P450s that have appeared since the 1993 P450 nomenclature update.
Part C covering CYP10 to CYP69
Animal P450 names continue at CYP301 in this file
Lower eukaryote names continue at CYP501 in this file
ALL Drosophila melanogaster P450S ARE NOW INCLUDED
This includes list references that were incomplete and duplications of
sequences that were already in the update. If a sequence is assigned an
accession number that was not in the old update it is included in this list.
Some expressed sequence tags (ESTs) are also included from humans.
This list was last revised on Dec. 31, 2007.
Added all Neurospora P450s see 527A1-553A1
Added CYP51 Trypanosoma brucei
Added Fugu P450s, added CYP505A3 added CYP18 Spodoptera
Added all human genes and pseudogenes
Added 104 Anopheles genes
Revised May 13, 2003 named all remaining Dictyostelium P450 genes
Many sequences have been added without updating this list (May 11, 2005)
Added names for Nectria haematococca (158 genes/pseudogenes) Feb. 16, 2006
Added all Phanerochaete chrysosporium (white rot fungi) genes Aug. 29, 2007
Compiled by David R. Nelson
There are 80 C. elegans P450s listed here, surpassing the known mouse and human
complements. The C. elegans genome is now about 90% finished sequence and 9%
unfinished (one strand or one chemistry). About 1Mb is not cloned at three
telomeres and two internal sites. A special issue of Science appeared in Dec. 98 even though the genome is not completely done. The amount of sequence in the Blast searchable database at Washington Univ. is 140Mb, more than the 100Mb size of the genome. Therefore, we can guess that this set includes all the P450 genes in C. elegans, but the distrubution is not even. Most P450 genes (43 genes) are on chromosome V. see additional info on C. elegans P450s see this list. To see the actual sequences go to the C. elegans sequence file. So far we are missing CYP11A, CYP11B, CYP17, CYP19, CYP21, CYP24 and CYP27A and CYP27B. Does C. elegans make steroids? The present evidence would suggest not. Does C. elegans have
mitochondrial P450s? There is one probable mitochondrial P450 in C. elegans on
cosmid ZK177 named CYP44. It was thought to be incomplete but now a full length sequence
has been assembled from the genomic sequence.
10A Subfamily
11A Subfamily
11B Subfamily
12A Subfamily
12B Subfamily
13A Subfamily
13B Subfamily
14A Subfamily
16A Subfamily
17A Subfamily
18A Subfamily
19A Subfamily
21A Subfamily
22A Subfamily
23A Subfamily
24A Subfamily
25A Subfamily
26A Subfamily
27A Subfamily
27B Subfamily
28A Subfamily
29A Subfamily
30A Subfamily
31A Subfamily
32A Subfamily
33A Subfamily
33B Subfamily
33C Subfamily
33D Subfamily
33E Subfamily
34A Subfamily
35A Subfamily
35B Subfamily
35C Subfamily
35D Subfamily
36A Subfamily
37A Subfamily
37B Subfamily
40A Subfamily
41A Subfamily
42A Subfamily
43A Subfamily
44A Subfamily
45A Subfamily
46A Subfamily
51A Subfamily
52A Subfamily
52B Subfamily
52C Subfamily
52D Subfamily
52E Subfamily
52F Subfamily
53A Subfamily
53B Subfamily
54A Subfamily
55A Subfamily
56A Subfamily
57A Subfamily
58A Subfamily
59A Subfamily
60A Subfamily
60B Subfamily
61A Subfamily
62A Subfamily
63A Subfamily
64A Subfamily
65A Subfamily
66A Subfamily
67A Subfamily
10A Subfamily
CYP10A1 Lymnaea stagnalis (pond snail)
GenEMBL S46130 (1870bp) PIR JX0225 (545 amino acids)
AAB23599.1
Teunissen,Y., Geraerts,W.P., van Heerikhuizen,H., Planta,R.J.
and Joosse,J.
Molecular cloning of a cDNA encoding a member of a novel
cytochrome P450 family in the mollusc Lymnaea stagnalis.
J. Biochem. 112, 249-252 (1992)
Mitochondrial clan
1 MAIMKKFIHH SLKQLIKPNL TSTKRVVSTS PRKEQGVAAI SLEPSEMAQC PFRKSIDTFT
61 ETTNAVKAPG MTEVQPFERI PGPKGLPIVG TLFDYFKKDG PKFSKMFEVY RQRALEFGNI
121 YYEKVGHFHC VVISSPGEYS RLVHAERQYP NRREMVPIAY YRKQKGFDLG VVNSQGEEWY
181 RQRTVVSKKM LKLAEVSNFS TQMGEVSDDF VKRLSHVRDS HGEIPALERE LFKWAMESIG
241 TFLFEERIGC LGQETSPMAQ TFIANLEGFF KTLQPLMYNL PTYKLWSTKL WKQFENYSDN
301 VIDIGRSLVE KKWHPCKMEV TQNLHLISYL VNNGSMSTKE VTGLIVDLML AAVETTSSAT
361 VWCLYNLAKN PQVQEKLFQE ITEAQAKNNG TISAEDLCKL PMVKAVVKET LRLYPITYST
421 SRNIAEDMEL GGYTIPAGTH VQANLYGMYR DPSLFPEPEG ILPERWLRMN GSQMDATIKS
481 TSQLVWGHGA RMCLGRRIAE QEMHITLSKI IQNFTLSYNH DDVEPILNTM LTPDRPVRIE
541 FKPRQ
CYP10A2 Biomphalaria glabrata (bloodfluke planorb, carries schistosomiasis)
ESTs EV821686, EV821681, EV817279
Probable mitochondrial P450
LFDYFKKDGPRFNKI
FQVQQQRSAQYGEIYYEKIADFESVIESSPEEYNRLERSEGKYPCRREMA
PMAYYNRKKGYALGIVNSQGKDWYNLRTVVSKKMLKLSEVS
YGMFHNPDYFPEPETFLPERWLKENKMDATIKSASQLVWGHGARMCLGRRLAEQELHI
TIAKIIENFQLNYHGDEVQPILNTTMVPDRPVNIQFSRRQISKDTVFNNTH*
CYP10A3 Lottia gigantea (owl limpet)
JGI Protein ID:233081
53% to CYP10A1
MSSLIPKTFAKIAVTALPEIATKRVVSTSVAHKQSARVIEVDPLTLGTC
PFRSTFEKVESIVNPIEPKVEPQELTKETGIKSFEHVPGPK
GLPILGSLLDYFKKDGLRFDK
MFEAFKVRSIEFGPVYK
ESIGPIDTVVISDPAEYAKVIRADGKFPNRKEMEPMAF
YREQKGIGLGLVNSQGEEWHRQRSAVIKKML
MLNEVQDFSQPMNVVANDFITHLSSSRDASGE
IQQLDKQIFKWAMESIGTFLFEERIGCLNEK
PTELAQEFIDTLQKYFRLMQQLMYNLPMYKVFRTKKWQEFEK
LSDRIMEVGREFVDKKIE
KLQNADPSTSQEKGAFLTHLISQKS
LSTSDVTSNAVDLLSAAVETTSNASLWCLYNLATN
PEAQQTMFNEINTVLPNKEDVTPQALSKLPYVKAVLK
ETFRKYPITYATSRFLPENLEVGGYNIPAG
THVQANLYGMYSD
AKIFPEPEKFKPERWLRESKMDSQT
KSLSNLIWGHGARMCIGRRFAEQEMHIMLTK
IIQNFKLEYHHEPVEPTLNT
VMTPDRPVQIKFVPRS
CYP10B1 Capitella capitata (Gallery worm, polychaete worm, annelid)
JGI Protein ID:143013
46% to CYP10A1
MKGGQVVRKEKKLKPFKDIPGPKG
LPVIGTLLEFTKKDGLKFNK
MFEVMTSRSKEFGPVYK
ERIGMIESVIVSDPHEYAKVIQVDGKHPHRIELFPMVH
YRQKKKMALGTVNAQGEEWYRGRMVLSKVML
KPKEVQDYVIAQSDVGNDFIRHMKSIVSSDGQ
LISFEREIFKWALESICVVLFEERIGCFPNP
PTEKAQEFITNLIGFFKYMQPLMYNFPMYKIYPTKTWRQYEK
HGDIVVGIG
LDMVRKVKKESKSEFLTYLLAQKS
LSPVEANSHAVDLMMGAVETTANSMMWMLYCLSNF
PDAQRKLQEEIDKIVPQDERITPEVLSKMKYTKACLK
ETFRLFPITFATSRMIHEEIELGGYHIPKG
THCQANLWGMGRD
PDYFSDPLTFKPERWLRDIQTLEHH
NPHAVLPFGHGARMCIGLRFAEQEIYIAVAK
LMKHFTVKHEGELLPVLNT
VMTPDRPVNFRFLLRTKPALSGLGDTKKPIAVELK
CYP10B2 Capitella capitata (Gallery worm, polychaete worm, annelid)
JGI Protein ID:134490
87% to CYP10B1, 47% to CYP10A1
KLKPFKDTPGPK
GLPVIGTLLEFTKKDGLKFNK
MFEVMTSRSKEFGSVYK
ERIGFLESVIVSDPDEYTKVIKVDGKYPHRIEMLPMVH
YRQKKKMALGTVNAQGEEWYRARMVLSKVML
KPKEVQEYVIAQSDVGNDFIKHMKSIASNDGQLLN
FEREIFKWALESICVVLFEERIGCFPNP
PTERAQEFIANLIGFFKYMQPLMYNLPVYKIYPTKTWRKYEK
HGDTVFGIGLDMV
TKVFLPGEKESKSEFLTYLLAQKS
LSPVEANSHAVDLMIGAVETTANSMMWMLYCLSNF
PEVQRKLQDEIDATLPQDEHITPEILSRLKYTRACLK
ETFRLYPVTFATSRMIHKDIELGGYHIPKG
THCQANLWGMGRD
PDYFSDPLTFKPERWQRDTQTLEYH
NPHAVLPFGHGARMCIGRRFAEQEIYIAVAK
LMKHFTVEHVGHLHPILIT
VMIPDRPVNFRFRPRTKPER
CYP10C1 Helobdella robusta (leech, annelid worm)
JGI Protein ID:67702
48% to CYP10B2, 41% to CYP10A1
MRKYIIQIISRNKIHNFNNYDRIGNNILKV
NCVSTSAAAFDNTDLLVSNANVLTENKLAELKSFESLPGPK
GLPLFGTLLSYVRPGGLSFRK
MFKVMDMRAKQYGSVYK
ETIGSNLTSVIVSDMEEYLKVIRADGKMPNRIELEPFKY
FRERNNFDIGIVNSQGLQWLEKRQILGPKIM
ALSEILDHVGPINDVTMDFLKRMDEKLLDRDGLFK
NLERNLFTWALEAVGTYLFEARIGCLNEP
IPPFADMFITHLSGMFREMQGLMYGPPIYKYISTRNWKRFED
HCYKIM
EAGMTLIEETSSSSALLTFLLSQKS
MSASSVNLTSIDVMIGAIETTSNSFTWMLYCLARN
PEVQEKLFEEVLQYLPNKDSPVTREVIRKMDYVRACLK
ETLRFYPITFATSRKLASEIELKGFRIPKG
THVQANLWTIGRD
ERLFPHPEKFNPDRWLRKSHEVSHLA
TALSHLPFGHGPRMCIGQRLAEMEIYTMVSK
LIRNFKVECAEKGEVQPILMT
VLTPDRPMMLKFKRR
11A Subfamily
CYP11A1 human
PIR A48733 (239 amino acids)
Matteson, K.J., Chung, B.C., Urdea, M.S. and Miller, W.L.
Study of cholesterol side-chain cleavage (20,22 desmolase)
deficiency causing congenital lipoid adrenal hyperplasia
using bovine-sequence P450scc oligodeoxyribonucleotide
probes.
Endocrinology 118, 1296-1305 (1986)
CYP11A1 human
GenEMBL M14565
Chung BC, Matteson KJ, Voutilainen R, Mohandas TK, Miller WL. (1986)
Human cholesterol side-chain cleavage enzyme, P450scc: cDNA cloning,
assignment of the gene to chromosome 15, and expression in the
placenta. Proc Natl Acad Sci U S A. 83, 8962-8966.
First complete human CYP11A1 cDNA
CYP11A1 rabbit
GenEMBL S59219 (1336bp) PIR A49189 (445 amino acids)
Yang,X., Iwamoto,K., Wang,M., Artwhol,J., Mason,J.I. and
Pang,S.
Inherited congenital adrenal hyperplasia in the rabbit is caused by
a deletion in the gene encoding cytochrome P450 cholesterol side
chain cleavage enzyme.
Endocrinol. 132, 1977-1982 (1993)
CYP11A1 bovine
PIR A42033 (18 amino acids)
Pikuleva, I.A., Lapko, A.G., Chashchin, V.L.
Functional reconstitution of cytochrome P-450-scc with hemin
activated with Woodward's reagent K. Formation of a
hemeprotein cross-link.
J. Biol. Chem. 267, 1438-1442 (1992)
CYP11A1 Bos taurus (cow)
See cattle page for details
MLARGLPLRSALVKACPPI
LSTVGEGWGHHRVGTGEGAGISTKTPRPYSEIPSPGDNGWLNLYHFWREKGSQRIHFRHI
ENFQKYGPIYREKLGNLESVYIIHPEDVAHLFKFEGSYPERYDIPPWLAYHRYYQKPIGV
LFKKSGTWKKDRVVLNTEVMAPEAIKNFIPLLNPVSQDFVSLLHKRIKQQGSGKFVGDIK
EDLFHFAFESITNVMFGERLGMLEETVNPEAQKFIDAVYKMFHTSVPLLNVPPELYRLFR
TKTWRDHVAAWDTIFNKAEKYTEIFYQDLRRKTEFRNYPGILYCLLKSEKMLLEDVKANI
TEMLAGGVNTTSMTLQWHLYEMARSLNVQEMLREEVLNARRQAEGDISKMLQMVPLLKAS
IKETLRLHPISVTLQRYPESDLVLQDYLIPAKTLVQVAIYAMGRDPAFFSSPDKFDPTRW
LSKDKDLIHFRNLGFGWGVRQCVGRRIAELEMTLFLIHILENFKVEMQHIGDVDTIFNLI
LTPDKPIFLVFRPFNQDPPQA*
CYP11A1 Sus scrofa (pig)
GenEMBL X13768 (see also NM_214427)
Mulheron GW, Stone RT, Miller WL, Wise T. (1989)
Nucleotide sequence of cytochrome P-450 cholesterol side-chain
cleavage cDNA isolated from porcine testis.
Nucleic Acids Res. 17, 1773
CYP11A1 Sus scrofa (pig)
GenEMBL L34259 (2376bp), NM_214427
Urban,R.J., Shupnik,M.A. and Bodenburg,Y.H.
Insulin-like growth factor-I increases expression of the porcine
P-450 cholesterol side chain cleavage gene through a GC-rich domain.
J. Biol. Chem. 269, 25761-25769 (1994)
MLARGLALRSVLVKGCQPFLSAPRECPGHPRVGTGEGACISTKT
PRPFSEIPSPGDNGWINLYRFWKEKGTQKIHYHHVQNFQKYGPIYREKLGNLESVYII
DPEDVALLFKFEGPNPERYNIPPWVAYHQHYQKPVGVLLKKSGAWKKDRLVLNTEVMA
PEAIKNFIPLLDTVSQDFVGVLHRRIKQQGSGKFSGDIREDLFRFAFESITNVIFGER
LGMLEEIVDPEAQKFIDAVYQMFHTSVPMLNLPPDLFRLFRTKTWRDHVAAWDTIFNK
AEKYTQNFYWDLRRKREFNNYPGILYRLLGNDKLLSEDVKANVTEMLAGGVDTTSMTL
QWHLYEMARSLNVQEMLREEVLNARRQAQGDTSKMLQLVPLLKASIKETLRLHPISVT
LQRYLVNDLVLRDYMIPAKTLVQVAVYAMGRDPAFFSNPGQFDPTRWLGKERDLIHFR
NLGFGWGVRQCVGRRIAELEMTLFLIHILENFKVELQHFSDVDTIFNLILMPDKPIFL
VFRPFNQDPLQA
CYP11A1 goat
GenEMBL D50058 (1825bp)
Okuyama,E., Okazaki,T., Furukawa,A., Wu,R.-F. and Ichikawa,Y.
Molecular cloning and nucleotide sequences of cDNA clones of sheep
and goat
adrenolcortical cytochrome P450scc.
unpublished (1995)
CYP11A1 sheep
GenEMBL D50057 (1825bp)
Okuyama,E., Okazaki,T., Furukawa,A., Wu,R.-F. and Ichikawa,Y.
Molecular cloning and nucleotide sequences of cDNA clones of sheep
and goat
adrenolcortical cytochrome P450scc.
unpublished (1995)
CYP11A1 bovine
PIR S29644 (21 amino acids)
Tsujita, M. and Ichikawa, Y.
Substrate-binding region of cytochrome P-450(scc) (P-450
XIA1). Identification and primary structure of the
cholesterol binding region in cytochrome P-450(scc).
Biochim. Biophys. Acta 1161, 124-130 (1993)
Cyp11a1 mouse
GenEMBL J05511, NM_019779, AF195119
Rice,D.A., Kirkman,M.S., Aitkin,L.D., Mouw,A.R., Schimmer,B.P. and
Parker,K.L.
Analysis of the promoter region of the gene encoding mouse
cholesterol side-chain cleavage enzyme
J. Biol. Chem. 265, 11713-11720 (1990)
MLAKGLSLRSVLVKGCQPFLSPTWQGPVLSTGKGAGTSTSSPRS
FNEIPSPGDNGWLNLYHFWRESGTQKIHYHQMQSFQKYGPIYREKLGTLESVYIVDPK
DASILFSCEGPNPERFLVPPWVAYHQYYQRPIGVLFKSSDAWKKDRIVLNQEVMAPGA
IKNFVPLLEGVAQDFIKVLHRRIKQQNSGNFSGVISDDLFRFSFESISSVIFGERMGM
LEEIVDPEAQRFINAVYQMFHTSVPMLNLPPDFFRLLRTKTWKDHAAAWDVIFNKADE
YTQNFYWDLRQKRDFSQYPGVLYSLLGGNKLPFKNIQANITEMLAGGVDTTSMTLQWN
LYEMAHNLKVQEMLRAEVLAARRQAQGDMAKMVQLVPLLKASIKETLRLHPISVTLQR
YTVNDLVLRNYKIPAKTLVQVASFAMGRDPGFFPNPNKFDPTRWLEKSQNTTHFRYLG
FGWGVRQCLGRRIAELEMTILLINLLENFRIEVQNLRDVGTKFSLILMPENPILFNFQ
PLKQDLGPAVTRKDNTVN
CYP11A1 Xenopus tropicalis
See Xenopus pages for seq
CYP11A1 Danio rerio (zebrafish)
See xebrafish pages for seq
CYP11A1 Oncorhynchus mykiss (rainbow trout)
GenEMBL S57305 (1789bp) Swiss Q07217 (514 amino acids)
PIR S32197 (514 amino acids)
Takahashi,M., Tanaka,M., Sakai,N., Adachi,S., Miller,W.L.
and Nagahama,Y.
Rainbow trout ovarian cholesterol side-chain cleavage
cytochrome P450 (P450scc). cDNA cloning and mRNA expression
during oogenesis.
FEBS Lett. 319, 45-48 (1993)
CYP11A1 Fugu rubripes (pufferfish)
No accession number
Scaffold_1630
37515 MARWSVWRSPVVLPLSRMEVPMTGARHSSTMPVARQTYSDSSSFV 37381
37380 RSFNDIPGLWKNGVANLYNFWKLDGFRNLHHIMVQNFNTFGPIYR 37246 (2)
37065 EKIGYYESVNIINPEDAAILFKAEGHYPKRLKVEAWTSYRDYRNRKYGVLLK 36904 (2)
36822 NGEEWRCNRVLLNKEVISPKVLENFVPLLDEVGNDFVVRVHKKIARSGQNKWTTDLSQELFKYALES 36628 (1)
36506 VSSVLYGERLGLFLDYIDPEAQHFIDCISLMFKTTSPML
YIPPALLRKVGAKVWRDHVEAWDGIFNQ 36300 (1 expected) bad boundary
36208 ADRCIQNIYRRLRQETGPSKKYPGVLASLLLRDKLSIEDIKASITELMAGGVDT 36047 (0)
35975 TSITLLWTLYELARHPNLQEELRAEVAAARTESQGDMLEMLKRIPLVKGALKETLR 35808 (2)
35731 LHPVAVSLQRYIAEDIIIQNYHIPAG 35654 (0)
35568 TLVQLGLYAMGRDPKVFFRPEQYQPSRWLRSETHYFKSLGFGFGPRQCLGRRIAEAEMQLFLIH 35377 (0)
35297 MLENFRVEKQRHMEVQSTFELILLPDKPIILTLKPLSS* 35181
CYP11A1 Dasyatis americana (southern stingray)
GenEMBL U63299(4619bp)
Nunez,B.S. and Trant,J.M.
Isolation of the cDNA encoding the interrenal form of cholesterol
side chain cleavage cytochrome P450 of the southern stingray
(Dasyatis americana).
unpublished (1996)
CYP11A2 Danio rerio (zebrafish)
See xebrafish pages for seq
11B Subfamily
CYP11B1 human
GenEMBL J05140 M32863 (1482bp) M32878 (2633bp) M32879 (1155bp)
Mornet,E., Dupont,J., Vitek,A. and White,P.C.
Characterization of two genes encoding human steroid 11-beta-
hydroxylase (P-450-11-beta).
J. Biol. Chem. 264, 20961-20967 (1989)
CYP11B1 human
GenEMBL D16155 (156bp)
Naiki,Y., Shizuta,Y., Kawamoto,T., Yasushiro,M., Miyahara,K.,
Toda,K., Tadao,O. and Imura,H.
A nonsense mutation (TGG(116Arg)-TAG(stop)) in CYP11B1
causes steroid 11beta-hydroxylase deficiency.
J. Clin. Endocrinol. Metab. 77, 1677-1682 (1993)
CYP11B1 human
GenEMBL D10169 D90428 (2085bp)
Kawamoto,T., Mitsuuchi,Y., Toda,K., Yokoyama,Y., Miyahara,K.,
Miura,S., Ohnishi,T., Ichikawa,Y., Nakao,K., Imura,H., Ulick,S.
and Shizuta,Y.
