Aspergillus nidulans P450s

sequences submitted by Ralph Dean, collected from the automatically annotated genome.

Some sequences have been modified, but most are as they were collected. There are

several fusions that are possibly artifacts of the annotation process. More work will

be needed to check each gene model and make corrections.

Trees of the fungal P450s are available under the Lower Eukaryotes button on the

main page. For an overview tree see the Magnaporthe grisea tree, then look

at the subtrees for the fungal P450 clans.

List of P450s in name order

http://drnelson.utmem.edu/Aspergillus.CYP.list.htm

List of P450s by contig order (shows gene clusters)

http://drnelson.utmem.edu/Aspergillus.contig.order.htm

Aspergillus has 111 P450 sequences (some are long).

There are 42 NEW CYP FAMILIES even after naming all

P450s from Neurospora crassa, Magnaporthe grisea and Fusarium graminearum.

Fungi have reached CYP687 and will go to 4 digit names after CYP699.

The next number after CYP699 will be CYP7001A1

modified March 18, 2004

Last modified 7/20/2007 revised many of the sequences.

D. Nelson

PREVIOUSLY named seqs in Aspergillus nidulans 53A3, 59A1, 60A2, 60B1, 62A1, 504A1, 504B1

>CYP669A1P Aspergillus nidulans revised 7/18/07

AACD01000002.1 Aspergillus nidulans FGSC A4 AN0008.2 poor match 53 clan

may be a pseudogene of the CYP670 family

34% to 670A1, 29% to 628A1, 37% to AL115607.1 Botrytis cinerea partial seq

since this is a probable pseudogene and it matches 62% to CYP669A2P

after MDGPSN, the N-terminal may not be real but it matches

pretty well to an mRNA called an oxidoreductase.

It may be best to delete the first four lines up to DSPG

WKGHPFTPVVNIVPAFISSLLAFSLSTSQFAKTYRPFKNAPSSLVNHT

VKFAGSSAVHGSHRVSILSSA

SSGSVSGSAPNLLVNAV*TMPGLSA

AAKTDGFSAAIHAMNLVSANLDVMYPDSPG (2)

MDGPSNRNIRSDWYDLLHPQVSSIFTRDRILHQKRRQLWTKALSST(1)

SIQLYTRRILSKIYALES

14773 LIATAADEGKPVLINEVMYWFAFDSVGDFAFSEDFGMLRNEGWHDVIWMFRSALALLGPF 14594

14593 SPAIWMPRLAFSYIPGLWRVKWWFNMLEFCDRCMEQRMK (0)

14416 KTPKESNIASWFLKDHKVHRQNAWLSGDTATLVVAGR

DTNAPTLVHLFYFLARYPEHTEKIW*

ELVSVNAEDPVALAKLPHLNGVINESMRLLPAVL 14054

14053 TFGSRTTQPEGLTVEGTFIPGGVKISAPRYTIGR (1)13952

13871 LKSAFAETHEFIPEWWYSRPDLVKDRRAFAPFGV (1)

GSTSCVGK 13692

13691 SLALAQIRLVTAALVSKYKFQFTPGMGSGEAVEGEMREQLTAQPGHCYLNFIRHVGQE* 13515

>CYP684A1 AN0222.2 AACD01000005

AACD01000005.1 revised

MQPGLSFLVGNWPSVVVLLGFAYFLTLAYGVRKNP

LSSLPGPQLTKWTDLLLKFYTVTGQRPRYVHALHQKYGPVVRISPSIVDI

SDVSASRDIHRIASPFLKAPFYKMLVRKDGESLFSTTDPEYHRRHRRLLS

SPLSDTNLRTVEPLVKARIRLAISRIREEALSPRGVADIYKWFFFMATDI

IGELSFGDSFRMLEIGKKNQYISDLETVAKIGGIRANFPWIISIGQILPL

SIFREVVVSTDRILEYANQSVERYKRHLAMNPNQPKPTLFTKLYDASLHK

EGDGECLSDREIRNDAQSFIVAGSDTTANTLTYLVWSVLKDRSIQEKLVE

ELDVL (0)

EELSDVHLRELQYMNQVINEALRLYPAVPSGLP

RVVPDKGSTLAGHWLPGGATVTTQLYSLHRDEEVFEEPER (2)

FDPSRWENPTKAMKDAYMPFGAGSRN (1)

