>CYP34A8
B0213.14 499 aa
Length = 499
Score = 2442 (859.6 bits), Expect =
4.5e-257, P = 4.5e-257
Identities = 484/499 (96%), Positives =
484/499 (96%)
Query: 1
MLLILILLAVAAVLTANLWRARQKLPKGPTPLPLIGNFHQLFYLSWKTGSLVAAFNELKK 60
MLLILILLAVAAVLTANLWRARQKLPKGPTPLPLIGNFHQLFYLSWKTGSLVAAFNELKK
Sbjct: 1
MLLILILLAVAAVLTANLWRARQKLPKGPTPLPLIGNFHQLFYLSWKTGSLVAAFNELKK 60
Query: 61
QYGKVFTVWMGPKPSVYICDYDIAHETHVKRANIFGTRYSVGGMEYIREGRGIIGSNGDF 120
QYGKVFTVWMGPKPSVYICDYDIAHETHVKRANIFGTRYSVGGMEYIREGRGIIGSNGDF
Sbjct: 61
QYGKVFTVWMGPKPSVYICDYDIAHETHVKRANIFGTRYSVGGMEYIREGRGIIGSNGDF 120
Query: 121
WLEHRRFALMTLRNFGVGRTIMEDKIMDEYRYRFKDFKRTHFKNGAIQVNASSIFDLLVG 180
WLEHRRFALMTLRNFGVGRTIMEDKIMDEYRYRFKDFKRTHFKNGAIQVNASSIFDLLVG
Sbjct: 121
WLEHRRFALMTLRNFGVGRTIMEDKIMDEYRYRFKDFKRTHFKNGAIQVNASSIFDLLVG 180
Query: 181
SIINQLLVSERFEQDDQEFEKLKTSLAEALENISIIEGFLPLWVLKSPLMKWRTKITFAP 240
SIINQLLVSERFEQDDQEFEKLKTSLAEALENISIIEGFLPLWVLKSPLMKWRTKITFAP
Sbjct: 181
SIINQLLVSERFEQDDQEFEKLKTSLAEALENISIIEGFLPLWVLKSPLMKWRTKITFAP 240
Query: 241
---------------VAAIENGTHTLSEEGDDFVDAFIVKMEKDKKEGIDSSFTYVLSII 285
VAAIENGTHTLSEEGDDFVDAFIVKMEKDKKEGIDSSFT
Sbjct: 241 FDFIFELGNRGIQRRVAAIENGTHTLSEEGDDFVDAFIVKMEKDKKEGIDSSFT------
294
Query: 286
RKFSIACFSLETLAIDLFDLWQAGQETTSTTLTWACVCLLNHPEVVEKLRKELTEVTGGT 345
LETLAIDLFDLWQAGQETTSTTLTWACVCLLNHPEVVEKLRKELTEVTGGT
Sbjct: 295
---------LETLAIDLFDLWQAGQETTSTTLTWACVCLLNHPEVVEKLRKELTEVTGGT 345
Query: 346
RGVSLTDRTKTPYLNANINEFQRIASILNVNLFRVLEEDTTIDSQPVPAGALVTTNLSML 405
RGVSLTDRTKTPYLNANINEFQRIASILNVNLFRVLEEDTTIDSQPVPAGALVTTNLSML
Sbjct: 346
RGVSLTDRTKTPYLNANINEFQRIASILNVNLFRVLEEDTTIDSQPVPAGALVTTNLSML 405
Query: 406
HTDEEIFKNPQEFRPERFMENNNLEKRLIPFGIGKRACPGESLARAELFLITGNMILDYD 465
HTDEEIFKNPQEFRPERFMENNNLEKRLIPFGIGKRACPGESLARAELFLITGNMILDYD
Sbjct: 406
HTDEEIFKNPQEFRPERFMENNNLEKRLIPFGIGKRACPGESLARAELFLITGNMILDYD 465
Query: 466 LEPVGTLPKIETTTPFAPMKRPPVYDIRFVPRSQ
499
LEPVGTLPKIETTTPFAPMKRPPVYDIRFVPRSQ
Sbjct: 466
LEPVGTLPKIETTTPFAPMKRPPVYDIRFVPRSQ 499
Compare to seq below
>CYP34A7
B0213.12 499 aa
Length = 499
Score = 2193 (772.0 bits), Expect =
1.1e-230, P = 1.1e-230
Identities = 405/498 (81%), Positives =
456/498 (91%)
Query: 1
MLLILILLAVAAVLTANLWRARQKLPKGPTPLPLIGNFHQLFYLSWKTGSLVAAFNELKK 60
ML+ILIL+A+AAVLT NLWRARQKLP GPTPLP+IGNFHQLFY WK G LVA F++ +K
Sbjct: 1
MLIILILVAIAAVLTVNLWRARQKLPNGPTPLPIIGNFHQLFYNGWKYGGLVAGFDQFRK 60
Query: 61
QYGKVFTVWMGPKPSVYICDYDIAHETHVKRANIFGTRYSVGGMEYIREGRGIIGSNGDF 120
QYGKVFTVWMGP P+V ICD+D+AHETHVK+A+ FG RY+ G MEYIREG+GIIGSNGDF
Sbjct: 61
QYGKVFTVWMGPIPAVQICDFDVAHETHVKKAHTFGHRYTFGAMEYIREGKGIIGSNGDF 120
Query: 121
WLEHRRFALMTLRNFGVGRTIMEDKIMDEYRYRFKDFKRTHFKNGAIQVNASSIFDLLVG 180
WLEHRRFALMTLRNFG+GR I+EDKIM+EYRYRF+DFK+T+FK+GAIQVNASS+FDLLVG
Sbjct: 121