Role of steroid 11beta-hydroxylase and steroid 18-hydroxylase in the
biosynthesis of glucocorticoids and mineralocorticoids in humans.
Proc. Natl. Acad. Sci. USA 89, 1458-1462 (1992)
CYP11B1 human
PIR S29068 (30 amino acids)
Kawamoto, T., Mitsuuchi, Y., Toda, K., Miyahara, K.,
Yokoyama, Y., Nakao, K., Hosoda, K., Yamamoto, Y., Imura,
H. and Shizuta, Y.
Cloning of cDNA and genomic DNA for human cytochrome P-450
(11-beta).
FEBS Lett. 269, 345-349 (1990)
CYP11B1 Papio hamadryas ursinus (chacma baboon)
GenEMBL U52085(228bp)
Hampf,M., Swart,A. and Swart,P.
Expression of Papio ursinus steroid 11beta hydroxylase
unpublished (1996)
CYP11B1 rat
GenEMBL D10107 (1528bp) S58847
PIR B46040 (499 amino acids)
Matsukawa,N., Nonaka,Y., Higaki,J., Nagano,M., Mikami,H.,
Ogihara,T. and Okamoto,M.
Dahl's salt-resistant normotensive rat has mutations in
cytochrome P450 (11 beta), but the salt-sensitive hypertensive
rat does not.
J. Biol. Chem. 268, 9117-9121 (1993)
Note: only 1 amino acid difference with 11B1 at position 84
E (normal) changed to G (seen in 11B2 and 11B3)
D10107 has five differences with D11354
CYP11B1 rat
GenEMBL S58858 (330bp) PIR JX0251(499 amino acids)
Nomura,M., Morohashi, K.-i., Kirita, S., Nonaka, Y.,
Okamoto,M., Nawata,H. and Omura,T.
Three forms of rat CYP11B genes: 11 beta-hydroxylase gene,
aldosterone synthase gene, and a novel gene.
J. Biochem. 113, 144-152 (1993)
Note: Fig. 3 has 6 errors. 1,2) aldo-46 amino acids 2 and 3 are
incorrectly translated gctctc = AL not HS. 3) 11B3 codon at 559-561
incorrectly translated gac = D not N. 4,5,6) 11beta-62, 11B1
and 11B3 codon at 964-966 tcc = Ser not Pro.
CYP11B1 rat
GenEMBL S58849, D14086 to D14091 PIR A46039 (499 amino acids)
Mukai,K., Imai,M., Shimada,H. and Ishimura,Y.
Isolation and characterization of rat CYP11B genes involved
in late steps of mineralo- and glucocorticoid syntheses.
J. Biol. Chem. 268, 9130-9137 (1993)
CYP11B1 rat
GenEMBL S63899 (596bp)
Mukai,K., Imai,M., Shimada,H., Okada,Y., Ogishima,T. and
Ishimura,Y.
Structural differences in 5'-flanking regions of rat cytochrome
P-450aldo and P-450(11) beta genes
Biochem. Biophys. Res. Commun. 180, 1187-1193 (1991)
CYP11B1 rat
GenEMBL D11354 (1528bp) PIR A46040 (499 amino acids)
Matsukawa,N., Nonaka,Y., Higaki,J., Nagano,M., Mikami,H.,
Ogihara,T. and Okamoto,M.
Dahl's salt-resistant normotensive rat has mutations in
cytochrome P450 (11 beta), but the salt-sensitive hypertensive
rat does not.
J. Biol. Chem. 268, 9117-9121 (1993)
Note: This sequence is the wild type DS rat sequence. The mutant
DR rat has five amino acids differences accession number D10107
CYP11B1 Cavia porcellus (guinea pig)
GenEMBL Z69785(2028bp)
Bulow,H.E., Mobius,K., Bahr,V. and Bernhardt,R.
Molecular cloning and functional expression of the cytochrome P450
11B-hydroxylase of the guinea pig.
Biochem. Biophys. Res. Commun. 221, 304-312 (1996)
CYP11B1 bovine
PIR JX0151 (503 amino acids)
Kirita, S., Hashimoto, T., Kitajima, M., Honda, S.,
Morohashi, K. and Omura, T.
Structural analysis of multiple bovine P-450(11beta) genes
and their promoter activities.
J. Biochem. 108, 1030-1041 (1990)
CYP11B1 Bos taurus (cow)
See cattle page for details
MALWAKARVRMAGPWLSLHEARLLGTRGAAAPKAVLPFEAMPRC
PGNKWMRMLQIWKEQSSENMHLDMHQTFQELGPIFRYDVGGRHMVFVMLPEDVERLQQ
ADSHHPQRMILEPWLAYRQARGHKCGVFLLNGPQWRLDRLRLNPDVLSLPALQKYTPL
VDGVARDFSQTLKARVLQNARGSLTLHIAPSVFRYTIEASTLVLYGERLGLLTQQPNP
DSLNFIHALEAMLKSTVQLMFVPRRLSRWMSTNMWREHFEAWDYIFQYANRAIQRIYQ
ELALGHPWHYSGIVAELLMRADMTLDTIKANTIDLTAGSVDTTAFPLLMTLFELARNP
EVQQAVRQESLVAEARISENPQRAITELPLLRAALKETLRLYPVGITLEREVSSDLVL
QNYHIPAGTLVKVLLYSLGRNPAVFARPESYHPQRWLDRQGSGSRFPHLAFGFGVRQC
LGRRVAEVEMLLLLHHVLKNFLVETLEQEDIKMVYRFILMPSTLPLFTFRAIQ
CYP11B1 pig
GenEMBL D38590(1671bp)
Sun,T., Zhao,Y., Nonaka,Y. and Okamoto,M.
Cloning and expression of cytochrome P450(11 beta) of porcine
adrenal cortex.
J. Steroid Biochem. Mol. Biol. 52, 227-232 (1995)
MAIWAKAEAWLAGPWLALNRARTLGTRAVLAPKGVLPFEAIPQF
PGKKWMRVLQLWREQGFENNHLEMHQTFQELGPIFRFDVGGRNMVLVMLPEDVERCQK
VEGLHPQRDVPGPWLAYRHLRGHKCGVFLLNGPTWRLDRLQLNPGVLSLQAMQKFTPL
VDGVARDFSQALRARVMQNARGSLTLDIKPSIFRYTIEASNLVLFGERLGLLAHQPNP
ESLDFIHALEVMFKSTVQLMFMPRSLSRWTSTGTWKEHFEAWDCIFQYANKAIQRLYQ
ELTLGHPWHYSGVVAELLTHANMTVDAIKANSIDLTAGSVDTTAYPLLMTLFELARNP
EVQQALRQESLAAAARISENPQKAITELPLLRAALKETLRLYPVGIFLDRCVTSDLVL
QNYHIPAGTLVKVLLYSLGRNPAVFARPERYHPQRWLDNQGSGTRFPHLAFGFGMRQC
LGRRLAQVEMLLLLHHVLKNFLVETLVQEDIKMIYRFIMTPSTLPLLTFRAIS
CYP11B1 sheep
GenEMBL L34337 (1639bp)
Boon,W.C., Roche,P.J., Hammond,V.E., Jeyaseelan,K.,
Crawford,R.J. and Coghlan,J.P.
Cloning and expression analysis of a cytochrome P450-11-beta
cDNA in sheep.
unpublished (1994)
CYP11B1 Ovis aries (sheep)
GenEMBL L28716 (2300bp)
Anwar,A., Jeyaseelan,K. and Coghlan,J.P.
Molecular cloning and characterization of the ovine CYP11B1
promoter.
Biochem. Mol. Biol. Int. 33, 1169-1178 (1994)
CYP11B1 Ovis ammon (sheep)
GenEMBL L47569(9027bp)
Anwar,A., Jeyaseelan,K. and Coghlan,J.P.
Characterization of an ovine 11-beta hydroxylase (cyp11b) gene.
unpublished (1995)
Cyp11b1 mouse
PIR A41552 (500 amino acids)
Domalik, L.J., Chaplin, D.D., Kirkman, M.S., Wu, R.C., Liu,
W., Howard, T.A., Seldin, M.F., Parker, K.L.
Different isozymes of mouse 11beta-hydroxylase produce
mineralocorticoids and glucocorticoids.
Mol. Endocrinol. 5, 1853-1861 (1991)
Cyp11b1 mouse
PIR A32210 (42 amino acids)
Mouw, A.R., Rice, D.A., Meade, J.C., Chua, S.C., White, P.C.,
Schimmer, B.P. and Parker, K.L.
Structural and functional analysis of the promoter region of
the gene encoding mouse steroid 11beta-hydroxylase.
J. Biol. Chem. 264, 1305-1309 (1989)
Cyp11b1 mouse
GenEMBL J04451
80% to mouse 11B2 64% to human 11B1 and 11B2 80% to 11B1 rat
78% to 11B2 rat. sequence below is from Ensembl mouse version 3
15.75000001-76000000 mouse chr 15 add 75 million to get global location
306415 MALRVTTDVWLARPWQCLHRTRALGTTATLAPKTLQPFEAIPQYSRNKWLKMIQILREQG 306356
306355 QENLHLEMHQVFRELGPIFR 306176 (?)
305773 HSVGKTQIVFVTLPEDVEKLYQVESTHPCRMPLESWIVHRELRGLGRGVFLL 305618 (?)
304234 NGPEWYFNRLQLNPNVLSPKAVQKFVPLVDGIARDFVDNLKKKMLESVHGSFSMDFQSSV 304175
304174 FNYTIE 304157 (?)
303918 ASHFVLFGERLGLIGRDLSPDSLKFLHTLHSMFKTTTQLLYLPRSLTRWTSTRVWKENLE 303859
303858 SWDFISEYG 303826 (?)
303446 TKCIKNVYRELAEGRPQSWSVTAELVAERTLSMDAIQANSMELIAGSTDT 303413 (0?)
301935 TSTPLVMTFFELARNPDVQQALRQESLAAEASIAANPQKAMSDLPLLRAALKETLR 301768
301535 LYPVGTFLERILSSDLVLQNYHVPAG 301458
301370 TVLNVNLYSMGRNPAVFPRPERYMPQRWLERKRSFKHLAFGFGVRQCLGRRLAEAEM 301314
301313 MLLLHH 301182 (?0)
300923 VLKSFHVETQEK 300888
300887 EDVRMAYRFVLMPSSSPLLTFRPVN 300705
CYP11B1 Xenopus tropicalis
See Xenopus pages for seq
CYP11B1 Danio rerio (zebrafish)
See xebrafish pages for seq
CYP11B2 human
GenEMBL J05140 M32864 (1809bp) M32880 (3088bp) M32881 (1101bp)
Mornet,E., Dupont,J., Vitek,A. and White,P.C.
Characterization of two genes encoding human steroid 11-beta-
hydroxylase (P-450-11-beta).
J. Biol. Chem. 264, 20961-20967 (1989)
CYP11B2 human
GenEMBL D90429 D10170 (2114bp)
Kawamoto,T., Mitsuuchi,Y., Toda,K., Yokoyama,Y., Miyahara,K.,
Miura,S., Ohnishi,T., Ichikawa,Y., Nakao,K., Imura,H., Ulick,S.
and Shizuta,Y.
Role of steroid 11beta-hydroxylase and steroid 18-hydroxylase in the
biosynthesis of glucocorticoids and mineralocorticoids in humans.
Proc. Natl. Acad. Sci. USA 89, 1458-1462 (1992)
CYP11B2 human
GenEMBL D13752(6910bp)
Kawamoto,T., Mitsuuchi,Y., Ohnishi,T., Ichikawa,Y., Yokoyama,Y.,
Sumimoto,H., Toda,K., Miyahara,K., Kuribayashi,I., Nakao,K.,
Hosoda,K., Yamamoto,Y., Imura,H. and Shizuta,Y.
Cloning and expression of a cDNA for human cytochrome P-450aldo as
related to primary aldosteronism.
Biochem. Biophys. Res. Commun. 173 (1), 309-316 (1990)
CYP11B2 human
GenEMBL S77398 S77401 S77403 S77406 S77409 (genomic sequences)
Shizuta,Y., Kawamoto,T., Mitsuuchi,Y., Miyahara,K., Rosler,A.,
Ulick,S. and Imura,H.
Inborn errors of aldosterone biosynthesis in humans.
Steroids 60 (1), 15-21 (1995)
CYP11B2 Bos taurus (cow)
See cattle page for details
MALWAKARVRMAGPWLSLHEARLLGTRGAAAPKAVLPFEAMPRCPGNKWMRMLQIWKE
QSSENMHLDMHQTFQELGPIFRYDVGGRHMVFVMLPEDVERLQQADSHHPQRMILEPWLA
YRQARGHKCGVFLLNGPQWRLDRLRLNPDVLSLPALQKYTPLVDGVARDFSQTLKARVLQ
NARGSLTLDIAPSVFRYTIEASTLVLYGERLGLLTQQPNPDSLNFIHALEAMLKSTVQLM
FVPRRLSRWMSTNMWREHFEAWDYIFQYANRAIQRIYQELALGHPWHYSGIVAELLMRAD
MTLDTIKANTIDLTAGSVDTTAFPLLMTLFELARNPEVQQAVRQESLVAEARISENPQRA
ITELPLLRAALKETLRLYPVGITLEREVSSDLVLQNYHIPAGTLVKVLLYSLGRNPAVFA
RPESYHPQRWLDRQGSGSRFPHLAFGFGVRQCLGRRVAEVEMLLLLHHVLKNFLVETLEQ
EDIKMVYRFILMPSTLPLFTFRAIQ*
CYP11B2 Sus scrofa (pig)
ESTs CV872108.1, BI341717.1
93% to 11B1 pig
MAIWAKAEAWLAGPWLALHRARTLGTRAALAPKAVLPFEAIPQCPGNKWMRVLQLWREQG
FENIHLDMHQTFQELGPIFRFDVGGRHMVLVMLPEDVERLQKVEGLHPQRMFLEPWLAYR
QLRGHKCGVFLLNGPTWRLDRLQLNPGVLSLQAMQKFTPLVDGVARDFSQALRARVMQNA
RGSLTLDIKPSIFRYTIEASNLVLFGERLGLLAHQPNPES
CYP11B2 rat
GenEMBL S58859 (353bp) PIR JX0252 (500 amino acids)
Nomura,M., Morohashi, K.-i., Kirita, S., Nonaka, Y.,
Okamoto,M., Nawata,H. and Omura,T.
Three forms of rat CYP11B genes: 11 beta-hydroxylase gene,
aldosterone synthase gene, and a novel gene.
J. Biochem. 113, 144-152 (1993)
CYP11B2 rat
GenEMBL S58850 D14092 to D14097
PIR B46039 (500 amino acids)
Mukai,K., Imai,M., Shimada,H. and Ishimura,Y.
Isolation and characterization of rat CYP11B genes involved
in late steps of mineralo- and glucocorticoid syntheses.
J. Biol. Chem. 268, 9130-9137 (1993)
CYP11B2 rat
GenEMBL S63898 (594bp)
Mukai,K., Imai,M., Shimada,H., Okada,Y., Ogishima,T. and Ishimura,Y.
Structural differences in 5'-flanking regions of rat cytochrome
P-450aldo and P-450(11) beta genes.
Biochem. Biophys. Res. Commun. 180, 1187-1193 (1991)
CYP11B2 rat
GenEMBL S64136 (3001bp) PIR JN0615 (506 amino acids)
GenEMBL U14908 (3000bp)
Zhou,M. and Gomez-Sanchez,C.E.
Cloning and expression of a rat cytochrome P-450 11
beta-hydroxylase/aldosterone synthase (CYP11B2) cDNA variant.
Biochem. Biophys. Res. Commun. 194, 112-117 (1993)
Erratum: Biochem. Biophys. Res. Commun. 196, 1018 (1993)
CYP11B2 rat
PIR A34281 (20 amino acids) Swiss P30099 (510 amino acids)
Ogishima, T., Mitani, F., Ishimura, Y.
Isolation of aldosterone synthase cytochrome P-450 from zona
glomerulosa mitochondria of rat adrenal cortex.
J. Biol. Chem. 264, 10935-10938 (1989)
Note: sequence is from N-terminal after signal sequence
CYP11B2 rat
Swiss P30099 (510 amino acids) GenEMBL D00567 (2824bp)
Swiss P30100 (500 amino acids) GenEMBL D00568 (2705bp)
Matsukawa,N., Nonaka,Y., Ying,Z., Higaki,J., Ogihara,T. and
Okamoto,M.
Molecular cloning and expression of cDNAs encoding rat aldosterone
synthase: variants of cytochrome P-450 11beta
Biochem. Biophys. Res. Commun. 169, 245-252 (1990)
CYP11B2 hamster
GenEMBL S73810 (1503bp)
LeHoux,J.G., Mason,J.I., Bernard,H., Ducharme,L., LeHoux,J.,
Veronneau,S. and Lefebvre,A.
The presence of two cytochrome P450 aldosterone synthase mRNAs in
the hamster adrenal.
J. Steroid Biochem. Mol. Biol. 49, 131-137 (1994)
CYP11B2 cavia porcellus (domestic guinea pig)
GenEMBL AF018569
Buelow,H.E. and Bernhardt,R.
Molecular Cloning and Functional Expression of the Guinea Pig
Aldosterone Synthase.
Unpublished
CYP11B2 Rana catesbeiana (bullfrog)
GenEMBL D10984 (1919bp)
Nonaka,Y., Takemori,H., Halder,S.K., Sun,T., Ohta,M., Hatano,O.,
Takakusa,A. and Okamoto,M.
Frog Cytochrome P-450 (11 beta, aldo), a single enzyme involved in
the final steps
of glucocorticoid and mineralocorticoid biosynthesis.
Eur. J. Biochem. 229, 249-256 (1995)
Cyp11b2 mouse
GenEMBL S85260 (2804bp)
Domalik,L.J., Chaplin,D.D., Kirkman,M.S., Wu,R.C., Liu,W.W.,
Howard,T.A., Seldin,M.F. and Parker,K.L.
Different isozymes of mouse 11 beta-hydroxylase produce
mineralocorticoids and glucocorticoids.
Mol. Endocrinol. 5, 1853-1861 (1991)
Cyp11b2 mouse
genEMBL S85260
68% to human 11B2 67% to human 11b1 94% to 11B2 rat 81% to 11B1 rat
sequence below is from Ensembl mouse version 3
15.75000001-76000000 mouse chr 15 add 75million to get global location
321034 MALRVTADVWLARPWQCLHRTRALGTTATLAPKTLQPFEAIPQYSRNKWLKMIQILREQG 320975
320974 QENLHLEMHQVFRELGPIFR 320795 (?)
320391 HSVGKTQIVSVMLPEDAEKLHQVESMLPRRMHLEPWVAHRELRGLRRGVFLL 320238 (?)
318803 NGPEWRLNRLRLNRNVLSPKAVQKFVPMVDMVARDFLETLKEKVLQNARGSLTM 318762
318761 DVQQSLFNYTIE 318606 (?)
318477 ASNFALFGERLGLLGHDLSPGSLKFIHALHSMFKSTS 318487
318486 QLLFLPKSLTRWTSTRVWKEHFDAWDVISEYG (?) 318391
318000 NRCIWKVHQELRLGSSQTYSGIVAELISQGSLPLDAIKA 318004
318003 NSMELTAGSVDT 317848 (?)
316981 TAIPLVMTLFELARNPDVQKALRQESLAAEASIAANPQKAMSDLPLLRAALKETLR 316814 (?)
316581 LYPVGGFLERILSSDLVLQNYHVPAG 316504 (0?)
316416 TLVLLYLYSMGRNPAVFPRPERYMPQRWLERKRSFQHLAFGFG 316396
316395 VRQCLGRRLAEVEMMLLLHH 316228 (0?)
315961 ILKTFQVETLRQEDVQMAYRFVLMPSSSPVLTFRPVS 315851
CYP11B3 rat
PIR JX0253 (498 amino acids)
Nomura,M., Morohashi, K.-i., Kirita, S., Nonaka, Y.,
Okamoto,M., Nawata,H. and Omura,T.
Three forms of rat CYP11B genes: 11 beta-hydroxylase gene,
aldosterone synthase gene, and a novel gene.
J. Biochem. 113, 144-152 (1993)
CYP11B3 rat
GenEMBL U14907 (1497bp)
Zhou,M., Gomez-Sanchez,E.P., Foecking,M. and Gomez-Sanchez,C.E.
Cloning of the cytochrome P-450 CYP11B3 complementary DNA in the
rat.
unpublished
CYP11B3 rat
GenEMBL S59144 D14098 to D14103
Mukai,K., Imai,M., Shimada,H. and Ishimura,Y.
Isolation and characterization of rat CYP11B genes involved
in late steps of mineralo- and glucocorticoid syntheses.
J. Biol. Chem. 268, 9130-9137 (1993)
Note: only one amino acid difference with Nomura's 11B3
CYP11B3 rat
GenEMBL U17082(1497bp)
Mellon,S.H., Bair,S.R. and Monis,H.
P450c11B3 mRNA, transcribed from a third P450c11 gene, is expressed
in a tissue-specific, developmentally, and hormonally regulated
fashion in the rodent adrenal and encodes a protein with both
11-hydroxylase and 18-hydroxylase activities.
J. Biol. Chem. 270, 1643-1649 (1995)
Note Genbank has a seq from rat named CYP11B4 D14108
This is part of the CYP11B8P pseudogene (see below)
CYP11B4 cattle
Kirita et al. 1990 J. Biochem (Tokyo) 108, 1030-1041.
Listed in 1996 nomenclature update
As having the same coding region sequence
But different flanking regions
CB11beta-20
CYP11B5P cattle
GenEMBL JU0316
Kirita et al. 1990 J. Biochem (Tokyo) 108, 1030-1041.
CB11beta-1 pseudogene
CYP11B6P cattle
GenEMBL JU0317
Kirita et al. 1990 J. Biochem (Tokyo) 108, 1030-1041.
CB11beta-3 pseudogene
CYP11B7P cattle
GenEMBL JU0318, D00458, D00459
Hashimoto et al. 1989 J. Biochem (Tokyo) 105, 676-679.
