at DQKR
see fungal pages for seq
CYP51F3 Fusarium verticillioides
FVEG_12391
95% to CYP51F3 Fusarium oxysporum
see fungal pages for seq
CYP51F3 Nectria haematococca (Fusarium solani group)
fgenesh1_pg.scaffold_6000765
Necha1/scaffold_6:2267196-2268844
81% to Fusarium graminearum CYP51F3
52% to fgenesh1_pg.scaffold_2000426,
Note two exons, but the second exon is not seen in CYP51F1 or CYP51F2
MESLYGSFRALPLSISIPFTVGLVVVIAIARNVIGQLYFPDRNRPPVVFHVFPFIGSSVQYGIDPYKFFF
DCKAKYGDCFTFILLGKPTTVFLGQKGNEFILNGKHADLNAEEVYGKLTTPVFGKEVVYDCSNARFMDQKR (0)
LLKLGLTTEALRAYIPKFVKEVEDYVNTSPYFKGETGQVNITEVMAEITIYTASGSLLGNEVRSMFDST
FALLYRHLDDGFQPINFVMPWLPLPQNRRRDHARNVMEGLFANIIRQRREQGNQGDETDMIWTLMDAQYK
DGTVLPDIHVARLLIAILMGGQHNTAASGAWLFLNLAHKPHLVEEMYQEQLEVLGSPPAPLTWENVQKLT
LNGQVIKETLRLHSPIHSIMREVKSPMPVPGTEWVVPPSHTLLASPGTLARSEDFFPRPLEWDPHRWDKA
DPVEGPVDDSQKVDYGFGMMSKAVSSPYLPFGAGRHRCVGEHYAYAQLGAIVATFVRLCHMEQVDPKAPI
PATDYS (1)
SMFSRPMNPAVIKWRRRKPE*
CYP51F4 Aspergillus oryzae
GenEMBL BAE63554.1
72% to 51F2 Aspergillus nidulans, 77% to 51F2 Aspergillus oryzae
60% to 51F1 Aspergillus oryzae
MSWPRIGAYALLAFVAIMALNVTYQFLFRMLNKTRPPLVFHWIPFIGSTIHYGTDPYGFFFSCREKYGDI
FTFILLGRPTTVYLGTQGNEFILNGKLKDVNAEEVYSPLTTPVFGSDVVYDCPNSKLIEQKKFIKFGLSQ
AALEAHVPLIEKEVEDYLAMSPNFHGTSGEVDIPAAMAEITIFTAGSALQGEEVRSKLTTEFAVLYHDLD
KGFTPINFMLPWAPLPHNKKRDAAHARMRAIYIDIINKRRNAGDNVPEKLDMIGNLMQCTYKNGQPLPDK
EIAHIMITLLMAGQHSSSSISSWIMLRLASQPAVVEELYQEQLANLERTGPNGSLAPLQYKDFDNLPLHQ
NVIRETLRLHSSIHSLLRKVKNPLPVPGTPYVIPTSHVLLAAPGVTALSDEYFPNAMAWDPHRWETQAPQ
ENNKDDIVDYGYGAMSKGTSSPYLPFGAGRHRCIGEKFAYLNLAVIVATMVRHLRFSNLDGQTGVPDTDY
SSLFSGPMKPARIRWERRAAKSG
CYP51F5 Rhizopus oryzae (Zygomycete fungus, Mucoromycotina)
RO3G_16595.1
62% to CYP51F1 Rhizopus oryzae
42% to CYP51F1 Ustilago maydis in the CYP51 family
45% to CYP51F1 S. pombe
see fungal pages for seq
CYP51F6 Kluyveromyces polysporus
Kpol_1023p61
65% to CYP51F1 S. cerevisiae
see fungal pages for seq
CYP51F7 Uncinocarpus reesii
UREG_00593.1
58% to CYP51F Uncinocarpus reesii from PPVV on
62% to Aspergillus terreus CYP51F1
model is short. Seq. runs into a gap, missing last exon
see fungal pages for seq
CYP51F7 Coccidioides immitis
CIMG_00573.2
84% to CYP51F7 Uncinocarpus reesii
(ortholog to second CYP51 in this species)
CIMG_00573.2
CYP51G1 Triticum aestivum (wheat)
Francis Durst
CYP51BLE
submitted to nomenclature committee
note: CYP51G subfamily is for Green plants and their close relatives
see under plants for more 51G sequences
CYP51H1 Oryza sativa (rice)
note: the CYP51H subfamily is for non-typical CYP51s in Green plants
and their close relatives see under plants for more 51H sequences
52A Subfamily
CYP52A1 Candida tropicalis
GenEMBL M24894 M15945
Sanglard,D. and Loper,J.C.
Characterization of the alkane-inducible cytochrome P450 (P450alk)
gene from the yeast Candida tropicalis: identification of a new
P450 gene family.
Gene 76, 121-136 (1989)
CYP52A2 Candida tropicalis
Swiss P30607 (522 amino acids) GenEMBL M63258 (5745bp)
Seghezzi,W., Sanglard,D. and Fiechter,A.
Characterization of a second alkane-inducible cytochrome
P450p-encoding gene, CYP52A2, from Candida tropicalis
Gene 106, 51-60 (1991)
CYP52A3 Candida maltosa
GenEMBL D12475 D01168 (2047bp)
Ohkuma,M., Hijiki,T., Tanimoto,T., Schunck,W.H., Muller,H.G.,
Yano,K. and Takagi,M.
Evidence that more than one gene encodes n-alkane-inducible
cytochrome P-450s in Candida maltosa, found by two-step gene
disruption.
Agric. Biol. Chem. 55, 1757-1764 (1991)
Note: This is CYP52A3-b, one of two alleles in C. maltosa.
CYP52A3 Candida maltosa
GenEMBL S77461 (2047bp) Swiss P24458 (523 amino acids)
Ohkuma,M., Hikiji,T., Tanimoto,T., Schunck,W.H., Muller,H.G.,
Yano,K. and Takagi,M.
Evidence that more than one gene encodes n-alkane-inducible
cytochrome P-450s in Candida maltosa, found by two-step gene
disruption
Agric. Biol. Chem. 55, 1757-1764 (1991)
CYP52A4 Candida maltosa
GenEMBL X51932, S64322 (1787bp)
Schunck,W.H., Vogel,F., Gross,B., Kargel,E., Mauersberger,S.,
Kopke,K., Gengnagel,C. and Muller,H.G.
Comparison of two cytochromes P-450 from Candida maltosa:
primary structures, substrate specificities and effects of
their expression in Saccharomyces cerevisiae on the proliferation
of the endoplasmic reticulum.
Eur. J. Cell Biol. 55, 336-345 (1991)
CYP52A5 Candida maltosa
GenEMBL D12714
Ohkuma,M.
GenEMBL X55881
Ohkuma,M., Muraoka,S., Tanimoto,T., Fujii,M., Ohta,A. and Takagi,M.
CYP52 (cytochrome P450alk) multigene family in Candida maltosa:
identification and characterization of eight members.
DNA Cell Biol. 14, 163-173 (1995)
CYP52A6 Candida tropicalis
GenEMBL Z13010 (2501bp) Swiss P30608 (524 amino acids)
Seghezzi,W., Meili,C., Ruffiner,R., Kuenzi,R., Sanglard,D. and
Fiechter,A.
Identification and characterization of additional members of the
cytochrome P450 gene family CYP52 of Candida tropicalis.
DNA Cell Biol. 11, 767-780 (1992)
CYP52A7 Candida tropicalis
GenEMBL Z13011 (2087bp) Swiss P30609 (507 amino acids)
Seghezzi,W., Meili,C., Ruffiner,R., Kuenzi,R., Sanglard,D. and
Fiechter,A.
Identification and characterization of additional members of the
cytochrome P450 gene family CYP52 of Candida tropicalis.
DNA Cell Biol. 11, 767-780 (1992)
CYP52A8 Candida tropicalis
GenEMBL Z13012 (1929bp) Swiss P30610 (517 amino acids)
Seghezzi,W., Meili,C., Ruffiner,R., Kuenzi,R., Sanglard,D. and
Fiechter,A.
Identification and characterization of additional members of the
cytochrome P450 gene family CYP52 of Candida tropicalis.
DNA Cell Biol. 11, 767-780 (1992)
CYP52A9 Candida maltosa
GenEMBL D26160 (283bp)
Ohkuma,M.
unpublished
ALK5-B allele of CYP52A9 ALK5-A
CYP52A9 Candida maltosa
PIR JS0723
ALK5-A
CYP52A10 Candida maltosa
CYP52A11 Candida maltosa
GenEMBL D26159 (815bp)
Ohkuma,M.
unpublished
ALK8-B allele of CYP52A11 ALK8-A
Note on CYP52A12-CYP52A20 and CYP52D2 from Candida tropicalis.
These sequences seem to be orthologs of other Candida tropicalis sequences.
52A12 seems to be an ortholog of 52A6
52A13 and 52A14 seem to be orthologs of 52A2
52A15 and 52A16 seem to be orthologs of 52A1
52A17 and 52A18 seem top be orthologs of 52A8
52A19 and 52A20 seem to be orthologs of 52A7
the last 8 listed appear in what might be allelic pairs 94-96% identical to each other.
It seems probable that these actually are the orthologs of those other sequences
and this Candida tropicalis strain is different from the other.
CYP52D2 matches Candida maltosa 52D1 and was not observed in C. tropicalis before.
CYP52A12 Candida tropicalis
No accession number
David Craft Cognis Corp.
Submitted to nomenclature committee June 26, 2001
Clone name 1p
90% to CYP52A6
CYP52A13 Candida tropicalis
No accession number
David Craft Cognis Corp.
Submitted to nomenclature committee June 26, 2001
Clone name 2p might be an allele of CYP52A14 (96% identical)
86% to CYP52A2
CYP52A14 Candida tropicalis
No accession number
David Craft Cognis Corp.
Submitted to nomenclature committee June 26, 2001
Clone name 3p might be an allele of CYP52A13 (96% identical)
87% to CYP52A2
CYP52A15 Candida tropicalis
No accession number
David Craft Cognis Corp.
Submitted to nomenclature committee June 26, 2001
Clone name 4p might be an allele of CYP52A16 (95% identical)
85% to CYP52A1
CYP52A16 Candida tropicalis
No accession number
David Craft Cognis Corp.
Submitted to nomenclature committee June 26, 2001
Clone name 5p might be an allele of CYP52A15 (95% identical)
84% to CYP52A1
CYP52A17 Candida tropicalis
No accession number
David Craft Cognis Corp.
Submitted to nomenclature committee June 26, 2001
Clone name 7p might be an allele of CYP52A18 (94% identical)
78% to CYP52A8
CYP52A18 Candida tropicalis
No accession number
David Craft Cognis Corp.
Submitted to nomenclature committee June 26, 2001
Clone name 8p might be an allele of CYP52A17 (94% identical)
77% to CYP52A8
CYP52A19 Candida tropicalis
No accession number
David Craft Cognis Corp.
Submitted to nomenclature committee June 26, 2001
Clone name 9p might be an allele of CYP52A20 (95% identical)
75% to CYP52A7
CYP52A20 Candida tropicalis
No accession number
David Craft Cognis Corp.
Submitted to nomenclature committee June 26, 2001
Clone name 10p might be an allele of CYP52A19 (95% identical)
76% to CYP52A7
CYP52A21 Candida albicans
GenEMBL XM_705082 (one aa diff to the Kim seq), orf19_10
XM_705076 (one aa diff to the Kim seq)
Y14766 (3 aa diffs to the Kim seq)
Donghak Kim and Paul Ortiz de Montellano
Submitted to nomenclature committee July 21, 2006
72% to CYP52A11 (ALK8) of Candida maltosa
CYP52A22 Candida albicans SC5314
79% to CYP52A4 orf19_7512
see Fungal pages for seq
CYP52A23 Candida albicans SC5314
78% to CYP52A5 orf19_7513
see Fungal pages for seq
CYP52A24 Candida albicans SC5314
78% to CYP52A3 orf19_5728 partial sequence
see Fungal pages for seq
CYP52A25 Candida dublinensis
cdub_593-g99.1
79% to CYP52A3 Candida maltosa
see Fungal pages for seq
CYP52A26 Candida dublinensis
cdub_14-g136.1
89% to CYP52A21 Candida albicans
see Fungal pages for seq
CYP52A27 Candida dublinensis
cdub_14-g720.1
94% to CYP52A23 Candida albicans
see Fungal pages for seq
CYP52A28 Candida dublinensis
cdub_14-g719.1
93% to CYP52A22 Candida albicans
see Fungal pages for seq
CYP52A29 Candida lusitaniae
CLUG_03984.1
55% to CYP52A13 Candida tropicalis
see Fungal pages for seq
CYP52A30 Candida lusitaniae
CLUG_04851.1
55% to CYP52A23 Candida albicans
see Fungal pages for seq
CYP52A31 Candida lusitaniae
CLUG_04098.1
52% to CYP52A18 Candida tropicalis
see Fungal pages for seq
CYP52A32 Candida parapsilosis
CPAG_03577
71% to CYP52A5 Candida maltosa
see Fungal pages for seq
CYP52A33 Candida parapsilosis
CPAG_04394
73% to CYP52A5 Candida maltosa
see Fungal pages for seq
CYP52A34 Candida parapsilosis
CPAG_04395
71% to CYP52A5 Candida maltosa
see Fungal pages for seq
CYP52A35 Candida parapsilosis
CPAG_05481
65% to CYP52A21 Candida albicans
see Fungal pages for seq
CYP52A36 Candida parapsilosis
CPAG_05482
65% to CYP52A21 Candida albicans
see Fungal pages for seq
CYP52A37 Candida parapsilosis
CPAG_05523
58% to CYP52A12 Candida tropicalis
see Fungal pages for seq
CYP52A38 Candida parapsilosis
CPAG_02285
74% to CYP52A25 Candida dublinensis
see Fungal pages for seq
CYP52A39 Candida guilliermondii
PGUG_01238.1
66% to CYP52A14 Candida tropicalis
see Fungal pages for seq
CYP52A40 Candida guilliermondii
PGUG_05855.1
60% to CYP52A25 Candida dublinensis
see Fungal pages for seq
CYP52A41 Candida guilliermondii
PGUG_04005.1
52% to CYP52A12 Candida tropicalis
see Fungal pages for seq
CYP52A42 Candida guilliermondii
PGUG_05670.1
62% to CYP52A23 Candida albicans
see Fungal pages for seq
CYP52A43 Debaryomyces hansenii
CAG85755.1
54% to CYP52A3 Candida maltosa
see Fungal pages for seq
CYP52A44 Debaryomyces hansenii
CAG85832.1
63% to CYP52A25 Candida dublinensis
see Fungal pages for seq
CYP52A45 Debaryomyces hansenii
CAG88380.1
62% to CYP52A14 Candida tropicalis
see Fungal pages for seq
CYP52A46 Debaryomyces hansenii
CAG88381.1
63% to CYP52A14 Candida tropicalis
see Fungal pages for seq
CYP52A47 Debaryomyces hansenii
CAG88382.1
65% to CYP52A14 Candida tropicalis
see Fungal pages for seq
CYP52A48 Lodderomyces elongisporus
LELG_04957
73% to CYP52A38 Candida parapsilosis
see Fungal pages for seq
CYP52A49 Lodderomyces elongisporus
LELG_03506
72% to CYP52A37 Candida parapsilosis
see Fungal pages for seq
CYP52A50 Lodderomyces elongisporus
LELG_03505
71% to CYP52A37 Candida parapsilosis
see Fungal pages for seq
CYP52A51 Lodderomyces elongisporus
LELG_03309
75% to CYP52A35 Candida parapsilosis
see Fungal pages for seq
CYP52A52 Lodderomyces elongisporus
LELG_00044
70% to CYP52A5 Candida maltosa
see Fungal pages for seq
CYP52A53 Pichia stipitis
JGI model e_gww1.3.1.449.1
65% to CYP52A40 Candida guilliermondii
see Fungal pages for seq
CYP52A54 Pichia stipitis
JGI model e_gww1.3.1.459.1
63% to CYP52A44 Debaryomyces hansenii
see Fungal pages for seq
CYP52A55 Pichia stipitis
JGI model fgenesh1_pm.C_chr_3.1000237
63% to CYP52A14 Candida tropicalis
see Fungal pages for seq
CYP52A56 Pichia stipitis
JGI model fgenesh1_pm.C_chr_8.1000042
59% to CYP52A38 Candida parapsilosis
see Fungal pages for seq
CYP52A57 Pichia stipitis
JGI model e_gww1.3.1.1392.1
62% to CYP52A39 Candida guilliermondii
see Fungal pages for seq
52B Subfamily
CYP52B1 Candida tropicalis
GenEMBL Z13013 (2442bp) Swiss P30611 (506 amino acids)
Seghezzi,W., Meili,C., Ruffiner,R., Kuenzi,R., Sanglard,D. and
Fiechter,A.
Identification and characterization of additional members of the
cytochrome P450 gene family CYP52 of Candida tropicalis.
DNA Cell Biol. 11, 767-780 (1992)
52C Subfamily
CYP52C1 Candida tropicalis
GenEMBL Z13014 (1622bp) Swiss P30612 (505 amino acids)
Seghezzi,W., Meili,C., Ruffiner,R., Kuenzi,R., Sanglard,D. and
Fiechter,A.
Identification and characterization of additional members of the
cytochrome P450 gene family CYP52 of Candida tropicalis.
DNA Cell Biol. 11, 767-780 (1992)
CYP52C2 Candida maltosa
D12718
CYP52C3 Candida albicans SC5314
60% to CYP52C2 orf19_6574
see Fungal pages for seq
CYP52C4 Candida dublinensis
cdub_588-g100.1
94% to CYP52C3 Candida albicans (ortholog)
see Fungal pages for seq
CYP52C5 Candida parapsilosis
CPAG_01250
48% to CYP52C3 Candida albican
There is one 52C seq per Candida species except this
species has two. They all seem to be orthologs even
though the percent identity is not high
see Fungal pages for seq
CYP52C6 Candida parapsilosis
CPAG_01665
50% to CYP52C2 Candida maltosa
53% to to CYP52C5 this species
see Fungal pages for seq
CYP52C7 Lodderomyces elongisporus
LELG_05768
54% to CYP52C6 Candida parapsilosis
48% to CYP52C3 Candida albicans
see Fungal pages for seq
52D Subfamily
CYP52D1 Candida maltosa
CYP52D2 Candida tropicalis
No accession number
David Craft Cognis Corp.
Submitted to nomenclature committee June 26, 2001
Clone name 6p
61% to CYP52D1
52E Subfamily
CYP52E1 Candida apicola
GenEMBL X76225 (1560bp) PIR S38894 (519 amino acids)
Lottermoser,K.
unpublished (1994)
CYP52E2 Candida apicola
GenEMBL X87640 (2203bp)
Lottermoser,K., Schunck,W.H. and Asperger,O.
Cytochromes P450 of the sophorose lipid-producing yeast Candida
apicola: heterogeneity and polymerase chain reaction-mediated
cloning of two genes
Yeast 12 (6), 565-575 (1996)
CYP52E3 Candida bombicola, alternative name Starmerella bombicola
EU552420
Inge Van Bogaert
Submitted to nomenclature committee Dec. 7, 2007
Gene2
92% to 52E2, 82% to 52E1
52F Subfamily
CYP52F1 Yarrowia lipolytica
GenEMBL AB010388 (3578bp), GenPept CAG80007.1
Iida,T., Ohta,A. and Takagi,M.
Cloning and characterization of an n-alkane-inducible cytochrome
P450 gene essential for n-decane assimilation by Yarrowia
lipolytica.
Yeast 14 (15), 1387-1397 (1998)
gene for ALK1
see Fungal pages for seq
CYP52F2 Yarrowia lipolytica gene for ALK2
GenEMBL AB010389, GenPept CAG77659.1
Iida,T., Ohta,A. and Takagi,M.
Yarrowia lipolytica cytochrome P450 gene, complete cds
unpublished
see Fungal pages for seq
CYP52F3 Yarrowia lipolytica gene for ALK3
GenEMBL AB010390, GenPept CAG79910.1
Iida,T., Ohta,A. and Takagi,M.
Yarrowia lipolytica cytochrome P450 gene, complete cds
unpublished
see Fungal pages for seq
CYP52F4 Yarrowia lipolytica gene for ALK4
GenEMBL AB010391, GenPept CAG83106.1
Iida,T., Ohta,A. and Takagi,M.
Yarrowia lipolytica cytochrome P450 gene, complete cds
unpublished
see Fungal pages for seq
CYP52F5 Yarrowia lipolytica gene for ALK5
GenEMBL AB010392, GenPept CAG83107.1
Iida,T., Ohta,A. and Takagi,M.
Yarrowia lipolytica cytochrome P450 gene, complete cds
unpublished
see Fungal pages for seq
CYP52F6 Yarrowia lipolytica gene for ALK6
GenEMBL AB010393, GenPept CAG82620.1
Iida,T., Ohta,A. and Takagi,M.
Yarrowia lipolytica cytochrome P450 gene, complete cds
unpublished
see Fungal pages for seq
CYP52F7 Yarrowia lipolytica gene for ALK7
GenEMBL AB010394, GenPept CAG84028.1
Iida,T., Ohta,A. and Takagi,M.
Yarrowia lipolytica cytochrome P450 gene, complete cds
unpublished
see Fungal pages for seq
CYP52F8 Yarrowia lipolytica gene for ALK8
GenEMBL AB010395, GenPept CAG82058.1
Iida,T., Ohta,A. and Takagi,M.
Yarrowia lipolytica cytochrome P450 gene, complete cds
unpublished
see Fungal pages for seq
CYP52F9 Yarrowia lipolytica
CAG84211.1
64% to CYP52F3
see Fungal pages for seq
CYP52F10 Yarrowia lipolytica
CAG82791.1
71% to CYP52F1
see Fungal pages for seq
CYP52F11 Yarrowia lipolytica
CAG83401.1
69% to CYP52F2
see Fungal pages for seq
CYP52G1 Aspergillus nidulans
AN6057.1
38% to 539A1 46% to 52F1 52 clan
see Fungal pages for seq
CYP52G2 Aspergillus fumigatus Af293
GenEMBL XP_755288.1 also EAL93250.1
60% to 52G1
MGLPLLTIAAVAVALRLLWTLAARWQHAQNARRLGCGSIPLYPSDPLGISVLKETLAADKAKKLLPLVER
RVALMSDREGRYVTTFRFRQMGRENYFTTDPKNIQAILATQFKDFELGAPRRQALHPLLGAGIFSSDGEE
WFHARGLLRPQFTRDQISDLDLEERHVQKAMQAMPVVNGKWTDAVDIQSIFFRLTIDSATEFLFGESVES
QLSALNGGQTPVDTFPYYFDKSQWYAAQRARFEKLYWIVNNKECRHAQNEVHAYVDRVVHKALKAAREGK
LANPNKPSQYVFLHALVSVTQDPIELRSQLLNILLAGRDTTASLLSWTVLMLARHPAEFHKLRQTIVDEF
GTYDQPRNITFAALKSCQYLQYCLNETLRLFPVVPGNRRSATRDTTLPRGGGSDGTQPIYIRKGQTVVYN
VHILHRRKDIWGPDAEEFKPSRWVDRKVGWDYVPFNGGPRICIGQQFALTEAGYVLVRLLQRFDAIEDMQ
PHLEIRHSLNLTSAPADNVTVRLREAA
CYP52G2 Neosartorya fischeri
NFIA_085030
97% to CYP52G2 Aspergillus fumigatus = ortholog
see Fungal pages for seq
CYP52G3 Aspergillus oryzae
GenEMBL BAE65166.1, AP007171.1
58% to CYP52G4, 63% to 52G1
14 P450 genes and 2 pseudogenes on this contig
MIPLLILAALAVVFRLVWSIFTSLRHAQNARKWHCGAIPTYPGDILGINTLKEVLRADKEKLIPVLSAQR
VETMTAREGRYVSTFRLRQMGRESIFTSDPKNMQAILATQFKEFELGSLRRNSLHPLLGSGIFSTDGEAW
SRSRSLLRPQFTRDQVSDLDLEERHVQKAMAGMLADPATKWTPEIDIQSIFFRLTIDSATEFLFGDSVES
QTAALSGSRIIEDKFPSYFDRGQWYAAQRARFEKLYWIVNNKESRETDRFVHAYVDRFIDAALAAVKEGK
IDPEKRNSDHYVFLHGLTTATQDPVELRSQLLNILLAGRDTTASLLSWCVLLLARHPDIFQKLRNTILAD
FGDYRNPRNITFSSLKSCRYLHYFMNEVLRLYPIVPGNRRVALKDTTLPRGGGPDGSEPVYVRKGQPVVY
SVFVTHRRKDIWGADAEVFNPDRWEDLKVGWEYLPFNGGPRICIGQQFALTEAGYVLVRLLQRFDQIVDA
RPEREIRFNATLTSAPWENVIVRLREGA
CYP52G3 Aspergillus flavus
AFL2G_05445
99% to CYP52G3 Aspergillus oryzae
see Fungal pages for seq
CYP52G4 Aspergillus oryzae
GenEMBL BAE55312.1
54% to 52G1
MSDARTTLMETWFTPALVAAIVAARLLFSLYSAWRHAQRARSLNCQEAPLYPSRDPFGVATLLETLRADR
DKFLPKLSQKRVDLISSQQNRYVSTFRVRQAGRENFFTVDPKNIQAMLATQFNDFFLGDMRRNAGAPVIR
SGIFVSDGADWSHSRSLIRPQFTRTQINNLELEERHVQNALRAMPTQSNGWTSEVDIQTILFRLTLDSAT
EFLFGKSCNSQLTALEKDAGEMSDSFLNSFDRCAWYLAARLRFERLYWIVNNKEFRECTRVVHELVDGYV
HAALQRAQQAEKPADPERGSHYVFVDALTATTQDPNQLRDECLNVLIAGCDTTASLLSWNILLLARHPDI
FQRLRKEIIERFGTYSEPRDINFSSLKSCQYLQHFINETLRLHPVVPFNRRCANKDTTLPRGGGKDGNSP
VYLQKGQPVLYSSYVLQRRKDIWGEDAEEFNPDRWYGRKAIWEHIPFSGGPRTCIGQQFAITNTSFVLVR
LLQRFDSIEDVYPEREIRYGVTLTNCPADRVTVRMHQAEL
CYP52G4 Aspergillus flavus
99% to CYP52G4 Aspergillus oryzae
AFL2G_00228
see Fungal pages for seq
CYP52G5P Nectria haematococca
e_gw1.8.757.1
Necha1/scaffold_8:999970-1000262
old name = CYP-un1 pseudogene
PSEUDOGENE very short piece
57% to 538A3, 54% to 584D1, 61% to 52A3, 68% to 52G2
DESLRLYPVEPINARYANKDTTLPRGGGKDGNSPILIPKGSSTAFSVHIIHRRKDIRGPD
ANEFKPERWEGRRVGWEYVP ()
FNGGPRICIG**FALIGASYVTVRLLQRFDKMESLEKDAVVRHNLKLINYVANGVKVRLHAAS
CYP52G6 Aspergillus niger
fgenesh1_pg.C_scaffold_15000225|Aspni1
57% to CYP52G2
see Fungal pages for seq
CYP52G7 Aspergillus niger
e_gw1.7.657.1|Aspni1
67% to CYP52G3
see Fungal pages for seq
CYP52G8 Aspergillus clavatus
ACLA_081330
83% to CYP52G2
see Fungal pages for seq
CYP52G9 Aspergillus terreus NIH2624
GenPept EAU33222.1
ATEG_06678
CH476602
complement(join(1144551..1145711,1145779..1146177))
62% to CYP52G2
see Fungal pages for seq
CYP52G10 Aspergillus terreus
ATEG_07540.1
68% to CYP52G4
see Fungal pages for seq
CYP52H1 Aspergillus nidulans
AN7131.1
45% to 584B1 52 clan
see Fungal pages for seq
CYP52H2 Aspergillus fumigatus Af293
GenEMBL XP_746567.1 also EAL84529.1
cytochrome P450 alkane hydroxylase
72% to 52H1
MLAPAVALFAAVYTTFLVLRYFQQLYKHRKQARSLRCQPPADGEAGFLGIASFLRLRKAAKEKRWIELIA
EQYGKYGNTFTQTIFGQPLVSTIEPENLKALLATQFNDFELGTRHREFYPLLGDGIFTLDGAGWSHARAL
LRPQFTRDQVADLDLMDGHVSRLIDLVPKDGSAFDIQRLFFLLTIDSATHFLFGESVGSLHAGTDTGLLG
RSAVGNAEGFAEAFNKAQEYLAARSRAVVFYWMINPKEFRDANKRVHEVVDHYVQLALESRRNPEKKQPG
GRYIFAEALAAENDNPKVLRDNMLNILLAGRDTTASLLSSTFFYLARHPNVWIKLRQVIVDAFGDAEHPK
EEITQTKLKDIPYLRYVLNEVLRLLPPVPANFRVANKDTTLPVGGGPDQKSPVYIRKGTIVTYSVYAMHR
RTDFYGPDANEFRPERWEENGKRGWEYLPFNGGPRICLGQQYALTEASFTIVKLLQRFDRIENGDPDLIE
PIQQTNLTLAHDRGVYIRLYSSKAL
CYP52H2 Neosartorya fischeri
NFIA_029600
95% to CYP52H2 Aspergillus fumigatus = ortholog
see Fungal pages for seq
CYP52H3 Aspergillus oryzae
GenEMBL BAE64862.1, AP007171.1
71% to 52H1, 48% to 584G1
14 P450 genes and 2 pseudogenes on this contig
MLAQIAPLFGALCITFLVLQYLQRLYQQRKRAQSLGCQPAAQGPSGIFGIRSFVRLLNEVRHKRWVEYIA
GQYGRYGNTYTQKALGKWMVSTIEPENIKALLATQFNDFGLGTRHREFYPLLGDGIFTLDGPGWSHARGL
LRPQFTRDQVADLELMDGHISRMIDLIPQDGSSFDIQRLFFLLTIDSATHFLFGESVGALESSNSASLLG
RSSVGSAQGFAEAFGTAQDYLTTRSRAMHFYWMVNPKEFREANQRVHEVVDHYVQLAIQSKNNPDKKSDR
YIFAEALAADNDDPKVLRDNMLNILVAGRDTTASLLSSAFFYLSRNQDVWKKLRQTIIDEFGDSQNPKGE
ITQAKLKDIPYLRYVLNEVLRLLPPVPLNFRVAAKDTSLPVGGGPDGRSPVFIPKGQVVAYSVYAMHRRT
DLYGPDSHSFRPERWEENGRRGWDYLPFNGGPRICLGQQYALTEASYTLVKLVQRFDTLECADPELKQPA
ILSTLTMSHDRGVKVRLSSSVPKCWFNQ
CYP52H3 Aspergillus flavus
AFL2G_05134
100% to CYP52H3 Aspergillus oryzae
see Fungal pages for seq
CYP52H4 Aspergillus niger
estExt_GeneWisePlus.C_80246|Aspni1
73% to CYP52H2
see Fungal pages for seq
CYP52H5 Aspergillus clavatus
ACLA_054640
84% to CYP52H2 Aspergillus fumigatus
see Fungal pages for seq
CYP52H6 Aspergillus terreus
ATEG_02198.1
75% to CYP52H5
see Fungal pages for seq
CYP52J1P Aspergillus fumigatus Af293
GenEMBL XP_746816.1 also EAL84778.1
alkane hydroxylase predicted
42% to CYP52F1, in 52 clan
insertion of 11 C bases causes frameshift = &
MTNTDIAFGHQHGCELPPELTKNWPLGLDRIKELWTANTEGHLLAFLCSVAEKYEPGNSITQYFLFGPRA
FHILQPESVEAILSTNFKDYGFGARAAIFAPRLGNGIFTQEGPAWRHSRDLLRKQFSRVQNRILEHFHEH
VDNMVARLPLDGVVDLQPLFFNLTLDIATALLFGRSVYSLLAGIDQDADNGLFAEGFNIAQDGLAKRFRI
APWHFLYNPPGFRKACGDVHRFVEQYIDQLDLENSEDLDDKTYGFIKRVAHESASRQHLRDQLLNVLLAG
RDTTACCLSWTFRLLVHHEQAMIRLREEIASVMGDSAHPTKEQIRKMPYLSCVIKE
SLRLYPPP &
PPVPLNNREAIRTTILPTGGGPDADRPILVRKGELVVFFQYVNSRKKNIYGPDADCFRP
ERWETGELDHIGWAYFPFNGGPRQCLGENFALMEVSYTVVRLLQTFSSIILPKGQPIEPVGSERQRLTLV
LSSADGCKVQIHS
CYP52J1 Neosartorya fischeri
NFIA_113870
95% to CYP52J1 Aspergillus fumigatus = ortholog
see Fungal pages for seq
CYP52J2 Uncinocarpus reesii
UREG_00942.1
71% to 52J1 in overlapping parts
see Fungal pages for seq
CYP52K1 Aspergillus oryzae
GenEMBL BAE66393.1
44% to 52A5, 44% TO CYP52J1
MLTLSFSALLGIALAWAIHRLAWKYSRLKAICVKRDNKLATQYGCEQPPRLRNWWPLGIDRLIQIWTADS
EQRLMDLFTFPFKDVGYTLEQKFLGTIAFGTIDPENLEAIMTNINVFSFGLRRHILFPLLGDGIFTQEGK
SWRHSRELLRPQFTRQHYRDLNIFRPHVDRLLHYLTGNGAATDLQPLFFRLTLDTTTEYLFGKSVNSLMP
GEATKGQSFANHFDMAQNYVVQRFRLLDLYWLIGGPKFWRSCSAVHRFIDEIIDTRMDSKEKDKDCNSKS
IFFDAVARDSRTRRDLRDQLTNVLLAGRDTTACLLSWTFHCLARHPDVLGRLNVEIASVVGTNTDLTRDD
FKRMPYLDQVLREVLRLYPPVPVNTRTAHKTTILPTGGGKDGTKPFMVREGENVAFCVYAMHRREDLYGP
DAGQFRPERWDEDLPLFQNERTATWGYLPFNGGPRACLGQDFGFVEAAYTVVRILQKYPIVKPDMYKGDI
QGRKWLGWSSHQPEGIEMVTEERQKMTIVLSLGDGCRVTLGR
CYP52K1P Aspergillus flavus
AFL2G_11704
97% to CYP52K1 Aspergillus oryzae
small 6 aa deletion and frameshift = &
see Fungal pages for seq
CYP52L1 Graphium sp. ATCC 58400
GenEMBL AY438638.1
Skinner,K.M., Cone,M. and Ciuffetti,L.M.
Characterization and cloning of an alkane inducible cytochrome P450
monooxygenase from a filamentous fungi, Graphium sp.
Unpublished
alkane monooxygenase P-450 (ALK1) gene
41/65 top blast hits to a complete set of named fungal P450s
are CYP52 sequences. The others are from CYP538, CYP539, CYP584, CYP585
related families in the CYP52 clan of fungal P450s.
45% to CYP52G2 Aspergillus fumigatus
MMDPFYLLGGCCVFGIVYKSVATLYVNYVHGKTARELGCKPLPK
ERTRFFGLDILRREMKADREMYLPSDIMTRFEEVGATTYEYQMLNEKHIATCDPLVIQ
TVLATQFKEFTFGNRPWGTVLGNGIFSIDNIGDNKEWLHTRAMLKPQFLRNQVSDLEL
EEGHVRNFMSVLKVGDDGWTPKVNLQHMFFNFTLDSATEFLFGKSADCQLLFAPGGDP
KGVSKPLRAFLKALDVAKETVNTKWFLGNNGWIADSPTFRKNCAYVNDFMDALIAKTK
AELEAKPKGSKPSGGREKYHFLHAMLDETDDKVELRGQALNILLAGRETTASLLGWLW
YYLARHPIEFQKLRAAVLKDFGTAENPKPMSFESLKACDQLQYCNNEILRLFPLVSYN
SRMALTDTTLPRGGGPDGNSPIFVKKGQQIVYHPHAMHRRKDIWGQDADEFRPDRWKT
LRPGWEYIPFNGGGRICMGQQFALTEASYLTVRLLQRFDRIENLDPNPVLKQKFRIVN
TPGEGVLVRLHEAAAGA
CYP52M1 Candida bombicola alternative name Starmerella bombicola
EU552419
Inge Van Bogaert
Submitted to nomenclature committee Dec. 7, 2007
gene1
45% to CYP52A3, 45% to CYP52E1
CYP52N1 Candida bombicola alternative name Starmerella bombicola
EU552421
Inge Van Bogaert
Submitted to nomenclature committee Dec. 7, 2007
Gene3
45% to CYP52M1, 45% to CYP52A15
CYP52P1 Aspergillus niger
gw1.4.1682.1|Aspni1
46% to CYP52K1
see Fungal pages for seq
CYP52P2 Mycosphaerella graminicola
Mgr076
53% to CYP52P1 A. niger, 47% to 52K1 (missing N-term)
yellow from JGI model e_gw1.8.895.1|Mycgr3
see Fungal pages for seq
CYP52Q1 Aspergillus niger
e_gw1.11.186.1|Aspni1
45% to CYP52H3, 41% to CYP52P1
see Fungal pages for seq
CYP52R1 Mycosphaerella graminicola
Mgr006
51% to CYP52G2 Aspergillus fumigatus
see Fungal pages for seq
CYP52R2 Mycosphaerella fijiensis
fgenesh1_pm.C_scaffold_45000005
60% to CYP52R1 Mycosphaerella graminicola
see Fungal pages for seq
CYP52S1 Yarrowia lipolytica
CAG81974.1
42% to 52H3, 44% to 52F1
see Fungal pages for seq
CYP52T1 Aspergillus terreus
ATEG_03903.1
48% to CYP52G2
see Fungal pages for seq
53A Subfamily
CYP53A1 Aspergillus niger
GenEMBL X52521
van Gorcom,R.F., Boschloo,J.G., Kuijvenhoven,A., Lange,J., van
Vark,A.J., Bos,C.J., van Balken,J.A., Pouwels,P.H. and van den
Hondel,C.A.
Isolation and molecular characterisation of the
benzoate-para-hydroxylase gene (bphA) of Aspergillus niger: a
member of a new gene family of the cytochrome P450 superfamily
Mol. Gen. Genet. 223, 192-197 (1990)
CYP53A2 Aspergillus parasiticus
GenEMBL AC005991
Lewis,J., Kupfer,D., Keller,N. and Roe,B.A.
Aspergillus parasiticus Cosmid Clone ap0
Unpublished
57% to CYP53A3
27896 MLLDLYLVKCLFCPL
27941 VPIVALGLLVAYYVAGYLKQWHLHDLPGPFIAGFSRIWLILQVRQGYRSLVVNDLHRRCG 28120
28121 KIVRLAPNHISVADESAIQAIYGHGNGFLK 28210 intron
28267 SNFYNAFLNVDWSIFTTRSRAEHTRKRKIVSHAFSARSLAQVEQYAHNTMELLVRQWQ 28440
28441 KMVDSQEGLDDPYAVIDARVWCNYLTFDIIGDLAFGAPFGMLERGNAIVSMRKALEDAAV 28620
28621 TLDAVEVLNHRGDVSAALGICPDLIPYAKWLPDLFFRQGAEAIANVADVAGAAVDRRLKM 28800
28801 NTSMTEKRGDLLAHLIDAEDQAGAKLGYRELTGEAVTLIAAGSDTSSSTLCALLYWVSTT 28980
28981 PRVLWKLQNVLDEVIPVDVEVPYLAMVKKI 29070 intron
29117 PSLQWVIWEALRIHSTFGQGLPREVPPERGPVEICSHTFYPGDVLSVPGYTMHHSVDIWG 29296
29297 IDVEDFVPERWDPCRLTQRQKDSFIPFSEGPRACIGRNLAEMEFFVGCATLFRLFEFRVE 29476
29477 GQGPLEVREGWLRKPVSLQVGIRRRYLDVRSS* 29575
CYP53A3 Aspergillus nidulans or Emericella nidulans
GenEMBL AY048583, AN7589.1
Fraser,J.A., Davis,M.A. and Hynes,M.J.
Submitted to nomenclature committee Oct. 3, 2000
benzoate-para-hydroxylase (bzuA) gene
82% identical to CYP53A1
MITDFLTPENITPERIALALLGLLAAYYVVPYLQTWRLSDIPAP
GLAAWTNFWLLLQTRLGHRFISVDNAHKKYGKLVRIAPRHISIADDAAIQAVYGHGNG
FLKSDFYDAFVSIRRGLFNTRDRAEHTRKRKTVSHTFSAKSIGQFEQYIHHNIENLVK
QLTRISNLQRNPKNGYATVDALNWFNFVAFDIIGDLAFGAPFGMLDKGQDIAEMRKSP
DSPPQYVQAVEVLNRRGEVSATLGCYPALKPFAKYLPDRFFRDGLEAVENLAGIAIAC
VNERLKPEVMANNTRVDLLARLMEGKDANGNKLGRAELTAEALTQLIAGSDTTSNTSC
AILYYCLRTPGVIDKLHKVLDEAIPQDVEVPTHAMVKEIPYLQWVIWETMRIHSTSAM
GLPREIPEGNPPVEISGHIFKPGDILSVPTYTIHHSKEIWGADADEFIPERWAPERLT
ARQKAAFIPFSTGPRACVGRNVAEMELLVICSTVFRMFDWELQQKGPMETREGFLRKP
LGLTVGVKRRTIV
CYP53A4 Neurospora crassa
AABX01000266.1 cont3.41 NCU01086.1 (version3)
9a61.tfa_50cg@9A61
65% to 53A1 over 417 aa
note the automated assembly of the gene is missing the KYG
fragment seen in 53A1. See my assembly for corrected seq.
