P450s that have appeared since the 1993 P450 nomenclature update.
This is part A of the list covering CYP1 to CYP2
This includes references that were incomplete and duplications
of sequences that were already in the update. If a sequence
is assigned an accession number that was not in the old update
it is included in this list.
This list was last revised on Jan. 31, 2003.
Added all human genes and pseudogenes
Compiled by David R. Nelson
A new format is being designed to make the entries more useful, with links to
Genbank and Medline and access to the protein sequence. As time permits the
entries in the 1993 P450 Nomenclature Update will be added to make the
listing more comprehensive. For the time being, I will leave the old text
format in place below the newer table format, but eventually the text version
will be deleted. Any comments are welcome.
1A Subfamily
1B Subfamily
2A Subfamily
2B Subfamily
2C Subfamily
2D Subfamily
2E Subfamily
2F Subfamily
2G Subfamily
2H Subfamily
2J Subfamily
2K Subfamily
2L Subfamily
2M Subfamily
2N Subfamily
2P Subfamily
2Q Subfamily
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Cytochrome P450 Data CYP1 to CYP2 (Under Construction) |
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P450 gene |
Species |
Medline Entry |
Comment |
Protein Sequence |
Genbank Accession |
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CYP1A1 |
human |
none |
3' UTR |
D12525 D01198 |
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CYP1A1 |
human |
none |
3' UTR |
D12525 D01198 |
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CYP1A1 |
human |
none |
3' UTR |
D12525 D01198 |
|
|
|
CYP1A1 |
human |
none |
5' UTR |
D10855 D01150 |
|
|
|
CYP1A1 |
human |
none |
5' UTR |
D10855 D01150 |
|
|
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CYP1A1 |
Cavia cobaya |
none |
D11043 PIR S43414 |
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1A Subfamily
CYP1A1 human
GenEMBL D12525 D01198 (650bp)
Kawajiri,K., Watanabe,J., Gotoh,O., Tagashira,Y., and Sogawa,K.
Structure and drug inducibility of the human cytochrome P-450c
gene.
Eur. J. Biochem. 159, 219-225 (1986)
Kubota,M., Sogawa,K., Kaizu,Y., Sawaya,T., Watanabe,J.,
Kawajiri,K., Gotoh,O. and Fujii-Kuriyama,Y.
Xenobiotic responsive element in the 5'-upstream region
of the human P-450c gene.
J. Biochem. 110, 232-236 (1991)
Hayashi,S.-i., Watanabe,J., Nakachi,K. and Kawajiri,K.
Genetic linkage of lung cancer-associated MspI polymorphisms
with amino acid replacement in the heme binding region of
the human cytochrome P450IA1 gene.
J. Biochem. 110, 407-411 (1991)
CYP1A1 human
GenEMBL D10855 D01150 (4144bp)
Kawajiri,K., Watanabe,J., Gotoh,O., Tagashira,Y., and Sogawa,K.
Structure and drug inducibility of the human cytochrome P-450c
gene.
Eur. J. Biochem. 159, 219-225 (1986)
Kubota,M., Sogawa,K., Kaizu,Y., Sawaya,T., Watanabe,J.,
Kawajiri,K., Gotoh,O. and Fujii-Kuriyama,Y.
Xenobiotic responsive element in the 5'-upstream region
of the human P-450c gene.
J. Biochem. 110, 232-236 (1991)
Note: these refs are the same as the two earlier accession numbers.
CYP1A1 Macaca mulatta (rhesus monkey)
No accession number
Brian A. Carr, Merck & Co. Inc.
Submitted to nomenclature committee 4/22/2004
94% to CYP1A1 human, 73% to CYP1A2 human, ortholog of CYP1A1
CYP1A1 Cavia Cobaya (guinea pig)
GenEMBL D11043 (2674bp)
PIR S43414 (516 amino acids)
Ohgiya,S. Ishizaki,K. and Shinriki,N.
Molecular cloning of guinea pig CYP1A1: complete primary structure
and fast mobility of expressed protein on electrophoresis.
Biochim. Biophys. Acta 1216, 237-244 (1993)
CYP1A1 rat
GenEMBL I00732 (1800bp)
Oeda,K., Sakaki,T., Ohkawa,H., Yabusaki,Y., Murakami,H.,
Nakamura,K. and Shimizu,M.
Cytochrome P-450MC gene, expression plasmid carrying the said gene,
yeasts transformed with the said plasmid and a process for producing
cytochrome P-450MC by culturing the said transformant yeasts.
Patent: US 4766068-A 1 23-AUG-1988
CYP1A1 rat
PIR A93513 (524 amino acids)
Yabusaki, Y., Shimizu, M., Murakami, H., Nakamura, K., Oeda,
K. and Ohkawa, H.
Nucleotide sequence of a full-length cDNA coding for
3-methylcholanthrene-induced rat liver cytochrome P-450MC.
Nucleic Acids Res. 12, 2929-2938 (1984)
CYP1A1 rat
PIR S45716 (524 amino acids)
Omata, Y., Robinson, R.C., Gelboin, H.V., Pincus, M.R.,
Friedman, F.K.
Specificity of the cytochrome P-450 interaction with
cytochrome b(5).
FEBS Lett. 346, 241-245 (1994)
CYP1A1 rat
PIR D60822 (19 amino acids)
Amelizad, Z., Narbonne, J.F., Wolf, C.R., Robertson, L.W. and
Oesch, F.
Effect of nutritional imbalances on cytochrome P-450 isozymes
in rat liver.
Biochem. Pharmacol. 37, 3245-3249 (1988)
CYP1A1 hamster
GenEMBL D10913 (8700bp) Swiss Q00557 (524 amino acids)
Sagami,I., Ohmachi,T., Fujii,H., Kikuchi,H. and Watanabe,M.
Hamster cytochrome P-450 IA gene family, P-450IA1 and P-450IA2 in
lung and liver: cDNA cloning and sequence analysis
J. Biochem. 110, 641-647 (1991)
CYP1A1 hamster
PIR JS0746 (524 amino acids)
Ohgiya, S., Goda, T., Ishizaki, K., Morimoto, M., Sakamoto,T.,
Kamataki, T. and Shinriki, N.
unpublished (1992)
CYP1A1 rabbit
PIR A25143 (464 amino acids)
Okino, S.T., Quattrochi, L.C., Barnes, H.J., Osanto, S.,
Griffin, K.J., Johnson, E.F. and Tukey, R.H.
Cloning and characterization of cDNAs encoding 2,3,7,
8-tetrachlorodibenzo-p-dioxin-inducible rabbit mRNAs for
cytochrome P-450 isozymes 4 and 6.
Proc. Natl. Acad. Sci. U.S.A. 82, 5310-5314 (1985)
CYP1A1 Macaca irus (crab eating macaque monkey)
GenEMBL D17575 (2602bp)
Ohmachi,T., Sagami,I., Kikuchi,H., Fujii,H., Suzaki,Y., Fujiwara,T.
and Watanabe,M.
Molecular cloning and sequence analysis of cDNA encoding a
crab-eating monkey (Macaca irus) cytocrome P-450
unpublished (1993)
CYP1A1 Macaca fasicularis (crab eating macaque monkey)
Swiss P33616 (512 amino acids)
Komori, M. Kikuchi,O. Kitada,M. Kamataki T.
Molecular cloning of monkey 1A1 cDNA and expression in yeast.
Biochim. Biophys. Acta 1131, 23-29 (1992)
CYP1A1 Sus scrofa (pig)
GenEMBL AB052254
Misaki Kojima
Submitted to nomenclature committee Oct. 27, 2000
82% to human CYP1A1, 74% to human 1A2
CYP1A1 Ovis aries (sheep)
GenEMBL S79795 (2585bp)
Hazinski,T.A., Noisin,E., Hamon,I. and DeMatteo,A.
Sheep lung cytochrome P4501A1 (CYP1A1): cDNA cloning and
transcriptional regulation by oxygen tension
J. Clin. Invest. 96 (4), 2083-2089 (1995)
CYP1A1 Bos taurus (cow)
See cattle page for details
MFSVFGLPIPISATELLLASAVFCL
VFWVVRTWRPRVPQGLKSPPEPWGWPLLGHMLMLGKNPHVVLSQLSQRYGDVLQIRIG
CTPVLVLSGLDTVRQALVRQGDDFKGRPDLYSFTLITNGQSMTFNPDSGPVWAARRRL
AQNALKSFSTASDPASSSSCYLEEHVNKEAKYLLGKFQELMSGPGRFDPYRYIVVSVA
NVICAICFGRRYDHNDQEFLSLVNLSNEFGEITASGNPSDFIPVLRYLPNTALDLFKD
LNQRFYVFVQKIVKEHYKTFEKGHIRDITDSLIEHCQDKRLDENANIQLSDEKIINVV
IDLFGAGFDTVTTALSWSLLYLVTSPRVQKKIQEELDTVIGRARRPRLSDRPQLPYLE
AFILETFRHSSFVPFTIPHSTTRDSNLNGFYIPKGRCVFVNQWQINHDQKLWEDPSEF
RPERFLTADGTINKVLSEKVIIFGLGKRKCIGETIARLEVFLFLAILLHQVEFCVTPG
VKVDMTPVYGLTMKYARCEHFQAHMRS
CYP1A1 horse
No accession number
Heather Knych
Submitted to nomenclature committee Oct. 14, 2007
80% to CYP1A1 human, 70% to CYP1A2 human
CYP1A1 Macropus eugenii (tamar wallaby)
no accession number
Ross McKinnon
submitted to nomenclature committee 9/7/98
98 amino acid C-terminal fragment is 82% identical to macaque 1A1
CYP1A1 Balaenoptera acutorostrata (Minke whale)
no accession number
Ikuko Teramitsu, Yukio Yamamoto, and Shoichi Fujita
submitted to nomenclature committee 5/15/98
CYP1A1 Balaenoptera acutorostrata (Minke whale)
No accession number
Iwata Hisato
submitted to nomenclature committee 1/6/05
82% to CYP1A1 human, 74% to CYP1A2
CYP1A1 Pusa sibrica (Baikal seal)
No accession number
Iwata Hisato
submitted to nomenclature committee 1/6/05
82% to CYP1A1 human, 75% to CYP1A2
CYP1A1 Phocoenoides dalli (Dall's porpoise)
no accession number
Ikuko Teramitsu, Yukio Yamamoto, and Shoichi Fujita
submitted to nomenclature committee 5/15/98
CYP1A1 Eumetopias jubatus (Steller sea lion)
no accession number
Ikuko Teramitsu, Yukio Yamamoto, and Shoichi Fujita
clone #1
submitted to nomenclature committee 5/15/98
CYP1A1 Phoca largha (Spotted seal)
no accession number
Ikuko Teramitsu, Yukio Yamamoto, and Shoichi Fujita
submitted to nomenclature committee 5/15/98
CYP1A1 Phoca fasciata (Ribbon seal)
no accession number
Ikuko Teramitsu, Yukio Yamamoto, and Shoichi Fujita
submitted to nomenclature committee 6/29/99 revised 2/27/01
CYP1A1 Halichoerus grypus (grey seal, gray seal)
No accession number
Rachel Tilley
Submitted to nomenclature committee 3/19/2001
Name grey seal 1
CYP1A1 Phoca groenlandica (harp seal)
No accession number
Rachel Tilley
Submitted to nomenclature committee 3/19/2001
Name harp seal 1
Cyp1a1 mouse
GenEMBL K02588 (2619bp)
Kimura,S., Gonzalez,F.J. and Nebert,D.W.
The murine Ah locus.
J. Biol. Chem. 259, 10705-10713 (1984)
Cyp1a1 mouse
GenEMBL M10021 (8809bp)
PIR A24953 (30 amino acids)
Gonzalez,F.J., Mackenzie,P.I., Kimura,S. and Nebert,D.W.
Isolation and characterization of full-length mouse cDNA and
genomic clones of 3-methylcholanthrene-inducible cytochrome
P-1-450 and P-3-450
Gene 29, 281-292 (1984)
Cyp1a1 mouse
GenEMBL X01681 (6214bp)
Kimura,S., Gonzalez,F.J. and Nebert,D.W.
The murine Ah locus: Comparison of the complete cytochrome P1-450
and P3-450 cDNA nucleotide and amino acid sequences
J. Biol. Chem. 259, 10705-10713 (1984)
Cyp1a1 mouse
GenEMBL M11515 (8850bp)
Kimura,S. and Nebert,D.W.
Comparison of the mouse P-1-450 gene and flanking sequences from a
MOPC 41 plasmacytoma and normal liver.
DNA 4, 365-375 (1985)
Cyp1a1 mouse
GenEMBL M25623 (410bp)
Peterson,T.C., Gonzalez,F.J. and Nebert,D.W.
Methylation differences in the murine P-1-450 and P-3-450 genes in
wild-type and mutant hepatoma cell culture
Biochem. Pharmacol. 35, 2107-2114 (1986)
Cyp1a1 mouse
GenEMBL M33935 (474bp)
Jones,J.E. and Nebert,D.W.
Transcriptional start site in the mouse Cyp1a1 (cytochrome P-1-450) gene.
DNA 8, 527-534 (1989)
Cyp1a1 mouse
PIR C24406 (24 amino acids)
Cheng, K.C., Park, S.S., Krutzsch, H.C., Grantham, P.H.,
Gelboin, H.V. and Friedman, F.K.
Amino-terminal sequence and structure of monoclonal antibody
immunopurified cytochromes P-450.
Biochemistry 25, 2397-2402 (1986)
CYP1A1 Xenopus tropicalis (frog)
CYP1A2 human
GenEMBL M38504 (3149bp)
Jaiswal,A.K., Nebert,D.W., McBride,W.O. and Gonzalez,F.J.
Human P-3-450: cDNA and complete protein sequence, repetitive Alu
sequences in the 3' nontranslated region, and localization of gene
to chromosome 15
J. Exp. Pathol. 3, 1-17 (1987)
CYP1A2 human
GenEMBL U02993 (3293bp)
Quattrochi,L.C. and Tukey,R.H.
The human cytochrome Cyp1A2 gene contains regulatory elements
responsive to 3-methylcholanthrene
Mol. Pharmacol. 36, 66-71 (1989)
CYP1A2 human
PIR A25892 (515 amino acids)
Quattrochi, L.C., Pendurthi, U.R., Okino, S.T., Potenza, C. and
Tukey, R.H.
Human cytochrome P-450 4 mRNA and gene: part of a multigene
family that contains Alu sequences in its mRNA.
Proc. Natl. Acad. Sci. U.S.A. 83, 6731-6735 (1986)
CYP1A2 human
PIR A60881 (18 amino acids)
Wrighton, S.A., Campanile, C., Thomas, P.E., Maines, S.L.,
Watkins, P.B., Parker, G., Mendez-Picon, G., Haniu, M.,
Shively, J.E., Levin, W. and Guzelian, P.S.
Identification of a human liver cytochrome P-450 homologous
to the major isosafrole-inducible cytochrome P-450 in the rat.
Mol. Pharmacol. 29, 405-410 (1986)
CYP1A2 Macaca fascicularis (cynomolgus monkey)
GenEMBL D86474
Sakuma,T., Hieda,M., Igarashi,T., Ohgiya,S., Nagata,R., Nemoto,N.
and Kamataki,T.
Molecular cloning and functional analysis of cynomolgus monkey
CYP1A2
Biochem. Pharmacol. 56 (1), 131-139 (1998)
CYP1A2 Macaca fuscata (Japanese macaque)
GenEMBL AB185338 (hold till 7/22/2005)
Shizuo Narimatsu
Submitted to nomenclature committee 8/28/2004
99% identical to cynomolgus monkey CYP1A2
92.4% to human CYP1A2
CYP1A2 rabbit
PIR B27821 (516 amino acids)
Kagawa, N., Mihara, K., Sato, R.
Structural analysis of cloned cDNAs for polycyclic
hydrocarbon-inducible forms of rabbit liver microsomal
cytochrome P-450.
J. Biochem. 101, 1471-1479 (1987)
CYP1A2 dog
PIR A60463 (16 amino acids)
Ohta, K., Motoya, M., Komori, M., Miura, T., Kitada, M. and
Kamataki, T.
A novel form of cytochrome P-450 in beagle dogs. P-450-D3 is
a low spin form of cytochrome P-450 but with catalytic and
structural properties similar to P-450d.
Biochem. Pharmacol. 38, 91-96 (1989)
CYP1A2 Bos taurus (cow)
See cattle page for details
MALSQLSPFSAMELLLASAIFCLVFWVVRTWRPRVPQGLKSPPEPWGWPLLGHMLTLG
KNPHVVLSQLSQRYGDVLQIRIGCTPVLVLSGLDTVRQALVRQGDDFKGRPDLYSFTLVT
DGQSMTFNPDSGPVWAARRRLAQNALNTFSVASD
PSSSSSCYLEDHVSKEAEALLGKFQELMSGPGRFDPYGHVVASV
ANVIGAMCFGQHFPQSSKEMLSLVESSHDFVESASSGNPVDFFPILKYLPNPALQRFK
SFNQRFLQFVRKTVQEHYQDFDKNSIQDIIGALFKHSEDNSRASSRLISQEKTVNLVN
DLFAAGFDTITTAISWSLMYLVTNPKIQRKIQEELD
RVVGRARRPRLSDRPQLPYLES
FILETFRHSSFVPFTIPHSTTRDTTLNGFFIPKERCVFINQWQVNHDPKLWGDPSVFR
PERFLTSDGTTIDKTASEKVLLFGMGKRRCIGEVMARWEVFLFLAILLQRLEFSVPPG
VKVDLTPTYGLTMKHARCEHMQARLRFPIK
CYP1A2 Sus scrofa (miniature pig)
no accession number
Haitao Shang
Submitted to nomenclature committee May 23, 2007
86% to 1A2hum, 75% to 1A1hum
partial seq.
CYP1A2 Sus scrofa (miniature pig)
GenEMBL CB483208.1
KLWGDPSEFRPERFLTADGTAIHKTMSEEVILFGMGKRRCIGEVLAKWEVFLFLAILLQQ
LEFSVPP
CYP1A2 rat
PIR B24406 (25 amino acids)
Cheng, K.C., Park, S.S., Krutzsch, H.C., Grantham, P.H.,
Gelboin, H.V. and Friedman, F.K.
Amino-terminal sequence and structure of monoclonal antibody
immunopurified cytochromes P-450.
Biochemistry 25, 2397-2402 (1986)
CYP1A2 rat
GenEMBL X01031 (1106bp) PIR A44612 (367 amino acids)
Yabusaki, Y., Murakami, H., Nakamura, K., Nomura, N.,
Shimizu, M., Oeda, K. and Ohkawa, H.
Characterization of complementary DNA clones coding for two
forms of 3-methylcholanthrene-inducible rat liver
cytochrome P-450.
J. Biochem. 96, 793-804 (1984)
CYP1A2 rat
PIR S26822 (19 amino acids)
Botelho, L.H., Ryan, D.E., Yuan, P.M., Kutny, R., Shively,
J.E. and Levin, W.
Amino-terminal and carboxy-terminal sequence of hepatic
microsomal cytochrome P-450d, a unique hemoprotein from
rats treated with isosafrole.
Biochemistry 21, 1152-1155 (1982)
CYP1A2 rat
PIR D60822 (22 amino acids)
Amelizad, Z., Narbonne, J.F., Wolf, C.R., Robertson, L.W. and
Oesch, F.
Effect of nutritional imbalances on cytochrome P-450 isozymes
in rat liver.
Biochem. Pharmacol. 37, 3245-3249 (1988)
CYP1A2 rat
PIR A61400 (513 amino acids)
Woelfel, C.; Platt, K.L.; Dogra, S.; Glatt, H.; Waechter, F.;
Doehmer, J.
Stable expression of rat cytochrome P450IA2 cDNA and
hydroxylation of 17beta-estrodiol and 2-aminofluorene in
V79 Chinese hamster cells.
Mol. Carcinog. 4, 489-498 (1991)
CYP1A2 hamster
GenEMBL D10914 (9719bp)
Sagami,I., Ohmachi,T., Fujii,H., Kikuchi,H. and Watanabe,M.
Hamster cytochrome P-450 IA gene family, P-450IA1 and P-450IA2 in
lung and liver: cDNA cloning and sequence analysis
J. Biochem. 110, 641-647 (1991)
CYP1A2 Mesocricetus auratus (hamster)
GenEMBL M63787 M34446 (1868bp)
Lai,T.S. and Chiang, J.Y.L.
Cloning and characterization of two major 3-methylcholanthrene inducible hamster
liver cytochrome P-450s.
Arch. Biochem Biophys. 283, 429-439 (1990)
clone MC4
note: M34446 is incorrectly included in the GenBank entry
for CYP2A8 and CYP2A9. M34446 should only be in the CYP1A2 hamster entry.
CYP1A2 Cavia cobaya (guinea pig)
GenEMBL D50457 (1760bp)
Mori,T., Itoh,S., Ohgiya,S., Ishizaki,K. and Kamataki,T.
Effect of ascorbic acid on expression of several forms of
cytochrome P-450 of guinea pig
Unpublished (1995)
CYP1A2 Cavia porcellus (guinea pig)
GenEMBL U23501 (1757bp)
Black,V.H.
unpublished 1995
CYP1A2 chicken
GenEMBL M64537 (884bp)
Swiss Q01741 (258 amino acids)
Murti,J.R., Adiga,P.R. and Padmanaban,G.