WLEHRRFALMTLRNFGLGRNIIEDKIMEEYRYRFEDFKKTNFKDGAIQVNASSLFDLLVG 180
Query: 181 SIINQLLVSERFEQDDQEFEKLKTSLAEALENISIIEGFLPLWVLKSPLMKWRTKITFAP
240
SIINQLLVSERFEQDD+EFE+LKT+LA ALEN SIIEG LPLW+LKS MKWRTK TFAP
Sbjct: 181
SIINQLLVSERFEQDDEEFEELKTNLAMALENGSIIEGVLPLWMLKSRFMKWRTKTTFAP 240
Query: 241 FDFIFELGNRGIQRRVAAIENGTHTLSEEGDDFVDAFIVKMEKDKKEGIDSSFTLETLAI
300
FDF+FE+G +GIQRRVAAIENGTHTLSEEGDDFVDAFIVKMEKDKK+GIDSSFTLETLA+
Sbjct: 241 FDFVFEVGKKGIQRRVAAIENGTHTLSEEGDDFVDAFIVKMEKDKKDGIDSSFTLETLAV
300
Query: 301
DLFDLWQAGQETTSTTLTWACVCLLNHPEVVEKLRKELTEVTGGTRGVSLTDRTKTPYLN 360
DLFDLWQAGQETTSTTLTWAC CLLNHPEVVEKLRKELTEVTGG RGVSLTDRTKTPYLN
Sbjct: 301
DLFDLWQAGQETTSTTLTWACACLLNHPEVVEKLRKELTEVTGGARGVSLTDRTKTPYLN 360
Query: 361
ANINEFQRIASILNVNLFRVLEEDTTIDSQPVPAGALVTTNLSMLHTDEEIFKNPQEFRP 420
A INE QRI+SILNVNL R+LEED
ID PVPAG TT L++LHTDEE FKN +EF P
Sbjct: 361
ATINEVQRISSILNVNLLRILEEDAVIDGHPVPAGTAFTTQLALLHTDEETFKNHKEFIP 420
Query: 421
ERFMENNNLEKRLIPFGIGKRACPGESLARAELFLITGNMILDYDLEPVGTLPKIETTTP 480
ERF+ENNNLEKRLIPFGIGKR+CPGESLA+AEL+LI GN+++D+DL+PVG +PKIE+ TP
Sbjct: 421
ERFLENNNLEKRLIPFGIGKRSCPGESLAKAELYLIIGNLVIDFDLKPVGAIPKIESPTP 480
Query: 481 FAPMKRPPVYDIRFVPRS 498
F+P+KRPPVYDIRF+ R+
Sbjct: 481 FSPVKRPPVYDIRFISRA 498
>CYP23A1
B0304.3 U39472 529 aa
(revised 12/8/2003)
Length = 529
Score = 2756 (970.2 bits), Expect =
2.4e-290, P = 2.4e-290
Identities = 529/534 (99%), Positives =
529/534 (99%)
Query: 1
MPSLRNAILLTIATTFLYFRIMRTLNFDNYMATYIYFLTCITLYAIYELNYKRRRLPNGP 60
MPSLRNAILLTIATTFLYFRIMRTLNFDNYMATYIYFLTCITLYAIYELNYKRRRLPNGP
Sbjct: 1
MPSLRNAILLTIATTFLYFRIMRTLNFDNYMATYIYFLTCITLYAIYELNYKRRRLPNGP 60
Query: 61
VPWLVAGNMPSFINVNNVDVLFQSWKQQYGGIFTVWIGPIPLVMVSDLPTIKKYFIQHAD 120
VPWLVAGNMPSFINVNNVDVLFQSWKQQYGGIFTVWIGPIPLVMVSDLPTIKKYFIQHAD
Sbjct: 61
VPWLVAGNMPSFINVNNVDVLFQSWKQQYGGIFTVWIGPIPLVMVSDLPTIKKYFIQHAD 120
Query: 121
SFSNRWRNFVTDSIMEGSNGIVQIDGNKWREQRRFALHTLRDFGVGKPLMEQMITLEVTS 180
SFSNRWRNFVTDSIMEGSNGIVQIDGNKWREQRRFALHTLRDFGVGKPLMEQMITLEVTS
Sbjct: 121
SFSNRWRNFVTDSIMEGSNGIVQIDGNKWREQRRFALHTLRDFGVGKPLMEQMITLEVTS 180
Query: 181
LMNHMEKSCGLDGKELHLCPSIAVCVGNIINNMLFGLRFNQDNSYMHRLHQLLDDQSHTV 240
LMNHMEKSCGLDGKELHLCPSIAVCVGNIINNMLFGLRFNQDNSYMHRLHQLLDDQSHTV
Sbjct: 181 LMNHMEKSCGLDGKELHLCPSIAVCVGNIINNMLFGLRFNQDNSYMHRLHQLLDDQSHTV
240
Query: 241
MQPIMGAYIAFPVTSKIPIINGEWNRLMGIKNELLEFLETQIEGHRMNWKDEMIEQEPED 300
MQPIMGAYIAFPVTSKIPIINGEWNRLMGIKNELLEFLETQIEGHRMNWKDEMIEQEPED
Sbjct: 241
MQPIMGAYIAFPVTSKIPIINGEWNRLMGIKNELLEFLETQIEGHRMNWKDEMIEQEPED 300
Query: 301
LTYAYMIEVEKRKRNGEDVGFFDDQQLKMLLLDLFFAGMETTVTTLKWAFLLMAKNQKVQ 360
LTYAYMIEVEKRKRNG
FDDQQLKMLLLDLFFAGMETTVTTLKWAFLLMAKNQKVQ
Sbjct: 301
LTYAYMIEVEKRKRNG-----FDDQQLKMLLLDLFFAGMETTVTTLKWAFLLMAKNQKVQ 355
Query: 361
KNVQAELDSIGQPMIEIQHRTRLPYVQATINEIQRIANILPINLLRTVAEDIEIDGYNFK 420