Neurospora crassa sequence contig 1.248 (supercontig 26)
14619 MAIISLLMSPWAPVVLLAGVAFYYLVPYFVTYSALRKIPSPFPAQFTDLWLL
SVCRRGNRYQRVDELHKKLGPVVRIQPNHVSICDDAAIPTIYGHGNGF
LNDFYDAFVSIRRGLFNTRDRAEHTRKRKIVSHTFSAKSVQQFEPYMHSNLELFVKQWDSMIKNSKNPDKAAHLD
CLEWFNYLAFDVIGDLSFGQPFGMLSSGADMAEIRSSPDAAPIYAPAIEILNRRGEVSATLGIHPALKPFAKYLP
DPFFTKGLAAVENLAGIAIACVKSRLDNPPPVTRKDLLQRLMEGRDEKGEPLGREELTAEALTQLIAGSDTTSNS
SCALLFHAVRTPGVMQKLQAELDANIPPEVDVPTYDMVKELPYLEAVINEVLRFHSTSGIGLPRQIPHDASQGVH
IQGYYLPPGTVLSVPTYSIHHSKEIWGPDADEFKPERWERLTARQKNAFIPFSHGPRSCVGRNVAEMEMKLIVAT
WARRYEVKLLQDYMDTREGFLRKPLGLKVGLKLRK 13029
CYP53A4 Neurospora discreta
JGI gene model estExt_Genewise1Plus.C_10226
97% to CYP53A4 N. crassa
see Fungal pages for seq
CYP53A5 Magnaporthe grisea
MG07977.4
AACU01000984.1 cont2.1481 runs off end, changed C-term
AACU01000983 cont2.1480 = N-term
69% to CYP53A4 (split on two contigs)
see Fungal pages for seq
CYP53A6 Fusarium graminearum
FG08079.1 AACM01000324 FGcontig1.324_scaffold5
see Fungal pages for seq
CYP53A7 Fusarium graminearum
FG08086.1 AACM01000324 FGcontig1.324_scaffold5
see Fungal pages for seq
CYP53A8 Fusarium graminearum
FG10451.1 AACM01000435 FGcontig1.435_scaffold7
see Fungal pages for seq
CYP53A10 Nectria haematococca (Fusarium solani group)
83% to Fusarium graminearum CYP53A7 possible ortholog
fgenesh1_pg.scaffold_37000003
Necha1/scaffold_37:6771-8451
59% to fgenesh1_pg.scaffold_12000228,
Note: this seq has two more introns than the CYP53A8 ortholog
This gene model seems correct DRN 2/4/06
see Fungal pages for seq
CYP53A11 Nectria haematococca (Fusarium solani group)
fgenesh1_pg.scaffold_12000228
Necha1/scaffold_12:587439-589032
84% to Fusarium graminearum CYP53A8 possible ortholog
64% TO 53A1 Aspergillus niger
This gene model seems correct DRN 2/4/06
see Fungal pages for seq
CYP53A12 Aspergillus fumigatus Af293
GenEMBL XP_755858.1 also EAL93820.1
join(<2165575..2165876,2165928..2166734,
2166796..>2167246)
benzoate 4-monooxygenase
85% to CYP53A1, 79% to 53A3 seq looks correct
MITDLLSLQNAGLILLGLIAVYYVIPYLQKWHLHDIPSPRFAAFSNLWLLLQARRGRRFLKVDEAHKKYG
KLVRIAPKHVSIADDAAIQAIYGHGNGFLKADFYDAFVSIRRGLFNTRDRAEHTRKRKTVSHTFSMKSIG
QFEQYIHQNVELFVQQWTKLAKLNGNPRSGYATIDALNWFNYLAFDIIGDLAFGAPFGMLEKGKDIAEMR
KTPDSEPTYVQAVEVLNRRGEVSATLGCLPRLIPYAKYLPDRFFKDGVQAVENLAGIAVARVNERLKPEV
MEKNTRVDLLSRLMEGKDSNGNKLGREELTAEALTQLIAGSDTTSNTTCAILYWCMSTPGVIPKLQKVLD
EAIPDDVDVPTHAMVKDIPYLQWVIWETMRIHSTSAMGLPREIPPGNPPVTISGHTFYPGDVVSVPSYTI
HRSKEIWGPDAEKFVPERWDPARLTARQKAAFIPFSTGPRACVGRNVAEMELLVMTGTIFRLFEFEMQQD
GPMETREGFLRKPLGLIVGMKRRAVHASV
CYP53A12 Neosartorya fischeri
NFIA_090480
96% to CYP53A12 Aspergillus fumigatus = ortholog
see Fungal pages for seq
CYP53A13 Aspergillus oryzae
GenEMBL BAE60506.1
58% TO CYP53A14, 81% to 53A3
MIAELLTPTGAAYVLTAAVIVYYILPYLQLWRLRDIPSPGFAAFSNLWLMLQYRKGNRFVTVDNAHKKYG
KLVRIAPRHVSIADDEAIQAIYGHGNGFLKADFYDAFVSIRRGLFNTRDRAEHTRKRKTVSHTFSMKSIG
QFEQYIHGNAELFVKQWNRIADTQSNPKTGYATIDALNWFNYLAFDIIGDLAFGAPFGMLEKGQDIAEMR
KSPNDKPSYVQAVEVLNRRGEVSATLGACPSLIPWAKYIPDRFFRDGLEAVENLAGIAVARVNERLRPEV
MANNTRVDLLARLMEGKDSNGNKLGREELTAEALTQLIAGSDTTSNTSCAILYWCLRTPGVIEKLHKVLD
ESIPKDVDVPVHAMVKDIPYLQWVIWETMRIHSTSAMGLPREIPAGNPPVTISGHTFYPGDVVSVPTYTI
HRSKEIWGPDAEQFVPERWDPKRLTARQKAAFIPFSTGPRACVGRNVAEMELLVIVGTVFRLFDFEIQQD
GPMETREGFLRKPLGLMVGMKRRSVAV
CYP53A13 Aspergillus flavus
AFL2G_03223
99% to CYP53A13 Aspergillus oryzae,
1 aa diff, N-term extension deleted
see Fungal pages for seq
CYP53A14 Aspergillus oryzae
BAE56637.1
56% to 53A3
MDGDSCPSCCSTCTSLNVFLCPHIPILTLGLLLVYYVTGYLKRWHLRDIPGPFIAGFSRIWLIVQVRQGY
RSLVVHDLHRRYGKIVRLAPNHISIADESAIQAIYGHGNGFLETDFYNAFLNVDWSIFTTRSRAEHTRKR
KIVSHAFSARSLAQVEQHAHNNMEHLVRQWRKMIDSEEGLDDPYAVIDARVWCNYLTFDIIGDLAFGAPF
GMLERENATVSMRKAPENPEVTLDAVEVLNHRGDVSAAFGICPDLIPYAKWLPDPFFRQGAEAIANLAGV
AGAAVDRRLKMDTSMTEKRGDLLALLIDAEDQAGAKLGHRELTGEAVTLIAAGSDTSSSTLCALLYWVSS
TPRVLWKLQNVLDEVIPVDIEVPYLAMVKKITYLQWVIWEALRIHSTFGQGLPREVPPERGPVEICGHTF
YPGDVLSVPGYTMHHSADIWGIDVEDFVPERWDPRRLTQRQKDSFIPFSEGPRACIGRNLAEMELFVGCA
TLFRLFEFRVEGQGPLKVRERWLRKPVSLQVGIRRRYLDARSS
CYP53A15 Cochliobolus lunatus
EU597483
Nada Krasevec
Submitted to nomenclature committee July 10, 2007
65% to CYP53A1
CYP53A16 Mycosphaerella graminicola
Mgr055
64% to CYP53A11 Nectria haematococca
see Fungal pages for seq
CYP53A17 Uncinocarpus reesii
UREG_05168.1
74% to CYP53A1 Aspergillus niger
model has some missing seq (added back)
see Fungal pages for seq
CYP53A18 Coccidioides immitis
CIMG_09108.2
90% to CYP53A17
see Fungal pages for seq
CYP53A19 Fusarium oxysporum
FOXG_04952
90% to CYP53A8
see Fungal pages for seq
CYP53A19 Fusarium verticillioides
FVEG_03183
98% to CYP53A19 Fusarium oxysporum = ortholog
see Fungal pages for seq
CYP53A20 Fusarium oxysporum
FOXG_02776
92% to CYP53A7
see Fungal pages for seq
CYP53A20 Fusarium verticillioides
FVEG_01611
97% to CYP53A20 Fusarium oxysporum = ortholog
see Fungal pages for seq
CYP53A21 Aspergillus clavatus
ACLA_074210
90% to CYP53A12
see Fungal pages for seq
CYP53A22 Aspergillus terreus
ATEG_07877.1
85% to CYP53A12
see Fungal pages for seq
CYP53A23 Mycosphaerella fijiensis
estExt_fgenesh1_pm.C_10348
88% to CYP53A16 Mgr055 Mycosphaerella graminicola
see Fungal pages for seq
53B Subfamily
CYP53B1 Rhodotorula minuta (a fungi)
GenEMBL D63703(2766bp)
Fukuda,H., Nakamura,K., Shibuya,K., Tanase,S., Gotoh,O., Ogawa,T.
and Fujii,T.
Nucleotide sequence of gene for cytochrome P450rm from Rhodotorula
minuta IFO 1102.
Unpublished (1995)
CYP53B2 Sporobolomyces roseus
estExt_fgenesh1_pg.C_30339
57% to CYP53B1 Rhodotorula minuta
see Fungal pages for seq
CYP53B3 Puccinia graminis f. sp. Tritici
PGTG_08085 + PGTG_08086 revised
54% to CYP53B2 from LNRYP
see Fungal pages for seq
CYP53C1 Ustilago maydis
GenEMBL XM_397620.1
hypothetical protein UM00005.1
in the CYP53 clan part B 54% to 53A1 54% to 53B1
CYP53C2 Phanerochaete chrysosporium
AY321584
55% to CYP53C1
my Scaffold_164a
JGI gene models ug.1.19.1 and pc.1.261.1
MAVIEALTQLDLKSWLLLIPALAIVAHILVWLLDPHGIRSYPGP
LLAKFSDAWLGYVAAQGHRSEVVHDLHKQYGTFVRIAPNHLSIADPDALQVVYGHGTG
TLKSNFYDAFVSIQRGLFNTRSRSEHARKRKIVSHIFSQKSVLEFEPHVRLYVKQLIQ
QWDRLYEAGAKGLSGDDGEGGWRGRNGRVWLDCLPWYNYLAFDIIGDLAFGAPFGMLL
AARDAAPVAVDHEQAMASYGKEKSEVQYIPAVQVINDRGTYSASLGVLPPWMRPIVKL
FPWFRRGQKAVKQLAGIAVAAVAQRLTTPTDRVDLLGKLQEGRDDDGNLMGKEELTAE
ALTQLIAGSDTTSNSSCAITYYLAKYPDAQRKLQQELDEALGSDDEPVSTFDQVKRLP
YLQAVIDEALRIHSTSGIGLPRLVPKGGMTVCGRFFPEGTVLSVPTYTIHRDEEVWGK
DPEVFRPERWFEQDKNAVQKTYNPFSFGPRSCIGRNLANMELLIIVSSILRRYDFVLE
DPDKPFDTMEGFLRKPVECVVGIRRRTL
CYP53D1 Fusarium oxysporum
FOXG_14732
48% to CYP53A10
see Fungal pages for seq
54A Subfamily
CYP54A1 Neurospora crassa
GenEMBL X15033
Neurospora crassa sequence contig 1.1450 (supercontig 261)
The translation below is more accurate than the one in Genbank
MALTQLAHSASALLPLVLPSIAAILAFAIFQRYFAPNPLSNLPIVGEEYHGYEKKRQAYLTKAKDLYLEGYTKFKHGLFRIVTPNR
NSVIVVSPRFLDELKKLPDDVVSFDAANDDSMHTKYTLIPTHEPILPHTIKTSLTPSLPRLNPQLSEEVQAFSQEIAPLMSSSPSD
WAPININSKLLRIVAKVSGRVFIGPELCHDERYLEAAVGYTVSVMEAQRAVERMNPWVRPIAAWRLKEVRKLAQMERDATAFLRPV
VEARREKQRKGEEKDNDMLQWLMDSADQGQGKQHGKWGEDTTTTRKLARLQLAISFAAIHTTTLVTTHAVYSLAADPKLQATLREE
IQSVLQEHKGVFNTSALQAMKKTDSFLKETMRFHPLGQTSFNRKVLRTFALSNGQVIPKGSTIEVPNYAVSRDPEAYPNPDVPDPL
RFYNLRNEAREKGEAEQGASGQFVSVNKEFLTFGYGRHACPGRFFAANEIKMILANLVMTYEMGLVEGETERYRDWDIAAGTIPDP
TKDVMFRKL*
CYP54A1 Neurospora discreta
JGI gene model scaffold_13-snap.17.1
93% to CYP54A N. crassa
see Fungal pages for seq
CYP54B1 Magnaporthe grisea
MG01391.4
45% to CYP54A1 AACU01000043 cont2.256
AI068604 revised to fill in gap
see Fungal pages for seq
CYP54C1 Fusarium graminearum
FG11536.1 AACM01000473 FGcontig1.473_scaffold10
see Fungal pages for seq
CYP54C2 Nectria haematococca (Fusarium solani group)
fgenesh1_pg.scaffold_20000135
Necha1/scaffold_20:362069-363960
75% to Fusarium graminearum CYP54C1 probable ortholog
small gap after GYQK? (5 aa), this seq looks right,
maybe the 54C seq is too long here.
Note LTIEQ can be removed from 54C and
the intron boundary is preserved.
This gene model seems correct
see Fungal pages for seq
CYP54C3 Cryphonectria parasitica
CB690595.1 60% to CYP54C2 N-term only, 55% TO 54C1
MATSTTSSLSETLSERFLVIVAAVLACALAYLGPKIISIIRLAALPVIGTE
LGNEEKRRQAYLQGARKLYYAAYDNFKNGLFKGAARLTTSRNANVIVVSPKFLPELNKLP
DSVVSMEAAVDDAMETKYTKIESHVPIIPHTVTGKLTPSLTRLNPTIARETAEALEL
EMPLREFTNWQEVNIHEKLLRIVGMXSGR
CYP54C4 Cryphonectria parasitica
CB686707.1 CB688482.1
55% TO CYP54C1
TRPMPTCEDWTAVKINSVLLRIVAKASGRIFVGPELCHSEEYLEAAIRYTVEVIGAANAV
SNVPPWLRSFKASKLPEVQRLHERRKHAIKFMQPVVESRKDLDQKPDDLLQWLIDNEGNL
GDMSTWKLARTQLALSFAAIHTSTVVSTNVFYTLAVMPNEVILELRDEIRSVLGENNGNFT
SSALQSMKKVDSFIKETMRYYPFANHSFERKVMRTFTLSNGQVIPAGVILECSTAHNQDD
EVFPDASRFDPWRFSKLQEQEKEDGMDGAARHQMVSVTPNHLTFGYGRHACPGRFFAINX
IKMIIGTFLLNYDIKNIDGVK*
CYP54C5 Fusarium oxysporum
FOXG_01238
82% to CYP54C1
see Fungal pages for seq
CYP54C5 Fusarium verticillioides
FVEG_00258 revised
90% to CYP54C5 Fusarium oxysporum = ortholog
see Fungal pages for seq
55A Subfamily
CYP55A1 Fusarium oxysporum
GenEMBL D14517 (2598bp)
Tomura,D., Obika,K., Fukamizu,A. and Shoun,H.
Nitric oxide reductase cytochrome P450 (P450nor) gene,
CYP 55, of the fungus Fusarium oxysporum containing a
putative FNR site in its upstream flanking region.
unpublished (1993)
CYP55A1 Fusarium oxysporum
PIR A40401 (403 amino acids)
Kizawa, H., Tomura, D., Oda, M., Fukamizu, A., Hoshino, T.,
Gotoh, O., Yasui, T., Shoun, H.
Nucleotide sequence of the unique nitrate/nitrite-inducible
cytochrome P-450 cDNA from Fusarium oxysporum.
J. Biol. Chem. 266, 10632-10637 (1991)
Note: This sequence is probably a bacterial sequence related to the
105
family.
CYP55A1 Fusarium oxysporum
GenEMBL D14517 (2598bp)
Park, S.-Y., Shimizu, H., Adachi, S.-i., Nakagawa,A.,
Tanaka, I., Nakahara, K.,
Shoun, H., Obayashi, E., Nakamura, H., Iizuka, T. and Shiro, Y.
Crystal structure of nitric oxide reductase from dentrifying fungus
Fusarium oxysporum.
Nature Structural Biology 4, 827-832 (1997)
CYP55A1v2 Fusarium oxysporum
FOXG_12350
96% to CYP55A1v1
see Fungal pages for seq
CYP55A1 Fusarium verticillioides
FVEG_10773
96% to CYP55A1v2 Fusarium oxysporum
see Fungal pages for seq
CYP55A2 Cylindrocarpon tonkinense (a denitrifying fungus)
GenEMBL D78511
Shoun,H.
nor1
CYP55A3 Cylindrocarpon tonkinense (a denitrifying fungus)
GenEMBL D78512
Shoun,H.
nor2
CYP55A4 Trichosporon cutaneum (a basidiomycete fungus)
GenEMBL AB052733
Zhang,L., Takaya,N., Kitazume,T., Kondo,T. and Shoun,H.
Purification and cDNA cloning of nitric oxide reductase cytochrome
P450nor (CYP55A4) from Trichosporon cutaneum
Eur. J. Biochem. 268 (11), 3198-3204 (2001)
Submitted to nomenclature committee Oct. 11, 2000
P450nor
66% to CYP55A1
CYP55A5v1 Aspergillus oryzae
GenEMBL AB055659
Kaya,M., Hata,Y., Kawato,A., Abe,Y. and Akita,O.
Aspergillus oryzae CYP55A5 gene for cytochrome P450nor
Submitted to nomenclature committee Jan. 23, 2001
60% to 55A1
CYP55A5v1 Aspergillus oryzae
GenEMBL BAC01275.1
cytochrome P450nor
MNSEPVYPRFPFARPSGDEPPAEFHRLLRECPVSRVELWDGSHPWLVVKHKDVCEVLTDPRLSKVRQRDG
FPEMSPGGKAAARNRPTFVDMDAPDHMHQRSMVSAFFNDEYVESRLPFIRDTVQHYLDRLIRAGKDGKEV
DLVKHFALPIPSHIIYDILGIPIEDFEYLSGCDATRTNGSSTAAAAQAANKEILEYLERLVDKKTTNPSH
DVISTLVIQQLKPGHIEKLDVVQIAFLLLVAGNATVVSMIALGVVTLLEHPDQLSRLLEDPSLSNLFVEE
LCRFHTASALATRRVATVDIELRGQKIRAGEGIIASNQAANRDPEVFPDPDTFDMFRKRGPEEALGFGYG
DHRCIAEMLARAELETVFSTLFQTLPSLKLAIPKSEIQWTPPTRDVGIVGLPVTWDRD
CYP55A5v2 Aspergillus oryzae
GenEMBL BAE61721.1
6 aa diffs to CYP55A5v1
MQLEPVYPRFPFARPSGDEPPAEFHRLLRECPVSRVELWDGSHPWLVVKHKDVCEVLTDPRLSKVRQRDG
FPEMSPGGKAAARNRPTFVDMDAPDHMHQRSMVSAFFNDAYVESRLPFIRDTVQYYLDRLIRAGEDGKEV
DLVKHFALPIPSHIIYDILGIPIEDFEYLSGCDATRTNGSSTAAAAQAANKEILEYLERLVDKKTTNPSH
DVISTLVIQQLKPGHIEKLDVVQIAFLLLVAGNATVVSMIALGVVTLLEHPDQLSRLLEDPSLSNLFVEE
LCRFHTASALATRRVATVDIELRGQKIRAGEGIIASNQAANRDPEVFPDPDTFDMFRKRGPEEALGFGYG
DHRCIAEMLARAELETVFSTLFQTLPSLKLAVPKSEIQWTPPTRDVGIVGLPVTWDRD
CYP55A5 Aspergillus flavus
AFL2G_09432
99% to CYP55A5v2 Aspergillus oryzae, 2 aa diffs
Supercontig 11: 146268-147914 (-) strand
see Fungal pages for seq
CYP55A6 Neurospora crassa
AABX01000354.1 cont3.356 NCU06137.1 (version3)
Neurospora crassa sequence contig 1.183 (supercontig 22)
1c8.tfa_90cg@1C8
70% to CYP55A1
EST h8b02ne.f1 in lower case
mgaltenlefpfrrasgldpppeyawlrktdpvtqvrlfdgslawlvtkyhdvCQVATDERLSKERTRPGFPELA
AGGKEAAKNRATFVDMDPPKHMQYRGMVQPIFEMEHVKELEPYIQKTVDDLLERMKNMGCEGGPVDLVQNFALPVPSY (?)
IIYTILGVPVEDLDFLTQQNSIRTNGSSTAREASAANQ (?)
ELLNYLRRLAEQRLQEPKDDLISKLMTEQVKAGKLDTADAVQMAFLLLVAGNATMVNMIAL (0)
GIVTLFLNPSQLEELKRDPSVVPAFVEELSRFHTASAMAIRRVAKEDL
EIRGKTIKKGEGVIASNLSANRDEEVFKDADKFDMHREWPVKELGFGWGEHRCIAEFLAKAELKAVF (?)
ETIFKKLPDLKIAVPFDEIEYTPLDRDVGIVKLPVTW
CYP55A6 Neurospora discreta
JGI gene model estExt_fgenesh3_pm.C_80311
95% to CYP55A6 N. crassa
see Fungal pages for seq
CYP55A7 Trichosporon domesticus SBUG 752 (a basidiomycete fungus)
GenEMBL AY044231 and AY044232
Dirk Patzak
Submitted to nomenclature committee March 4, 2002
124 amino acid N-terminal fragment
77% to 55A4
CYP55A8 Fusarium graminearum
FG11585.1 AACM01000475.1 FGcontig1.475_scaffold11
see Fungal pages for seq
CYP55A9 Nectria haematococca (Fusarium solani group)
fgenesh1_pg.scaffold_30000098
Necha1/scaffold_30:272059-273639
92% to CYP55A2 Cylindrocarpon tonkinense probable ortholog
genome annotation in progress
79% to CYP55A8 Fusarium graminearum
This gene model seems correct DRN 2/5/06
see Fungal pages for seq
CYP55A10 Nectria haematococca (Fusarium solani group)
fgenesh1_pg.scaffold_28000090 [Necha1:94366]
Necha1/scaffold_28:245547-247185
96% to CYP55A3 Cylindrocarpon tonkinense probable ortholog
genome annotation in progress
Note: the very high similarity suggests a very close
relationship between the two species. This is higher
than the ortholog pair matches between
F. graminearum and N. haematococca
This seq is originally of bacterial origin (P450 B type
not E type) similar to CYP105s
This gene model seems correct DRN 2/4/06
see Fungal pages for seq
CYP55A11 Nectria haematococca (Fusarium solani group)
estExt_fgenesh1_pg.C_880004 (better model)
Necha1/scaffold_88:11555-13495
58% to CYP55A3 Cylindrocarpon tonkinense
69% to AB226066.1 Aspergillus oryzae cDNA
58% to fgenesh1_pg.scaffold_28000090
54% to CYP55A8 Fusarium graminearum
same as fgenesh1_pg.scaffold_88000004 which is too long at
SNAQAHPAP between MHQ and RQM
Note: This gene has one extra intron at the N-terminal
that only codes for 3 amino acids
This gene model seems correct DRN 2/5/06
see Fungal pages for seq
CYP55A12P Aspergillus niger
gw1.11.1089.1|Aspni1
48% to CYP55A5v1 aa 53-257, this is a pseudogene
see Fungal pages for seq
CYP55A13 Uncinocarpus reesii
UREG_06342.1
71% to CYP55A5v1 Aspergillus oryzae
see Fungal pages for seq
CYP55A14 Histoplasma capsulatum
HCB04560.1
73% to CYP55A13
see Fungal pages for seq
CYP55A15P Fusarium oxysporum
FOXG_14888
74% to CYP55A2 Cylindrocarpon tonkinense
see Fungal pages for seq
CYP55A16P Fusarium oxysporum
FOXG_17502
83% to CYP55A11
see Fungal pages for seq
CYP55A17 Aspergillus terreus
ATEG_06140.1
73% to CYP55A13 Uncinocarpus reesii
see Fungal pages for seq
CYP55A18 Trichophyton rubrum
ESTs DW700172.1, DW689200.1, DW681558.1
72% to CYP55A14 Histoplasma capsulatum
see Fungal pages for seq
CYP55B1 Chlamydomonas reinhardtii (green algae)
See Chlamydomonas pages
CYP55C1 Aspergillus niger
fgenesh1_pm.C_scaffold_5000072|Aspni1
40% to CYP55A1, 40% to CYP55B1
see Fungal pages for seq
56A Subfamily
CYP56A1 Saccharomyces cerevisiae
GenEMBL X55713(2265bp) PIR S13502 (489 amino acids)
Briza, P., Breitenbach, M., Ellinger, A. and Segall, J.
Isolation of two developmentally regulated genes involved in
spore wall maturation in Saccharomyces cerevisiae.
Genes Dev. 4, 1775-1789 (1990)
CYP56A2 Saccharomyces paradoxus NRRL Y-17217
GenEMBL AABY01000059.1
91% to CYP56A1
46799 MELFKLLGLTLFLTISYIAFAIIVPPLNFPKNIPTIPFYVVFLPVVFPIDQTELYDLYIR 46620
46619 ESMEKYGAVKFFFGSRWNILVSRSEYLAQIFKDEDTFAKSGNQKKIPYSALAAYTGDNVI 46440
46439 SAHGAVWRDYRNAVTNGLQHFDDTPIFKNAKLLCTLIKRRLQEGQLSIQMGPLSQRLALD 46260
46259 NISQVALGFDFGTLKHEKNAFHEHLIRIKKQIFHPFFLTFPFLDLLPIPSRKKAFKDVAS 46080
46079 FRELLVKRVQDELVNTYKFEQTTFAASDLIRAHNNEIIDYRQLTDNIVIILVAGHENPQL 45900
45899 LFSTSLYLLAKYSNVWQERLRQEVNGVTDPKILADLPLLNAFLLEAVRLYPPLSTIINRC 45720
45719 TTRTCKLGPEIVIPKGVYVGYNNFGTSHDPRTWGATADDFKPERWGSDIETIRKNWRMAK 45540
45539 NRCSVTGFHGGRRACLGEKLALTEMRITLAEMLKQFRWGLDPEWEEKLTPAGPLCPLNLK 45360
45359 LKFTENITE* 45330
CYP56A3 Saccharomyces bayanus MCYC 623
GenEMBL AACA01000139.1
83% to CYP56A1
23165 MSILQLIGFILLIIISYIAFVVVVPPLNFPRNIPTIPFYVVFLPVIFPIDQTELYDLYIR 22986
22985 EPMEKYGAVKIFFGSRWNILVSRSEYLAQIFKDEDTFAKSGNQKKIPYSALAAYTGDNVI 22806
22805 SAHGKVWRDYRNAVTNGLQHFDDAPISKNAKTFCNLIKKRAQNGESSIQMGPLSQRLALD 22626
22625 NISQVALGFDFATLTQDKNALHELLVHIKKQIFHPFFLAFPFLDLLPIPSRRKAFRDVAS 22446
22445 FRELLVKRVQDQLINNYKFEQTTFAASDLIRAHNNEVIDYKQLSDNIVIILVAGHENPQL 22266
22265 LFNTSLYLLAKYSTEWQNSLRQEVESVTNSKALADLPLLNAFLFEAVRLYPPLSTIINRC 22086
22085 TTKLCKLGADIVIPKDVYVGYNNFGTSHDPKNWGPTANDFRPERWGSDIETIRKNWRIAK 21906
21905 NRCTVTGFHGGRRACLGEKLALTEMRITLAEMLRQLEWTLDPEWEEKLTPAGPLCPFNLK 21726
21725 LNFKERLPS* 21696
CYP56A4 Saccharomyces castellii NRRL Y-12630
GenEMBL AACF01000075.1
63% to CYP56A1
43945 MPALRVILSLLIVLVGYVIFKVVWPPLNFPRNIPTIPCYVVFLPIIFDIDQVDIYNTYIK 43766
43765 VQMEKYGAVKIFFGSRWNILVSKPEYLAQIFKEEDVFAKSGNQKKIPYSAIAAYTGDNII 43586
43585 SAHGDVWRKYRSIMTNGLQHFKEEPFICNAKKFCGLLRKNRGPTG--NVLMGPLIQRLTL 43412
43411 DNISQVVLGFNFGTLSEETNELHQHLIKIKKQIFHPFFLTFPFFDMLPIPSRKKAFQDIS 43232
43231 KFREVLVSRVKNDLINNYKFEQTSYVSSDLIKAYNNNIIDYKQLTDNIVILLVAGHENPQ 43052
43051 LLLSNCLYLLAKYPS-WQKLIWKETQNISNYKDLNELPLFNSFLFEVVRLYPPLNIIINR 42875
42874 KTNKKCRMGQDIIVPKNTYVGYHNFGVTHNRNVWGNTAEEFDPRRWGTNIADIMIAWRSS 42695
42694 KNSCKLTSFHGGRRACLGEKLGFVEMRISIFEIIQQFELTLPKEWKEKMTPAGPLCPYNL 42515
42514 QLNIKSREKDVGVCE*42467
CYP56A5 Candida glabrata strain CBS138
GenEMBL CR380952.1, GenPept CAG59007.1
57% to CYP56A1
MLNILVFGFICLVLCWILTICFPPISFPNNIPTIPFYSVFIPQY
YNIDQVQFYNLYIREKLEKYGAVKFYFGSRWNILVSRPEFLNNIFKNEDTFAKSGNQQ
KIPYSVLAAYTGDNVISAHGVNWQKYRKPIKDGLQHFDIGILLKNAMKFCELIKEDMR
PFKYHCNFKIIPYIQRLTLDNICRVGLGFEFGAIDEDNNSLHRQLIQIKKQIFDPFYL
NFPMFDRLPIPSRLLAFQNVENFRSSLVDKVQKQLIKTYKFEQANTSGSALVRAYNNG
ELTYKQLTDNIVILLVAGHENPQLFISNLIYLLGKYHDTWQVDIRNEILNNEENNLSE
LPLLNSFLYECLRYYPPLSVIINRKTTKRCMLGPGIVVPKDTYVGYHNYSTCHDSNFW
GHTSDIFDPTRWGKDIETINKAWKTTKNNCILNTFHGGKRACLGEKLVFVSTRIIIAE
FLSSFEWKLSPLWVEQMTHAGPLCPKDLALDIRVRDTSMQIQKLNKYL
CYP56A6 Ashbya gossypii (= Eremothecium gossypii)
GenEMBL AE016819, NP_985947.1
54% to CYP56A1
MTANIVLVLLGGLVLNYIRSIVLPPWGFPRNIPTIPFYVTFLTT
LFDIDQKELFELYIRKPMEKYGAVKIYFGNRWNILVSKPEFLAQMFKDEDTFAKSGNH
IKIPYSILALYTGDNVISSHGLAWKKFRGALTQGLQFFDPSPLSANAEKFIGFIERDM
KKNNGEVLMPALIQRLALANIAQIALGFDIGTLDPERPSKLQQQLEEVKKHIFHPLYM
NFSFLDRLPIPSRIRARQQVERFRSDLLQEVRRNLIINYKYEQTSYAASDLIRVYEHG
EITEKQLTDNIVILMVAGHENPQLLLTTCLYMLAKYPEWQARLRQQAVTLQDDLLNDS
VEFNQFLFETVRVLPPLGQIINRKTSHKCKLGPEIVLPKDTYVGYQVYGTGTSTQVWG
PDAAEFKPERWGSTNATVHETWRRSKNTGAMGAFHGGRRACLGEKMGLMETRVALAHM
LRKLEWTLSPNWKDRVTPAGPLCPFMLKLQFTRIQEPLAAELTA
CYP56A7 Kluyveromyces lactis NRRL Y-1140
GenEMBL XM_452498, KLLA0C06743g
49% to CYP56A1, 53% to 56A6
MLSLVLGIISSGILLSFLCLVCFPIGLPWSLPTIPIYVSFLPIL
TDMDQLEIFERYIRHPMEKYGAVKIFFANRWNILTSKPEFLNIMFRDEDTFAKSGNQK
KIPYSLLAKYTGDNVISAHGKVWMKYRSCIQNQLNVFDSKPLIDNAQLFVKLLKKSIE
DNTEGTVLLPPLIQRLTLANIAKIALGFDIGTLKFDEHDNICRSRLHEKLNQVKQQIF
KPVFLSFPFLDLLPIKSRQRAKIDIIQFRDMLLDTVYKNLIHNYKFEQTNNAAAGLIR
EWKLQGITDEQLKDNLVIILVAGHENPQLLLSTLFYLLAKHQNWQYAIREELLSVDRN
EDILNSFKLTAFIYEALRMFPPLGQIINRRTTRKCQLGPGIIIDKDVYCGYNVYGTGT
ATSVWGETAKQFIPERWGENSNELANNWKKHKYDASMSAFHGGRRSCLGEKLALMEMK
YVLYYVLMNFHIELHPQWKEKMTPAGPICPKMLKVKLTILKDPQDQRSDSLYSSAGET
LSENI
CYP56A8 Saccharomyces mikatae
Smik_4197
90% to CYP56A2 Saccharomyces paradoxus partial seq
see Fungal pages for seq
CYP56A9 Saccharomyces kudriavzevii
AACI02001147.1, AACI02000561.1
85% to CYP56A3
see Fungal pages for seq
CYP56A10 Saccharomyces kluyveri
AACE03000001.1 Sklu_Contig1634.3
Cyan region is an insertion
60% to CYP56A3 Saccharomyces bayanus w/o the insert
see Fungal pages for seq
CYP56A11 Kluyveromyces waltii
kwal_162-g20.1
59% to CYP56A10 Saccharomyces kluyveri
similar insertion as in 56A10
see Fungal pages for seq
CYP56A12 Kluyveromyces polysporus
Kpol_364p9
62% to CYP56A3 Saccharomyces bayanus
see Fungal pages for seq
CYP56B1 Aspergillus nidulans
AN2706.1
43% to CYP56A1 547 clan
revised 7/18/07
MLVTLTVLFLGLVGILASFLTYLFTPPQFFPKGLPTIPFYYTLIPLLNQT
LKRAPTDQVTLYHRYLSTPLRTHGAVKLFFGGRWNILITKPSYIAEVLKN
EDLYAKSGNQKKIPHSVLAQYTGDNIISSHGENWKLYSSIFKPGLQRDYD
PSGIWRNASLLVQMISQDIKRKSPVDINPLMQRYALANLSEVLLGTTFDT
LQKPAASLHAFQLLIKPKIFDPIFLNFPVLDYLPLQTREEARKLVTRFTD
ELIETVRKGHTTCDHEKEHTRNLGCRLLYACESGLFTEMQLRHNMISAFL
AGHENPQLLLVSSLFLLAEHPEMQESLRAEISALNDLEPAYNALSALPLL
TSTIYEVLRLYPPISQLINRRTTAPTLLGGEIPIPAGTYVGYNAYATNRD
IGFWGPDANEFKPSRWGNTMEEINALFRRANAKGAFISFHGGRRTCLGQR
FALLEGRVTLAKLLMCVRWEIDPSWQRGMTPAGPLYARNLQLRFSNISGAGKAGAS
CYP56B2 Uncinocarpus reesii
UREG_00550.1
59% to CYP56B1 Aspergillus nidulans
see Fungal pages for seq
CYP56B3 Coccidioides immitis
CIMG_00523.2
80% to CYP56B2 Uncinocarpus reesii
see Fungal pages for seq
CYP56C1 Aspergillus oryzae
GenEMBL AP007164.1c
third P450 of six on this accession
CDS join(1160825..1161472,1161531..1162415,1162505..1162558)
47% to CYP56B1 Aspergillus nidulans
41% to CYP56A1 S. cerevisiae
MVMHGLCIIIGKALAVGILFLSICIFSRIVPPRYPRNIPAVPFW
VTLLSLVRDIDQEDIYRRHIQKPLQTHGAIKIFFAGQWNLLIQRSSYLNEIFRNEDVY
QKSGNQKKIPHSVLAEFL (1?)
GDNVISSRGTTWRLYRDIITPG
LQGHFDAGLIAANAEELCSSLLAFQNTVGNCGVPVQDLLQQFTIANVSQVLLQANHKP
TKGDESSLHQLQLAVKREIFKPIFMNFPVLDRLGKLIPCRVRARNVVEQFSAALQYGV
RHGQGTPNASNLGARLMAARDGGVLTGKQFRDNLNVLFVAGQENPQLLLISMLYLLAKHP (0)
DVQSRLRREIDACSTLDPSNVAFSELPYVTCVIYESLRLLP
PISQLINRRTSQDVILGNQIYIPKGTYVGYNCYSTNRDPAVWGPTADEFRPERWGQSN
TEISQCYRQRRARAEFVSFHGGSRACLGEKFALLEARVALFVLVSRLSWSLDPEWPDR
KTPAGPLYPRALRLIFTERK
CYP56C1 Aspergillus flavus
AFL2G_06061
98% to CYP56C1 Aspergillus oryzae
see Fungal pages for seq
CYP56C2 Uncinocarpus reesii
UREG_04911.1
60% to CYP56C1 Aspergillus oryzae
see Fungal pages for seq
CYP56C3 Aspergillus clavatus
ACLA_064250
53% to CYP56C2
see Fungal pages for seq
CYP56D1 Clavispora lusitaniae ATCC 42720
GenEMBL AAFT01000066.1, CLUG_02306.1
46% to CYP56A1
MLIDIIISNISVSFWCLCFFFLSWKIFRIIYPPFDFPKNIPTIPFYAAFVSSFLGWDQNK
VYAHYYRENLEKYGAAKIYFASRWNILVTKPEYLAQIFKQNEIFEKSGNQHKIPYSLLAE
YTGDNIISTGNKYWKLYRKAVTNSIQFPNLEPLQTNATVLIENLSAASDNGYFPIADILQ
AYTLNNVGDCVIGTNFHKSDNFDISTSLKKVKQEIFRPLFLNFPLLDLLPFPSRIRARKL
VRSFKRNYCNKILNELNEENKGRLGHNLATFWKQGDITEKQFQDNAMIAMIAGHENPQLL
LTSLIYVIAKYPEIQRQLRSEMNSSNYDVKEQIPLLKAVVYETIRLFPPLGLIVNRKTRK
NVILGNGIFIPKGTYVGYNNFGTQRDRNYWGDDADAFRPERWGNDGKKIEEKYAEAKSKC
TLPAFHGRSRACLGEKFALAEVRKAVIEILQNFEISLDPSWKEQLTPAGPICPLKLSVML
KKVCSEHEHNI*
CYP56E1 Candida tropicalis MYA-3404
GenEMBL AAFN01000070.1
45% to CYP56A1
MIMFDIVKYTLIGLFSYLLYVILDIVLPPFNFPKNIPTIPFYVSFLGAYTNLDQRDI
YNLYLREKLEKYGAVKIYFASRWNILITRPEYLLEMFRNEDVYSKRGNHLKIPGSVMATY
TGDNIISAHGELWKLYREVIAKSIQFPDFEPITKNTKSLLEIIDGMIDSDKNHAIIPITD
LFQKYSLANVTESILGVNFKVLEGDQSIMHQKIKYVKLQIFKPFFLNFPYFDSFPIPSRL
QARKEVINFRNWYGQSIIDKHDPQLPNSAATKLVDGLMQEKLTEKQFLDNAIIVMIAGHE
NPLLLMLSLMFVAAKYPKVQEAIRSEIDPTKPYLHSVIYETLRMYPPLGLIINRYTTRPT
KLGNIVIPKGVYCGYNNFGTGRDRNVWGPDSDEFKPERWGRDNIEEINRNYANAKRSAEL
PAFHGRKRACLGEKYALYEVKELLTSILGHYKVTLDASWKEKITPAGPISPFGLKVKFEK
LIVA*
CYP56E2 Candida albicans SC5314
GenEMBL AACQ01000054.1
45% to CYP56A1 missing N-term runs off the end of the contig
whole seq on orf19_554
151305 DFLLEMFKNEDVFAKSGNHVKIPNSVLATYTGDNIISAHGELWKLYRDVVAQSIQFPDLK 151126
151125 PISKNTQKLLRFLDNEMGSANQATISVTDILQKYSLANVCESVLGVNFNVLDDKQSLMHE 150946
150945 KIKYVKLQIFNPIFLNFPYFDNFPIPSRLKARREVIGFRKWYGQSLIEKYNLQLPN 150778
150777 SAATKLVDSLIKEKLTEKQFLDNAIILMIAGHENPLLLMLSLLYVVSKYPQVQELIRNE 150601
150600 TETTKPYLHSVIYETLRMYPPLGLIINRCTTRITKLGNIVIPKNVYCGYNN 150448
150447 FGTGRDRNVWGSDADIFKPERWGLEIDEINKKFTLAKRSAELPAFHGRKRACLGEKYALF 150268
150267 EVKQFLLAILGEYKVSLDPNWKEQLTPAGPISPLRLKLNFEKLTVS* 150127
CYP56E3 Candida dublinensis
cdub_14-g334.1
96% CYP56E2 Candida albicans (ortholog)
see Fungal pages for seq
CYP56E4 Candida parapsilosis
CPAG_05515
65% to CYP56E1 Candida tropicalis
see Fungal pages for seq
CYP56E5 Lodderomyces elongisporus
LELG_03266
runs of EE probably in an intron seq
59% to CYP56E2 Candida albicans
see Fungal pages for seq
CYP56E6 Pichia stipitis
JGI model e_gwh1.2.1.1913.1
57% to CYP56E4 Candida parapsilosis
see Fungal pages for seq
57A Subfamily
CYP57A1v1 Nectria haematococca MP VI (Fusarium solani group)
GenEMBL L20976 (3276bp) S70757 (1373bp) PIR S45583 (515 amino
acids)
Maloney,A.P. and VanEtten,H.D.
A gene from the fungal plant pathogen Nectria haematococca that
encodes the phytoalexin-detoxifying enzyme pisatin demethylase
defines a new cytochrome P450 family.
Mol. Gen. Genet. 243, 506-514 (1994)
PDAT9 pisatin demethylase
CYP57A1v2 Nectria haematococca (Fusarium solani group)
no accession number
Kevin McCkluskey and Hans Vanetten, Univ. Arizona
personal communication
pda4 pisatin demethylase
Note 98% identical to CYP57A1v1, probable allele
CYP57A2 Nectria haematococca (Fusarium solani group)
GenEMBL X73145 (2750)
Reimmann,C. and VanEtten,H.D.
Cloning and characterization of PDA6-1 a fungal gene encoding a
cytochrome P-450 which can detoxify the phytoalexin pisatin from
garden
pea.
Gene 146, 221-226 (1994)
high similarity to CYP57A1
CYP57A3 Nectria haematococca (Fusarium solani group)
fgenesh1_pg.scaffold_15000311
Necha1/scaffold_15:835216-837380
61% to CYP57A1
see Fungal pages for seq
CYP57A4 Nectria haematococca (Fusarium solani group)
fgenesh1_pg.scaffold_30000136
Necha1/scaffold_30:357529-359333
63% to fgenesh1_pg.scaffold_15000311, 57% to 57A1
This gene model seems correct DRN 2/5/06
see Fungal pages for seq
CYP57B1 Nectria haematococca (Fusarium solani group)
gw1.57.27.1
Necha1/scaffold_57:81298-82977
43% to CYP57A3
in CYP57 family, model short at N-TERM
see Fungal pages for seq
CYP57B2 Nectria haematococca (Fusarium solani group)
83% to CYP57B1
see Fungal pages for seq
CYP57B3 Aspergillus oryzae
GenEMBL BAE55311.1
56% to 57B2, 55% to 57B1
MIGTVLDTVLGNPQGAVGGLFVGSFVIFWVVPFLYNLFFSPLRNVPGPFWARFTILWEFSQLMKGRSHEE
YIKLHKKYGPVVRVSPKRYSVIDPQDVKKIYGFGADFPKSEFYDSLGDPKNIFTVRDNEDHKDRRRKVAS
LYTMSSMVAYEDAVDRMTTLCIKKMTDLAASRKLISIPKFMQFYAFDVIGEITFDQNFGMMENMGDTQGI
IKEIHAMNNTIGIMGLLPELQSTWRAAQKVLFSGTAMGKLTEYIWSQYWKHRNTNSGAKQKSQYDTFLRK
VLELEAAHKVGRMNVLDSCGSNIGAGSDTTGITLSAALWYIYRNPDKLAKLRHEIDTMTAEGRISDPVTY
KQAQEMPYLNAVIKETLRVHPGVGTILARVVPKGGITLTGGYYVPEGTHIGTNAWPLHYSETVYGPDADQ
YRPERWLEDKPQPDYRDSMMFAFGSGSRTCIGRNISLLEITKVLPQIVRKFDLKFEEKKDPWDAWCAWFV
YPKYKCWIEPRKPVENVA
CYP57B3 Aspergillus flavus
AFL2G_00227 revised
100% to CYP57B3 Aspergillus oryzae
see Fungal pages for seq
CYP57B4 Fusarium oxysporum
FOXG_01386
81% to CYP57B2
see Fungal pages for seq
CYP57C1 Mycosphaerella fijiensis
fgenesh1_pg.C_scaffold_2000556 revised N-term
51% to CYP57B3 Aspergillus oryzae
see Fungal pages for seq
58A Subfamily
CYP58A1 Fusarium sporotrichioides (filamentous fungus)
GeEMBL U22462 PIR S57337 (520 amino acids)
Hohn.T.M., Desjardins,A.E. and McCormick,S.P.