Estradiol-17-Beta induces polyaromatic hydrocarbon-inducible
cytochrome p-450 in chicken liver
Biochem. Biophys. Res. Commun. 175, 928-935 (1991)
Note: previously called 1A2
CYP1A2 Eumetopias jubatus (Steller sea lion)
no accession number
Ikuko Teramitsu, Yukio Yamamoto, and Shoichi Fujita
clone #2
submitted to nomenclature committee 5/15/98
CYP1A2 Phoca fasciata (Ribbon seal)
no accession number
Ikuko Teramitsu, Yukio Yamamoto, and Shoichi Fujita
submitted to nomenclature committee 6/28/99 revised 2/27/01
CYP1A1/CYP1A2 chimera Phoca fasciata (Ribbon seal)
no accession number
Ikuko Teramitsu, Yukio Yamamoto, and Shoichi Fujita
submitted to nomenclature committee 6/28/99
on 2/27/01 the authors sent the following message
"... we believe that the production of the chimera
sequence could be the result of a PCR defect."
CYP1A2 Halichoerus grypus (grey seal, gray seal)
No accession number
Rachel Tilley
Submitted to nomenclature committee 3/19/2001
Name grey seal 2
CYP1A2 Phoca groenlandica (harp seal)
No accession number
Rachel Tilley
Submitted to nomenclature committee 3/19/2001
Name harp seal 2
Cyp1a2 mouse
GenEMBL K02589 (1893bp)
Kimura,S., Gonzalez,F.J. and Nebert,D.W.
The murine Ah locus.
J. Biol. Chem. 259, 10705-10713 (1984)
Cyp1a2 mouse
PIR A93512 (513 amino acids)
Kimura, S., Gonzalez, F.J. and Nebert, D.W.
Mouse cytochrome P-3-450: complete cDNA and amino acid
sequence.
Nucleic Acids Res. 12, 2917-2928 (1984)
Cyp1a2 mouse
GenEMBL X01682 (6715bp)
Kimura,S., Gonzalez,F.J. and Nebert,D.W.
The murine Ah locus: Comparison of the complete cytochrome P1-450
and P3-450 cDNA nucleotide and amino acid sequences
J. Biol. Chem. 259, 10705-10713 (1984)
Cyp1a2 mouse
GenEMBL M25624 (510bp)
Peterson,T.C., Gonzalez,F.J. and Nebert,D.W.
Methylation differences in the murine P-1-450 and P-3-450 genes in
wild-type and mutant hepatoma cell culture
Biochem. Pharmacol. 35, 2107-2114 (1986)
Cyp1a2 mouse
PIR B92495 (513 amino acids)
Gonzalez, F.J., Kimura, S. and Nebert, D.W.
J. Biol. Chem. 260, 11884-11889 (1985)
Erratum
Cyp1a2 mouse
GenEMBL M10022 (8865bp)
PIR B24953 (30 amino acids)
Gonzalez,F.J., Mackenzie,P.I., Kimura,S. and Nebert,D.W.
Isolation and characterization of full-length mouse cDNA and
genomic clones of 3-methylcholanthrene-inducible cytochrome
P-1-450 and P-3-450
Gene 29, 281-292 (1984)
Cyp1a2 mouse
PIR A45955 (42 amino acids) PIR B45955 (39 amino acids)
Peterson, T.C., Gonzalez, F.J. and Nebert, D.W.
Methylation differences in the murine P-1-450 and P-3-450
genes in wild-type and mutant hepatoma cell culture.
Biochem. Pharmacol. 35, 2107-2114 (1986)
Cyp1a2 mouse
PIR D24406 (25 amino acids) PIR E24406 (25 amino acids)
Cheng, K.C., Park, S.S., Krutzsch, H.C., Grantham, P.H.,
Gelboin, H.V. and Friedman, F.K.
Amino-terminal sequence and structure of monoclonal antibody
immunopurified cytochromes P-450.
Biochemistry 25, 2397-2402 (1986)
CYP1A2 Balaenoptera acutorostrata (Minke whale)
No accession number
Iwata Hisato
submitted to nomenclature committee 1/6/05
82% to CYP1A2 human, 69% to CYP1A1
CYP1A2 Pusa sibrica (Baikal seal)
No accession number
Iwata Hisato
submitted to nomenclature committee 1/6/05
80% to CYP1A1 human, 69% to CYP1A2
Fish Cytochrome P450s are undergoing a revision to their nomenclature. Initially
there appeared to be just one fish 1A gene per species, but that is not true as shown
by Amy Berndtson in trout. Until an adequate nomenclature can be devised, these fish
sequences are listed as CYP1A, without a number following the subfamily. This does
not affect the mammalian gene designations, though it may affect the chicken
sequences.
CYP1A1 Oncorhynchus mykiss (trout)
GenEMBL S69278 (5023bp)
Berndtson,A.K. and Chen,T.T.
Two unique CYP1 genes are expressed in response to
3-methylcholanthrene treatment in rainbow trout.
Arch. Biochem. Biophys. 310, 187-195 (1994)
Note: published as CYP1A2, but it is more similar to Heilmann's sequence
than Berndtson's 1A1 (97.9% identical).
CYP1A1 Oncorhynchus mykiss (trout)
GenEMBL U62797(1697bp)
Bailey,G., You,L. and Harttig,U.
Cloning, sequencing and functional expression of two trout CYP1A
cDNAs in yeast
unpublished (1997)
incorrectly called 1A2
CYP1A3 Oncorhynchus mykiss (trout)
GenEMBL U62796(2401bp)
Bailey,G., You,L. and Harttig,U.
Cloning, sequencing and functional expression of two trout CYP1A
cDNAs in yeast
unpublished (1997)
incorrectly called 1A1
CYP1A Oncorhynchus mykiss (trout)
GenEMBL AF015660
Bailey,G., You,L. and Harttig,U.
Cloning,sequencing and aflatoxin B1 metabolism by multiple rainbow
trout CYP1A cDNAs expressed in yeast
Unpublished
8 amino acid differences with U62797
CYP1A3 Oncorhynchus mykiss (trout)
GenEMBL S69277 (5524bp)
Berndtson,A.K. and Chen,T.T.
Two unique CYP1 genes are expressed in response to
3-methylcholanthrene treatment in rainbow trout.
Arch. Biochem. Biophys. 310, 187-195 (1994)
Note: published as CYP1A1. This sequence is 96.7% identical to
Heilmann's 1A1 sequence.
CYP1A1/CYP1A3 chimera Oncorhynchus mykiss (trout)
PIR A28789 (522 amino acids)
Heilmann, L.J., Sheen, Y.Y., Bigelow, S.W. and Nebert, D.W.
Trout P450IA1: cDNA and deduced protein sequence, expression
in liver, and evolutionary significance.
DNA 7, 379-387 (1988)
Published as CYP1A1
note: subsequent analysis has shown that the 5' end of this sequence
comes from the 1A3 gene and the switch over occurs between base 271
and base 435 with base 1 as the A of the ATG start codon.
CYP1A Pleuronectes platessa (plaice, a fish)
GenEMBL X73631 (2411bp) PIR S34184 (521 amino acids)
Leaver,M.J., Pirrit,L. and George,S.G.
Cytochrome P450 1A1 cDNA from plaice (Pleuronectes platessa)
Mol. Marine Biol. Biotechnol. 2, 338-345 (1993)
CYP1A Opsanus tau ( oyster toadfish)
GenEMBL U14161 (2352bp)
Morrison, H.G., Oleksiak, M.F., Cornell, N.W., Sogin,M.L. and
Stegeman, J.J.
Identification of Cytochrome P450 1A genes from two teleost fish, toadfish
(Opsanus tau) and scup (Stenotomus chrysops), and pyhlogenetic analysis
of CYP1A genes.
Biochem. J. 308, 97-104 (1995)
CYP1A Stenotomus chrysops (scup, a fish)
GenEMBL U14162 (1566bp)
Morrison, H.G., Oleksiak, M.F., Cornell, N.W., Sogin,M.L. and
Stegeman, J.J.
Identification of Cytochrome P450 1A genes from two teleost fish, toadfish
(Opsanus tau) and scup (Stenotomus chrysops), and pyhlogenetic analysis
of CYP1A genes.
Biochem. J. 308, 97-104 (1995)
CYP1A Chaetodon capistratus (four-eye butterfly fish)
GenEMBL U19855 (2552bp)
Vrolijk,N.H., Lin,C. and Chen,T.T.
Characterization and expression of a CYP1A gene from the tropical
teleost, Chaetodon capistratus.
Unpublished 1995
CYP1A Dicentrarchus labrax (european sea bass)
GenEMBL U78316(1563bp)
Stien,X., Amichot,M., Berge,J.-B. and Lafaurie,M.
Molecular cloning of a CYP1A cDNA from the teleost fish
Dicentrarchus labrax.
Unpublished (1995)
CYP1A1v2 Dicentrarchus labrax (european sea bass)
No accession number
Alessandra Salvetti
Submitted to nomenclature committee 11/26/99
94% identical to U78316 probably an allele
CYP1A Microgadus tomcod (Atlantic tomcod)
GenEMBL L41886 (2497bp) L41917
Roy,N.K., Konkle,B.A., Kreamer,G.-L., Grunwald,C. and Wirgin,I.I.
Characterization and prevalence of a polymorphism in the 3'
untranslated region of cytochrome P4501A1 in cancer-prone Atlantic tomcod
Arch. Biochem. Biophys. (1995) In press
probable frameshift detected by O. Gotoh. in the beginning of the sequence.
CYP1A Microgadus tomcod (Atlantic tomcod)
GenEMBL L41917 (6837bp)
Roy,N.K., Konkle,B. and Wirgin,I.I.
Functional characterization of Cytochrome P4501A1 regulatory
sequences in cancer-prone Atlantic tomcod.
Unpublished (1995)
CYP1A Pagrus major (wild red sea bream)
no accession number
Mizukami,M., Okauchi,M., Ariyoshi,T. and Kito,H.
The isolation and sequence of cDNA encoding a 3-methylcholanthrene-
inducible cytochrome P450 from wild red sea bream, Pagrus major.
Marine Biol. 120, 343-349 (1994)
CYP1A Sparus aurata (gilthead sea bream)
GenEMBL AF011223, AF005719
CYP1A Liza aurata
GenEMBL AF022433
Cousinou,M., Lopez-Barea,J. and Dorado,G.
CYP1A Liza saliens (leaping mullet)
GenEMBL AF072899
Alaattin Sen and Don Buhler
submitted to nomenclature committee
96% identical to Liza aurata
CYP1A Limanda limanda
GenEMBL AJ001724
Robertson,F.E., McPhail,M.E., Rankin,R., Stagg,R.M. and Craft,J.A.
CYP1A Platichthys flesus (European flounder)
GenEMBL AJ132353
Williams,T.D., Lee,J.S. and Chipman,J.K.
The cytochrome P450 1A gene (CYP1A) from European flounder
(Platichthys flesus), analysis of regulatory regions and
development of a dual luciferase reporter gene assay.
Unpublished
CYP1A1 Salmo salar (salmon)
No accession number
Christopher Rees Weiming Li
submitted to nomenclature committee Nov. 9, 2001
a second gene is being isolated so this is called 1A1
rather than just CYP1A. This does not imply orthology to the
mammalian 1A1, 1A2. The CYP1A gene duplications in fish and mammals
occurred independently.
CYP1A Anguilla anguilla (European eel)
GenEMBL AF420257
Mahata,S.C., Mitsuo,R., Aoki,J.-y., Kato,H. and Itakura,T.
Two forms of cytochrome P450 cDNA from 3-methylcholanthrene-treated
European eel Anguilla anguilla
Fish. Sci. 69 (3), 615-624 (2003)
98% identical to CYP1A9 from Japanese eel (clear ortholog)
note: Eels have two CYP1A sequences. This one is 80% identical to
Salmo salar CYP1A. CYP1A9 is 77% to the same Salmo CYP1A
Therefore, CYP1A9 is a recent duplication in eels that is diverging
Away from the parent sequence called CYP1A (no number after A).
Called CYPEuMC1
CYP1A Anguilla japonica (Japanese eel)
GenEMBL AB015638
Mitsuo,R., Itakura,T. and Sato,M.
Cloning and Sequencing of Cytochrome P450 1A Complementary DNA in
Eel (Anguilla japonica)
Mar. Biotechnol. 1 (4), 353-358 (1999)
98% identical to CYP1A9 from European eel (clear ortholog)
note: Eels have two CYP1A sequences. This one is 81% identical to
Salmo salar CYP1A. CYP1A9 is 78% to the same Salmo CYP1A
Therefore, CYP1A9 is a recent duplication in eels that is diverging
Away from the parent sequence called CYP1A (no number after A).
Called CYPJaMC1
CYP1A Danio rerio (zebrafish)
GenEMBL AY398333.1, AB078927.1
Gene is on CAAK02015935.1 (exon 1), CAAK02015934 (exons 2-6)
MALTILPILGPISVSESLVAIITICLVYLLMRLNRTKIPDGLQK
LPGPKPLPIIGNVLEIGNNPHLSLTAMSKCYGPVFQIQIGMRPVVVLSGNDVIRQALL
KQGEEFSGRPELYSTKFISDGKSLAFSTDQVGVWRARRKLALNALRTFSTVQGKSPKY
SCALEEHISNEGLYLVQRLHSVMKADGSFDPFRHIVVSVANVICGICFGRRHSHDDDE
LVRLVNMSDEFGKIVGSGNPADFIPFLRILPSTTMKKFLDINERFSKFMKRLVMEHYDTFDK (0)
DNIRDITDSLINHCEDRKLDENSNLQVSDEKIVGIVNDLFGA (1)
GFDTISTALSWAVVYLVHYPEVQERLQREL (1)
DEKIGKDRTPLLSDRANLPLLESFILEIFRHSSFLPFTIPHC (2)
TSKDTSLNGYFIPKDTCVFVNQWQVNHDP (2)
ELWKDPSSFIPDRFLTADGTELNKLEGEKVLVFGLGKRRCIGESIGRAEVFLFLAILL
QRLKFTGMPGEMLDMTPEYGLTMKHKRCLLRVTPQPVF
CYP1A Callorhinchus milii (elephant shark, Chondrichthyes)
Trace file 1573735839 78% to 1A zebrafish
1576735840 these two trace files are mate pairs
IRDITDSLIEHCQDKKMDENANIQVSDEKIINIVNDLFGA (1)
GFDTITTGLSWAVMYLVLYPDLQKRLQDEI (1)
DEKIGKDRSPRLSDRSRLPYTDAFILETFRYSSFLPFTIPHC (2)
TTKDTALNGYFIPKNTCVFVNQWQVNHDE (2)
CYP1A Petromyzon marinus (sea lamprey)
Trace files
1255373015 (DAVV exon +)
1386924597 (DAVV exon +)
1210995499 (DAVV exon +)
1437249679 (TTRD exon +)
1468852008 (TTRD exon +)
1442353648 (TTRD exon +)
1439550570 (ALWDE exon -) mate = 1442736929 = (TTRD exon +)
56% to 1A1 and 1A2 human, 61% to Bos 1A2
N-term part seems to be in a seq gap
DAVVGRQRRPSLNDRRQLPFTEAFILEVLRHSSVVPFTIPHS (2)
TTRDTVLQGFFIPKDTCIFINQWQVNHDS (2)
ALWDEPFAFRPERFLSEDQSSVDRTRAANLLSFGTGKRRCMGEAVARSELFLFLSILLHHL
RIRTADGQAPDMSAVYGLSLKHRTCLLLAESRS*
CYP1A4 Gallus gallus (chicken)
GenEMBL X99453(2098bp)
Gilday,D.J., Gannon,M., Yutzey,K., Bader,D. and Rifkind,A.
Molecular cloning and expression of two novel avian cytochrome P450
1A enzymes induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin.
J. Biol. Chem. 271, 33054-33059 (1996)
CYP1A4 Phalacrocorax carbo (Commmon Cormorant)
No accession number
Iwata Hisato
submitted to nomenclature committee 1/6/05
78% to CYP1A4 chicken, 72% to CYP1A5 chicken, 59% to CYP1A zebrafish
CYP1A5 Gallus gallus (chicken)
GenEMBL X99454(1845bp)
Gilday,D.J., Gannon,M., Yutzey,K., Bader,D. and Rifkind,A.
Molecular cloning and expression of two novel avian cytochrome P450
1A enzymes induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin.
J. Biol. Chem. 271, 33054-33059 (1996)
CYP1A5 Meleagris gallopavo (turkey)
No accession number
Roger Coulombe, Jr.
Submitted to nomenclature committee May 5, 2004
95% to chicken 1A5
CYP1A5 Phalacrocorax carbo (Commmon Cormorant)
No accession number
Iwata Hisato
submitted to nomenclature committee 1/6/05
78% to CYP1A5 chicken, 69% to CYP1A4 chicken, 58% to CYP1A zebrafish
CYP1A5 Corvus macrorhynchos (Jungle crow)
No accession number
Hisato Iwata
submitted to nomenclature committee 4/15/05
75% to 1A5 chicken 67% to 1A4 chicken
CYP1A6 Xenopus laevis (African clawed frog)
GenEMBL AB022087
Fujita,Y. and Ohi,H.
Xenopus laevis mRNA for cytochrome P450, cDNA clone MC1
unpublished(1999) In press
clone MC1
CYP1A7 Xenopus laevis (African clawed frog)
GenEMBL AB022088
Fujita,Y. and Ohi,H.
Xenopus laevis mRNA for cytochrome P450, cDNA clone MC2
unpublished(1999) In press
clone MC2
CYP1A8PX human
NT_008580.9
Pseudogene 43% identcal to 1A2 human
Renamed CYP1D1P orthologous to fish 1D1
NT_008580.9|Hs9_8737 chromosome 9
4822084 MILDLAVTPGEVTTSLIILVMVFVFVRALRSKGRKQVSPPGP*SFPIIENLLQLGDHPY 4822260
4822261 LTLMEMRKKYGDVFLLKLGMVPVLVVNGMEMVKQVLHKDGEHFAGRPNMHTFSFLAEGKS 4822440
4822441 LSFSVNYGESWKLHKKIASKAL*TFSNAEAKSSTCSCSLEEHVTEEISELVTVFVELTSK 4822620
4822621 NGSFDPRNAITCVVANIVCALCFGKR*DHSDEEFLRIVKTNDDLLKASSAANPADFIPCL 4822800
4822801 HYLPLKIINAPLEFYQALNGFIALHVQDHLATYGK 4822905 (0)
4824790 DHIRDITDALINVCHNKYAATKTDTLNDSEIISTVSDLFGA 4824912 (1)
4829424 GFETVSTCLCWSFLYLIHYPEIQARIQEEI 4829513 (1)
4829611 RPPRFEDRKILPYTEAFVSEVFRHASFLPFTIPHS 4829715 (2)
4832677 TTADTTLNGYFIPRKTCTFINMYQVNHDE 4832763 (2)
4835676 TIWDNHSLFRPDRFLNENRELNKSLVEKVLIFGMGIRKCLGEDVARNEIFIFITTVLQQFK 4835858
4835859 LKK*PRAKLDLTPTYGLVMRPKLYQLQAELHPSGSSSA* 4835975
CYP1A8PX ortholog Bos taurus (cow)
Renamed CYP1D1P orthologous to fish 1D1
See cattle page for details
MIFGMAVTSGEVTTSRIILVMVFVFVRELGNKGRKEVFPPGPWSLPIVENLLQLG
DHLYFTFMEMRKKYGDVFLIKLGMVPVLVVNGMEMVKEVLLRNGEHFAA*PNV
LTFSFLAQ*KSLTFS
NYGENWTLHKKIASNALRTFPKAETKSSTRSCLLEKHVIEEVSELVKV
FTELTSRSGSFEPRGAITCAMANVV
CTLCFGKRYDHSDEEFLRIVKTDHDLLKASSAANPADFIPYF*YLPLRIINAPQEFYHARNQ
FIALHIRDHLTT
CPQDHIQDITDALINACHNKYAVAKITILNDDEIISTVSDLVGAG
FEIISTCIYWSFLYLIYYPEIQVKIQEEI
DGNTGMKSPRFENRKILP
YTEAFINEIFRHTSFLPFTIPHC (2)
TTADTTLNGYFIPRKTCTFINMYQVNHDE (2)
TIWDNPNLLRPERFLNENRELNKNLIEKIFIFGMGIQKCL
REEVAQNEVFVFITTVLQQLTLKKCPVVKLDLTPTYGLVMKPKPYQLPAEPRSMGSSCS*
CYP1A8PX ortholog Xenopus tropicalis (frog)
This is not a pseudogene in frogs
It needs a new subfamily name, since it is
Separate from the CYP1A subfamily
See Xenopus page for seq
Renamed CYP1D1
CYP1A9 Anguilla anguilla (European eel)
GenEMBL AF420258
Mahata,S.C., Mitsuo,R., Aoki,J.-y., Kato,H. and Itakura,T.
Two forms of cytochrome P450 cDNA from 3-methylcholanthrene-treated
European eel Anguilla anguilla
Fish. Sci. 69 (3), 615-624 (2003)
98% identical to CYP1A9 from Japanese eel (clear ortholog)
note: Eels have two CYP1A sequences. CYP1A is 80% identical to
Salmo salar CYP1A. This seq is 77% to the same Salmo CYP1A
Therefore, this is a recent duplication in eels that is diverging
Away from the parent sequence called CYP1A (no number after A).
Called CYPEuMC2
CYP1A9 Anguilla japonica (Japanese eel)
GenEMBL AB020414
Mitsuo,R., Itakura,T. and Sato,M.