CIGLHLAKMELRLATAYFFRSFPRARISAREDMNDGDMEMMLYFLLSPKGKRCLVEVN*

>CYP680A1 AN0338.2 AACD01000006

53 clan

AACD01000006.1 Aspergillus nidulans FGSC A4 revised

MDLKESSLRFLASDIRSVIWRLATLISVVALLRTAWVVIYRIYFHPLAKV

PGPLLARITWLYSFWYNIVTKRFYLRVQELHEQYGPVIRITPDEIHLSDP

ENLEKIYFVGSRYPKDAAFYGAFGADRSVFTAPSPDVHRVKRAALNPFFS

RKKVLELEDIVQDKAEKLVGRMKKAFNSTGAIDLHHGFRAISVDVITDYA

FGDSYNFLDRDDFGIDFFNGFRDTGPALWTFQQFPVIQRVAKKMPFWLAE

RLSAPLALRRSQHMSSRAQVLKVKAAVERDEKVSRTTIFHELLRPDAAEG

YAVPNVDELSSEAQNILGAASDTTGNTLTIAAYNVVRNPDIYARLSAELK

EAFPNPEGRLEFVALEKLPYL (0)

TAVIKEGLR (2)

LSFGVPGRLPRVVPPPGAEFNGYHVPPG (0)

TVVSMSSWIIHHNENIYANADRFNPDRWLDPSRAKVLERYLFSFGKGTRGCIGMP (2) 140353

LAYCELYVTLGRMFREFDNLKTPAKSSEELIVDDGFSAYHPEKNNKFVFSLS* 140166

>CYP578C1 AN0459.2 AACD01000007

41% to 578A1 AACD01000007.1 revised 7/18/07

MEVPVFAWIVPIMLGLTLLYEFVIYPAFLSPLSKLPNAHWTAPISPAWILWKRFRGQNNRT

IHAAHERLGSIVRLAPSEISINCVDGGIKSVYTGGFDKHEWYPRVFGSFG (2)

TVSMFT

MTGSKAHSTRKRMLSNIYSKSYLQSSRHLQLISKTIIFDRLLPILQAAAS

SKTDTDVHDLNQGLTMDFVSAYLFGLANGTNFLQNAEYRREMLRLYQCRK

PYEFYHQEVPNILALLKKIGIRLIPKWCDDANRILDSWGLELCDKAEASV

ASSDLSVEPVVYKHLKQAISKQAPLKIDDQKLYADYLEQQRLDIACEMYD

HLTAGHETSAVVLTYLMWELSQHIEVQKDLHKELLTLQPRIAPRCLSGHA

ELPSPKSIDSLPFLEAILTETLRLHASIPGIQPRVTP

TPTCTLVGYDNIPPNTRVNAQAYSLHRNPEVFPDPETWEPKRWL

KDCNKPSELEERKRWFWAFGSGGRMCVGSNLALQ (1)