The Tri4 gene of Fusarium sporotrichioides encodes a cytochrome P450
monooxygenase involved in tricothecene biosynthesis.
Mol. Gen. Genet. 248, 95-102 (1995)
Note: also called TOX4
CYP58A2 Myrothecium roridum, complete cds.
GenEMBL AF009417 (4058bp)
Trapp,S.C., Hohn,T.M., McCormick,S. and Jarvis,B.B.
Characterization of the gene cluster for biosynthesis of
macrocyclic trichothecenes in Myrothecium roridum
Mol. Gen. Genet. 257 (4), 421-432 (1998)
(TRI4) gene
CYP58A3 Fusarium graminearum
CYP58B1 Aspergillus parasiticus
No accession number
Ken Ehrlich
Submitted to nomenclature committee 12/30/2003
40% to 58A1, 37% to 58A2 on the border of the CYP58 family
gene name CypA in aflatoxin biosynthesis
CYP58B1 Aspergillus parasiticus aflatoxin pathway gene cluster
GenEMBL AY371490
join(3110..3314,3377..4218,4278..4388,4464..4630,4700..4871)
gene="aflU"
MASNTVYTSLIGLLVALTVRSIYRVYFHPLRKIPGPKIAAITHL
YQHYYDAVKGGKYIWKLDELHRKYGPIVRFNPNEVHIQDSHYYHHIYAGGAKKQDKDP
GFPAVPLFPGVTVTTIKHNHHRLRRGIIKSFFSKQYVTGLEHVIQSKVNLLASRFTEA
YRHGTVLDLKYVFAALTSDLTTHYVYGTNLNHLAEPDFKNDFLAGMDSVGPWIPVLLV
FGRLLKLARYLPACLVPAGEFLHLWTLSERRVGEILDSQDNGTMGDQKTLLQAMATAD
VSEEEKTATRLQMETLNIIAGGTETTARALAVGVFHLAHKPSLLLQLRDELRTVMPFP
DSSASWTQLEQLPYLAGVVNESLRLSFGFIIRSARVYPNDPLVYEDLVIPPGTPISQS
AYFVCMDPSIFPQPEDFNPDRWVQAAREGNNLHRYLIVFSKGSRHCLGINFALAEIYL
AIATIARRFDLVPYQTTVEQLQMKRDLGFAAPEKGPFTVRAKVTGLAD
CYP58B2P Aspergillus flavus NRRL3357 pseudogene
GenEMBL AAIH01000612.1, AFL2G_07229
missing first 29 aa, two frameshifts and one stop codon,
bad boundary at 1273
76 LRKIAGPKIAPITHLY*HYYDAVKGGKYIWKLDELHRKY (1) 192
255 GPIVRFNPNEVHIQDSHYYHHIYAGGAKKQDKDPEFPAVPLFPGVTVTTIKHNHHRLRRG 434
435 IIKSFFSKKYVTGLEYVIQSEVNLLASRFTEAYHHGTVLDLKYVFAALTSDLTTHYVYGTNLKX 623
626 LAEPDFKNDFLAGMDSVDPWIPVLLVFGRLLKLAGYLPACLVSAGEFLHLWT 781
783 LSERRVGEILDPQDNGTMGDQKTLLQAMATADVPEEEKTATRLQMETLNIIAGGTETTARALAV 974
975 GVFHLAHKPSLLLQLRDELRTVMPFPDSLASCTQLEQLPYSYL (0) 1103
1163 AGVVNESLRLAFGFIIRPARVYPNDPLVNEDLVIPFP (?) 1273
1362 TPISQSAYFVCMDPSIFSQPDDFNPDRWVQAARDGNKLHRYLIVLSKGSRHCLGIN (2) 1529
1599 FALAEIYLAIATVARRFDLVPYQTTVEQLQMKRDLGFTAPEKGPFTVRAKVTGLAD* 1769
CYP58B2P Aspergillus oryzae
Supercontig 10: 19609-20001 +
95% to CYP58B2P Aspergillus flavus
even pseudogenes are well conserved
see Fungal pages for seq
CYP58B3 Aspergillus nomius isolate AN13137
AY510454.1
8295-10054
92% to CYP58B1 Aspergillus parasiticus
gene CypA of aflatoxin biosynthesis
gene model revised
MVSKTVSTALIGLLVALILRSVYRVFFHPLRKIPGPKIAAITHL
YQHYYDAVQGGKYIWKLDELHRKYGPVIRFNPNEVHIQDSHYYHQIYAGGAKKQDKDP
GFPAVPLFPEVTVTTIKHHHHRLRRGIIKSFFSKQYVMGLEHVIQSKVNLLASRLTEA
YRHDTVLDLKYVFAALTSDLTTHYVYGTNLSHLTEPDFKNDFLAGMDSVGRWIPVLLV
FGRLLKLARYLPACLVPAGEFLHLWTLSERRVGEILGSKNNGTMGDRKTLLQAMATAD
VPEQEKTATRLQMETLNIIAGGTETTARALAVGVFHLANKPSLLLQLRDELRTVMPFP
DSTASWTQLEQLPFL (0)
AGVVNESLRLSFGFIIRSARVYPNDPLVYEDLVIPPG (0)
TPISQSAYFVCMDPSIFPHPEDFNPDRWVQA
ACEGHNLHRYMIVFSKGSRHCLGINFALAEIYLAIATVARRFDLVPYQTTVEQLQMKR
DLGFAAPEKGPFTVQAKVTGLAD*
CYP58C1 Aspergillus nidulans
see Fungal pages for seq
CYP58D1 Aspergillus nidulans
AACD01000172.1 Aspergillus nidulans FGSC A4 revised
see Fungal pages for seq
CYP58D2 Aspergillus oryzae RIB40
GenEMBL BAE58658.1
AP007157.1b (second P450 of 8 on this accession)
73% to CYP58D1
complement(join(191351..191525,191581..191747,
191803..191910,191973..192829,192878..193118))
MGVISVVTFLASAKEHFIISILLLFPIALILRTIYRLYIDPLHH
IPGPKLAAISHLYEFYHDVVRGGLFIWEIEKMHQEY
GPIVRINPREVHIKDPYFYDELYAPAHGWRDKDAKSVEIFSSPNA
LVSTVDHHTHRMRRKLLTSFFSRRSIERIEPVIHE
SLSKFLDSLITAYEEDSVVELIDRLQALTGHVITQYAYGEDYGLHEPQNIGKGIVKVV
QEGTEQIHLHRFFPLIQRFLRLIPSFFMTQLFPARAAMYDLLHGVRKKSIEVLQQKDV
CTPTERTTMFHALTAPEVPPEERTLQRLEDEGLVLFAAGTETTATTLGVAIFHILSDP
MVLTKLRKELEQVMPTPEGLATWRELEKLPYLNGVIHEALRFSGLAMRQQRVSPTEVI
KYKDYAIPPGTPVSMLQYFLHTDPALFSDPEKFYPERWMLAAERKESLSRFLVTFGKG
TRSCIGMNLAYAELHTALAAIVRRFDLELYETTAEDIRFVRDKLLPRAKNGPWRVRVK
VVGIRKD
CYP58D2 Aspergillus flavus
AFL2G_03972
99% to CYP58D2 Aspergillus oryzae
see Fungal pages for seq
CYP58D3 Aspergillus fumigatus Af293
GenEMBL XP_753152.1 also AAHF01000003.1
trichodiene oxygenase predicted
71% to 58D1
N-term part of broken gene
MRRKLLNNFFSRRSIENIEPVIHNSLSRFLDGLTTAYKEGSVVELIDRLQGLTGDVITEYAYGENYGLQD
PENYGKGIVKVVQEGTDQIHFFRFFPLVSQLLRIIPSWFMGQVFPARAAIYQLLDGVRKQSILALQQKNV
STAPQRMTIFQALTAPEVPPEERNLQRLQDEGLVLFAAGTETTATTLAVALFYN
CYP58D3 Aspergillus fumigatus Af293
GenEMBL XP_753171.1 also AAHF01000003.1
73% to 58D1
C-term part of broken gene
YILNDKRVLSKLRRELEQI
MPTPQHPTTWRELEKLPYLTAVIREALRFSGIVMRQQRISPTEALKYKDIVVPPGTPVSMIARFVHMDPT
IFPDPTRFHPERWMLAAGREEGLSKFLVTFSRGNRGCIGMNLAYAELYNTLAAVVRRFDLELNQTTPENI
RFVRDKLLPRSKDGSWTVRVKVVGIREQ
CYP58E1 Nectria haematococca (Fusarium solani group)
fgenesh1_pg.scaffold_14000019
Necha1/scaffold_14:58063-59829
48% to CYP58A3, 46% to CYP58A2
46% to 58A2 also 48% to 579A2, check 579A2 vs 58A2 = 65%
Note 58A2 and 579A2 are same family and same subfamily,
already revised names 579A2 = 58A3, not yet changed on blast server
This gene model seems correct DRN 2/5/06
Note this gene is near scaffold_14000017
see Fungal pages for seq
CYP58E2 Aspergillus oryzae
GenEMBL BAE59845.1
61% TO 58E1, 47% TO 58A2, 41% to 58C1, 46% TO 58A3
MLDTISESRPNGLATLGVVVATFATYLALKALYNLYLHPLRKFPGPKLAAIGPYYEFYYDVMKDGMFLWE
MERMHQVYGPIVRVNANEIHIRDPHYYSTVYAGNHRSTDKYHDAVAAFSVPQASLATIHHKVHRLRRSIL
NPYFSKAAVTRLESAINERIERMCSRLEETMHYGQVVDLDAGFAALTADIVTTYFYGQNFDYLGNEGFKF
QVRDAILGLIQFYHFTRFFPWIANTIKKLPIPIMRLIHPGAAYLVSSQEEIKDSIRASLDKGNKADAKSV
IVQALEDPTIPPQERTLDRLGDEGTTIIFAGTETTARALSVGMFHILNNKTILKKLREELDTLPGVSSGV
YSHVQLECLPYLTGAVQESLRLSHGPAIRLPRVANDKALKYGDYIIPPGTPVSLCTVLVHQDPCIFPDPH
RFDPERWVKASKEGVNLDKFIAAFTKGTRQCLGINLAYAEIYLTFAKLIRTFNMEIYDTTVDDLTVHHIR
LTGAPKQGTGEVKVKVTEKIL
CYP58E2 Aspergillus flavus
AFL2G_06877 revised
100% to CYP58E2 Aspergillus oryzae
see Fungal pages for seq
CYP58E3 Neosartorya fischeri
NFIA_042080
53% to CYP58E1
see Fungal pages for seq
CYP58E3 Aspergillus flavus NRRL3357
AAIH02002155.1
Identical to CYP58E3 Neosartorya fischeri
(partial seq, upstream part in a seq gap)
see Fungal pages for seq
CYP58E3 Aspergillus terreus NIH2624
AAJN01000124.1, ATEG_04417.1
93% to CYP58E3 = ortholog
see Fungal pages for seq
CYP58F1 Aspergillus oryzae
GenEMBL BAE64028.1
46% TO 58E2, 40% to 58C1, 45% to 58D1, 44% to 58B1
46% to 58A3
MALLVILGIGFVTYLVFLSIYRLYFHPLSHIPGPKLAAITHGYEFYHNIIRGGLFIWELERLHEVYGPII
RINPREVHIKDPDYYDDIYASSARKREKDPVLVAQFGLEGSGFSAIDQETHHQRRAPVAKFFSKRAIENQ
EHLIRDSLNKLVGHFQEACRAHTVVSLDAAFAALTSDVIHQYAYGFNPNNLDKEGFNAKVRDGINGLFQL
AHLLYFFPILQTLMNVMPLPLLQVLNPPAFALASQKKELYEQGAAALEKANSKAGSGTLIDTLAAPSMPE
HMRSAHRLMNEGFALVIGGTETTARSLALGAWHLFTREDIRNKLREELKQVMPTPDSQPTWNDLEKLPYM
SGVISESLRLSTGIANRSSRVAPTEALVYKDYTIPPGTPVSETNYFTLMDPEIFSDPHAFDPERWIRAAA
KGQRLDRYLVNFSKGSRMCVGLNLAYAELFLVIATIVRRFDIELYETPKSNIDFARDFGTPYPDKGNFSV
RALVTGVISE
CYP58F1 Aspergillus flavus
AFL2G_09007
99% to CYP58F1 Aspergillus oryzae
see Fungal pages for seq
CYP58F2 Aspergillus niger
fgenesh1_pm.C_scaffold_7000005|Aspni1
60% to CYP58F1
see Fungal pages for seq
CYP58F3 Aspergillus terreus
ATEG_04068.1
68% to CYP58F1
see Fungal pages for seq
CYP58G1 Aspergillus oryzae
GenEMBL BAE64022.1
40% to 58C1, 41% to 58D1, 39% to 58B1, 35% to 58A3
39% to 52E2, 41% to 58F1
revised 3/18/2009
MNLPLIWIGLFTAVSYLVIRSIYRLYFHPLSNFPGPKLAAVTHLYEFYYDVVKGGKFIWEMQRMHDQY
GPIVRINPREIHIKDPYYFDPIYTSKGQAKDPYIVRTFATPLSTAATVEHDRHRYRRDLVNPFFSKRSVMGVDYIV
QDKVDKVCKRLTQVHERGTVVSLDDLFAALTADVISHYAYGESLGFLDTENLKNEFRDAVASAGLLCHFA
RFFFVVSMVAETMPALVEWMQPSSKGLWEAKRMIEQMARSSLEKDHEKNANSRKTIFDALCAESVRPEER
TVARVRDEAMVVFGAGTETTARVLATGSYYLYRDKPRLEKLRAEIETVMPDSTDHVSLTQLESLPYLTAV
INESLRMAHSVTMRLPRISPTPLAYKDYIIPPG
TPVSQSVYFMHMDPTLFPNPDSFNPERWLEASSKGERL
TKFLVPFSKGSRICLGMNLAYSELYQMFATLVRCFDLEIQTPPESVRITRDFIIGLPDDADYLKVHSLVT
NAL
CYP58G1 Aspergillus flavus
AFL2G_09004
99% to CYP58G1 Aspergillus oryzae
see Fungal pages for seq
CYP58H1 Aspergillus oryzae
GenEMBL BAE56719.1
43% to 58B1, 44% to 58C1, 42% to 58D1, 38% to 58A3
42% to 58G1, 40% to 58E2, 42% to 58F1
MDEFTISAGSLCLLSLVVGFIIQSVYRLFFHPLRKFPGPKLGAISHLVQGLCFANNPKGPVVRINPRELH
INDPYYYEQIYAGSSRIREKDPRFIGVFTTPLPMVATVGHEHHRIRRGLLSSYFSRRALKKAELIIDQKV
DRLLVRFHSAFKCHAVLPLQRVFAALAADIVSEYCYGASQGYLEQKVFQNQMIDAVNYVMSMCHINKSIP
IIPKLLRCVPVGLMEKLGLQMADVIGVRNLIRRQAAKSLDKEWLSHDTNMLSKNVFDAIAAADVAPQEKT
LRRLEEEGAALFGAGIETTARALTVAMFHLISDETMIRKLRDELKQVMLSPASRPTWAELEQLPYLTGVV
NESLRLSFGLVARSPRVSPIESLAYGEYVIPPG (0)
TPVSQSAYFVHMNPQV
FPEPESFNPERWIKAAEKGQYLSRFLVAFSKGSRQCLGMNLAYAELYLTLARIVRLVDMKLVGTTIDNIR
VGRDLGHPAPKAGNFKVKVEVMGIASKS
CYP58H1 Aspergillus flavus
AFL2G_07403
98% to CYP58H1 Aspergillus oryzae
see Fungal pages for seq
CYP58J1 Aspergillus oryzae
GenEMBL BAE62179.1
41% to 58C1 46% to 58C1, 44% to 58D1, 41% to 58B1,
40% to 58A3, 43% to 58E2, 42% to 58F1, 41% to 58G1,
41% to 58H1
MDSPRLWTGLAGLITYLIVISVYRLFSHPLRNIPGPKLAAVTHLYEWYYDLFLGGKYLFEIERMHERYGP
IVRINPPEIHINDPKYYDEIYASGTHRRNKDAEFVSFTGLLLSSASTTDHDLHRYRRGLMNNFFSKKSVR
GISYFVEEKVHNLMQRFEAFYRCNKVVRLDDAFAAMTSDVITHYCYGKSWDYLDYANLRTDVRKAVRDLT
CSVHFNRIFPIFLAVLKKLPLRWLYAIHPGRSVVLDIQKTIYEQSAEAIHGDKYKIGHNDAVDKHKTIYD
QLTDPSIPAEERSLQRLQDEGLLLISVGTETTARALTTACFHIASDDQLRTRLREELRTVLPTPTSSVTW
SELEKLPYL (0)
TGTVNESLRLGGFLTTRSPRIAPDEPLTYKEYTIPPS (0)
TPVSSSSYFGHKNPNIFPEPEKFSPERWISAGRNNDHLFKYITSFSRGSRICAGMNLAF
LELYMTLAYFVRRFDVELVDTTVEDMKIVRDMRVGFTHRGEPTVYGRIVRVYED
CYP58J1 Aspergillus flavus
AFL2G_06095
98% to CYP58J1 Aspergillus oryzae
see Fungal pages for seq
CYP58K1 Aspergillus oryzae
GenEMBL BAE63021.1
41% to 58J1, 38% to 58A1, 40% to 58D2, 39% to 58C1,
38% to 58B1, 42% to 58J1, 37% to 58H1, 37% to 58G1,
39% to 58F1, 39% to 58E2
MVMDQAWTWMALVAIAGLFWITRIFYRLYFHPLAKIPGPKLAAASHLLEFYYDVILGGKFLFQVEKMHQK
YGPIVRINPKEVHIIDPTFYNEIYASGMRKRDKYEGFVRSLAADESTVSTVGSEKHRFRRSILQNFFSKR
SVMEFSSAIGERVEKLMRRLEVFEKTQTPVALDVVFSALTSDMITYICYGKDWKFLDHKDFNCDIHQAGV
DFANFFHFNRFFPWVFMTLRALSPRMLALLIPGRAATFKFQESLLKHAIEMAANEQSDAPSKETEKSRPN
VISNLINPSIPYMERSRRRLEDEVITILVAGTEAPVKVLSMAMYYLGSEPAIGEKLRAELKTILPARTST
ATYAELEKLPYLHGVVYESLRISDSVIARFPRIAPTETLRYKDHILPPGTPMSCSSYFISRNHDLFPNPE
KFDPERWIHAAEKGENLKQHLTAFTKGSRICLGINLTIAELFLTIAHMCRRYHILLHNTEPEDVCTTSDL
LAGYTRRGVLKVHAKLKAVRE
CYP58L1 Aspergillus niger
e_gw1.12.119.1|Aspni1
49% to CYP58D1, 49% to e_gw1.8.187.1
see Fungal pages for seq
CYP58M1 Aspergillus niger
e_gw1.8.187.1|Aspni1
51% to CYP58K1
see Fungal pages for seq
CYP58M2 Neosartorya fischeri
NFIA_057570
55% to CYP58M1
Note: this seq has no ortholog in A. fumigatus
see Fungal pages for seq
CYP58M3 Aspergillus terreus
ATEG_02828.1
55% to CYP58M1
see Fungal pages for seq
CYP58M4 Aspergillus terreus
ATEG_03531.1
57% to CYP58M1
see Fungal pages for seq
CYP58N1 Aspergillus niger
e_gw1.9.613.1|Aspni1
42% to CYP58C1, 39% to e_gw1.8.187.1
see Fungal pages for seq
CYP58P1 Aspergillus clavatus
ACLA_013370
49% to CYP58H1
see Fungal pages for seq
CYP58Q1 Aspergillus terreus
ATEG_02820.1
49% to CYP58M1, 48% to CYP58P1
see Fungal pages for seq
CYP58-un1 Aspergillus niger
gw1.19.191.1|Aspni1
43% to CYP58J1 pseudogene fragment C-term, 52% to CYP58M1
TPMGSSSDFIRRDPDNFPSLNNFTSTRWLMENGITIHSTRYLVSFSKGSRGRIEM ()
NLPYFELYLTVAYLVR
59A Subfamily
CYP59A1 Emericella nidulans
GenEMBL L27825 (4310bp)
Keller,N.P., Kantz,N.J. and Adams,T.H.
Aspergillus nidulans verA is reqired for production of the mycotoxin
sterigmatocystin
Appl. Environ. Microbiol. 60, 1444-1450 (1994)
gene stcS
note: C-terminal missing
Aspergillus nidulans (same as Emericella nidulans, Emericella is the
teleomorph sexual phase of the fungal life cycle.
GenEMBL U34740 (505 amino acids)
Brown, D.W., Keller, N.P. and Adams, T.H.
complete sequence of CYP59
Proc. Natl. Acad. Sci. USA 93, 1418-1422 (1996)
CYP59A1 Aspergillus nidulans
AACD01000132.1
stcS L27825 revised 7/20/07
MPSFSLLTGHFGALKQTIDGMPPNATLHSIMLKLSQKFRSGMFY
INMWPFSGTWLVVATPSGAAQIQSLNLSKPNILRRPLETITGGPSLMSMHGETWKRWR
ALFNPGFNPNYLIGLAPLIADEVVVFCEQLRQKARTGTVFQLEPLTLRLTVDTICSVT
LYVVTPVGRWPFLTPDLEIHSSTTKLRTTPLPQRCNGRSNGPRLELPSTPLRRYLTVR
PLVMWYNNRLMNRFIDQEVDRAYREQSGRQSKSVISLALRDYMKEKDGSLEDFKRRVA
PQLRVFLFAGRDTTSSTLLYAFYLLSRHPEALAKVRLEHDQVFGPYHQQVHEKIHQDA
KLLNQLPYTTAVLKETLRLFPPSASMREGGPGVEITDDNGQVYPTAGCNVWTLTVALH
HNSAHWAEAESFIPERWLVGSDHPLYPAKGAWRAFEFGPRSCIGQTLAMLELRVALAM
TLREFDIAPAYDKWDHIYPNDAVKEFNGHRAYQAEKGGGGAHPADGMPCLVTFRV
CYP59A3 Aspergillus parasiticus aflatoxin pathway gene cluster
GenEMBL AY371490
join(43951..44429,44479..45478)
gene="aflN"
function="conversion of versicolorin A to
demethylsterigmatocystin
70% to CYP59A1
CYP59A4 Aspergillus oryzae
GenEMBL BAE59520.1 also BAE71324.1
72% to 59A1
MPEFQLLAGHFGTLKKTIQGMPSDATLHSIMLKISQQFPSGIFYINMWPFSGTWMIVSTPSAASQIQKLN
LSKPAILRRPLEMVTGGPSMMSMHGETWKKWRALFNPGFNPAYIIGLAPNISDEVAIFCAQLRKIAQQGE
VFPLESLTTRLTVDSICSVVLDTQLHHQIKDHPLATALQRQIDWTSFGTTFNPFKRYLTIRPLVLWYNNK
FMDRIIDGEVDRAYCTPPGHPSKSVISLALREYLQEQASNNSTRSLAEFKRLVAPQLRVFLFAGRNTTSS
TLIYTYYLLAQHPEALAKIRAEHGDVLGADPAEAQGRIKEDVQLLNKLPYTTAVIKETLRLFPPSASMRE
GRPDAEIIGEDGQRYPTVGCNVWTLTVALHHNSDYWDQVENFIPERWLVGPEDPLYPVKGAWRAFEFGPR
SCIGQTLAMLELRIALAMTIRQFDITPAYDEWDSIHPATTAREVNGHRAYQAERGAGGAHPADGFPCRVK
ERC
CYP59A5 Aspergillus oryzae
GenEMBL BAE48804.1
92% to 59A4, 71% to 59A1
MPEFKLLAGHFGTLKKTIQGMPSDATLHSIMLKISKQFQSGIFYINMWPFSGTWMIVSTPSAATQIQKLN
LTKPAILRQPPETVTGGPSMMTMHGETWKKWRALFNPGFNPAYIIGLAPNITDEVATFCAQLRKKAQQGE
VFPLESLTTRLTVNSICSVVLDTQLHHQIKDYPLATALQRQINWTSFGTTFNPLKRYFTIRPLVLWYNNK
VMDRIIGGEVDRAYRTPPDHPSKSVISLALREYLQEQASSNSTRSLAEFKRLVAPQLRVFLFAGRNTTSS
TLIYTYYLLAQHPDILAKIRAEHEDVLGVNPEEVQGRIKEDAQLLNKLPYTTAVIKETLRLFPPSASMRE
GRPDAEIIGENGQRYPTVGCNVWTLTVALHHNSDHWNQVESFTPERWLVGPEDPLYPVKGAWRAFEFGPR
SCIGQTLAMLELRIALAMTIRQFDITPAYDEWDSIHPATTSKEVNGHRAYQAERGAGGAHPADGFPCRVK
ERF
CYP59A5 Aspergillus flavus
AFL2G_07219
96% to CYP59A5 Aspergillus oryzae
see Fungal pages for seq
CYP59A6 Aspergillus nomius isolate AN13137
AY510454.1
join(49221..49702,49768..50767)
94% to CYP59A5 Aspergillus flavus
gene VerA of aflatoxin biosynthesis
MPEFKLLAGHFGTLKKTIQGMPSDATLHSIMLKISQQFPSGIFY
INMWPFSGTWMIVSAPSAASQIQKLNLTKPAILRRPLETVTGGPSMMSMHGETWKKWR
ALFNPGFNPAYIIGLAPNIADEVATFCTQLRKKAQQAEVFPLESLTTRLTVDTICSVV
LDTQLHHQIKDHPLATALQRQIDWTSFGTTFNPVKRYLTIRPLVLWYNNKVMDRIIDS
EVDRAYRTPPDRPSKSVISLALREYLKEQSSSSSTRSLAEFKRLVAPQLRVFLFAGRN
TTSSTLIYTYYLLAQHPEVLARIRAEHEDVLGGDPADAQGRIKEDVQLLNKLTYTTAV
IKETLRLFPPSASMREGRPDAEIIGEDGQRYPTVGCNVWTLTVALHHNSDHWQQVESF
IPERWLVGPEDPLYPVKGAWRAFEFGPRSCIGQTLAMLELRIALAMTIRQFDITPAYD
EWDSIHPATTAREVNGHRAYQAERGAGGAHPADGFPCRVKERC*
CYP59A7 Aspergillus ochraceoroseus strain SRRC 1432
AY092402.3
join(9134..9615,9669..10671)
gene StcS/AflN in aflatoxin/sterigmatocystin biosynthesis
76% to CYP59A5 Aspergillus flavus
MPEFKLLAGHFAALKQTIQGMPPNATLHSIMLKLSQQFPSGIFY
INLWPFSGTWMIVATMTAASQIQSLNLTKPAILRRPLETITGGPSLISMHGETWKRWR
ALFNPGFNPAYLIGLAPNIADEVAVFRNQLRKKAQQGDIFSLEPLTLRLTVDTICSVA
LDARLFHQQKDHPLAVALQRQIEWASFGTTFNPVKRYLTIRPLVLWYNSRLMNRLIGK
EVDRVHNARPGYSSKSVISLALKQYLKEQSNGDTRQSLEAFKRQVAPQLRVFLFAGRD
TTSSTLLYTFYLLAKHPEILERVRREHDEVFGTDIKKAQSCIAEDPQLLNKLPYTLAV
IKETLRLFPPSASMREGRPDVEIVGDDGRRYPTAGCNVWTLTVALHHNSNHWPEVESF
IPERWLVGHEDPLYPAKGAWRAFEFGPHSCIGQTLALLELRIALAMTLREFNITPAYD
EWDRLHPRDTIKEVNGHRAYQMEKGGGGAHPADGLPCRVTLRTT*
CYP59B1X Fusarium graminearum (Gibberella zeae PH-1)
GenEMBL AACM01000117.1
52362-54017 (+) strand, one intron
renamed CYP5071A1
CYP59C1 Aspergillus nidulans
AN5766.2
44% to 59A1 586clan?
Revised 7/18/07
MAVTNLVLTLSIPLLTVIGYGLYCGFQHRRKINELRKRGIPMPKDWSWLT
GHLLSLKKYTDRLPADAHVLLPTHELAVEFADTEMFLMDTWPVFPALVMV
YDPDAALQISTKYNLPKSAIFPSLMHPITGGPSMISMNDAEWKKWRSIFN
PGFSAGNMVDQVSTVVDSVQVFCDILREKAGTGLVHLDDLTTRLTMEVIL
KVTLDMDSNYQRSDNEMVHALNTITKWHSFWDPRVRANPLRPLVQKYYGR
VMDRCIRKELDKRFAEMQQGQRSASTSKRVKSVIALALEAYLADYHQKDG
KASKLDDNFAQYATHQIRLFLFAGNDTTSSSIVYVYHMLSKHPKALARVR
QEHDRIFGTLPSAAPQLLKSNPALLNQCPYTLAVIKETLRLFPPASTTRE
GRDGVTLTDRLGNSYPLGHTIGAEIIHPTIHKNPRLWPQAEEFIPERWLV
DPGHELYPSPAAWRPFEHGPRNCIGQTLVYNEMRIVIVMTARTFNIRPAY
DEWDAMHAAKEGLL (0)
TVHGERAYQTEKAGTHPADGYPCHVALCSGSPIS*
CYP59C2 Aspergillus niger
e_gw1.2.1390.1|Aspni1
63% to CYP59C1
see Fungal pages for seq
CYP59D1 Aspergillus oryzae
GenEMBL BAE66084.1
AP007175.1
55% TO 59D2, 41% to 59A1, 41% to 59C1
470087 MVIAVSDLVGSYGARVALVLALVIVLRFLQEMLKVRLLFYRLRKQ
GLPMPKWNFAAGNLQMLPDLMKRHP
KGSQQSEAFTLLSYEFASSDNCFYIDVWPFTKPLLVVNSPDLAVQACQTYALPKPPVLAKFFNPFAGGPS
IFTTNGPEWKRNRGLFNPAFSTSNILQHTPHI 470671
VEEAEEYVEILREHARKGDTFTLDKMTCDYVLDIIGRVAM (2)
KARLHSQRGRNPVAAALRSQVEWHCQDEQMNPFIRWNPMRPIMQWCNGRTM
NQYIGAELDKRYEAWTQNKPSTRANSIIDIVLAEYMSTRPVRAALDPEFKSWAT
IQLRTFLFAGHDSTAATIVYSIYLLSKHPEILSKVRTEHDEVFGSDISAAAGILKQHPEL
INRLPYTLAVIKETLRLFPAASALREGQPGVYLQDKNGTKYPTEGLCIWIIHGGIQRNPN
YWPDPHAFKPERWLVGPDHPLYPPKGAWRPFEQGLRDCIGQALALLDVKITLVLTLREFD
FQDQYAHWDRLHPRSGPKTVFGERAYQIPQGGSHPVDGLPCRVSLRNQITK*
CYP59D1 Aspergillus flavus
AFL2G_11439
99% to CYP59D1 Aspergillus oryzae
see Fungal pages for seq
CYP59D2 Aspergillus oryzae
GenEMBL BAE58153.1, AP007155.1
37% to 59A1, 37% to 59C1
2825205 MGANTLGWDGLTSRIAVAVATVCLTSFVYKLIKMRLMFYRLRKKGL (0) 2825068
2825008 PTPPWNPILGNLAVMAQLQKKWPSDSREAESFALLSTEAPGCEAGFYVDVWPFSIP
MLVVTSPALAVQACQTYDLPKPDVLQPFINPMAGGSDNLFVSNGAHWKQARELFNHGFSMAAAMSHMTYI
LEEAQVFVQMLKDHARKGDTFSLDALTCRYVMDIIGNVA LNTRFRFQEQHNPIAAAMRDTIELECGIETS
NFLSRWNPRRLYRQWQNGRTMDYLIGVELDKRYKEWRETAKSSSHPRTQSIMDMVIAEYMKTRPQAQQQQ
ELDPEFKRWATIQIRLFLFVGHDSEATTIIYSLYLLSKNPGVLIKVRAEHDRVFGAGVSSAYDVLTDHPE
KINQLSYTHAVIKETLRLFPPANGLRGGLPGVSLRDEQGRIFPTEGCAIWIVHTAVHRNPSSWPQPHAFI
PDRWLVEPGHPLYPPAGGWRPFEQGPRNCIGQNISLLGIKASLAMLVRQFDFHDAYAEYDRLHPSTGLKT
MFGERAYMIQKGAGHPAQGFPCKVTLR
CYP59D2 Aspergillus flavus
99% to CYP59D2 Aspergillus oryzae
see Fungal pages for seq
CYP59D3 Aspergillus niger
e_gw1.12.714.1|Aspni1
63% to CYP59D2
see Fungal pages for seq
CYP59E1 Mycosphaerella graminicola
Mgr034
39% to 59C1, 9/10 top hits are CYP59, but only 34% to 59D3
N-term is short
see Fungal pages for seq
CYP59E2 Mycosphaerella fijiensis
e_gw1.7.674.1
61% to CYP59E1 Mycosphaerella graminicola
see Fungal pages for seq
60A Subfamily
CYP60A1 Aspergillus parasiticus
GenEMBL L40839 (1266bp) L41149 (1284bp)
Yu,J., Chang,P.-k., Cary,J.W., Wright,M., Bhatnagar,D.,
Cleveland,T.E.,
Payne,G.A. and Linz,J.E.
Comparative mapping of aflatoxin pathway gene clusters in
Aspergillus
parasiticus and aspergillus flavus.
Applied Environ. Microbiol. 61, 2365-2371 (1995)
gene ord1
missing N-terminal (approximately 93 amino acids missing)
CYP60A2 Aspergillus nidulans
GenEMBL U34740 (507 amino acids)
Brown, D.W., Keller, N.P. and Adam, T.H.
Proc. Natl. Acad. Sci. USA 93, 1418-1422 (1996)
gene stcF
CYP60A2 Aspergillus nidulans
U34740 AN7818.1
53 clan, note the CYP60 family falls inside the
much larger CYP65 family that has grown
stcF revised 7/19/07
MILPLILVLYLLSTAAYRLWLHPVRNYPGPCWWAVWRVPYLKGT
IRGTIVRDIQRLHNQYGPVVRIAPDELSYITPEAAKPIYTSSPEFPKDPMLLPPFHNG
APGILAADYAHHRRYRRLLASAFSEKGLRAQQGMIQSHIDRLMTRLQGNCSSGSLDMT
VWFNWATFDIIGDLAFGEPFGCLERMETNPWIASIQGNVKSIPILNALRRYRLDRLIE
FLAPPRLLEMRRRNAQFTAEKVDRRLKHATTTRGDLWDSVLADPPDGEPPMSRAEMVS
NASAIVLAGSETSATTLSGCLWLLLTNPEYLQQLTERIRARFSTATVIDAQTVTQIQG
LQAVLDESLRLYPAVPMQSNRIVPPPGARLAGSWVPGGTSVAVQQFAACRSPTNFHRP
DEFIPERWEKEGEFINDRREASQPFSIGPRNCIGRQLALAEMRLILVHLLWHFDIELD
RRRMENMDWMAVQGI
WILWDKKPLWVVLKNRST*
CYP60A3 Aspergillus parasiticus
GenEMBL U62774
Yu,J., Chang,P.K., Cary,J.W., Bhatnagar,D. and Cleveland,T.E.
avnA, a gene encoding a cytochrome P-450 monooxygenase, is involved
in the conversion of averantin to averufin in aflatoxin
biosynthesis in Aspergillus parasiticus.
Appl. Environ. Microbiol. 63, 1349-1356 (1997)
CYP60A3 Aspergillus parasiticus aflatoxin pathway gene cluster
GenEMBL AY371490
complement(join(46528..46863,46931..48025,48072..48128))
gene="aflG"
function="conversion of averantin to 5'-hydroxyaverantin
70% to CYP60A2
note CYP60A1 and CYP60A3 are essentially the same sequence
the CYP60A1 sequence is incomplete and has 4 amino acid differences.
CYP60A4v1X Aspergillus flavus strain SRRC 141
GenEMBL AF106960
Bhatnagar,D., Cary,J.W., Ehrlich,K.C., Cleveland,T.E. and
Payne,G.A.
Molecular characterization of an aflatoxin B2 producing mutant
strain of Aspergillus flavus
Unpublished
verB gene 85% identical to 60B2
note: error in name assignment, this gene is CYP60B4v1
MYFLSLPALAIVIPVGYVLFQLGYNLFFHPLRGYPGPLLWRASS
LPWKIALLRGTMHHDLMRYHQKYGDTVRIKPDEISYANAQAWRDIHAHVPGRPEFLKD
PVRLPLAPNGVMSILVSDTKNHARFRSLFGHAFSDKGLRTQESTIVQYADLLVEVLRE
VANTGRSAEMVYYFNMAIFDSIGALSFGESFDSLKSRQLHPWVDAIHKNLKSVAISHV
LRSMGIEFLTPYVLPKELRGKRQENYSYAVEKLNKRMKMEGDQGDFWDKVLVKSADDN
QRGDGMSAGEMLNNAAVMVVAGSETTASALSGAMYLLCLSGKIKKATAEIRKSFASPE
DIDLISVSHLPYLTAVIDETLRMYPAVPGQPPRVVPAGGATVCGRFVPEETRVGVSHL
GAYFADYNFTHADKFIPERHLQKTEEPYKYDNYGAYQPWSVGLRNCIGRNLAYAEVRL
TLAKLLWHFDFTLDVGKTGNFLDQKIWSIWAKRELYMFMKTRGTSSSSPQ
CYP60A4v2X Aspergillus flavus strain SRRC 1007
GenEMBL AF106959
Bhatnagar,D., Cary,J.W., Ehrlich,K.C., Cleveland,T.E. and
Payne,G.A.
Molecular characterization of an aflatoxin B2 producing mutant
strain of Aspergillus flavus
Unpublished
verB gene
99% identical to 60A4v1
note: error in name assignment, this gene is CYP60B4v2
CYP60A4v3X Aspergillus parasiticus strain RH1
GenEMBL AF106958
Bhatnagar,D., Cary,J.W., Ehrlich,K.C., Cleveland,T.E. and
Payne,G.A.
Molecular characterization of an aflatoxin B2 producing mutant
strain of Aspergillus flavus
Unpublished
verB gene
97% identical to 60A4v1
note: error in name assignment, this gene is CYP60B4v3
CYP60A4v3X Aspergillus parasiticus aflatoxin pathway gene cluster
GenEMBL AY371490
complement(join(48496..48846,48901..50052))
gene="aflL"
function="conversion of versicolorin B to versicolorin A"
note: error in name assignment, this gene is CYP60B4v3
CYP60A5 Aspergillus oryzae
GenEMBL BAE71325.1, BAC45242.1, BAE59521.1
95% to CYP60A3
MGGDGWPSDGHILLLIVLTVLTPPSLALYRLWIHPLRSYPGPRWWAIWRGPYILSNIRGNLVRDLQRLHQ
QFGHVVRIAPNELSFIVPEAASPIYTSNPEFPKDPMHLPPFHNGTPGILAADHAHHRRYRRLLAFSFSDK
GLRQQRSLIERSVNLLITRLHENCGQGPLDLTLWFNWATFDIIGDLAFGNSFGCLDNVQTHPWISSIQGN
VKLIPILNAFRRYRLDGLLQLLGSRKLLEQRRRNAQFTTDQVDRRLKNSSTPRGDIWDAVLAQKPDGEPP
MSREEMISNTSAIVLAGSETSATLLSGCTWLLLKNPGHLHQLTSRIRSQFTHASEIDSQSVSRVEGLQAI
LEESLRLYPPVPMQSNRIVPQSGAYIAGGWVPGGTSVGLQQFVACRSSSNFHRPEEFLPERWQGQGEFAH
DRREVSQPFSIGPRNCIGRQLAYVETRLILVKLLWHFDLRLDTTRMKDTDWLAEQGIWILWDKNPLWVNL
EPRNE
CYP60A5 Aspergillus flavus
AFL2G_07217 revised
98% to CYP60A5 Aspergillus oryzae
Bottom HALF (two adjacent P450s in this gene model CYP60B3 and CYP60A5)
see Fungal pages for seq
CYP60A6 Aspergillus nomius isolate AN13137
AY510454.1
complement(join(51845..52180,52258..53352,53400..53456))
92% to CYP60A3
gene AvnA of aflatoxin biosynthesis
MGVDGWLSDGHFRLLILLIVLTPPSLAVYRLWIHPLRSYPGPRW
WAIWRGPYILSNTRGSLVRDLQRLHQQFGPVVRIAPNELSFIAPEAAAPIYTSNPEFS
KDPMHLPPFHNGTPGILAADHAHHRRYRRLLAFSFSDKGLRQQRGLIERSVNLLITQF
HENCGQGPLDLTLWFNWATFDIIGDLAFGDSFGCLDNVQTHPWIASIQGNVKLIPILN
GLRRYRLDGLLRLLGSRKLLEQRRRNAQFTTDQVDRRLQNSSTPRGDIWDAVLAQKLD
GEPPMSRAEMISNASAIVLAGSETSATLLSGCTWLLLKNPEHLHQLTSRIRSEFSHAS
EIDSQSVSRVEGLQAVLEESLRLYPPVPMQSNRIVPSTGAHIAGGWVPGGTSVGLQQF
VACRSPSNFHRPDEFLPERWQGQGEFAHDRREVSQPFSIGPRNCIGRQLAYVEMRLIL
VKLLWHFDLRLDTTRMKGTDWLAEQGIWILWDKKPLWVTLEPRDK
CYP60A7 Aspergillus ochraceoroseus strain SRRC 1432
AY092402.3
complement(join(29656..30030,30172..31266,31338..31385))
gene AflG in aflatoxin/sterigmatocystin biosynthesis
79% to CYP60A3 Aspergillus parasiticus avnA
gene model revised at one intron boundary
MDVLRSFPLQLLGLRDFLILGSLGAYRLWLHPLRSYPGPRWWAL
WRVPYLQSTIRGTIVRDLQRLHEQYGPVVRIAPDELSFINPEAAKPIYTSNPEFSKDP
MHLPPFHNGVPGILAADHAHHRRYRRLLAFSFSDKGLKAQQGMIQQYIDQLIVRLHEN
CSTGPVDMTLWYNWATFDIIGDLAFGESFGCLEKVETHPWIASIQGNVKAIPILNALR
RYRLSGVLGLLAPRNLLEMRRRNAQFTADKVDRRLQHPGTARGDLWDSVLADNPNGES
PMSRDEMVSNASAIVLAGSETSATLLSGCTWLLLKNPEYLNTLTERVRSQFARAADID
AQTVTQVEGLQAVLEESLRLYPPVPMQSNRIVPPQGAQIAGMWVPGG (0)
TSVAVQQFVACRSADNFHRPNEFLPQRWQGQGEFANDRREASQPFSIGPRNCIGRQLAYAEMRL
ILVHVLWHFNLRLDQGRMKDADWLAEQGIWILWDKNHCGLCWNRGREKVEEDDAT*
60B Subfamily
CYP60B1 Aspergillus nidulans
GenEMBL U34740 (501 amino acids) AN7813.2, revised 7/18/07
Brown, D.W., Keller, N.P. and Adam, T.H.