LambdaB11beta(15-1) and (15-2)
Kirita et al. 1990 J. Biochem (Tokyo) 108, 1030-1041.
CB11beta-21 pseudogene
CYP11B8P rat pseudogene
GenEMBL D14104 to D14108
Mukai,K., Imai,M., Shimada,H. and Ishimura,Y.
Isolation and characterization of rat CYP11B genes involved
in late steps of mineralo- and glucocorticoid syntheses.
J. Biol. Chem. 268, 9130-9137 (1993)
Note: authors call this sequence 11B4
12A Subfamily
CYP12A1 Musca domestica (housefly)
GenEMBL U86618
Rene Feyereisen
MIKYKQYSRAIVALRQRGAQQYSTNVTNASQPDVKATTTTTISP
EWQEAKPFEEMPSMNSWPIIKNMLPWGKYGKMEPTQFLMALRDDMGPIVRTAAFMGRP
PTVITHNPHDFEMVFRNEGIWPIRPGGDAQMYHRTVLREDFFQGVTGLVSVNGEKWGN
FRSTVNPVLMQPKNVRLYLNKMAQVNDEFMARIRQIRDPETLEVPASFQEEMNRWTLE
SVSVVALDKQLGLITTNRDNPDLKKLIGLLNDFFELGQKIEFGLPFWKYIKTPTFKLF
MKTLDGLLEIGNKYVNEAIDRLEAERQSGVPEKPENEKSVLEKLIKIDRKIATVMAID
MILAGVDTTSTTFTALLLCLAKNPEKQEKLREEIRQILPRKDSQFEPSSLNHIPYTRA
CIKEALRMYPLTLGNARILANDTVLSGYRVPKGTLVSMISTGLLQDDNHYTKAKEYLP
ERWMRPTKEETEDSATCPHALKASSPFIYLPFGFGPRSCVGRRIVEMELELGIARLVR
NFRIEFNYPTENAFKFKLINVPNIPLKFKFTDVEN
CYP12A2 Musca domestica (housefly)
GenEMBL U94698
Rene Feyereisen
CYP12A3 Musca domestica (housefly)
GenEMBL U94699
Rene Feyereisen
cyp12a4 Drosophila melanogaster
GenEMBL AC006091 85973-87865 also AC015190 AC008141
chromosome 3 clone BACR48G05 (D475)
MLKVRSALSLIQSQKATLSLATQKHTEYFKILLYIYINKLYYQRWQTNVATAEAREDSEW
LQAKPFEQIPRLNMWALSMKMSMPGGKYKNMELMEMFEAMRQDYGDIFFMPGIMGNPPFL
STHNPQDFEVVFRNEGVWPNRPGNYTLLYHREEYRKDFYQGVMGVIPTQGKPWGDFRTVV
NPVLMQPKNVRLYYKKMSQVNQEFVQRILELRDPDTLEAPDDFIDTINRWTLESVSVVAL
DKQLGLLKNSNKESEALKLFHYLDEFFIVSIDLEMKPSPWRYIKTPKLKRLMRALDGIQE
VTLAYVDEAIERLDKEAKEGVVRPENEQSVLEKLLKVDRKVATVMAMDMLMAGVDTTSST
FTALLLCLAKNPEKQARLREEVMKVLPNKNPEFTEASMKNVPYLRACIKESQRLHPLIVG
NARVLARDAVLSGYRVPAGTYVNIVPLNALTRDEYFPQASEFLPERWLRSPKDSESKCPA
NELKSTNPFVFLPFGFGPRMCVGKRIVEMELELGTARLIRNFNVEFNYPTENAFRSALIN
LPNIPLKFKFIDLPN
Cyp12a4 Drosophila pseudoobscura
See D. pseudoobscura page on this server for sequence
Obtained from UC Santa Cruz Browser
cyp12a5 Drosophila melanogaster
GenEMBL AC006091 83449-85296 also AC015190 AC008141
chromosome 3 clone BACR48G05 (D475)
76% identical to other AC006091 SEQ. 58% TO 12A1, 12A2
MLKGRIALNILQSQKPIVFSASQQ*RWQTNVPTAEIRNDPEWLQAKPFEE
IPKANILSLFAKSALPGGKYKNLEMMEMIDALRQDYGNIIFLPGMMGRDG
LVMTHNPKDFEVVFRNEGVWPFRPGSDILRYHRTVYRKDFFDGVQGIIPS
QGKSWGDFRSIVNPVLMQPKNVRLYFKKMSQVNQEFIKEIRDASTQEVPG
NFLETINRWTLESVSVVALDKQLGLLRESGKNSEATKLFKYLDEFFLHSA
DLEMKPSLWRYFKTPLLKKMLRTMDSVQEVTLKYVDEAIERLEKEAKEGV
VRPEHEQSVLEKLLKVDKKVATVMAMDMLMAGVDTTSSTFTALLLCLAKN
PEKQARLREEVMKVLPNKDSEFTEASMKNVPYLRACIKESQRVYPLVIGN
ARGLTRDSVISGYRVPAGTIVSMIPINSLYSEEYFPKPTEFLPERWLRNA
SDSAGKCPANDLKTKNPFVFLPFGFGPRMCVGKRIVEMELELGTARLIRN
FNVEFNHSTKNAFRSALINLPNIPLKFKFKFTDVPN*
Cyp12a5 Drosophila pseudoobscura
See D. pseudoobscura page on this server for sequence
Obtained from UC Santa Cruz Browser
CYP12A6 Drosophila wassermani
no accession number
Tina Yee and Phil Danielson
59% to 12A2 56% to 12A1
N-terminal does not match known P450 sequences may be in error
CYP12A7 Lucilia cuprina (Australian blowfly)
No accession number
Zhenzhong Chen
Submitted to nomenclature committee March 17, 2005
revised to full length June 27, 2005
Clone name Lc_CYP12A2
59% to 12A5
CYP12A8 Lucilia cuprina (Australian blowfly)
No accession number
Zhenzhong Chen
Submitted to nomenclature committee March 17, 2005
Clone name Lc_CYP12A1
60% to 12A5 67% to 12A7 (365 aa)
CYP12A9 Lucilia cuprina (Australian blowfly)
No accession number
Zhenzhong Chen
Submitted to nomenclature committee March 17, 2005
Revised seq Dec 12, 2007
Old Clone name Lc_CYP12A3
New clone name Luce0104J9 (408 aa)
74% identical to Musca Domestica CYP12A2
only 1 aa diff to earlier shorter version
CYP12A10 Lucilia cuprina (Australian blowfly)
No accession number
Zhenzhong Chen
Submitted to nomenclature committee March 17, 2005
Clone name Lc_CYP12A4
57% to 12A4 65% to 129A9 (126 aa)
CYP12A11 Lucilia cuprina (Australian blowfly)
No accession number
Zhenzhong Chen
Submitted to nomenclature committee Dec. 18, 2007
clone name Luce0102F03
62% identical to CYP12A2 and 57% identical to Dm CYP12a5,
63% to 12A7, 72% to 12A8 partial seq, 64% to 12A9 partial,
67% to 12A10 partial seq.
12B Subfamily
CYP12B1 Drosophila acanthoptera
no accession number
Phil Danielson
Ac40
submitted to nomenclature committee
Cyp12b2 Drosophila melanogaster
GenEMBL AC018326 7227-9141 also AC004345 AC004657
77% identical to 12b1
Cyp12b2 Drosophila pseudoobscura
See D. pseudoobscura page on this server for sequence
Obtained from UC Santa Cruz Browser
Cyp12c1 Drosophila melanogaster
GenEMBL AC009385 comp(57935-59646) also AC012807
Cyp12c1 Drosophila pseudoobscura
See D. pseudoobscura page on this server for sequence
Obtained from UC Santa Cruz Browser
Cyp12d1 Drosophila melanogaster
GenEMBL AC008187 comp(84371-86114)
Cyp12d1 Drosophila pseudoobscura
See D. pseudoobscura page on this server for sequence
Obtained from UC Santa Cruz Browser
CYP12D2 Lucilia cuprina (Australian blowfly)
No accession number
Zhenzhong Chen
Submitted to nomenclature committee Dec. 18, 2007
clone name Lce0054K20
53% identity to Dm CYP12d1
probable ortholog of Cyp12d1
Cyp12e1 Drosophila melanogaster
GenEMBL AC018294 2070-3932
Cyp12e1 Drosophila pseudoobscura
See D. pseudoobscura page on this server for sequence
Obtained from UC Santa Cruz Browser
CYP12F1 Anopheles gambiae (malaria vector)
No accession number
Submitted by Rene Feyereisen July 5, 2002
Low 40% range with other CYP12s 60% to 12F2
CYP12F1 Anopheles gambiae (malaria mosquito)
GenEMBL
Submitted by Christelle Abgrall, Hilary Ranson and Rene Feyereisen
See the paper: Evolution of supergene families associated with
insecticide resistance.
Ranson, H., Claudianos, C., Ortelli, F., Abgrall, C., Hemingway, J.
Sharakhova, M.V., Unger, M.F., Collins, F.H. and Feyereisen, R.
Science 298, 179-181 (2002)
Anopheles map name CYPl3r4
CYP12F2 Anopheles gambiae (malaria vector)
No accession number
Submitted by Rene Feyereisen July 5, 2002
Low 40% range with other CYP12s 60% to 12F1
CYP12F2 Anopheles gambiae (malaria mosquito)
GenEMBL
Submitted by Christelle Abgrall, Hilary Ranson and Rene Feyereisen
See the paper: Evolution of supergene families associated with
insecticide resistance.
Ranson, H., Claudianos, C., Ortelli, F., Abgrall, C., Hemingway, J.
Sharakhova, M.V., Unger, M.F., Collins, F.H. and Feyereisen, R.
Science 298, 179-181 (2002)
Anopheles map name CYPl3r3
CYP12F3 Anopheles gambiae (malaria vector)
No accession number
Submitted by Rene Feyereisen July 5, 2002
Low 40% range with other CYP12s 60% to 12F2
CYP12F3 Anopheles gambiae (malaria mosquito)
GenEMBL
Submitted by Christelle Abgrall, Hilary Ranson and Rene Feyereisen
See the paper: Evolution of supergene families associated with
insecticide resistance.
Ranson, H., Claudianos, C., Ortelli, F., Abgrall, C., Hemingway, J.
Sharakhova, M.V., Unger, M.F., Collins, F.H. and Feyereisen, R.
Science 298, 179-181 (2002)
Anopheles map name CYPl3r2
CYP12F4 Anopheles gambiae (malaria vector)
No accession number
Submitted by Rene Feyereisen July 5, 2002
Low 40% range with other CYP12s 55% to 12F2
CYP12F4 Anopheles gambiae (malaria mosquito)
GenEMBL
Submitted by Christelle Abgrall, Hilary Ranson and Rene Feyereisen
See the paper: Evolution of supergene families associated with
insecticide resistance.
Ranson, H., Claudianos, C., Ortelli, F., Abgrall, C., Hemingway, J.
Sharakhova, M.V., Unger, M.F., Collins, F.H. and Feyereisen, R.
Science 298, 179-181 (2002)
Anopheles map name CYPl3r1
CYP12F5 Aedes aegypti (yellow fever mosquito)
CYP12F6 Aedes aegypti (yellow fever mosquito)
CYP12F7 Aedes aegypti (yellow fever mosquito)
CYP12F8 Aedes aegypti (yellow fever mosquito)
CYP12F9 Culex_quinquefasciatus (southern house mosquito)
No accession number
Osamu Komagata
submitted to nomenclature committee 7/25/07
sequence 15
68% to 12F7 Aedes, 60% to 12F2
CYP12G1 Lucilia cuprina (Australian blowfly)
No accession number
Zhenzhong Chen
Submitted to nomenclature committee March 17, 2005
Clone name Lc_CYP12C
51% to 12C1 (310 aa)
CYP12H1 Tribolium castaneum (red flour beetle)
GenEMBL XP_966937, DT790363.1 EST
37% to 12c1, 38% to 12A4 mito clan, 42% to 12F2
MSTGSENVISQKKLDHYKNLNALENSDVMPEGWDKAKPFESLPGPKPIPFFGN
IWRFLVPKIGDFYGIDFLDLHKIFYEQYGDISILKGMINDPIVVIFNPKDYETLFRNEGIWPIRKGLQSF
NEYRKSRKDIFVNAGLLLEEGENWFKIRSVVNPILMQPRNISQYTDKMNSVADELVNNITNLLEESENGE
MPENFHNELYKWSLESVGLVTLDTHLGCLKNDLDEDSEPQKLIRSTLEMFKLMHKLDVLPSFHNYVSTPS
WRKFVQVMDFIVETNMKYVNQVLDKLEKGETTSTEIPSVLEKLLKVDRNIAVTMSIDMMIAGIDTTGRIL
GAALYFLGKNHEAQEKLRSEAISLLQTKDQAVTSEVLKKAPYLKAVIKETTRLAPIGIGNLRTTVKNLVL
GGYQIPKGTDVVTSNLVLCTNDEYFSRAKEFIPERWLSTTSGELSKKNTNPFIFAPFGYGPRSCVGKRLA
SLELEVALLKIIRNFELDWPHEDMVFKTKMLYGMTEPLKIHVRSV
CYP12J1 Leptinotarsa decemlineata (Colorado potato beetle)
No accession number
Jianhua Zhang
Submitted to nomenclature committee April 2, 2006
Clone name P12F5113
59 amino acids from heme to end
42% to 12A4, 57% to CYP12H1 XP_966937
5 aa diffs to CYP12J2
58% to XM_961844.1 PREDICTED: Tribolium castaneum
37% to 12c1, 38% to 12A4, 42% to 12f2
CYP12J2 Leptinotarsa decemlineata (Colorado potato beetle)
No accession number
Jianhua Zhang
Submitted to nomenclature committee April 2, 2006
Clone name P12F231134
59 amino acids from heme to end
43% to 12A4, 57% to CYP12H1 XP_966937 91% to 12J1
CYP12J3 Ips pini (pine engraver, bark beetle)
No accession number
Pamela Sandstrom
Submitted to nomenclature committee on April 26, 2006
Full length sequence
60% to 12J1 Colorado potato beetle (C-term piece)
44% to 12H1 Tribolium castaneum
clone name EST 28H12
CYP12K1 Nasonia vitripennis (jewel wasp)
See wasp page
CYP12L1 Lucilia cuprina (Australian blowfly)
No accession number
Zhenzhong Chen
Submitted to nomenclature committee Dec. 18, 2007
clone name Luce0117K18
46% identical to M. domestica CYP12A2
13A Subfamily
CYP13A1 Caenorhabditis elegans (nematode worm)
GenEMBL Z48717 (34881 bp) and Z92859 (Y53C12)
Wilson,R., Ainscough,R., Anderson,K., Baynes,C., Berks,M.,
Bonfield,J., Burton,J., Connell,M., Copsey,T., Cooper,J.,
Coulson,A., Craxton,M., Dear,S., Du,Z., Durbin,R., Favello,A.,
Fulton,L., Gardner,A., Green,P., Hawkins,T., Hillier,L., Jier,M.,
Johnston,L., Jones,M., Kershaw,J., Kirsten,J., Laister,N.,
Latreille,P., Lightning,J., Lloyd,C., McMurray,A., Mortimore,B.,
O'Callaghan,M., Parsons,J., Percy,C., Rifken,L., Roopra,A.,
Saunders,D., Shownkeen,R., Smaldon,N., Smith,A., Sonnhammer,E.,
Staden,R., Sulston,J., Thierry-Mieg,J., Thomas,K., Vaudin,M.,
Vaughan,K., Waterston,R., Watson,A., Weinstock,L.,
Wilkinson-Sproat,J. and Wohldman,P.
2.2 Mb of contiguous nucleotide sequence from chromosome III of C.
elegans.
Nature 368, 32-38 (1994)
Product T10B9.8
CYP13A2 Caenorhabditis elegans (nematode worm)
GenEMBL Z48717 (34881 bp) and Z92859 (Y53C12)
see CYP13A1 for reference.
Product T10B9.7
CYP13A3 Caenorhabditis elegans (nematode worm)
GenEMBL Z48717 (34881 bp) and Z92859 (Y53C12)
see CYP13A1 for reference.
Product T10B9.5
CYP13A4 Caenorhabditis elegans (nematode worm)
GenEMBL Z48717 (34881 bp) and Z92859 (Y53C12)
see CYP13A1 for reference.
Product T10B9.1
CYP13A5 Caenorhabditis elegans (nematode worm)
GenEMBL Z48717 (34881 bp) and Z92859 (Y53C12)
see CYP13A1 for reference.
Product T10B9.2
CYP13A6 Caenorhabditis elegans (nematode worm)
GenEMBL Z48717 (34881 bp) and Z92859 (Y53C12)
see CYP13A1 for reference.
Product T10B9.3
CYP13A7 Caenorhabditis elegans (nematode worm)
GenEMBL Z48717 (34881 bp) and Z92859 (Y53C12)
see CYP13A1 for reference.
Product T10B9.10
CYP13A8 Caenorhabditis elegans (nematode worm)
GenEMBL Z48717 (34881 bp) and Z83130 (ZK1325) and Z92859 (Y53C12)
see CYP13A1 for reference.
Product T10B9.4
CYP13A9P Caenorhabditis elegans (nematode worm)
GenEMBL Z48717 (34881 bp) and Z92859 (Y53C12)
see CYP13A1 for reference.
Product T10B9.6 pseudogene
Note: closer inspection suggests this may not be a pseudogene.
A complete gene can be assembled, but perhaps the genefinder program
did not see
it.
CYP13A10 Caenorhabditis elegans (nematode worm)
GenEMBL Z46934 (35989 bp) and Z92859 (Y53C12)
see CYP13A1 for reference.
product ZK1320.4
CYP13A11 Caenorhabditis elegans (nematode worm)
GenEMBL Z81503 (F14F7) and Z92819 (Y37D8) and Z94158 (Y39E4)
F14F7.a contig 666 10-13k region
CYP13A12 Caenorhabditis elegans (nematode worm)
GenEMBL Z81503 (F14F7) and Z92819 (Y37D8) and Z94158 (Y39E4)
F14F7.b contig 666 13-18k region
13B Subfamily
CYP13B1 Caenorhabditis elegans (nematode worm)
GenEMBL Z54269 (19692bp) and Z92827 (C29F7)
F02C12.5 also C29F7 has the end of this sequence
Formerly CYP16A1 but renamed after full length sequence sorted with
CYP13
CYP13B2 Caenorhabditis elegans (nematode worm)
GenEMBL Z81565
K06G5
14A Subfamily
CYP14A1 Caenorhabditis elegans (nematode worm)
GenEMBL Z50742 (20856bp)
Wilson,R., Ainscough,R., Anderson,K., Baynes,C., Berks,M.,
Bonfield,J., Burton,J., Connell,M., Copsey,T., Cooper,J.,
Coulson,A., Craxton,M., Dear,S., Du,Z., Durbin,R., Favello,A.,
Fulton,L., Gardner,A., Green,P., Hawkins,T., Hillier,L., Jier,M.,
Johnston,L., Jones,M., Kershaw,J., Kirsten,J., Laister,N.,
Latreille,P., Lightning,J., Lloyd,C., McMurray,A., Mortimore,B.,
O'Callaghan,M., Parsons,J., Percy,C., Rifken,L., Roopra,A.,
Saunders,D., Shownkeen,R., Smaldon,N., Smith,A., Sonnhammer,E.,
Staden,R., Sulston,J., Thierry-Mieg,J., Thomas,K., Vaudin,M.,
Vaughan,K., Waterston,R., Watson,A., Weinstock,L.,
Wilkinson-Sproat,J. and Wohldman,P.
2.2 Mb of contiguous nucleotide sequence from chromosome III of C.
elegans.
Nature 368 (6466), 32-38 (1994)
K09A11.2
CYP14A2 Caenorhabditis elegans (nematode worm)
GenEMBL Z50742 (20856bp)
see CYP14A1 for reference.
K09A11.3
CYP14A3 Caenorhabditis elegans (nematode worm)
GenEMBL Z50742 (20856bp)
see CYP14A1 for reference.
K09A11.4
CYP14A4 Caenorhabditis elegans (nematode worm)
GenEMBL Z50742 (20856bp) Z70212(R04D3.1 continuation of Z50742)
see CYP14A1 for reference.
after K09A11.5
partial sequence at end of cosmid continues on cosmid R04D3
CYP14A5 Caenorhabditis elegans (nematode worm)
GenEMBL U64847
F08F3.7
CYP15A1 Diploptera punctata (cockroach)
GenEMBL AY509244
Helvig,C., Koener,J.F., Unnithan,G.C. and Feyereisen,R.
CYP15A1, the cytochrome P450 that catalyzes epoxidation of methyl
farnesoate to juvenile hormone III in cockroach corpora allata
Unpublished
CYP15A1 Tribolium castaneum (red flour beetle)
GenEMBL XP_970303
44% to CYP15B1 Anopheles, 48% to CYP15B1 Aedes
note: CYP15A1, B1 and C1 are all probably orthologs.
Each species seems to have only a single CYP15.
MLFFVTLVISLVLLFLILDTIKPRRYPPGPKWLPIVGNFLEFRRRLSEIGYHHLVWKEFSEEYGDVVGLK
MGRNLVVAVFGAEAVKEVLTREEFDGRPDGFFFRLRTFGKRLGIVFSDGQFWQKQRKFSMQHLRNFGFGR
KEMEEKIEEETKDLIAVFKKQCSEPIWMHTAFDVSVLNVLWAMMAGERFNINDERLRKLLKIVHDAFRLT
DMSGGMLNQLPFLRFIAPETCGYNQLVDVLVRMWEFLQETISEHRKTLCSSHARDLIDAFLQKMDIQSDS
SFTDDQLMSLCLDLFMAGSETTSNTLGFSVVYMLQFPEVQKKVQDEMDEVVGRNRWPTLQDRIKLKYTEA
VLMEIQRRANIPPLGIAHRATRDVSLFDYRIPEGTIVLTSLYSVHMDHKFWKDPLAFRPERFLNKEGNLE
VDEKYFAPFGYGKRRCLGESLAKANYFLFFTALLHNFYLEKDCDGPEPQLEGYDGVTISPKPFRAKLIPRTD
CYP15A1 Pediculus humanus humanus (body louse)
No accession number
Reed Johnson and Barry Pittendrigh
Submitted to nomenclature committee March 14, 2007
Ph15, 49% to 15A1 Tribolium
Pediculus genome site
CYP15A1 Nasonia vitripennis (jewel wasp)
See wasp page
CYP15B1 Anopheles gambiae (malaria mosquito)
GenEMBL
Submitted by Christelle Abgrall, Hilary Ranson and Rene Feyereisen
See the paper: Evolution of supergene families associated with
insecticide resistance.