Cloning and Sequencing of Cytochrome P450 1A Complementary DNA in
Eel (Anguilla japonica)
Mar. Biotechnol. 1 (4), 353-358 (1999)
98% identical to CYP1A9 from European eel (clear ortholog)
note: Eels have two CYP1A sequences. CYP1A is 81% identical to
Salmo salar CYP1A. This seq is 78% to the same Salmo CYP1A
Therefore, this is a recent duplication in eels that is diverging
Away from the parent sequence called CYP1A (no number after A).
Called CYPJaMC2
1B Subfamily
CYP1B1 human
GenEMBL U03688 (5102bp)
Sutter,T.R., Tang,Y.M., Hayes,C.L., Wo,Y.-Y.P., Jabs,E.W.,
Li,X., Yin,H., Cody,C.W. and Greenlee,W.F.
Complete cDNA sequence of a human dioxin-inducible mRNA
identifies a new gene subfamily of cytochrome P450 that maps to
chromosome 2.
J. Biol. Chem. 269, 13092-13099 (1994)
*** Note The CYP1B1 gene has been linked to primary congenital glaucoma****
See April 97 Human Molecular Genetics
CYP1B1 human
GenEMBL U56438 (12177bp)
Tang,Y.M., Wo,Y.-Y.P., Stewart,J., Hawkins,A.L., Griffin,C.A.,
Sutter,T.R. and Greenlee,W.F.
Isolation and characterization of the human cytochrome P450 CYP1B1
gene.
J. Biol. Chem. 271, 28324-28330 (1996)
CYP1B1 Bos taurus (cow)
See cattle page for details
MATGLSPDDHLSPTLLSVQQTMLLLLLSVLAAVHVGQWLLRQRRRQPGSAPPGPFAWPLI
GNAASMGSAPHLLFARLARRYGDVFQIHLGSCRVVVLNGERAIRQALVHQSAAFADRPPF
ASFRLVSGGRSLAFGQYSESWKAQRRAAHSTMRAFSTRQPRGRRVLEGHVVGEVRELVEL
LVRRSAGGAFLDPRPLTLVAVANVMSALCFGCRYSHDDAEFLELLSHNEEFGRTVGAGSL
VDVLPWLQRFPNPVRTAFREFEQLNRNFSNFVLDKFLRHRESLRPGAAPRDMMDAFIHSA
GADSGDGGPRLDVDYVPATVTDIFGASQDTLSTALQWLLVLFTR (2)
YSEVQARVQAELDQVVGRHRLPTLEDQPRLPYVMAFLYEAMRFSSFVPVTIPHATTANAS
VLGYHIPKDTVVFVNQWSVNHDPVKWSNPEDFDPTRFLDKDGLINKDLTGSVMVFSVGKR
RCIGEEISKMQLFLFISILAHQCNFKANPDEPSKMDFNYGLTIKPKSFKINVTLRESMEL
LDSAVQKLQVEKECQ*
CYP1B1 rat
GenEMBL X83867 (2321bp)
Battacharyya,K.K., Brake,P.B., Eltom,S.E., Otto,S.A. and Jefcoate,C.R.
Identification of a rat adrenal cytochrome P450 active in polycyclic hydrocarbon
metabolism as a rat CYP1B1. Demonstration of a unique tissue-specific pattern of
hormonal and aryl; hydrocarbon receptor-linked regulation.
J. Biol. Chem. 270 11595-11602 (1995)
CYP1B1 rat
GenEMBL U09540(4964bp)
Nigel Walker
Walker,N.J., Gastel,J.A., Costa,L.T., Clark,G.C., Lucier,G.W. and
Sutter,T.R.
Rat CYP1B1: an adrenal cytochrome P450 that exhibits sex-dependent
expression in livers and kidneys of TCDD-treated animals.
Carcinogenesis 16 (6), 1319-1327 (1995)
Cyp1b1 mouse
GenEMBL U02479 (317bp)
Shen,Z., Wells,R., Liu,J. and Elkind,M.M.
Identification of a cytochrome P450 gene by reverse transcription-
PCR using degenerate primers containing inosine.
Proc. Natl. Acad. Sci. USA 90, 11483-11487 (1993)
Note: only 104 amino acids by PCR.
Cyp1b1 mouse
GenEMBL U03283 (5128bp)
Shen,Z., Liu,J., Wells,R.L. and Elkind,M.M.
cDNA cloning, sequence analysis, and induction by aryl hydrocarbons
of a murine cytochrome P450 gene, Cyp1b1.
DNA Cell Biol. 13, 763-769 (1994)
Cyp1b1 mouse
GenEMBL X78445 (2006bp)
Savas,U., Bhattacharyya,K.K., Christou,M., Alexander,D.L. and
Jefcoat,C.R.
Mouse cytochrome P450EF, representative of a new 1B subfamily of
cytochrome P450s. Cloning, sequence determination, and tissue
expression.
J. Biol. Chem. 269, 14905-14911 (1994)
CYP1B1 Xenopus tropicalis (frog)
See Xenopus page for seq
CYP1B1X Fundulus heteroclitus (killifish)
GenEMBL AF235140
Celine Godard, Maya Said and John Stegeman
Submitted to nomenclature committee Feb. 16, 2000
This seq is a CYP1C2 sequence not CYP1B1
CYP1B1 Platichthys flesus (European flounder)
GenEMBL AY304550
68% to 1B1 fugu
IKTIFXNFKKLNLEFGEFIRDKVIEHRKTIQSSTTRDMTDALIM
ALDKLGDKTELTGGKDYVSPTMGDIFGASQDTLSTALQWIVLILVKYPEMQLRVQQEV
DKVVERTRLPSIEDQLQL
CYP1B1 Danio rerio (zebrafish)
no accession number
66% to 1B1 fugu ctg26141 Length = 651601
4 exons
EST BQ419016
494367 MMDVLLALRDLLQLSTRSVLLSLMVCLMLMFRRRQLVPGPFSWPVIGNAAQLGNTP 494534
494535 HFYLSRMAQKYGDVFQIKLGSRNVVVLNGDAIKEALVKKATDFAGRPDFASFRFVSNGKS 494714
494715 MAFGNYTPWWKLHRKVAQSTVRNFSTANIQTKQTFEKHIVSEIGELIRLFLNKSREQQFF 494894
494895 QPHRYLVVSVANTMSAVCFGNRYAYDDAEFQQVVGRNDQFTKTVGAGSMVDVMPWMQYFP 495074
495075 NPIRTLFDQFKELNKEFCAFIELKVSEHRKTISPSHVRDMTDAFIVALDKGLSGGSGVSL 495254
495255 DKEFVPPTISDIF 495293
495379 GASQDTLSTALQWIILLLVR 495438
497442 YPEIQKRLQEDVDRVVDRSRLPTIADQPHLPYLMAFIYEVMRFTSFTPLTIPHS 497603
497604 TTKDTSINGYPIPKDTVIFVNQWSLNHDPTKWDQPEVFNPQRFLDEDGSLNKDLTTNVLI 497783
497784 FSLGKRRCIGEDVSKIQLFLFTSVLVHQCSFKAESTPNMDYEYGLTLKPKPFKVSVTARD 497963
497964 SSDLLDSLVGTSQTPTEKR 498020
CYP1B1 Danio rerio (zebrafish)
GenEMBL AF235139
Celine Godard, Maya Said and John Stegeman
Submitted to nomenclature committee Feb. 16, 2000
SQDTLSTALQWIILLLVRYPEIQKRLQEDVDRVVDRSRLPTIAD
QPHLPYLMAFIYEAMRFTSFTPLTIPHSTTKDTSINGYPIPKDTVIFVNQWSLNHDPT
KWDQPEVF
CYP1B1P Danio rerio (zebrafish)
No accession number (from trace index)
gnl|ti|30343474 zfishB-a1803b07.p1c Length = 630
probable 1B1 pseudogene zebrafish
IADQPHLPYMMAFIYEVMRFTSFTP
TTNVLIFSLGKRRCIGEDVSKIQLFLFTSVMVHQ*RIKAESTPNMGYVXXXXX
LKPKPFKVSVTARDSSDQLISLAGTSQTPTEK
CYP1B1 Cyprinus carpio (common carp)
GenEMBL AB048942
73% to 1B1 fugu
LSTALQWIILLLVRYPEVQKRLQEDVDKVADRSRLPTIADQPHL
PYVMAFIYEVMRFTSFVPVTIPYSTTTDTSINGYPIPKDTVIFV
CYP1B1 Cyprinus carpio (common carp)
No accession number
Itakura, T. and El-kady M.A.H.
Submitted to nomenclature committee 10/17/2003
Full length sequence 1 aa diff to fragment on AB048942
91% to CYP1B2 carp 64% to 1B1 fugu 53% to 1C1 fugu
clone name carp1B1a
CYP1B1 Stenella coeruleoalba (striped dolphin)
no accession number
Celine Godard, Maya Said and John Stegeman
submitted to nomenclature committee Nov. 20, 1998
PCR fragment 90% identical to human 1B1 I-helix to PERF motif region
CYP1B1 Pusa sibirica (Baikal seal)
No accession number
Iwata Hisato
submitted to nomenclature committee 1/6/05
84% to 1B1 human
CYP1B1 Pleuronectes platessa (plaice)
GenEMBL AJ249074
Michael Leaver
submitted to Nomenclature Committee 3/11/99
full length seq.
MFLQDPPAMDVTLEGIDPVTLRAVLLACVTLLFSLHLWRWLGGQ
PSVPGPPGPLAWPLIGNAAEMGKLPHLYLTRMAHKYGNVFQIKLGSRTVVVLNGDSIK
QALVKQGTDFAGRPDFASFKYIFDGDSLAFGPFTDWWKVHRRVAQSTVRTFSTGNADT
KKTFEHHVLCEFRELLQLFVGKTEQQRFFQPMTYLVVSTANIMSAVCFGKRYAYEDEE
FLQVVGRNDQFTQTVGAGSIVDVMPWLQYFPNPIRTIFDNFKKLNLEFGQFIRDKVIE
HRKTIQSSTTRDMTDALIVALDKLGDKSELTGGKDYVSPTMGDIFGASQDTLSTALQW
IVLILVKYPEMQLRIQQEVDKVVDRTRLPSIEDQLQLPYIMAFVYEVMRFTSFVPLTI
PHSTVTDTSIMGYTIPKNTVIFINQWSINHDPALWSHPETFDPQRFLDQNGALNKDLT
SSVLIFSLGKRRCIGEELSKMQLFLFTALIAHQCHISPDPARPPKLDYTYGLTLKPCA
FSIAVALRGHDMSLLDEATRSSAEEVKGEPSSDSQTKN
CYP1B1 Fugu rubripes (Takifugu rubripes) Japanese pufferfish
Scaffold_1553 complete gene Scaffold_11030 Scaffold_10662
54% TO 1B1 human 51% to 1B1 mouse
AL024920.1 AL015454.1 cosmid 077P23
80% to CYP1B from pleuronectes platessa
FC:C013F14aE4 LGU7740.y1 FC:C077P23aC12
AL015446.1 077P23 FC:C077P23aD8
2460 MKVIQEEVSPEAGALLLACATLLVSLQLWRWRRRRPGGCPPGPRAWPIIGNAAQLGHAPHL 2278
2277 YFTRMAQRFGNVFQIKLGSRTVVVLNGDAIKQALVRKGLEFAGRPDFTSFKYISNGHSL 2101
2100 AFGTVTDWWKSHRRVAQSTVRMFSTGNLQTKKTFERHLTCEVRELLHLFLGKTKELQYFQ 1921
1920 PMNYLVVSTANVISAVCFGKRYSYEDEEFQQVVGRNDQFTRTVGAGSIVDVMPWL 1756
1755 QYFPNPVKSIFDNFKRLNKEFSDFIRDKVTEHRKSIRPSSVRDMTDAFIVSLDKLSE 1585
1584 KTGVPLWKDYVIPTVGDVFGASQDTLSTALQWIFLVLVR 1468 (2)
294 YPDMQQRLQEEVDLVVGRQRLPCIEDQQQLPWVMAFIYEVMRFTSFVPLTIPHSTTTDTT 115
114 IMGYTIPKNTIIFINQWSINHDPTIWSHPET 13
FDPNRFLNPSGSLNKDLTSRMLIFSMGKRRCIGEELSKLHLFLFTALIGHQCHITDDPA
KPTTMDYNYGLTLKPRGFYVALTLRGDMRLLDEAASRPPAEEPGRGPLADP*
CYP1B1 Tetraodon nigroviridis (freshwater pufferfish)
No accession number
80% to CYP1B1 fugu missing first 50 aa and last 18 aa
FS_CONTIG_703_2 Length = 26665
69 NAAQLGKAPHLYFASRAERYGNVFQIRLGARSVVVLNGDAIRQALVKQGPEFAGRPDFAS 248
249 FGFISDGRSMAFGTATDWWKVHRRVAHSTVRMFSSGNAQTKKAFERHITSEVRELLRLFLRST 437
439 RAQRFFQPLAPLVVSTANVMSAVCFGKRYSYEDEEFQQVVGRNDQFTRTVGAGSVVDVMP 618
619 WLQYFPNPVKTIFDDFKRLNREFNSFIRDKVSEQ 720
722 RKTIQSSSVRDMTDALIASLDRLSAKTGVP 811
812 LWKEYVTPTVGDVFGASQDTLSTALQWIFLVLV 910
1486 RYPDVQQRLQKEVDQVVGRQRLPCLEDQQQLPWVMAFIYEVMRFTSFMPLTIPHSTTTDT 1665
1666 TIGGYSIPRNTVVFINQWSVNHDPAIWPQPETFDPDRFLNPNGSLNKDLTSSVLIFSLGK 1845
1846 RRCIGEELAKLHLFLFTALMGHQCRLASDPARPPSLDWNYGLTLKPHAFHIAVSLRGDMRLLDQ 2037
CYP1B1 Anguilla japonica (Japanese eel)
GenEMBL AB048940
73% to 1B1 fugu
LSTALQWIILVLVRFPDIQKQLREEVDKVVDSSRLPSIEDQPRL
PYVMAFLYEVMRFTSFIPVTIPHSTTTDTAIQGYRIPKDTVVFI
CYP1B1 Oreochromis niloticus (Nile tilapia)
GenEMBL AB048944
80% to 1B1 fugu
LSTALQWIILILVKYPEIQVRLQQEVDKVVDRSRVPAIEDQQQL
PYVMAFIYEVMRFTSFLPLTIPHSTTTDTSIMGYTVPKNTVIFI
CYP1B1 Callorhinchus milii (elephant shark, Chondrichthyes)
Trace files 1573810313 1573059473
57% to 1B1 zebrafish only 49% to 1C
MNAVRVLAGQFTQSMQPVLAVALVVLTLLQVCKWMQQPSEQCRRRPPGPFPWPII
GNATQIGKVPHISFSRMARRYGNVFQIKLGSRSVVVLNGEECIREALVRKAEQFSGRPDF
ASFNEVSGGRSLAFRSYCDRWKFHRRIAHSTVRAFSTNNPDTKKTFQRHVVGEVQQLSSR
RQ
CYP1B1 Petromyzon marinus (sea lamprey)
Trace files 1172235440, 1468167059, 1466822831, 1172788718,
1373603965, 1464676455
54% to 1B1 zebrafish, 48% to 1C2, 53% to CYP1B3 Petromyzon marinus
SSNVVEFALLVALEARRWLLLRRARSSRGPPGPFPWPILGNALQLGSAPHLAMCRMARRY
GDVFMMKLGGRPVLVLNGATAIRQALVKQGAD
FAGRPAFPSFSVVSDGNSMAFGGYSSLWKMHRCVAQST
LRHFSSSGNAEARADLERYV
VSEAGALVGIMLERSDGGRYFNPSRLFILAIANVMSALCFGRRYDYDNSEFREIV
SRNDKFGRTVGAGSLVDVMPWLLYFPNPVRTAYRDFVALNMEFNAFTRRKVEQHRADFKA
GGVPRDITDSLIAAVEVERPRSRSGEALSGRHVSGAVNDIFGASQDTLSTALMWLLMFLV
RFPRAQRRVQEEVD RVAGRHRLPCLEDRASLPYTEAFVFETLRYSSFVPV
TIPHSTTTDTVIAGYCVPKDTVVFVNQWSSNHDPERWRDPETFEPTRFL
DESGTRVDKDLASNVLIFSVGKRRCIGDDISKMQLLLFAAILAHQCSFEADPAQTMT
IDKSYGLTLKPMPFEVRARVRDHVLAECFADARRQL*
CYP1B3v1 Petromyzon marinus (sea lamprey)
Trace 1373790297 first exon 49% to 1B1 fugu, 50% to 1C1 zebrafish
1437356431 mate pair = 1438643165 = C=term of 1223244203 seq
1290968067 52% to Stenotomus chrysops P450 1C1
combined frags 49% to 1B1 zebrafish
45% to 1C2 zebrafish, 39% to 1A1 zebrafsih
1223244203, 1473037756, 1427240599, 1446950979 51% to 1B1
1438643165 = extreme C-term = mate pair of 1437356431
whole seq 51% to 1B1 human, 50% to 1B1 fugu, 49% to 1B1 zebrafish
MQSTLAILAVNPSRTPTSTASFTSTSTQLSIPSSHLPPPPPPPSIQPSSPAC
TLSQLPAHSPSAAASSPAVAAAPLHSLRTLPGPTPWPFVGNSLQLGPMPHLTFQRMASTY
GPLFRIRLGSRDVVVLNGDSLVREALVCRGSEFAGRPAFRSFSMVSGGHSV
AFGGYCELWRLHRRLAQSTLRAFSTGGTDARR ALDGHVMMEADELLRVMMA
SCRRSTAGSVDPAQALVVAVANVRSALCFRRRYWHED
AESSSSDRNERSGAAVGAGSVVDVMPW
LLRFPNPVRAAFDDIRRANEDLSEFVRDKVRQRRGAAAVVGPGTRSVRDMM
DALIAHVDGGAVAGGGAAEAAAGDGEGGEAAGGGRGGGGPRLGASHVEATLCDVFGASQD
TLSTGLLWLILLAVRHPEEQARVQGEVDRVVGRTRLPSAADRARMPRTEAFVCEVLRYSS
FVPVTIPHATTRDTRLAGYSIPRDTVVFVNQWSVNHDPGVFEEPHAFRPARF
LDAEGTALDRALARRVMIFSAGRRRCIGEELSRLELFLFTAVMLHQV
DFVAPPGHGPPGTEAVCGGLTLKPKPFSVALVPRGDPLGPGCAPQP*
CYP1B3v2 Petromyzon marinus (sea lamprey)
Trace files 1468808835, 1424613767 , 1489836465
allele of 1223244203? 4 aa diffs and one indel of 1aa
PVRAAFDDFRRANEDL
SEFVRDKVRQRRGAAAVVGPGTRSVRDMMDALISHVDGGAVAGGAAEAAAGDGEGGEAAGGERGGGGP
RLGASHVEATLCDVFGASQDTLSTGLLWLILLAVRHPEEQARVQGEVDRVVGRTRLPSAA
DRARMPRTEAFVCEVLRYSSFVPVTIPHATTRDTRLAGYSIPRDTVVFVNQWSVNHDPGV
FEEPHAFRPARFLDAEGTALDRALARRVMIFSAARFRCIGEELSRLELFL
CYP1B2X Stenotomus chrysops (scup, a fish)
no accession number
Celine Godard, Maya Said, and John Stegeman.
submitted to nomenclature committee full length 4/21/99
81% identical to scup 1B3
renamed CYP1C1
CYP1B3X Stenotomus chrysops (scup, a fish)
no accession number
Celine Godard, Maya Said, and John Stegeman.
submitted to nomenclature committee Aug. 26, 1998 full length 4/21/99
63% identical to human 1B1 over C-terminal PCR fragment
I-helix to heme
formerly 1B1, reaassigned to CYP1C2
Note: the CYP1B2 and 1B3 names from scup were never published.
It now appears that some fish like carp do have two CYP1B sequences, so the
CYP1B2 name is going to be used to indicate this fact. 10/20/2003
CYP1B2 Cyprinus carpio (common carp)
No accession number
Itakura, T. and El-kady M.A.H.