Proc. Natl. Acad. Sci. USA 93, 1418-1422 (1996)
gene stcL
MAFLSLPILTALGA
VVYVLFQLVYNLYFHPLRDYPGPLLW
RASSLPWKLTLLRGTMHHDLMRHHQTYGDTVRIKPDEISYANGQAWRDIHAHVPGRPE
FLKDPVRLPLAPNGVMSILVSDTRNHARFRSLFGHAFSDKGLRAQEPTIARYADLLVE
VLREVADTGKSVEMVRYFNMAIFDSIGALSFGESFDSLRNRELHPWVDTIHKNLKSVA
ISHVLRSMGVEFLAPYLMPAELRGKRQENYTYAIEKLKKRMQKTGDQGDFWDRVIVKS
ADGNQSGDGMSYGEMINNAAVMVVAGSETTSSALCGCTYLLCKFDKMDKAVAEVRGAF
AAADQIDLVSVSRLPYLTAVIDETLRMYPSVPGQPPRVVPEGGAIVCGRFVPAETRVG
VSHLGAYYAPYNFSHADKFIPERHLAGAKLEEPFRHDNYAAYQPWSVGVRNCIGRNLA
YAEVRLTLAKLLWHFDISLDEERTGNFLDQKIWSIWAKRELYLEIRTREF
CYP60B2 Aspergillus ochraceoroseus strain SRRC 1432
GenEMBL AY092402.3
complement(join(46781..47113,47181..48332))
Cary,J.W., Beltz,S.B., Bennett,C.A. and Klich,M.A.
Molecular Characterization of the Aflatoxin Biosynthetic Cluster
from Aspergillus ochraceoroseus.
Unpublished
function="desaturation of aflatoxin precursor VER B to VER A
84% to CYP60B1 Aspergillus nidulans StcL
probable ortholog of CYP60B1
gene AflL/StcL
MALPTLPVLAVLIGASYILVQLVYNLYFHPLRDYPGPLLWRASS
LPWKFTLLRGTMHHDLMRFHQKYGDTVRIKPDEISFANAQAWRDIHAHVPGRPEFLKD
PVRLPLAPIGVMSILVSVTRFLARFRSVFGLAFSVKGLRAQEPTIVQYADLLVEVLRE
VADTGKSVEMVHYFNMAIFDSIGALSFGESFDSLKNRQLHPWVDAIHKNLKSVAISHV
LRSMGIEFLTPYVLPKELRGKRQENYTYAIEKLKRRMQMTGDQGDFWDRVIVKSADGN
QSGDGMSPGEMLNNAAVMVVAGSETTSSALCGTTYLLCQSGKMEKAVAEIRNAFPTPD
KIDLVTVSHLPYLTAVIDETLRMYPAVPGQPPRVVPAGGATVCGKFLPAETRVGVSHL
GAYYADYNFTRPEKFIPERHLQKMEEPFKHDNYGAYQPWSVGVRNCIGRNLAYAEVRL
TLAKLLWHFDITLDEAKTGNFLDQKIWSIWANGELYMSFKARKV
CYP60B3 Aspergillus oryzae
GenEMBL BAE71326.1, BAC45243.1, BAE59522.1
81% TO 60B1
probable ortholog of CYP60B1
MYFLSLPALAIIVPVGYVLLHLGYNLFFHPLRGYPGPLLWRASSLPWKIALLRGTMHHDLMRFHQKYGDT
VRVKPDEISYANAQAWRDIHAHVPGRPEFLKDPVRLPLAPNGVMSILVSDTKNHARFRSLFGHAFSDKGL
RTQESTIVQYADLLVEVLREMANTGRSAEMVYYFNMAIFDSIGALSFGESFDSLKSRQLHPWVDAIHKNL
KSVAISHVLRSMGIEFLTPYVLPKELRGKRQENYSYAVEKLNKRMKMEGDLGDFWDKVLVKSADDNQRGD
GMSAGEMLNNAAVMVVAGSETTASALSGAMYLLCLSGKIEKATAEIRKSFASPEDIDLISVSHLPYLAAV
IDETLRMYPAVPGQPPRVVPAGGATVCGRFVPEETRVGVSHLGAYFADYNFTHADKFIPERHLQKTEEPY
KYDNYGAYQPWSVGLRNCIGRNLAYAEVRLTLAKLLWHFDFTLDVGKTGNFLDQKIWSIWAKRELYMFIK
TRGTSSSSPQ
CYP60B3v1 Aspergillus flavus
AFL2G_07217 revised
97% to CYP60B3 Aspergillus oryzae
TOP HALF (two P450s in this gene model CYP60B3 and CYP60A5
see Fungal pages for seq
CYP60B3v2 Aspergillus flavus strain SRRC 141
GenEMBL AF106960
Bhatnagar,D., Cary,J.W., Ehrlich,K.C., Cleveland,T.E. and
Payne,G.A.
Molecular characterization of an aflatoxin B2 producing mutant
strain of Aspergillus flavus
Unpublished
verB gene 85% identical to 60B2
97% to 60B3v1 ortholog
99% to CYP60B3v3 Aspergillus flavus strain SRRC 1007
formerly CYP60B4v1
MYFLSLPALAIVIPVGYVLFQLGYNLFFHPLRGYPGPLLWRASS
LPWKIALLRGTMHHDLMRYHQKYGDTVRIKPDEISYANAQAWRDIHAHVPGRPEFLKD
PVRLPLAPNGVMSILVSDTKNHARFRSLFGHAFSDKGLRTQESTIVQYADLLVEVLRE
VANTGRSAEMVYYFNMAIFDSIGALSFGESFDSLKSRQLHPWVDAIHKNLKSVAISHV
LRSMGIEFLTPYVLPKELRGKRQENYSYAVEKLNKRMKMEGDQGDFWDKVLVKSADDN
QRGDGMSAGEMLNNAAVMVVAGSETTASALSGAMYLLCLSGKIKKATAEIRKSFASPE
DIDLISVSHLPYLTAVIDETLRMYPAVPGQPPRVVPAGGATVCGRFVPEETRVGVSHL
GAYFADYNFTHADKFIPERHLQKTEEPYKYDNYGAYQPWSVGLRNCIGRNLAYAEVRL
TLAKLLWHFDFTLDVGKTGNFLDQKIWSIWAKRELYMFMKTRGTSSSSPQ
CYP60B3v3 Aspergillus flavus strain SRRC 1007
GenEMBL AF106959
formerly CYP60B4v2
MYFLSLPALAIVIPVGYVLFQLGYNLFFHPLRGYPGPLLWRASS
LPWKIALLRGTMHHDLMRYHQKYGDTVRIKPDEISYANAQAWRDIHAHVPGRPEFLKD
PVRLPLAPNGVMSILVSDTKNHARFRSLFGHAFSDKGLRTQESTIVQYADLLVEVLRE
VANTGCSAEMVYYFNMAIFDSIGALSFGESFDSLKSRQLHPWVDAIHKNLKSVAISHV
LRSMGIEFLTPYVLPKELRGKRQENYSYAVEKLNKRMKMEGDQGDFWDKVLVKSADDN
QRGDGMSAGEMLNNAAVMVVAGSETTASALSGAMYLLCLSGKVEKATGEIRKSFASPE
DIDLISVSHLPYLTAVIDETLRMYPAVPGQPPRVVPAGGATVCGRFVPEETRVGVSHL
GAYFADYNFTHADKFIPERHLQKTEEPYKYDNYGAYQPWSVGLRNCIGRNLAYAEVRL
TLAKLLWHFDFTLDVGKTGNFLDQKIWSIWAKRELYMFIKTRGTSSSSPQ
CYP60B3 Aspergillus parasiticus strain RH1
GenEMBL AF106958
formerly CYP60B4v3
MYFLSLPSLVIVIPVGYLLFHLGYNLFFHPLRGYPGPLLWRASS
LPWKIALLRGTMHHDLMRFHQKYGDTVRIKPDEISYANAQAWRDIHAHVPGRPEFLKD
PVRLPLAPNGVMSILVSDTKNHARFRSLFGHAFSDKGLRTQESTIVQYADLLVEVLRE
VADTGRSAEMVYYFNMAIFDSIGALSFGESFDSLKSRQLHPWVDAIHKNLKSVAISHV
LRSMGIEFLTPYVLPKELRGKRQENYSYAVEKLNKRMKMEGDQGDFWDKVLVKSADDN
QRGDGMSAGEMLNNAAVMVVAGSETTASALSGAMYLLCLSGKIEKATAEIRKSFASPE
DIDLISVSHLPYLTAVIDETLRMYPAVPGQPPRVVPASGATVCGRFVPEETRVGVSHL
ATYFADYNFTHADKFIPERHLQKTEEPFKYDNYGAYQPWSVGLRNCIGRNLAYAEVRL
TLAKLLWHFDFTLDVDKTGNFLDQKIWSIWAKRELYMFIKTRGTSSSSPQ
CYP60B3 Aspergillus parasiticus aflatoxin pathway gene cluster
GenEMBL AY371490
formerly CYP60B4v3
MYFLSLPSLVIVIPVGYLLFHLGYNLFFHPLRGYPGPLLWRASS
LPWKIALLRGTMHHDLMRFHQKYGDTVRIKPDEISYANAQAWRDIHAHVPGRPEFLKD
PVRLPLAPNGVMSILVSDTKNHARFRSLFGHAFSDKGLRTQESTIVQYADLLVEVLRE
VADTGRSAEMVYYFNMAIFDSIGALSFGESFDSLKSRQLHPWVDAIHKNLKSVAISHV
LRSMGIEFLTPYVLPKELRGKRQENYSYAVEKLNKRMKMEGDQGDFWDKVLVKSADDN
QRGDGMSAGEMLNNAAVMVVAGSETTASALSGAMYLLCLSGKIEKATAEIRKSFASPE
DIDLISVSHLPYLTAVIDETLRMYPAVPGQPPRVVPASGATVCGRFVPEETRVGVSHL
ATYFADYNFTHADKFIPERHLQKTEEPFKYDNYGAYQPWSVGLRNCIGRNLAYAEVRL
TLAKLLWHFDFTLDVDKTGNFLDQKIWSIWAKRELYMFIKTRGTSSSSPQ
CYP60B3 Aspergillus nomius
AY510454
complement(join(53898..54266,54322..55473))
gene VerB of aflatoxin biosynthesis
94% to CYP60B3 Aspergillus flavus
MYFLSLSVLALVTLVSYVLFHLGYNLFLHPLRGYPGPLLWRASS
LPWKIALLRGTMHHDLMRYHQKYGDTVRIKPDEISYANAEAWRDIHAHVPGRPEFLKD
PVRLPLAPNGVMSILVSDTRNHARFRSLFGHAFSDKGLRTQESTILQYADLLVEVLRE
VADTGRSAEMVYYFNMAIFDSIGALSFGESFDSLKSRQLHPWVDAIHKNLKSVAISHV
LRSMGIEFLTPYVLPKELRGKRQENYSYAVEKLNKRMRMEGDQGDFWDKVLVKSADDN
QRGDGMSAGEMLNNAAVMVVAGSETTASALSGSMYLLCLSGKIEKATAEIRKSFASPE
EIDLISVSHLPYLTAVIDETLRMYPAVPGQPPRVVPAGGATVCGKFVPEETRVGVSHL
GAYFADYNFTHADKFIPERHLQKTEEPFKYDNYGAYQPWSVGIRNCIGRNLAYAEVRL
TLAKILWHFDFTLDVDKTGNFLDQKIWSIWAKRELYMTLKSRDTSCSWPSTSSSPQ*
CYP60B4v1X Aspergillus flavus strain SRRC 141
GenEMBL AF106960
Bhatnagar,D., Cary,J.W., Ehrlich,K.C., Cleveland,T.E. and
Payne,G.A.
Molecular characterization of an aflatoxin B2 producing mutant
strain of Aspergillus flavus
Unpublished
verB gene 85% identical to 60B2
note: error in name assignment, this gene was erroneously called
CYP60A4v1
probable ortholog of CYP60B1
note: Asp. oryzae and Asp. flavus are very closely related species
This seq is 97% identical to CYP60B3
Their P450 genes are nearly identical
So this is the ortholog/ same gene as 60B3v2
MYFLSLPALAIVIPVGYVLFQLGYNLFFHPLRGYPGPLLWRASS
LPWKIALLRGTMHHDLMRYHQKYGDTVRIKPDEISYANAQAWRDIHAHVPGRPEFLKD
PVRLPLAPNGVMSILVSDTKNHARFRSLFGHAFSDKGLRTQESTIVQYADLLVEVLRE
VANTGRSAEMVYYFNMAIFDSIGALSFGESFDSLKSRQLHPWVDAIHKNLKSVAISHV
LRSMGIEFLTPYVLPKELRGKRQENYSYAVEKLNKRMKMEGDQGDFWDKVLVKSADDN
QRGDGMSAGEMLNNAAVMVVAGSETTASALSGAMYLLCLSGKIKKATAEIRKSFASPE
DIDLISVSHLPYLTAVIDETLRMYPAVPGQPPRVVPAGGATVCGRFVPEETRVGVSHL
GAYFADYNFTHADKFIPERHLQKTEEPYKYDNYGAYQPWSVGLRNCIGRNLAYAEVRL
TLAKLLWHFDFTLDVGKTGNFLDQKIWSIWAKRELYMFMKTRGTSSSSPQ
CYP60B4v2X Aspergillus flavus strain SRRC 1007
GenEMBL AF106959
Bhatnagar,D., Cary,J.W., Ehrlich,K.C., Cleveland,T.E. and
Payne,G.A.
Molecular characterization of an aflatoxin B2 producing mutant
strain of Aspergillus flavus
Unpublished
verB gene
99% identical to 60B4v1
note: error in name assignment, this gene was erroneously called
CYP60A4v2
probable ortholog of CYP60B1
renamed CYP60B3v3
CYP60B4v3X Aspergillus parasiticus strain RH1
GenEMBL AF106958
Bhatnagar,D., Cary,J.W., Ehrlich,K.C., Cleveland,T.E. and
Payne,G.A.
Molecular characterization of an aflatoxin B2 producing mutant
strain of Aspergillus flavus
Unpublished
verB gene
97% identical to 60B4v1
note: error in name assignment, this gene was erroneously called
CYP60A4v3
probable ortholog of CYP60B1
renamed CYP60B3
CYP60B4v3X Aspergillus parasiticus aflatoxin pathway gene cluster
GenEMBL AY371490
complement(join(48496..48846,48901..50052))
gene="aflL"
function="conversion of versicolorin B to versicolorin A"
note: error in name assignment, this gene was erroneously called
CYP60A4v4
probable ortholog of CYP60B1
renamed CYP60B3
61A Subfamily
Note: all fungi have CYP61 a C-22 sterol desaturase in the ergosterol
biosynthesis pathway. These will just be called CYP61 without subfamilies
assigned. A few older names were 61A1-61A5. The sequential numbers may not
be needed, except in rare cases where there are more than one CYP61 in a single
species.
CYP61A1 Saccharomyces cerevisiae (yeast)
GenEMBL Z49211 (30,011bp) PIR S54015
Lye,G. and Churcher,C.M.
Unpublished 1995
Barrell,B., Rajandream,M.A. and Walsh,S.V.
S.cerevisiae chromosome XIII cosmid 9711 complement of 3155-4771.
CYP61A1 Saccharomyces paradoxus
98% to CYP61A1 S. cerevisiae
See the fungal pages for seq
CYP61A1 Saccharomyces mikatae
97% to CYP61A1 S. cerevisiae
See the fungal pages for seq
CYP61A1 Saccharomyces castellii
83% to CYP61A1 S. cerevisiae
See the fungal pages for seq
CYP61A1 Saccharomyces bayanus
93% to CYP61A1 S. cerevisiae
See the fungal pages for seq
CYP61A1 Saccharomyces kluyveri
79% to CYP61A1 S. cerevisiae
See the fungal pages for seq
CYP61A1 Kluyveromyces waltii
82% to CYP61A1 S. cerevisiae C-term part only
See the fungal pages for seq
CYP61A1 Yarrowia lipolytica
GenPept CAG84120.1
64% to CYP61A1 Saccharomyces paradoxus
See the fungal pages for seq
CYP61A1 Ashbya gossypii ATCC 10895
NP_982999.1
75% to CYP61A1 S. cerevisiae
See the fungal pages for seq
CYP61A1 Pichia stipitis
JGI model estExt_genewise1_human.C_chr_6.10410
82% to CYP61A1 Debaryomyces hansenii
See the fungal pages for seq
CYP61A1 Debaryomyces hansenii
CAG86871.1
80% to CYP61A1 Candida lusitaniae
See the fungal pages for seq
CYP61A1 Lodderomyces elongisporus
84% to CYP61A2 C.albicans
CYP61A2/61A1 C.albicans
GenEMBL AL033396 comp(39932..41485)
gene="Ca35A5.10c" 68.9% identical to 61A1
note: most fungi have onlt one CYP61 gene
so this is probably the ortholog of CYP61A1
but for historical reasons it was named CYP61A2
CYP61A1 Candida guilliermondii
79% to CYP61A1 Candida dublinensis
See the fungal pages for seq
CYP61A1 Candida parapsilosis
79% to CYP61A2 C.albicans
See the fungal pages for seq
CYP61A1 Candida lusitaniae
78% to CYP61A2 C.albicans
See the fungal pages for seq
CYP61A1 Candida dublinensis
96% to CYP61A2 C.albicans
See the fungal pages for seq
CYP61A1 Candida glabrata CBS138
GenPept CAG62657.1
81% to CYP61A1 S. cerevisiae
See the fungal pages for seq
CYP61A1 Kluyveromyces polysporus
86% to CYP61A1 S. cerevisiae
See the fungal pages for seq
CYP61A1 Kluyveromyces lactis
80% to CYP61A1 Saccharomyces kluyveri
See the fungal pages for seq
CYP61A1 Uncinocarpus reesii
75% to CYP61A1 Aspergillus fumigatus
See the fungal pages for seq
CYP61A1 Histoplasma capsulatum G217B
77% to CYP61A1 Uncinocarpus reesii
ABBT01000205.1 Ajellomyces capsulatus G217B 1837477-1839379 (+)
ABBT01000210.1 Ajellomyces capsulatus G217B 225529- 227431 (+)
Two identical sequences. Note 51F2 also has two identical sequences
One of them is also on ABBT01000205.1 and
the other is on ABBT01000207.1
See the fungal pages for seq
CYP61A1 Coccidioides immitis
90% to CYP61A1 Uncinocarpus reesii
See the fungal pages for seq
CYP61A1 Fusarium graminearum
FG01959.1 AACM01000104 FGcontig1.104_scaffold1
See the fungal pages for seq
CYP61A1 Nectria haematococca (Fusarium solani group)
88% to CYP61A1 Fusarium graminearum
See the fungal pages for seq
CYP61A1 Magnaporthe grisea
See the fungal pages for seq
CYP61A1 Ustilago maydis
GenEMBL XM_397965.1
in the 61 clan 49% to CYP61 N.crassa
See the fungal pages for seq
CYP61A1 Sporobolomyces roseus
JGI gene model estExt_fgenesh1_pm.C_40050
57% to CYP61A1 Ustilago maydis,
See the fungal pages for seq
CYP61A1 Malassezia globosa
74% to CYP61A1 Ustilago maydis
See the fungal pages for seq
CYP61A1 Aspergillus nidulans
AN4042.1
66% to 61A1 51 clan revised 7/19/07
MAEINGSFVSPAADATVFPQVFQPAGLIADFLNGLTLWKTLATLFALAVV
YDQFRYIYLKGAIVGPAWKLPFMGPFLQSVNPKFHEYKAKWDSGELSCVS
VFHKFVVIASTRDMSRKIFNSPTYVKPCVVDAAHKLLGKTNWVFLDGKEH
VDFRKGLNNLFTRQALSCYLPRMEEVYNDYYARFLKKSKNNNYKPTPWMP
EFRDLMCAVSCRTFVGHYISDEAIDKISVDYYNITAALELVNFPIILPFT
KTWYGKKAADMVLDEFAKCAAKSRARMAAGGEISCIMDAWIKAQLDSAKY
REKIAKGIEVDSSEKPPQVLRDFTDYEVSQTIFTFLFASQDATSSACTWL
FQLMADRPEILDKVREENLRLRNGDVNAPLTMDLLDSMTYTRAVVKETLR
YRPPVIMVPYIAKKDFPITDKITVAKGSMIIPSVYPATRDEEAYPNADSF
DPDRWITGTAEQHPKNFLIFGTGPHYCLGQTYAVLNLMAMIGKASMEMDW
VHTPTPQSEEIKVFATIFP (0)
DDCLLTFRPRA
CYP61A1 Aspergillus oryzae
GenEMBL AP007155.1, BAE58068.1
MANVTGSFVSPSADATVVPQLFQPSGLLGSLLGDFNVWKGLLTLFIAAVIYDQ (0?)
FRYFYQKGSIVGPRWKLPFMGPFLQSVNPKFHEYKAKWDSGELSCVSVFHK (2?)
FVVIASTRDMSRKIFNSPAYVKPCVVDSAHKLLGEDNWVFLDGKDHVEFRKGLNGL
FTRSALSCYLPRQEETFNQYFKHFLEKSKANGYKPTPWMPEFRELMTAISCRTFVGHY
MTDEVIQKINDDYYLITAALELVNFPIILPFTKTWYGKKAADMVMEEFAKCAAKSKAR
MAAGGEVSCIMDAWVKAQQVSAKYREDVAKGIPAEKPPQLLRDFTDEEIAKTVFTFLF
ASQDATSAASTWLFQLMADRPEVLEKVREENVRLRNGDINAPITMELLDQMEYTRAVV
KETLRYRPPVIMVPYLVKKDFPITEKITVLKGSMIIPSVWPATHDEEAYPNPDTFDPD
RWITGTAEQNPKNWLVFGTGPHYCLGQTYAQLNLMAMIGKASMEMTWEHTTTPKSEDI
KVFATIFPQ (0?)
DDCLLTFRPRA
CYP61A1 Aspergillus flavus
100% to CYP61A1 Aspergillus oryzae
CYP61A1 Aspergillus fumigatus Af293
GenEMBL XP_750145.1
cytochrome P450 sterol C-22 desaturase
81% to 61A1 Aspergillus oryzae
MANVNGSFVSPSADATISPQLFYNVDSLSAVLNGFTFWKALATLFFAAVIYDQLRYFYLKGSLVGPTFKL
PFMGPFLQSVNPKFHEYKAKWDSGELSCVSVFHKFVVIASTRDMSRKIFNSPAYVKPCVVDIAHKLLGPD
NWVFLDGKEHVEFRKGLNGLFTRSALSSYLPVMEECYNKYYKYFLEKSKANDYKPEPWMPEFRELMCAVS
CRTFVGHYMTDAAIKKIADDYYMITAALELVNFPFILPFTKAWYGKKASDMVLEEFSNCAAKSKAHMAAG
GEITCIMDAWVKAQQDSAKYNEKIAKGLPVEDSEKPSHLLREFTDYEIAQTVFTLLFASQDATSAACTWL
FQLVADRPDVLEKIREENLRVRNGNINAPLTMDLLDEMKYTRAVVRETLRYRPPVIMVPYLVKKDFPITD
SITVSKGSMIIPSVWPATHDPEAYPNPDSFDPDRWITGDAEKQAKNFLVFGTGPHYCLGQTYAQLNLIAM
IGKASLEMDWEHAPTPKSEDIKVFATIFPEV
CYP61A1 Neosartorya fischeri
99% to CYP61A1 Aspergillus fumigatus = ortholog
See the fungal pages for seq
CYP61A1 Gibberella moniliformis 7600
AAIM02000091.1
95% TO FUSARIUM GRAMINEARUM 61A1
99% to 61A1 F. oxysporum
also called Fusarium verticillioides FVEG_07284
MEAVNATSSGFSSVLAGTKYVNVALPPQVEYVIEAVSNAGVWTWVFTFIALCVAYDQ (1)
350695 IAYIIRKGPIEGPAMKLPFIGPFLDSMDPRFDGYHAKWSSGPLSCVSIFHK (2) 350847
350776 FVVIASTRDMARKVFNSPAYVKPTVVDVAPKLLGHDNWVFLDGKAHVDF 351054
351055 RKGLNGLFTRKALESYLPGQEEAYNTYFKHFLKMTKDAGGKPVPFMHEFREVMCAVSCRT 351234
351235 FVGHYISDEAVTKIAEDYYLITAALELVNLPVILPYTKSWYGKKAADMVLAEFSKCAA 351408
351409 KSKVRMAAGGEVTCIMDAWVLSMIQSERWREAEEKGEGHTVEKPAPLLRMFNDYEISQTI 351588
351589 FTFLFASQDATSSAATWLFQVTAQRPDVLDRVREENIKVRNGDPNAPITMDQLESLTYTR 351768
351769 AVVRELLRWRPPVIMVPYVTKKAFPLTENYTVPK (1)
GSMLI 351948
351949 PTTFMALHDPEVYDNPSHFDPERYYSGDAEEKGSKNYLVFGTGPHYCLGQVYAQLNLALM 352128
352129 IGKASVMLDWKHHATPKSEEIKVFATIFPM 352218
DDCPLTFEERKW*
CYP61A1 Fusarium oxysporum
99% to 61A1 Gibberella moniliformis
See the fungal pages for seq
CYP61A1 Aspergillus clavatus NRRL
NZ_AAKD03000006.1
87% TO ASPERGILLUS FUMIGATUS CYP61A1, 52% TO CYP61A6 A. clavatus
468671 MANINGSFVSPSADATLSPQFFQSAEFLPAILNGFTIWKALVTLFIAAVIYDQ (1) 468513
LRYFYLKGSLVGPTFKLPFMGPFLQSVNPK 468315
468314 FHEYKAKWDSGELSCVSVFHK (2)
FVVIASTR 468135
468134 DMSRKIFNSPAYVKPCVVDVAHKLLGADNWVFLDGKDHVEFRKGLNGLFTRSALSCYLPE 467955
467954 MEECYNQYYKRFLKKSKANQYKPEPWMPEFRELMCAVSCRTFVGHYITDEAVQKIADDYY 467775
467774 MITAALELVNFPFILPFTKAWYGKKASDMVLQEFSNCAAKSKARMAAGGEISCIMDAWVK 467595
467594 SQLDSAKYREKIANGVPPEEAGKPSHLLRDFTDYEIAQTIFTFLFASQDATSAACTWLFQ 467415
467414 LMADRPEILDKVREENVRVRNGDITAPLSMDLLDQMTYTRAVVKETLRYRPPVIMVPYLV 467235
467234 KKDFPITDSVTVAKGSMIIPSVWPATHDPEAYPNPDSFDPDRWITGDADKHPKNWLVFGT 467055
467054 GPHYCLGQTYAQLNLMAMIGKASMEMDWVHTPTPESEDIKVFATIFPQ 466911
DDCHLTFRPRA*
CYP61A1 Aspergillus niger
JGI gene model estExt_GeneWisePlus.C_12230|Aspni1
84% to CYP61A1
See the fungal pages for seq
CYP61A1 Aspergillus terreus
87% TO CYP61A1 Aspergillus niger
70% to CYP61A1 Aspergillus terreus
55% to CYP61A6 Aspergillus terreus
ATEG_03815.1
MATVNGSFVSPSADATIVPQLFAPSGLIGSVLEGFTVWKALLTLFLAAVIYDQLRYFWLKGSIVGPAMKLPFMGPFLQSV
NPKFHEYKAKWDSGELSCVSVFHKFVVIASTRDMSRKIFNSPAYVKPCVVDAAHKLLGADNWVFLDGRDHVDFRKGLNGL
FTRQALSCYLPRMEEVYNDYYARFLKKSKDVNYKPVPWMPEFRELMCAVSCRTFVGHYITDEAVQKIADDYYLITAALEL
VNFPIILPYTKTWYGKKAADMVLEEFSKCAAKSKARMAAGGDISCIMDAWVKQQLDSARYRENVAKGVPVDENEKPAQVL
RDFTDYEIAQTIFTFLFASQDATSAACTWLFQLMADRPEVLDKVREENLRVRNGDVNAPLTMDVLESLTYTRAVVKETLR
YRPPVIMVPYLVKKDFPVTDKITVSKGSMIIPSVWPATHDEEAYPNADSFDPDRWITGTAEQQSKNWLVFGTGPHYCLGQ
NYATLNLMAMIGKASMEMNWEHTPTPQSEDIKVFATIFPQDDCLLTFRPRA
CYP61A1 Mycosphaerella graminicola
67% to CYP61A1 A. oryzae
See the fungal pages for seq
CYP61A1 Mycosphaerella fijiensis
JGI gene model estExt_Genewise1.C_20563
80% to CYP61A1 Mycosphaerella graminicola
See the fungal pages for seq
CYP61A1 Chaetomium globosum CBS 148.51
NZ_AAFU01000895.1 AAFU01000895.1
84% TO N. CRASSA 61A1
MAANATTSPLATIKYGAASVAPQLEYVIDYVSNASTLSILATILATLVVYDQ (1)
6856 FRYILNKGSIAGPAWKMPFIGPFLQSMNPKFEEYYAKWLSGPLSCVSVFHK (2) 7008
7135 FVVIASTRDMARKVFNSPAFVKPCVVDVAHKLLGADNWVFLDGKAHVEF 7215
7216 RKGLNGLFTRRALEIYLPGQEEVYNRYFKEFVATTKQAGGEPVPFMTHFREVITAVSCRT 7395
7396 FVGHYITDEAVKKIADDYYLITAALELVNFPIILPYTKTWYGKKAADMVLAEFAKCAA 7569
7570 KSKARMAAGGEPNCIMDAWIIQMIQSQRWRDAQAKGDTEGVEKPTHILRDFTDYEISQTV 7749
7750 FTFLFASQDATSSAATWLFQIMAQRPDVLDRVREENLNVRNGDINAAVNMDQLESMTYTR 7929
7930 AVVRELLRYRPPVLMVPYVVKKPFPITETYTAPK (1)
GSMVIPT 8109
8110 TYMALRDPEVYDRPDEFDPERYYTGDAEVKGMKNYLVFGTGPHYCLGQQYAQLNLALMVG 8289
8290 KASLLLDWKHHATPKSEEIKVFATIFPM 8373
DDCPLTFEDRKW*
CYP61A1 Phanerochaete chrysosporium (white rot fungus)
Scaffold_75 JGI gene model ug.78.18.1
62% to CYP61A1 Usilago maydis
the sequence GYVLQYCLVLYAMLTHRR seems to be an
insertion and it may need to be removed
MASSQAAFPSTLSDSSRHSTDSPAFIGLLPTGSWF
YTTAAILLSLLVIEQSVYRYKKRHLPGDKWTIPLIGKF
ADSMKPTMEGYMKQWNSGALSAISVFNV
RFIVMASTTEYARKILNSPTYAEPCLVHSAKQIILPDNW
VFLTGKEHVEYRRGLNLLFTRKAL GYVLQYCLVLYAMLTHRR SLYLGIQDVITRKHFAKW
LADAAKDPSAKPIMMTARELNMETSLRVFCGNHIPEHGAKEISDKYWMITVALELVNFPL
AIPGTKVYNAIQARKAAMKWLELAARKSKESVAAGNPPECMLEEWVTILNDPAYKGR
REFSDHEMAMVVFSFLFASQDAMSSGLIYGFQHLADHPEVLAKVREEQER
VRGGDYEKPLTLEMMDEMPYLRAMVKETLRVKPPVTMVPYKTTKAFPISQDYTVPSGSMV
IPSFYNSLHDPAVFPDPDRFMPERWLDPNGSANTNPRNYLVFGSGPHKCIGLEYAMMNIA
LVLANAAVLMNWEHELTPQSDKVQIIATLFPQDGCKLKFSPRQHA*
CYP61A1 Cryptococcus neoformans var. neoformans B-3501A chromosome 6
AAEY01000030
CNBF1100
ESTs gb|CF191501.1|CF191501,gb|CF188802.1|
CF188802, gb|CF194425.1|CF194425
EAL20298.1
61% to CYP61A1 Ustilago maydis
See the fungal pages for seq
CYP61A1 Rhizopus oryzae (Zygomycete fungus, Mucoromycotina)
65% to P450-8 = CYP61 C-22 sterol desaturase
53% to CYP61A3 S. pombe
P450-10_var1
See the fungal pages for seq
CYP61A3/61A1 S. pombe
GenEMBL Z98974 join(10345..10419,10514..12001)
gene="SPAC19A8.04" 51% identical to CYP61A1
note: most fungi have onlt one CYP61 gene
so this is probably the ortholog of CYP61A1
but for historical reasons it was named CYP61A3
CYP61A1 Schizosaccharomyces japonicus
80% to S. pombe CYP61A3 (misnamed, should be CYP61A1)
See the fungal pages for seq
CYP61A1 Schizosachharomyces octosporus
See the fungal pages for seq
CYP61A4/61A1 Botrytis cinerea (a plant-pathogenic fungus infecting over 200
plant species)
GenEMBL AL111744 AL111746
Bitton,F., Levis,C., Fortini,D., Pradier,J.M. and Brygoo,Y.
58% to S. pombe 61A3
note: most fungi have onlt one CYP61 gene
so this is probably the ortholog of CYP61A1
but for historical reasons it was named CYP61A4
CYP61A5/61A1 Neurospora crassa
No accession number
Neurospora crassa sequence contig 1.384 (supercontig 48)
note: most fungi have onlt one CYP61 gene
so this is probably the ortholog of CYP61A1
but for historical reasons it was named CYP61A5
4488 MASNATSFTSPLASAKLGTVAVPPQLEY
4404 VIDTISQASGWTIVFTLLAVLVAWDQ (0)
VKYVLNKGTIA 4225
4224 GPSWKIPFIGPFLESLDPKFEEYYAKWLSGPLSCVSVFH (2) 4108
4037 KFVVIASTRDMARKVFNSPSYVKPCVVDVAHKLLGADNWVFLDGPAHVEFRKGLNGLFTR 3858
3857 RALEIYLPGQEEVYNRYFKSFLEITKNAGGKPVPWMPHFREVITAVSCRSFVGHYISDEA 3678
3677 VKKIADDYYLITAALELVNFPIIIPYTKTWYGKKAADMVLAEFAKCAAKSKVRMAAGGEA 3498
3497 TCIMDAWIKQMIDSKKWREAVASGNTEGLEKPTHLLRDFTDYEISQTIFTFLFASQDATS 3318
3317 SAATWLFQIMAQRPDVLDRVREENLKVRNGDIHAPINMEQLESMTYTRAVVRELLRYRPP 3138
3137 VLMVPYLVKKAFPITEDYTIPKGSMVVPTTYMALRDPEVYPNPDYFDPERYYSGDAEAKG 2958
2957 AKNYLVFGVGPHYCLGQHYAQLNLALFVGKASLLLDWKHHPTPLSEEIKVFATIFPKVSL 2778
DRSCCSACRDFFLLTLYRMTVPSLSRSASGKQRGFWVWTSI*
CYP61A1 Neurospora discreta
JGI gene model estExt_fgenesh3_kg.C_120065
98% to CYP61A5 N. crassa ortholog of CYP61A1
See the fungal pages for seq
CYP61A6 Fusarium graminearum
A second CYP61 gene in Fusarium graminearum
CYP61A6 Nectria haematococca (Fusarium solani group)
79% to CYP61A6 Fusarium graminearum (ortholog)
only 56% to 61A1 F. graminearum
55% to CYP61A1 Nectria
See the fungal pages for seq
CYP61A6 Aspergillus oryzae
GenEMBL AP007155.1, BAE57461.1
73% to CYP61A6 Nectria haematococca
MDAKMATEIFNTTSAQPESPQSWAYKTSQLQGALPDFLAEWSAWQYVVTFLVGLVLYDQ (1?)
VLYLKRKGALPGPTFKIPLMGPFIQAIHPTFDGYLRQWASGPLSCVSIFHK (2?)
672882 FVVLASDRDLAHKVFKSPAYAEPCLVPVARDIIGHKAWVFLQGRDHAEYRRGLV 673043
673044 PLFTSRAIATYLPVQERVLDDYYNQFVAATKANQGKPMAFMTLFREINCALSCRTFFGDY 673223
673224 ISDDQVKKIADDFYLATDALDLVNIPLAMYVPFTKTWLGKRTADAVHRVFAECAARCKAN 673403
673404 MAKGATPTCIVDHWVLHMMESNRYRERVAAGETNLEKPKNMIREFTNEEISDTLFTFLFA 673583
673584 SQDASSSATTWLFQILAQRPDVLDKLREENLAARGGDRNKPFDLPMLESLTYTNAVIKEL 673763
673764 LRYKPPVILVPYLATKDFPVTPDYTVPKGSMIIPSCYPALHDPEAYPNPDVFDPERWISG 673943
673944 DAESKTKNWLVFGAGPHDCLARKYVPLSLAGMIGKASLELDWEHHPTPRSEEIRVFATLFPM (0) 674129
DGCNLVFKRRP*
CYP61A6 Aspergillus flavus NRRL3357
AAIH01000781.1
Only 1 aa diff to 61A6 of Asp. Oryzae,
73% to CYP61A6 Nectria haematococca
MDAKMATEIFNTTSAQPESPQSWAYKTSQLQGALPDFLAEWSAWQYVVTFLVGLVLYDQ
VLYLKRKGALPGPTFKIPLMGPFIQAIHPTFDGYLRQWASGPLSCVSIFHR (2)
6085 FVVLASDRDLAHKVFKSPAYAEPCLVPVARDIIGHKAWVFLQGRDHAEYRRGLV 5924
5923 PLFTSRAIATYLPVQERVLDDYYNQFVAATKANQGKPMAFMTLFREINCALSCRTFFGDY 5744
5743 ISDDQVKKIADDFYLATDALDLVNIPLAMYVPFTKTWLGKRTADAVHRVFAECAARCKAN 5564
5563 MAKGATPTCIVDHWVLHMMESNRYRERVAAGETNLEKPKNMIREFTNEEISDTLFTFLFA 5384
5383 SQDASSSATTWLFQILAQRPDVLDKLREENLAARGGDRNKPFDLPMLESLTYTNAVIKEL 5204
5203 LRYKPPVILVPYLATKDFPVTPDYTVPKGSMIIPSCYPALHDPEAYPNPDVFDPERWISG 5024
5023 DAESKTKNWLVFGAGPHDCLARKYVPLSLAGMIGKASLELDWEHHPTPRSEEIRVFATLF 4844
4843 PM (0) 4838
DGCNLVFKRRP*
CYP61A6 Gibberella moniliformis
GenEMBL AAIM01002518.1
81% to CYP61A6 Nectria haematococca
also called Fusarium verticillioides FVEG_08786
29769 MTSIVSPRHEAAISNASSTLKEDISNG
29688 NGLLSSYLNGYSNAQILLAILVVLIAYDQCMYLWRKGPIAGPAFKIPFMGPFIRALYPKF 29509
29508 DHYLAQWASGPLSCVSVFHK (2) 29456
29396 FVVLASDRDIAHKVFKSPTYAKPCIVPMAETLLRPNAWVFLQGKAHTEYRKGLNGLFVN 29220
29219 KALSTYLPVQEKVYDDYFGRFVAASEANKGKPMAFMRLFREINCALSCRTFFGDYISQDA 29040
29039 VEKIADDFYEVTAALELVNVPLSVYVPFTKCWKGKRTADAVLAEFAKCAAACKANMTSGA 28860
28859 EPRCIVDQWVLHMMESKKYNDRIAAGEEGVEKPRNLIREFTDDEIGQTMFTFLFASQDAS 28680
28679 SSATTWLFQVLAQRPDVLDRLREENLAVRNGNRHQPFELSMLESLPYTNAVIKELLRYRP 28500
28499 PVIFVPYEATKSFPVTPKYTVSKGTLIVPTCYPALHDPQAYPNPETFDPDRWITGDAESK 28320
28319 TKNWLVFGAGPHDCLARKYVPLTMAAMIGKASLELDWVHHATSRSEEIRVFATLFPE (0) 28149
28084 DECQLVFTKRE* 28049
CYP61A6 Trichoderma reesei
GenEMBL AAIL01000244.1
75% to CYP61A6 Nectria haematococca
MATIIEGSMPISFESTINATQHGTSGLVESLWSSCSAASNWQIAITALLVLVAYDQ (1)
10081 YLYISRKGSIAGPTFKIPLIGPFLQAIYPKFDAYLAQWASGPLSCVSVFHK (2) 9929
9869 FVVLASDRDLAHKVFKSPTFVRPCLVPMAINIMRPTAWVFLNGKAHAEFRRGLTGLFTN 9693
9692 KALSTYLPVQEKVYADYFNRFVEHSKANGGKPTKFMGWFREINCALSCRTFFGDYISQEA 9513
9512 VKRIADDFYLITDALELVNIPLSIHVPFTKCWRGKRTADAVLEEFTKCAAACKVNMASGA 9333
9332 QPTCIVDQWVQHMFESKRYNEAVAAGVEGLEKPTNLIREFSDEEIAQTLFTFLFASQDAS 9153
9152 SSATTWLFQILAQRPDVLDRLRAENLAARGGDKNRPFELEMLESLTYTNAVVKELLRYRP 8973
8972 PVIFVPYLALKPFPVTPDYTVPKNAMIIPSCYPALHDPAAYPNPDVFDPERWITGDAETK 8793
8792 TKNWLVFGAGAHDCLARRYVPLTMAAMIGKASLELDWVHHATERSEEIRVFATLFPM (0)8625
DECPLVFTRRE* 8517
CYP61A6 Aspergillus terreus NIH2624
GenEMBL AAJN01000026.1
76% to CYP61A6 Nectria haematococca
MNVKMQADMANSTQPDRQIWHVTTHWQGKVSDSFSGWSACQYIVTILLGVIFYDQ (1)
44968 VMYIKRKGSIAGPTFKMPLMGPFLQAINPKFEEYLAQWASGPLSCVSVFHK (2)45120
45171 FVVLASDRDLAHKVFKSPSYAEPCLVPIAKDILGPKAWVFLQGKAHAEYRRGLTPLFT 45344
45345 NNALATYLPVQDKVFATYFDKFVAASAANNGRPMEFMTMFREINCALSCRTFFGDYISQD 45524
45525 AVKKIADDFYLVTAALELVNIPLSMYVPYTKPWLGKRTADAVHAEFARCAAACKANMASG 45704
45705 AAPTCIVDHWVLHMMESNRYRERVAAGEANVEKPTNLIREFTNEEIGETLFTFLFASQDA 45884
45885 SSSATTWLFQVLAQRPDVLDRVRDENLAARGGDKSKPFDLPMLEGLTYTNAVVKELLRHR 46064
46065 PPVIFVPYLATKNFPVTPSYTVPKGSMIIPSCYPALHDPAVYPDPDVFEPERWISGDAES 46244
46245 KGKNWLVFGAGAHECLAKRYVPLSMAAMIGKAALELDWTHHATERSEEIRVFATLFPM (0) 46418
DGCQLVFRKRS* 46499
CYP61A6 Chaetomium globosum CBS
GenEMBL AAFU01000675.1 148.51
74% to CYP61A6 Nectria haematococca
796 MAADHVNASSSTPELLMSSSGLASGSSSWQFRDGVVGITAEWSAWQYVITLLLGLVVYDQ (1) 975
1031 VMYLYRKGSITGPRFKVPLTGPFIQALHPKFESYLAQWASGPLSCVSVFHK (2) 1183
1242 FVVLASDRELAHKVFKSPTYTEPCIVPLAKDILGHKAWVFLQGKAHAEYRRGLTPLFT 1415
1416 NKAMATYLPAQEKVFADYFDKFVAASQANVNQPLPFMTLFREINCALSCRTFFGDYISPA 1595
1596 AVKKIADDYYQATAALELVNVPFSIYIPFTKPWRGKRTADAVHAEFAKCAAACKRNMATG 1775
1776 AAPTCIVDHWVRHMFESNRYRERVAAGETDVEKPSNLIREFTDVEISETLFTFLFASQDA 1955
1956 SSSATTWLFQILAQRPDVLDRLREENLAARGGDRNKPFDLAMLESLTYTAAVVKELLRYR 2135
2136 PPVIFVPYLATKAFPITPDYTVPKGAMVIPSCYPALHDPKVYPNPDTFDPDRWITGDAES 2315
2316 KTKNWLVFGAGAHDCLARRYVPLTMAAMIGKAALELNWKHVATEKSEEIRVFATLFPM (0) 2489
DGAQLVFEKRP* 2579
CYP61A6 Aspergillus clavatus NRRL
GenEMBL AAKD02000011.1
77% to CYP61A6 Nectria haematococca
MDTEIANVSRAAAQSGSPQAWT
669433 PQPSSGQNTLLDCLAGWSTWQYLVTFLLGIVVYDQ (1)
669274 VMYLKRKGSIAGPAFKIPLMGPFLQAIHPKFDAYLAQWASGPLSCVSVFHK (2) 669122
669053 FVVLASDRDLAHKVFKSPSYAEPCIVPIAKDIIGHKSWVFLQGKAHAEYR 668904
668903 RGLTPLFTNKAMATYLPVQEKVFTDYFDRFVTASQANRGRPMAFMSMFREINCALSCRTF 668724
668723 FGDYISQKAVKRIADDFYLATAALELVNIPLSMYIPFTKTWLGKRTADAVHVEFAKCAAA 668544
668543 CKANMATGAKPTCIVDHWVLHMMESKRYHERVAAGETDVEKPTNLIREFTNEEIGETLFT 668364
668363 FLFASQDASSSATTWLFQILAQRPDVLDRLREENLAARGGDRNRPFDLPMLESLSYTNAV 668184
668183 IKELLRHRPPVIFVPYLATKNFPVTPDYTVPKGSMVIPSCWPALHDPDVYPNPEVFEPER 668004
668003 WISGDAESKTKNWLVFGAGPHECLAKRYVPLSMAAMIGKAALELEWTHHATEKSEEIRVF 667824
667823 ATLFPM (0)
DGCQLVFSKRV* 667698
CYP61A6 Fusarium oxysporum
96% to CYP61A6 Gibberella moniliformis
See the fungal pages for seq
CYP61A7 Aspergillus niger
JGI gene model estExt_fgenesh1_pm.C_40133|Aspni1
74% to CYP61A5
See the fungal pages for seq
CYP61A8 Rhizopus oryzae (Zygomycete fungus, Mucoromycotina)
93% to P450-10_var1
53% to CYP61A3 S. pombe in CYP61 family
See the fungal pages for seq
CYP61A9 Rhizopus oryzae (Zygomycete fungus, Mucoromycotina)
50% to CYP61A3 S. pombe = CYP61 C-22 sterol desaturase
See the fungal pages for seq
CYP61A10 Aspergillus terreus NIH2624
NW_001471411.1 AAJN01000002.1
72% TO 61A1 A. ORYZAE, 55% TO A. TERREUS 61A6
70% TO CYP61A1 Aspergillus niger
70% to CYP61A1 Aspergillus terreus
55% to CYP61A6 Aspergillus terreus
formerly CYP61A1, but a better match was found in A. terreus to CYP61A1
247642 MDFISKNTSPIIQKFQSPGIVAIFGSDISIWMVLLTVLITAVVYDQ (1) 247780
247843 FKYIRLKGTIAGPSWKIPFMGPFLESMDPKLEGYMAKWRSGELSCVSIFHK (2) 247997
248055 FVVIASTREMSCKIFNSPMFVKPCVVDSAHKLLGKDNWVFLDGKEHVDFRKGLNLLFTR 248228
248229 SALACYLPRQEEVFDMYFERFVARSAANNYKPLPWMPEFRKLMCTISCRTFVGHYISESA 248408
248409 LDKIAHDYYLITAALELVNFPIVLPFTKSWYGKRAADMVLREFSQCAAKSKLHIASGGEI 248588
248589 TCIMDAWVKAQLDSAAYRKNVAAGIPCEKPSHLLRDFTDYEIAQTVFTLLFASQDAN 248759
248760 SSAITWLFQIMADRPDVLDKLREENLRARNGDCNARLSMELFDKLPYTRAVVRETLRYRP 248939
248940 PVILVPYIAKKDFPITEKYTIAKGSMVLPSIWPATHDAEAYPNPDSFDPDRWITGTAEQH 249119
249120 PKNFLVFGTGPHYCLGQTYVQMNLMAIIGKASLALDWKHHITPDSEQIKVFATIFPQ (0) 249290
DDCLLTFRRQPGS*
62A Subfamily
CYP62A1 Aspergillus nidulans
GenEMBL U34740 L39121 (9392bp)
Brown,D.W., Yu,J.H., Kelkar,H.S., Fernandes,M., Nesbitt,T.C.,
Keller,N.P., Adams,T.H. and Leonard,T.J.