KNVQAELDSIGQPMIEIQHRTRLPYVQATINEIQRIANILPINLLRTVAEDIEIDGYNFK
Sbjct: 356
KNVQAELDSIGQPMIEIQHRTRLPYVQATINEIQRIANILPINLLRTVAEDIEIDGYNFK 415
Query: 421 KGDLIIPQISILMNDPEIFENPEEFNPSRFLDEDNNVKKIDEFLPFSIGRRQCLGESLAR
480
KGDLIIPQISILMNDPEIFENPEEFNPSRFLDEDNNVKKIDEFLPFSIGRRQCLGESLAR
Sbjct: 416
KGDLIIPQISILMNDPEIFENPEEFNPSRFLDEDNNVKKIDEFLPFSIGRRQCLGESLAR 475
Query: 481 AELYLVFANLIQNFNFEVADDVTTERVLGLTVSPVEYSCKITRRGLDHNQNSVK
534
AELYLVFANLIQNFNFEVADDVTTERVLGLTVSPVEYSCKITRRGLDHNQNSVK
Sbjct: 476
AELYLVFANLIQNFNFEVADDVTTERVLGLTVSPVEYSCKITRRGLDHNQNSVK 529
Compare to the sequence below:
>cb25.fpc3052
ACC=CAAC01000064 86% to CYP23A1
Length = 534
Score = 2430 (855.4 bits), Expect =
8.4e-256, P = 8.4e-256
Identities = 454/534 (85%), Positives =
493/534 (92%)
Query: 1
MPSLRNAILLTIATTFLYFRIMRTLNFDNYMATYIYFLTCITLYAIYELNYKRRRLPNGP 60
MPS++ AILL++ T+FLYFRIM++
D+Y TYIY C Y YE
NYKRRRLPNGP
Sbjct: 1
MPSIKYAILLSVVTSFLYFRIMKSFEMDSYTTTYIYLFFCTISYIFYESNYKRRRLPNGP 60
Query: 61
VPWLVAGNMPSFINVNNVDVLFQSWKQQYGGIFTVWIGPIPLVMVSDLPTIKKYFIQHAD 120
+PWLVAGNMPSFINV NVD LF
WKQ+YGGIFTVWIGPIPLVMVSDLPTIKKYFIQHAD
Sbjct: 61
MPWLVAGNMPSFINVKNVDDLFLYWKQRYGGIFTVWIGPIPLVMVSDLPTIKKYFIQHAD 120
Query: 121
SFSNRWRNFVTDSIMEGSNGIVQIDGNKWREQRRFALHTLRDFGVGKPLMEQMITLEVTS 180
+FSNRWRNFVTDSIMEGSNGIVQIDG+KWREQRRFALHTLRDFGVG+PLMEQMITLEVT+
Sbjct: 121 AFSNRWRNFVTDSIMEGSNGIVQIDGDKWREQRRFALHTLRDFGVGRPLMEQMITLEVTT
180
Query: 181
LMNHMEKSCGLDGKELHLCPSIAVCVGNIINNMLFGLRFNQDNSYMHRLHQLLDDQSHTV 240
LMNHM KSCGL
KE++LCPSIAVCVGNIINNMLFGLRFNQDNSYMHRLH LLDDQSHTV
Sbjct: 181
LMNHMAKSCGLSTKEVNLCPSIAVCVGNIINNMLFGLRFNQDNSYMHRLHSLLDDQSHTV 240
Query: 241
MQPIMGAYIAFPVTSKIPIINGEWNRLMGIKNELLEFLETQIEGHRMNWKDEMIEQEPED 300
MQPIMGAYIAFPVT+K+P INGEWNRLMGIK ELLEFLE QI+ HR NWK+EM+EQEPED
Sbjct: 241
MQPIMGAYIAFPVTTKVPFINGEWNRLMGIKKELLEFLEGQIQKHRENWKEEMMEQEPED 300
Query: 301 LTYAYMIEVEKRKRNGEDVGFFDDQQLKMLLLDLFFAGMETTVTTLKWAFLLMAKNQKVQ
360
LTYAYMIEVEKRKR GEDVGFFDDQQLKMLLLDLFFAGMETTVTTLKWAFLLM+KN
+VQ
Sbjct: 301 LTYAYMIEVEKRKRAGEDVGFFDDQQLKMLLLDLFFAGMETTVTTLKWAFLLMSKNPRVQ
360
Query: 361 KNVQAELDSIGQPMIEIQHRTRLPYVQATINEIQRIANILPINLLRTVAEDIEIDGYNFK
420
+ VQ ELDSI QPM+EIQHRTRLPY+QATINEIQRIANILPINLLRTVAEDI+IDGY FK
Sbjct: 361
RKVQEELDSIAQPMVEIQHRTRLPYIQATINEIQRIANILPINLLRTVAEDIDIDGYQFK 420
Query: 421 KGDLIIPQISILMNDPEIFENPEEFNPSRFLDEDNNVKKIDEFLPFSIGRRQCLGESLAR
480
KGDL IPQISILMNDPEIF+NP++F P RFLDE+ NVKKIDEFLPFSIGRRQCLGESLAR
Sbjct: 421
KGDLTIPQISILMNDPEIFKNPKDFCPERFLDENLNVKKIDEFLPFSIGRRQCLGESLAR 480
Query: 481
AELYLVFANLIQNFNFEVADDVTTERVLGLTVSPVEYSCKITRRGLDHNQNSVK 534
AELYL+FANL+QNF FEV++DVTTERVLGLTVSPV+Y+CKI+RRGLDHN+N VK
Sbjct: 481
AELYLIFANLMQNFKFEVSEDVTTERVLGLTVSPVQYTCKISRRGLDHNENCVK 534
>CYP29A4
B0331 500 aa 4 small changes,
probably errors in my old sequence
Length = 500
Score = 2532 (891.3 bits), Expect =