Submitted to nomenclature committee 10/17/2003
Full length sequence 3 aa diffs to fragment on AB048942
91% to CYP1B2 carp 64% to 1B1 fugu 53% to 1C1 fugu
clone name carp1B1b
CYP1C1 Stenotomus chrysops (scup, a fish)
no accession number
Celine Godard, Maya Said, and John Stegeman.
submitted to nomenclature committee full length 4/21/99
81% identical to scup 1C2
formerly 1B2, reaassigned after consultation with the submitters
and comparison to the Fugu genomic orthologs (see below)
CYP1C1 Danio rerio (zebrafish)
GenEMBL CAAK02055884.1 6714 bp gene seq (revised seq shown below)
contig NA9599 Length = 11279
78% to 1C1 73% to 1C2 fugu 53% to 1B1
Note: CYP1C probably arose by a retrotransposition of a 1B1 cDNA
Since 1C has no introns and it is more similar to 1B1 than 1A
MEAEFGLKSSSIMREWSGQVQPALIASFI
3411 ILFFLEACLWVRNLTFKKRLPGPFAWPLVGNAMQLGQMPHITFSKLAKKYGNVYQIRLGC 3232
3231 SDIVVLNGDAAIRKALVQHSTEFAGRPNFVSFQMISGGRSLTFTNYSKQWKTHRKVAQST 3052
3051 LRAFSMANSQTRKTFEQHVVGEAMDLVQKFLRLSADGRHFNPAHEATVAAANVICALCF 2872
2871 GKRYGHDDPEFRTLLGRVNKFGETVGAGSLVDVMPWLQS 2755
2753 FPNPVRSVYQNFKTINKGVFNYVKDKVLQHRDTYDRDVTRDMSDAIIGVIEHGKEST 2583
2582 LTKDFVESTVTDLIGAGQDTVSTAMQWMLLLLVKYPSIQSKLQEQIDKVVGRDRLPSIE 2406
2405 DRCNLAYLDAFIYETMRFTSFVP 2337
2337 VTIPHSTTSDVTIEGLHIPKDTVVFINQWSVNHDPQKWSDPHIFNPSRFLDENGALN 2167
2166 KDLTSSVMIFSTGKRRCIGEQIAKVEVFLFSAILLHQCKFERDPSQDLSMDCSYGLALKP 1987
1986 LHYTISAKLRGKLFGLVSPA* 1924
CYP1C1 Fugu rubripes
No accession number
Scaffold_3008b comp(8676-10253) no introns complete gene
86% to scup 1C1 75% to scup 1C2
10253 MALDTEFGVKSSSITREWSGQVQPALVASFLFLFCLEACLWVRNLRHKRRL
10100 PGPFAWPVVGNAMQLGQMPHITFAKLAKKYGNVYQIRLGCSNI 9972
9971 VVLNGDQAIHQALIEHSTEFAGRPNFVSFQMISGGRSLTFTNYSKQWKVHRKLAQSSLRA 9792
9791 FSSANKQTKIAFEQHVTAEANELVQAFLRYSTDGRYFDPAHEFTVAAANVMCALCFGKRY 9612
9611 GHDDHEFRCLLKKLNKFGETVGAGSLVDVMPWLQSFPNPVRSLYENFKSLNEEFFNFV 9438
9437 KNKVQEHRESFDPNVTRDMSDAMINVIEERKDGTLSKEFAEATITDLIGAGQDTVS 9270
9269 TVLQWIVLLLVKHPDKQAKLHELMDKVVGQDRLPTTEDRSSLAYLDAFIYETMRFTSFVP 9090
9089 VTIPHSTTSDVTIEGLRIPKDTVVFINQWSVNHDPLKWKDPHVFDPSRFLNENGDLNKDL 8910
8909 TSGVMIFSSGKRRCIGSQIAKVEVFLFAAILLHQCSFESDPSDPLTLDCSYGLTLKP 8739
LRCFVSAKPRGKLLGLVSPA* 8676
CYP1C1 Tetraodon nigroviridis (freshwater pufferfish)
No accession number
FS_CONTIG_2073_3 Length = 9880
87% to 1C1 70% to 1C2
5630 MALDTEFSVKSSGITREWSGQIQPALVASFLFLFCLEACLWVRNLRQKRRLPGPFAWPV 5806
5807 VGNAMQLGQMPHITFAKLAKKYGNVYQIRLGCSNIVVLNGDQAIXX 5938
5943 QALIQHSTEFAGRPNFVSFQMISGGRSLTFTSYSKQWKAHRKVAQSSLRAFSSANNQTKK 6122
6123 AFEQHVTAEANKLVQTFLHYSTDGKYFDPAHDFTIAAANVMCALCFGKRYGHDDQGVQVP 6302
6303 VNEVGQVWPRTVGAGSLVDVMPWLQSFPNPVRSVYENFKSLNEEFFSFVKNKVSEHRESF 6482
6483 DPNVTRDMSDAMINVIEGRKDSTLTKEFVEATVTDLIGAGQDTISTVMQWIILLLV 6650
6651 KYPDMQAKLHELVDKVVGQDRLPTVEDRSSLAYLDAFIYETMRFTSFVPVTIPHSTTSDV 6830
6831 TIEGLHIPKKDTVVFINQWSVNHDPLKWEG
6919 PHVLGPSRFLDDNGDLKKDLNKGVMIFSSGKRRCIGNQIAK 7041
7053 FLFTAILLHQCSFESNPSDPVTLDCSYGLTLKPLRCFVNAKPRGKLLGVVSPA 7211
CYP1C1 Anguilla japonica (Japanese eel)
No accession number
Itakura, T. and El-kady M.A.H.
Submitted to nomenclature committee 10/17/2003
Full length sequence 100% match to frag on AB048941
80% to 1C1 fugu 76% to 1C2 fugu 52% to 1B1 fugu
clone name Japanese eel 1C
CYP1C1 Anguilla japonica (Japanese eel)
GenEMBL AB048941
81% to 1C1 78% to 1C2 fugu
VSTLLQWILLLLVKYPHIQAKLQEQIDKVVGRDRLPCMEDKSSL
AYLDAFVYETMRFTSFVPVTIPHSTTSDVTIEGVHIPRDTVVFI
CYP1C1 Cyprinus carpio (common carp)
No accession number
Itakura, T. and El-kady M.A.H.
Submitted to nomenclature committee 10/17/2003
Full length sequence 2 aa diffs to frag on AB048943
77% to 1C1 fugu 73% to 1C2 fugu 50% to 1B1 fugu
clone name carp1C1a
CYP1C1 Cyprinus carpio (common carp)
GenEMBL AB048943
80% to 1C1 and 1C2 fugu
VSTVMQWILLLLVKYPSIQTKLQEQIDKVVGRGRLPSIEDKSNL
AYLDAFIYETMRYTSFVPVTIPHSTTSDVTIEGLHIPKDTVVFI
CYP1C1 Callorhinchus milii (elephant shark, Chondrichthyes)
Trace file 1576746999
57% to 1C2 tetraodon, 53% to 1B1 Pleuronectes, 49% to 1B1 fugu
This genomic fragment spans the location of 1B1s only
intron w/o an intron therefore this is probably 1C, an intronless gene
LVTVRTLYRDFKRLNQEFFGFVSGKVGQRRRTFVPGRTRDMSDAFIAVVDGAAAAGHGLS
GEHVEGTVNDVMGAGQDTTSTALGWVLFHLIRHPDVQARLQEEMDRAVGRGRLPGTGDRG
RLPYLQAFIHEVCRFTSFVPLTIPHATTSRVTLHGYDLPEDTVVFVNQWSVNHDGAKWKE
PETFEPGRFLDPDGSVNRALADSVMIFSAGKRRCLGDQLAKTQMFLFTAILIHQCAFEAN
PGDVLSLDCLYGLSLKPLPFKLRVRLRDTYRGVGRQREPPPPPTHTHTQKHSTGQGHTHR
DPSPTHTQRERDSQQDRDPTHHTPHRPLSTPVINVRN
CYP1C1 Petromyzon marinus (sea lamprey)
Trace files 1434207733, 1193330571,
1179606703, 1483258470, 1194048496, 1482130588, 1161783303, 1206198102
1193734487, 1468865778, 1293288933, 1162763713
53% to 1C2 Fugu 48% to 1B1 fugu (no intron so probably 1C)
MTAAESMEALPVVAAGGGAQLWDISHPPV
LFFLLSALLILLVTLEARKHGRSHQQQQKHSAPDPPGPLGFPIVGNSLQLGPM
PHLTLNAMAQRYGAVFRIHLGHEPVVVLTGEEI
IHEALVKRGAEFAGRPDFPSFALVSGGNSMSFKTYSELWRVHRRLAHSTLRAF
FTGTAATRRVFEGHVRLEAAELCAMLAEATSRAGGCGVDPSEPTVVAVANVISAVCFGKR
YEHDDAEFRGLLRNNERFSKTVGAGSVVDVMPWLMRFPNPVRSIFRDFEQMNNEFFAFVQ
RKVREHRDSYDPAATPRDMIDALIGHIDGGGGDSDDEGDAADGPSWRWRCARGAPEVGAA
YVDSTLTDVFGAG
QDTMSTSLMWFVLLCAKHPELQADMQRDIDRVVGRERLPRLDDRPQLACVDAFVCEMMRH
VSYVPFTIPHATTTDTELNGYRVAKGTVVFVNQWSVNHDPAIWRDPERFDPSRFL
DETGAALDRDLARRVMIFSAGKRRCIGYEMAKMQLFLFCSALLH
QLSISVPPGHVVSLEGVYGLSLKPKYLSVAFTPREQLLGGRPGEAEE*
CYP1C fragment Petromyzon marinus (sea lamprey)
Trace file 1483490875
frame3_ORF1 86% to CYP1C1 Petromyzon
TRRLAH
CTLRALFTGMATTRRVFEGHVRLEAAELCAMLHEQQNRAGGRGIESIERTVVAVANVISA
VCFGKRYEHEDAEFRGLLRNNERFSKTLGAGSVLEVIPWIMRFPNPARSIIREFEQMNNE
FFALMQRKVREHRDSYDPAATPRDMIDALIGHIDGGGGDSDDEGDAADG
QSWRWRCARGAPEVG
CYP1C2 Stenotomus chrysops (scup, a fish)
no accession number
Celine Godard, Maya Said, and John Stegeman.
submitted to nomenclature committee Aug. 26, 1998 full length 4/21/99
63% identical to human 1B1 over C-terminal PCR fragment
I-helix to heme
formerly 1B3, reaassigned after consultation with the submitters
and comparison to the Fugu genomic orthologs (see below)
CYP1C2 Danio rerio (zebrafish)
no accession number
contig NA2067 Length = 8014 EST CD758525
see zfish41356-444a08.p1c Zfish44625-3160d07.q1k
73% to 1C1 fugu and 74% to 1C2 fugu
MAQSDSEFSILKEWSGQIQPALIASFI
1098 ILCCLEACFWVRNITLKKKRLPGPFAWPLVGNAMQLGQMPHITFSKLAKKYGNVYQIRLG 1277
1278 SSDIVVLNGESAIRSALLQHSTEFAGRPNFVSFQYVSGGTSMTFASYSKQWKMHRKIAQS 1457
1458 TIRAFSSANSQTKKSFEKHIVAEAVDLVETFL 1553
KIQHFNPSHELTVAAANIICALCFRKRYGHDDLX (from EST CD758525)
(C-terminal inverted)
2818 IKNVLGNVNKFSETVGAGSLVDVMPWLQTFPNPIRSIFQSFKDLNSDFFSFVKGKVVEHRL 2636
2635 SYDPEVIRDMSDAFIGVMDHADEETGLTEAHTEGTVSDLIGAGLDTVSTALNWMLLL 2465
2464 LVKYPSIQSKLQEQIDKVVGRDRLPSIEDRCNLAYLDAFIYETMRFTSFVPVTIPHSTTS 2285
2284 DVTIEGLHIPKDTVVFINQWSVNHDPQKWSDPHIFNPSRFLDENGALDKDLTNSVMIFSI 2105
2104 GRRRCIGDQIAKVEVFLISAILIHQLTFESDPSQDLTLNCSYGLTLKPFDYKISAKPR 1931
1930 GSIVN* 1913
CYP1C2 Fugu rubripes
No accession number
Scaffold_3008a comp(5208-6770) no introns complete gene
83% to scup 1C2 78% to scup 1C1
6770 MEEDFGVKGSSSITREWSGHVQPALVAFFVFLFCVEACLWAKNLKRRL
6626 PGPFAWPVVGNAMQLGQMPHITFSKLAKKYGNVYQIRLGCSDI 6498
6497 VVLNGARVIRQALIEHSTEFAGRPNFVSFQNVSGGKSMAFTSYSKQWRMHRKIAQSTIRA 6318
6317 FSSANSQTKKVFEQQIVAEATELVEVFLKLGARGQHFNPAHELTVAAANVICALCFGRRY 6138
6137 GHDDQEFRDVLRRIDKFGQTVGAGSLVDVMPWLQSFPNPVRSMFRSFEALNREFFGF 5967
5966 VQLKVEQHRETFDPEVTRDMSDAIISVLEKSDGETALTKDYTEVTMADLIGAGLDTV 5796
5795 STALHWMLLLLVKHPELQSKLHQLIDRVVGRNRLPSIEDRSSLAYLDAFIYETMRFTSFV 5616
5615 PVTIPHSTTSDVTIEGLRIPKDTVVFINQWSVNQDPLMWKDPHVFDPSRFMDEEGSLDRD 5436
5435 LACNVMIFSAGKRRCIGDQIAKVEVFLFFAVLLHQCSFESSADEDLTLNCSYGLTLKPL 5259
5258 DFSITAKLRGKLLKSP* 5208
CYP1C2 Tetraodon nigroviridis (freshwater pufferfish)
No accession number
84% to CYP1C2 fugu 73% to CYP1C1 fugu
CNS_TRUECNSCONTIG_6508_2 Length = 4645
1369 MEEEFCVEGGSSSIREWSGHIQAALVAFFVFLFCLEARLWAKNL
1501 KRRLPGPFAWPVVGNAMQLGQMPHITFSKLAKKYGNVYQIRLGCSDIVVLNGDRVIRQAL 1680
1681 IQHSTEFAGRPNFVSFQTVSGGKGMTFSSYSKRWKMHRKIAQSTIRAFSSANSQTKENFE 1860
1861 QQIAAEATELVEVFLKLSARGQHFNPEHELTVAAANVICALCFGKRYGHDDAEFRELLHR 2040
2041 VNMFGQTVGAGSLVDVMPWLQSFPNPVRSMFKSFKTLNRQFFGFVQLKLKEHRETFDPKV 2220
2221 TRDMSDAIISVLDRSASEYGLTKDNAEGTVSDLIGAGLDTVSTALHWMLLLLVKHPQ 2391
2392 LQHKLQQLIDQVVGRNRLPSIGDRSSLAYLDAFIYETMRFTSFVPVTIPHSTTSDVTIEG 2571
2572 LRIPKDTVVFINQWSVNHDSLMWTDPHVFDPSRFLDEQGSLNRDLASNVMIFSAGKRRCI 2751
2752 GTQIAKAEIFLFLAILLHQCSFERSAGEEPSLDCSYGLTLKPLDYRITAKLRGKLLKSP 2928
CYP1C2 Fundulus heteroclitus (killifish)
GenEMBL AF235140
Celine Godard, Maya Said and John Stegeman
Submitted to nomenclature committee Feb. 16, 2000
Formerly named CYP1B1, but reassigned 10/21/2003
CYP1C2 Cyprinus carpio (common carp)
No accession number
Itakura, T. and El-kady M.A.H.
Submitted to nomenclature committee 10/17/2003
Full length sequence 5 aa diffs to frag on AB048943
73% to 1C2 fugu 72% to 1C1 fugu 51% to 1B1 fugu
clone name carp1C1b
CYP1D1P/CYP1A8PX human
NT_008580.9
Pseudogene 43% identcal to 1A2 human
Renamed CYP1D1P orthologous to fish 1D1
NT_008580.9|Hs9_8737 chromosome 9
4822084 MILDLAVTPGEVTTSLIILVMVFVFVRALRSKGRKQVSPPGP*SFPIIENLLQLGDHPY 4822260
4822261 LTLMEMRKKYGDVFLLKLGMVPVLVVNGMEMVKQVLHKDGEHFAGRPNMHTFSFLAEGKS 4822440
4822441 LSFSVNYGESWKLHKKIASKAL*TFSNAEAKSSTCSCSLEEHVTEEISELVTVFVELTSK 4822620
4822621 NGSFDPRNAITCVVANIVCALCFGKR*DHSDEEFLRIVKTNDDLLKASSAANPADFIPCL 4822800
4822801 HYLPLKIINAPLEFYQALNGFIALHVQDHLATYGK 4822905 (0)
4824790 DHIRDITDALINVCHNKYAATKTDTLNDSEIISTVSDLFGA 4824912 (1)
4829424 GFETVSTCLCWSFLYLIHYPEIQARIQEEI 4829513 (1)
4829611 RPPRFEDRKILPYTEAFVSEVFRHASFLPFTIPHS 4829715 (2)
4832677 TTADTTLNGYFIPRKTCTFINMYQVNHDE 4832763 (2)
4835676 TIWDNHSLFRPDRFLNENRELNKSLVEKVLIFGMGIRKCLGEDVARNEIFIFITTVLQQFK 4835858
4835859 LKK*PRAKLDLTPTYGLVMRPKLYQLQAELHPSGSSSA* 4835975
CYP1D1 Macaca mulatta (rhesus monkey)
chr15 from UCSC browser 81802360-81816347
92% to human 1D1P
MILNLAVTPGEVTTSLIILVMVFVFVRALRSKGRKQLSPPGPWSFPIIGNLLQLGEHPYL
TLMEMRKKYGDVFLLKLGMVPVLVVNGMEMVKQVLLKDGEHFAGRPNMHTFSFLAEGKSL
SFSVNYGESWKLHKKIASKALRTLSNAEAKSSTCSCLLEEHVTEEVSELVTVFVELSSKN
GGFDPRNAITCAVANVVCALCFGKRYDHSDEEFLKIVKTNDDLLKASSAANPADFIPCLR
YLPLQIINAPREFYRALNGFIALHVQDHLATYDK (0)
DHIRDITDALINVCHNKYAATKTDTLNDSEIISTVNDLFGA
GFETVSTCLYWSFLYLIHYPEIQAKIQEEI (1)
DGNIGLKPPRFEDRKILPYT
EAFISEVFRHASFLPFTIPHCNTADTTLNGYFIPRKTCTFINMYQVNHDETIWDNPSLFR
PDRFLNENRELNKSLVEKVLIFGMGIRKCLGEDVARNEIFIFITAVLQQLKLKKCPRAKL
DLTPTYGLVMRPKPYQLEAERRSSGSSSASILRLRGGFLTQFRKIDELNLLN*
CYP1D1P/CYP1A8PX ortholog Bos taurus (cow)
Renamed CYP1D1P orthologous to fish 1D1
See cattle page for details
MIFGMAVTSGEVTTSRIILVMVFVFVRELGNKGRKEVFPPGPWSLPIVENLLQLG
DHLYFTFMEMRKKYGDVFLIKLGMVPVLVVNGMEMVKEVLLRNGEHFAA*PNV
LTFSFLAQ*KSLTFS
NYGENWTLHKKIASNALRTFPKAETKSSTRSCLLEKHVIEEVSELVKV
FTELTSRSGSFEPRGAITCAMANVV
CTLCFGKRYDHSDEEFLRIVKTDHDLLKASSAANPADFIPYF*YLPLRIINAPQEFYHARNQ
FIALHIRDHLTT
CPQDHIQDITDALINACHNKYAVAKITILNDDEIISTVSDLVGAG
FEIISTCIYWSFLYLIYYPEIQVKIQEEI
DGNTGMKSPRFENRKILP
YTEAFINEIFRHTSFLPFTIPHC (2)
TTADTTLNGYFIPRKTCTFINMYQVNHDE (2)
TIWDNPNLLRPERFLNENRELNKNLIEKIFIFGMGIQKCL
REEVAQNEVFVFITTVLQQLTLKKCPVVKLDLTPTYGLVMKPKPYQLPAEPRSMGSSCS*
CYP1D1P dog
UCSC browser chr 1 87915406-87928215 (-) strand
57% to human 1D1P
VIAELISKNGNFGLRSVITCVVVVVNVICILCFSMRYD
HI*EEFLRIHKMNAHLLETSSEANPADFMPCFLYRPL*IINAYQEFYQAPN*FIALHDHLTTYDN
DHI*AIADALINACHNKYGTMEAATINDDEIISTMNGLFGA
GLETIAIFLFWGFLF
IIHFFQVKTWGWESVRFEHRKIIPYTEASIN*IFRYAPFLPLAIPHC (2)
STTEDTVQNGYFIPRKSCTFISMC*INHNQ
NIWDNPKLFRSQRFINENRE*KS*EQNVDIWNGTLEVSHRR**RNEICIFITSV
CYP1D1P Oryctolagus cuniculus (rabbit)
GenEMBL AAGW01268851.1
57% to human 1D1P, only 30% to 1A1
2347 VSVFVRALGSRNRKQVSTAGP*AFSNLFQLGAYPFLI**RGERNRDVFLFTFVVLP 2514
2515 VVVVNGMEMVKKTLLSDGKHFSGRPDMHTIAFLEEGKGLSSFVTHGES*KLYFQCVSNAL 2694
2695 CTFSKVEAK
FSTYSCLLEEHITEE
ASELMKVFVELTTKSGNFG 2825
2826 LRNAIPWHDQN
2857 IVGALCFGKRYDHNDGKSLSVVK
SNGLFKFPSKAKPQ
FIPQFHYLPLQIINIP*WL 3030
3031 YQALNQFTDLQVQGHLRMYDK 3093
CYP1D1P Sus scrofa
GenEMBL CT232614.1, CT282345.1
77% to human 1D1P only 32% to 1A1 human
376 VFVFVRALRNNGRKQVFPPGSCSFPIIGNLQLGGHPYLTFMEMRKKYGVVFFIKLGVMPV 555
556 LVVNGMEMVKQVLLKGGEHVAGRLHMHTFSFLAKGKSLTFLANYRESCKLCKKIASNAL* 735
736 TFSQEETKSPTCSCFLEEHVVEEVSELVKVFAELTSNSCSFDCRSAI 876
TVVANIVFALCFGKRYDHSDEEFLRIVKT
CYP1D1 Otolemur garnettii (small-eared galago)
GenEMBL WGS seq. AAQR01460136.1 N-terminal
6245 MISHLAITPREVTISLVILVIVFVFLRVLRSKGRKQVSPPGPLSFPIIGNLLQLGEHPYL 6066
6065 TFMEMRRQYGDIFLLRLGTVPVVVVNGVEMVKQVLLKDGEYFAGRPNMHTFSFLAEGKSL 5886
5885 TFSVNYGESWKLHKKIASNALRTFSKAEAKSSTCSCLLEEHVTEEASELVKVFVELTSKN 5706