Twenty-five coregulated transcripts define a sterigmatocystin gene
cluster in Aspergillus nidulans
Proc. Natl. Acad. Sci. U.S.A. 93, 1418-1422 (1996)
gene stcB
CYP62A1 Aspergillus parasiticus aflatoxin pathway gene cluster
GenEMBL AY371490
complement(join(62552..62810,62866..64025,64093..64200))
gene="aflV
69% to CYP62A1 of Aspergillus nidulans (ortholog)
note the earlier 62A1 sequence is missing some internal amino acids
CYP62A1 Aspergillus oryzae
GenEMBL BAE71332.1, BAE59529.1, BAC20333.1
66% to CYP62A1 Aspergillus nidulans, ortholog
MTNTASRELIRAIEHVPLTWWFLAVGGAWIVSKIVKILQTAYFSPLRKVPGPWYARLTSARLAWASFANN
RIYYVQSLHEKYGPIVLIGPEEVDIADPVAAKQIHRMGSGFVKAPFYKLLSPGPVDNIFNFRDAKLHSTR
RKLYAKGFTLNSLRQQWEPTIRNIVALTVERIRHDAQQGEAEILGWWTLMANETVCKLTFNGGHDTVRNG
TKDPFVLMLERRMGDLAHLLQHFAPPLYYLGRLLGRAEPRLHDVFFSQETMFEAGKHVVAIARSARDAEG
DRNLFVKALAAGDLESKIGCLNDTEIITDAGALLLAGSDPTALSLTYLIWCVLNRPKLQADLESEVAGLQ
GDITDAACADLPTLNAVICESLRLYGPAPGSMPRSPPPDGATLCGYYIPPSAVVVTQNWSLHGNPKVWKD
PHTFDHTRWLPGSSLSEEAKMSFNPFGQGARQCLGIHLGWMQLRLATALFFRRCPGAKLAPSTTPESMVM
IDSFIAGMPKARRCAIQL
CYP62A1 Aspergillus flavus
99% to CYP62A1 Aspergillus oryzae
See fungal pages for sequence
CYP62A1 Aspergillus nomius
AY510454
complement(join(68188..68446,68501..69660,69727..69834))
gene CypX of aflatoxin biosynthesis
92% to CYP62A1 Aspergillus flavus
MINTPPRDLIRAIEQVPLTWWFLTVGGAWILSTIVK ILQTAYFS
PLRKVPGPWYARLTSARLAWASFANNRIYYVQSLHDKYGPIVLIGPEEVDIADPGAAK
QIHRMGSGFVKAPFYKLLSPGPVDNIFNFRDAKLHSNRRKLYAKGFTLNSLRQEWEPT
IRNIVALTVEKIRHDAQQGEAEILGWWTLMANETVCKLTFNGGHDTVRNGTKDPFVLM
LERRMGDLAHLLQHFTPPLYYLGRLLARGVPRLHDVFFSQEMMFEAGKSVVAIARGAR
GGEGDRNLFVKALAAGDFESKIGGLNDTEIITDAGALLLAGSDPTALSLTFLIWCVLN
RPKLQAELESEVAGLPEDITDAACAELPIMNAVIHESLRLYGPAPGSMPRSPPPDGAT
LCGYYIPPSAVVVTQNWSLHGNPKVWKDPHSFDHTRWLPGTSLSEEAKMSFNPFGQGA
RQCLGIHLGWMQLRLATALFFRRCPGAKLAPSTTPETMVMIDSFIAGMPKARRCAIQL*
CYP62A1 Aspergillus ochraceoroseus strain SRRC 1432
AY092402.3
join(18386..18418,18503..19650,19718..19979)
gene StcB/AflV in aflatoxin/sterigmatocystin biosynthesis
80% to CYP62A1 Aspergillus nidulans
N-terminal exon modified to be more like Podospora anserina EST CU876063
MDFFTSLGSTQWLSILVYVGGLWLLVKVFQVSL (0)
TLRIAYFSPLSRIPGPWYARLTGLRLSWSVFAN
NRIHYVHKLHQDYGPIVRIGPQEVDVADPALGREMHRMGSGFTKAPFYALLSPAPVDN
IFNFRDAKLHAARRKLYAREFTLQSLRKEWEPTIREITKMTVQRIKHDALNGEAEIMG
WWTLMANEVVCRLTFGGGAGTVAKGVKEPFVLMLERRMGDLAHLLKHFAPPGYYLGRM
LAHVIPQLHDVFYSQEKMFAAGGGVVSRARAVKRDGDDSGPSNLFNKALEAGSLTDTD
IITDAGALLLAGSDPTAISLTFLLWCVLSRPQIQHEVEAEVAQLEGEITDAACEGLPI
LNAVINESLRLYGAAPGCMPRSPPPGGATLGGYFLPADTVVVTQNWSLQRDPNIWENA
HSFDHTRLPREIRMTEQAKIAFNPFGHGARQCLGIHLGRVEMRLATAIFFRECAGARL
SESVTEESMQVVDSFIAGVPRDRRCTIRLG*
CYP62B1 Fusarium graminearum
FG05806.1 AACM01000233 FGcontig1.233_scaffold3
See fungal pages for sequence
CYP62B2 Nectria haematococca (Fusarium solani group)
Necha1/scaffold_23:519115-520695
66% to CYP62B1 Fusarium graminearum (probable ortholog)
See fungal pages for sequence
CYP62B3 Fusarium oxysporum
76% to CYP62B1 Fusarium graminearum
See fungal pages for sequence
CYP62B3 Fusarium verticillioides
90% to CYP62B3 Fusarium oxysporum = ortholog
See fungal pages for sequence
CYP62B Fusarium sporotrichioides
fragment BI188839 EST 88% to 62B1
PRVVPGKGATLAGHYLPAGTVVSTQAYTVHRDESIWPNALRFDGYRFMDKSRVTATQKSA
LSPFGAGSRV
CIGMHLAYMELRLGAALFFRECRGVKLGVEMTDEMMAMENHFLIAPKAHKCMVKL*
CYP62C1 Aspergillus nidulans
AN6414.1 49% to 62B1 53 clan
See fungal pages for sequence
CYP62C2 Aspergillus flavus NRRL3357
GenEMBL AAIH01000225.1
63% TO 62C1
1 AA diff to Aspergillus oryzae CYP62C2
MEWQIYLGIAFALWIVQ (0)
958 LYTAFTSPLRRVPGPLYTVLTRLPLKLASLTGNRIYFVHELHRKYGPIVRIAPDDVDISS 1137
1138 LAEFREIHRAGSPFLKSKWYEKFVPSKRSGVFTMRDPKEHAARRKLFARPFSKSELRRT 1314
1315 WEPAVREKVQLAVSQIQRELKAVGKSDLLKWWTFLATDVSGQLMFGESFNMLQLGK (0)1482
1535 KNQYINVLESTMMGSGIGAELPLVAWLGRHIPLSSFQNMFRATDYLFQYGQRAVTNSRTT 1714
1715 SNASRNIFAGMVYESEKGDGIITDEEVVLEAGNLIVAGSDTTAITLTYLIWAVLSQP 1885
1886 KLQRELEEEVNSLSADFDDSALEELPLLNAVIMEALRLYGAAPGALPRETPEGGAKFCGY 2065
2066 FIPQGMTVTTQAYSIHRDGDIYPDPERFDVSRWLKTETSASELAKKAFSPFGAGGRICLG 2245
2246 IHLAWMELRLATAQFFRECAGVRLAPSATWENMRPVNYFLISPRGHQCEVECNY* 2410
CYP62C2 Aspergillus oryzae
GenEMBL BAE55279.1, AP007151.1
475900 MEWQIYLGIAFALWIVQ (0) 475950
476022 LYTAFTSPLRRVPGPLYTVLTRLPLKLASLTGNRIYFVHELHRKYGPIVRIAPDEVDISSLAE
FREIHRAGSPFLKSKWYEKFVPSKRSGVFTMRDPKEHAARRKLFARPFSKSELRRTWEPAVREKVQLAVS
QIQRELKAVGKSDLLKWWTFLATDVSGQLMFGESFNMLQLGKKNQYINVLESTMMGSGIGAELPLVAWLG
RHIPLSSFQNMFRATDYLFQYGQRAVTNSRTTSNASRNIFAGMVYESEKGDGIITDEEVVLEAGNLIVAG
SDTTAITLTYLIWAVLSQPKLQRELEEEVNSLSADFDDSALEELPLLNAVIMEALRLYGAAPGALPRETP
EGGAKFCGYFIPQGMTVTTQAYSIHRDGDIYPDPERFDVSRWLKTETSASELAKKAFSPFGAGGRICLGI
HLAWMELRLATAQFFRECAGVRLAPSATWENMRPVNYFLISPRGHQCEVECNY
CYP62C3 Aspergillus niger
JGI gene model estExt_fgenesh1_pg.C_150110|Aspni1
67% to CYP62C2
See fungal pages for sequence
CYP62D1 Nectria haematococca (Fusarium solani group)
fgenesh1_pg.scaffold_70000012
Necha1/scaffold_70:35727-37407
43% to 62B1, 43% to 62C1, 37% to 62A1
See fungal pages for sequence
CYP62E1 Phaeosphaeria nodorum SN15
GENEMBL AAGI01000353.1
46% TO 62C1, 46% TO 62B1, 42% TO 62D1, 39% TO 62A1
MAFVDVVTANKPLLVALI (0)
FVYVGLTCPTRHLPGPWYTRFTHYRLKRAVVTGQRIFYIDALHKQYGPIVRLSPTEV 956
GVADLDAFKEIHKIGTKYMKSEWYLRLANFPKAGVFTMLDPREHGARRKLLSRSFSRSY 1133
LVENWEPAVREKALLVVTKIKVDAMQSTADVYNWWMLLASDVSAHLAFGESFRMLETGH (0) 1307
ANQFIRVLKKLTMGAGIMVEMPFLRLLRFVPINAVQEMFNANEFILTGAGRAVEMARSRT 1542
GESNIFAKVIEDCEKEGEGHIDDMDVRIEAMNIIIAGTDTTGVTLTYLTWAVLQQPQ 1713
LQSALEVETAGLRENFTESDLINLPLLNATIEETLRLYGAAPSSLPRVVPQGGTRFSGHY 1893
IPQGVTVDTQAYTFHRDPRIWSDPLTFDPQRWISSQEGYTAEALSPDAKTAFHPFGVGAR 2073
SCIGIHLARMELRYAVAFFFRECRGIRLAPSTTLESMEFENFFLIAPKAHRCDITFQQ*
CYP62F1 Mycosphaerella fijiensis
JGI gene model e_gw1.7.207.1 revised at N-term
40% to CYP62B2 Nectria haematococca
41% to CYP62B1 Fusarium graminearum
See fungal pages for sequence
63A Subfamily
CYP63A1 Phanerochaete chrysosporium (white rot fungus)
GenEMBL AF005475 (208 amino acids)
Kullman,S.W. and Matsumura,F.
Identification of a novel cytochrome P-450 gene from the white rot
fungus Phanerochaete chrysosporium
Appl. Environ. Microbiol. 63, 2741-2746 (1997)
CYP63A1 Phanerochaete chrysosporium (white rot fungus)
No accession number
Jagjit Yadav
Submitted to nomenclature committee Dec. 15, 2000
Clone name Pc-1
Aside from intron editing, this sequence and AF005475 have only
10 aa differences clustered in one region and are probably
the same gene
JGI gene model ug.20.36.1
MGLTQAQRLVLGQLARLVAPALAVCVLLAAARRTQLVRAPVWADALIALIAIPLFHVGRA
HWRYARLARKAARLGAALPPRWEGKLPGSVDVLQLVDEAYRRGFLSDYFYEKFGELGHTY
NFYVLWDMDYCTEDAAVIKAVLATDFNNWVKGERFDSYMHSVLGTGVFNADGELWKFHRS
MTRPFFARERITDFETFNRHAEEAILKMKERLREGFAVDFADLISRFTLDAATEFLFGAC
VHSLAGALPYPHGAPAHLHTTRARIPADDFAAAFRAAQDAVSHRARLVWLWPWFELARSR
TDTPMRTVDRYLTPIIERALAMSRAAKQAPQGEKEEVADGETLLDHLARYTTDPTILHDE
ILNIMIAGRDTTGGTLTFVIYFLTQHPDVLQRLRQEILDVVGPSNLPTYDDIKQMKYLRA
VLNETQRLYPPVPWNMRYAVEDSIVPNSEPEGKPWFIPAGASVSYSVHCMHRRKDYWGPD
AEEFDPDRFLDERLHKYLTPNPFIFLPFNAGPRICLGQQFAYNEMSFFLVKLLQTFEDIS
FERDAFEPNALPPAEWAKFPGRKGKEKFWPRAHLTLYSEGGMWVKMREAQAMGQVA
CYP63A2 Phanerochaete chrysosporium (white rot fungus)
No accession number
Jagjit Yadav
Submitted to nomenclature committee Dec. 15, 2000
Clone name Pc-2 JGI gene model ug.20.35.1
85% to 63A3, 59% identical to 63A1
MLVSVDALALRTLVYELTYLLYPAVPTAAALILLQRFGNVWLPTWTIVLLSLCNVPVAHR
ILVWLKDGRAARKAASMGAILPPRLKGRWPGSIDLLRQLTQTFETGFLSEMLWGYMHVLG
QTFEVYILWDSNYVTSDANVIKTILATDFDNFVKGEKLDVCVRPVLGTGVFNSDGEMWKF
HRSMTRPFFTRERISHFDLFDRHADATMAKMKARLAEGFAVDFQDLISRFTLDSATEFLF
GQCVHSLASVLPYPHDAPAHLQTTGASRTEDFARAFAEAQDAVSFRLRMGWLWPWFELFG
SRTKAPMAVVDAFLDPILRDAVARADKIKRENGGRVPEVKGEIEEDETLLDHLVNVVQTK
ILHDEVLNIMIAGRDTTGGTLTSAVYFLSQYPEVLRRLREEILEKVGPTRRPTYDDIREM
KYLRAFINETLRLYPAVPWNVRYPVKDTTIPGPHPDKPYFIPANTPVSYSVHCMHRRTDY
WGPDAEAFDPDRFLDARVQRYLTPNPFIFLPFNAGPRICLGQQFAYNEMSFFVIRLLQHF
DEVQLCEDALAPDCRVPDAWRGAPGRKGVERFWPKAHLTLYAKGGLWVKMREASTSEAVV
CYP63A3 Phanerochaete chrysosporium (white rot fungus)
JGI geme model ug.20.34.1
MPSSIDFPDRLVLRVIAYELVFLFYPAVPAAAGLVLLRRLTDIWLPTWAIVLLSVCSLPVVHGLSIWR 26138
26137 NHWRAARKAARMGAVLPPRLKGRWPGSIDLLMRLTDAFETGFMS (1) 26006
25949 DLLWEYMHTIGQTFEVYVLWDSNYVTSDANVVK (0) 25851
25697 AILATDFTSFVK (1) 25662
25698 GKKFDVCMRSVLGTGVFNSD (1) 25639
GDMWK (2)
FHRTMTRPFFTRERISHFDLFDRHA (1) 25433
25379 DDAMAKMKARFAEGYAVDFQ
DLISRFTLDSATEFLFGQCVHSLASVLPYPHNAPAHLQ 25206
25205 TTSASAAEDFARAFAEAQTVLNFRIRMGWLWPWFELFGSRTKAPMAVVDAFLDPILKAAV 25026
25025 ERADQIKHENGGKVPEAKEEIDEDETLLDHLVKYTN (1) 24918
24855 DPKILHDEVLNIMIAGRDT (0) 24805
24748 TAGTLTSAVYFLSQYPEVLRRLREEILEKVGPTRRPTYDDIREMKYLRAFIN (1)
ETLRLYPAV (2) 24515
PWNVR (2)
24380 YPVKDTTIPGPEPDKPYFIPANTP (2) 24318
24258 VSYSVHCMHRRTDYWGPD
AEAFDPDRFLDARVQRYLTPNPFIFLPFNAGPRICLGQ 24091
24090 QFAYNEMSFFVIRLLQHFDEVQLCEDALAPDCRVPDAWRGAPGRKGVERFWAKAHLTLYAK (0) 23908
GGLWVKMREAPTSEAV* 23800
CYP63A4 Phanerochaete chrysosporium (white rot fungus)
JGI geme model pc.151.16.1
PC-4
MALPPGLQYLLPQLPLLLAPPAAVLLAAHAARAFAGTAAPAWALALACVLSWPVALTALV
QLRAHRVAREAAARGARLPPAVEARYPGGVDLMRRNNSEVEEHIPGYRLSEFGRQYGWTY
NFRMLFQDRVRGRPRPRPPGRILATDFTSYEKGAVFSAQMKSLLGTGVFNADGDLWKFHR
AMTRPFFSRDRISHFDVFDRHAEDALKLAKARLSEGVPIDWQDLVSRFTLDSATEFLFGQ
DVRSLSAPLPHPPTAPQAQHDTHDAEHPANRFAHAFLQAQLASARRSRYTAAWPLWEFWE
NKVEKHTRVMDEFIQPLLRDALARKAKGADAQAEEAVADGETLLEHLVKLTDDPQIIHDE
TLNILLAGRDTTAITLTMAGYMLAEHPDILQRLRKEILDTVGTRRPTYDDIRDMKYLRAF
INEVLRMYPPVPFNVRFSTAPTVWPSPEGDFYVPAGTRCMYSVFVMHRRKDLWGPDADKF
DPDRFLDERLGKYLTPNPFIFLPFNAGPRICLGQQFAYNETSFMLIRLLQRVSKIELHPE
VSPQSVAPPGWAASSISDGKDKVVFKSHLTMYVQGGLWVTMQFENPEEH
CYP63B1 Phanerochaete chrysosporium (white rot fungus)
JGI geme models genscan.57.18.1 and genewise.57.16.1
PC-7
MPHPFSRYRLRVFGDFVRIVLAPSFVFWSAVQILKLRLGLLSPAAWLTFLFAASYARVQY
RGFLQRQEARRRGGVLPPEVVGRWPGNIDILIKLGKASLTAYPGSFYLDLFEEYQSTTLN
LKLLWSDLVRCLSFCRLSAVLKTLSQIITMDEEHIKHILTTGFNHFWRGRRQKERMYAPS
GASRRHDTDSQGDVSQEWKKHRALARPFFARDRISDFDLFEKYAGATLGILGGLAGRGAA
VDVQDLYARFTLDAAAEFLFGERLDTLHGALPVAGQAKLGSKGAATDDAFGAFVRAFEAS
QDIITTRQVRGYFWPVRELFQDKVAPHAAVIGAFLEPIVQRTLDRKAKMRAAGVSPTTEH
DTFLDYLADHTEDPKVIRDQLLNILMAGRDTTACLLTYVTYVMAMYPDIMQKMRQEVLHV
CGHDAPNFEKLKALRYVHAVLNETLRVFPPVPMNVREVRARGVVLPHADPTYAAAPAPLY
VPGGTVVMYLPVLTQRNTALWGDDADVFDPDRWLDARLRRFTENPMMYTPFSGGPRICIG
QNYARNEATYLLVRLLQQFDAVALAPEAQPAGSLPPPEWRHARGRAAEERIWPAYAITLY
VKVRLSLQWLYC
CYP63C1 Phanerochaete chrysosporium (white rot fungus)
JGI geme model pc.101.32.1
PC-5
MELHPRQYRLRFLLDVLRAIVWPQLVFNAALYLAGFHPGAFLRVVASVLAVPLLGTVRTA
ISQRRNKIQAGAALGAKEVPCVRGKWPGNLDIVLGFVRSLKEAYLMQFLDDLFREYDCKT
LNMRLLWEDQIWTIDEAHVRYMLAGPGFEWFHKGYYWQERMESFLGNGIFNRWAQRAIAR
PWFVKDRISDLNIFDRHTTTTLALISEFVDRREAFDAQDLFARFTLDSASEFLFGRCLDT
LHGTLPVAGRAKMGPKGTAIEDAFGSFARAFEDVQVQIARRTRIGKPWPLFELFTDKTAP
SVAVIHDWLRPIVHEALAKKSAASAEKESGEDSTFLSHLANSTDDPQDIAYSVLNMLLAG
RDTTASVLSFVVYFLALHPHVTEKLRAEILQAYGPDGRPSVEDMKDLKYVRAVLNETMRL
FPPVPMNLRLSDAHPRIFPASGSAPKYYVAPRTVILYSIFLVQRRTDLWGADALEFRPER
WLEPATARLLADHPFAFTPFHAGPRLCLGQNFAYNEMTFFIVRLLQRVSGFELAPDAQPE
GSLPPARWKYGEGRQAVEKIWPASSVTTFIKVSLASMPCCGERWLKRRRQGGLWVRAVPA
CYP63C2 Phanerochaete chrysosporium (white rot fungus)
JGI geme model pc.101.28.1
PC-6, partial only 387 amino acids, needs more work
QRAIARPWFAKDRISDLNIFDRHTSTTLALIADFADRREAFDAQDLFARFTLDSASEFLF
GKCAETLHGTLPVAGRAKLGPKGSSVEDEFGSFAWAFEELFHDKTAKHRKVIQDWLQPIV
REALHSKAAAARGEDTGEGTFLSHLTKTTDDPQDIAYSILNMLLAGRDTTAAALSFTVYL
LALHPEVVEKLRAEVVQAYGSDGRPSVEDMKSLKYLRAVLNETMRLFPPVPLNIRTSDDT
PRVFPASAGAPKYYVPPRTPVVYSSVIIQRRKDLWGADALDFRPERWLEPETARRLAENP
FMFMPFHAGPRLCLGQNFAYNEMSFFVVRLLQRVAALELAPDAQPEGSLPPARWKNGEGR
QAVEKIWPGSSVTTYIKVSSTRSRPCG
64A Subfamily
CYP64A1 Aspergillus flavus
GenEMBL U81806(2760bp)
Prieto,R. and Woloshuk, C.P.
ord1, an oxidoreductase gene involved in the conversion of
O-methylsterigmatocystin to aflatoxin in Aspergillus flavus
unpublished
CYP64A1 Aspergillus parasiticus
GenEMBL AF017151
Yu,J., Chang,P.K., Ehrlich,K.C., Cary,J.W., Montalbano,B.,
Dyer,J.M., Bhatnagar,D. and Cleveland,T.E.
Characterization of the critical amino acids of an Aspergillus
parasiticus cytochrome P-450 monooxygenase encoded by ordA that is
involved in the biosynthesis of aflatoxins B1, G1, B2, and G2
Appl. Environ. Microbiol. 64 (12), 4834-4841 (1998)
97% identical to Aspergillus flavus CYP64A1 (0rtholog)
CYP64A1 Aspergillus parasiticus aflatoxin pathway gene cluster
GenEMBL AY371490
join(57543..57738,57815..57944,58000..58191,58256..58407,
58457..58701,58759..59030,59089..59488)
gene="aflQ"
function="conversion of O-methylsterigmatocystin to
aflatoxin B1 and aflatoxin G1 and
dihydro-O-methylsterigmatocystin to aflatoxin B2 and
aflatoxin G2"
CYP64A1 Aspergillus oryzae
GenEMBL BAE71330.1, BAE59527.1
99% to CYP64A1 Aspergillus flavus
MIYSIIICAGALLGLWILEKLLAPKDTRPPLPPGPWRKPIIGNLTDFPPKGTPEWLFWAKHQERYGPMSS
LEVMGQTIIMINDAQLGIEIMHKKSALSQMIPDAPFAHMAGWGMSLATERNRQAWKTIRANMKQEIGTRR
AISTFHSKMEIGIRRFLLRTLDNPDDLRFHIRKEANAFMMDVAYGYTIAPHGKDELYDLTQQSVRQFSHI
FSPGEWSVNFFPILRYVPSWFPGASFQIKAAEYKWTIERMTMVPYLWIKDQVARGCSRPSILLRLLQKGH
YESGSHQEQVLVWTNAEFVMGGSDTTVSAVSSLFVAMALYPEVQRKAREELDRVVGPTTLATFEHRSQLP
FIDALVKEVFRWHPASPLGAPHITQEDQIWDGYLLPKGALLLPNIWTFTHDPSVYHDPMVFKPERFLEGK
DSPPETDPMKFVFGFGRRICPGRFVTDEKLFLIACHAVSCFFISPKDPGAPEPDWLPGVISQPGTFDLNV
VPRSPAHEELIRSIETDHPWKNADATDISRFMARNQMI
CYP64A1 Aspergillus nomius
AY510454
join(63157..63352,63429..63558,63614..63805,63870..64021,
64071..64315,64373..64644,64705..65104)
gene OrdA of aflatoxin biosynthesis
93% to CYP64A1 Aspergillus flavus
MIIGLIICAGALLGLWLLEKLLAPKDTHPPLPPGPWRKPIIGNL
TDFPPKGTPEWLFWAKHQGRYGPISSLEVMGQTIIMINDAQLGIEIMHKKSALSQMIP
DAPFAHMAGWGMSLATERNKQAWKTIRANMKQEIGTRRAISTFHLKMEIGIRRFLLRT
LDSPDDLRFNIRKEANAFMMDVAYGYTIAPHGKDELYDLTQRSVRQFSHIFSPGEWSV
NFFPILRYVPSWFPGASFQVKAAEYKRTIEKMTMVPYLWIKDQVARDCCRPSVLLRLL
QKGHYESGSQEEQVLVWTNAEFVMGGSDTTVSAVSSFFVAMALYPEVQRKAREELDRV
VGPTTLATFEHRSQLPFIDALVKEAFRWHPASPLGAPHITQEDQIWDGYLLPKGALLL
PNIWAFTHDPSVYQDPMVFKPERFLEGNGSPPETDPMKFVFGFGRRICPGRFLTDEKL
FLLACHTLSCFLVSPKDPAAPEPDWLPGVISQPSPFGLNVVPRSPAHEALIRSIETDH
PWKNADATDISRFMARNEMI*
65A Subfamily
CYP65A1 Fusarium sporotrichioides
GenEMBL AF011355
Alexander, N., Hohn, T.M., and McCormick, S.P. (1998)
The TRI11 gene of Fusarium sporotrichioides encodes a cytochrome
P450 monooxygenase required for C-15 hydroxylation in trichothecene
biosynthesis.
Appl.Environ.Microbiol. 64(1), In Press
The TRI11 gene catalyzes the hydroxylation of isotrichodermin to 15-
decalonectrin
submitted to nomenclature committee
CYP65A2 Fusarium graminearum
FG03540.1 AACM01000159 FGcontig1.159_scaffold2
See fungal pages for sequence
CYP65B1 Neurospora crassa
AABX01000364.1 cont3.489
Neurospora crassa sequence contig 1.742 (supercontig 102)
42% to CYP65A1
MNLSHLLPLGIPLPSPTSLIGIFLIG (0)
TSILVSSILLSAIYNLTLHPLARHPGPFFHRASILPYLYRQITGTLPCSILDFHARYGPVVRISPNALSFADPQAWKDIY 26703
26702 GHRPHGEEEFAKLNLFYRIKGSPPSLLSETKEAHGTLRKLMAHGFSDRSMRAQEGIIGGYVK (1)
EEQDERVPLDMVSWYNWTTFDIIGDLAFGEPFGCLEKAEYDPWVDAVGKSVRFGCVMFA
LRLLGLEDWVCPLVRKLSGNARRFHRKRTMDKLQRRVKLTKERPDFLEGLLQKREEW (0)
GIDMDALAANASLLIVAGSETTATLLAGATYMLLRDPEAMKKLTEEVRSTFKSEEEITL 25711
25710 SSVGNLEYMLACLNEAMRLYPPVPIGMPRVVPKGGAKVAGTFVPEG (0)
TVVAVWHWATSHNEQHFVEPFEFHPERWMQDPRFANDRLD 25372
25371 AVQPFSVGPRNCIGR (2)
SLAIAEMRLILTKVVYNFDMKLANPEKDWLDQHIYTLWDKHPLPVYLTPIERNGV*
CYP65B1 Neurospora discreta
JGI gene model scaffold_9-snap.228.1
85% to CYP65B1 N. crassa
gene model does not appear to be assembled
correctly. One low complexity sequence
removed. mRNA may be needed to decide this assembly.
See fungal pages for seq
CYP65B2 Magnaporthe grisea
MG06544.4 55% to 65B1 new N-term AACU01000188 cont2.1218
See fungal pages for seq
CYP65C1 Neurospora crassa
AABX01000034.1 cont3.25
Neurospora crassa sequence contig 1.96 (supercontig 12)
45% to 65B1
MPVIDKLAGVTGLSGLHLLLGAVGVT (0)
ILLYIIGSAIYNVFFHPLASYPGPLLQRASPIPFSIRHALGVQPFHTQALHDKYG
PVVRIAPNHLSFTDVRAWRDIFGHIAGSRHGQEELAKFKS
FVRPIDAQPIHIISAGREKHGQLRRALANSFSDASMRGQGPLIGKYIDLLLHKLH
EQGQEGKVPLNATSWYNSVTFDVAGDLIFGMSFGALDQNGNHSWLQYILGSLSSLAPMAA
LSYAGLHWLVQLLWRAKGAEVFRKSMESVDLMLKERLKMPVDRNDLFEGLVQRQEKL (0)
GLSFEELASNAWLLVL (2)
AGADTTATTLSGTTYLLTQHPEVLKRVTQEVRSSFKSVEEIDISSVNRLTYMLAVLNEA 64919
64918 MRLYPPVTSLTARIVPRGGAHVLGEYLPEG (0)
64756 TIVDIQQWSMNHSKENWVDPWAFNPARFLDDKETAREKGNVLDALQTFSVGPRNCIGR (2) 64580
NLAYAEMRWILARILFDFDLRGAPTNQNWIERQKAYGLWDRIPLDVYFEPARERK*
CYP65C1 Neurospora discreta
JGI gene model estExt_Genewise1.C_41538,
gene model incorrect. Added I-helix
87% to CYP65C1 N. crassa
see fungal pages for seq
CYP65D1 Magnaporthe grisea
MG03832.4
47% to 65B1 partial seq. length 53916
runs off end, changed C-term AACU01001030.1 cont2.747
N-term on AACU01001031.1 cont2.748
seems to be missing some N-terminal seq.
see fungal pages for seq
CYP65E1 Magnaporthe grisea
MG03830.4 37% to 60A3 AACU01001030 cont2.747
see fungal pages for seq
CYP65F1 Magnaporthe grisea
MG08498.4 42% to 65A1 AACU01001266 cont2.1600
see fungal pages for seq
CYP65G1 Magnaporthe grisea
MG00023.4 39% to CYP65A1 41% to 65B1 AACU01001352 cont2.7
see fungal pages for seq
CYP65H1 Magnaporthe grisea
MG10954.4 39% to 65B1 40% to 65A1 41% to MG05390.4
AACU01001824.1 cont2.2124
see fungal pages for seq
CYP65J1 Magnaporthe grisea
MG10070.4 39% to 65B1 , 40% to 60A2 AACU01001328 cont2.1928
see fungal pages for seq
CYP65J2 Alternaria solani
Alt3 BAD83682 69% to 65J1 Mg
PKS for alternapyrone is next to three P450s called alt1, 2, 3
Chem Biol. 2005 Dec;12(12):1301-9.