1.3e-266, P = 1.3e-266
Identities = 481/486 (98%), Positives =
481/486 (98%)
Query: 1
MSILIPVALALLFVYLLSFYDTIRLMRKFWIYGGKMPGPPAHPIFGNASLFKNKTTKDFV 60
MSILIPVALALLFVYLLSFYDTIRLMRKFWIYGGKMPGPPAHPIFGNASLFKNKT DFV
Sbjct: 1
MSILIPVALALLFVYLLSFYDTIRLMRKFWIYGGKMPGPPAHPIFGNASLFKNKTXSDFV 60
Query: 61
ELFVQLAHEARSKGANLMRTQVMNRIYVWPLNGKTAATILESSTEVNKGDDYAFLVPWLG 120
ELFVQLAHEARSKGANLMRTQVMNRIYVWPLNGKTAATILESSTEVNKGDDYAFLVPWLG
Sbjct: 61 ELFVQLAHEARSKGANLMRTQVMNRIYVWPLNGKTAATILESSTEVNKGDDYAFLVPWLG
120
Query: 121
GGLLMEKGEKWKSHRRILTPAFHFAKLEGYLDVFNSESKILIDCLEKIAETQETVDLFPF 180
GGLLMEKGEKWKSHRRILTPAFHFAKLEGYLDVFNSES ILIDCLEKIAETQETVDLFPF
Sbjct: 121
GGLLMEKGEKWKSHRRILTPAFHFAKLEGYLDVFNSES-ILIDCLEKIAETQETVDLFPF 179
Query: 181
FKRCTLDIICGTAMGIKLDAQNVHNLGYVQAVEGFNKLTVEYSLNPFLWNRFVYWALGYQ 240
FKRCTLDIICGTAMGIKLDAQNVHNLGYVQAVEGFNKLTV YSLNPFLWNRFVYWALGYQ
Sbjct: 180
FKRCTLDIICGTAMGIKLDAQNVHNLGYVQAVEGFNKLTVSYSLNPFLWNRFVYWALGYQ 239
Query: 241
KMHDDFLYTLKKFTNDAIVERRTVIASGEIEKETSKRKMNFLDILLNSEESNELTSDEIR 300
KMHDDFLYTLKKFTNDAIVERRTVIASGEIEKETSKRKMNFLDILLNSEESNELTSDEIR
Sbjct: 240
KMHDDFLYTLKKFTNDAIVERRTVIASGEIEKETSKRKMNFLDILLNSEESNELTSDEIR 299
Query: 301 KEVDTFMFAGHDTTSTSLSWLCWNIAHNPEVQENVYKEIISIFGEDPNQDVTSENINRLE
360
KEVDTFMFAGHDTTSTSLSWLCWNIAHNPEVQENVYKEIISIFGEDPNQDVTSENINRLE
Sbjct: 300
KEVDTFMFAGHDTTSTSLSWLCWNIAHNPEVQENVYKEIISIFGEDPNQDVTSENINRLE 359
Query: 361 YTERVLKESKRMFPPVPGFQRKLTKDIVIDGITIPSEGNITISPTVLHCNPFVYQNPEKF
420
YTERVLKESKRMFPPVPGFQRKLTKDIVI GITIPSEGNITISPTVLHCNPFVYQNPEKF
Sbjct: 360 YTERVLKESKRMFPPVPGFQRKLTKDIVIGGITIPSEGNITISPTVLHCNPFVYQNPEKF
419
Query: 421
DPDRFLPEECAKRHSYDYIPFSAGLRNCIGQKFSILNEKVMLIHILRNFKLEPKLEFYET 480
DPDRFLPEECAKRHSYDYIPFSAGLRNCIGQKFSILNEKVMLIHILRNFKLEPKLEFYET
Sbjct: 420
DPDRFLPEECAKRHSYDYIPFSAGLRNCIGQKFSILNEKVMLIHILRNFKLEPKLEFYET 479
Query: 481 KPLFEV 486
KPLFEV
Sbjct: 480 KPLFEV 485
C03G6.14;
CE07888. This gene is badly
misassembled
MFLVLIFLALSCWLIIRQYQKVSRLPPGP
skipped sequence here
KYGNIFTLWVGPVPHVSICDY 50
ETSHEVFVKGANKYADIAHAPLFRELRQEMGVLVTNGSHWSTMKRFALHT
100
FRDMGVGKDLMETRIMEELDARCADTDKSATDGVTVAQAGDFFDLTVGSI
150
INSILVGKRFEEHNKDDFLKIKEAMGAAFEVFSPFDMAVPVWFLRTFFRS
200
RYDMMMTTQNTAKRFAAAEAVK
skipped seq here
SIETLKTMIIDLWMTGQETTTTTLISGF
250
TQLLLHPEVMVKAREEILKITENGSRHLSLTDRTSTPYLNAMIGEIQRHA
300
SILNVSFWKINKELTYMGGHPVDAGALVTAQLSALHVNDTIFKNPQEFDP
350
ERFIRDEELLQKVIPFGVGKRSCIGESLARAELYL
C-term is wrong seq, but my C-term may also be wrong
ISLLCICGTSNKSFLHKYLPLWNSVALLLPVKLSRPAFAALISSLPLDKLLPHI
>C03G6.14 C13B3.B CYP35A1 502 aa
yellow is
at an intron boundary
MFLVLIFLALSCWLIIRQYQKVSRLPPGPVSFPIIGNLPHIIYYLWATGGIVS
TLDLFRKKYGNIFTLWVGPVPHVSICDYETSHEVFVKGANKYADIAHAPLFR
ELRRW*MGVLVTNGSHWSTMKRFALHTFRDMGVGKDLMETRIMEELDARC
ADTDKSATDGVTVAQAGDFFDLTVGSIINSILVGKRFEEHNKDDFLKIKEAMG