5705 GSFNPRSAITCAVANVVCALCFGKRYDHGDEEFLRIVKTNDDLLKASSAANPADFIPCFR 5526
5525 YLPLRIINAPREFYQALNRFIALQVQDHLTTYDK 5424
CYP1D1 Myotis lucifugus (little brown bat)
GenEMBL WGS seq AAPE01629621
MULTIPLE FRAMESHIFTS, BUT NO STOPS, MAY BE SEQ ERRORS
13312 MILDKAITPEEVTTSLIILVIVFVFVRALMSKGRRQVSLPGPWSFPLIGNLLQLGDHPFL 13133
13132 TFTEMRKKYGDVFLIKLGMVPVVVVNGMEMVKHVLLKDGEHFAGRPNMHTFSFLAEGKSF 12953
12952 SFSVNYGESWKLHKKIASSALRTFSKAEAKSSTCSCLLEEQVIEEVSELVKVFAELTSKK 12773
12772 GSFEPRNAITCAVANVVCALCFGKRYDHSDEEFIRIVKTNDDLLKASSAANPADFIPCFR 12593
12592 YLPLRIINAPREFYRALNEFITLHVQDHLTTYDK (0) 12491
11217 DHMRDITDALINTCHKKICTTKXXXLNDDE II STVNDIXGA (1) 11131
10594 GFETVSTCLYWSFLYLIYYPEIQARIQEEI (1)
10415 DGNIGLKPPRFEDRKMLPYTEAFINEVFRHASFIPFTIPHC (2) 10293
8366 TTADTTLNGYFIPKNTCTFINMYQVNHDE 8280
5747 TIWDIQS VFSPERFLNENRELNKSLXX 5610
5601 KVLIFGMGIRKCLGEDVARNEVFLFITMVLQQLKLHKCPRAELDLTPTYGLAMKPKPYQL 5422
5421 QAEPRSADSAS* 5386
CYP1D1 Tupaia belangeri (northern tree shrew)
GenEMBL WGS seq. AAPY01014831.1 N-terminal
1294 MIFHLAVTPGEVTITLIILVVIFVFVKTLGNKGRKRLSPPGPWSFPIIGNLFQLGDHPYL 1115
1114 TFMEMRKKYGDVFMLRLGMVPVLVVNGMEMVKQVLLKDTEHFAGRPDMHSFSFLAEGKSL 935
934 SFSVNYGESWKLHKKIASNALRTFSKAEAKSSTCSCLLEEHVTEEVSELVKVFTESTSKN 755
754 GSFDPRNAITCAVANVVCALCFGKRYDHSDKEFLRIIKTNDDLLKASSAANPVDFIPCFR 575
574 YLPLRIINAPREFYRALNKFIALHVQDHITTYDK 473
CYP1D1 Sorex araneu (European shrew)
GenEMBL WGS seq. AALT01503634.1
12376 MIFNVAVNSGDLSTSLIVFVVVFVIVRALGSKGRKQGFPPGPRALPILGNLLQLGDYPYL 12197
12196 TFMEMRKKYGDVFLIRLGMVPVVVVNGMETVKQVLLKDGEKFAGRPKMHTFSFLAEGKSL 12017
12016 SFSVNYGESWKLQKKIASNSLRTFSKAEAKSSSCSCLLEEHVLEEVSELISIFEKLTSEN 11837
11836 GSFDPRNAITCAVANIVCALCFGKRYDHSDEEFLRIVKTNDDILKASSAANPADFIPCFR 11657
11656 YLPLPIVNGPRKFYRALNQFISLHVRDHYTTYDK 11555
9964 QDHIRDITDALISTCQNKYSSKKATLNDDEVISVVNDIFGA 9842
6041 GFETVSTCLYWSFLYLIQYPEIQVKVQEEI 5952
5868 IGLKSPTFEDRKILPYTEAFITEVFRHASFIPLTIPH 5758
2010 TVDTTLNGYFIPKKTCTFINMYQVNHDE 1927
CYP1D1 Echinops telfairi (small Madagascar hedgehog)
GenEMBL WGS seq. AAIY01323088.1
1272 MMFDSAAVPGEVTASLLVLVIVFVFIRARESQEGKKIPPPGPWSFPIIGNLLQLGAHPYL 1093
1092 TFMEMRKKYGDVFLIKLGVVPVLVVNGMEMVRRVLARDGEHFAGRPAMHTFSFLAEGKSF 913
912 SFSVNYGESWKLHKKIASNALRTFSKAEAKSSTCSCLLEEHVAEEVAVLVRAFAELTSTN 733
732 GSFEPRSVITCAVANVVCALCFGKRYEHSDEEFLKVVQTNDELLKASSAANPADFIPCFR 553
552 YLPLRIINAPREFYQALNQFITRHVQDHLTTYDK
CYP1D1 Loxodonta africana (African Elephant)
GenEMBL WGS seq. AAGU01360158.1
9163 MIFSLAVTPGEATTCLIVLVIVFVFVRALRNRDGKQVSLPGPWSFPIIGNLPQIGDHPYL 8984
8983 TFMEMRKKYGDVFLIRLGMVPVVVVNGMEMVKQVLLKDGEKFAGRPNMHTFSVLAEKKSL 8804
8803 SFSVNYGESWKLHKKIASNALRTFSKAEAKSSTCSCLLEEHVTEEVSELVKVFAELTSKN 8624
8623 GSFEPRSVITCSVANVVCALCFGKRYEHNDEEFLQIVKTNDELLKASSAANPADFIPCFR 8444
8443 YLPLGVINAPRKFYQALYQFIALHVQDHLTTYDKVRI 8333
6611 QDHIRDITDALINTCHNKHAATKTATLNDDEIINTVGDLFGA 6486
24XX GFETVSTCLYWSFLYLIRYPEIQAKIQEEI
DGNIGLKSPRFDDRKILPYTEAFVNEIFRHASFFPFTIPH 2139
CYP1D1 Monodelphis domestica (gray short-tailed opossum)
GenEMBL XM_001373076.1
72% to 1D1P human
not a pseudogene Built_from_Q9PTY7_and_others
405900 - 420186 bp (405.9 Kb) on chromosome fragment scaffold_15058
This transcript is located in sequence: contig_41044
MFVIETISKEVTISFLVLMIVFIFIRALGNRNKKHMSPPGPRPFPIIGNLLQLGDHPYLTFMEMKKKYG
DVFLIKLGMVPVVVVNGTEMVKKGLLKDGENFAGRPHMYTFSFFAEGKSLSFSVNYGESW
KLHKKIAMNALRNFSKAEAKSSTCSCVLEEHVTEEASELVKIFSKLSLKQGSFDPKSSIT
CAVANVVCALCFGKRYGHFDKEFLRIIKTNEEFLKASSAANPADFIPCFRYLPLRIIHAP
REFYCQLNHFIEQHVQDHITTFDKNHLRDITDALVSICRDKSATIKTATLSDNEIISTVS
DIFGAGFETVSGFLHWSFLYLIYYPEIQAKIHEEIDGIIGFKPPRFKDRKNLPYTEAFIN
EIFRHTTFVPFTIPHCTTKDTTLNGYFIPQKTCVFFNMYQVNHDETLWENPDSFQPERFL
NEKGEMNKNLVEKVLIFGMGIRKCLGEDVARNEVFIFIVSILQQLKLKKCPEVQLDLTPV
YGLVMKPKPYQLIVEPRFHVNSST*
CYP1D1 Ornithorhynchus anatinus (duckbill platypus)
GenEMBL AAPN01253410.1 16801-19436, AAPN01253411.1 386-472
AAPN01253413.1 1531-1812
74% to 1D1 opossum
MIPGELTTSLLMLVIVLISINVLRNRGQKPPSPPGPWALPVIGNLLQLGEHPYLSFIEMR
KKYGDVFLIKLGMVPVVVVNGMEPVKRVLFQDGENYAGRPNMHTFSFFANGKSLSFSTNY
GDSWKHHKKMAINALKSFSKAEAKSSTCSCLLEEHVCGEVSELVKIFTELTATQGNFDPR
GSLTCAVANVVCALCFGKRYEHTDEKFLKVIKINDDLLKASSAVNPADFIPCFRYLPLRV
VNAPREYYHMLNQFIMQHVQEHYVTYDE (0)
GYLRDITDALISICYDKNSTGKTPILPDDTIISTVNDIFGA (1)
GFDTVSTCLNWSFLYLINYPEIQTKIQAEI (1)
DGNIGLKPPRFEDRKNLPYTEAFINEIFRHTTFLPFTIPHC (2)
TTADTILNGYFIPQKTCVFVNIYQVNHDE (2)
TLWEKPDLFRPERFLNENGELNKGLVEKVLIFGLGIRKCLGEDVARNEIFIFITNVLQHL
KLEKCSGAQLDLTPVYGLSMKPKPYHIKAEPRF*
CYP1D2P Ornithorhynchus anatinus (duckbill platypus)
GenEMBL AAPN01177473.1
87% to CYP1D1 Ornithorhynchus
processed pseudogene no introns
DDTIISTANDIFGAGFDTVSTCLSRRFL*LINYREIQTKIQAEIDGNIGQEPPRFEDRKNLP
FTEGFINEIFRHTTFLPFTIPHCTTADISGYFIPQKTCIFVNKYQVNHDETLWENPDLFRPERFLNEN
CYP1D1/CYP1A8PX ortholog Xenopus tropicalis (frog)
This is not a pseudogene in frogs
It needs a new subfamily name, since it is
Separate from the CYP1A subfamily
See Xenopus page for seq
Renamed CYP1D1
CYP1D1 Danio rerio (zebrafish)
GenEMBL NM_001007310 5 introns
Note: CYP1C has no introns, 1B1 has 1 intron (not shared with 1D1)
CYP1A zebrafish has the same five introns
50% to CYP1A7 Xenopus, 49% to mouse Cyp1a1, 46% to 1A zebrafish
41% to 1C2 zebrafish, 36% to 1B1 zebrafish
89108 MNLENISHTATSEVTLILCAFALLLLALHGRRRAPGVPVPPGPRPWPIVG
NFLQMEEQVHLSLTNLRVQYGDVFQVKMGSLVVVVLSGYTTIKEALVRQGDA
FAGRPDLYTFSAVANGTSMTFSEKYGEAWVLHKKICKNALRTFSQTEPKDSNASCLLE
ERICVEAIDMVETLKAQGEEFGDSGIDPVQLLVTSVANVVCTLCFGKRYSHNDKEFLT
IVHINNEVLRLFAAGNLADFFPIFRYLPSPSLRKMVEFINRMNNFMERNIMEHLVNFDT (0) 89938
94917 NCIRDITDALIAMCEDRQEDKESAVLSNSQIVHSVIDIFGA (1) 95039
95618 GFDTIITGLQWSLLYLIKFPNIQDKIVQEI (1) 95707
98382 DNQVGMDRLPQFKDRPNMPYTEAFINEVFRHASYMPFTIPHC (2) 98507
98613 TTENITLNGYFIPKDTCVFINQYQVNHDI (2) 98700
101355 EIWDDPESFRPERFLTLSGHLNKSLTEKVMIFGMGIRRCLGDNIARLEM
FVFLTTLLHRLHIENVPGQELDLSSTFGLTMKPRPYRIKIIPRN* 101636
CYP1D1 Pimephales promelas (Cyprinid fish)
GenEMBL DT309726.1 EST testis
About 80% to zebrafish 1D1
69 MYLEEISRTTNVTSGLTLFLCAFALLLLALHGRRRGPGCSFPPGPKPWPLVGNLFQMGEQ 248
249 IHLSLTNLRVQYGDVFQVQMGSLVVVVLSGYSTIKEALVRKGEAFAGRPDLFTFSAVANG 428
429 TSMTFSEKYGEAWVLHKKICRNALRTFSQAEPRDSSASCLLEEHICTEAMEMVKALKEQG 608
609 DK 614
missing some sequence here
614 GNLADFFPIFRYLPSPSLRKMVQHIGRMNSFMECNIREHLITFDRNCIRDITDALIAMSE 793
794 DRQEDEETAMLSNSQIVHSVIDI 862
CYP1D1 Callorhinchus milii (elephant shark, Chondrichthyes)
GenEMBL CW874708.1 CW863449.1 GSS sequences
AAVX01473941.1 WGS
Trace archive files 1573350467 (exon 5) 1574214913 (exon 6)
1573943089 (exon 2)
About 67% to Gasterosteus aculeatus (stickleback) 1D1
PVEPITSTVANVICALCFGKRYEHNDKEFLNIVHTNHEVMRTFASGNVADVFPFFRYLPS
PSLKSMIKFVNRLNNFMIKSIQEHYTTFDK
GFDTIITGLQWCLLYLIQYPEFQTRIQQEI (1)
144 DEKVGQSRLPRFEDRTLLPFTEAFINEVFRHTTYMPFTIPHC (2) 19
TTASTTLNGYFIPKDTCVFINQYQVNHDE (2)
CYP1D1 Oryzias latipes
GenEMBL BAAF03028505.1 WGS seq
69% to zebrafish 1D1, only 48% to CYP1A
25653 MLSGTLPIA
25626 ESLSASLSSVTVVLFLIALGLMAIRVQKSRSSPFNVKDDSHLDLTAFPSPPGPTPWPIVG 25447
25446 NLFQMGNQMHLSLTLLRAKHGDVFK (0)
24429 LRLGSLPVVVLSGYNTIRQALVRQGEDFAGRPELFTFSAVADGTSMTFSEKFGPAWLLH 24253
24252 KKLCKNALRSFSQAAPRGSGATCLLEEHVCAEAAEMLEMIREQSAKVELDSEMTDGASKG 24073
24072 VDPVKPLVTSVANVVCALCFGKRYDHNDKEFLTIVNINNEVLKLFAAGNLADFFPVFRYF 23893
23892 PSLSLKELVQYIRRMNGFMERRIEEHMHTFDK (0) 23800
23189 NYIRDITDALIALCEDREKSKEMSLLSDTQIIHSVIDIFGA (1) 23067
22979 GFDTIIAGLQWSLLYLIKFPDVQRRIHQEI (1) 22890
20183 DEHIGSARMPNFSDKSKMPFTEAFIYEVFRHAAYVPFTIPHC (2) 20058
19961 TTRHTTLNGYFIPKDTCVFINQYQVNHDK (2) 19875
19791 DLWGDPEQFCPDRFLGHSGQLNKELTEKVLIFGMGKRRCLGDGFARLEMFVFLATLLHGL 19612
19611 RIENVPGQKLDLGTDFGLTMKPHPYKITVSSRFTEM* 19501
CYP1D1 Gasterosteus aculeatus (stickleback)
GenEMBL AANH01001861.1
77% to Oryzias 1D1
54662 MRVTFGIFPIKENTCASLSSVTVVLCLINLLLMALVCRKNHCHNSRLDHTKYPTPPGPT 54486
54485 PWPLVGNLLQMGDQIHLSLTRLRLQYGDVFK (0) 54393
54293 MRLGSLTVVVLSGHNTIRQALVRQGEAFAGRPDLFTFSAVANGTSMTFSEKYGPAWMLHK 54114
54113 KLCKNALRSFSRAEPRESGATCLLEEHVCAEAAEMVEVMYEQAAAEREMGHKVMGI 53946
53945 DPVVPVVTSVANVVCALCFGKRYDYNDKEFLTIVHINNEVLRIFAAGNMADFFPVFRYFP 53766
53765 SPSLRKMVQHIQRMNGFMERSIEEHINTFDK (0) 53673
53010 NYIRDITDALIALCEDREENQDTSLLSKSQIIHTVVDIFGA (1) 52888
52795 GFDTIIAGLQWSLLYLIKYPDIQDRIHQEI (1) 52706
51800 DDHIGIARLPMFSDKPKMPFTEAFMYEVFRHASYVPFTIPHC (2) 51675
51589 TTRNITLNGYFIPKDTCVFINQYQVNHD (2) 51506
51396 DLWGDPDRFRPARFLGSLGLLNKELTEKVLIFGVGKRRCLGDGLARLEMFVFLTTLLHRT 51217
51216 RIENVPGQQLDLSTDFGLTMKPRPYRITISSRF* 51115
CYP1E1 Ciona intestinalis (sea squirt)
JGI Ciona genome ver.2 gene model 131189
Clusters inside the vertebrate CYP1s on NJ trees
Closest to CYP1As, but only about 33% identical to CYP1As
Note: the Ciona genome is greatly diverged from the
Vertebrate line and seems to be undergoing rapid evolution
MMITAAILLDAGRSFAVPVAFTAVSVLTLYVCLRKRQGIPPGPTAWPLVGNL
FSMGRQSHLILESMRKTYGDVFSVYFGSTLVVVVNGKAVEECLSTHSAR (2)
YSMRPELHTAQYILEGKSFAFSHIAVSKHKRYRTLAVAVVKQLVNGGGEKTDVAV
KHGLQNGTRHSSIEERIFMEAACMCDKLLETSDSPDLKDEILKVITKEL (2)
LSEYELDEISRVVENLRNSNEAIMLVNFIPAVRMLWRNGLQKYIQLTQSLNR (2)
FFERCIRNRKAQLATVSNGHTEDNGVRLTNGVDCTVKFWQKLKNDPQYEESRVMKV (0)
VADLFGARVDTMTVALAWMIVYWSTYQAAQERAQKEIDHFVKNEKRLPR (2)
YSERNQLPYTMALIMEVERHCSFVPFTLPHAPAQDTMLNGYLIPKGTMMLISMRSINHDTAVWDSPAQFR (2)
PERFLLDQSGGFNSALAEQVMLFGAGRRRCAGEALGRMQIFLYSVLFLRKCTFRR
SDKDGHVLPESLAGISLIPQTMCVSISRREADGSKNTEP*
CYP1E1 Ciona savignyi (sea squirt)
Ortholog of C. intestinalis CYP1E1
Clusters inside the vertebrate CYP1s on NJ trees
Closest to CYP1As, but only about 33% identical to CYP1As
Note: the Ciona genome is greatly diverged from the
Vertebrate line and seems to be undergoing rapid evolution
75% identical to C. intestinalis CYP1E1
paired_scaffold_63
595236 SICLPITAFALSLIYLHRRKRDNLPPGPFAWPVLGNLLSLRSNSTAALEEIRRTYGDV 595063
595062 YSLYFGSRLVVVVNGKAVEECLSTRSAK 5949795
594724 RFSMRPELFTAQYVLGGKSFAFSHMDVETHRRYRKLAVGVVKELLVSTHERSQPTTMEEV 594545
594544 NRIPPQSIEDQIYAQAKRLCVGLFDIYASNSKSGQLDIRKEIMRRISFEM 594395
594161 LWEHELADLSELVEDLRNSNDATLILNFIPISRYLWKKGLRKYIKINQDLNK
592629 FFSRCFDRRNPHVANGSDCCKSEETCDVLSGIDCVLKLWQQLKDDPQFEENRVMKLVRKLFKCN 592438
591699 VGDLFGANVDTMTVALAWMIVYWSTYHQAQTRAQEEIDRFVETNFHLPRY 591550
591042 RYSDRSQLPFVMALIWEVARHCSFVPFALPHAPVEDTTLNGYLIPSGTVMMISMRSVNHDQTLWDS 590845
590844 PGEFR 590830
590562 PERFISSETGVFNKGLADRVMLFGGGRRRCAGEALARMQLFLFSVSILRSCTIRRVDHS 590386
590385 DVLPD 590371
CYP1F1 Ciona intestinalis (sea squirt)
JGI Ciona genome ver.2 gene model 136792
Clusters inside the vertebrate CYP1s on NJ trees
Closest to CYP1Bs and CYP1Cs, but only about 33% identical to CYP1Bs
Note: the Ciona genome is greatly diverged from the
Vertebrate line and seems to be undergoing rapid evolution
MLVQILTATFWTLIP
NSFGDLLIYAILVLTIVIYVKSLKRDKEWLALPGPIPW PLVGNA PFLGAEPHKKLLELSL
KYGPVYRLKMGGIKTVVLCNAEVVRSALIKQREAFSGRPKFSSYKAVS AGESVVFNDEET
LPP WRSH KSKIVRHMHKYTTSIRTRDKVTDLINTECMMMVTELDRISRSKCVNPENVIRM
ALANVMCAVCFGNRFEYDNE (0)
EFQKLLSMNTEFGAVIELGPIIDAMPWIK (0)
VIPKFKKAIADYLKINLQLDTWSRHR (2)
VDGVLKTFDNDDVTNVVASMTSEVLEKKSAGESREITESETKTIAALSADILGA
GQHTTSTTFFWVINLLLCFPKVLNKLTEEVRSKLGNRLPTLEDRTSLPYMDAVLTE
VLRFSSPLSSTIPHSTLKDVKLAGHTIKRGTMVIISQYAVNHDPQNWKNPENFDPERFLTK
NEGGEIIFNESLSEKVLAFSIGERKCPGSQLSRMLLFLATTLLVQVSDLSADLERPPT
AAAEYGLILRPKHLSIKLTLREHWQRRDSIRA*
CYP1F1 Ciona savignyi (sea squirt)
Ortholog of C. intestinalis CYP1F1
Clusters inside the vertebrate CYP1s on NJ trees
Closest to CYP1Bs and CYP1Cs, but only about 33% identical to CYP1Bs
Note: the Ciona genome is greatly diverged from the
Vertebrate line and seems to be undergoing rapid evolution
paired_scaffold_56 66% to C. intestinalis CYP1F1
957040 VLIYISMVSIVVIYVKSVKRNKEFMALPGPTPWPIVGNAPFLGKQPHKTLLQLSQK 956873
956872 YGPIYRLKMGSVEAVILCDLDVIRCALIKQREVFSGRPKFESYKAVSAGESVVFNDSESL 956693
956692 APWKSHKSKILRHLHKFATSVRTKEKVNNIITTECMLMLQCLHRRSQDGFVDPEDVIRMT 956513
956512 IANVMCAVCYGNRFEYENE 956456
950636 GQHTTSGTFFWVINILLFYPKVLQRITNEVRSKIGERIPTLEDQADLPYVEAFLTEV 950466
949639 VLRFASPLSSTIPHSTTKDTTLKGYKIKRNTMVIISQYSVNHDPKIWRNPEVFDPERFLTRDENTNLVFND 949427
949426 ALAEKVLSFSVGERKCPGSRMSQMVLFLATCLLVHTGTLYPNPDRPPS 949283
949282 PVDDAQYGLILRPEYISMKFLLDKKW 949205
CYP1F2 Ciona intestinalis (sea squirt)
JGI Ciona genome ver.2 gene model 143263
Clusters inside the vertebrate CYP1s on NJ trees
Closest to CYP1Bs and CYP1Cs, but only about 33% identical to CYP1Bs
Note: the Ciona genome is greatly diverged from the
Vertebrate line and seems to be undergoing rapid evolution
MDSLVFVLVDTVLVMKYQILLLLVIVYAIKLLAASQSRRLNIPGPYPWPVIGNVIEMGGQPQFSLTNMAK (?)