1 MALSSALDSL WHQLDQLLSL INRNIITGII VLPVLYVLLK VIYNLYLSPL AGYPGPKLWA
61 VSRLPWNRAN MKGRISWKIR ELHDKYGPVV RIAPDELSYT TSGAWKKIYG QRNPEFVKAL
121 DGRGIAPASI GGQRSLMTEH QDKHLRLRRA IDPAFSQRAL REQESYFQDH SDNLVQKLKQ
181 RCKDGPLDMT TWYNLVAFDI VSDLAFGEPS GCVNNPDQPW IQAILARAKA IVWFQLAVQY
241 GFMGLLNWMT PKYVTESRKK HIAMTEAKLK ARVEAKNPGK DFMSYILEND EKLNHLELVM
301 LSSNFIVAGS GTSAGGMSGL TYLLLRNPDK LEKLKQEIRG LFKSRADMTL QAVTSCKYLR
361 ACLNEGMRLY PPTPGSLPRF VPGKGEMIEG KWVPGGYAVG VNQLAAGHSE RNFKKAREFH
421 PERWLDEPDS EFKDDDRSAV QPFSYGQRGC IGRSMAYAEM SLTMAKLVWY FDWELDEPDN
481 DWWNQQGTYL VWEKLPLQVK LTPVSDVVE
CYP65K1 Magnaporthe grisea
MG09945.4 poor match 41% to MG02982.4
AACU01001747.1 cont2.1897
see fungal pages for seq
CYP65L1 Magnaporthe grisea
MG02982.4 42% to CYP65B1 38% to 65A1 AACU01001589 cont2.600
see fungal pages for seq
CYP65M1 Magnaporthe grisea
MG00651.4 37% to 65B1 AACU01001445 cont2.120
see fungal pages for seq
CYP65N1X Magnaporthe grisea
Name changed to 563B1
see fungal pages for seq
CYP65P1 Magnaporthe grisea
MG07983.4 48% to 65B1 partial RUNS OFF END
AACU01000985 cont2.1482 C-TERM HALF
see fungal pages for seq
CYP65Q1 Fusarium graminearum
FG07765.1 AACM01000318 FGcontig1.318_scaffold4
see fungal pages for seq
CYP65R1 Fusarium graminearum
FG03191.1 AACM01000148 FGcontig1.148_scaffold2
see fungal pages for seq
CYP65R2 Fusarium oxysporum
82% to CYP65R1
frameshift at TLQ/KMH
see fungal pages for seq
CYP65R2 Fusarium verticillioides
95% to CYP65R2 Fusarium oxysporum = ortholog
see fungal pages for seq
CYP65S1 Fusarium graminearum= Gibberella zeae
GenEMBL AACM01000323
complement(join(168239..168404,168457..168623,
168682..168761,168816..169001,169050..169836,
169886..169960))
locus_tag="FG08023.1"
sequence revised added back GMSRPEIIENSSLLIV to gene model
MAIISTDLASGTSLGGGLVLLAAAVVLFVTGRAIYYVFFHPLAK
IPGPKLYAATQLPYLYHLTRGQWVYRLKELHEQYGPVVRYTYNDVSFITADAYKTIYG
HKTGGAREFGKDFRLYRQGRPAPSLIVTKHEDHKRQRKLLSHAFSHKALSEQNDLLMH
YVNLFIEKLTQKAREGESVDMVAWYNFATFDLIGHLALGQPFGCLESGKYHPWVTKIF
SSIKGISFSQAAIRLGLHNWISLITPAHVKKATKEHFEFTEHVAGARLDVKDNNSKDFMSYILRYNDER
GMSRPEIIENSSLLIV
AGSETTATLLSGVTYQLLTNPEAYKKLVTEIRLAFATEEDITAAAVD
KQTYLLAVLSEGFRMYPPVPGALGRTIPKGGDFVEGHWFPENVGVSVPQLPAYHSARN
FKDPEKFVPERWMDDPRYSNDCRAVVQPFSMGPRDCIGKNLAYLEMRLLLTRLLWKFD
AELMPESREWKEQKIFTLWEKGSLHVKLTEVVREKN
CYP65S2 Nectria haematococca (Fusarium solani group)
JGI gene model e_gw1.7.949.1 short on N-term
Necha1/scaffold_7:62397-63948
54% to CYP65S1 Fusarium graminearum
see fungal pages for seq
CYP65S3 Nectria haematococca (Fusarium solani group)
JGI gene model fgenesh1_pg.scaffold_4000060
Necha1/scaffold_4:143263-145075
56% to CYP65S1 Fusarium graminearum
60% to 65S2
see fungal pages for seq
CYP65S4 Fusarium oxysporum
58% to CYP65S1 Fusarium graminearum
see fungal pages for seq
CYP65S4 Fusarium verticillioides
94% to CYP65S4 Fusarium oxysporum
see fungal pages for seq
CYP65T1 Aspergillus nidulans
AN7522.1 38% to 65A1 53 clan
see fungal pages for seq
CYP65T2 Neosartorya fischeri NRRL 181
AAKE02000011.1
DS027697.1 complement(627606..628748)
57% to 65T1
MMFVILAAASLLYISYILLLAIKTIYFHPLSHIPGPKWRIGIPLLHHLS
628603 AIRGRLDLDISSWHEKYGDVVRSGPDEVTFITAQAWKDIYGHGHRQLPKVQISAINGKDI 628424
628423 FSANDVDHARFRKALSHAFSAKGLQAQERIVTQYIDKLIQRLKWFAESGTAADMGKW 628253
YNLATFDLIGDLAFGEPFGGLDSA
EYHHWVATMFGFVKSIPFLRALHRYPVVFKVILAFHPGSLMEMRSKQVEHAKATVQKRL
SNSAAGRGDFMDSMLRHQGDKGGLSLDELEENANVLILAGSETTAALLSG
VTYWLLRTPDALDKVMREVRAAFALERDITFNQVTAKLPYMLACLNEAFRLYPPVPGGLQ
RWTEVPTWISGYRVPAN (0)
TKVSVHQLSAYSSLKNFHQADRFIPERWLPEAIEDPASPFFSDNRAVFQPFSIGPRNCLG
RNLAYTEMRVILARVLWTFDLTLCEESRDWKDQKIFVIWERGPLMCKLAMRDDRGDCQGGR*
CYP65T2P Aspergillus fumigatus Af293
XP_748490.1 also AAHF01000010.1
58% to 65T1 Nterm aa 52-168 plus C-term part, 84% to 65T2
name revised from 65T3P
SLRGRLDLDLRSWHEKYDGVVRPGPDEVTFITAQAWKDIYGHGHLQLPKVQISTINGK
NIFATNDVDHARFRKALSHAFSAKGLQAQECLVTRYIDKRIERLKGFTESGTAADMGKW 425909
*NLATFDLIGDVAFGEPFGGLD
AYSSPRNFHQADRSIPERWLPQVIENPASPFFLDNRAVFQPFSIRPRNCLGRNLAYTEMR
MILARGLWTFXX
LCEESRDWTDR
CYP65T4 Aspergillus oryzae
GenEMBL BAE59716.1
68% to 65T1, 45% TO CYP65AF1
MPFNILLWPVIAFFLYSLLTAIWYIYFHPLRRIPGPKSWIIFPIMRHASAIRGRFDIDMRQHHAVYGPVV
RFGRDEVSFITAEAWKDIYGHGHQQLPKVLSSASNMLDIISSNDTDHSRYRKALSHAFSAKGLQAQEPLL
NSYVDKLIERLKGIAESNLPADMVKWYNLTTFDIIGDLAFGEPFGGLDNSEYHHWVSTIFESIKAIPFLK
LKDAYPLAFKAILGLIPKGIMEARKRQLEHSRITVQKRLQTSSSYNRGDFMDSMLRNRGEKDSLNDSELE
ANSNILIIAGSETTATLLSGATYWILRNPEALAKLTDEVRSVMKSEPEITAQKASAELPYMLACFDEAFR
LYPPVPTGLQRRTLVPTRISGCDIPAGTKVSVHQSAAYWSSTNFHAPDRFIPERWLPEAKSDPSSPFYSD
NRGVVQPFSTGPRNCIGRNLAYAEMRVILARVLWNFDLELCEESTQWSDQKAYTLWEKPPLMCRLKLRES
FRG
CYP65T4 Aspergillus flavus
99% to CYP65T4 Aspergillus oryzae
see fungal pages for seq
CYP65T5 Aspergillus terreus NIH2624
NW_001471196
68% to 65T4, 62% to CYP65T1
MALQEFLPVVPAISVLYIFLLAIWNLYFH
PLRHIPGPRSWIAFPIMRHISASRGRLDSDMRRFHAQYGGAVRLAPAEVSFITPDAWKTI
YGHGHTQLPKVQTSESKGLDIISSEGPNHTRHRKALAHAFSARGLQAQEPLVRGYVDKLI
ERLKEFAESQLQVDMVKWYNLTTFDLIGDLAFGESFGGLDNSRVHSWVSTIFRSVKVLPF
VRITDTYPILIPLLMALLPKSLRTARRDQTNYSKETVHKRLANTAAHGRGDFMDSMLRHR
GEKDGLSDRELEENASILIIAGSETTATLLSGVTYWLLRSPEALAKVTDEVRSTFQTEGE
ITLQDVGARLPYMLACLDEAFRMYPPVPCALERRVLTPIVIAGYNIPPG (0)
TVVSVHQSAAYCSPANFHRPQDYIPERWLPDAKSNPSSPYFSDQRDVLQPFSVGPRNCIG
KNLAYAEMRLILARVLWNFDLELCEESLHWKDQKSYLLWDKPPLMCKLKQRI*
CYP65T6 Aspergillus niger
JGI gene model e_gw1.4.330.1|Aspni1
65% to CYP65T4
see fungal pages for seq
CYP65T7 Aspergillus niger
JGI gene model fgenesh1_pg.C_scaffold_3000290|Aspni1
68% to CYP65T4
see fungal pages for seq
CYP65T8 Aspergillus clavatus
69% to CYP65T2 Neosartorya fischeri
see fungal pages for seq
CYP65 fragment Aspergillus ochraceus
DQ054597 C-term piece after heme signature
Involved in ochratoxin synthesis
See DQ054596 for a second P450 in this pathway
This fragment is 60% identical to CYP65T5 C-term
FLRAQGTASERNLAYAEMRLIAVKLLWNFDLAFEEECEGWDNQK
SYNIWEKDPLKVKLTP
CYP65U1 Aspergillus nidulans
AN6466.2 37% to 65B1 53 clan
revised 7/18/07
MGVYLFSSSLWATLLLAILIYRLVRITYNLYFHPLSGIPGPSAWAASRLP
FVYALIRGTIIQDFQKLHEQYGPIIRIAPNEVTFAHPEAYTDIFQPRPEK
SQGQFLKDPLWWARQPGHPDSLLSVINPERHAQMRRVLSPGFTARALRKQ
EPFVQKYVNLLVAQLQDLVSKSPSAHINMTPWFNYTTFDIFGDLGFGESF
DCLQHSRYHPWIALLFDSVKAAGFVIATRYYPLIEAVLLKCVPPSMRKIQ
RDHYQQIVDKVQRRLGWELQRPDFMSYVIDERGGLRLDAGELYATFMILT
TAGSETTATALTGTLNYLVNHSPESLQRLENEIRGAFSGLDAITLDAVRN
LPFLNAVIQEGLRLCPPIPWVLPRLVPQGGSMICGTWLPGGTPVSIQAYT
LNRDPTLFNRATSFLPERWLASSTSDSSSCFFNDQRQVVQPFSTGPRACL
GQHLAWAEMRLILAKLVWVFDFGAIDGQGVNWEDLRTYLLVERKPIN
VRISSRTV*
CYP65U2 Aspergillus niger
JGI gene model e_gw1.3.403.1|Aspni1
61% to CYP65U1
see fungal pages for seq
CYP65V1 Nectria haematococca (Fusarium solani group)
JGI gene model gw1.24.106.1 model short on N-term
Necha1/scaffold_24:57028-58643
49% to CYP65B1 N. crassa
see fungal pages for seq
CYP65V2 Aspergillus oryzae
GenEMBL BAE61720.1, AP007163.1
53% to 65V1, 43% to 65B1
revised 3/20/2009 with a frameshift = &
MMLNSINIPQNAISWAVACAAA (0)
198235 ATFCLASSVVYNIFFHPLARYPGPLSHAASRVPYFIRVFKGTLPFD
MLDFHTRYGDVVRIAPDELAFSHPDAWKDIMGHSKGGLYLEKASWFYRP
LEYDPPHIFNEGRQAHSLLRRQLAHSFSDKGMRDQEPMIRGYVDLLLQRLREMGGREDTVDISAWYNYTT
FDIIGDLSFGESFGCLKGSNYDEWIESIFRAFPFITALQALSFTPLVKEVLLALVPPSMRDRRERLRDFT
RQKMLRRMAIAEERPDLIEGLLKKKEEL (0)
GLTIDKLTA &
AETLVVAGSETTATLLSGVTYLLLKNPEAYQ 199191
RLREEVRSTFSSEDDINLSSAAKLPYMLACVEEALRMYPPGA
IGPPRVTPKGGAEILGARIPGRTYVSIHHWALYRREEYFTDPHTFHPERFLGDPRFSNDRHNVLQPFHIG
PRSCLGRNLAYSEMRLILALLFFNFDMELSDDSQDWIQQKNVIMWQRGPLKVHLTYIHRNSA
CYP65V2 Aspergillus flavus
99% to CYP65V2 Aspergillus oryzae
see fungal pages for seq
CYP65V3P Aspergillus oryzae
GenEMBL BAE55600.1, AP007151.1
Gene model has wrong C-term, revised
about 60% to CYP65V2, 48% to 65B2, 54% to 65V1
one stop codon and one frameshift in last exon
MSTAALVNAKSVLIATISA (0)
PIIYMLGMAIYNVYFHPLNRFPGPVSHAISRIPYFYRAVRGTLPFD
MLKLHERYGDIVRIAPDELAFSHPDAWKDIFGHKNGEPEMAKAAWFYRPLDDPLHIVNEDTDEHRRLRRQMAHGF
SEKSMRAQEPIIRKYVDLSLEKLHQSCKNGSLVILDWYNFTTFDIIGDLAFGEPFGCLEGCNYDQWIKGI
FKGAYLGSFMQALSFVPRLKSTLLLLVPKSMQEAHQGHKALTKAKMLRRAAITEKRPDLIDGLLRNKDEL
1455183 KLGLDKLIANAEILIIGGSETTASLLSGVTYLLLQNPNAYETLKNEVRSKFNHQEEIN 1455356
LISVNKLSYILA
CLDEALRMYPPIANGLPRVCPKEGSWVLGEYIPGKTVLSIHQWALYRREKHFKDPNTYHPERFLSSPEFL
DDRRDAFQPFHTGPRNCLGRN (2)
LAYS*MRLILALVIFNFDMRLPQRAKDWIRQRNYLMWDKGPLKVYLRPRTSI*1455965
CYP65V3P Aspergillus flavus
98% to CYP65V3P
see fungal pages for seq
CYP65V4 Aspergillus niger
JGI gene model e_gw1.11.216.1|Aspni1
70% to CYP65V3P
see fungal pages for seq
CYP65V5 Histoplasma capsulatum
ABBT01000064.1 Ajellomyces capsulatus G217B
55% to CYP65V4
see fungal pages for seq
CYP65V6 Aspergillus terreus
57% to CYP65V5
see fungal pages for seq
CYP65W1 Nectria haematococca (Fusarium solani group)
JGI gene model e_gw1.5.258.1
Necha1/scaffold_5:405215-406714
49% to CYP65B1 N. crassa
see fungal pages for seq
CYP65W2 Aspergillus niger
fgenesh1_pg.C_scaffold_14000165|Aspni1
53% to CYP65W1
see fungal pages for seq
CYP65X1 Aspergillus oryzae RIB40 genomic DNA, SC023
GenEMBL AP007157.1c, BAE58707.1
join(331674..331760,331832..332607,332687..333038,
333095..333264,333318..333474)
third P450 gene in a set of 8 on this accession
42% to CYP65S2, 40% to CYP65S3, 42% to 65A1
MALAAEYFSHALSQPYGSSFWVAVAASLVFICYITCDTVYNLCL
SPLSSFPGPRLWAVSNIPRQLSILGGRSHLKMLALHHRYGPVVRVGPSELSFNSPQGF
RDIYGFRRGQPQFQKDPKMYGSPLTGISNSIGGHVDSDTHSRHRRLLSHSFSERSLRE
QEGIVVYYVDLFIQRLRERTSVNKIHRAEEDLKSWFNFTTFDIIGDLMFAETFDCLKD
SQLHPWIALMFNNVKGIAFLGVLNEYSLFRKMQGALLPKALKQKMLENHKLCAQKADR
RLQKGASRPDFVSMQTQSCKHMGPLLFFFFMAANTDSITIAGSETTASLLSGCLFYLC
KHKYIMDQLNKEIRTTFSKDEEITSSKCFNLSYLNAVLKESLRLYPPVAASLLRLVPK
GGCTIDGHFVPEDVTVSTHHYASYRDAANFTFPEQFIPERWLGTDNRFDSDRKDVVQP
FSLGPRDCLGKNLAHMEMRLILSKLLFNFDIHLTPESENWGQQKMFIVWDKPALMVRL
TDRFA
CYP65X1 Aspergillus flavus
99% to CYP65X1 Aspergillus oryzae, 48% to CYP65AU1
see fungal pages for seq
CYP65Y1 Aspergillus fumigatus Af293
GenEMBL XP_746900.1
49% to CYP65V1, 88% to CYP65Y1 Neosartorya fischeri
revised 3/12/2009 added N-term, one stop codon
MGVGSDKG*VVCYVITQAVYNVFLHPLSRFPGPLLHRASRLAYVYRLIGGTLTLDALE
MHQRYGDIVRVAPDELFFAHPDAWSDIHKKTGREMDKAPWFYRPLEQSPRCIVDESHEQHSRLRRQMAPG
FSEKSMRDQEPIIRGYVDLLLQRLLEKSNDGQPVVISDWFNYTTFDIIGDLVFGEPFECLGSSKYDGWIK
SIFESLRPLTIVQALSFFPWLKRIALDTVPKNLQGDIKQQQQQTEAKMRRRMAVTASRGDLMEGLLKKKD
ELHLSTEELIGNAQVLILAGSETTASLLSGVIYLLLQNAVAYRQLVDEVRSTFNSEKDINFVSVSQLSYM
LACLNEALRIYPPVANGLPRVVPKGGAQILGQYIPEQTYVAIPQWALYHREQYFAEPNDFHPERFLGDPK
FANDRRDALQPFSVGPRNCLGRNLAYAEMRLILALVIFHFDMKIDPDCRDWIQQRNFTLWQKPPLKVYLK
PVARKSEPNQV
CYP65Y1 Neosartorya fischeri
88% to CYP65Y1 Aspergillus fumigatus = ortholog
see fungal pages for seq
CYP65Y2P fragment Histoplasma capsulatum G217B
C-term pseudogene of CYP65Y sequence 67% to 65Y1
ABBT01000195.1 Ajellomyces capsulatus G217B
see fungal pages for seq
CYP65Z1 Aspergillus fumigatus Af293
GenEMBL XP_751828.1
41% to 65R1 Fg, 44% to 65S3
MAPWNHDIFDRLMLSAILSWSILLQSQIANYRRRTLTRRQGCVYLIGHSIYAIYFHPLSRYPGPKIAAIS
PIALLVWEIRGKVHSKVKHLHDRYGDVVRIGPNALVYRAPRAWKEIYGYRTKKGQRTFQKDPSLYVPTPN
GVHAIITAAESDHIRMRRLLAHAFSDRALREQESLLHFYADLLVQKLHENLNHSHSEVVDIARWYNFTTF
DLIGDLAFGEPFQCLKDSKYHWWVSIMLDAVKLSVYLKVLWFFPILSPLTKLLVPRYLHQRREASFQLTV
EKVSRRLHRQTNRPDFISYILRHKDDENRMSRQEIDANAATFVLAGSETTAALLSGCTFYLLKNPHIYRR
LVTEIRSRFQHPSEIRLSSIATLSYLNAVLEESLRIYPPIPAMLPRLVPEEGAMINGEYVPAGTSVSMSL
WSTFHSSGNFHNPDSFVPERWLASPEEEASRCFTMDKKEAFQPFSYGPRNCLGQHLANAEMRLILAKVFW
HSDMELCPESENWADQESYNLWSRPPLMVMISRANGRNYEDNMV
CYP65Z1 Neosartorya fischeri NRRL 181 XM_001267015
94% to CYP65Z1
name revised from CYP65Z3
see fungal pages for seq
CYP65Z2 Neosartorya fischeri NRRL 181
XP_001260852.1
56% to CYP65Z1 Aspergillus fumigatus Af293
76% to CYP65Z5 Aspergillus clavatus
MSPISIVLSILVAVLLGQAIYNIYLHPLRKFPGPKLAACSNICY
IWWTISGEIHAKLKELHDQYGDVVRISPSTLVYRSASAWKDIYGHRKHGASSFIKDPE
YYIPSPNGGHILTAGDADHARQRRLLSHAFSEKAMREQESLVQHYVDLLVEKLRAECA
ASRSTVDMMKWYNYTTFDIIGDLAFGEPFDCLRDNRYHPWVKMVFQSVKMSVYLRLIH
LYPLLESLVKRLLPKKAAKMRNEHWRMSKDKVSRRLDLQTSRPDFMSCILRHNDERGM
TRQEIEANAGVLILAGSETTATLLSGCTFYLLKHPEKYNKLVQEIRGAFQKQDDITFL
SVARLPYLHAVLEESLRLYPPVPGYLPRKVPMGGASIDGQYVPGGVSVSVVPFSASRA
KSNFAEPESFIPERWLENRDRRFETDNREASQPFSFGPRNCLGKNLAYAEMRLIVAKL
LWNFDMTLHEDYRDWDDQTSYIIWQKPSLMVELKAVR
CYP65Z2P Aspergillus fumigatus
87% to CYP65Z2 Neosartorya fischeri
supercontig 2 3641455-3643345 region
see fungal pages for seq
CYP65Z4 Aspergillus clavatus
81% to CYP65Z1 Neosartorya fischeri
see fungal pages for seq
CYP65Z5 Aspergillus clavatus
76% to CYP65Z2 Neosartorya fischeri
see fungal pages for seq
CYP65AA1 Aspergillus fumigatus Af293
GenEMBL XP_749877.1
43% to 65S2, 40% to 65Z1
MSFLMESSAVGQPSPLSFSNTLNALPGALVLYAALHSLYNIYLHSLSRFPGPKLWQTSYIFRHIASIRGT
LDVSIKAFHTEYGPVVRYSPDELSFISAEAWRDIYGFRENALPKDPSFYGLIQLSRDKSPSIFTVDQDHH
PRVRKALSYAFSEKALHDQEPFVKHYVDLLIQRLRGIADAEDNRVDLVKWYNFTTFDIIGDLAIGRSFDC
LQDSAYHSWVDAFWKSIKMISPYARAMATYTDVPRLLRLFAPRALKEARLRRLQYVGVHMEERLARGILR
DKPDFISYILRSKGTADELTDGEVEANVSFLLLAGTETTATALSGTTYYLLKNPEGLRKATAEVRSAYNS
EDEITFATTAERLPYMQACLTEGLRIYPSGPIAAPRRTPRGTVTWIAGHPVPPVGGASNSPLNFHRQADF
IPERWLSPSTMDPASPFRRDNRAASQPFSVGPRNCLGKAFALNEMQVILARMLWNFDMKLLPQSDGWERQ
RIFTLWDKGPLMVELNEVRSSLHNQLFLQVCMVVCMVVHQSRSLQRAVHSSGPARNLTG
CYP65AA1P Neosartorya fischeri
NFIA_074000
C-term is missing
92% to CYP65AA1 Aspergillus fumigatus
downstream gene is a polyketide synthase
both genes are broken and fused at YNSE/PVEP
PVEP is about aa 410 in the A. terreus PKS gene XP_001215858
The high conservation (92%) suggests an assembly error rather than a
deletion creating a pseudogene. CYP65AA1 in A fumigatus is upstream of
a short PKS gene on AAHF01000007
CYP65AB1 Aspergillus oryzae
GenEMBL BAE56591.1
41% to 60A2, 39% to 60A5, 40% to 60B3
43% to 65S1, 40% to 65A2, 41% to 65B1, 44% to 65H1
47% to 65S3
Supercontig 5: 63753-65569 (+) strand
Revised 3/18/2009
MLFSLGPLTIVYGLVIFVVAKTIYNLYLHPLRSYPGPLLARATRWYYSYYVKIGLLPQKTKELHDQYGPC
VRIAPDELSYNTAEAWEDICGHRTGQRTESFEKDLTFFPPAPNGVDSI
IVAKDDVHRRFRRLLSHPMSDKA
LGSQQEIITGYVDQLIHELRQRSERSEVVDMVRWFNFTSFDILGDLAFGESFGCLGSGLMHPWIELIFTS
IKSVMDMQIIRRIPGLFSLILTIAGLQQKQDLQEQFMFCQKKARERYTKETTRPDFMTYILRATEEKGMT
PEEIEANAQILIMAGSETTASALSGTLFYLLKNSMAMQKLRQEIHATFQAEAEITMRSTQSMEYLHAVLQ
EAMRVYPPVPCTFPRTTPPGGAMVCGRFVPGGYIVGVNQLAAMTSEKNFKDPLKFIPERWCGDERYQEDS
RKAYQPFSYGPRNCLGKNLAYAEMRLVLTRLLWNFEFDLLEESKDWHAKQKVWMMWDKGDLKVRLKPLRH
CYP65AB1 Aspergillus flavus
Supercontig 8: 73235-75051 (+) strand
see fungal pages for seq
CYP65AB2 Aspergillus clavatus
82% to CYP65AB1 Aspergillus oryzae
see fungal pages for seq
CYP65AC1 Aspergillus oryzae
GenEMBL BAE59505.1 AY510452.1
58% TO CYP65AC2, 45% to 65U1
MSQFAREIVRNAIYNTSSPDADSVSLRKATTTVLLIGVT (0)
YCILVGIYRVTLHPLAKYPGPKLAAVTRLWHSYHLCTGDIVSVLSRAHEAYGPVLRIAPD
EVLFISSRAWDDIYGARPGKPE
MDKDTPLYKGPTAPHSIVTVDGELHRFYRRLLAKGFSDAALREQEPVIQRNINLLVEKLHKEVAAGKTPE
MTAWFNYATFDLIGELAFGETYGCLENSHYHPWVEMILEVMKLRAMTHAVGYYPWIFHILMWFVPKSLRE
KFVTHRRYTHDKVQRRMDQKIHYKDLTTNLVDPQNGLERYEIDGNCSTLIIAGSETTATALSATLYFLTQ
NENAKRKVIGEIRTTFNNAGDINSISVNQLKYLSACMNEALRIFPPGPAVFPRRVPQGGDFIDGHWIPGG
TQVGIAHYCINRSRRNFVDPDKFIPERWLGDPTYQTDDRHAVQPFSYGPRNCIAHNLARLEMRLVLARLI
WEFDWELAPGSERWEEEALVFNVWSTKPLMIKFTPVAR
CYP65AC2 Aspergillus oryzae
GenEMBL BAE54634.1, AP007150.1
40% to 60A2 (CYP60 is in the 65 family)
revised 3/20/2009
MTLSAESSQLPWALGNIVQQVHGIPIWGTVLTILVLVVL (0) HYAIYAVYNITFHPLAKYPGPKVAAMTRIVYTSHLLSGRLVEFLHQTHLKYGAVVGVAPN
EVIFTSSQAWNDIYNVRQGAPE
MSKDTPLYQSLGTPPTIAEAGHDLHRRYRRLLAKGFSEAGLREQEPVIQQKISVLIKQLHAATAKGTTPE
MTSWFNFFTFDLISELTFGESFKCQENGRFHPWILMVTQSIKFRAIIQALGYYPLLFKLFMGLIPKSYQE
AFRDHQKLTSENVQRRIDKKVDYTDLASNLIDPKHNLERYEIDGNCAVLIVAGSETTTTALSATMYYLTQ
NPEAKAKVIKEVRSSFSSAEEITAISVNQLKYLPACFNEAMRKLPPAPAVFTRRVPREGAYIAGNCIPGG
THVGMCHFATNNSSLNFKDPEKYIPERWLGDPEYEDDARAAMQVFSVGPRNCIGQNLARLELRLLLSRVI
WEFDWELDSASMDWDKDMPVYLSWGMKPLKFHFAPVVR
CYP65AC2 Aspergillus flavus
98% to CYP65AC2 Aspergillus oryzae
see fungal pages for seq
CYP65AC3P Aspergillus nidulans
67% to CYP65AC1 AN10101.3 (partial)
ETYGPILRIAPGEVLFTSSSAWDDIYGARPGKPEMDKXXXXX
KGPIAPHSIVAVDGELHRFLRRLLAREFSDVKLREQEPVNQRSINLLIEKLHDEVAAGKTPEMTAMFN
YATFDLIGELAFGETYGX
MENWRYHPWVKMIFYVMKLRALTHAVGYCSWVFPILXXXX
PQVLRD*FVVHRKYTTYDKVQRRKDRKIHYWELTTNLFDPQTGLER
XXXXXXXXXXXXXXXXXX
STALSATLYFLTQNEDAKRKVIGEXXXXXXXX
GDIKSISVTQX
KYLHTRVNETLQIFPPRSAVFPRRVPLGGDWILGGTKVGSVHCCISCCRWNFV
DPDKFIPERWLSAPAY*NDDRRAMQAFKSSSLHFS*SLRLKMRLVLARLICEFDLELA
PGSE*WEGALVFNVWGTKPFKI
CYP65AD1 Aspergillus oryzae
GenEMBL BAE55230.1
45% to 65V1, 43% to CYP65AF1
MFPCCLVPTISTSQPVEVSIAAIASWSYNVFFHPLSNYPGPRLAAATRLWYAWHCAKGSLPFAIHKLHLR
YGDVVRVAPDELSYIHPDGWNEIYGHRPGQSEIPKDPSFYSSALSSPEGIFRAPRDRHGYIRRQMSHGFS
EKSMREQEDTIRHYADLMISYLSTQANGPKENVVDFTRWYNYFTFDVMGQLVFGESFNCLQSSGFHPWVS
IIFDSIRYNVFVRCTQFWPWLSPVIRRFIPKSFQRRKIEQQALSREKANYRKTIHDGRNDLVANLLKPDS
GVTDLEYQSTVQTLIVAGSETTASLLCGVTFHLLNNPEKLEKAVKEVRSEFDSADKISFVSVNKLHYLLA
CLNEALRVYPPVADGFPRRTGSNVEVINGQPVPPNTAIRMTHWATYRSPRNFVRPNEYLPERWLGNAPGF
EKDHKNALQPFHVGPRNCIGRNLAYMEMRLLLALVLWNFDLELYPASKLWDKQRVYNLWEKPELKVKVLP
RKI
CYP65AD1 Aspergillus flavus
see fungal pages for seq
CYP65AD2 Aspergillus terreus
AAJN01000140.1
60% to CYP65AD1 Aspergillus flavus
see fungal pages for seq
CYP65AE1 Aspergillus oryzae
GenEMBL BAE60010.1
40% to CYP65AF1, 39% to 65S2
MEALPTYLESFWHSGGGTMGISILVMLSTFLALGTIFVYRIWLHPLSGFPGPKCCSVSSIPVAWAQLRGR
NHEFVSSLHRKYGSVVRISPSELSFISGAAWNDIYSRSKGRPALERDRTFFNDMLVDPETITMANEATHS
RIRRAMAPAFSPRALLEQEPIIQANIKLLMDKLEARAGSGGAPTDLRAWFNYTTFDLIGDLAFGESFGCL
ATSTCHEWVQFVLDHFYTSTLLHVVHRFHPFNRVLAALLPKSLIEKRKAHDSMTLTKVHRRLEVQGRRND
FTQHLLDAAEAGTLSSREVEKQASVLILAGSETTSVALTFAIYLVLTNKPVLDQLNDELHSTFKEEQEIN
LLSVNQLKYLHAVIQEALRFCPPISNGFPRQTPPQGATVDGMFIPGKTVVNINHWAAYRSPRNFTLPEQF
LPERWLGDPRFDEDAKDVFQPFSVGPRNCIGKKFAYDSMKLILAKFLWRFKPTLLDKSRSWLAHQPTFVS
FHQPPLLVDLEIKGSDAFPVRE
CYP65AF1 Aspergillus oryzae
GenEMBL BAE65144.1, AP007171.1
46% to 65T1 50% to 65S2
14 P450 genes and 2 pseudogenes on this contig
MLSFLPLPGPFSSPVGTVGFYLGLGVLL (0)
SILYLFAVIIYNVYFHPLAKFPGPKSYAATRIPYFQALLGGQIGQAIKDLHQK
YGEVVRIAPNELSFIDGEAWKAIYGTRPGHKQKPKDVRYYPPTAGGVPSIVISNDEDHSRFRRTLSHAFS
ETSLRVQEPLVNSYIDLLIQRLHEHCEAGNKPLDMVAWYNFTTFDIIGDLAFGEPFNCLQNSAYHKWVSM
IFSNIRYGTYGNLARRFPGSKFLLRLITPTRIANGRNWHIELTKEKVKDRLAKSNDRMDFYGHILKQKDT
ERAMTFDEMVTNGSTLIVAGSETTATLLSAVTFYLLKNERVLSKLQQEIRASFESEKDITVTGCNQLEYL
NAVLTEGLRIFPPTPTGLPRIVDADGDMIAGKWVPGGTIVSIPHLAAFHSASNFTEPESFIPERFLGDPR
FANDSKTVLQPFSFGPRNCIGRNLANAEMRLILARVLYNFDLELDERSENWSRQETYILWNKPGLYVRLR
PRVGI
CYP65AF1 Aspergillus flavus
98% to CYP65AF1 Aspergillus oryzae
see fungal pages for seq
CYP65AG1 Aspergillus oryzae
GenEMBL BAE60004.1
45% to CYP65AF1, 39% to 65U1, 47% to 655Z1
MSRRFPIQVAQSEIPPIAWGLWRMVYRLWLHPLSGYPGPRLAAVSNLPYFAWTCTGNLHLRLQELHKVYG
DVIRIRPNALTYRTPEAWTDIYGHRKPGTLPFSKDPEFFMPAQAGSSHMINANEKDHTRQKRLLNHAFSE
RSLRQQEHLIMGYIDLFIQRLRGQARMGAETVNMEEWLNFLTFDIIGDLAFGEPFGCLQNSEYHPWVATI
FKSIKTGAILRALNIYPILLGFIRRFLPKSLVQKRIAHYQMSKDRVTRRLQTETSRPDFISYILKYNDDR
GMSTPEIEMNAALLIQAGSETTATVLAACLYFLQKNAACHRRLVQDIRSAFTQETDINFLSAAQLPYMNG
VIEESLRLFPPAPGIGPRVVPKGGARICGRYVPGGVSVSVGHYSTFRSARNFTRPNEFLPQRWLDRDAES
EFASDQTMALQPFSYGPRACIGRNLAYAEMRTILAKILWHFDVQLDERSADWANSKSYIVWEKGPLWLKL
HPRNVPQETD
CYP65AG2 Aspergillus niger
JGI gene model e_gw1.3.1457.1|Aspni1
57% to CYP65AG1
see fungal pages for seq
CYP65AG3 Aspergillus terreus
64% to CYP65AG1
see fungal pages for seq
CYP65AH1 Gibberella moniliformis (also Fusarium verticillioides)
AF155773.4
fumonisin biosynthetic gene cluster,
FUM2
47% to 65L1, 45% to CYP65R1
MYQITAICDARWWLCLAFTCMAMLALFSFGVVMYNIFFHRASKF
PGPTIAGATSFRYHWAMSTGNVAPWLREQHARYGEVVRIAPDMISYVSPDAWKDIYAY
KPGEKEQNGIDWTIPSRDDDVPSMFSEPNDAEHNRVRRLFLPAFSDRALKQQEPLLSK
YSDQLVHLIRRGIDDNRDQEFDAVKLYNFTTFDIMGDLTFGEPLGLLKNSSYSEWVQN
LFRDIKTAGIFLFIFDFPPLPWLVKKFSPPSIQRAHEIHKQHTVDRVNRRLEKGLDRP
DIWNLVLSQPEGRGLTHPQMHANADIFMIAGTETTATLLSGLTYLLLKNPEKLQRLVE
EIRGSFGSIEELTVENLARLPYLSACLSEGLRCYPPVPIGPSRVTPKTGGQVLGERVP
GRVRLTIPQCAAYYSDLNFKDPYSFIPERWLPGTGYESDRKGILQPFLIGPRNCIGQN
LAYHEMRLILCKLLWHYDIELCPDKGNWLLDQKMYIFWEKRPLMVKFTKARK
CYP65AJ1 Alternaria solani
Alt1 BAD83680 48% to 65J1 Mg
PKS for alternapyrone is next to three P450s called alt1, 2, 3
Chem Biol. 2005 Dec;12(12):1301-9.
1 MPAASELERR TKRSKSRNKI SKPIANMLSV IAFSICISPI VYFLIRSIYY LVFHPLSDYP
61 GPKLWAISRI PWNYVNLQGD LAWRIRDMHL HYNSSVIRIA PDELSYTSST ALKKIDGTPP
121 PREFLKCLDG RGIAPAVVNR RRSIVTETPE RHTILRRALQ PAFSERALRD QEDFFRDHTD
181 RLIAQLRKPQ YGVTEQNILR WFALLSFDIM SDLAFGQPAG CLDLVDEPWL GVIGSRVKSI
241 VWYQFAVYYR IEWILKWIMP KAAMEARKRH QALTLQKVQR RIEEERSGKR EGKKRDFMSY
301 ILGNDKENLS NMDLFGMASA FIVAGSNTTT YTMTAFTFFV CRDSEVYAKV IAEVRDKFAS
361 DTDITMVAAG DLPYLKACIE ETMRLSPPTP SALPRWVLEG GEEIDGKWVP GGVTVGVHNL
421 AACHVPWNWH RPLEFIPERW LQTKEGEFTH DDRALHARFL MVDIMSLTCF LSSMAMNEMR
481 LALAKLFWNF DISLSRNSGN WWITQKSYLV WEKKPLMVTI KPRH
CYP65AK1 Aspergillus niger
JGI gene model fgenesh1_pg.C_scaffold_9000016|Aspni1
42% to CYP65R1, 34% to fgenesh1_pg.C_scaffold_5000711
see fungal pages for seq
CYP65AL1 Aspergillus niger
JGI gene model fgenesh1_pg.C_scaffold_5000711|Aspni1
42% to CYP65Z2, 43% to CYP65Z3
see fungal pages for seq
CYP65AM1 Aspergillus niger
JGI gene model estExt_Genewise1.C_31719|Aspni1
45% to CYP65AF1, top 28 hits are CYP65 or CYP60
see fungal pages for seq
CYP65AM2 Mycosphaerella graminicola
65% to CYP65AM1 A. niger
see fungal pages for seq
CYP65AM3 Aspergillus clavatus
92% t0 65AM1
see fungal pages for seq
CYP65AM4 Mycosphaerella fijiensis
62% to CYP65AM2 Mycosphaerella graminicola
JGI gene model estExt_fgenesh1_pm.C_10205
see fungal pages for seq
CYP65AN1 Aspergillus niger
JGI gene model gw1.16.109.1|Aspni1
36% to CYP65Z1 top 26 hits are to CYP65 or CYP60
see fungal pages for seq
CYP65AP1 Aspergillus niger
JGI gene model gw1.10.1058.1|Aspni1
40% to CYP65AF1, 60% to fgenesh1_pg.C_scaffold_1001144
Top 27 hits are CYP65 or CYP60 (CYP5118 also in this set, may need name
change = 41% to CYP65Z2)
see fungal pages for seq
CYP65AP2 Aspergillus niger
JGI gene model fgenesh1_pg.C_scaffold_1001144|Aspni1
40% to CYP65Z2, 60% to gw1.10.1058.1
see fungal pages for seq
CYP65AP3 Histoplasma capsulatum G217B
ABBT01000035.1 Ajellomyces capsulatus G217B
68% TO CYP65AP1
see fungal pages for seq
CYP65AP4P Aspergillus terreus
some parts are 76% to CYP65AP1 Aspergillus niger
see fungal pages for seq
CYP65AQ1 Aspergillus niger
JGI gene model gw1.10.1062.1|Aspni1
37% to CYP65Z2 top 36 hits are CYP65
see fungal pages for seq
CYP65AR1 Aspergillus niger
JGI gene model gw1.11.201.1|Aspni1
36% to CYP65AH1, top 21 hits are CYP65
see fungal pages for seq
CYP65AS1 Mycosphaerella graminicola
39% to CYP65E1, all top hits except CYP5117A1 are CYP65 sequences.
This may mean that CYP5117 should be renamed.
N-term from JGI model estExt_Genewise1Plus.C_chr_11525|Mycgr3
see fungal pages for seq
CYP65AT1 Mycosphaerella graminicola
JGI gene model e_gw1.3.1373.1|Mycgr3
40% to 65L1, 41% TO 65Z2
see fungal pages for seq
CYP65AU1 Coccidioides immitis
47% to CYP65X1
see fungal pages for seq
CYP65AV1 Histoplasma capsulatum
39% to CYP65AZ2, 40% TO CYP65AF1
see fungal pages for seq
CYP65AW1 Fusarium oxysporum
44% to CYP65A1 Fusarium sporotrichioides
see fungal pages for seq
CYP65AW1 Fusarium verticillioides
90% to CYP65AW1 Fusarium oxysporum = ortholog
see fungal pages for seq
CYP65AX1 Fusarium oxysporum
42% to CYP65B1
see fungal pages for seq
CYP65AX1 Fusarium verticillioides
94% to CYP65AX1 Fusarium oxysporum
see fungal pages for seq
CYP65AY1 Aspergillus flavus
40% to CYP65S4 Fusarium verticillioides
Note: there is no ortholog of this gene in A. oryzae
see fungal pages for seq
CYP65AY2 Penicillium chrysogenum Wisconsin 54-1255
AM920421.1
61% to CYP65AY1
MQLLEWVVALTGLVWHTQGSFTPSLEQSTNLVQ
364847 WIISHLCLAIYNVFFHPLRNYPGPKLDAATGLVYVYHMVRGNSCKYLAGLHEKYGEVVRA 365026
365027 GPNEISYMTVSANKTIFGNKTTEKMSFEKNPAVYIQGEGTAQNILFASTGEHPRFKKLMS 365206
365207 PAFSEQAIKEQEPTIQQYTSIMIDALRNNRSGEACYPDANGIANIGAWCNFLIFDILSCL 365386
365387 SFGKPIGCLQMADYHEWANVIFGAMKHSHFLQCAHRLKPYHRLLEKLIPGDISGPYETHM 365566
365567 QNVRKNLRERETMENLARADFASFIPKGMSEDELVDNVNILATAGSETTATTLSSLFY 365740
365741 YLTHNPDSYQKLVDEVRSTFSSEEEITFNAVASLKYLKAVIQETFRIHPSVPVGLHRITP 365920
365921 KAGSYIDGKWVPG 365959
365982 QTWVSVALLAAYRSPRYWKQPEDFLPERWLEDPEFASDNRQIWAPFSIGPRKCIGIN (2) 366182
LAYLNMRLIVARLLWNFDFDPQPDNVDPHELKEYGVWQG 366356
QVPLNLKIRDARA* 366398
CYP65AZ1 Aspergillus oryzae
GenEMBL BAE63025.1
39% to 65S2, 41% to 65Z2
formerly CYP5118A1
see fungal pages for seq
CYP65BA1 Aspergillus terreus
43% to CYP65AF1
see fungal pages for seq
CYP65BB1 Mycosphaerella fijiensis
JGI gene model e_gw1.18.265.1 revised at N-term
46% to CYP65AF1 Aspergillus oryzae
45% to CYP563A2 Botrytis cinerea
44% to CYP65S2 Nectria haematococca
43% to CYP65Z2 Neosartorya fischeri
see fungal pages for seq
CYP65-un1 Magnaporthe grisea
MG09636.4 poor match 39% to MG02982.4
AACU01001669 cont2.1850 PSEUDOGENE
see fungal pages for seq
CYP65-un2 Aspergillus flavus
45% to CYP65L1
see fungal pages for seq
CYP65-un2 Aspergillus oryzae
45% to CYP65L1
100% to CYP65-un2 Aspergillus flavus
Supercontig 26: 405493-406148 (-) strand
see fungal pages for seq
CYP65-un3 Aspergillus terreus
36% to CYP65AQ1
one frameshift, possible CYP65 pseudogene
30/31 top hits are CYP65
see fungal pages for seq
CYP65-un4 Mycosphaerella fijiensis
JGI gene model e_gw1.3.1109.1
46% to CYP65-un2 Aspergillus flavus
see fungal pages for seq
CYP65-un5 Mycosphaerella fijiensis
JGI gene model e_gw1.8.672.1
45% to CYP65AS1 Mycosphaerella graminicola
see fungal pages for seq
66A Subfamily
CYP66A1 Agaricus bisporus (a mushroom)
GenEMBL Z82021 (932bp) AW444023.1 adds C-term
De Groot, P.W.J., Schaap, P.J., Van Griensven, L.J.L. and Visser, J.
Isolation of develpmentally regulated genes from the edible mushroom
Agaricus bisporus.
unpublished
Note: CYP66 is most similar to CYP64
WSYINSIVANSLWNSVKERMEAGTAKPCIATAMLEDLLDDDSAE
SKEEETVRRGACANGFLGGADTTVSLVTSFFMAMALYPDVQKKAQAELDQVLGGRLPE
FSDRPSLPYVNALLKESERWQPVFPLAIAHMSSNADEYDGYYIPKGTYVIGNAWSILH
DPEFYKDPLVFNPDRFLKDGEIDPSVRDPNVASFGFGRRICPGRFFPDASLYSTVTHV
LTVFDIKPNLDENGKEIGIKPDMTDGLLSIPM
CYP66B1 Agaricus bisporus (edible mushroom)
GenEMBL ABI271707
50% to CYP66A1
partial mRNA
QLKSVRTFIRNVMESPDEFSEWIHFYTSSSIMEIIYGMKAKPED
PYVDNAKKAIEGFNEAAVPGKFLVETFPVMKHIPSWFPGAGWKRQALFWRDVNREVRV
RPFNLVKDQVNEGTATRSVCRTLIGNLPDSTAPDRIVKENIAIDTCAVSFIGAAETSH
SAARVFFMAMLMNPEVQKKGQAELDKVLNGRLPEPNDGPNLPYISAMVKETLRWQLVL
PLAVPHVAIEADEYNGYYIPKGTIVFGNSWTFMHDPEVYKDPESYMPERFLKDGKLDS
SIRDPSTAVFGYGRRICPGRYFALNALYLMIAHTLAVFDIKPALDENDNEKEFKADVT
GGMISQPVPFQCMIVPRSKAAADLIQNSDLME
67A Subfamily
CYP67A1 Uromyces fabae (fungus)
GenEMBL U81793(1849bp)
Hahn,M. and Mendgen,K.
Characterization of in planta induced rust genes isolated from a
haustorium-specific
cDNA library.
unpublished (1997)
CYP67A2 Puccinia graminis f. sp. tritici
76% to CYP67A1 Uromyces fabae
see fungal pages for seq
CYP68A1 Gibberella fujikuroi (a rice pathogenic fungus producing plant
hormones(gibberellins))
GenEMBL Y15277
Malonek S, Bomke C, Bornberg-Bauer E, Rojas MC, Hedden P, Hopkins P,
Tudzynski B. (2005) Distribution of gibberellin biosynthetic genes and
gibberellin production in the Gibberella fujikuroi species complex.
Phytochemistry. 2005 Jun;66(11):1296-1311.
Malonek S, Rojas MC, Hedden P, Gaskin P, Hopkins P, Tudzynski B. (2005)
Functional characterization of two cytochrome P450 monooxygenase genes,
P450-1 and P450-4, of the gibberellic acid gene cluster in Fusarium
proliferatum (Gibberella fujikuroi MP-D).
Appl Environ Microbiol. 71, 1462-1472.
called P450I or P450-1 GA14-synthase
CYP68A1 Gibberella proliferatum (teleomorph sexual form)
Fusarium proliferatum (anamorph asexual form)
GenEMBl AJ628021
Malonek S, Bomke C, Bornberg-Bauer E, Rojas MC, Hedden P, Hopkins P,
Tudzynski B. (2005) Distribution of gibberellin biosynthetic genes and
gibberellin production in the Gibberella fujikuroi species complex.
Phytochemistry. 2005 Jun;66(11):1296-1311.
Malonek S, Rojas MC, Hedden P, Gaskin P, Hopkins P, Tudzynski B. (2005)
Functional characterization of two cytochrome P450 monooxygenase genes,
P450-1 and P450-4, of the gibberellic acid gene cluster in Fusarium
proliferatum (Gibberella fujikuroi MP-D).
Appl Environ Microbiol. 71, 1462-1472.
Ortholog to Gibberella fujikuroi 68A1
GA14-synthase
MTNHSSSYYYEFYKDHSHTFRRSMSENTLISSCLALATCAILLS
IQWLKPQPLIMVNGRKFGELSNVRAKRDFTFGARQLLEKGFKMSPDKPFRIMGDVGEL
HILPPKYAYEVRNNEKLSFTMAAFKWFYAHLPGFEGFREGTNESHIMKLVARHQLTHQ
LTLVTGAVSEECALVLKDVYTDSPEWHDITAKDANMKFMARITFRVFLGKEMCRNPQW
LRITSTYAVIAFRAVEELRLWPSWLRPVVQWFMPHCTQSRALVQEARDLINPLLERRR
EEKAEAERTGEKVTYNDAVEWLDDLAREKGVGYDPACAQLSLSVAALHSTTDFFTQVM
FDIAQNPELIEPLREEIISVLGKQGWSKNSLYNLKLMDSVLKESQRLKPIAIASMRRF
TTHNVELSDGVILPKNKLTLVSAHQHWDPEYYKDPLKFDGYRFFNMRREPGKESKAQL
VSATPDHMGFGYGLHACPGRFFASEEIKIALSHILLKYDFKPVEGSSMEPRKYGLNMN
ANPTAKLSVRRRKEEIAI
CYP68B1 Gibberella fujikuroi (a rice pathogenic fungus producing plant
hormones(gibberellins))
GenEMBL Y15278
Malonek S, Bomke C, Bornberg-Bauer E, Rojas MC, Hedden P, Hopkins P,
Tudzynski B. (2005) Distribution of gibberellin biosynthetic genes and
gibberellin production in the Gibberella fujikuroi species complex.