AAFEVFSPFDMAVPVWFLRTFFRSRYDMMMTTQNTAKRFAAAEAVKRIED
IKSGAYEIDESNIEDYTDAFLLKIQKDGEDLDFNIETLKTMIIDLWMTGQETTTT
TLISGFTQLLLHPEVMVKAREEILKITENGSRHLSLTDRTSTPYLNAMIGEIQRHASI
LNVSFWKINKELTYMGGHPVDAGALVTAQLSALHVNDTIFKNPQEFDPERFIRDEE
LLQKVIPFGVGKRSCIGESLARAELYL
VRHLQYRISNFLFRLLGTFCSAINSNRMEH
CRQQSCCRIAPEKDHSSWK*
18318
IIGNLLLRYKFEPHGTLSTTELLPYSAGKRPFKLEMKFVKI 18196 correct C-term
Compare my
35A1 assembly to 35A2 below
>CYP35A2
C03G6.15 C13B3.A 495
aa
Length = 496
Score = 2005 (705.8 bits), Expect =
9.2e-211, P = 9.2e-211
Identities = 386/505 (76%), Positives =
431/505 (85%)
Query: 1
MFLVLIFLALSCWLIIRQYQKVSRLPPGPVSFPIIGNLPHIIYYLWATGGIVSTLDLFRK
60
MF VL F L +LI+RQYQKVSRLPPGP+S P+IGNLP IIYYLW+TGGIVSTLDLFRK
Sbjct: 1
MFFVLFFSVLLGYLIVRQYQKVSRLPPGPISLPLIGNLPQIIYYLWSTGGIVSTLDLFRK
60
Query: 61
KYGNIFTLWVGPVPHVSICDYETSHEVFVKGANKYADIAHAPLFRELRRW*MGVLVTNGS 120
+YGNIFTLWVGP+PHVSI
DYETSHEVFVK A KYAD HAP+ R++R +GVL+TNG
Sbjct: 61
RYGNIFTLWVGPIPHVSIADYETSHEVFVKNAGKYADKFHAPVMRDVRSK-IGVLITNGD 119
Query: 121
HWSTMKRFALHTFRDMGVGKDLMETRIMEELDARCADTDKSATDGVTVAQAGDFFDLTVG 180
HW M+RF+L FR+MGVGKD+METRIMEELDARC+D DK
AT+GVT+ A +FFDLTVG
Sbjct: 120
HWQEMRRFSLQAFRNMGVGKDIMETRIMEELDARCSDIDKLATNGVTITHASEFFDLTVG 179
Query: 181
SIINSILVGKRFEEHNKDDFLKIKEAMGAAFEVFSPFDMAVPVWFLRTFFRSRYDMMMTT 240
SIINSILVGKRFEE K +FLKIKE M
A+FE FSPFDM PVWFL+TFF+ RYD + +
Sbjct: 180
SIINSILVGKRFEEDTKHEFLKIKETMDASFETFSPFDMTAPVWFLKTFFKHRYDKIWSA 239
Query: 241 QNTAKRFAAAEAVKRIEDIKSGAYEIDESNIEDYTDAFLLKIQKDGEDLDFNIETLKTM
300
Q TAK FAAAEA+KR+E IKSG
Y IDE+N++DYTDAFLLKIQK+GE DFNIETLKTM
Sbjct: 240 QETAKNFAAAEAIKRVESIKSGKYVIDENNLQDYTDAFLLKIQKEGESKDFNIETLKTM
298
Query: 301
IIDLWMTGQETTTTTLISGFTQLLLHPEVMVKAREEILKITENGSRHLSLTDRTSTPYLN 360
IIDLWMTGQETTTTTLISGF QLLLHPEVM+KAREEILKITENGSRHLSLTDRTSTPY+N
Sbjct: 299 IIDLWMTGQETTTTTLISGFNQLLLHPEVMIKAREEILKITENGSRHLSLTDRTSTPYVN
358
Query: 361
AMIGEIQRHASILNVSFWKINKELTYMGGHPVDAGALVTAQLSALHVNDTIFKNPQEFDP 420
A+IGEIQRHASILNVSFWKINKE TYMGGHPVDAGALVT+QLSALHVN+T+FKNPQEF+P
Sbjct: 359
AVIGEIQRHASILNVSFWKINKEFTYMGGHPVDAGALVTSQLSALHVNETVFKNPQEFNP 418
Query: 421
ERFIRDEELLQKVIPFGVGKRSCIGESLARAELYLVRHLQYRISNFLFRLLGTFCSAINS 480
ERFIRD +LLQKVIPFGVGKR+C+GESLA+AELYLVR + YR S TF A NS
Sbjct: 419
ERFIRDGKLLQKVIPFGVGKRNCLGESLAKAELYLVRFVFYRSSV-------TFSFATNS 471
Query: 481 NRMEHCRQQSCCRIAPEKDHSSWK* 505
N M + S C A EKDHSSWK*
Sbjct: 472 NNMANYPLPSLCHTALEKDHSSWK* 496
>CYP35A2
C03G6.15 C13B3.A 495
aa
Length = 496
Score = 2342 (824.4 bits), Expect =
1.8e-246, P = 1.8e-246
Identities = 451/454 (99%), Positives =
453/454 (99%)
My C-term
may be wrong see below. By
comparison to the briggsae seqs
It looks
like all my C-term exons for elegans 35As are wrong. They may be in an alternative reading frame from the correct
sequences.