KMKLVIAAIYTNFRTTIVNDDGIEAIDAYTVKPKGEKLILRFEYI*

>CYP5076A1 = old CYP670B1 AN0606.2 AACD01000007

poor match 32% to 574A1 53 clan

MALSLNAAVVLAGVISHIAYFKQGEHHLYGFFYLKLLLTAMSTATVMLSY

VQGAPWRVALSTVLKLLSAYLFGIYTSLLVYRLLLHPLNRFPGPFPARIS

TVWTSTQLKSNNMHLTLLHYHRKYGPFVRIGSSDLSIAHPDALGPIYGTH

SRCIKGANYELSAPATALQLMRDPEEHHARRRVWSGAFSDRLLRGYEVRI

RKYREKLLDRLSEMSSRKEPVDVTKWFNLYSFDVMGDLSFGRGFEALERG

EEHWAMRLLMATQNFVGLNLPAWAFVLMIRIPGASMDFWRFLEFCGERLL

DRFKNDPEIPDISSSLFVPLKDRNVEDLTIEEKNLLYGDSRLIVIAGSTS

DTTAGTLSAIFYELVQHPEHITKLRDELEPHHLGNDNPKKTEFLHSKIAQ

LDHLNGVINEALRLYPAVPSSLQRKTPPEGVVVDGTYIPGDMHVVCPLYT

IGRSEIAYDHPEDFIPERWYSKPELVRHKGAFAPFSLGHFNCIGRPLALM

NLRVTLAQLIMEFDVKFAPGEDGKQFLADAKDNFVMYFGKLELAFTRRER

QKE*

>CYP578B1 AN1087.2 AACD01000016

42% to 578A1 53 clan

AACD01000016.1 revised

MFTHTAIFTLSFLLTVSFLLFIYLIHPLFLSRLSKLNIPNAHFTSPLSSY

WINSIRHAGLETVTIQALHAKHGPVVRLSPNELSVNSLHGLRVIYTGAFE

KHALYRDLFLNFHTENLVGMLGNKEHARQKRMLSRVYSKSYLQESEDMRV

ISAVILSTRLLPILQRIAENGETVNVLPLFQAVGMDFTSAYLLGVQNGTS

FLFDLPGWQRWLEEYEKFKHLSLNERADGFIERWCLDLCRQTQTESSSSD

AISTKPVVYNALRHGLEKSPDSRPSDLAIASELLDHLIAGHETSGITFTY

MMWELSHCPNLQDELRAELLLLQPSLKYPFPSGGGNGSFPHPSSVDNLPL

LDAIVRETLRLHAPASSPLPRVTPDTPGGTSIDGFDGIPGGITVSSSAYT

LHRIEEVYPQPTEWLPQRWLDPGSGKKHDMRRLWWPFGSGGRMCLGSNFALQ (1)

