Cryptococcus neoformans P450s

March, 2, 2005

This is a collection of five P450s taken from the Genbank chromosome entries.

They were found by text search for P450, not by blast searching.

There may be more of these P450s in this species. I still have to look for them.

See the genome manuscript at Science 307, 1321-1324 (2005) [25 Feb. issue]

>AAEY01000001 945048 bp DNA linear PLN 13-JUL-2004

DEFINITION Cryptococcus neoformans var. neoformans B-3501A chromosome 1

CDS join(94307..94462,94559..94798,94853..94930,94980..95351,

95399..95571,95626..95752,95807..95820,95867..96284,

96343..96417)

/locus_tag="CNBA0300"

/note="Match to ESTs gb|CF192489.1|CF192489,

gb|CF192488.1|CF192488, gb|CF191439.1|CF191439; Similar to

gi|7021480|gb|AAF35366.1| lanosterol 14 alpha-demethylase

[Filobasidiella neoformans], FASTA scores: opt: 3704, E():

0, (100.000% identity (100.000% similar) in 550 aa overlap

(1-550:1-550)); HMMPfam hit to p450, Cytochrome P450,

score: 185.7, E(): 9.4e-53"

/codon_start=1

/product="hypothetical protein"

/protein_id="EAL23379.1"

/db_xref="GI:50260729"

/translation="MSAIIPQVQQLLGQVAQFFPPWFAALPTSLKVAIAVVGIPALII

GLNVFQQLCLPRRKDLPPVVFHYIPWFGSAAYYGEDPYKFLFECRDKYGDLFTFILMG

RRITVALGPKGNNLSLGGKISQVSAEEAYTHLTTPVFGKGVVYDCPNEMLMQQKKFIK

SGLTTESLQSYPPMITSECEDFFTKEVGISPQKPSATLDLLKAMSELIILTASRTLQG

KEVRESLNGQFAKYYEDLDGGFTPLNFMFPNLPLPSYKRRDEAQKAMSDFYLKIMENR

RKGESDHEHDMIENLQSCKYRNGVPLSDRDIAHIMIALLMAGQHTSSATSSWTLLHLA

DRPDVVEALYQEQKQKLGNPDGTFRDYRYEDLKELPIMDSIIRETLRMHAPIHSIYRK

VLSDIPVPPSLSAPSENGQYIIPKGHYIMAAPGVSQMDPRIWQDAKVWNPARWHDEKG

FAAAAMVQYTKAEQVDYGFGSVSKGTESPYQPFGAGRHRCVGEQFAYTQLSTIFTYVV

RNFTLKLAVPKFPETNYRTMIVQPNNPLVTFTLRNAEVKQEV

CYP51F1 56% to CYP51F1 white rot

>AAEY01000006 265665 bp DNA linear PLN 13-JUL-2004

DEFINITION Cryptococcus neoformans var. neoformans B-3501A chromosome 2

CDS join(176011..176192,176251..176557,176610..176930,

176967..177117,177172..177277,177339..177663)

/locus_tag="CNBB0620"

/note="HMMPfam hit to p450, Cytochrome P450, score: -6.0,

E(): 4.5e-11"

/codon_start=1

/product="hypothetical protein"

/protein_id="EAL22841.1"

/db_xref="GI:50260180"

/translation="MLKQLSFQSPRTIEIIPLALVVLVVWLFRVFVYKPFTSPLKNVP

CPPGGTSSQGHIAEIMNLQGTEVNQWIKTYGSTFMVRGPFGVHHRIFTVDPRALSHVL

QHTNIYTKSDLLRGLVRRYMKEGLIVAEGERHKAQRKVSQKLFSMGGLKSMGQVVQDK

SNQLRDILLNLCTNPTASNPYSPVNRTLPPGSREVDVYSTASRCTFDLIGSIGVDHQF

DSIGDWEGSGGKLFHKYERMQLLCPGTTGFRMLLSLAWPLIDKIWVNNIFPLPSENTK

RVNDAMGSLEKFAKEKMIERQQELLTIDSKKGDVPDRGDLLTLMLRHNMTRKISPADK

LRDHEISGQLSTFMFAGSETTAGTISFGLCDLARHPDIQSRLRAEILECGDNLPFNQI

DELPYLDAVVKEIMRINPSLPGTVRQAQKDDIIPLAEPVILTNGKVVTDIHIRKGQL (0)

Note this seq is short on the end, most like white rot P450s

Add this sequence (yellow too long may have to delete)

VHVPIEHLHTSEHIWGPTAKEFNPSRFLSTTQPSAFSGRPTLASNPAATSSARRD

AVPNYVPEGPGIWPNFMTFIDGPRRCIGYKLAVMEIKTVIFTLLREFEIELMEGQHIFRWNM ()

MSNRPFVANTLRSKGSRLPLHFKLYKGEQQHEKTGGS*

CF712065.1 = mRNA

>AAEY01000013 1122915 bp DNA linear PLN 13-JUL-2004

DEFINITION Cryptococcus neoformans var. neoformans B-3501A chromosome 3

CDS complement(join(682935..682976,683028..683267,

683315..683534,683587..683638,683699..683837,

683893..683948,684005..684170,684228..684530,

684578..684721,684785..685150))

/locus_tag="CNBC2600"

/note="HMMPfam hit to p450, Cytochrome P450, score: 56.3,

E(): 5.8e-14"

/codon_start=1

/product="hypothetical protein"

/protein_id="EAL22122.1"