Ranson, H., Claudianos, C., Ortelli, F., Abgrall, C., Hemingway, J.
Sharakhova, M.V., Unger, M.F., Collins, F.H. and Feyereisen, R.
Science 298, 179-181 (2002)
Anopheles map name CYPj2l3
47% identical to 15A1, though this is probably the ortholog of CYP15A1
CYP15B1 Aedes aegypti (yellow fever mosquito)
CYP15B1 Culex pipiens quinquefasciatus (southern house mosquito)
XM_001864994
locus_tag="CpipJ_CPIJ014944
72% to CYP15B1 Aedes aegypti
MRKPAFFPPGPAWFPLIGCGYRVFKLVNVHKFYHLMWDALARRY
GPVVGLKLGRDRVVIVSGLAAIREFYSKEAFNGRPNGFFFRIRSFNKRLGLVFNDGAD
WDVQRRFSVKTLKSLGMGRTGMVHSLEKETEELIHHLRKLSRGGRTIQMHSAFDIAVL
NVIWTLLAGNRFKLDDKRLAWICDTIHNSFRVIDMSGGVLNQCPPIRHICPNKSGFKP
LVNLLNPLLNFINDTISNIKANPECPERHNCLISSYLEELQIDNPHPSFSNEQLLSLC
LDLFQAGSETTSNTLGFGVAHVMHHPNVIAKIHQELDNVVGRYRLPTLDDRPSLPYLE
ATICEIQRIANVAPLAIAHRTTSPVQLGQYVIPRDTIAMVSLYSIHMDRQYWGDPEVF
RPERFLNESEDKLIQHEYFLPFGAGKRRCLGEALAKSNLFLFFAAFMHAFIVEPANPG
QLPPLEGFDGITLSPQPYQVRLKERLI
CYP15C1 Bombyx mori (silkworm)
BAAB01071346.1 BAAB01157546.1 BAAB01036905.1 BAAB01022841.1
BAAB01157214.1BAAB01068330.1
40% to CYP15
note: CYP15A1, B1 and C1 are all probably orthologs. Each species
seems to only have a single CYP15. (not found in Drosophila)
See silkworm page for sequence
CYP15D1X Daphnia pulex (water flea)
Confidential
renamed CYP370A1
CYP15D2X Daphnia pulex (water flea)
Confidential
renamed CYP370A2
CYP15D3PX Daphnia pulex (water flea)
Confidential
renamed CYP370A3P
CYP15D4X Daphnia pulex (water flea)
Confidential
renamed CYP370A4
CYP15D5X Daphnia pulex (water flea)
Confidential
renamed CYP370A5
CYP15D6X Daphnia pulex (water flea)
Confidential
renamed CYP370A6
CYP15D7X Daphnia pulex (water flea)
Confidential
renamed CYP370A7
CYP15D8X Daphnia pulex (water flea)
Confidential
renamed CYP370A8
CYP15D9X Daphnia pulex (water flea)
Confidential
renamed CYP370A9
CYP15D10X Daphnia pulex (water flea)
Confidential
renamed CYP370A10
CYP15D11X Daphnia pulex (water flea)
Confidential
renamed CYP370A11
CYP15D12X Daphnia pulex (water flea)
Confidential
renamed CYP370A12
CYP15D13X Daphnia pulex (water flea)
Confidential
renamed CYP370A13
CYP15E1X Daphnia pulex (water flea)
Confidential
renamed CYP370B1
CYP15E2X Daphnia pulex (water flea)
Confidential
renamed CYP370B2
16A Subfamily
CYP16A1X Caenorhabditis elegans (nematode worm)
GenEMBL Z54269
number retired
This sequence is now CYP13B1
17A Subfamily
CYP17 human
GenEMBL M14564
Chung,B.C., Picado-Leonard,J., Haniu,M., Bienkowski,M., Hall,P.F.,
Shively,J.E. and Miller,W.L. (1987)
Cytochrome P450c17 (steroid 17 alpha-hydroxylase/17,20 lyase):
cloning of human adrenal and testis cDNAs indicates the same gene
is expressed in both tissues
Proc. Natl. Acad. Sci. U.S.A. 84, 407-411.
First human CYP17A1 cDNA
CYP17 human
GenEMBL M19489
Picado-Leonard J, Miller WL. (1987) Cloning and sequence of the human
gene for P450c17 (steroid 17 alpha-hydroxylase/17,20 lyase):
similarity with the gene for P450c21.
DNA. 6. 439-448.
First human CYP17A1 gene
CYP17 human
GenEMBL NM_000102
MWELVALLLLTLAYLFWPKRRCPGAKYPKSLLSLPLVGSLPFLP
RHGHMHNNFFKLQKKYGPIYSVRMGTKTTVIVGHHQLAKEVLIKKGKDFSGRPQMATL
DIASNNRKGIAFADSGAHWQLHRRLAMATFALFKDGDQKLEKIICQEISTLCDMLATH
NGQSIDISFPVFVAVTNVISLICFNTSYKNGDPELNVIQNYNEGIIDNLSKDSLVDLV
PWLKIFPNKTLEKLKSHVKIRNDLLNKILENYKEKFRSDSITNMLDTLMQAKMNSDNG
NAGPDQDSELLSDNHILTTIGDIFGAGVETTTSVVKWTLAFLLHNPQVKKKLYEEIDQ
NVGFSRTPTISDRNRLLLLEATIREVLRLRPVAPMLIPHKANVDSSIGEFAVDKGTEV
IINLWALHHNEKEWHQPDQFMPERFLNPAGTQLISPSVSYLPFGAGPRSCIGEILARQ
ELFLIMAWLLQRFDLEVPDDGQLPSLEGIPKVVFLIDSFKVKIKVRQAWREAQAEGST
CYP17 human EST
GenEMBL Z19875 (235bp)
UK-HGMP (United Kingdom human genome mapping project)
covers amino acids 270-348 when translating the complementary
strand. The fragment goes through at least 6 frame shifts.
sequence ID AAAAWEO
CYP17 human EST
GenEMBL Z20209 (248bp)
UK-HGMP (United Kingdom human genome mapping project)
covers amino acids 265-349 when translating the complementary
strand. The fragment goes through at least 8 frame shifts.
sequence ID AAABPSZ
CYP17 human
GenEMBL S85459 (556bp)
Biason,A., Mantero,F., Scaroni,C., Simpson,E.R. and Waterman,M.R.
Deletion within the CYP17 gene together with insertion of foreign
DNA is the cause of combined complete 17
alpha-hydroxylase/17,20-lyase deficiency in an Italian patient
Mol. Endocrinol. 5, 2037-2045 (1991)
CYP17A1 Macaca fasicularis (cynomolgus monkey)
No accession number
Yasuhiro Uno
Submitted to nomenclature committee 1/11/2005
Clone name mfCYP17A1
94% to human, missing first 9 aa
CYP17 rat
PIR A31359 (507 amino acids)
Namiki, M., Kitamura, M., Buczko, E. and Dufau, M.L.
Rat testis P-450-17-alpha cDNA: the deduced amino acid
sequence, expression and secondary structural configuration.
Biochem. Biophys. Res. Commun. 157, 705-712 (1988)
CYP17 rat
PIR D41425 (16 amino acids)
Imaoka, S., Kamataki, T. and Funae, Y.
Purification and characterization of six cytochromes P-450
from hepatic microsomes of immature female rats.
J. Biochem. 102, 843-851 (1987)
CYP17 rat
GenEMBL S50146 Z11902 (3345bp)
PIR S20655 (97 amino acids)
Nason,T.F., Han,X.G. and Hall,P.F.
Cyclic AMP regulates expression of the rat gene for steroid
17 alpha-hydroxylase/C17-20 lyase P-450 (CYP17) in rat Leydig
cells.
Biochim. Biophys Acta 1171, 73-80 (1992)
Note: have not been able to download S50146 from GCG or NCBI.
CYP17 rat
GenEMBL X69816 (7556bp)
Givens,C.R., Zhang,P., Bair,S.R. and Mellon,S.H.
Transcriptional regulation of rat cytochrome P450c17 expression in
mouse Leydig MA-10 and adrenal Y-1 cells: identification of a
single protein that mediates both basal and cAMP-induced activities.
DNA Cell Biol. 13, 1087-1098 (1994)
CYP17 rat
PIR S24316 (97 amino acids)
Zhang, P., Nason, T.F., Han, X.G. and Hall, P.F.
Gene for 17-alpha-hydroxylase/C(17-20) lyase P-450: complete
nucleotide sequence of the porcine gene and 5' upstream
sequence of the rat gene.
Biochim. Biophys. Acta 1131, 345-348 (1992)
Cyp17 mouse
genEMBL M64863 AC079419.1 Mm.1262
MWELVGLLLLILAYFFWPKSKTPNAKFPRSLPFLPLVGSLPFLP
RRGHMHANFFKLQEKYGPIYSLRLGTTTAVIVGHYQLAREVLVKKGKEFSGRPQMVTL
GLLSDQGKGVAFADSSSSWQLHRKLVFSTFSLFRDDQKLEKMICQEANSLCDLILTYD
GESRDLSTLIFKSVINIICTICFNISFENKDPILTTIQTFTEGIVDVLGHSDLVDIFP
WLKIFPNKNLEMIKEHTKIREKTLVEMFEKCKEKFNSESLSSLTDILIQAKMNAENNN
TGEGQDPSVFSDKHILVTVGDIFGAGIETTSSVLNWILAFLVHNPEVKRKIQKEIDQY
VGFSRTPSFNDRTHLLMLEATIREVLRIRPVAPLLIPHKANIDSSIGEFAIPKDTHVI
INLWALHHDKNEWDQPDRFMPERFLDPTGSHLITPTPSYLPFGAGPRSCIGEALARQE
LFIFMALLLQRFDFDVSDDKQLPCLVGDPKVVFLIDPFKVKITVRQAWKDAQVEVST
CYP17 hamster
no accession number
Cloutier,M., Fleury,A., Courtemanche,J., Ducharme,L.
Mason,J.I. and Lehoux,J.G.
Cloning and expression of hamster adrenal cytochrome P450c17 cDNA.
Ann. N.Y. Acad. Sci. 774, 294-296 (1995)
CYP17 Cavia (guinea pig)
GenEMBL S75277(1732bp)
Tremblay,Y., Fleury,A., Beaudoin,C., Vallee,M. and Belanger,A.
Molecular cloning and expression of guinea pig cytochrome P450c17
cDNA (steroid 17 alpha-hydroxylase/17,20 lyase): tissue
distribution, regulation, and substrate specificity of the
expressed enzyme.
DNA Cell Biol. 13 (12), 1199-1212 (1994)
CYP17 Cavia porcellus (guinea pig)
PIR S52756 (508 amino acids)
Huang,Y., Voigt,J.M. and Colby,H.D.
unpublished
CYP17 pig
GenEMBL Z11854 to Z11856 GenEMBL S40341 (1858bp)
PIR S24233 (501 amino acids) PIR S30074 (501 amino acids)
Zhang,P. Nason,T.F., Han,X.G. and Hall,P.F.
Gene for 17 alpha-hydroxylasae/C(17-20) lyase P-450: complete
nucleotide sequence of the porcine gene and 5' upstream sequence of
the rat gene.
Biochim. Biophys. Acta 1131, 345-348 (1992)
CYP17 Sus scrofa (pig)
GenEMBL U41519 to U41525
Conley,A.J., Graham-Lorence,S.E., Kagimoto,M., Lorence,M.C.,
Murry,B.A., Oka,K., Sanders,D. and Mason,J.I.
Nucleotide sequence of a cDNA encoding porcine testis 17
alpha-hydroxylase cytochrome P-450.
Biochim. Biophys. Acta 1130, 75-77 (1992)
MWVLLVFFLLTLTYLFWPKTKGSGAKYPRSLPVLPVVGSLPFLP
RRGHQHMNFFKLQDKYGPIFSFRLGSKTTVVIGDHQLAKEVLLKKGKEFSGRPRVMTL
DILSDNQKGIAFADHGTSWQLHRKLALSTFSLFKGGNLKLENIINQEIKVLCDFLATR
NGESIDLAQPLSLAMTNIVSFICFNFSFKKGDPALQAIVNFNDGILDAVGKEILYDMF
PGIRILPSQTLENMKQCVRMRNELLREILENRKENYSRNSITNLLDIMIQAKTNAESN
TGGPDHNLKLLSDRHMLATVADIFGAGVETSASVVKWIVAFLLHYPLLRKKIQDAIDQ
NIGFNRAPSISDRNQLVLLEATIREVLRFRPVSPTLIPHRAIIDSSIGEFTIDKDTDV
VVNLWALHHNEKEWHRPDLFMPERFLDPTGTQLISPSLSYLPFGAGPRSCVGEMLARQ
ELFLFTAGLLQRFDLELPDDGQLPCLVGNPSLVLQIDPFKVKIKERQAWKEAHTEGSTS
CYP17 bovine
GenEMBL M64646 (1725bp)
Zuber,M.X., John,M.E., Okamura,T., Simpson,E.R. and Waterman,M.R.
Bovine adrenocortical cytochrome P-450-17-alpha: Regulation of gene
expression by ACTH and elucidation of primary sequence
J. Biol. Chem. 261, 2475-2482 (1986)
CYP17A1 Bos taurus (cow)
See cattle page for details
MWLLLAVFLLTLAYLFWPKTKHS
116 GAKYPRSLPSLPLVGSLPFLPRRGQQHKNFFKLQEKYGPIYSFRLGSKTTVMIGHHQLAR 295
296 EVLLKKGKEFSGRPKVATLDILSDNQKGIAFADHGAHWQLHRKLALNAFALFKDG 460
461 NLKLEKIINQEANVLCDFLATQHGEAIDLSEPLSLAVTNIISFICFNFSFKNEDP 625
626 ALKAIQNVNDGILEVLSKEVLLDIFPVLKIFPSKAMEKMKGCVQTRNELLNEILEKCQEN 805
806 FSSDSITNLLHILIQAKVNADNNNAGPDQDSKLLSNRHMLATIGDIFGAGVETTTSVIK 982
983 WIVAYLLHHPSLKKRIQDDIDQIIGFNRTPTISDRNRLVLLEATIREVLRIRPVAPTLIP 1162
1163 HKAVIDSSIGDLTIDKGTDVVVNLWALHHSEKEWQHPDLFMPERFLDPTGTQLISPSL 1336
1337 SYLPFGAGPRSCVGEMLARQELFLFMSRLLQRFNLEIP 1450
DDGKLPSLEGHASLVLQIKPFKVKIEVRQAWKEAQAEGSTP*
CYP17 Ovis aries (sheep)
GenEMBL L40335 (1728bp)
Murry,B.A., Swart,P. and Mason,J.I.
Cloning and expression of ovine cytochrome P-450 17-alpha
hydroxylase/c17-20 lyase.
Unpublished (1995)
CYP17 Equus caballus (horse)
GenEMBL D30688(1906bp) D13818
Hasegawa,T., Mukoyama,H., Yoshida,S. and Takahashi,M.
Molecular cloning and nucleotide sequence of equine testicular
cytochrome P-450 steroid 17alpha-hydroxylase/C17,20-lyase messenger
ribonucleic acid.
Biol. Reprod. Mono. 1, 615-622 (1995)
CYP17 Equus caballus (horse)
GenEMBL D88184(6217bp)
Hasegawa,T.
Exon/Intron structure of Equine P450c17.
unpublished (1996)
CYP17A1 Xenopus tropicalis
See Xenopus pages for seq
CYP17A1 Danio rerio (zebrafish)
See xebrafish pages for seq
CYP17 Oncorhynchus mykiss (rainbow trout)
GenEMBL S50356
Sakai,N., Tanaka,M., Adachi,S., Miller,W.L. and Nagahama,Y.
Rainbow trout cytochrome P-450c17 (17 alpha-hydroxylase/17-20
lyase) cDNA cloning, enzymatic properties and temporal pattern of
ovarian P-450c17 mRNA expression during oogenesis.
FEBS Lett. 301, 60-64 (1992)
Identical to X65800
CYP17 Oncorhynchus mykiss (rainbow trout)
GenEMBL X65800 (2287bp) Swiss P30437 (514 amino acids)
Sakai,N., Tanaka,M., Adachi,S., Miller,W.L. and Nagahama,Y.
Rainbow trout cytochrome P-450c17 (17
alpha-hydroxylase/17,20-lyase). cDNA cloning, enzymatic properties
and temporal pattern of ovarian P-450c17 mRNA expression during
oogenesis
FEBS Lett. 301, 60-64 (1992)
Identical to S50356
CYP17 Oryzias latipes (medaka)
GenEMBL D87121(2421bp)
Kobayashi,D., Matsuyama,M., Tanaka,M., Fukada,S. and Nagahama,Y.
Structural analysis of medaka P-450c17 and expression in the
ovarian follicle.
unpublished (1996)
CYP17 Oryzias latipes (medaka)
GenEMBL D87122(2302bp)
Kobayashi,D., Tanaka,M., Fukada,S. and Nagahama,Y.
Presence of a Novel Cytochrome P-450c17 Transcripts in Medaka
Gonads
unpublished (1996)
note: this sequence is missing exon 6
otherwise identical to D87121
CYP17 Squalus acanthias (dogfish)
GenEMBL S77384 (1964bp)
Trant,J.
Isolation and characterization of the cDNA encoding the spiny
dogfish shark
(Squalus acanthias) form of cytochrome P450c17
J. Exp. Zool. 272, 25-33 (1995)
CYP17 Ictalurus punctatus (channel catfish)
GenEMBL AF063837
Trant,J.M., Berard,C., Byrne,B.J. and Wunder,J.
Isolation and heterologous expression of the cDNA encoding the
cytochrome P450 17-hydroxylase from the channel catfish (Ictalurus
punctatus)
Unpublished (1998)
CYP17A1 Fugu rubripes (pufferfish)
No accession number
Scaffold_4175
80% to Oryzias latipes 17, 79% to trout 17 73% to catfish
61% to dogfish 48% to other CYP17 from Fugu
MDWVLFVYAFSAVNLALLALHLKFRTPASGPRGPPRLPALPLIGSLLSLRSPHPPHVLFKE
LQGKYGQTYSLMMGSHRVIIVNHHAHAKEVLLKKGKIFAGRPRS 11667 (0)
11572 VTTDVLSRDGKDIAFGDYSATWRFHRKIVHGALCMFGEGSASIEKI 11435 (1)
10871 ICAEAASLCSILSEAWTAGLALDLSPELTRAVTNVICSLCFSSSYRRGDAEFEAMLHYSQ 10692
10691 GIVDTVAKDSLVDIFPCLQ 10635 (0)
IFPNADLRLLKRCVSVRDKLLQKEYDKHK (0)
AAYSDHVQRDLLDALLRAKCSAENNNTTGINAESVGLTDDHLLMT
VGDIFGAGVETTTTVMKWAITYLIHHPQ 9765 (0)
9637 IQSRIQEELDSRVGMDRSPQLSDRGSLPYLEATIREVLRIRPVAPLFIPHVALSDT 9473 (2)
9369 SIGDFAVKKGTRVVINLWSLHHDEKEWENPERFDP 9265 (1)
GRFLNSEGTGLVIPSSSYLPFGAGVRVCLGEALAK 8558
8557 MELFLFLSWILQRFTLTVPSGHSLPSLEGKFGVVLQPTKYKVNATPRPGWEGKCKACWN* 8378
CYP17A2 Danio rerio (zebrafish)
See xebrafish pages for seq
CYP17A2 Fugu rubripes (pufferfish)
No accession number
Scaffold_8086
50% to Oryzias latipes 17, 48% to trout 17 50% to catfish
49% to dogfish 48% to other CYP17 from Fugu
1198 MVTVGSFLIFRRPVRGSEPGSEAGPPRVKVPCISWVPVLGSLPWLRGGRPLHLIFTQLSYR 1380 (2)
1600 YGPLFALYLGPHLTVVVNNHQHAREVLLLRGKDFAGRPRM 1719 (0)
1797 VTTDLLTRGGKDIAFSDYCPLWKSHRRLVQNSFTLFGEGTSRLQDM 1934 (1)
2061 VLAAVDSLCEELLSMEGRGFDPAPAVTRAVTNVVCMLVFSATYRHGDSELQEVLRYNDGI 2240
2241 VQTIAGGGLVDIYPWMK 2291 (0)
2372 VFPNKTLSKLKACIAVRDRLLTHKLEEHK 2458 (0)
2622 ATLTDNQPRDLLDALLMGQVGRGRRKGSGRVEEDIITEDHVLMTAAEAFGAGVETTSTTLLWILAYLLHHPQ 2837 (0)
2925 VQERVQKELDDHVGSERPVRVSDRARLTYLDCVINEGMRIRPVSPVLIPHTAMTDSR 3074 (2)
3870 IGGHHISRGTRVLVNMWSIHHDSAHWDKPDLFNP 3971 (1)
4519 DRFRDHQGQRVTPSCFLPFGAGPRVCVGESLARLELFLFLSSLLQRMSFRLPNGA 4692
4693 SPPDLQGRMGVVLQPVPYKVVVTPRVG* 4776
CYP17 fragment a Fugu rubripes (pufferfish)
No accession number
Fc:c028I22x2 LPC.10549.x2 Length = 1007 61% to 17A1 Fugu
GAPRATTDNHHAHAKEARPKKGKKAAGRPRK exon 1
ATTDGASRDGTDTAEGDHSATRRDQR exon 2
CYP17 fragment b Fugu rubripes (pufferfish)
No accession number
Fc:c028I22x1 LPC.10549.x1 Length = 894
78% to Fc:c028I22x2 65% to 17A1 Fugu
KEQQAEHGQTTSRMMGSHRGNTDNQHAHAKEARQKKGKKGAGRPRK exon 1
ATTDGRSRDGTDIAEGDHSATWRHHRKIVQRARRRNGEGSAPSEKI exon 2 C-helix
18A Subfamily
Cyp18a1 Drosophila melanogaster
GenEMBL S66112 (63bp)
Hurban,P. and Thummel,C.