Phytochemistry. 2005 Jun;66(11):1296-1311.
called P450II 38% identical to CYP68A1
CYP68C1 Fusarium sporotrichioides (fungi)
GenEMBL AY040587 (not released yet)
Andrew Peplow Isaac Meek
Clone name TRI1
Submitted to nomenclature committee July 25, 2001
Low 40% range with 68A1 and 68B1, 44% to 68D1
CYP68C2 Fusarium graminearum
FG00071.1 AACM01000004 FGcontig1.4_scaffold1
See fungal pages for sequence
CYP68D1 Neurospora crassa
GenEMBL AL355928.2
Neurospora crassa sequence contig 1.317 (supercontig 36)
lovA
sequence below not annotated in genbank entry
46% to 68C1
MANPSATPSSIPSWMERLDIKSITDPSATPFSYLVTAFLLAVVVYSLQGPRFPKNIKHLN
PKGPLEFSDTRPKKEFVYGSRQMLANWFKANPNKPCRVISDFGEAIVLPPRMANEIKNDD
RLSFTRWTYK (phase 0 intron)
AFHGHLPGFEGFGEASRESHIVQEVIMRDLTKYLN (phase 1 intron)
KVTEPLAQETSMAMEANLPKAANGEWSTINLRSKILPIVARISSRVFLGEELCRNEEWLK
VTQQYTIDGFGAAEDLRLWPAALRPIVHWFLPSCQRARADVRVARSILDPVLKKRRQEKA
ANGGKAEHDDAIEWFERTAKGKYYDPAVAQLVLSLVAIHTTSDLTCQVMTNLMQNPEFIA
PLREEMIQVLSEGGWKKTSLYNMKLLDSVIKESQRVKPTGVASMRRYAEKDVTLSDGTFI
PKGGFVAVSAHDMWNSEVYEQAEKWDGRRFLRMRETPGAGKENVAQLVSTAPEHLGFGHG
QHACPGRFFAANEIKIALVHLLLNYEWRLPEGSDPKIRTFGFSMGVDPSLKVEYKGRQPEIEL*
CYP68D1 Neurospora discreta
JGI gene model estExt_fgenesh2_pg.C_110241
92% to CYP68D1 N. crassa
See fungal pages for gene model
CYP68D2 Nectria haematococca (Fusarium solani group)
JGI gene model e_gw1.16.35.1
Necha1/scaffold_16:246007-247770
55% to 68D1, 48% to 68C2
See fungal pages for gene model
CYP68D3 Aspergillus niger
JGI gene model e_gw1.6.1239.1|Aspni1
60% to CYP68D1
See fungal pages for gene model
CYP68E1 Magnaporthe grisea
MG10527.4 with C-term extension = accidental fusion
AACU01001764 cont2.2024
See fungal pages for gene model
CYP68F1 Magnaporthe grisea
MG00300.4 36% to 68A1 35% to 68D1 AACU01001402 cont2.70
See fungal pages for gene model
CYP68frag Magnaporthe grisea
MG10029.4 AACU01001319 cont2.1919
62% to CYP68A1 C-TERM fragment RUNS OFF END
VACTPTHFGFGHGVHACPGRFFAANEVKIALAHILLKYDWKLA
EGSEKLEPVERGLNYVVNPNIKFLIRRRKEELDLESLEF*
CYP68G1 Magnaporthe grisea
MG03834.4 40% to 68B1 AACU01001031 cont2.748
See fungal pages for gene model
CYP68H1 Magnaporthe grisea
MG02294.4 41% to 68B1 39% to 68D1 AACU01000444 cont2.449
See fungal pages for gene model
CYP68H2 Magnaporthe grisea
MG11075.4 38% to 68B1 AACU01001898 cont2.2223
See fungal pages for gene model
CYP68H3 Magnaporthe grisea
MG00024.4 41% to CYP68B1 41% to 68D1 AACU01001352 cont2.7
See fungal pages for gene model
CYP68J1 Fusarium graminearum
FG02672.1 AACM01000132 FGcontig1.132_scaffold1
See fungal pages for gene model
CYP68J2 Fusarium graminearum
FG04717.1 AACM01000196 FGcontig1.196_scaffold3
See fungal pages for gene model
CYP68J3 Fusarium oxysporum
68% to CYP68J1
See fungal pages for sequence
CYP68J3 Fusarium verticillioides
95% to CYP68J3 Fusarium oxysporum = ortholog, 60% to CYP68J4
See fungal pages for sequence
CYP68J4 Fusarium oxysporum
70% to CYP68J2 Fusarium graminearum
See fungal pages for sequence
CYP68J4 Fusarium verticillioides
83% to CYP68J4 Fusarium oxysporum probable ortholog
See fungal pages for sequence
CYP68K1 Fusarium graminearum
FG11002.1 AACM01000457 FGcontig1.457_scaffold8
See fungal pages for gene model
CYP68L1 Aspergillus nidulans
AN8530.1 45% to 68A1 54 clan
See fungal pages for gene model
CYP68L2 Aspergillus oryzae
GenEMBL BAE66422.1
59% to 68L1, 47% TO CYP68Q1
MLGDLFESVLTNNSVTLLITVAVAAIALHLSSPKSNLPLVNDKKPWEFRFTKARKRFLANAHNLIKAGLA
KAPAFRIVTGNGKRVVLDAKYANELRSHDDLSFGLHIADNFHAHIRGFQPFKQGSNDDEIYQNAVRMKLT
QSLGNLTQPLVDETLVALQTYWTNDTNWHAIPLKSNIQKVVAQLSSRIFLGDQICRNPNWLRIAVDYTVD
AFKAAEELRLWPKAFRAIVALFLPSCRKIRAELQEAQDIIRPVLDARRKDKQAALSAGKEPERYNDAMQW
LEECAKGRSYEPAFGQLTFSVAAIHTTSDMLTQVLYDLCGRDALIQALREEVITVVQEEGWTKPTLYKLK
LMDSVLKESQRLKPISVVSMQRVATADLRLSDGTFIPKGTSLAVSSDRMWDSEIYPNPLEFDGYRFLKLR
ELPGHETSAQVVSPSPEHMGFGFGRHACSGRFFAINEVKIALCHILLKYEFKLADGSVPRATKFGFSFTS
DPTTKLMIRRRQEETVL
CYP68L2 Aspergillus flavus
97% to CYP68L2 Aspergillus oryzae
See fungal pages for sequence
CYP68L3 Histoplasma capsulatum G217B
ABBT01000111.1 Ajellomyces capsulatus G217B 382612-384420 (-) strand
57% to CYP68L2
See fungal pages for sequence
CYP68L4P Aspergillus clavatus
AAKD03000015.1 117585-116726 (-) strand mid region is 63% to 68L2
See fungal pages for sequence
CYP68L5P Aspergillus fumigatus Af293
XP_752004.1 also EAL89966.1
Internal fragment, needs revision
Pseudogene fragment mid region aa 183-260
62% to CYP68L3 Histoplasma capsulatum
74% to CYP65L5P Neosartorya fischeri
formerly CYP5088A1
KICRNPDWLRITVGYTVDTF &
LPAENLQLWPRLIRPVVAKF &
LPPCRKICWELKEATEIITPVLEERQ
CYP68L5P Neosartorya fischeri
62% to CYP68L3
Supercontig 569: 1883929-1885384 (+) strand
MAASRIATCMKDKMLTDISTQLFRLDP
ISLAGVLAISGLFFLLTLPSKA &
SIPMVNVKKLFQIRSSHIQKRYLDACKLSKTGSRK ()
APSFRVHSDNGIKTMLAPEYANEFHAHPALRFGRAITLEFHSD &
FLGD KICRNPDWLLITVDYTVDTFFAAEDLRLWPRLIRPIVANFLPSCRKIRRELKEA
PVLEERR DLEAARKANQPQKRYHDAIQLMEECAKGQHYPPAEAQMAFSIVAIHTTSDMLT
QVLLDLCANPEVIQPLREEIVAVNQEE*WKKTALYKLKLLDS
VLKESQRMKPVNI
ASMRRLALEDIKLSDGTLIPKGSSILVSSRKMWDPDAYPNPELFNPYRFLRLRETPGHET
SAQLVWPSPDLGFGYGKHACPGRFFTANEVKIALCHIILKYNFNLPDGYTPTVRKRGIS
LSADPSAKLVIRRRQEEIAL*
CYP68L6 Aspergillus terreus
63% to CYP68L3
See fungal pages for sequence
CYP68L7 Aspergillus terreus
54% to CYP68L6
See fungal pages for sequence
CYP68M1X Aspergillus nidulans
Name revised to CYP5073A1
CYP68N1 Nectria haematococca (Fusarium solani group)
JGI gene model e_gw1.41.77.1
Necha1/scaffold_41:90805-92550
48% to CYP68A1 Fusarium graminearum, 52% to CYP58D1 N. crassa
See fungal pages for sequence
CYP68N2P Nectria haematococca (Fusarium solani group)
JGI gene model fgenesh1_pg.scaffold_36000012
Necha1/scaffold_36:51153-51671
48% to 68J2 C-term only, 64% to 68N1, pseudogene
See fungal pages for sequence
CYP68P1 Aspergillus fumigatus Af293
GenEMBL XP_756138.1 also EAL94100.1
41% to 68N1, 39% to 68A1
MDFLQRILGILSSEDVAPALFASSISVLFLILSYQVLYPPSKYSAFPTINGTRWLALSNRRILAEFVTDA
QGLMRNGLEKYDIFRIISSIGPMTILHPKYTDEIHNDRQLNFMAVLAKEMFPNYPGFDLFREGTDGSTVL
QDAVKFGSSRCLGKSTQLLSDETSTLLQKLWGDEPEWHEVTAKSSVHDIIAHLSALLFYGPELCSHKEWL
EVTDEYASVGFLAARQLRLWPPILRPIAQWFLPACRRLRYLASRTRGLIEPVIAARQKEKAICYSHGRQP
PVYDDAIEWTERAAKGRPYDAAMSPLLFSINALHTTTDLLTQVILDLSTQPDLIVALRQEILSVKPQQNG
WKNASLNQLLLMDSAIKESQRLKPTESILMRRYAMDDLTLADGNKIPKGTVLGIPIFGMRDPKIYVDPDM
YDGYRFQKMRDKPGFENKCQLVSTSPWHLGFGHGIHACPGRFLAAVQVKIILCYIVAKYDFKLAGGAPPK
VQSVGIELISDTEARLAVRRRQEMVIGLE
CYP68Q1 Aspergillus oryzae
GenEMBL BAE64916.1, AP007171.1
47% TO 68L2, 45% to 68L1, 48% to 68D1
14 P450 genes and 2 pseudogenes on this contig
MDHFNLAGPESNTSITSLEWLGIKNSFTGSHWAHITGLSELHPTGFLCLIATLIIGIVHLTRGPKPTVLP
VVNPPGTFELTANRVKKEWLVDARQIIRRGFEKFPGKPFNMIAADVGLTTVLPPEYASEIRNNPSLSFVA
FMAHLFFSELPGFEPTREGMFDNDIGITVVHKYLTVNLARITEPLSREATAALKDIFTDNSEWHDANLKA
INLALVARLSSRIFLGEELCRNEEWLKITVNYTVDVMKAAERLRRVPGPLRRIVHWFLPEAQKCRDEVKR
AGKVIRPVLEKRRREKATMESEGKEALQYNDAIEWFEQMAKSQGTSYDPEVVQLFLSTVAIHTTSDLLTV
VMADLARNPEIIEPLREEISSVLRDGGWKKTSLTDMKLLDSVLKESLRLKPIAVVSMRRVAMDHLKLSDG
TFLPKGTKMAVSSHRMWDPDVYENPEQWDGFRYVNLRETPGQDKHAQFVSTSERHLGFGHGKHACPGRFF
ASSELKVALCHILMKYDFELAPGTVVQHRYSGASYYADPAIRVMLRRRNVALPSWFER
CYP68Q2P Aspergillus clavatus
~73% to 68Q1 Aspergillus oryzae
pseudogene adjacent to CYP5117A2P on opposite strand
See fungal pages for sequence
CYP68R1 Aspergillus terreus
AF141924.1, XM_001209275.1
lovastatin biosynthesis gene cluster
gene="lovA"
function="involved in lovastatin production"
39% to 68D1 N. crassa
73% to CYP68R2 gene mlcC Penicillium citrinum ML-236B
(compactin) biosynthetic gene cluster
MTVDALTQPHHLLSLAWNDTQQHGSWFAPLVTTSAGLLCLLLYL
CSSGRRSDLPVFNPKTWWELTTMRAKRDFDANAPSWIESWFSQNDKPIRFIVDSGYCT
ILPSSMADEFRKMKELCMYKFLGTDFHSHLPGFDGFKEVTRDAHLITKVVMNQFQTQA
PKYVKPLANEASGIITDIFGDSNEWHTVPVYNQCLDLVTRTVTFIMVGSKLAHNEEWL
DIAKHHAVTMAIQARQLRLWPVILRPLVHWLEPQGAKLRAQVRRARQLLDPIIQERRA
ERDACRAKGIEPPRYVDSIQWFEDTAKGKWYDAAGAQLAMDFAGIYGTSDLLIGGLVD
IVRHPHLLEPLRDEIRTVIGQGGWTPASLYKLKLLDSCLKESQRVKPVECATMRSYAL
QDVTFSNGTFIPKGELVAVAADRMSNPEVWPEPAKYDPYRYMRLREDPAKAFSAQLEN
TNGDHIGFGWHPRACPGRFFASKEIKMMLAYLLIRYDWKVVPDEPLQYYRHSFSVRIH
PTTKLMMRRRDEDIRLPGSL
CYP68R2 Penicillium citrinum
BAC20565.1, AB072893
gene mlcC compactin biosynthetic gene cluster
join(AB072893.1 :15823..16167,
AB072893.1:16234..16405,AB072893.1:16469..17508)"
note="putative ML-236B biosynthetic gene"
73% to lovA CYP68R1
note: compactin is 6 desmethyl lovastatin
1 MLGQVLLTVE SYQWVSTPQA LVAVAVLLSL IAYRLRGRQS ELQVYNPKKW WELTTMRARQ
61 DFDTYGPSWI EAWFSKNDKP LRFIVDSGYC TILPSSMADE FRKIKDMCMY KFLADDFHSH
121 LPGFDGFKEI CQDAHLVNKV VLNQLQTQAP KYTKPLATLA DATIAKLFGK SEEWQTAPVY
181 SNGLDLVTRT VTLIMVGDKI CHNEEWLDIA KNHAVSVAVQ ARQLRVWPML LRPLAHWFQP
241 QGRKLRDQVR RARKIIDPEI QRRRAEKAAC VAKGVQPPQY VDTMQWFEDT ADGRWYDVAG
301 AQLAMDFAGI YASTDLFVGA LVDIARHPDL IQPLRQEIRT VIGEGGWTPA SLFKLKLLDS
361 CMKETQRIKP VECATMRSTA LRDITLSNGL FIPKGELAAV AADRMNNPDV WENPENYDPY
421 RFMRMREDPD KAFTAQLENT NGDHIGFGWN PRACPGRFFA SKEIKILLAH ILIQYDVKPV
481 PGDDDKYYRH AFSVRMHPTT KLMVRRRNED IPLPHDRC
CYP68R3 Monascus pilosus
DQ176595.1, ABA02241.1
monacolin K (lovastatin) biosynthetic gene cluster
possible GC boundary at exon 2/3 DNKggc
86% to lovA CYP68R1
41447 MTVPTDTVSRRLQSLAWSDIKQHAPWLPSSRTLVSGFLCLILLQILYSRGRKSDLRVYNP 41626
41627 KKWWELTTMRAKREFDANAPAWIEAWFSKNDQPLRFIVDSGYCTILPSSMADEFRKMKEL 41806
41807 CMYKFLGT (0) 41830
41908 DFHSHLPGFDGFKEVTRDAHLITKVVMNQFQTQAAKYTKPLADEASATIADIFGDNK (1) 42078
42144 EWHTAPVYNECLDLVTRTVTFIMVGDKLAHNEEWLDIAKHHAVTMAIQARQLRLWPVILR 42323
42324 PIVHWLEPQGAKLRAQVRRARQLLEPIIQERRAEKAKCLAQGIEPPRYVDSIQWFEDTAK 42503
42504 GQWYDAAGAQLAMDFAGIYGTSDLMIGGLVDIVRHPHLIEPLRNEIRTVIGEEGWTPASL 42683
42684 YKLKLLDSCLKESQRVKPVECATMRSYALQNVTFSNGTFVPKGELVAVAADRMSNPEVWP 42863
42864 EPKKYDPYRYMRLREDPDKAFSAQLENTNGNHIGFGWHPRACPGRFFASKEIKIMLAFLL 43043
43044 IRYDWKLVPNEPLQYYRHSFSVRIHPATKLMMRRRDEDL* 43163
CYP68S1 Aspergillus niger
JGI gene model e_gw1.4.292.1|Aspni1
51% to CYP68L2
See fungal pages for sequence
CYP68T1 Aspergillus niger
e_gw1.3.1460.1|Aspni1
51% to CYP68L2, 42% to e_gw1.4.292.1
See fungal pages for sequence
CYP68U1 Mycosphaerella graminicola
47% to CYP68N1 Nectria haematococca,
48% to CYP68D3 A. Niger
N-term from JGI model fgenesh1_pm.C_chr_10000062|Mycgr3
See fungal pages for sequence
CYP68V1 Histoplasma capsulatum G217B
50% to CYP68N1
See fungal pages for sequence
CYP68W1 Aspergillus clavatus
42% to 68D1
See fungal pages for sequence
CYP68X1 Neosartorya fischeri
47% to CYP68V1 Histoplasma capsulatum
Note: this seq does not have an ortholog in A. fumigatus
See fungal pages for sequence
CYP69A1 Gibberella fujikuroi (a rice pathogenic fungus producing plant
hormones(gibberellins))
GenEMBL Y15279
Malonek S, Bomke C, Bornberg-Bauer E, Rojas MC, Hedden P, Hopkins P,
Tudzynski B. (2005) Distribution of gibberellin biosynthetic genes and
gibberellin production in the Gibberella fujikuroi species complex.
Phytochemistry. 2005 Jun;66(11):1296-1311.
called P450III a distant match to CYP54
CYP97E1 Skeletonema costatum (diatom)
GenEMBL AF459441
Yang,S., Wu,R.S.S., Mok,H.O.L., Zhang,Z.P. and Kong,R.Y.C.
Identification of a novel cytochrome P450 cDNA, CYP97E1, from the
marine diatom Skeletonema costatum (Bacillariophyceae)
J. Phycol. 39 (3), 555-560 (2003)
52% to 97B3, 51% to 97B2, 50% to 97B1
submitted to nomenclature committee Jan 28, 2001
this is the first full length sequence from a diatom
See stramenopile/diatom pages for sequence and a tree
CYP97E2 Thalassiosira pseudonana (diatom)
No accession
From JGI genome project
See stramenopile pages for sequence and a tree
CYP97E3 Ectocarpus siliculosus (brown algae)
Genoscope Ectocarpus siliculosus brown algae project
EST LQ0AAB42YI07FM1.SCF (C-term)
59% to CYP97E1 and CYP97E2 876 amino acids is very long for a P450
Ectocarpus sctg_63 344994-350246
CYP97E4 Phaeodactylum tricornutum (diatom)
estExt_Genewise1.C_chr_50056|Phatr2 (at JGI)
69% to 97E2 and 97E1
this gene model was automatically generated at JGI
I have not checked it, it may have some errors
MRSSDYSRAPRWESFVVLAWATASAVLVGNVFQQALPQPVQAFGPALVPHLSRRCDLIQRRVSSDLEDVDVDVDNDAILG
SNNIPRRLRIQGRGIPTRKDPIQPLDSMTYESDLIKTWEQDPSRQKGFDWEIEKLRRYFAGLRMRDDGVWVRQPSFFDFL
VSKSRSDPGNAPRPVGLVDVVKLVLTNSLTSVGLGPALGMAAVPNAVIQKYEGSFFSFIKGVLGGDLQTLAGGPLFLLLN
KYFEVYGPIFNLSFGPKSFLVVSDPVMARHVLRETSPDQYCKGMLAEILDPIMGKGLIPADPATWKVRRRAIVPSFHKRW
LNRMITLFAERAEILADDLQPKSAKGQVVDMEERFCSVTLDIIGKAVFNYDFGSVTDESPIIKAVYRVLREAEHRSSSFI
PYWNLPYADQWMGGQVEFRKDMTMLDDILADLINKAVSTRREASIEELEKRENEDDPSLLRFLVGMRGEDLSSMVLRDDL
MTMLIAGHETTAAMLTWTLFELSRGDPGLLKEVQAEVRTVLKGKERPDYDDIVAMKKLRYSLIEALRLYPEPPLLIRRAR
TEDNLPAGSSDLKSGVKVLRGTDMFISTWNLHRSPDLWENPEVFDPTRWDRPFNNAGIPGWSGYNPDKVSGLYPSENAAD
FAFLPFGGGQRKCVGDQFAMMEATVTMALMIKKYDFDFAIPAEDVGMKTGATIHTMNGLMMRARQVNENEPVQSAEGYWE
MQHLKRGLNANGRPYTTEEEAVWQTSERLSHKKEEEKPNGEGGCPMHKM*
CYP97F1 Thalassiosira pseudonana (diatom)
No accession
From JGI genome project
See stramenopile pages for sequence and a tree
CYP97F2 Euglena gracilis
GenEMBL EC678021.1 EST
60% to 97F1 of Thalassiosira pseudonana (diatom)
51% to CYP97B2 Glycine max, 50% to 97B3 Arabidopsis
52% to Pinus taeda CYP97B9, 50% to 97B4, 49% to 97B3
only 40% to 97A3 and 39% to 97C1 Arab.
This is the CYP97B ortholog
If euglena only has one CYP97, then CYP97B
May be the oldest CYP97 subfamily
IASALKNRDPADIADLEARDYDEMDNPSLLRILVDMRGEETTNQQLRDDLITLLIAGHET
TASALTWAIFELVQQPELLQRVQREVDEVLGDRLPTMDDIRGLLLCRLCIAESLRMYPEP
PLLIRRVLDDLTLPKGATAKFEATLKRATDIFLAIYNIHHDGRFWPNPDFYDPERFLRPY
KNPEIPEWKGYDPEGWKGRLYPDEVSSDYAFMPFGAGPRRCLGDVFATLEGTVALAMVLR
RFDFTFAAPTAQPD
CYP97F3 Phaeodactylum tricornutum (diatom)
e_gw1.27.30.1|Phatr2 (at JGI)
75% to CYP97F1
this gene model was automatically generated at JGI
I have not checked it, it may have some errors
MQVGKSGEEISFGDSANVLRTNIEQIYGGFPSLDGCPLAEGELADIGDGTMFIGLQNYYRNYGSPYKLCFGPKSFLVISD
PVQAKHILKDANTNYDKGVLAEILEPIMGKGLIPADPETWSIRRRQIVPAFHKAWLEHIVGLFGYCNQPLIDTLNKRVDG
DGKVEMESLFCSVALDIIGLSVFNYEFGSVTQESPVIKAVYSALVEAEHRSMTPAPYWNLPLANQLVPRLRKFNSDLKLL
NDVLDDLITRAKQTRTVEDIEELENRNYNEVQDPSLLRFLVDMRGADIDNKQLRDDLMTMLIAGHETTAAVLTWALFELT
KNPEIMKELQDEIDEVVGDRMPNYEDIKKMKFLRLVVAETLRMYPEPPLLIRRCRTPDELPQGAGREAKVIRGMDIFMAV
YNIHRDERFWPSPDTFDPLRFTRSHSNPDVPGWAGFDPKKWEGKLYPNEVASDFAFLPFGGGARKCVGDEFAILEATVTL
AMVLRRFEFSFDESKFEGKDDILSSAQGLNHPVGMRTGATIHTRNGLHLVVEKRGVPK*
CYP97F4 Ectocarpus siliculosus (brown algae)
Genoscope Ectocarpus siliculosus brown algae project
ESTs LQ0AAA4YK08FM1.SCF, LQ0AAB23YL07FM1.SCF
LQ0AAB3YI17FM1.SCF, LQ0AAB85YI22FM1.SCF
60% to CYP97F1 diatom, 62% to 97F3 Phaeodactylum
Ectocarpus sctg_63 359933-352442
Note: two digit names for lower eukaryotes end here and continue as three digit
names at
CYP501. Animal P450 families cover CYP1-49 and CYP301-499 are reserved for new
animal P450 families.
Cyp301a1 Drosophila melanogaster
GenEMBL AC017275 comp(35594-38192) also AC007356
possible mitochondrial P450 fits in the mitochondrial clan.
Cyp301a1 Drosophila pseudoobscura
See D. pseudoobscura page on this server for sequence
Obtained from UC Santa Cruz Browser
CYP301A1 Anopheles gambiae (malaria mosquito)
GenEMBL
Submitted by Christelle Abgrall, Hilary Ranson and Rene Feyereisen
See the paper: Evolution of supergene families associated with
insecticide resistance.
Ranson, H., Claudianos, C., Ortelli, F., Abgrall, C., Hemingway, J.
Sharakhova, M.V., Unger, M.F., Collins, F.H. and Feyereisen, R.
Science 298, 179-181 (2002)
Anopheles map name CYPs2l3
CYP301A1 Apis mellifera (honeybee)
WGS section genbank
Submitted by May Berenbaum 2/11/04
AmGroupUn.423b
66% to 301A1 Drosophila melanogaster.
CYP301A2 Drosophila arizonae
no accession number
from Tina Yee and Phil Danielson
submitted to nomenclature committee 6/29/99
84% identical to Cyp301a1
clone name DU36az
CYP301A1 Bombyx mori (silkworm)
BAAB01030444.1 BP117781 BAAB01134599.1 BAAB01155686.1 BAAB01102862.1
BAAB01093671.1 BAAB01132355.1 BAAB01006099.1
65% to Drosophila 301A1, 64% to Apis 301A1
CYP49 and CYP301 are nearly subfamilies of a single family
See silkworm page for sequence
CYP301A1 Helicoverpa armigera (cotton bollworm)
No accession number
Heiko Vogel
submitted to nomenclature committee Feb. 6, 2008
Contig_268 51% to CYP301A1, probable ortholog
CYP301A1 Tribolium castaneum (red flour beetle)
GenEMBL XP_974014
75% to 301A1 bee, 66% to CYP301A1 Drosophila
MVATSKFPQAAKIVKNITRSRSQTVTPAFCPHLENLNEAKPYSEIPGPKPIPILGNTWRLLPIIGQYDVS
DVAKLSELFYKEYGKIVKLSGLVGRPDLLFIYDANEIEKIYRQEGPTPFRPSMPCLVRYKSVVRKDFFGD
VGGVVGVHGEPWRRFRSTVQKPILQVQTVKKYIEPIENVTNYFIQRMMEMQNDDQEMPSDFDNEIHKWAL
ECIGRVSLDARLGCLDPNLPPDSEPQKIIEAAKYALRNVAILELKFPFWRYFPTSLWTRYVQNMDYFVEI
CMKHIDAAMERLKSKTVLDEKDLSLVERILANEPDPKTAYILALDLILVGIDTISMAVCSILYQLATRPE
QQEKMYQELKRVMPDPNTPLNTKLLDQMDFLKAFVKEVLRVYSTVIGNGRTLQQDTIIHGYRIPKGVQVV
FPTLITGSMEEFVSQSNEFIPERWMKKSSSDYKIHPFASLPYGYGARMCLGRRFADLEIQVLLAKLIRSY
KLEYHHEPLEYKVTFMYAPDGPLRFKVIKREE
CYP301A1 Aedes aegypti (yellow fever mosquito)
CYP301A1 Culex pipiens
CYP301A1 Pediculus humanus humanus (body louse)
No accession number
Reed Johnson and Barry Pittendrigh
Submitted to nomenclature committee March 14, 2007
Ph301-2, 73% to 301A1 Tribolium
Pediculus genome site
CYP301A1 Nasonia vitripennis (jewel wasp)
See wasp page
CYP301B1 Tribolium castaneum (red flour beetle)
GenEMBL XP_974040
43% to 49A1 Drosophila, mito clan
60% to 301B1 bee, 48% to 301A1 bee
MKLLRAAKRVISSSSQQQLGVISAIEENHCKEWADAVPYEEVPGPKPLPFLGNTWRFIPFIGDFQIEHID
KVSKKLYEKYGKIVKMQGLLGRPDMLFLFDPDEIEKVFRQEDTLPYRPSMPSLNYYKHVHRKEFFGDNCG
VIAVHGDRWQNFRTKVNQIMLQPRVTRMYVKSIETTSQELVDRIEVIKNKKSEVPDDFLNELHKWSLESI
AKIALDVRLGCLDPNAHPDTQTLINAINTFFMNVPILELKIPFWKIWNTPTFKEYLHALDTIREITLKHV
DIALHKLSQDDCEELSVLQRVLKMENDAKTASNLALDMFLVGVDTTSNAVASILYQLSLHQEKQAKLYEE
IASLLPCKSTQISHEKLDQMHYLKACIKETMRMYPVVIGNGRCTTRDRVIGGYQIPKGVQVIFQHCVISN
LEEYFPRSGEFLPERWLKNSELFRNHHPFASLPFGFGKRMCLGRRFADLEIQTVIAKVVRNFKIEYHHDK
LDYHIHPMYTPYGPLRLTFIRRQ
CYP301B1 Pediculus humanus humanus (body louse)
No accession number
Reed Johnson and Barry Pittendrigh
Submitted to nomenclature committee March 14, 2007
Ph301-1, 61% to 301B1 Tribolium, 58% to 301B1 Apis,
insert between PERF and Heme not in Tribolium
Pediculus genome site
CYP301B1 Nasonia vitripennis (jewel wasp)
See wasp page
CYP301C1 Pediculus humanus humanus (body louse)
No accession number
Reed Johnson and Barry Pittendrigh
Submitted to nomenclature committee March 14, 2007
Ph12, 39% to Tribolium 301A1, 39% to 301A1 Pediculus
(including Ns at N-term) and 39% to 301B1 Pediculus
39% to CYP12H1 Tribolium
Pediculus genome site
CYP302A1 Anopheles gambiae (malaria mosquito)
GenEMBL
Submitted by Christelle Abgrall, Hilary Ranson and Rene Feyereisen
See the paper: Evolution of supergene families associated with
insecticide resistance.
Ranson, H., Claudianos, C., Ortelli, F., Abgrall, C., Hemingway, J.
Sharakhova, M.V., Unger, M.F., Collins, F.H. and Feyereisen, R.
Science 298, 179-181 (2002)
Anopheles map name CYPs2l2
Note: in Drosophila Cyp302a1 is the disembodied gene (dib),
a C22 hydroxylase in the ecdysteroid biosynthetic pathway from cholesterol
Cyp302a1 Drosophila melanogaster
GenEMBL AC015396 31954-33705
Michael B. O'Connor
Submitted to nomenclature committee 11/17/99
30% to Cyp12b1 other best matches are 12 family members
the fly gene is called disembodied Dib
see <A HREF="http://flybase.bio.indiana.edu:82/.bin/fbidq.html?FBrf0101392">abstract</A>
Cyp302a1 Drosophila pseudoobscura
See D. pseudoobscura page on this server for sequence
Obtained from UC Santa Cruz Browser
Gene name disembodied dib
CYP302A1 Apis mellifera (honeybee)
WGS section genbank
Submitted by May Berenbaum 2/11/04
AmGroupUn.2216
First 3 exons
45% to 302A1 Drosophila melanogaster.
disembodied ortholog
CYP302A1 Apis mellifera (honeybee)
WGS section genbank
Submitted by May Berenbaum 2/11/04
AmGroupUn.2216
Last 4 exons
51% to 302A1 Drosophila melanogaster.
disembodied ortholog
it is highly probable that these to partial sequences are
from the same gene (missing about 68 amino acids)
Cyp302a1 Bombyx mori (silkworm)
BAAB01119552.1 BAAB01040509.1 BAAB01022270.1 CK534186
BAAB01132138.1
46% to 302A1 Anoph. Probable ortholog
See silkworm page for sequence
Cyp302a1 Bombyx mori (silkworm)
GenEMBL AB198340
Niwa, R, Sakudoh, T, Namiki, T. Saida, K, Fujimoto, Y, Kataoka, H.
The ecdysteroidogenic P450 Cyp302a1/disembodied from the silkworm
Bombyx mori, is transcriptionally regulated by prothoracicotropic
hormone. Insect Molecular Biology 2005 in press
CYP302A1 Manduca sexta (tobacco hornworm)
GenEMBL DQ357067
Rewitz,K.F., Rybczynski,R., Warren,J.T. and Gilbert,L.I.
Identification, characterization and developmental expression of
Halloween genes encoding P450 enzymes mediating ecdysone
biosynthesis in the tobacco hornworm, Manduca sexta
Insect Biochem. Mol. Biol. 36 (3), 188-199 (2006)
MYRLLNNYKCVPCRKFIQRNYTMEVSHNIIQKEPQKESLLHLND
IPGPKCYPVIGTLHKYLPFIGDYDAEALDKIAWLNYRRYGCLVREVPGVNVIHVYDPD
DIEAVFRQDDRFPARRSHLAMYHYRKNKPQVYNTGGILSTNGPEWWRLRSTFQKNFTS
PQSVKAHVECTDDIAKEFVQWVHNRKFRCNEDFLIYLNRLNLEIIGAVAFNERFDSFS
PQEQHSKSRSSKIIQAAFGSNSGVMKLDKGFLWKFFKTPLYRKLAQSQEYLEKVSTEI
LMKKMKFFESNESDNDKSLLSSFLQQPNIDQKDIIGMMVDILMAAIDTTSYTTSFALY
HIARNPECQEKIFTEVETLLPEKDSSITPNVISKAVYTRSSIKESLRLNPVSVGIGRL
LQKDITLKGYFIPKDTVIVTQNMVSCRLPQYVREPLLFKPERWLRGSSEYENIHPFLS
LPFGFGPRSCIARRLAEQNMCIILIRLIRDFQIQWKGRELGIRTLLINKPDQPVTLSF
VPRLPRRL
CYP302A1 Helicoverpa armigera (cotton bollworm)
No accession number
Heiko Vogel
submitted to nomenclature committee Feb. 6, 2008
Contig_264 49% to CYP302A1, probable ortholog
CYP302A1 Tribolium castaneum (red flour beetle)
GenEMBL XP_974252
47% to 302a1 Drosophila, 49% to 302A1 bee, 49% to 302A1 Anopheles
disembodied ortholog
MCLFSKKSVHYLLTIRTRNYYYRGRFLTTAVDPPKSFKEIPGPLSLPLVGTLYLYFPFIGRYQFDRLHKN
ALKNFQLYGPIIREEIVPGEHIVWLGDPDDIAKMFHTEGTYPYRKSHLTLEKYRLDRPHIYNSGGLLPTN
GPEWSRIRKVFQKGLSGPTEALSFIKGSDDVISEWLDTRFKKIHKETSNMDFLQELSRLFLELIGVAAFD
IRFQSFHDDELDPCSKSTKLLESAFVTNSTILKTDNGPQLWRKFETPAYRRLRKAQELMESVAIDLVALK
LSTFKEKTSNPPTLLERYLASASLDFKDIIGVVCDFLLAGMDTTTYSSSFLLYHLATNPSTQDALYEEAC
RLLPNPAAPLTTEKYKQAEYAKCAVKESLRLRPISIGVGRQLTTDVVFSGYKVPSGTVVVTLNQVLSRME
KYFPEPDSFKPERWMKNDPSYVQTHPYLVIPFGHGQRSCIARRFAEQNMVILILKLARKYKLRWNGSEID
SKSLLINKPDGPILLSFEPR
CYP302A1v1 Aedes aegypti (yellow fever mosquito)
CYP302A1v2 Aedes aegypti (yellow fever mosquito)
CYP302A1 Culex pipiens
CYP302A1 Pediculus humanus humanus (body louse)
No accession number
Reed Johnson and Barry Pittendrigh
Submitted to nomenclature committee March 14, 2007
Ph302, 52% to 302A1 Tribolium, 54% to 302A1 Apis
ortholog to dib disembodied
Pediculus genome site
CYP302A1 Nasonia vitripennis (jewel wasp)
See wasp page
CYP302A1 Daphnia pulex (water flea)
Confidential
CYP302A1 Lucilia cuprina (Australian blowfly)
No accession number
Zhenzhong Chen
Submitted to nomenclature committee Dec. 18, 2007
clone name Lce0063I12
68% identical to D. pseudoobscura CYP302a1 ortholog
only 183 aa
Cyp303a1 Drosophila melanogaster
GenEMBL AC017306 12933-14444 no introns
Cyp303a1 Drosophila pseudoobscura
See D. pseudoobscura page on this server for sequence
Obtained from UC Santa Cruz Browser
CYP303A1 Anopheles gambiae (malaria mosquito)
GenEMBL
Submitted by Christelle Abgrall, Hilary Ranson and Rene Feyereisen
See the paper: Evolution of supergene families associated with
insecticide resistance.
Ranson, H., Claudianos, C., Ortelli, F., Abgrall, C., Hemingway, J.
Sharakhova, M.V., Unger, M.F., Collins, F.H. and Feyereisen, R.
Science 298, 179-181 (2002)
Anopheles map name CYPs3r4
CYP303A1 Apis mellifera (honeybee)
WGS section genbank
Submitted by May Berenbaum 2/11/04
AmGroupUn.2253
43% to 303A1 Drosophila melanogaster. Note this sequence is
named in the same subfamily as other CYP303As since
there seems to be only one orthologous sequence per
species and it does not make sense to name orthologs with
different subfamily names.
CYP303A1 Bombyx mori (silkworm)
BAAB01045446.1 BAAB01180906.1
BP126135 BAAB01031702.1
45% to 303A1 Probable ortholog
See silkworm page for sequence
CYP303A1 Tribolium castaneum (red flour beetle)
GenEMBL XP_966541
42% to 303A1 Dm, 47% to 303A1 Anopheles, 51% to 303A1 Aedes
MLLAIGFFVIILGLLAYLDTKKPKNYPPGPSWLPIIGSAHTIAEWRKKAGYLYKATAEM
AEKYGPVIGLRVGKDLIVVxxxxxxxx
EFLASDDLAGRPTGAFFDMRTWGERR (1)
GVLLTDSDFWQEQRRFILRQLREFGFG
RKNMSSMIEEETAIMVESYQKMLAGKKSVTLNMESAFNIHILNTLWMMMAGIRYSPEDKNLKSLQDILGR
LFSTVDMVGAPFSQFPILRFIAPEISGYKLYLETHLQIWEFLYDELKRHKETWDENNLRDFMDVYLKMLN
SPERKESFCEKQLLAICLDMFMAGSETTSKSLGFCFLNLIMYPDVQKKAQQEIDAVVGRNRLPSLEDRPQ
MPYMECVVLEALRMFSGRAFTVPHRAFKDTHLNGYIIPKDAMVIANMHGTLMGPESGVEDPEIFKPERYL
KDGKITLADTYLPFGFGKHRCLGETLAKANVFLFTASLLQHFNFSIVPGSPPTTECIDGVTPGPLPFKAL
VTPRE
CYP303A1 Aedes aegypti (yellow fever mosquito)
CYP303A1 Culex pipiens
CYP303A1 Pediculus humanus humanus (body louse)
No accession number
Reed Johnson and Barry Pittendrigh
Submitted to nomenclature committee March 14, 2007
Ph303, 52% to 303A1 Aedes
Pediculus genome site
CYP303A1 Nasonia vitripennis (jewel wasp)
See wasp page
Cyp304a1 Drosophila melanogaster
GenEMBL AC008359 comp(80887-82836) also AC014292 AC009393
Cyp304a1 Drosophila pseudoobscura
See D. pseudoobscura page on this server for sequence
Obtained from UC Santa Cruz Browser
CYP304B1 Anopheles gambiae (malaria mosquito)
GenEMBL
Submitted by Christelle Abgrall, Hilary Ranson and Rene Feyereisen
See the paper: Evolution of supergene families associated with
insecticide resistance.
Ranson, H., Claudianos, C., Ortelli, F., Abgrall, C., Hemingway, J.
Sharakhova, M.V., Unger, M.F., Collins, F.H. and Feyereisen, R.
Science 298, 179-181 (2002)
Anopheles map name CYPg2r1
CYP304B2 Aedes aegypti (yellow fever mosquito)
CYP304B3 Aedes aegypti (yellow fever mosquito)
CYP304B4 Culex pipiens
CYP304B5 Culex pipiens
CYP304B6 Culex pipiens
CYP304B7 Culex pipiens
CYP304C1 Anopheles gambiae (malaria mosquito)
GenEMBL
Submitted by Christelle Abgrall, Hilary Ranson and Rene Feyereisen
See the paper: Evolution of supergene families associated with
insecticide resistance.
Ranson, H., Claudianos, C., Ortelli, F., Abgrall, C., Hemingway, J.
Sharakhova, M.V., Unger, M.F., Collins, F.H. and Feyereisen, R.
Science 298, 179-181 (2002)
Anopheles map name CYPg2r2
CYP304C1 Aedes aegypti (yellow fever mosquito)
CYP304C1 Culex pipiens
CYP304E1 Tribolium castaneum (red flour beetle)
GenEMBL XP_973180
37% to 304a1 Dm, 40% to 304B3 Aedes, 38% to 304B1 Anopheles
AAJJ01000062.1, DT790944.1 DN650948.1 ESTs
MLGFLTLLVTFVTLLVFLFYKFSKERPENFPPGPPSLPIWGSYWFLLKENYNLLHLSFETLGKRYKTDIL
GFFLGNAPTVVTLSYELTKEMLSRDEFMGRNDTIIVQTRSRGSPKGIFFTDGPVWKQQRRFALRHMRDYG
FGRRSENMENLAHQETNYLIDFLTKEPSQGDLDVCKKKGLVLVPDFMYGSLINTVMLVLTSRHFEHQEVR
KYAKAAFRFMKSEDSTGGAITITPWLRYIAPTFFGFTSAVDDNDMLIDFMK (0)
GLIEEHVETFSDDHHRDFI
DDYISESIKQGIEID (1)
23621 YLQLALITLDFMFPSPVGVGHTLSFYFAHLINNPEIQTKIQEEIDQVVGRSRLPS
LDDRKDLPYFEASIRENLRLMPVTPLGVTRRCVKDTMLGGHFIPKDSFVLANLWTAHRDPRIWDDPEEFR
PERFLDSDGKLLRKDYTIGFGAGKRLCAGETYARNTMFLIMSGLLQNFTFKSPYNRPIDLKDVLPGVTLS
LKETWISAVPR
CYP304F1 Helicoverpa armigera (cotton bollworm)
No accession number
Heiko Vogel
submitted to nomenclature committee Feb. 6, 2008
Contig_236 42% to CYP304B2 or B3, 39% to CYP304B1,
37% to CYP304C1, 41% to CYP304E1 Tribolium, 40% to CYP304A1
HAH003134 100% to Contig_236 except a short internal seq
Contig_226 1 diff to Contig_236, contig 226 and 236 agree in
Region where HAH003134 is different (frameshift?)