EIKLVTAAVYTNYTTAVVDDEGIEQDLADFISLPKGRKLVLRFDPINRA*

>CYP668A1 AN1300.2 AACD01000017

poor match 53 clan similar to 548A3

MSNTVYLITGANRGLGLGLTKSLLIRPSTTIIASVRNNEAKDALISELAT

IIPGQNSLLRIIHLSFPSDNWSSAPTSPSEILTALTNTVPEIRHIDVLLA

NAGFATPMTPALSTSVSDLRASFEVNTIAPLLVFQAFWPLLRNAQNGTPK

AIFMSSSVGSIGAQEPFPGGAYGPSRAAGNWLTRALHLQHEADGLVAVAL

HPGWVKTRAGDFVAKEWGFDAGPPETVENSVKGILKTSLLGTGIWTINHN

ERYYPQPFSFRPERWIPEEFGEEAVQLATAAFTTFSIRPWNCVGKALAMI

EMTLAMAAVISHFDFRRADGAAGGLGEGKGSFDGQFQTFWAFTGFKDGPM

IQFRPRKY*

>AN1387.1

probably not a P450 no motifs match

MNSKQYLSYGSADNTHPTDIFALAVTEKQILSASGSNALQVHSTTNPDFP

LVQTLEAHKAGCHHVVTDAKGSRAVSVGFGGEVVIWESHEGTWSKTKDVV

LADIWAVALSADGQYLAGTTQDGHVKVWDMNANEEEIRDHETKGSFGTCI

DLSPDGRFIASGHENGSVYIFSTETGRMPFSLSGLVKPVRSVAFSPGGKF

LAAAGDSRVIVLYDTTSGEQVASLTGHAAWILSLSWSNTGEYLLSGSFDG

KVKVWSIDTRNCVATHSETERAIWSVIWLPKIGKSEGFATAGANRSISFY

REATGG*

>CYP504B1 AN1397.2 AACD01000019

63% to 504B2 100% to 504B1

MAIAEALLFVNNRAVEHPLQFLTAVAFAVPLLYVVINEFIRASARIPGFK

GPRGLPLIGNLAQIRKDAAEQYRIWSKIYGPVYQIQLGNIPVLVVNSAAA

AKVLFGQNAQALSSRPEFYTFHKIVSNTAGTTIGTSPYSESLKRRRKGAA

SALNRPSVESYVHHLDVESKAFVAELFKYGNGGKTPVDPMAMIQRLSLSL

ALTLNWGVRVASQEEELFDEITEVEDKISKFRSTTGNLQDYIPILRLNPF

SSNSHKAKEMRDRRDKYLSSLNRDLDDRMEKGTHKPCIQANVILDKEAKL

NSEELTSISLTMLSGGLDTVTTLVAWSISLLAQRPDIQDKAAKAIQEMYS

EGQPLCDPADDQKCAYVAALVRECLRYYTVLRLALPRTSIRDITYDGKVI

PKGTVFFLNAWACNMDPEVWSDPDEFRPERWFEQPDAPMFTYGMGYRMCA

GSLLANRELYLVFIRTLNSFRIEPTEEKIEWHPLKGNSDPTSLVAIPKKY

KVRFDLISELIREYDTR*

>CYP503B1 Aspergillus nidulans AN1598.1 45% to 503A1 in 54 clan

revised 7/18/07

MDNYTWHSGTLIPSDSPSSIDRSQLYLEILGVLSVVYLLQT

LVAYSKSFKAPFVGFRFWYEPKWLVGLRFSQGALAQVNEGYAKYKNAMFK

VARNDSDILVIPNKYVEELRSLPDEKISAIRAHIKNLLGKYSTTLILLES

DLHTRMLQTKLTPNLGSFIEVIESELLFAMDQEIPANLDDWQSVNVFHIV

LRIVARISARVFLGVPACRNEEWLQTSIHYTENVFATVMLLRRFPKWMHP

IVGHLLPSYWAIHRNLRTAKRIISPMVRQRRAEEAKRNPDYVKPNDLLQW

MMDGANENDGQPDKLAHRQLLLSLASIHTTTMAAAHCFYDLCQHPEYFEP

LREEINDVIAQDGGWKKTTLNKMRKLDSFLKESQRINPPSLLAFNRIVSE

DLTLSDGTLLPKGTHFSMPSAAILQDNGVEPGADQFDGFRYYKKRLNPEE

ANKHQFAMTDNNNLHFGHGKYSCPGRFFASNEIKIIMAHLLTDYEFKYPR

GATRPRNLTADENLYPDPSARLLMRRRVVAPPQASITPQLVSA*

>CYP620E1 Aspergillus nidulans AN1601.1 43% to CYP64A1 64 clan

65% TO 620H3 name needs revision 620H3 = 620E4

revised 7/18/07

MAPTAILITVPLGLLLYLLFIRPSIAKKQKAPLPPGPPPKPLIGNLRDLP

SPDQKNWVHFLQHRDLYGPISSLTVFGQTIVILNDARVAFDLLEKRSNIY

SSRPRMVFAGEMVGWEHILAMQPYSDMFRAYRKAMHRVLGTKNVIAQFNE

LQDVEARRFLLRVLEKPGDLVQHIR (2)