Query: 1
MFFVLFFSVLLGYLIVRQYQKVSRLPPGPISLPLIGNLPQIIYYLWSTGGIVSTLDLFRK 60
MFFVLFFSVLLGYLIVRQYQKVSRLPPGPISLPLIGNLPQIIYYLWSTGGIVSTLDLFRK
Sbjct: 1
MFFVLFFSVLLGYLIVRQYQKVSRLPPGPISLPLIGNLPQIIYYLWSTGGIVSTLDLFRK 60
Query: 61 RYGNIFTLWVGPIPHVSIADYETSHEVFVKNAGKYADKFHAPVMRDVRNDIGVLITNGDH 120
RYGNIFTLWVGPIPHVSIADYETSHEVFVKNAGKYADKFHAPVMRDVR+ IGVLITNGDH
Sbjct: 61
RYGNIFTLWVGPIPHVSIADYETSHEVFVKNAGKYADKFHAPVMRDVRSKIGVLITNGDH 120
Query: 121
WQEMRRFSLQAFRNMGVGKDIMETRIMEELDARCSDIDKLATNGVTITHASEFFDLTVGS 180
WQEMRRFSLQAFRNMGVGKDIMETRIMEELDARCSDIDKLATNGVTITHASEFFDLTVGS
Sbjct: 121
WQEMRRFSLQAFRNMGVGKDIMETRIMEELDARCSDIDKLATNGVTITHASEFFDLTVGS 180
Query: 181
IINSILVGKRFEEDTKHEFLKIKETMDASFETFSPFDMTAPVWFLKTFFKHRYDKIWSAQ 240
IINSILVGKRFEEDTKHEFLKIKETMDASFETFSPFDMTAPVWFLKTFFKHRYDKIWSAQ
Sbjct: 181
IINSILVGKRFEEDTKHEFLKIKETMDASFETFSPFDMTAPVWFLKTFFKHRYDKIWSAQ 240
Query: 241
ETAKNFAAAEAIKRVESIKSGKYVIDENNLQDYTDAFLLKIQKEGESKDFNIETLKTMII 300
ETAKNFAAAEAIKRVESIKSGKYVIDENNLQDYTDAFLLKIQKEGESKDFNIETLKTMII
Sbjct: 241
ETAKNFAAAEAIKRVESIKSGKYVIDENNLQDYTDAFLLKIQKEGESKDFNIETLKTMII 300
Query: 301
DLWMTGQETTTTTLISGFNQLLLHPEVMIKAREEILKITENGSRHLSLTDRTSTPYVNAV 360
DLWMTGQETTTTTLISGFNQLLLHPEVMIKAREEILKITENGSRHLSLTDRTSTPYVNAV
Sbjct: 301
DLWMTGQETTTTTLISGFNQLLLHPEVMIKAREEILKITENGSRHLSLTDRTSTPYVNAV 360
Query: 361
IGEIQRHASILNVSFWKINKEFTYMGGHPVDAGALVTSQLSALHVNETVFKNPQEFNPER 420
IGEIQRHASILNVSFWKINKEFTYMGGHPVDAGALVTSQLSALHVNETVFKNPQEFNPER
Sbjct: 361
IGEIQRHASILNVSFWKINKEFTYMGGHPVDAGALVTSQLSALHVNETVFKNPQEFNPER 420
Query: 421
FIRDGKLLQKVIPFGVGKRNCLGESLAKAELYLI 454
FIRDGKLLQKVIPFGVGKRNCLGESLAKAELYL+
Sbjct: 421
FIRDGKLLQKVIPFGVGKRNCLGESLAKAELYLV 454
>C03G6.15 C13B3.A CYP35A2 495 aa
yellow is
at intron boundary
MFFVLFFSVLLGYLIVRQYQKVSRLPPGPISLPLIGNLPQIIYYLWSTGGIVST
LDLFRKRYGNIFTLWVGPIPHVSIADYETSHEVFVKNAGKYADKFHAPVMR
DVRSKIGVLITNGDHWQEMRRFSLQAFRNMGVGKDIMETRIMEELDARCSD
IDKLATNGVTITHASEFFDLTVGSIINSILVGKRFEEDTKHEFLKIKETMDASF
ETFSPFDMTAPVWFLKTFFKHRYDKIWSAQETAKNFAAAEAIKRVESIKSGKYVID
ENNLQDYTDAFLLKIQKEGESKDFNIETLKTMIIDLWMTGQETTTTTLISGFNQL
LLHPEVMIKAREEILKITENGSRHLSLTDRTSTPYVNAVIGEIQRHASILNVSFWKIN
KEFTYMGGHPVDAGALVTSQLSALHVNETVFKNPQEFNPERFIRDGKLLQKVIPF
GVGKRNCLGESLAKAELYL
VRFVFYRSSVTFSFATNSNNMANYPLPSLCHTALEKDHSSWK*
Probable
correct C-term =
IFGNLLLRYKFEQHGKLSTTELMPYSAGKRPFKLEMKFVKI*
cDNA
evidence
>gi|30742705|gb|CB400978.1|CB400978 UniGene info OSTF185G5_1 AD-wrmcDNA
Caenorhabditis elegans cDNA.