Isolation and characterization of fifteen ecdysone-inducible
drosophila genes reveal unexpected complexities in ecdysone regulation.
Molec. Cell Biol. 13, 7101-7111 (1993)
Note: very short ecdysone inducible fragment in the heme binding
region about 2/3 of amino acids are identical to 2D sequences.
called Eig 17-1
dimethylnitrosamine demethylase
Cyp18a1 Drosophila melanogaster
GenEMBL U44753(2539bp)
Bassett,M.H, Waterman,M.R., McCarthy,J.L. and Sliter,T.J.
Cloning and characterization of CYP18 in Drosophila melanogaster:
identification of an insect member of a new cytochrome P450 family.
unpublished (1996)
complete sequence from the Eig 17-1 fragment
Cyp18a1 Drosophila melanogaster
GenEMBL AC012164 114600-117669 also AC015216
Cyp18a1 Drosophila pseudoobscura
See D. pseudoobscura page on this server for sequence
Obtained from UC Santa Cruz Browser
CYP18A1 Spodoptera littoralis (cotton leafworm)
No accession number
Lyndsay Davies
Submitted to nomenclature committee 2/22/02
61% identical to CYP18 from Drosophila
since there is only one CYP18 seq in Drosophila this new sequence
will be called CYP18 without a subfamily or number like CYP18A1.
If more CYP18s are found in a single species this may have to change.
CYP18A1 Apis mellifera (honeybee)
WGS section genbank
Submitted by May Berenbaum 2/11/04
AmGroup13.1a
60% to 18A1 Drosophila melanogaster.
CYP18A1 Bombyx mori (silkworm)
AU005208 EST BAAB01190855.1 BAAB01187772.1
60% to CYP18A1 Drosophila
See silkworm page for sequence
CYP18A1 Tribolium castaneum (red flour beetle)
GenEMBL XP_968393
58% to 18A1 Drosophila melanogaster
MFVYSGLVLWNFLAEELSTKVLAVFLMVLFLVRLVQMLVQMLKEASALPPGPWGLPILGSLPFLKGDLHL
HFRDLTHKYGSLISTRLGSQLIVVLSDYKMIRDAFRKEEFTGRPITEFTTLLDGYGVINTAGKLWKDQRR
FLHDGLRHFGMSYIGSRKTQMENRIMREVEEFLSVLTARKDTPIDLNPVLAVSLSNVICDILMSVRFSHN
DERFKRFMFLIDEGFKLFSSLEASFFIPILKYLPGQRQTREKIAKNRAEMAQFLQETIEEHRKSFDPSHL
RDLLDTYLYEIQKADEEGTGDHLFEGKDHDRQMQQIMGDLFSAGMETIKSSLQWAVLFMLHHPEVMKAVQ
EELDQVVGRRRLPKLEDLPYLPVTESTMLEVLRISSIVPMGTTHAPTRDLKLNGFHLPRHAQIVPLLHSV
HMDPSLWHEPERFNPSRFINAEGKVVKPEYFLPFGVGRRMCLGEILARMEIFSFFSSLLHSFDICVPTGE
TLPSLKGVAGVTISPNAFRVCLKPRPMEWDSMGTIRPAGSH
CYP18A1 Aedes aegypti (yellow fever mosquito)
CYP18A1 Pediculus humanus humanus (body louse)
No accession number
Reed Johnson and Barry Pittendrigh
Submitted to nomenclature committee March 14, 2007
Ph18, 56% to 18A1 Apis
Pediculus genome site
CYP18A1 Nasonia vitripennis (jewel wasp)
See wasp page
CYP18A1 Daphnia pulex (water flea)
Confidential
CYP18B1 Bombyx mori (silkworm)
BAAB01081335.1 BAAB01007952.1
BAAB01142048.1 BAAB01138082.1 BAAB01045663.1
39% to CYP18A1 Bombyx
See silkworm page for sequence
19A Subfamily
CYP19 human
GenEMBL S52034 (142bp) S52789 (106bp) S52793 (149bp)
S52794 (125bp)
Harada,N.
A unique aromatase (P-450AROM) mRNA formed by alternative
use of tissue-specific exons 1 in human skin fibroblasts.
Biochem. Biophys. Res. Commun. 189, 1001-1007 (1992)
CYP19 human
GenEMBL D14473 (295bp) S59092 S59095 S59171
Toda,K and Shizuta,Y.
Molecular cloning of a cDNA showing alternative splicing of
the 5'-untranslated sequence of mRNA for human aromatase P-450.
Eur. J. Biochem. 213, 383-389 (1993)
CYP19 human
GenEMBL D13391 (2238bp)
Katsumi,T. and Shizuta,Y.
Identification and characterization of cis-acting regulatory
elements for
the expression of the human aromatase cytochrome P-450 gene.
J. Biol. Chem. 269, 8099-8107 (1994)
CYP19 human
GenEMBL D21240 (794bp) D21241 (3231bp)
Harada,N., Utsumi,T. and Takagi,Y.
Tissue-specific expression of the human aromatase cytochrome P-450
gene by alternative use of multiple exons 1 and promoters, and
switching of tissue-specific exons 1 in carcinogenesis.
Proc. Natl. Acad. Sci. U.S.A. 90 (23), 11312-11316 (1993)
CYP19 human
GenEMBL X55983 (669bp)
Toda,K., Miyahara,K., Kawamoto,T., Ikeda,H., Sagara,Y. and
Shizuta,Y.
Characterization of a cis-acting regulatory element involved in
human-
aromatase P-450 gene expression.
Eur, J. Biochem. 205, 303-309 (1992)
exon 1
CYP19 human
GenEMBL S71536 (792bp)
Toda,K., Simpson,E.R., Mendelson,C.R., Shizuta,Y. and Kilgore,M.W.
Expression of the gene encoding aromatase cytochrome P450 (CYP19)
in fetal tissues
Mol. Endocrinol. 8, 210-217 (1994)
CYP19 human
GenEMBL M32245 (840bp)
Harada,N., Yamada,K., Saito,K., Kibe,N., Dohmae,S. and Takagi,Y.
Structural characterization of the human estrogen synthetase
(aromatase) gene.
Biochem. Biophys. Res. Commun. 166, 365-372 (1990)
CYP19 human
GenEMBL D29757 (875bp) PIR PC2041 (45 amino acids)
Honda,S.-I., Harada,N. and Takagi,Y.
Novel exon 1 of the aromatase gene specific for aromatase
transcripts
in human brain.
Biochem. Biophys. Res. Commun. 198, 1153-1160 (1994)
CYP19 human
GenEMBL M22246 (2966bp)
Harada,N.
Cloning of a complete cDNA encoding human aromatase: immunochemical
identification and sequence analysis
Biochem. Biophys. Res. Commun. 156, 725-732 (1988)
CYP19 human
GenEMBL L21982 (1166bp)
Mahendroo,M.S., Mendelson,C.R. and Simpson,E.R.
Tissue-specific and hormonally-controlled alternative promoters
regulate aromatase cytochrome P450 gene expression in human adipose
tissue
J. Biol. Chem. 268, 19463-19470 (1993)
CYP19 human
GenEMBL S85356 (1384bp)
Means,G.D., Kilgore,M.W., Mahendroo,M.S., Mendelson,C.R. and
Simpson,E.R.
Tissue-specific promoters regulate aromatase cytochrome P450 gene
expression in human ovary and fetal tissues.
Mol. Endocrinol. 5, 2005-2013 (1991)
CYP19 human
GenEMBL S96437 (971bp)
Kilgore,M.W., Means,G.D., Mendelson,C.R. and Simpson,E.R.
Alternative promotion of aromatase P-450 expression in the human
placenta.
Mol. Cell. Endocrinol. 83, R9-R16 (1992)
CYP19 human
PIR A40542 (48 amino acids)
Mahendroo, M.S., Means, G.D., Mendelson, C.R. and Simpson, E.R.
Tissue-specific expression of human P-450-AROM. The promoter
responsible for expression in adipose tissue is different
from that utilized in placenta.
J. Biol. Chem. 266, 11276-11281 (1991)
CYP19 Macaca fuscata (Japanese macaque)
GenEMBL S79807(369bp)
Yamada-Mouri,N., Hirata,S., Hayashi,M. and Kato,J.
Analysis of the expression and the first exon of aromatase mRNA in
monkey brain.
J. Steroid Biochem. Mol. Biol. 55 (1), 17-23 (1995)
CYP19 rat
GenEMBL S59505 (639bp)
Fitzpatrick,S.L. and Richards,J.S.
cis-acting elements of the rat aromatase promoter required for
adenosine 3',5'-monophosphate induction in ovarian granulosa cells
and constitutive expression in R2C Leydig cells.
Molec. Endocrinol. 7, 341-354 (1993)
Note: promoter
CYP19 rat
GenEMBL Z11815 (590bp)
Hickey,G.J., Krasnow,J.S., Beattie,W.G. and Richards,J.S.
Aromatase cytochrome P450 in rat ovarian granulosa cells before and
after luteinization: Adenosine 3',5'-monophosphate-dependent and
independent regulation. Cloning and sequencing of rat aromatase
cDNA and 5' genomic DNA
Mol. Endocrinol. 4, 3-12 (1990)
CYP19 Oryctolagus cuniculus (rabbit)
GenEMBL Z68271(1783bp)
Delarue,B., Mittre,H., Feral,C., Benhaim,A. and Leymarie,P.
Rapid sequencing of rabbit aromatase cDNA using RACE PCR without
cloning.
C. R. Acad. Sci. III, Sci. Vie 319, 663-670 (1996)
CYP19 Oryctolagus cuniculus (rabbit)
GenEMBL Z70302(1455bp)
Delarue,B., Mittre,H. and Leymarie,P.
Expression des transcrits codant pour l'aromatase de lapin dans
differents tissus.
unpublished (1996)
Cyp19 mouse
Swiss P28649 (503 amino acids) GenEMBL D00659 (2420bp)
Terashima,M., Toda,K., Kawamoto,T., Kuribayashi,I., Ogawa,Y.,
Maeda,T. and Shizuta,Y.
Isolation of a full-length cDNA encoding mouse aromatase P450
Arch. Biochem. Biophys. 285, 231-237 (1991)
MFLEMLNPMQYNVTIMVPETVTVSAMPLLLIMGLLLLIWNCESS
SSIPGPGYCLGIGPLISHGRFLWMGIGSACNYYNKMYGEFMRVWISGEETLIISKSSS
MFHVMKHSHYISRFGSKRGLQCIGMHENGIIFNNNPSLWRTIRPFFMKALTGPGLVRM
VEVCVESIKQHLDRLGEVTDTSGYVDVLTLMRHIMLDTSNMLFLGIPLDESAIVKKIQ
GYFNAWQALLIKPNIFFKISWLYRKYERSVKDLKDEIAVLVEKKRHKVSTAEKLEDCM
DFATDLIFAERRGDLTKENVNQCILEMLIAAPDTMSVTLYFMLLLVAEYPEVEAAILK
EIHTVVGDRDIKIEDIQNLKVVENFINESMRYQPVVDLVMRRALEDDVIDGYPVKKGT
NIILNIGRMHRLEYFPKPNEFTLENFEKNVPYRYFQPFGFGPRGCAGKYIAMVMMKVV
LVTLLRRFQVKTLQKRCIENIPKKNDLSLHPNEDRHLVEIIFSPRNSDKYLQQ
CYP19 pig
no accession number
Corbin, C.J., Khalil, M.W. and Conley, A.J.
Functional ovarian and placental isoforms od porcine aromatase.
Mol. Cell. Endocrinol. 113, 29-37 (1995)
CYP19 Sus scrofa (pig)
GenEMBL L15471 (454bp)
Ko,Y., Choi,I., Green,M.L., Simmen,F.A. and Simmen,R.C.
Transient expression of the cytochrome P450 aromatase gene in
elongating porcine blastocysts is correlated with uterine
insulin-like growth factor levels during peri-implantation
development.
Mol. Reprod. Dev. 37 (1), 1-11 (1994)
Note: This is only a fragment of 80 amino acids, including
helix K and the EXXR conserved sequence.
CYP19 Sus scrofa (pig)
GenEMBL U52141(1133bp)
Choi,I., Collante,W., Simmen,R.C.M. and Simmen,F.A.
Molecular cloning of multiple forms of cytochrome p450 aromatase
and their developmental expression in porcine blastocysts,
endometrium, and placenta.
Unpublished (1997)
CYP19 Sus scrofa (pig)
GenEMBL U52142(1584bp)
Choi,I., Collante,W., Simmen,R.C.M. and Simmen,F.A.
Molecular cloning of multiple forms of cytochrome p450 aromatase
and their developmental expression in porcine blastocysts,
endometrium, and placenta.
Unpublished (1997)
CYP19 Sus scrofa (pig)
GenEMBL U37309 (417bp)
Choi,I., Simmen,R.C. and Simmen,F.A.
Molecular cloning of cytochrome P450 aromatase complementary
deoxyribonucleic acid from periimplantation porcine and equine
blastocysts identifies multiple novel 5'-untranslated exons
expressed in embryos, endometrium, and placenta.
Endocrinology 137 (4), 1457-1467 (1996)
CYP19 Sus scrofa (pig)
GenEMBL U37311(2470bp)
Choi,I., Simmen,R.C. and Simmen,F.A.
Molecular cloning of cytochrome P450 aromatase complementary
deoxyribonucleic acid from periimplantation porcine and equine
blastocysts identifies multiple novel 5'-untranslated exons
expressed in embryos, endometrium, and placenta.
Endocrinology 137 (4), 1457-1467 (1996)
CYP19 Sus scrofa (pig)
GenEMBL U57510
Choi,I., Collante,W., Simmen,R.C.M., Troyer,D. and Simmen,F.A.
Molecular cloning and structural characterization of porcine
cytochrome p450 aromatase chromosomal genes: evidence for the
existence of multiple, closely related genes that encode
developmental and tissue-specific isoforms of aromatase.
unpublished (1997)
CYP19 Sus scrofa (pig)
GenEMBL U57517(287bp) U57518(287bp) U57519(358bp)
Choi,I., Collante,W., Simmen,R.C.M., Troyer,D. and Simmen,F.A.
Molecular cloning and structural characterization of porcine
cytochrome p450 aromatase chromosomal genes: evidence for the
existence of multiple, closely related genes that encode
developmental and tissue-specific isoforms of aromatase.
unpublished (1997)
CYP19 Sus scrofa (pig)
GenEMBL U57520(517bp) U57521(495bp)
Choi,I., Collante,W., Simmen,R.C.M., Troyer,D. and Simmen,F.A.
Molecular cloning and structural characterization of porcine
cytochrome p450 aromatase chromosomal genes: evidence for the
existence of multiple, closely related genes that encode
developmental and tissue-specific isoforms of aromatase.
unpublished (1997)
CYP19 bovine
GenEMBL S66248 (2104bp)
Hinshelwood,M.M., Corbin,C.J., Tsang,P.C. and Simpson,E.R.
Isolation and characterization of a cDNA insert encoding bovine
aromatase
cytochrome P450.
Endocrinology 133, 1971-1977 (1993)
Note two amino acid differences with Z32741
CYP19 bovine
GenEMBL M64646 (1725bp)
Zuber,M.X., John,M.E., Okamura,T., Simpson,E.R. and Waterman,M.R.
Bovine adrenocortical cytochrome P-450-17-alpha: Regulation of gene
expression by ACTH and elucidation of primary sequence.
J. Biol. Chem. 261, 2475-2482 (1986)
CYP19 bovine
GenEMBL Z32741 (4226bp) PIR S44210 (503 amino acids)
Vanselow,J. and Furbass,R.
Aromatase cytochrome P450 gene and pseudogene
unpublished (1994)
CYP19 bovine
GenEMBL Z69241 to Z69250 (genomic sequences)
Furbass,R. and Vanselow,J.
unpublished (1996)
CYP19P bovine
GenEMBL Z32813 (1006bp)
Vanselow,J. and Furbass,R.
Aromatase cytochrome P450 gene and pseudogene
unpublished (1994)
CYP19A1 Bos taurus (cow)
See cattle page for details
MLLEVLNPRHYNVTSMVSEVVPIASIAILLLTGFLLLVWNYEDTSSIPGPSYFLGIGPLI
SHCRFLWMGIGSACNYYNKMYGEFMRVWVCGEETLIISKSSSMFHVMKHSHYISRFGSKL
GLQFIGMHEKGIIFNNNPALWKAVRPFFTKALSGPGLVRMVTICADSITKHLDRLEEVCN
DLGYVDVLTLMRRIMLDTSNMLFLGIPLDESAIVVKIQGYFDAWQALLLKPDIFFKISWLCRKYEKSV
927 KDLKDAMEILIEEKRHRISTAEKLEDSIDFATELIFAEKRGELTRENVNQC 1079
1080 ILEMLIAAPDTMSVSVFFMLFLIAKHPQVEEAIIREIQTVVGERDIRIDDMQKLKVVEN 1256
1257 FINESMRYQPVVDLVMRKALEDDVIDGYPVKKGTNIILNLGRMHRLEFFPKPNEFTLE 1430
1431 NFAKNVPYRYFQPFGFGPRACAGKYITMVMMKVVLVTLLRR 1553
FHVQTLQGRCVEKMQKKNDLSLHPDETRDRLEMIFTPRNSDKCLER*
CYP19 Equus caballus (horse)
GenEMBL U37313 (458bp)
Choi,I., Simmen,R.C. and Simmen,F.A.
Molecular cloning of cytochrome P450 aromatase complementary
deoxyribonucleic acid from periimplantation porcine and equine
blastocysts identifies multiple novel 5'-untranslated exons
expressed in embryos, endometrium, and placenta.
Endocrinology 137 (4), 1457-1467 (1996)
CYP19 chicken (three different strains of chicken)
GenEMBL M73277 to M73285, M73286 to M73294
M73295 to M73303
PIR A41063 (495 amino acids)
Matsumine,H., Herbst,M., Ou,S.-H.I., Wilson,J.D. and
McPhaul,M.J.
Aromatase mRNA in the extragonadal tissues of chickens with
the henny-feathering trait is derived from a distinctive
promoter structure that contains a segment of a retroviral
long terminal repeat.
J. Biol. Chem. 266, 19900-19907 (1991)
CYP19 Coturnix coturnix japonica (Japanese quail)
GenEMBL S46949 (692bp) PIR A48977(230 amino acids)
Harada,N., Yamada,K., Foidart,A. and Balthazart,J.
Regulation of aromatase cytochrome P-450 (estrogen synthetase)
transcripts in the quail brain by testosterone.
Brain Res. Mol. Brain Res. 15, 19-26 (1992)
Note: only three amino acid differences with chicken.
CYP19 Coturnix coturnix (quail)
GenEMBL D50336(4351bp)
Kudo,T., Yamamoto,H., Sato,S. and Sutou,S.
Comparison of 5' upstream regions of chicken and quail aromatase
gene.
Unpublished (1995)
CYP19 Poephila guttata (zebra finch)
GenEMBL S75898(3188bp)
Shen,P., Campagnoni,C.W., Kampf,K., Schlinger,B.A., Arnold,A.P. and
Campagnoni,A.T.
Isolation and characterization of a zebra finch aromatase cDNA: in
situ hybridization reveals high aromatase expression in brain
Brain Res. Mol. Brain Res. 24 (1-4), 227-237 (1994)
CYP19A1 Xenopus tropicalis
See Xenopus pages for seq
CYP19 Ictalurus punctatus (channel catfish)
GenEMBL S75715(2102bp)
Trant,J.
Isolation and characterization of the cDNA encoding the channel
catfish (Ictalurus punctatus) form of cytochrome P450arom
Gen. Comp. Endocrinol. 95 (2), 155-168 (1994)
CYP19 Onchorynchus mykiss (rainbow trout)
Tanaka, M., Telecky, T.M., Fukada, S., Adachi,S., Chen, S. and
Nagahama, Y.
Cloning and sequence analysis of the cDNA encoding P-450 aromatase
(P450arom) from a rainbow trout (Onchorynchus mykiss) ovary,
relationship
between the amount of P450arom mRNA and the production of
oestradiol-17-beta
in the ovary.
J. Mol. Endocrin. 8, 53-61 (1992)
CYP19 Carassius auratus (goldfish)
GenEMBL U18974(2939bp)
Gelinas,D.M., Pitoc,G.A. and Callard,G.V.
Isolation of goldfish brain aromatase cDNA and analysis of
expression during the reproductive cycle and after steroid
treatment in vivo.
unpublished (1996)
CYP19 Oryzias latipes (medaka)
GenEMBL D82968(1851bp)
Tanaka,M., Fukada,S., Matsuyama,M. and Nagahama,Y.
Structure and promoter analysis of the cytochrome P-450 aromatase
gene of the teleost fish, medaka (Oryzias latipes)
J. Biochem. 117 (4), 719-725 (1995)
CYP19 Tilapia nilotica (Cichlid fish)
GenEMBL U72071(1804bp)
Chang,X.T., Kobayashi,T., Nakamura,M., Kajura,H. and Nagahama,Y.
Isolation and characterization of cDNA encoding the tilapia
(Oreochromis niloticus) cytochrome P450 aromatase (P450arom):
Changes in P450arom mRNA, protein and enzyme activity in ovarian
follicles during oogenesis.
J. Mol. Endocrinol. (1996) In press
CYP19 Haplochromis burtoni (cichlid fish)
GenEMBL AF114716
White,R.B.
Aromatase expression during social change in the cichlid fish,
Haplochromis burtoni.
Unpublished
CYP19 Paralichthys olivaceus (Japanese Flounder)
GenEMBL AB017182
Kitano,T., Takamune,K., Kobayashi,T., Nagahama,Y. and Abe,S.