CYP304F2 Zygaena filipendulae (zygaenid moth, Lepidoptera)
No accession number
Mika Zagrobelny Larsen
Submitted to nomenclature committee Feb. 4, 2009
Clone name Zygae_c481
63% to CYP304F1 Helicoverpa armigera (cotton bollworm)
CYP304F3 Manduca sexta
No accession number
Yannick Pauchet
Submitted to nomenclature committee April 7, 2009
confidential
Cyp305a1 Drosophila melanogaster
GenEMBL AC018013 comp(34574-37224)
Cyp305a1 Drosophila pseudoobscura
See D. pseudoobscura page on this server for sequence
Obtained from UC Santa Cruz Browser
CYP305A1 Tribolium castaneum (red flour beetle)
GenEMBL XP_970235
41% to 305A1 Dm, 46% to 305A5 Aedes, 40% to 305B1 B. mori
MWLAVLLFFVFVVTYVIQQVKKPPNFPPGPYWLPFVGNLPQLKKLSKKLGGQHLALSHLAKEYNTNVLGL
KLGKDYVVTVFTYPLIRDVLISEEFEGRPDNFFLRLRCMGQKRGITCTEGEEWNTLRNFVNRHLRNLGFG
KKPMEKMIQDEICDILTILKKDGSDIQVDKFLAPCVLSVIWTLITGEKISRENNQLDELLDLFNLRSKAF
DMTGGTLTQYPWLRYFLPEWSGFNLIKSVNTSLKNLFMKYIREHKDGWFEGRNEDLIYRYISEMKTNTEA
SKYFTDDHLVMVCVDLFIGGAQTTSRTLGFAFLMMIMYPEVQKKVQEQIDKHYDKNSSIEYSDRYKLPYV
EAVLLETIRYRYVVPIGGPRRVTKNTTLDGYYLPKNTTVLISFYSINNDPENWQNPEIFNPERFLDEKGS
LLPDEKLIPFALGRRRCVGEILAKNCIFLLFVEILRRYNVSLAPGSKPPTGKPIAGITLSPESYRVKFTERSL
CYP305A1 Pediculus humanus humanus (body louse)
No accession number
Reed Johnson and Barry Pittendrigh
Submitted to nomenclature committee March 14, 2007
Ph305, 45% to 305A1 Anopheles gambiae
Pediculus genome site
CYP305A2 Anopheles gambiae (malaria mosquito)
GenEMBL
Submitted by Christelle Abgrall, Hilary Ranson and Rene Feyereisen
See the paper: Evolution of supergene families associated with
insecticide resistance.
Ranson, H., Claudianos, C., Ortelli, F., Abgrall, C., Hemingway, J.
Sharakhova, M.V., Unger, M.F., Collins, F.H. and Feyereisen, R.
Science 298, 179-181 (2002)
Anopheles map name CYPj2l1
CYP305A3 Anopheles gambiae (malaria mosquito)
GenEMBL
Submitted by Christelle Abgrall, Hilary Ranson and Rene Feyereisen
See the paper: Evolution of supergene families associated with
insecticide resistance.
Ranson, H., Claudianos, C., Ortelli, F., Abgrall, C., Hemingway, J.
Sharakhova, M.V., Unger, M.F., Collins, F.H. and Feyereisen, R.
Science 298, 179-181 (2002)
Anopheles map name CYPj2l2
CYP305A4 Anopheles gambiae (malaria mosquito)
GenEMBL
Submitted by Christelle Abgrall, Hilary Ranson and Rene Feyereisen
See the paper: Evolution of supergene families associated with
insecticide resistance.
Ranson, H., Claudianos, C., Ortelli, F., Abgrall, C., Hemingway, J.
Sharakhova, M.V., Unger, M.F., Collins, F.H. and Feyereisen, R.
Science 298, 179-181 (2002)
Anopheles map name CYPj2l4
CYP305A5 Aedes aegypti (yellow fever mosquito)
XM_001654548
MIVLVLTSVLIIAFSYWLLQELRRPPNYPPGPQWLPFIGNTPFV
RKLARASGGQHLAFEALSKQYNSPVIGLKLGREYVVVALQYPAVREVHSKEEFDGRPD
NFFLRLRTMGTRLGVTCTDGPFWAEHRNFVVRHLRQAGYGRQPMQLQIQNELNELIGI
IRDMDGEPVWPGSILPTSVINVLWTFTTGSRIPRDDQRLARLLKLLQDRSKAFDMSGG
VLSQLPWLRHIAPEWTGYNLLKRFNQELHEFFMIIVERHHQEYHEEKCSDDLIYAFIK
EMKDRKDDPSSTFTDLQLTMIILDIFIAGSQTTSITIDLAFMMLTMHTDIQKKIHAEI
DENLHQDEMPQQNDRTSLPYTEAFLLEVQRFFHIVPVSGPRRALSDCTLGGYQIPKNT
TILMGLRTVHMDPEHWGDPECFRPERFLSPDGKIITTERLIPFGLGRRRCLGESLARA
CMFTFLVGILQKFSLRQPANCSEKPSPKLLPGITLSPKPYKVIFEPR
CYP305A6 Aedes aegypti (yellow fever mosquito)
XM_001654549
MITLVLSSVVIVSFIFWLWQDLQRPPNFPPGPKWLPFFGNTLLI
RNLARISGGQHLAFEALSKQYKSPVIGLKLGREHVVVALQYPAVHEALTKEAFDGRPD
NFFIRLRTMGTRLGITFTDGPFWTEHNSFVVRHLRQAGYGRQPMQLQIQNELNELIGI
IRDLDSEPVWPGSILPTSVINVLWTFTTGSRIPRDDQRLTRLLKLLQDRSKAFDMSGG
ILSQLPWLRHIAPEWTGYNLINRFNQEIHEFFKATIEKHHQDYTEEKCSDDLIYAFIK
EMKERKDDPCSTFTDVQLSMIILDIFIAGSQTTSTTIDIALMILAMNTEIQRKIYAEI
DDNFHPDEIPDQNCRTNLQYTEAFLLEVMRLYQIAPIGGPRRALSDCTLGGYRIPRNT
TILMGLHTVQMDPDHWGDPENFRPERFIGPDGKIINTERLIPFGLGRRRCLGDSLARS
CMFTFLVGILQKFSLRLPDSLEGPSLKLTPGITLSPKPYKVVFEPRLK
CYP305A7 Culex pipiens
CYP305A8 Culex pipiens
CYP305A9 Culex pipiens
CYP305A10 Culex pipiens
CYP305A11 Culex pipiens
CYP305A12P Anopheles gambiae (malaria mosquito)
GenEMBL XM_001230354.2 (seq below is expanded on both ends)
Partial cDNA sequence 97% identical to CYP305A3 and CYP305A4
Chromosome UNKN: 41,132,263-41,133,123 reverse strand.
This transcript is a product of gene AGAP012937
The genomic region 66760 bp upstream of this seq
is missing the N-term 140 aa, so this is a pseudogene.
GLGRQAMHEQIQTELTELLDVLDTRAEQPLWPGSILALS
VINVLWTIVTGSRVAREDDRLQRLLELLQERSKVFDMAGGTLNQLPWLRFIAPEWSGYNL
VRRFNKQLTKFFYPTIEEHKGDFTEDKAVDDLIYAYIKEMRDREGTSNNGNNFTEVQLTM
IILDIFIAGGQTTSATLDLALMMMIVHPEVQDKVHREIDSQLEPNAIPHYDDRLKLPYVE
AFLLEVQRYFSIAPVNGPRRAVVDCTLGGYRVPKDTTVLMGLRNVHMDPEHWGDPEVFRP
ERFLNEQRQIVNTERLLPFGQGKRRCLGETLARSCLFTFFVGVMKRF
CLMGTDEGSEDGTSMAPSLTLKPGITLSAKPYHVVSQPRR
CYP305B1 Bombyx mori (silkworm)
GenEMBL AB044900
Nobuyoshi Katagiri, Okitsugu Yamashita
39% identical to 305a1
CYP305B1 Helicoverpa armigera (cotton bollworm)
No accession number
David G. Heckel
submitted to nomenclature committee Feb. 21, 2005
HaCypZ010a-v1Pep
after revision 61% to 305B1
CYP305B1 Helicoverpa armigera (cotton bollworm)
No accession number
Heiko Vogel
submitted to nomenclature committee Feb. 6, 2008
Contig_201 38% to CYP305A3
(note: original CYP305C1 seq had an error in the mid region
name changed to CYP305B1 after revision)
CYP305C1X Helicoverpa armigera (cotton bollworm)
No accession number
Vladimir Grubor
submitted to nomenclature committee 12/30/2003
55% to CYP305B1
324 aa, seq #7
revised sequence name changed to 305B1
CYP305D1 Nasonia vitripennis (jewel wasp)
See wasp page
Cyp306a1 Drosophila melanogaster
GenEMBL AC012373 154223-156412 also AC015216 AC012164 AC012376
phantom phm gene
Cyp306a1 Drosophila pseudoobscura
See D. pseudoobscura page on this server for sequence
Obtained from UC Santa Cruz Browser
phantom phm gene
CYP306A1 Anopheles gambiae (malaria mosquito)
GenEMBL
Submitted by Christelle Abgrall, Hilary Ranson and Rene Feyereisen
See the paper: Evolution of supergene families associated with
insecticide resistance.
Ranson, H., Claudianos, C., Ortelli, F., Abgrall, C., Hemingway, J.
Sharakhova, M.V., Unger, M.F., Collins, F.H. and Feyereisen, R.
Science 298, 179-181 (2002)
Anopheles map name CYPs2r6
Ortholog of phantom phm gene
CYP306A1 Apis mellifera (honeybee)
WGS section genbank
Submitted by May Berenbaum 2/11/04
AmGroup13.1b
46% to 306A1 Drosophila. Note this sequence is
named in the same subfamily as other CYP306As since
there seems to be only one orthologous sequence per
species and it does not make sense to name orthologs with
different subfamily names.
Ortholog of phantom phm gene
CYP306A1 Bombyx mori (silkworm)
BAAB01049676.1 BAAB01150476.1
BAAB01016115.1 BAAB01136488.1 BAAB01075467.1
AB162964
Niwa,R., Matsuda,T., Yoshiyama,T., Namiki,T., Mita,K., Fujimoto,Y.
and Kataoka,H.
CYP306A1, a cytochrome P450 enzyme, is essential for ecdysteroid
biosynthesis in the prothoracic glands of Bombyx and Drosophila
J. Biol. Chem. (2004) In press
See silkworm page for sequence
Ortholog of phantom phm gene
CYP306A1 Manduca sexta (tobacco hornworm)
GenEMBL DQ357066
Rewitz,K.F., Rybczynski,R., Warren,J.T. and Gilbert,L.I.
Identification, characterization and developmental expression of
Halloween genes encoding P450 enzymes mediating ecdysone
biosynthesis in the tobacco hornworm, Manduca sexta
Insect Biochem. Mol. Biol. 36 (3), 188-199 (2006)
MDLFFIWLVTFVAGFWICKKIKEWQNLPPGPWGLPILGYLPFID
PRQPHITLTKLSKLYGPIYGLKMGNIYAVVLSDHKLIRDAFAKENFSGRAPLYLTHGI
MHGNGIICAEGGLWKDQRKLITTWLKSFGMSKHSALRDKLEKRIASGVYELLDSIEKS
PKEPIDLPHMVSNSLGNVVNEIIFGFKFPSDDKTWQWLRQIQEEGCHEMGVAGVVNFL
PFVRFFSPTIRKTIQMLIRGQAQTHTLYASVIARRRNMLGLEKPKGAEHLPHSNLFNE
YPDGKLKCIKYSKHAPNTEHIFDPKTLIPTEGECILDSFLLEQKKRFENGDETARYMT
DEQLHFLLADMFGAGLDTTSVTLAWFLLYMALYPDEQEIVRKEILSEYPEECEVESSR
LPRLMAAIYETQRIRSIVPVGIPHGCLEDTYLGNYKIPKGAMVIPLQWALHMDPDVWE
DSEEFKPSRFLAPDGSLLKPQEFIPFQTGKRMCPGDELSRMLASGFIARLFRRKRVRL
ASIPSQEDMQGTVGVTLAPPRVQYFCDPI
CYP306A1 Manduca sexta
No accession number
Yannick Pauchet
Submitted to nomenclature committee April 7, 2009
confidential
CYP306A1 Helicoverpa armigera (cotton bollworm)
No accession number
Heiko Vogel
submitted to nomenclature committee Feb. 6, 2008
Contig_197 79% to CYP306A1 Bombyx
HAH005766 100% to Contig_197
CYP306A1 Tribolium castaneum (red flour beetle)
GenEMBL XP_968477, protein and AAJJ01000667.1 genomic
46% to 306A1 Aedes, 49% to Apis 306A1
Ortholog of phantom phm gene
MAPLWSVLLSFLLFIIAYTWLRRSRNLPPGPWNLPVIGYLAWLNPQFPYLTLTSLSKKYGPIYGLYLGSI
YTVVISDAKLIKKTFNKDASSGRAPLHLTHGIMKGFGLICAQGDLWKDQRKFVHNTLRTLGASKVSPNRP
TMEALILHHVSDLVQHIKSLGESVTLDPLDSLRHSLGSAINQMVFGKCWSRDDATWKWLQHLQEEGTKHI
GVAGPLNFLPLLRFLPKSKQTINFLTEGIRDTHQIYREIIEEQKNSSNETVSNVIQAFLAEKEKRKNEDS
VKFYNDQQFHYLLADIFGASLDTTLTTLRWYVLYMAVHQDVQKKVRSLLNDLTLEQIAMVPYFEATIAEV
QRIRPVVPVGIPHGSVEELEIAQYKVPPGTMIVPLQWAVHMDANIWDEPEVFKPERFINEEGKFFKPEAF
IPFQAGKRMCVGDELARMFLYLFGAALVKNFAISCMGEVDLTGDCGITLTPKPHELIFTSL
CYP306A1 Aedes aegypti (yellow fever mosquito)
CYP306A1 Culex pipiens
CYP306A1 Pediculus humanus humanus (body louse)
No accession number
Reed Johnson and Barry Pittendrigh
Submitted to nomenclature committee March 14, 2007
Ph306, phm phantom 51% to 306A1 Apis
Pediculus genome site
CYP306A1 Nasonia vitripennis (jewel wasp)
See wasp page
CYP306A1 Daphnia pulex (water flea)
Confidential
Cyp307a1 Drosophila melanogaster
GenEMBL AC014810 comp(54284-52654) also AC007840
spook spo (see Flybase)
307a1 AC014810 comp(54284-52654) also AC007840 revised 9/8/2000
sequence revised between PERF and heme, no intron is present
MLAALIYTILAILLSVLATSYICIIYGVKRRVLQPVKTKNSTEINHNAYQKYTQAPGPRP
WPIIGNLHLLDRYRDSPFAGFTALAQQYGDIYSLTFGHTRCLVVNNLELIREVLNQNGKV
MSGRPDFIRYHKLFGGERSNSLALCDWSQLQQKRRNLARRHCSPREFSCFYMKMSQIGCE
EMEHWNRELGNQLVPGEPINIKPLILKACANMFSQYMCSLRFDYDDVDFQQIVQYFDEIF
WEINQGHPLDFLPWLYPFYQRHLNKIINWSSTIRGFIMERIIRHRELSVDLDEPDRDFTD
ALLKSLLEDKDVSRNTIIFMLEDFIGGHSAVGNLVMLVLAYIAKNVDIGRRIQEEIDAII
EEENRSINLLDMNAMPYTMATIFEVLRYSSSPIVPHVATEDTVISGYGVTKGTIVFINNY
VLNTSEKFWVNPKEFNPLRFLEPSKEQSPKNSKGSDSGIES
DNEKLQLKRNIPHFLPFSIGKRTCIGQNLVRGFGFLV
VVNVMQRYNISSHNPSTIKISPESLALPADCFPLVLTPREKIGPL
Cyp307a1 Drosophila pseudoobscura
See D. pseudoobscura page on this server for sequence
Obtained from UC Santa Cruz Browser
spook spo (see Flybase)
D. pseudoobscura ortholog of Cyp307a1 Jie Shen no introns
MSAVYSVLAILLAIIITSYIRILYKSRRRVCVAIRNGKSSTESEQIYEQAPGPRPWPIIG
NLNVLDKYRDNPFEGFTELAKKYGDIYSLTFGHTRCLVVNNLELIREVLNQNGKVVSGRP
DFLRYHKLFGGERSNSLALCDWSQLQQKRRNMARRHCSPRESSSFYMQMSQIGCEEMEHF
IREIDAKMVPGEPFDVKTFLLRACANMFSQYMCSQRFDYDDEEFHKIVQYFDEIFWEINQ
GHPLDFLPWLYPFYRRHLNKIVHWSSTIRSFIMDRIISHRELDIDVDEPDSDFTDALLKS
LVEDKDVSRNTIIFMLEDFIGGHSAVGNLVMLALAYIAKNPQVGRRIQDEVDAVSSKGQR
KINLLDMHQMPYTMATIFEVLRYSSSPIVPHVATEDLVISGHGVTAGTIVFINNYVLNRS
REHWEDSHQFEPQRFLEPSTEGRRDSRGSDSGIESDQKEFQLRKNIPHFLPFSIGKRTCI
GQNLVRGFGFLLLANLLQGYNVSSGDPTTIRITPASLALPASCFPLLLTRRA*
CYP307A1 Anopheles gambiae (malaria mosquito)
GenEMBL
Submitted by Christelle Abgrall, Hilary Ranson and Rene Feyereisen
See the paper: Evolution of supergene families associated with
insecticide resistance.
Ranson, H., Claudianos, C., Ortelli, F., Abgrall, C., Hemingway, J.
Sharakhova, M.V., Unger, M.F., Collins, F.H. and Feyereisen, R.
Science 298, 179-181 (2002)
Anopheles map name CYPs1x4
Ortholog of spook spo (see Flybase)
CYP307A1 Bombyx mori (silkworm)
BAAB01102325.1 BAAB01020228.1
51% to 307A1 probable ortholog
See silkworm page for sequence
Ortholog of spook spo (see Flybase)
CYP307A1 Manduca sexta
GenEMBL DQ899315
Ono,H., Rewitz,K.F., Shinoda,T., Itoyama,K., Petryk,A.F.,
Rybczynski,R., Jarcho,M., Warren,J.T., Marques,G., Shimell,M.J.,
Gilbert,L.I. and O'Connor,M.B.
Spook and Spookier code for stage-specific components of the
ecdysone biosynthetic pathway in Diptera
Dev. Biol. (2006) 298, 555-70.
MSVLLIIAVAVYAIYKYFSKTKILWRKSNKYGKEEVEVLREAPG
PFPLPVVGSLHLLGRHESPFQAFTALAKEYGDIYSIKLGSAKCLIVNNLALIREVLNQ
NGNFVAGRPDFLRFHKLFAGDRNNSLALCDWSNLQLRRRNLARRHCGPKQHTDFYARI
GSVATLESYELVQTLKSITQTTEQSINLKPLLMTSAMNMFTNYMCSVRFDENDSEFRK
VVDHFDEIFWEINQGYAVDFLPWLAPFYKKHMDKLSGWSQDIRSFILSRIVEQREMNL
DMEGPEKDFLDGLLRVLHEDPNVDRNTIIFMLEDFLGGHSSVGNLVMLCLTAVARDPE
IGRKIRAEIEGVTKGKRPVALTDRSNLPYTEAAILECLRYASSPIVPHVATENAKING
YGVEKGTVIFINNYVLNNSEQYWNNPENFDPSRFLEKTKVRTRRNSQCDSGMESDSER
SPSKETETEVYSVKKNIPHFIPFSIGKRTCIGQTLVTTMSFTMFANIIQEFDVGVENL
DDLIQKPACVALPKDTYNMYLIPRKQ
CYP307A1 Helicoverpa armigera (cotton bollworm)
No accession number
Heiko Vogel
submitted to nomenclature committee Feb. 6, 2008
Contig_269 73% to CYP307A1 Bombyx, 56% to CYP307A1 Anopheles
HAH014257 100% to Contig_269
Submitted sequences contain an intron
CYP307A1 Tribolium castaneum (red flour beetle)
GenEMBL AAJJ01000951.1 genomic
55% to 307A1 Anopheles
Ortholog of spook spo gene
MLALLCLCVVLLVWWFSRPKKSPSTI
33251 PGPRPWPLIGSMHLLAGHETPFQAFTALSRVYGDIFSIHLGSASCVVVNNFKLIKEVLIA 33430
33431 KGGDFGGRPDFARFHKLFGGDRNN (1) 33502
34320 SLALCDWSSLQKTRRSIARTYCSPRFTSLQYDRVNNVGEEELKSFLHQLDQLPHGQPC 34493
34494 NVKPAVLMVCANMFTQYMCSTSFAYEDKGFQKIVRYFDEIFWEINQGYAVDFLPWLLPVY 34673
34674 TGHMKKISNWATEIRQFILSRIIDKHRATLDTNSPPRDFTDALLMHLEEDPNMNWQHIIF 34853
34854 ELEDFLGGHSAIGNLVMVTLAAVVDHPEVAKRIQEEVDQVTGGTRCPNLFDKAAMPYTE 35030
35031 ATILETLRTASSPIVPHVASKDTEIDGHEVSKGTIVFINNYELNQGDAYWDEPGLFKPER 35210
35211 FLSSTGNIVKPAHFIPFSTGKRTCIGQRLVQCFSFVVLATLLQYYDVSTKESVK 35372
35373 VQPGCVAVPPDCFKLVLTPRK* 35438
CYP307A1 Aedes aegypti (yellow fever mosquito)
CYP307A1 Culex pipiens
CYP307A1 Pediculus humanus humanus (body louse)
No accession number
Reed Johnson and Barry Pittendrigh
Submitted to nomenclature committee March 14, 2007
Ph307-2, spo spook 66% to 307A1 Tribolium
Pediculus genome site
CYP307A1 Nasonia vitripennis (jewel wasp)
See wasp page
CYP307A1 Daphnia pulex (water flea)
Confidential
CYP307A1 Lucilia cuprina (Australian blowfly)
No accession number
Zhenzhong Chen
Submitted to nomenclature committee Dec. 18, 2007
clone name Luce0093H16
60% identical to Dm CYP307a1 ortholog
Cyp307a2 Drosophila melanogaster
GenEMBL AC019383 comp(3284-3706) AL078186
This sequence was originally predicted as a pseudogene,
but recent work shows it is a functional gene called spookier (spok)
Ono H, Rewitz KF, Shinoda T, Itoyama K, Petryk A, Rybczynski R, Jarcho
M, Warren JT, Marques G, Shimell MJ, Gilbert LI, O'Connor MB.
Spook and Spookier code for stage-specific components of the ecdysone
biosynthetic pathway in Diptera.
Dev Biol. 2006 Oct 15;298(2):555-70.
This is a new member of the halloween genes
AC019383 comp(3284-3706) AL078186
AC019474 exon 2
MLTSVFYVLFAIAITIILISYVFLLLKCKQKAFVVIGLLYQEKKYQCFDQAPGPHPWPII
GNINLLGRFQYNPFYGFGTLTKKYGDIYSLSLGHTRCIVVNNVDLIKEVLNKNGKYFGGR
PDFFRYHKLFGGDRNN (1)
663 SLALCDWSQLQQKRRNLARRHCSPRESSSYFSKMSEIGGLEVNQLLDQLTNISSGYPCDV 842
843 KPLILAASANMFCQYMCSVRFNYSDKGFQKIIEYFDEIFWEINQGYSFDYIPWLVPFYCN 1022
1023 HISRIVHWSASIRKFILERIVNHRESNININEPDKDFTDALLKSLKEDKNVSRNTIIFML 1202
1203 EDFIGGHSAVGNLVMLALAYIAKNPTIALHIRNEVDTVSAKGIRRICLYDMNVMPYTMAS 1382
1383 ISEVLRYSSSPIVPHVAMEDTVIKGFGVRKGTIVFINNYVLNMSESFWNHPEQFDPERFL 1562
1563 ENNFTNNKESGLKCDDNKRTEFIRKNDNDGSTKSKKYGKQNLNNK 1697
1698 LLKKSIPQFLPFSVGKRTCIGQSLVRGFGFLLLANIIQNYNVNSADFSKIKLEKSSI 1868
1869 ALPKKCFKLSLRPRT* 1916
Cyp307a2 Drosophila pseudoobscura
See D. pseudoobscura page on this server for sequence
Obtained from UC Santa Cruz Browser
Contig3286_Contig7811B.fa.9 66% to D. pseudoobscura ortholog of 307A1
first exon 67% to 307A2 55% to 307A1 so this is the ortholog of 307A2
sequence revised between PERF and heme (no intron), not a pseudogene
MFFSVCYLIIAIGITAIVISYILIIFKCNEKATVRIGLSNPEKAIRKFGQ
APGPQPWPIIGNLDLLGRFKSNPFEGLGALTKQYGDIYSLTLGHSRCLVV
NSLDLIREVLNKNGKFIGGRPDFIRYHKLFGGDRNN (1)
SLALCDWSNLQQKRRNLARRH
CSPRESSPYFNKMSHIGCNEINHLMRELNKTQPGKPFDIKKLILGASANM
FCQYMCSIRFDYNDKEFRRIVRYFDEIFWEINQGYSLDFLPWMLPFYKNH
TQKIVQWSTTIRKFILDRVIHQREEMLDLDEPENDFTDALLRSLKEDQNV
SRNTIIFMLEDFIGGHSAVGNLVMLALAYIAKNPMIGRRIEHEADIVTNK
GRRRINLYDMDAMPYTMATIFEVLRYSSSPIVPHVAMEDTAIAGFGVTRG
TIIFINNYMLNMSEKYWNSPDHFEPERFLDENYRRNIWKAVKCNDN
RRGSEGSDSGIEFEKDTHVHKIIIDKLTSTKNSKKNKNIFNDKCGY
QLKKNIPHFLPFSIGKRTCIGQNLVRGFGFVLLANIVQSYNITSADISKININH
ESLALPADCFPLVLTPRA*
CYP307B1 Anopheles gambiae (malaria mosquito)
GenEMBL
Submitted by Christelle Abgrall, Hilary Ranson and Rene Feyereisen
See the paper: Evolution of supergene families associated with
insecticide resistance.
Ranson, H., Claudianos, C., Ortelli, F., Abgrall, C., Hemingway, J.
Sharakhova, M.V., Unger, M.F., Collins, F.H. and Feyereisen, R.
Science 298, 179-181 (2002)
Anopheles map name CYPs3r2
Note: CYP307B sequences have been named spookiest
Ono H, Rewitz KF, Shinoda T, Itoyama K, Petryk A, Rybczynski R, Jarcho
M, Warren JT, Marques G, Shimell MJ, Gilbert LI, O'Connor MB.
Spook and Spookier code for stage-specific components of the ecdysone
biosynthetic pathway in Diptera.
Dev Biol. 2006 Oct 15;298(2):555-70.
CYP307B1 Apis mellifera (honeybee)
WGS section genbank
Submitted by May Berenbaum 2/11/04
AmGroup14.9
55% to 307B1 Anopheles. Note this sequence is given the
same name as Anopheles 307B1 since
there seems to be only one orthologous sequence and it does
not make sense to name orthologs with different names.
Note: CYP307B sequences have been named spookiest
Ono H, Rewitz KF, Shinoda T, Itoyama K, Petryk A, Rybczynski R, Jarcho
M, Warren JT, Marques G, Shimell MJ, Gilbert LI, O'Connor MB.
Spook and Spookier code for stage-specific components of the ecdysone
biosynthetic pathway in Diptera.
Dev Biol. 2006 Oct 15;298(2):555-70.
CYP307B1 Tribolium castaneum (red flour beetle)
GenEMBL AAJJ01001163.1 genomic
53% to 307B1 Anopheles, 55% to 307B Aedes, 51% to bee 307B1
Note: CYP307B sequences have been named spookiest
Ono H, Rewitz KF, Shinoda T, Itoyama K, Petryk A, Rybczynski R, Jarcho
M, Warren JT, Marques G, Shimell MJ, Gilbert LI, O'Connor MB.
Spook and Spookier code for stage-specific components of the ecdysone
biosynthetic pathway in Diptera.
Dev Biol. 2006 Oct 15;298(2):555-70.
13728 MFFTQFTLYLIAFVVLATVFAIAEAMRTRQGKY
13629 RPPSPLKLPIIGHLHLMSGYQVPYQAFTSLSKKFGSVFGLQLGYVKCVVINGQKNIREAL 13450
13449 VTKGHHFDSRPNFERYQQLFSGNKEN (1) 13372
13324 SLAFCNWSTTQKTRRDMLKAYTFPRAFTNRFYTLEELVSSGTRSVVAALSTNTTSTKSV 13148
13147 ISRHCANIFTRHFCSKSFDVDEATFVEMVDNYDEIFYEVNQGYAADFLPFLLPFHEKNLK 12968
12967 RVNGLTHKIRDFVLDHIIEGRFDSFDVQAEPDDYVESLIKYVKSGESPQLSWDSALFALE 12788
12787 DIIGGHSAVANFLVKLFAFLAKEPQVQENIHKEIDRVLGTKEVSISHRTLLPYTEAT 12617
12616 IFEAIRLIASPIVPRVANRDSSIN (1) 12545
12489 GLEIEKGTVIFLNNYDLSMSPQLWDEPEKFKPERFIRE 12376
12375 ERLVKPEHFLPFGGGRRSCMGYKMVQLVSFGILGGIMQNFTIGADDTSYTVPVGSLALP 12199
12198 KDTFTFKFVRR* 12163
CYP307B1 Aedes aegypti (yellow fever mosquito)
Note: CYP307B sequences have been named spookiest
Ono H, Rewitz KF, Shinoda T, Itoyama K, Petryk A, Rybczynski R, Jarcho
M, Warren JT, Marques G, Shimell MJ, Gilbert LI, O'Connor MB.
Spook and Spookier code for stage-specific components of the ecdysone
biosynthetic pathway in Diptera.
Dev Biol. 2006 Oct 15;298(2):555-70.
CYP307B1 Culex pipiens
CYP307B1 Pediculus humanus humanus (body louse)
No accession number
Reed Johnson and Barry Pittendrigh
Submitted to nomenclature committee March 14, 2007
Ph307-1, spookiest 47% to 307B1 Apis
Pediculus genome site
Cyp308a1 Drosophila melanogaster
GenEMBL AC015216 72624-74273 also AC012164
Cyp308a1 Drosophila pseudoobscura
See D. pseudoobscura page on this server for sequence
Obtained from UC Santa Cruz Browser
Cyp309a1 Drosophila melanogaster
GenEMBL AC019891 comp(47437-49308) also AC009909
Cyp309a1 Drosophila pseudoobscura
See D. pseudoobscura page on this server for sequence
Obtained from UC Santa Cruz Browser
Cyp309a2 Drosophila melanogaster
GenEMBL AC009909 45541-45982 also AC019977
Cyp309a2 Drosophila pseudoobscura
See D. pseudoobscura page on this server for sequence
Obtained from UC Santa Cruz Browser
Cyp310a1 Drosophila melanogaster
GenEMBL AC017690 comp(12877-14650)
Cyp310a1 Drosophila pseudoobscura
See D. pseudoobscura page on this server for sequence
Obtained from UC Santa Cruz Browser
Cyp311a1 Drosophila melanogaster
GenEMBL AC014186 2270-3910
Cyp311a1 Drosophila pseudoobscura
See D. pseudoobscura page on this server for sequence
Obtained from UC Santa Cruz Browser
Cyp312a1 Drosophila melanogaster
GenEMBL AC015424 57845-59744 also AC009369 AC007573 AC010003
Cyp312a1 Drosophila pseudoobscura
See D. pseudoobscura page on this server for sequence
Obtained from UC Santa Cruz Browser
Cyp313a1 Drosophila melanogaster
GenEMBL AC017740 comp(167857-169728) also AC007809 AC007928
Cyp313a2 Drosophila melanogaster
GenEMBL AC017371 4100-5929 AC007724 AL057969 AL067059
Cyp313a3 Drosophila melanogaster
GenEMBL AC017371 1890-3720 also AC007724
Cyp313a4 Drosophila melanogaster
GenEMBL AC017336 153877-155807 also AC007752
Cyp313a4 Drosophila pseudoobscura
See D. pseudoobscura page on this server for sequence
Obtained from UC Santa Cruz Browser
Cyp313a5 Drosophila melanogaster
GenEMBL AC017371 6352-8145 also AC007724
Cyp313a6 Drosophila pseudoobscura
See D. pseudoobscura page on this server for sequence
Obtained from UC Santa Cruz Browser
This is a paralog of 313a4, D. pseudoobscura has two genes
To D. melanogasters one Cyp313a4
Cyp313b1 Drosophila melanogaster
GenEMBL AC007571 comp(104264-106046) also AC013225
Cyp313b1 Drosophila pseudoobscura
See D. pseudoobscura page on this server for sequence
Obtained from UC Santa Cruz Browser
Cyp313c1 Drosophila pseudoobscura
See D. pseudoobscura page on this server for sequence
Obtained from UC Santa Cruz Browser
Cyp313c2 Drosophila pseudoobscura
See D. pseudoobscura page on this server for sequence
Obtained from UC Santa Cruz Browser
Cyp314a1 Drosophila melanogaster
GenEMBL AC012699 22585-24723 also AC006496
shade shd gene
Cyp314a1 Drosophila pseudoobscura
See D. pseudoobscura page on this server for sequence
Obtained from UC Santa Cruz Browser
shade shd gene
CYP314A1 Anopheles gambiae (malaria mosquito)
GenEMBL
Submitted by Christelle Abgrall, Hilary Ranson and Rene Feyereisen
See the paper: Evolution of supergene families associated with
insecticide resistance.
Ranson, H., Claudianos, C., Ortelli, F., Abgrall, C., Hemingway, J.
Sharakhova, M.V., Unger, M.F., Collins, F.H. and Feyereisen, R.
Science 298, 179-181 (2002)
Anopheles map name CYPs2r3
Ortholog of shade shd gene
CYP314A1 Apis mellifera (honeybee)
WGS section genbank
Submitted by May Berenbaum 2/11/04
AmGroup5.25
43% to 314A1 Drosophila. Note this sequence is
named in the same subfamily as other CYP314s since
there seems to be only one orthologous sequence per
species and it does not make sense to name orthologs with
different subfamily names.
Ortholog of shade shd gene
CYP314A1 Acyrthosiphon pisum (pea aphid)
GenEMBL CF588143
48% to 314A1 Dros. Pseudoobscura aa 126-362
Ortholog of shade shd gene
CYP314A1 Tribolium castaneum (red flour beetle)
GenEMBL XP_972699, CB336479 CB336480 EST
42% to 314A1 bee, 39% to 314A1 Aedes
Ortholog of shade shd gene
MFEKIFQSLDVTSLLIIAIFFLFLEYRPPWWYRNNDCKKGVKLIPGPLALPGLGTTWIFFFGGFSFNRLH
LYYENMYKRYGPVMKEEYWCNIPVINLFEKREIVKVLKAGGKYPLRPPVEAVAHYRRSRPDRYASIGLVN
EQGEAWYDLRSTLTPALTSPKTITSFLPEAQQIADDWCNLLKLSRDKNGRVSNLNYIADRLGLELTCALV
LGRRMGFLLPGAETETGEKLAEAVRQHFLGTRDTYFGFPFWKLFPTPAYKTLIKSEASIYELALELINSA
NESTKESAVFQSVIQAEIDEREKIAAIIDFISAGIHTMKNSLLFLLHLIGQDLQIQKKIIEDSTKSYSKA
CVTETFRLLPTANALGRILEEEMELGGYRLSAGTVVVCHFGIACRDERNFPDASKFKPERWLDDDKVQTA
TNSLFLLTPFGAGRRICPGKRFIEHILPLLLESTVNSFEIQAEEELELQFEFLVTPKGQMPMVFKDRV
CYP314A1 Aedes aegypti (yellow fever mosquito)
CYP314A1 Culex pipiens
CYP314A1 Culex_quinquefasciatus (southern house mosquito)
No accession number
Osamu Komagata
submitted to nomenclature committee 7/25/07
sequence 26
62% to CYP314A1 Anoph., 76% to 314A1 Aedes
Ortholog of shade shd gene a halloween gene in the Mito clan
CYP314A1 Bombyx mori (silkworm)
BP179750 BP179443 BAAB01069738.1 BAAB01063661.1
BAAB01099804.1 BAAB01099804.1 BAAB01122030.1 BAAB01134118.1
49% to 314A1 probable ortholog
See silkworm page for sequence
Ortholog of shade shd gene
CYP314A1 Manduca sexta (tobacco hornworm)
GenEMBL DQ372988
Rewitz,K.F., Rybczynski,R., Warren,J.T. and Gilbert,L.I.
Developmental expression of Manduca shade, the P450 mediating the
final step in molting hormone synthesis
Mol. Cell. Endocrinol. 247 (1-2), 166-174 (2006)
MSLPGVFLFSYYMKNVWSTPTPLLDWSCVPTLVLLVALVVVTVT
AVLTRAVDTKNPSRLPGPQPLPFLGTRWLFWSRYKMNKLHEAYEDMFRRYGPVFAETT
PGGATIVSIADREALDAVLKAPAKRPYRPPTEIVQVYRRSRPDRYASTGLVNEQGDKW
LHLRRHLTVELTSPNTMQGFMPELNSICEDFLDLLQSSRQANGTVHGFDQLTNRMGLE
SVCGLMLGSRLGFLERRMSGRAATLAAAVKTHFRAQRDSYYGAPLWKFAPTTLYKTFV
RSEETIHTVVSELMEEAKARTNGSAKDDGMQEIFLRILENPALDMRDKKAAVIDFITA
GIETLANSLVFLLYLLSGRPDWQHTIRSELPSCSRLSADDLSAAPSVRAAINEAFRLL
PTAPFLARLLDTPMTLGGHKLPAGTFVLAHTGAACRREENFWRAHEFLPERWSSSRQP
HAASLVAPFGRGRRMCPGKRFVELELHLLLAKIMQRWRVEFDGELDVQFDFLLSPKSP
VTLRLVEW
CYP314A1 Manduca sexta
No accession number
Yannick Pauchet
Submitted to nomenclature committee April 7, 2009
confidential
CYP314A1 Pediculus humanus humanus (body louse)
No accession number
Reed Johnson and Barry Pittendrigh
Submitted to nomenclature committee March 14, 2007
Ph314, 51% to 314A1 Apis
ortholog to shd shade
Pediculus genome site
CYP314A1 Nasonia vitripennis (jewel wasp)
See wasp page
CYP314A1 Daphnia magna (water flea)
GenEMBL AB257771
Yoshinori Ikenaka
Submitted to nomenclature committee 8/28/06
CYP family-1, full length sequence
41% to Cyp314a1 Drosophila, 40% to Apis CYP314A1
probable ortholog so the same subfamily name is used
CYP314A1 Daphnia pulex (water flea)
Confidential
Cyp315a1 Drosophila melanogaster
GenEMBL AC015141 229-2538 also AC008307 AC007725 AC007648
shadow sad gene
Cyp315a1 Drosophila pseudoobscura
See D. pseudoobscura page on this server for sequence
Obtained from UC Santa Cruz Browser
shadow sad gene
CYP315A1 Anopheles gambiae (malaria mosquito)
GenEMBL
Submitted by Christelle Abgrall, Hilary Ranson and Rene Feyereisen
See the paper: Evolution of supergene families associated with
insecticide resistance.
Ranson, H., Claudianos, C., Ortelli, F., Abgrall, C., Hemingway, J.
Sharakhova, M.V., Unger, M.F., Collins, F.H. and Feyereisen, R.
Science 298, 179-181 (2002)
Anopheles map name CYPs1x1
Note: in Drosophila Cyp315a1 is the shadow gene (sad), a C2 hydroxylase
in the ecdysteroid biosynthetic pathway from cholesterol
Ortholog of shadow sad gene
CYP315A1 Apis mellifera (honeybee)
WGS section genbank
Submitted by May Berenbaum 2/11/04
AmGroupUn.1189
38% to 315A1 Drosophila. Note this sequence is
named in the same subfamily as other CYP315s since
there seems to be only one orthologous sequence per
species and it does not make sense to name orthologs with
different subfamily names.
Ortholog of shadow sad gene
CYP315A1 Bombyx mori (silkworm)
AB167737 BAAB01119643.1
Niwa,R., Matsuda,T., Yoshiyama,T., Namiki,T., Mita,K., Fujimoto,Y.
and Kataoka,H.
CYP306A1, a cytochrome P450 enzyme, is essential for ecdysteroid
biosynthesis in the prothoracic glands of Bombyx and Drosophila
J. Biol. Chem. (2004) In press
probable ortholog
See silkworm page for sequence
Ortholog of shadow sad gene
CYP315A1 Manduca sexta (tobacco hornworm)
GenEMBL DQ357068
Rewitz,K.F., Rybczynski,R., Warren,J.T. and Gilbert,L.I.
Identification, characterization and developmental expression of
Halloween genes encoding P450 enzymes mediating ecdysone
biosynthesis in the tobacco hornworm, Manduca sexta
Insect Biochem. Mol. Biol. 36 (3), 188-199 (2006)
MHRMTRLLSKQQKLLIFQRNAASAECIRTDLTINEMPHPKSMPI
LGTKLEFFAAGGGKKLHEYIDNRHKQLGSIFCENLGGSADLVFISDPTLMKTLFLNLE
GKYPAHILPDPWILYEKLYGSKRGLFFMNGEEWLNNRRIMNKHLLKEDSEKWLNDPVK
ATIKSFINNWKTRAEQGNFIPDLETEFYRLSTDVIIAILLGSNSSIKTSKQYEMLLCM
FSESVKNIFQTTTKLYALPVTWCQRINLKVWRDFKECVDMSLFLAHKIVTEILNRRHE
NDGLIKRLCEDKMSDEDIIRIVADFVIAAGDTTAYTSLWTLLLMAKNKDYVNNELPMK
DINNIKHVVKEAMRLYPVAPFLTRILPKESILGPYKLNEGTPVIASIYTSGRDINNFS
RPEEFLPYRWDRNDPRKKELMNHNPSASLPFALGSRSCIGKKIAMLQITELMSQIVKN
FHLECLNKTPVNILTSQVLVPDKNIDIQVSLYDSSKLNKNECWL
CYP315A1 Manduca sexta
No accession number
Yannick Pauchet
Submitted to nomenclature committee April 7, 2009
confidential
CYP315A1 Tribolium castaneum (red flour beetle)
GenEMBL XP_970122
41% to 315A1 Drosophila
Ortholog of shadow sad gene
MLFRRVLAQLSTRPKSSLVSANTVLHFDEIPSPKGLPLVGTTLALIAHGSTPKLHDYVDKRHKQLGPIFK
EKLGPVSAVFVADPDEIRAVFDHEGKHPLHVLPDAWVAYNQMYGCPRGLFFMDGANWWYHRRIMNRLLLK
GDFRWIEGACECVSDKLIDSLMGESDYCGNLEATLYKWSLDVIVSILLGSGSYSQLCGELEPKVQKLAQV
THLVFQTSAKLALIPASFASKFKIPQWRRFVDSVDNALAQANALVDTLIEKKPHSDGLLPKLLAEQITLE
DIKRIIVDLVLAAGDTTAVAMEWMLYLVAKSPQIQEKLRRNPDFVKHVFKETLRLYPVAPFLTRILPEDA
ILGGYGVPKGTLVVMSIYTSGRDGRYFKNPGLFQPERWDRKDEFYSSEMKKASLPFAMGLRACVGRKVAE
TQLQMTLLKIVNKFEVELGNQREVEIVLKMVAVPSEPLLLKFKKL
CYP315A1 Aedes aegypti (yellow fever mosquito)
CYP315A1 Culex pipiens
CYP315A1 Nasonia vitripennis (jewel wasp)
See wasp page
CYP315A1 Daphnia pulex (water flea)
Confidential
Cyp316a1 Drosophila melanogaster
GenEMBL AC017388 35538-37158 also AC010113 AC010557 AC010112
Cyp316a1 Drosophila pseudoobscura
See D. pseudoobscura page on this server for sequence
Obtained from UC Santa Cruz Browser
Cyp317a1 Drosophila melanogaster
GenEMBL AC020477 126550-128106 no introns also AC009844 AL109349
Cyp317a1 Drosophila pseudoobscura
See D. pseudoobscura page on this server for sequence
Obtained from UC Santa Cruz Browser
CYP317A2 Lucilia cuprina (Australian blowfly)