Length = 544
Score = 210 bits (534), Expect = 7e-56
Identities = 100/102 (98%), Positives =
101/102 (99%)
Frame = +3
Query:
1
LDLFRKRYGNIFTLWVGPIPHVSIADYETSHEVFVKNAGKYADKFHAPVMRDVRSKIGVL 60
LDLFRKRYGNIFTLWVGPIPHVSIADYETSHEVFVKNAGKYADKFHAPVMRDVR+ IGVL
Sbjct: 135
LDLFRKRYGNIFTLWVGPIPHVSIADYETSHEVFVKNAGKYADKFHAPVMRDVRNDIGVL 314
Query:
61
ITNGDHWQEMRRFSLQAFRNMGVGKDIMETRIMEELDARCSD 102
ITNGDHWQEMRRFSLQAFRNMGVGKDIMETRIMEELDARCSD
Sbjct: 315
ITNGDHWQEMRRFSLQAFRNMGVGKDIMETRIMEELDARCSD 440
>CYP35A.e
cb25.fpc0023 76% to CYP35A.b briggsae seq on bottom
Length = 495
Score = 2054 (723.0 bits), Expect =
5.9e-216, P = 5.9e-216
Identities = 379/494 (76%), Positives =
445/494 (90%)
Query: 1
MFFVLFFSVLLGYLIVRQYQKVSRLPPGPISLPLIGNLPQIIYYLWSTGGIVSTLDLFRK 60
MFFVL L
+L+VRQYQKVSRLPPGP+SLPLIGNLPQI+YYL++TGG+VSTLD FRK
Sbjct: 1
MFFVLIVFTFLTWLVVRQYQKVSRLPPGPVSLPLIGNLPQIVYYLYTTGGVVSTLDFFRK 60
Query: 61
RYGNIFTLWVGPIPHVSIADYETSHEVFVKNAGKYADKFHAPVMRDVRNDIGVLITNGDH 120
RYGNIFTLWVGPIPHVSIADYETSHEVFVKNA KYADKFH+P+ R++R+ G+L NGDH
Sbjct: 61
RYGNIFTLWVGPIPHVSIADYETSHEVFVKNANKYADKFHSPIFREMRSKRGILTANGDH 120
Query: 121
WQEMRRFSLQAFRNMGVGKDIMETRIMEELDARCSDIDKLATNGVTITHASEFFDLTVGS 180
WQEMRRF+L
FRNMGVGKD+METRIMEEL+ARC+DIDK A NGVT T A+EFFDLTVGS
Sbjct: 121
WQEMRRFALFTFRNMGVGKDLMETRIMEELNARCADIDKAAVNGVTTTQAAEFFDLTVGS 180
Query: 181 IINSILVGKRFEEDTKHEFLKIKETMDASFETFSPFDMTAPVWFLKTFFKHRYDKIWSAQ
240
IINSILVGKRFEE K++FLKIKE
MDASFETFSPFDMT PVW LK FF R++K+ + Q
Sbjct: 181
IINSILVGKRFEEHNKNDFLKIKEVMDASFETFSPFDMTMPVWILKNFFPRRFEKMRNGQ 240
Query: 241 ETAKNFAAAEAIKRVESIKSGKYVIDENNLQDYTDAFLLKIQKEGESKDFNIETLKTMII
300
E++K FAA EA+KR++ IK+G+YVIDENN QDYTDAFL K+QK+GE++D+ +E+LKTMI+
Sbjct: 241
ESSKQFAAKEALKRIDEIKAGRYVIDENNFQDYTDAFLWKMQKDGENEDYKVESLKTMIL 300
Query: 301
DLWMTGQETTTTTLISGFNQLLLHPEVMIKAREEILKITENGSRHLSLTDRTSTPYVNAV 360
DLW+TGQETTTTTLISGFNQLLLH + M KAR E+ +ITENGSR++SL+DR TPY+NAV
Sbjct: 301
DLWITGQETTTTTLISGFNQLLLHSKFMEKARAELFEITENGSRNVSLSDRPKTPYLNAV 360
Query: 361
IGEIQRHASILNVSFWKINKEFTYMGGHPVDAGALVTSQLSALHVNETVFKNPQEFNPER 420
IGEIQRHASILNV+FW+ N E TYMGGH
VD+GALVT+QLSALHVNETVF++P++F+PER
Sbjct: 361
IGEIQRHASILNVNFWRWNNEPTYMGGHMVDSGALVTAQLSALHVNETVFEHPEKFDPER 420
Query: 421
FIRDGKLLQKVIPFGVGKRNCLGESLAKAELYLIFGNLLLRYKFEQHGKLSTTELMPYSA 480
FIRD KLLQKVIPFG+GKR+CLGESLA++ELYLIFGNLLLRYKF+ HG+LST E+MPYSA
Sbjct: 421
FIRDEKLLQKVIPFGLGKRSCLGESLARSELYLIFGNLLLRYKFQPHGELSTREIMPYSA 480
Query: 481 GKRPFKLEMKFVKI 494
GKRPFKLEM+F+K+
Sbjct: 481 GKRPFKLEMQFIKV 494
C03G6.15;
CE35217. = correct seq
MFFVLFFSVLLGYLIVRQYQKVSRLPPGPISLPLIGNLPQIIYYLWSTGG 50