RYGPVYLMKLGTADVLVLNNYEVIKEALLRQRRIFGGRPIFDSFKKISQGLGVVFNSTMT
QGDEWMKLKMTIVKHVHRFVSSEETKGYVAHHVQMEAVELVRILTEKCRS
SPNEVIFPIEQINLAIANVVCAIMFGHRYQHGNK (0)
EFQDLISLNEQFGDVIGSGSQVDVIPWMK (0)
IFPKFRNALKVFDFLTNRLNNWMRLR (2)
TKEHRLTYKHGVIRDIVDSFIAESIDHPEQSALNDDVIMALTTDVFGA
GQDTMSTTMQWVFVYMMHFKECQRK
IHAELDSVIGPGELPHISDRRRLPYLEAVMHEIFRHSTFTSTTIPHVTTQDTVLDGHFIP
KGILVFINQFGANHDPNHWVDPDKFIPERFLDGKGNLISRPHDRYLLFSTGARKCPG
DELSRMLILHFMATMFALCEVSSDPQKPATL
DAVYNLSMRPKELRTIVRS
RNLPFLKNSVAQMSEADSHVLTVPGETTSFLTSRVESTVPDNQESQFSDNDFEKVDTKIP
KRKVFSRPTLTHDDINGNNVRKRGNLHQSAMYRIQLAT*
CYP1F2 Ciona savignyi (sea squirt)
Ortholog of C. intestinalis CYP1F2
Clusters inside the vertebrate CYP1s on NJ trees
Closest to CYP1Bs and CYP1Cs, but only about 33% identical to CYP1Bs
Note: the Ciona genome is greatly diverged from the
Vertebrate line and seems to be undergoing rapid evolution
paired_scaffold_142 77% to C. intestinalis CYP1F2
222183 FRRYGPIYLIKLGTADVLILNNYDVIKEALIRQRGVFSGRPVFESFKKISQ 222031
222030 GRGIVFNSSLTQGAAWQRMKMTIVKHLHRFIASPQTKGFVAGHVQKETVQLVHILSEKCR 221851
221850 SSTNQAIEPVENINLAVANVVCSIMFGHRYQHGNK 221746
219363 LHRTREHRQSYKHGVIRDLVDSFIAESIDKPGQLLNDDVIMALTTDVFGAGQDT 219202
219201 MSTTLQWIFVYMMRFKECQKK 219139
218667 IHAELDSVLKPGSLPQIKDRARLPYLEAVMHEIFRHSTFTTTTIPHVTTEDTVLRGYHLPKET 218479
218478 LIFINQYAANHDPEHWVEPDKFIPERFLDEKGNLISRPHDRYLLFSTGSRKCPGDELSRM 218299
218298 LILYLMANIFTLCEISPDPNQPTTLDAVYTLSMRPKNVKTVVRVR 218164
CYP1F3 Ciona intestinalis (sea squirt)
JGI Ciona genome ver.2 gene model 138492
Clusters inside the vertebrate CYP1s on NJ trees
Closest to CYP1Bs and CYP1Cs, but only about 33% identical to vert CYP1s
Note: the Ciona genome is greatly diverged from the
Vertebrate line and seems to be undergoing rapid evolution
LPYPRGLPIIGNIHQMGNFPHVKLTEWSKQFGDFYRIKMGRYDALVVNGHENIR (2)
NCLAKKSAAFAGRPPFETSKLIEEGLSISFSNYS (2)
PEWERQKQCTIKALKLYTSGSDKRSTMEETVSSHAKQLAEDLINSADQQ (0)
GLVGDLHDTVIYSTTSVSSTICFGRSFTRQDPELKEFLRNFQSFDKAMGASQIINFWPFLKYFPVLGKSFR (0)
NLKTYMDQYWNFTLSMLEQHWDTYVPNNMRDLADCLWAQSNQ (0)
NRQLTDQQRRIAYGASDAFGAGFDTISAMITWSIFYMAVFPEHQRK (0)
IREEIDRLETSMFSLRHHGDVCPYTQAWLYEVLRH
ISVSPLLVPHYTVKQVEVNGTMIPAGVVVLFNVAN (0)
ADRDTRVWENPEQFEPERFLARDPTTGGARVVASETSKI
LNWGAGKRRCPGAELSRHELFIYIANLVKLCYIE
QAVEGIEPAIPWPCTPGISTKPKAFRVKVTQR*
CYP1F3 Ciona savignyi (sea squirt)
Ortholog of C. intestinalis CYP1F3
Clusters inside the vertebrate CYP1s on NJ trees
Closest to CYP1Bs and CYP1Cs, but only about 33% identical to CYP1Bs
Note: the Ciona genome is greatly diverged from the
Vertebrate line and seems to be undergoing rapid evolution
paired_scaffold_3 56% to C. intestinalis CYP1F3
LPSPRGLPIIGNVHQLTTSPHVKLSEWAKEFGDLFRIKMGCFDTLVVTGYDNIR
(2) TALVKHSVAFAGRPPYETSKLFSNGLSLAFNNY
(2) SPAWEKQKRCTVKALKLYTAGPDLQKRNAMEDTASYQANLLVDQLLASVNK (0)
DAITNPDEIVHHSATNVISNICFGRSFSKNDPELQKFVSINRAFDRAMGSAQIVNFWPFLKSVPVLGRSYQ
NLKAHMDVFWDFVFPNLKEHWKTYNPSNIRDIADCLWYQSH
TSSKRDLQRRIASAASDIFGAGYDTTHKVVLWSLFYMAAFPQYQQKV
RDIFRVSEVKMY
TLRHHGDECPYVQAWIYEVLRHTSLAPILLPHYTTKEVTLNGVRIPAGVV
KKYHTIQAHKDPKIWKNPDEFDPGHFLEEDGSKLRSEAVHKLLSWGAGKRRCPGAELSRHE
IFVFVTTLVRRAYIGQAVDGVEPAFPWNTTGGISISPDPFRVKITER
CYP1F4 Ciona intestinalis (sea squirt)
JGI Ciona genome ver.2 gene model 132188
Clusters inside the vertebrate CYP1s on NJ trees
Closest to CYP1Bs and CYP1Cs, but only about 29% identical to vert CYP1s
Note: the Ciona genome is greatly diverged from the
Vertebrate line and seems to be undergoing rapid evolution
No ortholog is found in C. savignyi
MESVWVVIKWVKETMMSNSSFETIVAVATLLLLLMFVSENWNWLKIPGPI
PWPIIGNLGSLKGTKFLSIHEMYKIYGRIFRLKFGRVEAVVLCDVELIKE
ALLDRGRSLSGRPQFASYRLVSGCKSVVTNDPRCLREWVNY
KSTMVQTLCSISKNNEMKELMNERIGSVLVYMIQELEKGGDGQNFAEDIVTKTVANFLCT
VCYGGTYDFNSK (0)
EFNNLIEMSRHYTDNLSKSILRDMIPLAE (0)
ILPSVNKGRADFAKTSYHLHLWFLKR (2)
VEEVIQHFQPNKLNDLASVMVSDLTNDPTENISNITEKDRNSIAAIINDLVQ (1)
GYHSLYSMALWVVTYMIKYPEEVKKIENELNEVLDDYLPTLHDQESLPHTMAFINE (0)
VLRCRPSLPLAVPHSATEDTKLGGYDISKDTMVVASLYSANRDPKVWANPDQFDPSR
FLAKDDLGVTVLDETKVEQVFTFSLGDRKCPGEDIGRSFLFLTTAYLAHTCKLKPDPAK
PPTFQTKPGSITRPKDFGVQLNVKKCWLGVFKPDDNEE*
2A Subfamily
CYP2A1 rat
PIR C41425 (12 amino acids)
Imaoka, S., Kamataki, T. and Funae, Y.
Purification and characterization of six cytochromes P-450
from hepatic microsomes of immature female rats.
J. Biochem. 102, 843-851 (1987)
CYP2A1 rat
GenEMBl J02669
1 aa diff to genome seq (lower case)
82084958 MLDTGLLLVVILASLSVMLLVSLWQQKIRGRLPPGPTPLPFIGN
YLQLNTKDVYSSITQLSERYGPVFTIHLGPRRVVVLYGYDAVKEALVDQAEEFSGRGE
QATYNTLFKGYGVAFSSGERAKQLRRLSIATLRDFGVGKRGVEERILEEAGYLIKMLQ
GTCGAPIDPTIYLSKTVSNVISSIVFGERFDYEDTEFLSLLQMMGQMNRFAASPTGQL
YDMFHSVMKYLPGPQQQIIKVTQKLEDFMIEKVRQNHSTLDPNSPRNFIDSFLIRMQE
EKNGNSEFHMKNLVMTTLSLFFAGSETVSSTLRYGFLLLMKHPDVEAKVHEEIEQVIG
RNRQPQYEDHMKMPYTQAVINEIQRFSNLAPLGIPRRIIKNTTFRGFFLPKaTDVFPI
LGSLMTDPKFFPSPKDFDPQNFLDDKGQLKKNAAFLPFSTGKRFCLGDGLAKMELFLL
LTTILQNFRFKFPMKLEDINESPKPLGFTRIIPKYTMSFMPI
CYP2A1 rat
NP_036824 88% T0 2A2 chr1 (+) Cyp2a22 ortholog
82084958 MLDTGLLLVVILASLSVMLLVSLWQQKIRGRLPPGPTPLPFIGNYLQLNTKDVYSSITQ 82085134
82085434 LSERYGPVFTIHLGPRRVVVLYGYDAVKEALVDQAEEFSGRGEQATYNTLFKGY 82085595
82088031 GVAFSSGERAKQLRRLSIATLRDFGVGKRGVEERILEEAGYLIKMLQGTC 82088180
82088398 GAPIDPTIYLSKTVSNVISSIVFGERFDYEDTEFLSLLQMMGQMNRFAASPTG 82088556
82089778 QLYDMFHSVMKYLPGPQQQIIKVTQKLEDFMIEKVRQNHSTLDPNSPRNFIDSFLIRMQE 82089957
82093158 EKNGNSEFHMKNLVMTTLSLFFAGSETVSSTLRYGFLLLMKHPDVE 82093295
82093737 AKVHEEIEQVIGRNRQPQYEDHMKMPYTQAVINEIQRFSNLAPLGIPRRIIKNTTFRGFFLPK 82093925
82094440 GTDVFPILGSLMTDPKFFPSPKDFDPQNFLDDKGQLKKNAAFLPFST 82094580
82098022 GKRFCLGDGLAKMELFLLLTTILQNFRFKFPMKLEDINESPKPLGFTRIIPKYTMSFMPI 82098201
CYP2A1-de2b rat
exon 2 pseudogene Chr1 (-) only 240 bp from CYP2A1 start Met
frag e in fig below
82084718 YNAVKEALVDQAEGFSGQGEQA 82084653
rat, mouse and human 2ABFGST clusters
CYP2A2 rat
PIR S26821 (27 amino acids)
Matsumoto, T., Emi, Y., Kawabata, S. and Omura, T.
Purification and characterization of three male-specific and
one female-specific forms of cytochrome P-450 from rat
liver microsomes.
J. Biochem. 100, 1359-1371 (1986)
CYP2A2 rat
J04187 Cyp2a12 ortholog
82117349 MLDTGLLLVVILASLSVMFLVSLWQQKIRERLPPGPTPLPFIGNYLQLNMKDVYSSITQ 82117525
82117991 LSERYGPVFTIHLGPRRIVVLYGYDAVKEALVDQAEEFSGRGELPTFNILFKGY 82118152
82123228 GFSLSNVEQAKRIRRFTIATLRDFGVGKRDVQECILEEAGYLIKTLQGTC 82123377
82123595 GAPIDPSIYLSKTVSNVINSIVFGNRFDYEDKEFLSLLEMIDEMNIFAASATG 82123753
82124978 QLYDMFHSVMKYLPGPQQQIIKVTQKLEDFMIEKVRQNHSTLDPNSPRNFIDSFLIRMQE 82125157
82139054 EKYVNSEFHMNNLVMSSLGLLFAGTGSVSSTLYHGFLLLMKHPDVE 82139191
82139607 AKVHEEIERVIGRNRQPQYEDHMKMPYTQAVINEIQRFSNLAPLGIPRRIIKNTTFRGFFLPK 82139795
82140311 GTDVFPIIGSLMTEPKFFPNHKDFNPQHFLDDKGQLKKNAAFLPFSI 82140451
82141451 GKRFCLGDSLAKMELFLLLTTILQNFRFKFPMNLEDINEYPSPIGFTRIIPNYTMSFMPI 82141630
CYP2A2-de2b rat
exon 2 pseudogene Chr1 (-) frag f in fig below
82115528 LKPHWVVVLYEWDAVKEALGDQAEELSG*GEQANL 82115445
rat, mouse and human 2ABFGST clusters
CYP2A3 rat
J02852 NM_012542 exon 4 in a seq gap in genome seq chr1 (+)
mouse Cyp2a5 ortholog
82023007 MLASGLLLVASVAFLSVLVLMSVWKQRKLSGKLPPGPTPLPFIGNYLQLNTEKMYSSLMK 82023186
82023453 ISQRYGPVFTIHLGPRRVVVLCGQEAVKEALVDQAEEFSGRGEQATFDWLFKGY 82023614
82024296 GVAFSSGERAKQLRRFSIATLRDFGVGKRGIEERIQEEAGFLIESFRKTN 82024445
GALIDPTFYLSRTVSNVISSIVFGDRFDYEDKEFLSLLRMMLGSFQFTATSTG
82026488 QLYEMFSSVMKHLPGPQQQAFKELQGLEDFITKKVEQNQRTLDPNSPRDFIDSFLIRMLE 82026667
82028068 EKKNPNTEFYMKNLVLTTLNLFFAGTETVSTTLRYGFLLLMKHPDIE 82028208
82028659 AKVHEEIDRVIGRNRQAKYEDRMKMPYTEAVIHEIQRFADMIPMGLARRVTKDTKFREFLLPK 82028847
82029417 GTEVFPMLGSVLKDPKFFSNPNDFNPKHFLDDKGQFKKSDAFVPFSI 82029557
82030741 GKRYCFGEGLARMELFLFLTNIMQNFCFKSPQAPQDIDVSPRLVGFATIPPNYTMSFLSR 82030920
CYP2A3-de1b rat
exon 1 pseudogene Chr1 (+)frag d in fig below
82052140 MLGSRLLLVAVLSCLCVMVFMPVWQQQYRDTIPPG 82052244
rat, mouse and human 2ABFGST clusters
Cyp2a4 mouse
GenEMBL J04631 (multiple genomic fragments)
PIR A30499 (494 amino acids) PIR A33531 (494 amino acids)
Lindberg,R., Burkhart,B., Ichikawa,T. and Negishi,M.
The structure and characterization of type I P-450-15-alpha gene as
major steroid 15-alpha-hydroxylase and its comparison with type II
P-450-15-alpha gene
J. Biol. Chem. 264, 6465-6471 (1989)
Cyp2a4 mouse
PIR S16067 (494 amino acids)
Squires, E.J. and Negishi, M.
Reciprocal regulation of sex-dependent expression of
testosterone 15-alpha-hydroxylase (P-450-15-alpha) in liver
and kidney of male mice by androgen. Evidence for a single gene.
J. Biol. Chem. 263, 4166-4171 (1987)
Note: 2a-4 and 2a-5 differ at 11 positions. This sequence is 2a-4 like at
9/11 positions.
Cyp2a4-de7b mouse
GenEMBL AC087157.1 + strand
w in Figure 2B Nelson et al. Pharmacogenetics 14, 1-18 (2004)
detritus exon 7 between Cyp2a4 and Cyp2b9
37037 AKIHEEINQVIGTHRTPRVDDRAKMP 37114
37114 YTDAVIHEIQRLTDIVPLGIPHNVT 37188
37190 RDTHFRGY 37213
Cyp2a5 mouse
GenEMBL J04631 (multiple genomic fragments)
PIR B30499 (494 amino acids) PIR B33531 (494 amino acids)
Lindberg,R., Burkhart,B., Ichikawa,T. and Negishi,M.
The structure and characterization of type I P-450-15-alpha gene as
major steroid 15-alpha-hydroxylase and its comparison with type II
P-450-15-alpha gene
J. Biol. Chem. 264, 6465-6471 (1989)
Cyp2a5 mouse
PIR S16068 (494 amino acids)
Squires, E.J. and Negishi, M.
Reciprocal regulation of sex-dependent expression of
testosterone 15-alpha-hydroxylase (P-450-15-alpha) in liver
and kidney of male mice by androgen. Evidence for a single gene.
J. Biol. Chem. 263, 4166-4171 (1987)
Note: 2a-4 and 2a-5 differ at 11 positions. This sequence is 2a-4 like at
5/11 positions, and 2a-5 like at 6/11 positions
Cyp2a4 or 5 mouse
PIR S03979 (21 amino acids)
Lang, M.A., Juvonen, R., Jaervinen, P., Honkakoski, P. and
Raunio, H.
Mouse liver P450Coh: genetic regulation of the
pyrazole-inducible enzyme and comparison with other P450
isoenzymes.
Arch. Biochem. Biophys. 271, 139-148 (1989)
CYP2A6 human
PIR S17220 (20 amino acids)
Maurice, M., Emiliani, S., Dalet-Beluche, I., Derancourt, J.
and Lange, R.
Isolation and characterization of a cytochrome P450 of the
IIA subfamily from human liver microsomes.
Eur. J. Biochem. 200, 511-517 (1991)
CYP2A6 human
PIR A61272 (13 amino acids)
Yun, C.H., Shimada, T. and Guengerich, F.P.
Purification and characterization of human liver microsomal
cytochrome P-450 2A6.
Mol. Pharmacol. 40, 679-685 (1991)
CYP2A6v2 human
GenEMBL U22027(7215bp)
Fernandez-Salguero,P., Hoffman,S.M., Cholerton,S., Mohrenweiser,H.,
Raunio,H., Rautio,A., Pelkonen,O., Huang,J.D., Evans,W.E.,
Idle,J.R. and Gonzalez, F.J.
A genetic polymorphism in coumarin 7-hydroxylation: sequence of the
human CYP2A genes and identification of variant CYP2A6 alleles.
Am. J. Hum. Genet. 57, 651-660 (1995)
CYP2A7 human
GenEMBL U22029(2282bp)
Fernandez-Salguero,P., Hoffman,S.M., Cholerton,S., Mohrenweiser,H.,
Raunio,H., Rautio,A., Pelkonen,O., Huang,J.D., Evans,W.E.,
Idle,J.R. and Gonzalez, F.J.
A genetic polymorphism in coumarin 7-hydroxylation: sequence of the
human CYP2A genes and identification of variant CYP2A6 alleles.
Am. J. Hum. Genet. 57, 651-660 (1995)
CYP2A7 baboon (Papio sp.)
Swiss P80055 (20 amino acids) PIR S21737 (20 amino acids)
Purification of two cytochrome P450 isozymes related to CYP2A
and CYP3A gene families from monkey (baboon, Papio papio)
liver microsomes. Cross reactivity with human forms.
Dalet-Beluche I., Boulenc X., Fabre G., Maurel P., Bonfils C.
Eur. J. Biochem. 204, 641-648 (1992)
MLASGLLLVALLACLTVMVL
100% to CYP2A7 human
CYP2A7PTX human (retired name see CYP2A18PN)
GenEMBL U22030(1192bp)
Fernandez-Salguero,P., Hoffman,S.M., Cholerton,S., Mohrenweiser,H.,
Raunio,H., Rautio,A., Pelkonen,O., Huang,J.D., Evans,W.E.,
Idle,J.R. and Gonzalez, F.J.