Suppression of P450 Aromatase (P450arom) Gene Expression in
Sex-Reversed Males Produced by Rearing Genetically Female Larvae at
High Water Temperature during a period of Sex Differentiation in
Japanese Flounder (Paralichthys olivaceus)
Unpublished (1998)
CYP19A1 Fugu rubripes (pufferfish)
No accession number
Scaffold_7098
65% to AF183906 ovary form of CYP19 from Zebrafish
60% to AF183908 brain form of CYP19 from Zebrafish
61% to other Fugu CYP19
this is probably the ovary form
9466 MAAVGLDAEVLVSVSPNATEAESPGSSAGTRALIILTCLLLLVWSHTEKKSVP 9308 (1)
9242 SLLGPSFCLGFGPLLTYVRFIWTGIGTASNYYNKKYGDIVRVWVNGEETLVISR 9081 (2)
8985 ASAVHHVLKSRQYTSRFGSKQGLSCIGMNERGIIFNNNVTEWRKIRGYFTK 8830 (1)
8759 ALTGPAVQNTVEVCNSSTQAHLDRLEDLAQVDVLSLLRCTVVDISNRLFLDIPIN 8595 (1)
8499 EKELLLKIHKYFDTWQTVLIKPDIYFKFGWIHQKHKTAA 8392 (2)
8296 RELQEAIEGLVEQKRRDLEQADKLENINFTAELLFAQ 8186 (0)
8084 NHGELSAENVMQCVLEMVIAAPDTLSVSLFFMLLLLKQNPDVELQLLQEIDAVVGK (0 expected, bad boundary)
RQLQNGDLQKLRVLETFINECLRFHPV 7719
7718 VDFTMRRSLSDDVIEGYRVPKGTNIILNTGHMHRTEFFLRPTEFCLQNFEKN 7563 (0)
APRRYFQPFGSGPRACVGKHIAMVMMKSILVTLLSQYSVCPHEGLT 7327
7326 LDCLPQTNNLSQQPVEHQEEAQQLSMRFLPRQRGSWQTV* 7207
CYP19A1 Danio rerio (zebrafish)
GenEMBL AF183906
Chiang,E.F.L., Yan,Y.L., Guiguen,Y., Postlethwait,J. and Chung,B.C.
Two Cyp19 (P450 Aromatase) Genes on Duplicated Zebrafish
Chromosomes Are Expressed in Ovary or Brain
Mol. Biol. Evol. 18 (4), 542-550 (2001)
Called CYP19a expressed in ovary
CYP19A2 Fugu rubripes (pufferfish)
No accession number
Scaffold_4200
65% to both brain and ovary forms of CYP19 from Zebrafish
61% to other Fugu CYP19
this is probably the brain form
2918 MKPKETLNITASGPFTPLPLPLMMMMMMLLLMMMMLFLTWNRPQRQHVP (1)
3451 GPLFLAGLGPLLSYCRFMWTGIGTACNFYNNKYGSLVRVWINGEETLILSR (2)
3674 SSAVYHVLRSAHYTARFGSRAGLECIGMEGQGVIFNSDVQLWRRARVYFSK (1)
3960 ALTGPGLQRTVGVCVTSTAKHLDCLVDMTDASGHVDALNLLRAIVVDISNRLFLRVPLN 4136 (1)
EKDLLTKIHNYFETWQAVLIKPDIFFKIGWLFDKHRRAA 4319 (2)
QELQDTMAALLKVKRKLVHEAEKLDDVLDFATELILAQ (0)
EAGEFSADNVRQCVLEMVIAAPDTLSISLFFMLMLLKQHPDVELRIVEELSTvsrt (0)
egeENIDYQRLKVMESFINESMRFHPVVDFTMRKALEDDTIEGIRIRKGTNIILNIGLMHKTE 5039
5040 FFPKPREFSLTNFEQT (0)
VPSRFFQPFGCGPRSCVG 5219
5220 KHIAMVMMKAILATLLSRYTVCPRHGCTLTSIRQTNNLSQQPVEDEHSLAMRFIPRTIQSPS* 5408
CYP19A2 Danio rerio (zebrafish)
GenEMBL AF183908
Chiang,E.F.L., Yan,Y.L., Guiguen,Y., Postlethwait,J. and Chung,B.C.
Two Cyp19 (P450 Aromatase) Genes on Duplicated Zebrafish
Chromosomes Are Expressed in Ovary or Brain
Mol. Biol. Evol. 18 (4), 542-550 (2001)
Called CYP19b expressed in brain
CYP19 Trachemys scripta (red-eared slider turtle)
no accession number
Crews,D.
Temperature-dependent sex determination: the interplay of steroid
hormones
and temperature.
Zool. Sci. 13, 1-13 (1996)
CYP20 human
GenEMBL AC011737.8 chromosome 2 clone RP11-33N4,
AC011737.8 chr 2 (missing exons 12,13)
AC080075.2 (missing exons 1,7,8)
MLDFAIFAVTFLLALVGAVLYLYP (0)
ASRQAAGIPGITPTEEK (2)
DGNLPDIVNSGSLHEFLVNLHERYGPVVSFWFGRRLVVSLGTVDVLKQHINPNKTS (1)
DPFETMLKSLLRYQSGGGSVSENHMRKKLYENGVTDSLKSNFALLLK (0)
LSEELLDKWLSYPETQHVPLSQHMLGFAMKSVTQMVMGSTFEDDQEVIRFQKNHGT (0)
VWSEIGKGFLDGSLDKNMTRKKQYED (1)
ALMQLESVLRNIIKERKGRNFSQHIFIDSLVQGNLNDQQ (0)
ILEDSMIFSLASCIITAK (1)
LCTWAICFLTTSEEVQKKLYEEINQVFGNGPVTPEKIEQLR (2)
YCQHVLCETVRTAKLTPVSAQLQDIEGKIDRFIIPRE (0)
TLVLYALGVVLQDPNTWPSPHK (2) genomic seq stops here the rest is cDNA
FDPDRFDDELVMKTFSSLGFSGTQECPELR (2) intron site based on fish genomic DNA
FAYMVTTVLLSVLVKRLHLLSVEGQVIETKYELVTSSREEAWITVSKRY
CYP20 mouse
GenEMBL AK020848 adult retina cDNA plus ESTs for C-term
MLDFAIFAVTFLLALVGAVLYLYPASRQASGIPGLTPTEEKDGN
LPDIVNSGSLHEFLVNLHERYGPVVSFWFGRRLVVSLGTTDVLKQHFNPNKTSDPFET
MLKSLLGYQSGGGSAGEDHVRRKLYGDAVTASLHSNFPLLLQLSEELLDKWLSYPETQ
HIPLSQHMLGFALKFVTRMVLGSTFEDEQEVIRFQKIHG
TVWSEIGKGFLDGSLDKNTTRKKQYQEALMQLESTLKKIIKERKGGNFRQHT
FIDSLTQGKLNEQQILEDCVVFSLASCIITAR
LCTWTIHFLTTTGEVQKK
LCKEIDQVLGEGPITSEKIEQLSYCQQVLFETVRTAKLTPVSARLQDIEGKVGPFVIPKE 360
TLVLYALGVVLQDPSTWPLPHRFDPDRFADEPVMKVFSSLGFSGTWECPELXFAYMVTAV 540
LVSVLLEKLRLLAVDRQVVEMKYELVTSAREEAWITVSKRH*
CYP20A1 Bos taurus (cow)
See cattle page for details
MLDFAIFAVTFLLALVGAVLYLYPASRQAAGIPGITPTEEKDGN
LPDIVNSGSLHEFLVNLHERYGPVVSFWFGRRLVVSLGTVDVLKQHINPNKTLDPFET
MLKSLLRYQSDSGNVSENHMRKKLYENGVTNCLRSNFALLIKLSEELLDKWLSYPESQ
HVPLCQHMLGFAMKSVTQMVMGSTFEDEQEVIRFQKNHGTVWSEIGKGFLDGSLDKST
TRKKQYEDALMQLESILKKIIKERKGRNFSQHIFIDSLVQGNLNDQQILEDTMIFSLA
SCMITAKLCTWAVCFLTTYEEIQKKLYEEIDQVLGKGPITSEKIEELRYCRQVLCETV
RTAKLTPVSARLQDIEGKIDKFIIPRETLVLYALGVVLQDPGTWSSPYKFDPERFDDE
SVMKTFSLLGFSGTRECPELRFAYMVTAVLLSVLLRRLHLLSVEGQVIETKYELVTSS
KEEAWITVSKRY
CYP20A1 Sus scrofa (miniature pig)
no accession number
Haitao Shang
Submitted to nomenclature committee May 23, 2007
partial sequence
92% to human 20A1, full seq. is not known
Ortholog of human CYP20A1
CYP20A1 Sus scrofa (pig)
DT324969.1, BG894896.1, BI336725.1, BP449309.1
Trace file gnl|ti|861177001
lower case = cow seq.
MLDFAIFAV
TFLLALVGAVLYLYPASRQAAGIPGITPTEEKDGNLPDIVNSGSLHEFLVNLHERYGPVV
SFWFGRRLVVSLGTVDVLKQHINPNKTSDPFETMLKSLLRYQSDSGNMSENHMRKKLYEN
GVTNSLQSNFALLLKLSEELLDKWLAYPESQHVPLCQHMLGFAMKSVTQMVMGSTFEDEQ
EVIRFQKNHGTVWSEIGKGFLDGSLDK
NHMRKKLYENGV
TNCLRSNFALLIKLSEELLDKWLSYPESQ
HVPLCQHMLGFAMKSVTQMVMGSTFEDEQEVIRFQKNHGTVWSEIGKGFLDGSLDKSTTRK
KQYEDALMQLESILKKI ikerkgrnfsqhifidslvqgnlndqqiledtmifsla
scmitaklctwavcflttyeeiqkklyeeidqvlgkgpitsekieelrycrqvlcetv
RTAKLTPVSARLQDIEGKIDKFIIPRETLVLYALGVVLQDPGTWSSPYK
FDPERFDDESVMKTFSLLGFSGPQEC
PELGWRYMVTTVLWSVLLRRRHLLPVEGQVIETNYDLVTSS
XEEAGITVSKRFRPGILLPWLRKLTI*
CYP20 Gallus gallus (chicken)
BU454844 603215388F1 CSEQRBN14 Gallus gallus cDNA clone ChEST201o21 5'.
BU111630 603125978F1 CSEQCHL13 Gallus gallus cDNA clone ChEST95k19 5'
BU356654 603474052F1 CSEQCHN70 Gallus gallus cDNA
MLDFAIFAVTFLLILVGAVLYLYP
ASRQASGIPGLAPTDDK
DGNLPDIIASRSLHEFLVNLHEKYGPLVSFWFGRRLVVSLGSIDLLKQHVNPNRSS
DPFEMMLKSFLRYQSSLNGDTGESHLRRKLYESGVSKSLQSNLALIQK
LSEELLAKWLSLPEAQHIPLCQHMLGFAMKSVTQTAMGSSFEDDQEVIRFRRHHDA
IWSEIGKGFLDGSLDKNATRKKLYED
ALKEMESTLRKVITGTPRQIIQQAFIDTLLQGNLSDQQ
ILEDTMIFSLAGCIITAN
LCTWAVYFLTTSEDVQQNLCKEVDHVLGKGPITHEKIEQLR
YCRQVLCETVRTAKLTPIAAQLQELEGRVDQHTVPKE
TLVLYALGVMLQDSSSWPSPYK
FDPERFSEDSAMTNFSLLGFSGSQECPELR
FAYMVATVLLSILVRKLYLHPVKGQVMETKYELVTSPKEEAWITVSKRS*
CYP20 Xenopus laevis (African clawed frog)
BJ037591.1 NIBB Mochii normalized Xenopus neurula library Xenopus laevis cDNA clone XL040p15 5'
BQ725586 BQ725586.1 AGENCOURT_8103453 NICHD XGC Emb2 Xenopus laevis cDNA clone
95% identical to tropicalis seq. 74% to chicken
BC044111.1 mRNA, complete cds Length = 2417
175 MLDFAIFAITFLLILVGAVLYLYPSSRQACGIPGLAPTEEKDGNLQDIVNSGS 333
334 LHEFLVNLHERFGPVASFWFGRRLVVSLGSLDLLKQHINPNKTSDPFQTMLKSLLGYQSG 513
514 VIGEAAESHVQKKLYENGITKALHSNFSVIIKLSEELLAKWGTYPQSQHVPLCQHMLGFA 693
694 MKSVTQTAMGSSFEDDQEVIHFRRNHDAIWSEIGKGFLDGSIERSPSRKKLYEDALMEME 873
874 TVLKKTIKERKGKNPGRHVFLDSLLQGNLSDKQVLEDSMIFSLAGCVITANLCTWAIYFL 1053
1054 TTSEEVQDKLYKEVNRVIGKGPITMDKLEQLSYCRQILCETVRTASLTPISARLQELEGR 1233
1234 VDQHIIPKETLVLYALGVVLQDNTAWPLAYRFDPDRFDDETAKQSLSLLGLSGSQECPEL 1413
1414 RFAYMVAMVLLCVLVRKLNLLPVKGQVMETKYELVTSPKEEAWITVSKRS 1563
CYP20 Xenopus tropicalis
From JGI blast server http://aluminum.jgi-psf.org/prod/bin/runBlast.pl?db=xenopus1&dump=1
And from Sanger http://www.sanger.ac.uk/Projects/X_tropicalis/blast_server.shtml
AL848968.1 Xenopus tropicalis EST, clone TEgg007o12 5'
AL870093 AL870093.1 Xenopus tropicalis EST, clone TEgg120l22 5'
60% to Fugu CYP20, 70% to human CYP20, 62% to zebrafish, 30% to ciona CYP20 like seq
72% to chicken
MLDFAIFAITFLLILVGAVLYLYP exon 1
SSRQACGIPGLAPTEEK exon 2
DGNLQDIVNSGSLHEFLVNLHERFGPVASFWFGRRLVVSLGSLDLLKQHINPNKTS exon 3
DPFQMMLKSLLGYQSGVIGEAAESHVQKKLFENGIIKALHSNFSVVIK exon 4
LSEDLLAKWLTYPQSQHVPLCQHMLGFAMKSVTQTAMGSSFDDDQEVIHFRRNHDA exon 5
IWSEIGKGFLDGSIERSPNRKKLYED exon 6
ALMEMETVLKKAIKERKVKNPGRHVFVDSLLQGNLSDKQ exon 7
VLEDSMIFSLAGcvitan exon 8
VCTWAIYFLTTSEEVQDKLFKEVTRVIGKGPITMDKLEQLS exon 9
YCRQILCETVRTASLTPISARLQELEGRVDQHIIPKE exon 10
tlvlyalgvvlqdntawplayr exon 11 X. laevis
FDPDRFNDETAKQSLTLLGFSGSQECPELR exon 12
FAYMVAMVLLSVLVRKLHLLPVKGQVMETKYELVTSPKEEAWITVSKRS exon 13
CYP20 Fugu rubripes (pufferfish)
No accession number
Scaffold_486
59% TO CYP20 human
MLDFAIFAVTFVIVLVGAVLYLYP (0)
SSRRASGIPGLNPTDEK (2)
11654 DGNLQDIVGRGSLHEFLVSLHQEFGPVASFWFGSRPVVSLGSLQQLRQHINPNHST 11487 (1)
DSFETMLKSLLGYHSGGGGASTDSIIRKKVYQGAIDTTLKNNFPLVLK (0)
LVDELVGKWKSFPEDQHTPLCAHQLVLAMKTITQLALGESFSEDARVIAFRKNHDV (0)
IWSEIGKGYMDGSLEKSTSRKGHYEK (1)
ALSEMESTLLSVVKERKSQRNKSVFVDSLIQSTLTERQ
IMEDCMVFMLAGCAITAN
VCIWALHFLSTSEEVQDRLYKEFEEVLGSSPVSLEKIPQLR
YCQQVLNETLRTAKLTPIAARLQEVEGKVDQHLIPKE
SLVIYALGVILQDSDTWNAPYR
FDPDRFEEESVKKSFHLLGFSGSQTCPELR
FAYTVATVLLSVLVRQLKLHRLKDTLMEVRSELVSTPRDETWITFNLRN*
CYP20 Tetraodon nigroviridis (freshwater pufferfish)
>FS_CONTIG_2529_2 Length = 13425 from http://fugu.hgmp.mrc.ac.uk/blast/
>FS_CONTIG_2529_1 Length = 1009 from http://fugu.hgmp.mrc.ac.uk/blast/
MLDFAIFAVTFVVILVGAVLYLYP
SSRRASGVPGLNPTDEK
XXXXXXXXXXXDIVARGSVQEFLVSLHQEFGPVASFWFGSRPVVSLGSLQQLQQHANPNRSS
DSFETMLKSLLGYHSGGGGASTENIIRKKVYQGAIDATLKNNFPLVLK
LVDELVGKWTSSPEDQHTPLCAHQLVLAMKSITQLALGESFSQDARVVSFRRNYDA
IWSEVGKGFMDGSLERSTSRKGRYEX
ALSEMEATLLSVVKDRKSQRKTSVLVDTLLQSTLTDRQ
IMEDCMVFTLAGCAITAN
VCIWALHFLSSYEDVQDRLHQELEEVLGSGSVSLEKIPQLR
YCQQVLNETVRTAKLTPVAAGLQEVEGKVDQHLIPKE
TLVIYALGVILQDSHTWDAPCR
FHPDRFEEESVRKSFRLLGFSGSQTCPELR
VAYTVATVLLSAVVRQLRLHRLEDTLVEVRSELVSTPREETWITFSRRN
CYP20 Danio rerio (zebrafish)
Assembled CYP20 from zebrafish ESTs
BQ259821 faa04d08.y1 zebrafish fin day3 regeneration
BM185037 fv16g05.x2 zebrafish adult brain
BG985721 2543 NICHD Zebrafish normalized I
BM070720 fu98e06.y1 zebrafish adult brain cDNA
CA472036 AGENCOURT_10739799 NCI_CGAP_ZKid1 cDNA (kidney?)
BI981894 fu52c12.y1 zebrafish adult brain Danio rerio cDNA
BM083017 fu28a11.y1 Campbell zebrafish ovary cDNA MLDFAIFAVTFVIILIGAVLYLYPSSRRASGVPGLNPTEEKDGNLQDIVNKGSLHEFLVG
LHDEFGSVASFWFGARPVVSLGAVNQLRQHINPNWTTDSFETMLKSLLGYQSGSGVGLTE
SMMRKKVYEGAINKTLENNFPLLLQQVEELVDKWASYPKSQHTPLCAHLLGLAMKAVTQL
AMGSRFRDDAEVIRFRKNHEAIWSQ
IGKGYLDGSLEKSSSRKAHYESALAEMESVLKSVAKQRPGQGSSQSFVNYLLQANLTER
QVMEDGMVFTLAGCVITANLCIWAVHFLSVSEAVQDRLYHELVEVLGDEPVSLEKIPQLR
YCQQVLNETVRTAKLTPVAARLQEVEGKVDQHIIPKETLVIYALGVVLQDADTWSLPYRF
NPDRFAEESVMKSFSLLGFSGSQACPELRFAYTVATVLL
STLVRRLRMHRVDGQVVEARYELVTTPKDDTWITVSKRN*
CYP20 Danio rerio (zebrafish)
>ctg10765 genomic contig CYP20 74% to fugu
9501 MLDFAIFAVTFVIILIGAVLYLYP (0) 9572
SSRRASGVPGLNPTEEK (2?)
DGNLQDIVNKGSLPEFLVGLH
DEFGSVASFWFGARPVVSLGAVNQLRQHINPNWT (1) 10024
12291 TDSFETMLKSLLGYQSGSGVGLTESMMRKKVYEGAINKTLENNFPLLLQ (0) 12439
12929 QVEELVDKWASYPKSQHTPLCAHFL 13003 frameshift
13003 GLAMKAVTQLAMGSRFRDDAEVIRFRKNHEA (0) 13095
15738 IWSEIGKGYLDGSLEKSSSRKAHYES (1?) 15815 AC boundary istead of AG
15897 ALAEMESVLKSVAKQRPGQGSSQSFVNYLLQANLTERQ (0) 16010
16583 VMEDGMVFTLAGCVITAN (1) 16636
17689 LCIWAVHFLSVSEAVQDRLYHELVEVLGDELVSLEKIPQLR (2) 17811
19293 YCQQVLNETVRTAKLTPVAARLQEVEGKVDQHVIPKE (0) 19403
21269 TLVIYALGVVLQDADTWSLPYR (2) 21334
21425 FNPDRFAEESVMKSFSLLGFSGSQACPELR (2) 21514
FAYTVATVLLSTLVRRLRMHRVDGQVVEARYELVTTPKDDTWITVSKRN* from wz10135.3
CYP20 Oryzias latipes (medaka fish)
BJ524824.1 MF01SSB cDNA Oryzias latipes cDNA
BJ003683.1 BJ003683 MF01SSA cDNA Oryzias latipes cDNA
MLDFAIFAVTFVVILVGAVLYLYPSSRRASGVPGLFPTDEKDGNLQDIVDRGSLHEFLV
GLHEQFGPVASFWFGRQPVVSLGSVDPLRQHINPNHTTDSFETMLKSLLGYQAGAGGGAN
ESVMRKKLYESAINNALKNSFPAVLKVAEELVDKWSSVPEDQHIPLCAHLLGLALKTV
TQLALGERFKDDAEVISFRKNHEAIWSEIGKGYMDGSLEKSSSRKRHYE SA
LSDMEATLLAV AKDRKAQRRQTA FVDALLQSGLTERQIMEDCMVFTLAGC
VITANLCIWALHFLSTAEDVQEKLCQEVEDLFGSDPVSLDRIPQLKYCQQVLNE
TVRTAKLTPVAARLXEVEGKVGQHVIPKETLVIYALG
VVLQDADTWSTPYRFDPDRFQDESARKSFCLLGFSGSQTCPELRFAYTVATVLLATLVRRLKLRPLK
About 26 aa missing at end
CYP20 Salmo salar (Atlantic salmon)
GenEMBL CA063128.1 ssalrgb509318 mixed_tissue Salmo salar cDNA.
GenEMBL CB516811.1 ssalrgb509318_rev mixed_tissue Salmo salar cDNA.
GenEMBL CB513409.1 ssalrgb531212_rev mixed_tissue Salmo salar cDNA.