IVSTLDLFRKRYGNIFTLWVGPIPHVSIADYETSHEVFVKNAGKYADKFH 100
APVMRDVRNDIGVLITNGDHWQEMRRFSLQAFRNMGVGKDIMETRIMEEL 150
DARCSDIDKLATNGVTITHASEFFDLTVGSIINSILVGKRFEEDTKHEFL 200
KIKETMDASFETFSPFDMTAPVWFLKTFFKHRYDKIWSAQETAKNFAAAE 250
AIKRVESIKSGKYVIDENNLQDYTDAFLLKIQKEGESKDFNIETLKTMII 300
DLWMTGQETTTTTLISGFNQLLLHPEVMIKAREEILKITENGSRHLSLTD 350
RTSTPYVNAVIGEIQRHASILNVSFWKINKEFTYMGGHPVDAGALVTSQL 400
SALHVNETVFKNPQEFNPERFIRDGKLLQKVIPFGVGKRNCLGESLAKAE 450
LYL IFGNLLLRYKFEQHGKLSTTELMPYSAGKRPFKLEMKFVKI
>CYP33B1
C25E10.2 U50311 495 aa
Length = 496
Score = 2536 (892.7 bits), Expect =
4.9e-267, P = 4.9e-267
Identities = 485/496 (97%), Positives =
489/496 (98%)
My seq
wrong at green region yellow is intron boundary
Query: 1
MILIFFALCTLFFLIHQYLWRRRGLPPGPTPIPIFGNLFQLSGSEAPGISIFQKWKDQYG 60
MILIFFALCTLFFLIHQYLWRRRGLPPGPTPIPIFGNLFQLSGSEAPGISIFQKWKDQYG
Sbjct: 1
MILIFFALCTLFFLIHQYLWRRRGLPPGPTPIPIFGNLFQLSGSEAPGISIFQKWKDQYG 60
Query: 61 PIFTFYMGPVPFVVLTDYQDIKETVIKDGDTYADKYLSPEFNKYFRGGEYGIMDISGDRW 120
PIFTFYMG PFVVLTDYQDIKETVIKDGDTYADKYLSPEFNKYFR ++ + +SGDRW
Sbjct: 61 PIFTFYMGIAPFVVLTDYQDIKETVIKDGDTYADKYLSPEFNKYFREMQFTKL-LSGDRW 119
Query: 121
KEHRKFAVLQLRELGVGKPLMESKILIEAEELIKKLKTAEILNEDFLLQSELDVAVGSVI 180
KEHRKFAVLQLRELGVGKPLMESKILIEAEELIKKLKTAEILNEDFLLQSELDVAVGSVI
Sbjct: 120
KEHRKFAVLQLRELGVGKPLMESKILIEAEELIKKLKTAEILNEDFLLQSELDVAVGSVI 179
Query: 181
NQFLFGYRFDRSKLFEFTRIKTLVNNFMEEVGKPLGVLAFTCHGIPSFLVKLMVSGIEEQ 240
NQFLFGYRFDRSKLFEFTRIKTLVNNFMEEVGKPLGVLAFTCHGIPSFLVKLMVSGIEEQ
Sbjct: 180
NQFLFGYRFDRSKLFEFTRIKTLVNNFMEEVGKPLGVLAFTCHGIPSFLVKLMVSGIEEQ 239
Query: 241
KRELFRFLRKQIDGAKSQINYEEEHNEDFVEAYLRKKFQREQKNDFDSYCDSQLENVCFD 300
KRELFRFLRKQIDGAKSQINYEEEHNEDFVEAYLRKKFQREQKNDFDSYCDSQLENVCFD
Sbjct: 240
KRELFRFLRKQIDGAKSQINYEEEHNEDFVEAYLRKKFQREQKNDFDSYCDSQLENVCFD 299
Query: 301
IWAAGFDTLTNTVGFLIAYAINYPEMQMLIHQEIDNYLAHHSRLLTLADKNALVYFNAFA 360
IWAAGFDTLTNTVGFLIAYAINYPEMQMLIHQEIDNYLAHHSRLLTLADKNALVYFNAFA
Sbjct: 300
IWAAGFDTLTNTVGFLIAYAINYPEMQMLIHQEIDNYLAHHSRLLTLADKNALVYFNAFA 359
Query: 361
NEAQRVSNILPMNLPHALTRDVKLKGYHLKKGTGVIHQIANVMTDETIFKDSQRFDPNRF 420
NEAQRVSNILPMNLPHALTRDVKLKGYHLKKGTGVIHQIANVMTDETIFKDSQRFDPNRF
Sbjct: 360
NEAQRVSNILPMNLPHALTRDVKLKGYHLKKGTGVIHQIANVMTDETIFKDSQRFDPNRF 419
Query: 421
IDENGKLKKIEELCPFSMGKRQCIGEGLARMEIFLLAANLFNYFEFLPASDGLPSLYKDF 480
IDENGKLKKIEELCPFSMGKRQCIGEGLARMEIFLLAANLFNYFEFLPASDGLPSLYKDF
Sbjct: 420 IDENGKLKKIEELCPFSMGKRQCIGEGLARMEIFLLAANLFNYFEFLPASDGLPSLYKDF
479
Query: 481 SLVSHVIPYKCRQYIP 496
SLVSHVIPYKCRQYIP
Sbjct: 480 SLVSHVIPYKCRQYIP 495
>CYP32A1 C26F1.2 U53148 508 aa also Y97E10