A genetic polymorphism in coumarin 7-hydroxylation: sequence of the
human CYP2A genes and identification of variant CYP2A6 alleles.
Am. J. Hum. Genet. 57, 651-660 (1995)
There are two human pseudogenes of 2A7 on chromosome 19. They are
Located adjacent to each other. This one is telomeric.
CYP2A7PCX human (retired name see CYP2A18PN)
GenEMBL U22044(1192bp)
Fernandez-Salguero,P., Hoffman,S.M., Cholerton,S., Mohrenweiser,H.,
Raunio,H., Rautio,A., Pelkonen,O., Huang,J.D., Evans,W.E.,
Idle,J.R. and Gonzalez, F.J.
A genetic polymorphism in coumarin 7-hydroxylation: sequence of the
human CYP2A genes and identification of variant CYP2A6 alleles.
Am. J. Hum. Genet. 57, 651-660 (1995)
There are two human pseudogenes of 2A7 on chromosome 19. They are
Located adjacent to each other. This one is centromeric.
CYP2A8 Mesocricetus auratus (hamster)
GenEMBL M63788 M34446 M34447 (1771bp)
Lai,T.S. and Chiang, J.Y.L.
Cloning and characterization of two major 3-methylcholanthrene inducible hamster
liver cytochrome P-450s.
Arch. Biochem Biophys. 283, 429-439 (1990)
clone MC1 note: M34446 is incorrectly included in this GenBank entry
and in the 2A9 entry. M34446 should only be in the CYP1A2 hamster entry.
CYP2A9 Mesocricetus auratus (hamster)
GenEMBL M63789 M34446 M34448 (918bp)
Lai,T.S. and Chiang, J.Y.L.
Cloning and characterization of two major 3-methylcholanthrene inducible hamster
liver cytochrome P-450s.
Arch. Biochem Biophys. 283, 429-439 (1990)
clone MC1-81 3 prime end
note: M34446 is incorrectly included in this GenBank entry
and in the 2A8 entry. M34446 should only be in the CYP1A2 hamster entry.
CYP2A9 Syrian hamster
GenEMBL D86953
Kurose,K., Tohkin,M., Ushio,F. and Fukuhara,M.
Cloning and characterization of syrian hamster testosterone
7alpha-hydroxylase, CYP2A9
Arch. Biochem. Biophys. 351, 60-65 (1998)
clone name P450SH2A-1
1 amino acid difference with MC1-81 of Lai and Chiang (incomplete seq.)
CYP2A10 rabbit
GenEMBL L10236 (1641bp) Swiss Q05555 (494 amino acids)
Peng.H.-M., Coon,M.J. and Ding,X.
Isolation and heterologous expression of cloned cDNAs
for two rabbit nasal microsomal proteins CYP2A10 and CYP2A11
that are related to nasal microsomal cytochrome P-450 form a.
J. Biol. Chem. 268,17253-17260 (1993)
CYP2A10/11 rabbit
PIR A31944 (23 amino acids)
Ding, X. and Coon, M.J.
Purification and characterization of two unique forms of
cytochrome P-450 from rabbit nasal microsomes.
Biochemistry 27, 8330-8337 (1988)
CYP2A11 rabbit
GenEMBL L10237 (2484bp) Swiss Q05556 (494 amino acids)
Peng.H.-M., Coon,M.J. and Ding,X.
Isolation and heterologous expression of cloned cDNAs
for two rabbit nasal microsomal proteins CYP2A10 and CYP2A11
that are related to nasal microsomal cytochrome P-450 form a.
J. Biol. Chem. 268, 17253-17260 (1993)
Cyp2a12 mouse
GenEMBL L06463 (1665bp) PIR S32491 (492 amino acids)
Iwasaki,M., Juvonen,R., Lindberg,R. and Negishi,M.M.
Site-directed mutagenesis of mouse steroid 7 alpha-
hydroxylase cytochrome P-450 (7 alpha): Role of residue
209 in determining steroid-cytochrome P-450 interaction.
Biochemical J. 291, 569-573 (1993)
Note: called 7 alpha hydroxylase, but this sequence is very
different from CYP7 sequences. It is actually a 2A sequence.
Cyp2a12-de1b2b mouse
GenEMBL NW_000310 (52646-53186) also NT_039413.1 - strand
note: nuc. numbering same in both
detritus exons 1 and 2 = s in Figure 2B
Nelson et al. Pharmacogenetics 14, 1-18 (2004)
Between 2a12 and 2f2
Old name Cyp2a20p
53186 MTLS 53175
53173 MLLVAVLTCFIAMITMSVLR*KKLLGKMPPGPTPLPFLGNFLELDTKKFYDSFLRVVGREM 52988
52810 IREWYGPVFTVHLGTYSAVVPWGYDVVKETLVDQAEQFSGRGEQAFLDWFFKGYG 52646
CYP2A13 human
GenEMBL U22028(8778bp)
Fernandez-Salguero,P., Hoffman,S.M., Cholerton,S., Mohrenweiser,H.,
Raunio,H., Rautio,A., Pelkonen,O., Huang,J.D., Evans,W.E.,
Idle,J.R. and Gonzalez, F.J.
A genetic polymorphism in coumarin 7-hydroxylation: sequence of the
human CYP2A genes and identification of variant CYP2A6 alleles.
Am. J. Hum. Genet. 57, 651-660 (1995)
CYP2A13 Canis familiaris (dog)
XM_541608.2
91% to CYP2A13 human
There is a second CYP2A in dog that is 87% to CYP2A13
This seq is the probable ortholog of CYP2A13
Dog cluster order 2S(-), 2B(+), 2G(+), 2A25(+), 2A13(-), 2F(+), 2T(+)
Note: this seq is the same as Seq 2 sent by Tom Rushmore
On 6/28/05 except for 3 aa diffs
CYP2A13 Bos taurus (cow)
See cattle page for details
90% to 2A13 86% to 2A7
MLASGLLLVALLACLTIMVLMSVWRQRNLKGKLPPGPTPLPFIGNYLQLNTEQMCNSLMK
ISEHYGPVFTV
HLGTRQIVVLCGYDAVKEALVDQAEEFSGRGKQATFDWLFKGYGVAFSNGERAKQLRRFS
ITTLRDFGVGKRGIEERIQEEAGFLIEAFRGTRS
AFIDPTFFLSRTVSNVISSIVFGDRFDYEDKEFLSLLRMMLGSFQFTATSTGQ
1 LYEMFYSVMKYLPGPQQQAFKELQGLEDFIAKKVEQNQRTLDPNSPRDFIDSFLIRMQEEKENPNTEFYRK 177
178 NLVMTTLNLFFAGTETVSTTMRDGFLLLMKHPDVEAKIHEEIDRVIGKNRQPKFEDRAKM 357
358 PYTEAVIHEIQRFGDMIPMGLARRVTKDTKFRDFLLPKGTEVFPMLGSVLRDPKFFSNPR 537
538 DFNPQHFLDEKGQFKKSDAFVPFSIGKRYCFGESLARMELFLFFTTIMQNFRFKSPQS 711
712 PQDINVSPKLVGFATIPPNYTMSFLPR*
CYP2A13 frag. Bos taurus (cow)
PIR A35704 (18 amino acids)
Lazard, D., Tal, N., Rubinstein, M., Khen, M., Lancet, D. and
Zupko, K.
Identification and biochemical analysis of novel olfactory-specific
cytochrome P-450IIA and UDP-glucuronosyl transferase
Biochemistry 29, 7433-7440 (1990)
MXYLPGPQQQAFKELQGL
1 aa diff to human CYP2A13 and one uncalled amino acid
CYP2A13 horse
GenEMBL XM_001499763
Heather Knych
Submitted to nomenclature committee Oct. 21, 2007
88% to CYP2A13 human, 89% to dog CYP2A13
CYP2A14 Cricetulus griseus (Chinese hamster)
GenEMBL D86954
Fukuhara,M., Kurose, K., Aiba, N., Matsunaga, N., Omata, W., Kato, K.,
and Kimura, M.
A Major Phenobarbital-Inducible P450 Isozyme, CYP2A14, in the
Chinese Hamster Liver: Purification, Characterization, and cDNA
Cloning"
Arch. Biochem. Biophys. 359, 241-248 (1998)
clone P450CH2A-2 85% identical to 2A3 and 2a5
CYP2A15 Cricetulus griseus (Chinese hamster)
GenEMBL AB022916
Kouichi Kurose, Emi Isozaki, Masahiro Tohkin, and Morio Fukuhara
Cloning and expression analysis of a new member of the cytochrome
P450, CYP2A15 from the Chinese hamster, encoding testosterone 7alpha-
Hydroxylase.
Archives of Biochemistry and Biophysics (1999) Vol. 371 pp270-276
91% identical to CYP2A9
CYP2A16 Mesocricetus auratus (Syrian hamster)
GenEMBL D86952
Masahiro Tohkin, Kouichi Kurose, Emi Isozaki, and Morio Fukuhara
Molecular cloning, heterologous expression, and characterization of
a novel member of CYP2A in Syrian hamster"
Biochimica et Biophysica Acta (1999) Vol.1446 pp438-442
94% identical to CYP2A3
CYP2A17 Cricetulus griseus (Chinese hamster)
No accession number
Kouichi KUROSE
86% identical to CYP2A14
submitted to nomenclature committee 11/29/99
CYP2A18PC human pseudogene
AC008537
Hoffman S.M.G., Nelson, D.R. and Keeney, D.S.
Organization, strtucture and evolution of the CYP2 gene cluster
On human chromosome 19.
Pharmacogenetics 11, 687-698 2001
C-terminal part of P450 only. This is the opposite end of the
pseudogene CYP2A18PN. This gene appears to be split by a 2B6, 2B7P1
insertion.
CYP2A18PN human pseudogene
AC008537
Hoffman S.M.G., Nelson, D.R. and Keeney, D.S.
Organization, strtucture and evolution of the CYP2 gene cluster
On human chromosome 19.
Pharmacogenetics 11, 687-698 2001
N-terminal part of P450 only. This is the opposite end of the
pseudogene CYP2A18PC. This gene appears to be split by a 2B6, 2B7P1
insertion. This name replaces the old designations CYP2A7PT
and CYP2A7PC. There now seems to be only one copy of this pair
in the sequenced human genome.
CYP2A18PN human pseudogene (formerly CYP2A7PT)
GenEMBL U22030(1192bp)
Fernandez-Salguero,P., Hoffman,S.M., Cholerton,S., Mohrenweiser,H.,
Raunio,H., Rautio,A., Pelkonen,O., Huang,J.D., Evans,W.E.,
Idle,J.R. and Gonzalez, F.J.
A genetic polymorphism in coumarin 7-hydroxylation: sequence of the
human CYP2A genes and identification of variant CYP2A6 alleles.
Am. J. Hum. Genet. 57, 651-660 (1995)
There are two human pseudogenes of 2A7 on chromosome 19. They are
Located adjacent to each other. This one is telomeric.
Note added 4/10/2001 This gene appears to be split by a 2B6, 2B7P1
insertion. This name replaces the old designations CYP2A7PT
and CYP2A7PC. There now seems to be only one copy of this pair
in the sequenced human genome.
CYP2A18PN human pseudogene (formerly CYP2A7PC)
GenEMBL U22044(1192bp)
Fernandez-Salguero,P., Hoffman,S.M., Cholerton,S., Mohrenweiser,H.,
Raunio,H., Rautio,A., Pelkonen,O., Huang,J.D., Evans,W.E.,
Idle,J.R. and Gonzalez, F.J.
A genetic polymorphism in coumarin 7-hydroxylation: sequence of the
human CYP2A genes and identification of variant CYP2A6 alleles.
Am. J. Hum. Genet. 57, 651-660 (1995)
There are two human pseudogenes of 2A7 on chromosome 19. They are
Located adjacent to each other. This one is centromeric.
Note added 4/10/2001 This gene appears to be split by a 2B6, 2B7P1
insertion. This name replaces the old designations CYP2A7PT
and CYP2A7PC. There now seems to be only one copy of this pair
in the sequenced human genome.
CYP2A19 Sus scrofa (pig)
GenEMBL AB052255
Misaki Kojima
Submitted to nomenclature committee Oct. 27, 2000
89% to human CYP2A13
clone name c7
Cyp2a20pX mouse
GenEMBL NW_000310 (52646-53186)
53186 MTLS (frameshift) MLLVAVLTCFIAMITMSVLR*KKLLGK
MPPGPTPLPFLGNFLELDTKKFYDSFLRVVLGREM (0) 52988
52810 IREWYGPVFTVHLGTYSAVVPWGYDVVKETLVDQAEQFSGRGEQAFLDWFFKGYG 52646
renamed Cyp2a12-de1b2b
Cyp2a21-ps mouse
GenEMBL NW_000308.1, NW_033707.1, NT_039411.1
93% to Cyp2a5
runs off end NW_000308.1|Mm7_WIFeb01_154 also on
NW_033707.1|MmUn_WIFeb01_40262
t in Figure 2B Nelson et al. Pharmacogenetics 14, 1-18 (2004)
between 2a22 and 2a12
NT_039411.1 + strand seq = 20,879bp runs off end
15607 FFLGKRGIEEHIQEEVGLLIDSFRKTNG 15690
15948 GAFIDTTFYLSRTVSNVISSIIFRDRFDYEDKEFLSLL*MMLGSFQFTATSMGQ 16109
17609 LYEMFSSVMKHLSGPQQQAFKELQGLEDFITKKVEHNQRTLDPNSPRDFIDSFLIRMLE 17785
19308 EKKNPNTEFYMKNLVLTTQNLFFAGTETVSTTLRYGFLLLMKHPDIE 19448
19888 AKVHKEIDWVTGRNWQPKYEDRMKMPYAEAVIHEIQRFADMIPMGLARRVTKDTKFRDFLLPK 20076
20678 GTEVFPMLGSVLKDPKFFFNPKDFNPKHFLDDKGQFKKSDAFVPFSIG 20821
Cyp2a22 mouse
GenEMBL NW_000308.1|Mm7_WIFeb01_154
Also on NT_039411.1 - strand
93% to Cyp2a12
between 2a5 and 2a12
NW_000308.1
MLGSGLLLVAILVFLSVMVLVSVWQQKIRGKLPPGPIPLPFIGNYLQLNRKDVYSSITQ 392
LQEHYGPVFTIHLGPRRVVVLYGYDAVKEALEDNAEEFSGRGEQATFNTLFKGYG 834
VTFSNGERAKQLRRFSIATLKDFGLGKRGMEERIQEEAGCLIKMLQGTC 1495
GAPIDPTMYLSKTVSNVISSIVFGDRFNYEDKEFLSLLQMMSQMNQFAASPTGQ 1874
LYDMFHSVMKYLPGPQQQIIKDSHKLEDFMIQKVKHNHSTLDPNSPRGFIDSFLIHMQK 3263
EKNFNSEFHMKNLVMTSLNLFFAGSETVSSLLRYGFLLLMKHPDVE 4834
AKVHEEIDRVIGRNRQPQYEDHMKMPYTQAVIHEIQR 5365
FSNFAPLGIPRRITKDTSFRGFFLPK 5443
GTDVFPIMGSLMIDPKFFSSPKDFNPQHFLDDKGQLKKIPAFLPFSI 6101
GKRSCLGYSLGKMQLFLFFTTILQNFRFKFPRKLEDINESPKPEGFTRIIP 7191
KYTMSFVPI* 7221
Cyp2a22-de1b2b mouse
GenEMBL NW_011833.1|MmUn_WIFeb01_20427
between 2a22 and 2a5
93% to Cyp2a12-de1b2b
old name = Cyp2a23p
u in Figure 2B Nelson et al. Pharmacogenetics 14, 1-18 (2004)
MLLVAILTCFIAMITMSVLR*RKVLGKIPPGPTPLPFLGNFLELDTKKFYDSFLRV
VLGREM
IRELYGPVFTVHLGTHSAVVPWGYDVVKEALVDQAEQFSGRGEQAFLDWFFKDYG
CYP2A23 Macaca mulatta (rhesus monkey)
No accession number
Brian A. Carr, Merck & Co. Inc.
Submitted to nomenclature committee 4/22/2004
93% to CYP2A13, 92% to CYP2A6 human, possible ortholog of CYP2A13
CYP2A23 Macaca fasicularis (cynomolgus monkey)
No accession number
Yasuhiro Uno
Submitted to nomenclature committee 1/11/2005
Clone name mfCYP2A#1_27B2
98% to 2A23 Macaca mulatta 8 aa diffs
note 2A23 and 2A24 are very similar to 2A6 and 2A13, but I
cannot assign orthologs without mapping data.
CYP2A24 Macaca mulatta (rhesus monkey)
No accession number
Brian A. Carr, Merck & Co. Inc.
Submitted to nomenclature committee 4/22/2004
94% to CYP2A6, 93% to CYP2A13 human, possible ortholog of CYP2A6
CYP2A24 Macaca fasicularis (cynomolgus monkey)
No accession number
Yasuhiro Uno
Submitted to nomenclature committee 1/11/2005
Clone name mfCYP2A#2_2-G10
98% to 2A24 Macaca mulatta 8 aa diffs
note 2A23 and 2A24 are very similar to 2A6 and 2A13, but I
cannot assign orthologs without mapping data.
CYP2A23/24 Macaca fascicularis (cynomolgus monkey)
PIR S36874 (13 amino acids)
Ohmori, S., Horie, T., Guengerich, F.P., Kiuchi, M.and Kitada,M.
Purification and characterization of two forms of hepatic microsomal
cytochrome P450 from untreated cynomolgus monkeys.
Arch. Biochem. Biophys. 305, 405-413 (1993)
Identical to first 13 aa of CYP2A23 or CYP2A24
MLASGLLLVALLA
CYP2A25 Canis familiaris (dog)
XM_541607.2
87% to CYP2A13 human
There is a second CYP2A in dog that is 91% to CYP2A13
That seq is the probable ortholog of CYP2A13
Dog cluster order 2S(-), 2B(+), 2G(+), 2A25(+), 2A13(-), 2F(+), 2T(+)
Note: this seq is the same as Seq 1 sent by Tom Rushmore
On 6/28/05 except for a short frameshifted region
2B Subfamily
CYP2B1 or 2 rat
PIR A92255 (22 amino acids) B92255 (22 amino acids)
Botelho, L.H., Ryan, D.E. and Levin, W.
Amino acid compositions and partial amino acid sequences of
three highly purified forms of liver microsomal cytochrome
P-450 from rats treated with polychlorinated biphenyls,
phenobarbital, or 3-methylcholanthrene.
J. Biol. Chem. 254, 5635-5640 (1979)
CYP2B1 or 2 rat
PIR A60822 (20 amino acids)
Amelizad, Z., Narbonne, J.F., Wolf, C.R., Robertson, L.W. and
Oesch, F.
Effect of nutritional imbalances on cytochrome P-450 isozymes
in rat liver.
Biochem. Pharmacol. 37, 3245-3249 (1988)
CYP2B2 rat
GenEMBL S51970 (2946bp)
Hoffmann,M., Mager,W.H., Scholte,B.J., Civil,A. and Planta,R.J.
Analysis of the promoter of the cytochrome P-450 2B2 gene in the
rat.
Gene Expr. 2, 353-363 (1992)
promoter region, no coding sequence
CYP2B2 rat
GenEMBL L28169 (1401bp)
Shephard,E.E.A.
unpublished (1993)
promoter region
CYP2B2 rat
GenEMBL I00525 (427bp)
White,P.C., Dupont,B. and New,M.I.
Genetic probe used in the detection of adrenal hyperplasia
Patent: US 4720454-A 3 19-JAN-1988
Includes I-helix region
CYP2B3 rat
GenEMBL U16209 to U16214
Jean,A., Reiss,A., Desrochers,M., Dubois,S., Trottier,E., Trottier,Y.,
Wirtanen,L., Adesnik,M., Waxman,D.J. and Anderson,A.
Rat liver cytochrome P450 2B3: structure of the CYP2B3 gene and
immunological identification of a constitutive P450 2B3-like protein in
rat liver.
DNA Cell Biol. 13, 781-792 (1994)
CYP2B3-se1[9] rat
exon 9 100% match to 2B3 chr1 (+)frag a in fig below
81263180 GKRMCLGEGIARSELFLFFTTILQNYSVSSPVDPNTIDMTPKESGLAKVAPVYKICFVAR* 81263362
rat, mouse and human 2ABFGST clusters
CYP2B3-se2[1] rat
duplicate exon 1 100% match Chr1 (-)frag b in fig below
81308557 MDTSVLLLLAVLLSFLLFLVRGHAKVHGHLPPGPRPLPLLGNLLQMDRGGFRKSFIQ 81308387
rat, mouse and human 2ABFGST clusters
CYP2B4 rabbit
GenEMBL L10912 (2026bp)
Ryan,R., Grimm,S.W., Kedzie,K.M., Halpert,J.R. and
Philpot,R.M.
Expression and induction of cytochromes P450 2B and P450 4B,
identification of P450 2B-Bx, and functional comparison of four
highly related forms of P450 2B.
unpublished (1993)
CYP2B4 rabbit
GenEMBL S64259 (2028bp) PIR S35666 (491 amino acids)
Ryan,R., Grimm,S.W., Kedzie,K.M., Halpert,J.R. and Philpot,R.M.