BG935303.1 SL1-0624 Atlantic Salmon liver Salmo salar cDNA clone SL1-0624
MLDFAIFAVTFVIFLVGAVLYLYPSSRSASGIPGLNPTEEKDGNLQDIVNRGSLHEF
LASLHGQFGPVASFWFGGRPVVSLGSVDQLRQHINPNRTTDSFETMLKSLLGYQSGTGGG
ATEAVMRKKLYESAVNNTLEKNFPMLLKLVEELVGKWQSFPKDQHTPLCAHLLGLAMKAVTQ
18 amino acids missing here
RKNHEAIWSEIGKGYLDGSMEKSSIRKEHYESA
LAEMETVLMSVAKDRKGQRSQTAFVDTLLQSNLTERQVME
DSMVFTLAGCVITANLCIWAVHFLST
SEDVQEKLHQELEDVLGSEPVSLDK IPQLRYFQQVLNETVRTAKLTPIAARLQENEGKVD
QHIIPKETLVIYALGVVLQDADTWSCPYKFDPDRFTEDSARKSFSLLGFSGNQACPELRF
AYTVATVVLSTVVRQLKLYQVKGQVVEARSELVSTPKDDTWITVSRRS*
CYP20 Ciona intestinalis
SEQUENCE 186, 187 CYP20 ortholog
29% to fugu CYP20 CYS = HIS
MLEVHVTITAVAVLAVILLLFLIGLLFYIYPSSN (?)
KETTVPGPAVSDIR (2)
DGNRSDIDRAGSLHQYLCNLHAEHGDLASFHFDKQLIISIADSKYFAPRSQVFQLP (1)
DFLYEFWEPMIGKESFVFSQGVDVFKRRDNIDFKVDKKVKEFYAKIF (0)
RLLAIELEEKWRKQPEDQHIPLTQHLKGLATKAVCQMMFGESFADDERVIKLYKAWDK (0)
CWNEVMAASKMEPTKSESDEKK (1)
SIKELRSLMEKAIEERWKKSKHLDGITLLDGLLASSNSSVSPLSSEQLLCDVITMFTMGVQVTNA (1)
VLCWSVYFIAAHDDVQTKLRNKLNEVESLDIMPQSLNDVK (2)
YMQSILKESIRCSGVFSYSGRVEDIDITVGDHLLPKG (0)
TPIIEALMFPMQSDAFESPDM (2)
FNPKNFSEATKSVNPLTYIPFAFGGSRHKPQDS (2)
LVYLLVSITVASLFRNLRISLAGKEVDPQSANYESFVALPSTEEVWVTVGGKLDVETVGENN*
CYP20 Ciona savignyi
sequence for CYP20 ortholog
72% to C. intestinalis seq 186
MLEVHVSITAVAILVLIILLFLIGLLVYIYPVI (2)
KETTVPGPDVSDAK (2)
DGNKSDIERAGSLHAYLCNLHADFKHEGAVSFHHGKQLVISISNPKFFAPRSQVFQLP (1)
SFLYEYWRPLIGEESFLFAKDIDVFRRRDYLDFKVNRNVKEFYAKNF (0)
LAVELETKWEKQPEDQHIPLTQHLKGLATKAVCQMMFGDNYADGTKVLELYRAWDK (0)
CWNEVMAASKMEPTKSESVEKK (1)
SISELRKLMENAIEERWNKSKSLDGITLLDGLLSSQTSDSKDQLTSEQLLCDVITMFTMGVQITTA (1)
VLCWSCYFVASNKEVQRKLKNQLNESKSLDIMPETLMDME (2)
YMQGILKESVRCSGMFSFAGRVEDIDITVQNHLIPKG (0)
TPILEAIMTEMRSEAFSCPDV (2)
FNPKNFTKDNNHLNPLTFIPFAFGGSRHKPEES (2)
LVYLLAAVTVSSLFRNLQVSLAGKEIEPFAANFESFVAMPSTEEIWVTVSKEKEVENVG*
CYP20 Halocynthia roretzi (tunicate, Urochordate)
AV385510
51% to CYP20 in Ciona
KYMKMVLKESLRSGCVFSWAGRQQDIDINVGGNLITKYTPVIEAIGTAMTDEGNFSDPSE
FDPKRFSEDNDEVINPLDYIPFGFGGSRKAPQNSLVFLLASIVASTLIRNLKVCIAGDSS
QNQPDPDFSAFVSRPSKEELWVTVSALGKKKAETVG*
CYP20 Molgula tectiformis (tailless ascidian, tunicate, Urochordate)
CJ366548.1 EST
N-term
MDVQITLATLGVFIVLFVLFLLGFLFYLYPGSNNQSTIPGPNPSDAKDGNVSDLQ
RVGSLQKYLEELHSEYGDIIAFWHQSNQIVSIADLDEFAQTNQVFERPDILFSSWIPLIG
KDSFIFEKDVKNFQRLDML
CYP20 Branchiostoma floridae (amphioxus)
There are 8 CYP20s in amphioxus (see the amphioxus page)
CYP20 Saccoglossus kowalevskii (Acorn worm, hemichordate)
FF488963, FF488605, FF525072, FF503042, FF480950, FF429742
38% to Paracentrotus lividus, 30% to CYP20 human
33% to sponge CYP38A1
MTALVIIVVVLLVVVVAVFYLGTLFGSKQEQMLARIPGMNPTNSED
GNMPDITVAGSLHEFLVKLHTKYGHVATFWYGKTHVISLASAKAFKDTNKLFDRPAVLFNFVKPLIGPD
SIQYANEEDGKRRRHDYDQSFSYDAIKNYYSKFYEATDAVVAKIESLPSDEHINITEHMS
LLVLKALSHATYGDFFKDDEFSITMLRHYETAMAILNQDISKDTEAGQ
RFAQALKTWHDFIRAMIQHRRDNPPNDDDWTFIDVLMAKSSSEEELISDATSYF
IAGYHTTAFMMVWTFYYMCENQEVQEKIYQEIIEVIGKDEQVNYVNLESLKYMRCVFD
ESMRCSVLAPFAARVNMHADMVVQGYTIPKGTPVVHALGVVLMDDEIWSEPERFIPERFL
PXNVSTRHPLSFQPFGFAGKRKCPGYRIAYAEGAVFLATLCRK
FKFQLVEGQTIERKYGFVTIPSNDIWVTVTKRD*
CYP20A2 Saccoglossus kowalevskii (Acorn worm, hemichordate)
FF425228.1
45% to CYP20A1 Saccoglossus
N-term region
FFQFVKPLFGENSIQFANGNYGKQRRKLHDASFTHQAVKSYYPVFQEKTEIFIRKIMAPP
PEEHIPLSEYVSVFVMKSLMSALFGQNVENDEQTVTMLRHYETVMLVLNKKMSANTEKKN
TFHIALNAWHEFIRKIILKRRNEPSDE
CYP20 Strongylocentrotus purpuratus (purple sea urchin)
GenEMBL XM_787803.1, XM_793215.1
AAGJ02125342.1 (middle piece, two exons that were missing)
85% to Paracentrotus lividus
37% to CYP20 Saccoglossus kowalevskii (acorn worm)
35% to sponge CYP38A1
MLDFAIFAVTFIILLVGLLIYIYP (0)
TTPQKTTTVPGLEPSDPV (2)
KGNLDEIGDAGSLHQFLTKLHAEHGDIASFYFTDQLCVSITSPELFKEHQAVFNRP (1)
ALLFKLFEPLITPDSIQYANGGDGRKRRDLTDRCFGFQALQNFIGVFNK (0)
ITEQLVKKISALPPGEHISLHGTGLGLAIKGIMLTSFGDYFQDDKSITSFRKDYDF (0)
VWGEMEARLDGNMPDEGSNRVKTFEE (1)
1967 ARERMYATIQAVIKFRRSNPPSQDKEVFIDVLLKADYPPGRLQADLLTYVIGGFHTSGN (1) 1791
359 LFAWALFFLAEDDKIQEKLYNHVIDIVGKTGEVSMEDIAEMT (2) 234
YMRQVIDETLRVSILAPYAARYQDFDVVLGGHVIPKG (0)
TPVIHALGVSLQSEKYFPNPKT (2)
FNPENFSAANVKKRPRDAYHPFGFAGRRVCPGQQ (2)
FAYKEVAIFLAVFIRAFKVKVVPGQKPEHVHGLVSHLVNKDGGEVWITVEKR*
CYP20 Paracentrotus lividus (common urchin, Deuterostomes)
AM536943, AM531351, AM584793,
AM534834, AM554260
85% to CYP20 Strongylocentrotus purpuratus
32% to human CYP20 (best match in human) first 11 aa identical
37% to sponge CYP38A1
CYP20 has a highly conserved N-terminal
MLDFAIFAVTFVLLLVGLLIYIYPTTPQKSTTVPGLEASDPVKGNLDEIGDAGSMHQFLTKLHED
HGDIASFYFTDQLCVSIASPELFKEHQAVFDRPPLLFKLFEPLITPDSIQYANGSDGRKR
RDLTDRCFGFQALQNFIGIFNKITEQLVEKISALPPGEHVPLYSTGLGLAIKGIMKTSFG
DYFKDDKSINSFRRDYDYVWAEMEARLDGSMPEPES
EREKKFDEARERLYDTIRGVIKVRRSNPPSQDKEVFIDVLLKADYPPGKLLADL
VSYSVGGFHTSGNLFAWALYFLAQDEEIQEKLYKHVIDIVGKTDEVGMEHIAKMTYMR
QVIDETLRVSILAPYAARFQDFDVVLGGHVIPKGTPVVHALGVSLQSEKYFPNVKVFNPE
NFSPANVKKRPRDAFQPFGFAGRRVCPGQQYAYKEVSI
FLAVFLRAFKIKLVPGQKTEHVHGLVSHLVSKDGGDVWFTVEKR*
CYP20 Rhipicephalus appendiculatus (brown ear tick, arthropod, arachnid,
ecdysozoa)
CD784670.1
35% to CYP20 Danio, 39% to CYP20 human
N-term
MLDFAIFAVCFVVFLLALVLYLYPSSAKQTTIPGLEPSDKKEG
NVGDIVQAGGLQNFLISLHKEHGPIASFWIGTKLVVSIGKADLFKTQSHVFDKPAELFVL
YRDVMGAGSIFFANGAEARKRRRLIDEVLTGKSLDKFLGPIEKLCSEVVMHLKDTPDDEH
VPVYQYMYALCMKISTRLLFGEYFFDDMEVLKFSRNFELCIKELEE
CYP20 Ixodes scapularis (blacklegged tick, arthropod, arachnid, ecdysozoa)
EW872941.1
EW945756.1
N and C-term with a gap in the middle
MLEFAIFAVCFVVFLLMLVVYLYPGSSKQT
TIPGIDPSDPKEGNVGDIVQAGGLKNFLLSLHKDHGPITSFWMGTKLVISIGDAELFKTQ
THIFDKPADLFELYRDLVGADSILFANGAEARQRRRRLDDVLTGDRINDFVGPLQKMCAE
VVDRWESLPAEEHVPIYQYMYALCMKTCTRLLFGDYFFDDKHVLSSAGPLSCVSVLQRCC
ACPRPDSLDQSMR
MTWALYFLATH
LKLQDFMQLSNLQKTIKETLRTATVEPWTARCQDVDVEIAGHVIPKKTPVIQALGVVLHE
EDHWKVPHRFDSSRFNEKFPELAYCPFGFAGMRLCPGKDLSVLHMSVFLVTLCHRL
KLRLIQDQVVTPTFALITKTDDEVWLTLERRRP*
CYP20 Amblyomma cajennense (tick)
EC780049.1 EST
N-term
MLDFAIFAVCFVVFLVALVVYLYPSSTKQTTIPGLGPSDAKEGNVGDIVQA
GGLQNFLVSFYKEHGPIASFWIGTKLVVSIGQGELFKTQSHVFDKPADLFVLYREVMGTG
SIFFANGAEARKRRKLIDDVLTGQRLDKFTLPIEKLCSEVVA
CYP20 Helobdella robusta (leech, annelid worm)
JGI gene model fgenesh4_pg.C_scaffold_12000405 [Helro1:168041]
Helro1/scaffold_12:3739808-3743927
28% to CYP20 Saccoglossus kowalevskii,
29% to Strongylocentrotus purpuratus CYP20,
30% to Paracentrotus lividus CYP20
33% to Amphioxus CYP20
MLDYLIFAATVVVFLILAVIYLYPGSKKSIDIPGPEPVDPLEGNYPDIKACGSLHEFLVDLHETYGPICS
FWAGPQLCVSVASPELFSEQANSFNRPVELFKAQTLLWGENSPFVLNGAEGRKKHAFLTGIFNEKYASCS
LKLEHEILKKQTASWLKWPNEQFFPLYEKISGFVVKFLLSSHFGYDAENSKDVNVIKGNLDNVWQEVGHY
MDDTSDADGLRDIRFEQYKKKLKSFISDALILRKTLPTVSDPAKLPIDHVLQLSEEEAVSILLLLFTNAY
MKYCSLFTWMGYFLALEPHVQDQVRKELGSIPIEEINLANCNEFKYFKKVFQETLRCANIVSHTARIQNT
DIVIQNSKIPAN (0)
TPVIQSLGVVSSNPTIFHEPDK (2)
FNPDRHASKNTPCSSFAS
3747683 QYFGFAGKRICPASSIVMSQAALFFAHVLECFSLTLAPDQVVSSHYGLFA 3747832
3747833 KPKEEIWLSV 3747862
ENINVRRVS*
CYP20 Haementeria depressa (leech, annelid worm)
CN807321.1 EST
67% to CYP20 Helobdella robusta
NAYLKYSSLLTWMGYFLALEPDVQHKLRKELGLVSVSDLSSVNINKFKYFQNTLKETLRC
AKIVPYTARIHNTDIVIKKDKIPANTPIIHALGVVSSDPAIFPEPDEFNPERFDSKNKPN
TFACQSFGFAGKRKCPASSTVASHAALFYSHLLDCFELKLAPDQIVSSHHGLNAKPKEEI
WLSLEM
CYP20 Hirudo medicinalis (medical leech, annelid worm)
EY484183.1 EST
VEADKLNMQDMXRLDYLQLVTKEVLRCAGIVPFTARCQNTDSQLESHRIPAG (0)
TPVLHAIGVVSNDSQIFDNPEE ()
FNPERFKNVNPSQFAFHPFGFAGQRTCPGSQVAIAQVATFYAHVLRSFELELAPNQ
LAAPNHGFYVRPKEEVWLTLKARL*
CYP20 Eisenia fetida (earthworm, annelid worm)
EH670454.1, EH670439.1 ESTs
ANKMKYLNQVLKETLRVARIATFAARVHHSDVVLGGYQVPAEMPILHAIGLSLHDEEVFP
HPLKFDPDRFSVENEKHLPPFSFEPFGFSGKRKCPGYNFSYAEATVYLSLLIRSFKIELA
FDQVAESKFGFVTKIKDDLWVIARPM*
CYP20A1 Capitella sp. I (polychaete worm, annelid)
JGI gene model e_gw1.28.81.1 [Capca1:145552]
+ e_gw1.28.94.1 [Capca1:145544]
Capca1/scaffold_28:376236-381170
And Capca1/scaffold_28:374918-376952
upstream region with N-term
45% to Amphioxus CYP20, 41% to Strongylocentrotus purpuratus CYP20
34% to Helobdella robusta CYP20
MIQFLLFAVFFLVVLVVTVIYLYSGSGSRKSTIPGPEPLDDLEGNNAD
IKASGSLHEFLMDIHADYGPMASFLFGPQLVVSICCPDLYKQYLKTFDRPASLFEFLEPLITANSIQFAN
GAEGRMRHSAYMRSFGDSMVKNYYPMLQ KFASDLTRSLQLEAKETHIPLRKYMFAFAIKAMLRGAYGKYF
DSDEEIHKLRAAYDVCWTDLEHRLLGDLPVEGSKRDERFEKNKKIMYDIMQKVLDDRRSNPPENDERCFV
DALIDLDVSEDVKKSDMLTVLIAGFHTTGLLLTWCLYYLAKHEEIQDKLLGEIEAVLGKDGVVKADN VHQ
LPYLRQVLNESLRCSVLAPFAARVQSTDVEIGGHTIPANTPVLMALGVTLCNDEFYPDPEIFDPERFTEE
NMKNRPSLAFEPFGFGKRKCLGYKFSMAEFSVLIPTLIRHLKVELVPGQVVTPMFGLVTTPTDEIWVTVS
ERK*
CYP20A1P Capitella sp. I (polychaete worm, annelid, Lophotrochozoa)
JGI gene model fgenesh1_pg.C_scaffold_1378000002 [Capca1:185650]
Capca1/scaffold_1378:17781-19064
Pseudogene
51% to the N-term and
72% to the C-term of the whole gene in Capitella
& = frameshift
MIPIIYLLVVILVVIVLAALFVQAGNSQIRKSTIPGPKPRNKHEGNLADIRAIGSLHQFLLNLHGNFGPI
ASFHYGAQMVVSISSPDLYKKYLKTFDRPPVLFEFPMISKHSLLLTNGQDAHIRREAYLHTFSDVAIKQQ
YPMLQG
(deletion of 179 aa)
LHQIP&
FLRQVMNESVRCSVLGAVAGRVQAVDVEVGGHQIPAH (0)
TPVVMAIGVTHVNEEFYPAPERFDPGRFTEENVKSRPSLSFNPFGFGKRKCLGYKFSNAEFYVF
ITTLLRCLKVELVPGQVVTPLYRVVTTPADEVWVTLSPRY*
CYP20 Lottia gigantea
jgi|Lotgi1|82740|gw1.84.105.1
37% to CYP20 Danio
EST FC700835.1
MLDFAIFAVTFFMMLVGAVIYLMPSSRKATSVPGLDPTTKEDGNLSDIGRAGSLHEF
LLDLHGRFGDIASFWMGRQLVISIASPELFKQHQNVFDRPVELFKVFEPVIGERSIQYCN
GNDGRARRQTYDKSFSHHELFSYSPKFQEVADNLVKTLENKVKEEHLSLNQYMIAYAFKT
VMNSLYGDAMDDEKDIIAMRGHYDIMWSEMEKRIADPTVPEDGSPRAISLNLALKEMRKI
VQNIIDFRKKNGKKSEDYLLIDRIVEHMKTEDGIFSEILTFL IGGFHTAGNLLTWAVYFL
ATHEEVEKKLLKEIKNVLGDDDISADNYDQLKYTKQIIDETMRCAVVAPWAARYQDFDSE
LGGHKIPKKTPVIHALGVSLQDERQWPQPKIFDPERFNEENIKKRPHLAFSPFGFAGRRV
CPGRKFAYLESAICLVSLFRRFKFHLVEGQVVQPVFGLVTHSEDDIWVTVSKR*
CYP20 Aplysia californica (Califonia sea hare,mollusc, Lophotrochozoa)
EB277115.1, EB214176.1, EB282241.1
MLDFVIFAVTFLTVLLVAIIWLYPSSKKVTTIPGVDPSSK
EDGNFSDITRAGSLHEFLLDLHSQFGDIAGFWWGQTYVVSIASPELFKAYANVFDRPTELFRF
IEPLISMQSLQFTNGSDGRMRRKAYDRPFSHENLKKHFAEFQTIAGIVEQKWSKKSESDH
IPLGEDMALFSVRATLLTLMGDSFQ
(gap)
VGALHTSAYMLTWAMYFLATH
xxxxxxxxxVQVLGQKDVVTDSNYGQLRYLYQVLQETLRCAVVAPWGARVQDFDTELGGHKVPTK
TPVIHALGVVLQNEKLCPLPKRFDPDRFSTENSKDQPTYAFSPFGFAG
KRTCPGYKFANRGSTVLIATLIKKFEVSMWSDQVVKPVFGLVTRPHEEILLKVSRRK*
CYP20 Crassostrea virginica (Oyster, mollusc, Lophotrochozoa)
EH649216.1 N-term
MLDFVIFAVTFVVALLIAVIYLYPGSKRITTIPGPDLTTKED
GNLADLARAGSLHEYLLDLHAQYGDITAFWMGQELVVSICSPDLFKQHSTVFDRPVSLFK
MFEPLIGEKSIQYANKADGRNRRKLYDPVLSSDYLHHYYTAMQEVSDSLVKKWSSLPKEE
HVPVAQYMIAQALKMA
CYP20 Mytilus californianus (mussel, mollusc, Lophotrochozoa)
ES406577.1 EST
EGGARFQDF
DSELGGHKIPKNTPVIHALGVTQQDEAIWPVPNRFDPDRFSEENSKKRQSLAFQPFGFAG
KRICPGYRFAYAEATVCFVTLLRKFKFNMVEGQVITPVHGLVAHPSEEVWITIDKR*
CYP20 Nematostella vectensis (starlet sea anemone, Cnidarian)
XM_001634458
matches genomic seq NZ_ABAV01008690.1 and ABAV01008690.1
40% to human
31% to CYP20 HUMAN
missing N-term
14466 (2) aga NGNLLDVKKAGSLHEYLLLLHKTYGPVASFMWGTINVVSVADPTLWKE
LRSLFDRP (1) 14639
15646 PELFKLFEPLIGKKSIQYANGVHGRKLRQLFDRGFSHQAVKDYYQ (2) SFIE(0) 15792
15931 (2) DCCSDWFFLCPQVAEKVANQWVIKSSDEHIPIVESMLMLAIRAIGKAGM
GKRFSAEKDVQAFMNAYRT (0) 16134
17805 TWEEMEACLNGSLSTKGSKRDQRFQN (1) 17882
18216 DMEVMRKMIRETISARRNQRTSSPEPLFIDSVLDCDFIDEKEVRK 18350
18790 EDDMISFFVGGFHTTGN (1) 18840
19760 MMSWCFYYLAIHPEIQEKVHQELIDVLGNDAIDPQAASELQ (2) 19882
20039 FCRQVLDETLRCAVVAPWGARVQYDHDLQIGEYVVPKG (0) 20152
20626 TPIITPFGVVLQDPELYPEPQM (2) 20691
21349 FDPDRFSPEAMKELPSHAYEP 21411
21412 FGFAGKRKCPGWRFSIAEGLVFLSVFVRRFKIRLVPGQKVEPVYGLVTTPKEELWVAVEKRK* 21600
CYP20 Hydra magnipapillata (hydra, Cnidarian)
BP508840 BP508840 ESTs
ABRM01022129.1 ABRM01019337.1 genomic
36% to CYP20 C-term
7448 aga LGNLGSLTFDGGIHKFLVENHKRLGPMFSFYWGKELAVSLACPILFKEVATLFNRP (1) 7615
9076 VNQFKLFLPLIGKECVQYANEDRGKKLRQINDRHFSHSSIKSYFKEFKQ (0) 9222
YFLLKVGAELCSKWSSSENTRIPLNQNMLSIAFKTIVKTSFG
agt YTKQVIDEVMRIAVLAPYAARYSDYDIIVDGHLIPKK (0)
TPIILALGTVFQDETIFPEPDRFDPDRFSDKQIEERSALAFQPFGF
AGKRKCPGYRLAYAETLTYTFYIIKNFHISLFDKQSVKMHYGFVTKPSEEIWIKVLRRKNI*
CYP20/CYP38A1 Suberite domuncula (sponge)
GenEMBL Y17816 (1789bp)
Mueller,W.E.G., Wiens,M., Batel,R., Steffen,R., Borojevic,R. and
Custodio,M.R.