/db_xref="GI:50259449"

/translation="MKGGRFLLPALFKTLLLPPLIAALFIHHFPSLFPTFTALIYLIS

FPALYVFRSYASLVASSWKASALGAIDIPRVNGTWPLNIDILLNWAKSGTEEEVGRMM

VLMGRQYGGTYNTRVLGEDQIISSDPKVIKHVLIDDFDNFVKGQKFKERAQDFLGDGI

FNSDGDNWKFHRSFMRPFFQPKYISPLHFSTNVQNFFAKLPSYGKVFDMQAHIGQLAL

ELAIMWLCGEDMSRDTNNVAWTEEWKKAKGEISWAMTEAQKIVGKRVKIGTIWPLFEV

RHDPLERPMKVIRAFFRPIISRALNRKRQRCKLDNTEDVYMIDRLVEATDDIKLVEDQ

LINVLLASRDTLASLLTFSVYAIVLHPDIAVRLKDEVFTIANQECEVTKDTIRQLRYC

RAFINEVLRLFPPVPLNIRRTLRPSLLPTPNHLAYMPANTSIILATILMQRDPAVWGE

DAQVFNPDRWLEGGGLGKEKEGFMSWNLGPRMCLGQSFALAITHTFLVYFFRHIAHVG

TLVGHNTTVQLALDAQPPETVLPKEWMTPEGGDGRARGGRDKVWIVADVVLAIKGGLW

IRFGAEETE

Most like white rot P450s

>AAEY01000030 650090 bp DNA linear PLN 13-JUL-2004

DEFINITION Cryptococcus neoformans var. neoformans B-3501A chromosome 6

CDS complement(join(343289..343346,343418..343761,

343818..344502,344552..344740,344796..345005,

345059..345162))

/locus_tag="CNBF1100"

/note="Match to ESTs gb|CF191501.1|CF191501,

gb|CF188802.1|CF188802, gb|CF194425.1|CF194425; HMMPfam

hit to p450, Cytochrome P450, score: 43.1, E(): 2.4e-13"

/codon_start=1

/product="hypothetical protein"

/protein_id="EAL20298.1"

/db_xref="GI:50257593"

/translation="MESHTVLRPTAIPDLAAIKTWGLEGLTKAKFSFDTKTTTATILT

LILSLLVLEQLVYRAKKAHLPGAKWTIPVIGKFADSLNPTLANYKAQWNSGPLSAVSV

FNIFIVIGSSNEMARKILNSPNHAEPCLVASAKKVLLPENWVFLHGKVHADYRKALNV

LFTKQALSIYLPIQEKIYRSYFNKWMSDPAPAQQYMMKMRDLNMDTSLSVFIGPYLTE

AQKQEINDKYWLITISLELVNFPLAIPGTKVYNAIQARKIVMKYLSAASAASKIRMED

DDAEPECLLDHWVRAMILARRAKDDGEQTRLLSREYSDHEIAMVLLSFLFASQDAMSS

ALVYTFQLTADHPEVLEKVREEQYRVRGNDLERPLTLDMLDDMVYTRATIKEVLRFRP

PVIMVPYMTTKPFPVSPEYTAPKNSMIIPAFWNSLHDETCYPEPDRFLPERWLPQADG

SAPIADSKPQNYLVWGSGPHKCIGGQYASMHLAATLGTASVLMDWKHERTELSDEVQV

IAAIFPKDHCLLKFTPRAPPS

CYP61 sequence most like white rot CYP61

>AAEY01000032 550752 bp DNA linear PLN 13-JUL-2004

DEFINITION Cryptococcus neoformans var. neoformans B-3501A chromosome 6

CDS join(207402..207588,207646..208292,208345..208404,

208451..208547,208598..208805,208860..209050,

209105..209381,209438..209519)

/locus_tag="CNBF3400"

/note="HMMPfam hit to p450, Cytochrome P450, score: 137.2,

E(): 3.7e-38"

/codon_start=1

/product="hypothetical protein"

/protein_id="EAL20013.1"

/db_xref="GI:50257304"

/translation="MTMELLKVLHHGASQLFPNCIRSSPVACIVLYSFGGIAILLFTV

YLWLWPFQYAKLYFRNLPGPPSDSWFWGVVPTLIKSPPSVPHSMWTDEYGPTVRYRVA

LGAQRFLTIDPTALNYILSHADLFPKPSRVRKALSDLLGNELLTAEGHTHKKQRKALN

PSFSPAAVRGMIPVFYDKAYELKAKLLGIIEGDETEQASPTPCKEEDEVEGGKKIDVM

KYLGKTTLDVIGIVGFSYDFKALSEPRNELSEAYSKMFQAGMDANFWDFLRGAIPLVN

KLPNKRATEIAARKAVTLRISKKIVEDKKREVMSAHSEGLEKREDIGDDLLSILIKAN

MASDVKPEQKLSDEEVLDQITTFMLAGNETSSTALTWILYSLTQHPECQKRLREEVLA

VPDDRPSLETLNNLPYMDAVIREALRLHAPAPGTMREAKEDTVIPLSMPVIGRDGKQI

DSVKINKGTMVFIPIITVNTSPAIWGPDARVFNPDRHFKTSSDSFGGANMHVPGVWGN

MLSFLGGARNCIGYKLALAEISTILFVLIRSFEFQELKSKPEVEKKASVVMRPRIKGE

ESAGLQMPLMVKPLLM

Most like white rot fungal P450 on scaffold 7