Cloning, sequencing, and functional studies of
phenobarbital-inducible forms of cytochrome P450 2B and 4B
expressed in rabbit kidney
Arch. Biochem. Biophys. 304, 454-463 (1993)
CYP2B4 rabbit
Swiss P00177 PIR S31277 (491 amino acids) S31278 (491 amino acids)
PIR S31279 (491 amino acids)
Gasser R., Negishi M., Philpot R.M.
Primary structures of multiple forms of cytochrome P-450 isozyme 2
derived from rabbit pulmonary and hepatic cDNAs.
Mol. Pharmacol. 32, 22-30 (1988)
CYP2B5 rabbit
CYP2B6 human
PIR S04579 (139 amino acids) PIR S04580 (170 amino acids)
Miles, J.S.,Spurr, N.K., Gough, A.C., Jowett,T., McLaren, A.W.,
Brook,J.D. and Wolf, C.R.
A novel human cytochrome P450 gene (P450IIB): chromosomal
localization and evidence for alternative splicing.
Nuc. Acids Res. 16, 5783-5795 (1988)
CYP2B6 human
GenEMBL M29874
Yamano,S., Nhamburo,P.T., Aoyama,T., Meyer,U.A., Inaba,T.,
Kalow,W., Gelboin,H.V., McBride,O.W. and Gonzalez,F.J.
cDNA cloning and sequence and cDNA-directed expression of human
P450 IIB1: identification of a normal and two variant cDNAs derived
from the CYP2B locus on chromosome 19 and differential expression
of the IIB mRNAs in human liver.
Biochemistry 28, 7340-7348 (1989)
clone name hIIB1
CYP2B6 Macaca mulatta (rhesus monkey)
No accession number
Brian A. Carr, Merck & Co. Inc.
Submitted to nomenclature committee 4/22/2004
91% to CYP2B6, probable ortholog of CYP2B6
name changed to reflect orthology formerly CYP2B30
CYP2B6 Macaca fasicularis (cynomolgus monkey)
No accession number
Yasuhiro Uno
Submitted to nomenclature committee 1/11/2005
Clone name mfCYP2B6
3 aa diffs to CYP2B6 Macaca mulatta
CYP2B6 Macaca fasicularis (cynomolgus monkey)
No accession
Wu Zhicong
Submitted to nomenclature committee 10/30/2006
91% to human 2B6, 90% to human 2B7P1
4 amino acids diffs to Yasuhiro Unos seq
CYP2B6 Bos taurus (cow)
See cattle page for details
MELSMLLLFALLTGLLVLLARGRPKAHGRLPPGPRPLPFLGNLLQMDRKGLLKSFLR
FQQKYGDVFTVYLGPRPVVIICGTEAIREALVDQAEVFSGRAKIAVVDPIFQGY
GVIFANGERWKALRRFSLATMRDFGMGKRSVEERIQDEAQCLVEELRKSQ
GALQDPVFYFHSITANIICSIVFGKRFDYRDPEFLRLLELLFQSFVLISSLSSQ
LFELYSSFLKYFPGSHRQIYKNLQEINVFIGRSVEQHRETLDPNAPRDFIDCYLLRMEKDKSNPQSQFDHQN
LIMSVLSLFFAGTETTSTTLRYGFLLMLKYPHITERIQKEIDQVIGSYR
PALDDRAQMPYTDAVIHEIQRFADLIPIGVPHMVTKDTHFRGYILPK
GTEVYPVLSSALHESCYFEKPDDFNPDHFLDANGVVKKNDAFMPFSI
GKRICLGEGIARIELFLFFTTILQNFSVASPVAPEDIDLTPQESGVGNVPPNYRIQFLPRQRG*
CYP2B7P1 human
GenEMBL M29873
Yamano,S., Nhamburo,P.T., Aoyama,T., Meyer,U.A., Inaba,T.,
Kalow,W., Gelboin,H.V., McBride,O.W. and Gonzalez,F.J.
cDNA cloning and sequence and cDNA-directed expression of human
P450 IIB1: identification of a normal and two variant cDNAs derived
from the CYP2B locus on chromosome 19 and differential expression
of the IIB mRNAs in human liver.
Biochemistry 28, 7340-7348 (1989)
clone name hIIB3
This entry was originally made then discontinued as 2B7PX because an article by
Miles et al. Nuc. Acids res. 18, 189 (1990) showed evidence of alternative splicing
of CYP2B6. I thought that this explained the difference. However, on going back
and looking at the sequences and the EST data and mRNAs, there are clearly two
different genes in the 2B human subfamily. M29873 has an in frame stop codon,
making it a pseudogene.
CYP2B7P Bos taurus (cow)
See cattle page for details
stop codon same as in human 2B7
PALDDRAQMPYTDTVIHEIQRFADLISIGVSHMDAKDAHF*GYILPK
Cyp2b8 rat
Cyp2b9 mouse
GenEMBL M60267 to M60273, also AH000038
Lakso,M., Masaki,R., Noshiro,M. and Negishi,M.
Structures and characterization of sex-specific mouse cytochrome
P-450 genes as members within a large family. Duplication boundary
and evolution
Eur. J. Biochem. 195, 477-486 (1991)
Cyp2b9-de9b mouse
GenEMBL XM_145463, XP_145463, NT_039410.1
x in Figure 2B Nelson et al. Pharmacogenetics 14, 1-18 (2004)
detritus exon 9 between Cyp2a4 and Cyp2b9
old name = Cyp2b25p
NT_039410.1 - strand
196560 SGTRICLGEGIARSELFLFFTTILQ 196486
196484 NFSVSSPVAPKDIDITLKESGLAKIPPVYKISFLAH* 196374
Cyp2b10 mouse
GenEMBL M21856, PIR A60559 (15 amino acids)
Bornheim, L.M. and Correia, M.A.
Purification and characterization of a mouse liver cytochrome
P-450 induced by cannabidiol.
Mol. Pharmacol. 36, 377-383 (1989)
Note: the genome of mouse has only one sequence for Cyp2b10 and Cyp2b20. They are derived from the same gene. The Cyp2b10 mRNA M21856 appears to contain errors in the sequence. No exact match for it can be found in the mouse genome.
This mRNA has an extra exon called exon 8b (27 nucleotides in the heme binding peptide region). This appears to be an alternative splice variant of this gene.
The Cyp2b20 sequence matches the genomic sequence and represents the correct 2b10 sequence. The Cyp2b20 name has been discontinued and Cyp2b10 has been retained
since it is the older of the two names.
GenEMBL M21856 (sequence Cyp2b10 was based on)
MEPSVLLLLALLVGFLLLLARGHPKSRGNFPPGPRPLPLLGNLLQMDRGGLLKSLIQ
LREKYGDVFTVHLGPRPVVMLCGTDTIREALVGQAEAFSGRGTVAVVEPTFKEY
GVIFANGERWKTLRRFSLATMRDFGMGKRSVEERIQEEAQCLVEELRKS
QGAPLDPTFLFQCITANVICSIVFGERFEYTDRQFLRLLELFYQTFSLISSFSSQ
MFELFSGFLKYFPGAHRQISKNLQELLDYIGHSVERHKATLDPSVPRDFIDIYLLRMEK
EKSNQNAEFHHQNLMMSVLSLFFVGTETSSTTLHYGFLLMLKYPHVTEKVQKEIDQVIGS
HRLPTLDDRTKMPYSDAVIHEIQRFSDLIPIGVPHRVTKDTLFRGYLLPKNTEVYPIL
SSALHDPQYFEQPDSFNPDQFLDANGALKKSEAFLPFST
Exon 8b GQIFDQKSV
GKRICLGESIARSELFLFFTSILQNFSVASHVAPKDIDLTPKESGIGKIPPTYQICFLAR
GenEMBL AK028103 from RIKEN (corrected Cyp2b10/Cyp2b20 sequence)
MEPSVLLLLALLVGFLLLLARGHPKSRGNFPPGPRPLPLLGNLL
QMDRGGLLKSFIQLREKYGDVFTVHLGPRPVVMLCGTDTIREALVGQAEAFSGRGTVA
VVEPTFKEYGVIFANGERWKTLRRFSLATMRDFGMGKRSVEERIQEEAQCLVEELRKS
QGAPLDPTFLFQCITANIICSIVFGERFEYTDRQFLRLLELFYQTFSLISSFSSQMFE
LFSGFLKYFPGAHRQISKNLQELLDYIGHSVEKHRATLDPSVPRDFIDIYLLRMEKEK
SNQHTEFHHQNLMMSVLSLFFAGTETSSTTLRYGFLLMLKYPHVAEKVQKEIDQVIGS
HRLPTLDDRTKMPYTDAVIHEIQRFSDLIPIGVPHRVTKDTMFRGYLLPKNTEVYPIL
SSALHDPQYFEQPDSFNPDHFLDANGALKKSEAFLPFSTGKRICLGESIARNELFLFF
TSILQNFSVASHVAPKDIDLTPKESGIGKIPPTYQICFLAR
CYP2B11 dog
CYP2B12 rat
GenEMBL S48369 X63545 (2528bp) Swiss P33272 (492 amino acids)
PIR S27160 (492 amino acids)
Friedberg,T., Grassow,M.A., Bartlomowicz-Oesch,B., Siegert,P,
Arand,M., Adesnik,M. and Oesch,F.
Sequence of a novel cytochrome CYP2B cDNA coding for a
protein which is expressed in a sebaceous gland, but not in the liver.
Biochem. J. 287, 775-783 (1992)
CYP2B12-de9b rat
exon 9 Chr1 (-) frag c in fig. below
81829155 GKFICLGEGIG*NESFIFFTGILQNLSLASPVAPENIDLTPIKSGAGKIPSTYQIHILSR 81829012
rat, mouse and human 2ABFGST clusters
Cyp2b13 mouse
GenEMBL M60352 to M60358, also AH000037, NT_039410.1
Lakso,M., Masaki,R., Noshiro,M. and Negishi,M.
Structures and characterization of sex-specific mouse cytochrome
P-450 genes as members within a large family. Duplication boundary
and evolution.
Eur. J. Biochem. 195, 477-486 (1991)
Cyp2b13-de1b2b7b mouse
GenEMBL NT_039410.1 + strand
y in Figure 2B Nelson et al. Pharmacogenetics 14, 1-18 (2004)
detritus exons 1,2,7 between Cyp2b13 and Cyp2b26-ps
43894 XXXXXXDIFYMGAQPLLVLCGYEV*WEAPVDHSEVFLVYEDKAIIDPSSKKW 44031 ex 1
44377 XXFFVNGKPWNIVN*FLLTTTKDFEWKKRSIDNQIKVETLDLLLEC*KPHGDP 44529 ex 2
48130 LPVFVHWAQKPYTQASIHEIWRYGDFTHIG 48219 ex 7
CYP2B14X rat
discontinued number see CYP2B16P
CYP2B14P rat
GenEMBL U33540
Eric Trottier, Stéphane Dubois, Andréa Jean and Alan
Anderson
Identification of CYP2B14P and CYP2B16P, two apparent pseudogenes in
the rat cytochrome P450 2B (CYP2B) subfamily.
Biochemical Pharmacology, 52, 963-965 (1996)
exon 1, add Chr1 (+) exons 7,8,9 72% to 2B21 to this pesudogene
81706300 MKPNVLLLLAILLSFLLFLVRGHAKVHGHLPPGPRPLPILGNLLQMDRGGLLQSF 81706464
81728276 EKVQKEIGEVTGSHWFPILYSSKIPNTEAVIPEIQR 81728383
81728385 FSDLSSVVLPQRVTKDTFFQGFLLHK 81728462
81728634 NTEVYPILSSVLHDPQ 81728681
81728681 VLEYPVTFNPEHFLDANGALKKNEAFTPFSR 81728773
CYP2B15 rat
GenEMBL D17343 to D17349
Nakayama,K., Suwa,Y., Mizukami,Y., Sogawa,K. and Fujii-
Kuriyama, Y.
Cloning and sequencing of a novel rat cytochrome P450 2B-encoding
gene.
Gene 136, 333-336 (1993)
most similar to 2B12, 89% identical
MELGVLLLLTFTVGFLLLLASQNRPKTHGHLPPGPRPLPFLGNLLQMNRRGLLRSFMQLQ
EKYGDVFTVHLGPRPVVILCGTDTIREALVDQAEAFSGRGTVAVLHPVVQGYGVIFANGE
RWKILRRFSLVTMRNFGMGKRSVEERIKEEAQCLVEELKKYKALLNPTSIFQSIAANIIC
SIVFGERFDYKDHQFLRLLDLIYQTFSLMGSLSSQVFELFSGFLKYFPGVHKQISKNLQE
ILNYIDHSVEKHRATLDPNTPRDFINTYLLRMEKEKSNHHTEFHHQNLVISVLSLFFTGT
ETTSTTLRYSFLIMLKYPHVAEKVQKEIDQVIGSHRLPTLDDRTKMPYTDAVIHEIQRFA
DLIPIGLPHRVTNDTMFLGYLLPKNTEVYPILSSALHDPRYFDHPDTFNPEHFLDVNGTL
KKSEAFLPFSTGKRICLGEGIAQNELFIFFTAILQNFSLASPVAPEDIDLSPINSGISKI
PSPYQIHFLSRCVG
CYP2B16P rat
GenEMBL U33541 to U33546
Eric Trottier, Stéphane Dubois, Andréa Jean and Alan Anderson
Identification of CYP2B14P and CYP2B16P, two apparent pseudogenes in the rat
cytochrome P450 2B (CYP2B) subfamily.
Biochemical Pharmacology, 52, 963-965 (1996)
note: previously called CYP2B14 in 1993 update. This gene has a complete
coding sequence but there is a defect in the splice junction in intron 1.
Exon 1 MEPSVLLLLAVLLSFLLLLVRGHAKIHGRLPPGPCPVPLLGNLLQMDRRGLLKSFIQLR
Exon 2 EKYGDVFTVHLGLRPVVVLCGTQTIREALVDHAEAFSGRGTIAGLEPVFQDYG
Exon 3 IFFSSGEQWKTLRRFSMATMRDFGMRKKSVEERIKEESQCLVEELKKYQG
Exon 4 APLDPTFLFQCITSNIICSIVFGECFDYTDHQFLHLLDLMYQTFSLLSSIFSQ
Exon 5 VFELFPGVLKYFPGAHRQISRNLHEILDFIGQSVEKHRATLDPNAPRDFIYTYLLHMEK
Exon 6 QKSNHYTEFHHWNLLSSVLSLFFAGTETSSTTLRYGFLIMLKYPHI
Exon 7 EKVQKEIDCVIGSHRLPTLDDRSKMPYTEAVIHEIQRFSDLAPIGTPHRVIKDTIFRGYLLPK
Exon 8 QNTEVFPILSSVLHDPQYFEQPDIFNLQHFLDANGALKIIEAFLPFSTGK
Exon 9 TGKRICLGESIARNELFLFFTTILQNFSVSSPVAPKDIDLTPKESGIGRIPQVYQICFLA
CYP2B17/2B6 Cercopithecus aethiops (African green monkey)
PIR JT0676 (491 amino acids)
Ohmori, S.; Sakamoto, Y.; Nakasa, H.; Horie, T.; Saito, K.; Kitada, M.
Nucleotide and amino acid sequences of monkey P450 2B gene
subfamily.
Unpublished
91% to human 2B6 probable ortholog
CYP2B18 guinea pig
no accession number (437 amino acids)
Oguri, K.
submitted to nomenclature committee
Cyp2b19 mouse
GenEMBL AF047529, also NT_039410.1 + strand
Diane Keeney, D.S. (1998) The Novel Skin-Specific Cytochrome P450
Cyp2b19 Maps to Proximal Chromosome 7 in the Mouse, near a Cluster of
Cyp2 Family Genes.
Genomics 53, 417-419.
Between 2b23 and 2g1
Cyp2b19-de7b8b9b mouse
GenEMBL NT_039410.1
old name = Cyp2b24p
v in Figure 2B Nelson et al. Pharmacogenetics 14, 1-18 (2004)
detritus exons 7,8,9 between 2b19 and 2b23
NT_039410.1 + strand
695673 EKVQKETDQVIGSHQLPTLDDRTKMPYTDTVIHEIQRFSDLAAIDLPHRVTIHTLSQVYLLPK 695861
696036 NTEVYPILSSVLLDP 696080
696083 QYFEQLDCFNPEHFLDANGTLKKSEAFLPFST 696178
702801 GKHVCLGKGIAHNELFLFFPTILQNFPVSVPLAPKDIDITPKESGTGKIPQCTRSAS 702971
Cyp2b20X mouse
GenEMBL X99715(1416bp)
Damon,M., Fautrel,A., Marc,N., Guillouzo,A. and Corcos,L.
Isolation of a new mouse cDNA clone: hybrid form of cytochrome P450
2b10 and NADPH-cytochrome P450 oxidoreductase
Biochem. Biophys. Res. Commun. 226 (3), 900-905 (1996)
This clone has a part of the NADPH cytochrome P450 reductase on the
opposite strand at the end of the P450 sequence.
note: this sequence was accidentally given the name Cyp2b19. That
name is assigned to a mouse keratinocyte P450 cloned by Diane Keeney.
The reductase sequence at the end of this gene seems to be a cloning
error, because it cannot be found in the genomic DNA sequence.
Cyp2b20 has been merged with Cyp2b10. Though the Cyp2b20 sequence
is more like the genomic sequence, the Cyp2b10 name has precedence.
GenEMBL AF128849
Marc,N., Damon,M., Fautrel,A., Guillouzo,A. and Corcos,L.
Isolation of a cyp2b10-like cDNA and of a clone derived from a
cyp2b10-like pseudogene
Biochem. Biophys. Res. Commun. 258 (1), 11-16 (1999)
This sequence is 100% identical to Cyp2b20 and 97% identical to
Cyp2b10
MEPSVLLLLALLVGFLLLLARGHPKSRGNFPPGPRPLPLLGNLL
QMDRGGLLKSFIQLREKYGDVFTVHLGPRPVVMLCGTDTIREALVGQAEAFSGRGTVA
VVEPTFKEYGVIFANGERWKTLRRFSLATMRDFGMGKRSVEERIQEEAQCLVEELRKS
QGAPLDPTFLFQCITANIICSIVFGERFEYTDRQFLRLLELFYQTFSLISSFSSQMFE
LFSGFLKYFPGAHRQISKNLQELLDYIGHSVEKHRATLDPSVPRDFIDIYLLRMEKEK
SNQHTEFHHQNLMMSVLSLFFAGTETSSTTLRYGFLLMLKYPHVAEKVQKEIDQVIGS
HRLPTLDDRTKMPYTDAVIHEIQRFSDLIPIGVPHRVTKDTMFRGYLLPKNTEVYPIL
SSALHDPQYFEQPDSFNPDHFLDANGALKKSEAFLPFSTGKRICLGESIARNELFLFF
TSILQNFSVASHVAPKDIDLTPKESGIGKIPPTYQICFLAR
Cyp2b20X mouse
GenEMBL AK028103 100% identical to AF128849
Now renamed Cyp2b10 (the corrected sequence)
MEPSVLLLLALLVGFLLLLARGHPKSRGNFPPGPRPLPLLGNLL
QMDRGGLLKSFIQLREKYGDVFTVHLGPRPVVMLCGTDTIREALVGQAEAFSGRGTVA
VVEPTFKEYGVIFANGERWKTLRRFSLATMRDFGMGKRSVEERIQEEAQCLVEELRKS
QGAPLDPTFLFQCITANIICSIVFGERFEYTDRQFLRLLELFYQTFSLISSFSSQMFE
LFSGFLKYFPGAHRQISKNLQELLDYIGHSVEKHRATLDPSVPRDFIDIYLLRMEKEK
SNQHTEFHHQNLMMSVLSLFFAGTETSSTTLRYGFLLMLKYPHVAEKVQKEIDQVIGS
HRLPTLDDRTKMPYTDAVIHEIQRFSDLIPIGVPHRVTKDTMFRGYLLPKNTEVYPIL
SSALHDPQYFEQPDSFNPDHFLDANGALKKSEAFLPFSTGKRICLGESIARNELFLFF
TSILQNFSVASHVAPKDIDLTPKESGIGKIPPTYQICFLAR
Cyp2b20p1X mouse
GenEMBL AF129405
Marc,N., Damon,M., Fautrel,A., Guillouzo,A. and Corcos,L.
Isolation of a cyp2b10-like cDNA and of a clone derived from a
cyp2b10-like pseudogene
Biochem. Biophys. Res. Commun. 258 (1), 11-16 (1999)
This sequence is 100% identical to Cyp2b20 from amino acid 64 on
This seq is partial, starting at amino acid 60 with a stop codon
at amino acid 63. Full length cDNAs AK028103 and AF128849 do not
have this stop codon and it is not found in genomic DNA.
This probably represents a sequence derived from the Cyp2b10 gene.
CYP2B21 rat
GenEMBL AF159245
Nicola Brookman Amissah and Peter Swann
CYP2B22 Sus scrofa (pig)
GenEMBL AB052256
Misaki Kojima
Submitted to nomenclature committee Oct. 27, 2000
78% to rabbit CYP2B4
clone name c780
Cyp2b23 mouse
NW_000307 618973-640139, also XM_145466
Haoyi Wang, Kyle Donley, Diane Keeney, Susan Hoffman
Next to Cyp2b19-de7b8b9b and 2b19 on chr 7
Cyp2b24pX mouse
NW_000307 692575-699876
Haoyi Wang, Kyle Donley, Diane Keeney, Susan Hoffm