Establishment of a primary cell culture from a sponge: Primmorphs
from Suberites domuncula
Mar. Ecol. Prog. Ser. In press
Name change to CYP20 is recommended
MLDFVIFAITAVAGLIGILLFFYFSRSTETKPVSSASPTSTIPR
WSAPPADIEKGDLDVMMKKHGSLHQFLLHLHDNGKTPVTSFWWGKTHVVSFCSPQAFK
ESAVFVNRPVELFVGFEPLITPFSIQYANDEDWVQRSKCLYHTLKGDDLKSYFHHFVQ
IAQEEESLWSSYTSDKEVSLTKEVFPMTIKGIARTCFGDIFKDENELSKMAESYHVCW
RTMEEGVPEAGSKRETEFLKHRRVLEDIIRRIIQERKEGEDLQELPFIDSMLQNYDSE
DKIIADAISFMVGGFHTSGYMFTWMLWYLSSHPESQDRLRTEIERETGGERGDRLKEY
SLRADTFLRQVQDETIRLSTLAPWAARYSDKKVTVCGYTIPAKTPMIHALGVGLKNKT
VWENTDSWDPDRFSPNGRRGNDFCPFGVHSRRKCPGYLFSYFEVGVFASILLSRFEIV
PVEGQTVIQVHGLVTEPKDDIKIYIRSRKED
CYP20 Schmidtea mediterranea (freshwater planarian)
NZ_AAWT01057969
34% to Paracentrotus lividus
33% to Strongylocentrotus purpuratus
32% to Saccoglossus kowalevskii
25% to human CYP20
from SmedGD database
Missing first exon
23008(2)aga KPKQIVIIPGLQKSDPK(2)
22918FGNLLEIKNSGSLHQFLSDLHLKFGAVATFYWNDQKIVSVCSNDLFKSVQDLFDRPSLI
FGNFHEFIGENCLQYANGNEGQNRRKQYDKAFQHDMIIR(2)22625
18726 YISTIENVGKSNLEEWHGSKVTVDSIEHWAYIMAVKSILKACIIPEEQSPEYCDTIITLYKS
VFIGLEKKFVECKVLSDEEEIQFQKDKNQFYSIIREIINKRLDFLENSDLENEKLLLIDY
IILYHRELNRNLIENEVLIYLIGGFHTT(1) 18274
13764(1)GNALSWCIYYLAKHPKCQEKLFTEISKRQIQLQNTSECYKLILD
LKYLKACIEETLRLSQLAPYAARVSDEDRYLSNYEVPANTPIILALGVSLKDEKI
FNNPDQFNPERFVDSNFPSFAFVPFGFAGKRKCPGER(2)13357
13304 FSYLELSIWLIQLVGKYRFTLTNPHQTVNKVYGLVTRMSENVSVQFGERSDVL*13143
21A Subfamily
CYP21A1P human
GenEMBL M13935 (3206bp)
White,P.C., New,M.I. and Dupont,B.
Structure of human steroid 21-hydroxylase genes.
Proc. Natl. Acad. Sci. U.S.A. 83, 5111-5115 (1986)
97% to 21A2 NT_033167.1|Hs6_33343
329415 MLLLGLLLLLPLLAGARLLWNWWKLRSLHLLPLAPGFLHLLQPDLPIYLLGLTQKFGPIYRLHLGLQ 329215
329117 DVVVLNSKRTIEEAMVKKWADFAGRPEPLTCK 329022
LVSKNYPDLSL
328702 XXWSLLWKAHKKLTRSALLLGIRDSMEPVVEQLTQEFCE 328592
RMRAQPGTPVAIEEEFSLLTCSINCYLTFGDKIK 328383
328294 EDNLMPAYYKCIQEVLKTWSHWSIQIVDVIPFLR 328193
328091 FFPNPGLRRLKQAIEKRDHNEEKQLRQHK
327835 ESLVAGQWRDMMDYMLQGVAQPSMEEGSGQLLEGHLHMAAVDLLIGGTETTANTLSWAVV
327654 FLLHHPE 327634
IQQRL*EELDHELGPGASSSRVPYKDRARLPLLNATIAEVLRLWPVV
327292 PLALPHRTTRPS 327257
SISGYDIPEGTVIIPNLQGAHLDETVWERPHEFWP 327069
326971 DRFLEPGKNSRALAFGCGARVCLGEPLARLELFVVLTRLLQAFTLLPSGDALPSLQPLPH
326791 CSVILKMQPFQVRLQPRGMGAHSPGQNQ 326708
CYP21A1P human with congenital adrenal hyperplasia
GenEMBL M26857 (4034bp) X05445
Rodrigues,N.R., Dunham,I., Yu,C.Y., Carroll,M.C.,
Porter,R.R. and Campbell,R.D.
Molecular characterization of the HLA-linked steroid
21-hydroxylase B gene from an individual with congenital
adrenal hyperplasia.
EMBO J. 6, 1653-1661 (1987)
CYP21A1P human
GenEMBL S60612 (426bp)
Collier,S., Tassabehji,M. and Strachan,T.
A de novo pathological point mutation at the 21-hydroxylase locus:
implications for gene conversion in the human genome.
Nature Genetics 3, 260-265 (1993)
CYP21A2 human
GenEMBL M21544 M21545 M21547 to M21550 M23224 M23225
M31022 M31023 (ten segments)
Globerman,H., Amor-Gueret,M., Parker,K.L., New,M.I. and White,P.C.
Nonsense mutation causing steroid 21-hydroxylase deficiency
J. Clin. Invest. 82, 139-144 (1988)
CYP21A2 human
GenEMBL M12792 M23280 (5141bp)
Higashi,Y., Tanae,A., Inoue,H., Hiromasa,T., and
Fujii-Kuriyama,Y.
Aberrant splicing and missense mutations cause steroid 21-
hydroxylase [P-450(C21)] deficiency in humans: possible gene
conversion products.
Proc. Natl. Acad.Sci USA 85, 7486-7490 (1988)
CYP21A2 human
GenEMBL X54940
Partanen,J. and Campbell,R.D.
Rapid characterisation of mutant P450c21 Genes by PCR
unpublished (1993)
CYP21A2 human
GenEMBL X58898 to X58908 PIR S26485 (97 amino acids)
PIR S26484 (371 amino acids) PIR S29670 (495 amino acids)
PIR S29671 (372 amino acids) PIR S26584 (371 amino acids)
PIR S29673 (372 amino acids)
Helmberg,A., Tabarelli,M., Dobler,G. and Kofler,R.
Identification of molecular defects causing congenital adrenal
hyperplasia by cloning of PCR-amplified 21-hydroxylase genes
unpublished (1992)
CYP21A2 human with congenital adrenal hyperplasia
GenEMBL M28548 (4044bp) X05449
Rodrigues,N.R., Dunham,I., Yu,C.Y., Carroll,M.C.,
Porter,R.R. and Campbell,R.D.
Molecular characterization of the HLA-linked steroid
21-hydroxylase B gene from an individual with congenital
adrenal hyperplasia.
EMBO J. 6, 1653-1661 (1987)
Note: mutant gene, 2 amino acid differences
CYP21A2 human with congenital adrenal hyperplasia
GenEMBL M26856 X05448 (4042bp)
Rodrigues,N.R., Dunham,I., Yu,C.Y., Carroll,M.C.,
Porter,R.R. and Campbell,R.D.
Molecular characterization of the HLA-linked steroid
21-hydroxylase B gene from an individual with congenital
adrenal hyperplasia.
EMBO J. 6, 1653-1661 (1987)
Note: normal gene
CYP21A1 Bos taurus (cattle)
GenEMBL M11267, M13545
Chung BC, Matteson KJ, Miller WL. (1986) Structure of a bovine gene
for P-450c21 (steroid 21-hydroxylase) defines a novel cytochrome P-450
gene family. Proc Natl Acad Sci U S A. 83, 4243-4247.
First cattle CYP21A1 gene
CYP21A1 Bos taurus (cow)
See cattle page for details
MVLAGLLLLLTLLSGAHLLWGRWKLRNLHLP
234 PLVPGFLHLLQPNLPIHLLSLTQKLGPVYRLRLGLQEVVVLNSKRTIEEAMIRKWVDFAG 413
414 RPQIPSYKLVSQRCQDISLGDYSLLWKAHKKLTRSALLLGTRSSMEPWVD 563
564 QLTQEFCERMRVQAGAPVTIQKEFSLLTCSIICYLTFGNKEDTLVHAFHDCVQDLMKT 737
738 WDHWSIQILDMVPFLRFFPNPGLWRLKQAIENRDHMVEKQLTRHKESMVAGQWRD 902
903 MTDYMLQGVGRQRVEEGPGQLLEGHVHMSVVDLFIGGTETTASTLSWAVAFLLHHPEIQ 1079
1080 RRLQEELDRELGPGASCSRVTYKDRARLPLLNATIAEVLRLRPVVPLALPHRTTRPSSIF 1259
1260 GYDIPEGMVVIPNLQGAHLDETVWEQPHEFRPDRFLEPGANPSALAFGCGARVC 1421
1422 LGESLARLELFVVLLRLLQAFTLLPPPVGALPSLQPDPYCGVNLKVQPFQVRLQ 1583
PRGVEAGAWESASAQ*
CYP21A1 pig
S53049 M83939
MVLVWLLLLLTLLAGARLLWGQWKLRNLHLPPLVPGFLHLLQPNLPIYLLGLTQRLGPIY
RLRLGLQDVVVLNSKRTIEEALVRKWVDFAGRPQIPSYKLASQHCPDISLGDYSLFWKAH
KKLTRSALLLGVRSSMEPRVEQLTQEFCERMRAQAGTPVTIQKEFSVLTCSIICCLTFGD
KEDTLVHALHDCVQDLMKTWEHWSIQILDMVPFLRFFPSPGLRRLKQAIENRDHLVEKQL
RRHKESMVAGQWRDMLDYMLQEAGRQRVEEGQGQLLEGHVHMSVVDLFIGGTETTANTLS
WAVVYLLHHPEIQWRLQEELDRELGPGAAGSRVPYKDRARLPLLNATIAEVLRLRPVVPL
ALPHRATRPSSIFGYDIPEGTVVIPNLQGAHLDETVWEQPHEFRPDRFLAPGANPSALAF
GCGARVCLGEPLARLELFVVLVQLLQAFTLLPPEGALPSLQPHPHSGINLKVQPFQVRLQ
PRGGRGEGPGPR
CYP21 rat
GenEMBL U56853(1964bp)
Zhou,M.Y., Vila,M.C., Gomez-Sanchez,E.P. and Gomez-Sanchez,C.E.
Cloning of two alternatively spliced 21-hydroxylase cDNAs in the
rat adrenal.
unpublished (1996)
MLLPGLLLLLLLLLLAGTRWLWGQWKLWKLRLPPLAPGFLHFLQPNLPVYLFGLAQKLGP
IYRIRLGLQDVVVLNSNKTIEEALIQKWVDFAGRPQILDGKMNFDLSMGDYSLTWKAHKK
LSRSALVLGMRDSMEPLVEQLTQEFCERMRAQAGASVAIHKEFSLLTCSIISCLTFGDKQ
DSTLLNATHSCVRDLLKAWNHWSVQILDIIPFLRFFPNPGLWKLKQFQESRDHIVMQELK
RHKDSLVAGQWKDMIDYMLQGVEKQRDARDPGQLHERHVHMSVVDLFVGGTETTAATLSW
AVAFLLHHPEIQKRLQEELDLKLAPSSQLLYKNRMQLPLLMATIAEVLRLRPVVPMALPH
RATKASSISGYDIPKDTIIIPNIQGANLDEMVWELPSKFWPDRFLESGKSPRIPTFGCGA
RVCLGEPLARLEFFVVLARLLQTFTLLPPPDGTLPSLQPLPYTGINLLIPPFQVRLQPRN
LAPQDQGQK
CYP21 rat
GenEMBL U56854
alternatively spliced form
Cyp21 mouse
genEMBL AF049850 Mm.18845
MLLPGLLLLLLLLAGTRWLWGQWKLRKLHLPPLAPGFLHFLQPN
LPIYLLGLTQKLGPIYRIRLGMQ DVVVLNSNRTIEEALIQKWVDFAGRPHMLN GKMDL
DLSLGDYSLMWKAHKKLSRSALMLGMRDSMEPLIEQLTQEFCERMRAQAGTPVAIHKE
FSFLTCSIISCLTFGDKDSTLVQTLHDCVQDLLQAWNHWSIQILTIIPLLRFLPNPGL
QKLKQIQESRDHIVKQQLKRHKDSLVAGQWKDMIDYMLQGVEKQRDGKDEERLHEGHV
HMSVVDLFIGGTETTATTLSWAVAFLLHHPEIQKRLQEELDLKLGPGSQLLYRNRMQL
PLLMATIAEVLRLRPVVPLALPHRATRASSISGYDIPKDMVIIPNIQGANLDEMVWEL
PSKFWPDRFLEPGKNPRTPSFGCGARVCLGEPLARLELFVVLARLLQAFTLLPPPDGT
LPSLQPQPYAGINLPIPPFQVRLQPRNLAPQDQGERP
Cyp21a2-ps mouse
GenEMBL AF030001
MLLPGLLLLLLLLAGTRWLWGQWKFRKLHLLPLAPGFLHFLQPNLPIYLLGLTQKLGPIYRIRLGLQ
Gap 30 aa missing
GKMDLDLSLGDYSLMWKAHKKLSRSALMLGMLDSMEPLIEQLTQEFCE
RMRAQAGTPVAIHKEFSFLTCSIISCLTFGDK
DSTLVQTLHDCVQDLLQAWNHWSIQILTIIPLLR
FFPNPGLQKLKQIQESRDHIIKQQLKQHK
DSLVAGQWKDMIDYMLQEVEKQRDGKDKEQLHEGHVHMSVVDLFIGGTETTATTLSWAVAFLLHHPE
IQK*LQEELDLKLGPGSQLLYRNRMQLPLLMATIAEVLHLRPVVPLALPHRATKAS
SISGYNIPKDMVIIPNIQGANLDEMVWELPSKFWP
DRFLEPGKNPRTPSFGCGARVCLGEPLARLELFVVLARLLQAFTLLPPPDGTLPSLQPQL
YAGINLLIPPFQVRLQPRNLAAQELGEKTLTG*
CYP21 pig
GenEMBL S53049 M83939 (4792bp) Swiss Q02390 (492 amino acids)
PIR S28169 (492 amino acids)
Burghelle-Mayeur,C., Geffrotin,C. and Vaiman,M.
Sequences of the swine 21-hydroxylase gene (Cyp21) and
a portion of the opposite-strand overlapping gene of unkown
function previously described in human.
Biochim. Biophys. Acta 1171, 153-161 (1992)
CYP21 sheep
PIR A43349 (497 amino acids) B43349 (479 amino acids)
Crawford, R.J., Hammond, V.E., Connell, J.M. and Coghlan, J.P.
The structure and activity of two cytochrome P450c21 proteins
encoded in the ovine adrenal cortex.
J. Biol. Chem. 267, 16212-16218 (1992)
B43349 is truncated early after alternate splicing
CYP21 Xenopus tropicalis
See Xenopus pages for seq
CYP21A1 Danio rerio (zebrafish)
See xebrafish pages for seq
CYP21 Fugu rubripes (pufferfish)
No accession number
Scaffold_15
44% to CYP21 human over 454 amino acids
this is the first fish CYP21 sequence found
MCSFLFCSSFSAPPAVKRNLPLSLWQLPLRPSSPPIPGPPCRFLIGNMTE
28230 LMHDHLPIHLTNLAKRYGNIYRLKCGNTT 28144 (1)
28058 AMIVLNSSDIIREALVKKWSDFAGRAVSYT 27969 (1)
27888 ADIVSGGGRNISLGDYTEEWKALRRLVHGALQRCCKHSLHNVIERQALQLRK 27754 (0)
VLVDYRGGAVDLSEDFTVAASNVIITLVFGKE (0)
YDKSSSELQQLHRCLNEIVALWGSTWISALDTFPLLR (0)
KFPNPVFSRLLREVSRRDEIIRKHLNQFK (0)
CVLCCAQSEGHRRTDVITGSLLEG (0)
26896 VLTDMHVHMATVDLLIGGSETTAAWLNWTVAFLLHRPE (0)
FQTKVYEELCTVLEGRYPKYSDRQRLPILCSLIHEVLRLRPVAPLAVPHKAIRDS (2)
SIAGYFIPRNTIIIPNLFGAHHDPEVWSDPYSFKP (1)
26238 ERFLEGGGGSTRALIPFGGGARLCLGETVAKMELFLFTAYLLRDFCFVLPDSEAPLPDLR 26059
26058 GVASVVLKIKSFTVIARPRTGP* 25990
CYP21 Tetraodon nigroviridis (freshwater pufferfish)
GenEMBL AL281449.1 C0BG094DE12LP1 G Tetraodon nigroviridis
genomic clone 094J24 T7.Length = 895
AL233853.1 C0BG007BE04XD1 G Tetraodon nigroviridis
genomic clone 007I08 T7.Length = 1079
86% to Fugu CYP21
MGCIFFFFYLPFSAPPAVKRSLLQSLCGLLHRPSSPSIPGPPCRFLIGNMTE
LMQDHLPIHLTDLAKRYGNIYRLKCGNTS
AMVVLSSGDVIREALVKKWSDFAGRSVSYT
ADIVSGGGRTISLGDYTEEWKAHRRLVHSAL (frameshift) ERCXKQSLHDVIERQALQLRK
Missing exon 4 and part of exon 5
GSAWISALDTFPLLR
KFPNPVFSRLLREVTRRDEIIRKHLNQYK
CVLCCVQSQDNKSTDVITGSLLEG
VLTDVHVHMATVDLLIGGTETTAAWLNWTVAFLLHRPE
IQTKVYEELCTVLEGRYPKYSDRHRLPVLCSLVHEVLRLRPVAPLAVPHKAVRDS
SIAGYFIPKNTIIIPNLFGAHHDPXVWPDPYSFXX
Missing exon 11
22A Subfamily
CYP22A1 Caenorhabditis elegans (nematode worm)
GenEMBL U39648 (28581 bp) AF407572, NM_171699, NM_171698
cosmid T13C5.1
Jia,K., Albert,P.S. and Riddle,D.L.
DAF-9, a cytochrome P450 regulating C. elegans larval development
and adult longevity
Development 129 (1), 221-231 (2002)
daf-9 mutant This gene may be in the pathway to
synthesize a ligand for DAF-12, a nuclear receptor.
Daf-9 has a role in larval development.
DAF comes from abnormal DAuer Formation
23A Subfamily
CYP23A1 Caenorhabditis elegans (nematode worm)
GenEMBL U39472 (42282 bp)
see CYP14A1 for reference
cosmid B0304.3
24A Subfamily
CYP24 rat
GenEMBL L04608 to L04619, S52625 to S52636
Ohyama,Y., Noshiro,M., Eggertsen,G., Gotoh,O., Kato,Y.,
Bjorkhem,I. and Okuda,K.
Structural characterization of the gene encoding rat
25-hydroxyvitamin D3 24-hydroxylase.
Biochemistry 32, 76-82 (1993)
CYP24 rat
GenEMBL Z28351 (1419bp)
Hahn,C.N., Kerry,D.M., Omdahl,J.L. and May,B.K.
Identification of a vitamin D responsive element in the promoter of
the
gene for the rat 25-hydroxyvitamin D3 24-hydroxylase
Nuc. Acids Res. 22, 2410-2416 (1994)
CYP24 rat
GenEMBL X59506 (3209bp)
Ohyama,Y., Noshiro,M. and Okuda,K.
Cloning and expression of cDNA encoding 25-hydroxyvitamin D3 24-
hydroxylase.
FEBS Lett. 278, 195-198 (1991)
CYP24 rat
GenEMBL D17792 (?bp)
Ohyama, Y., Ozono,K., Uchida,M., Shinki,T., Kato,S., Suda,T.,
Yamamoto,O.,
Noshiro,M. and Kato,Y.
Identification of a vitamin D-responsive element in the 5'-flanking
region of the rat
25-hydroxyvitamin D3 24-hydroxylase gene.
J. Biol. Chem. 269, 10545-10550 (1994)
CYP24 rat
GenEMBL U03112 (?bp)
Zierold,C., Darwish,H.M. and DeLuca,H.F.
Identification of a vitamin D-response element in the rat calcidiol
(25-hydroxyvitamin D3) 24-hydroxylase gene.
Proc. Natl. Acad.Sci. USA 91, 900-902 (1994)
CYP24 human
GenEMBL S67623 (776bp)
Labuda,M., Lemieux,N., Tihy,F., Prinster,C. and Glorieux,F.H.
Human 25-hydroxyvitamin D 24-hydroxylase cytochrome P450
subunit maps to a different chromosomal location than that of
pseudovitamin D-deficient rickets.
J. Bone Miner. Res. 8, 1397-1406 (1993)
CYP24 human
GenEMBL L13286 (3254bp)
Chen,K.-S., Prahl,J.M. and DeLuca,H.F.
Isolation and expression of 1,25-dihydroxyvitamin D3
24-hydroxylase cDNA.
Proc. Natl. Acad. Sci. USA 90, 4543-4547 (1993)
CYP24 human
NM_000782
MSSPISKSRSLAAFLQQLRSPRQPPRLV