TETGAVILKIA

YGYDIEPHGKDPLVALANESLANFAVAGTPGAWIVDTIPF

MKYLPSWFPG

TGFKRTAASWKQTLLTTIEKPYRLVLKQLESGKYPDSYLSNLLEETKGRP

LSADEEQVIKWTAGSLYTGGADTT

TVSTLSCFFLAMALYPDVQRKAQEE

LDTVLGSAKLPTFGDRARLPYIEAIVKEALRWHPVAPMGIPHMSTEDDIY

EGYLIPKNSLIMPNIWAFTHDASHYKDPATFNPSRFLGDTPEPDPSTLTF

GFGRRICPGRLLADSSIFLTIAQSLAVFEISSAGEDAAKAEFLPGVISHP

VPYRLDIRPRSKGHEDLVKRIAAESPFGEGGAKELEEIVV*

>CYP567C1 AN1737.2 AACD01000027

47% to 567B1 53 clan

MALESILSLPAATLAAGVAALVAMYWLASYAYLYWFHPLSKYPGPPLAAV

SELWYASAWTGGLWNRKIEEAHRRYGDIVRIAPNELSFATAQAFRDIYGA

PSKTRKLFPKSDRFYDNGHPNIAFVLDPEEHARQHKIFAPQFRPSAVRTQ

EPIVHAHVDFWVAQIAARGRSGAVPVDVSKWFEWLTFDIIGELTFGQSFN

ATRDNKSHPWVSILLDATYSGSIFNLRKRLAFVGPLLRWMPYISSTARAA

VESVSQHGAMTLAFTRKRIESGPARNDVDDFLAHAIRAGGMTDTELADQA

MVLLTAGAETSATALTATLWYLSQPAHAHCLERLRTEVRGAFASPADVTG

DATARLPYLNAVLEETMRLFPPSPVGPPRISPAGGETVDGTFVPGGVYVS

ADVWTIHHDARTVGARPDAFEPERWCDGQKPYTVPFSIGPRMCIGVTLAW

VEMRIALAKAVLAFDWELAEDAVGGSGDWVEEARLKQLWQKPPLMMRFRP

LEAKA*

>CYP666A1 AN1748.2 AACD01000027

poor match 32% to 588B1 534clan

Genomic seq AACD01000027.1 revised to add C-term

Intron boundary at VTPLC is AT not GT, but this

boundary is GT in CYP666A2 and CYP666B1

MLVFTIPLATALIYITHSAFSFLSNLYLVCKTCPSLPRVLFPVSEVNLLY

LALFESRWFNHIRRYWLPTSIADYIADAAFRGRWDVKDRLARKYGGFYLF

VAPGLISCHVGDAEVVSQVVKERKGFVKPVKHLEAFEMYGRNVLTSEGSE

WAYHHRYSASAFSDKNNGLVWQESAIQAQEMLAYWEKKFNASNGSDRFTV

PDVREDILKLSLNIICSAGFGVRLPYRPATSATAERDKDSAEDLFRDAAT

PASGYHFTFRGVMEYMNRSMMSVFIANGILPKWIPRMLVPFFKTDFAAHE

DLKKYLHALIEVTENSEHERHNLLGRLVAARREEQSVRCSGSGPGLSNAE

ILGNTYIFSLAGHETTATTMRFALPLLAIHQDVQDRLYKELEEALRDQPA

NPAEWEYSTVFPRLVTPLC (0?)

IMLETLRLYPPVVSIPKMTTTHMAEITYKGERHCLPPNVRVNLNANVLHYSEAYWGPDA

DSFDPRRWDKRNAGSFLARNASARGLSGPGLESPNVHKPVRGAFIPFSDGLRACMGRKFA

QVEFIATLAVIFHRYRVTLVRVGQETEDDARKRVEKALRQSSTLITLALGGVVPLAFVRR

TGVAAEDDA*

>CYP682D1 AN1794.2 AACD01000028

38% to 551A1 38% to 552 53 clan

revised

MLEKGGFLRLFEGAFPASSKSQKLQKPSKSRMLHLVPYIFLAWVAYVVSL

VIYRLWLSPLAKFPGPKLAAATLWYETYYDAFKWGKYTFEIAKMHEKY

GPIVRISPHELHINDPDYYEVLYSRDSPRNKYEYFTK

QFPVAGSSIATVDHYHHRVIRSNMNPYFSITRARKQEPLVQALVNKLIER

LQAYKGTQTPINLQHAFTCFATDVVSDYSMGVGFHYLDEPDFIPDWSDTL

AWSVKNGVYLKAFPWSGALLNSLPPWLLSRIYPGMGLVFQFQDRCNRIVQ

RIMEEQKERGYEEVKNQFDHPTFFHDVLTSNLPKELKTPDRLAREVQAVI

GAGSETTGKMLTWTMYYLLENPDKMNALKEELNRLDPDQTATLVDFEKMP

YLTSVMLEGLRLSYGSSSRLQRIAPDRDLKFKEWSIPAGTPVGMTSVLIH

HNEEIFPDSHEFIPERWLDPEKRKYLEKYMVSFTKGSRQCVGMNLAKSEI

LLCLPHVVRKVNMELYETTRDDVTLAHDLFLGFAEEGSKGVRVLIR*

>CYP617D1 old name = CYP617B2 AN1884.2 AACD01000029

38% to 547A1 46% to 617A1 547 clan

MAPIPWGMLYVSVVGYSVLTSNLFTFFVLATALTALKIVYASFLYPEYLT

PIKHIPTPPLTAQKRSWVRGNTDTYVVISPFEGMLNWTKSVPNNGLLRYY

IIGNIEQVLVTTPKALSELLVQNAYDYQKPESIRISLARIAGEHGILLVE

GQEHKRHRKNLMPAFSYRHIKDLYPTFWAKSVEMVKCIEKDLQDRRDTGD

ITVTVRPWASRATLDIIGLAGMDRDFGSLADPQNELAAQYHRILEDPPLW

LKLLFAAAFVLGNEELVMALPVKRNRDIAEGAKYVRQVAQQLISEKRERI

KHNPEKAEGGGGGKDILSVALNSGNFTDIELIDQMMTFLAAGHETTSSAL

QWSVYALCKHPDIQTRLRQEIRSNLPSVSSNDPKPITAEAVDSLPYLHAF

CNEVLRFHPSVPITFRTTTRDTTLAGTLLPKGTQLTISPEVINHDPDLWG

PDAHIFNPDRWLGPGRANTGGASSNYALMTFLHGPRSCIGQGFAKAELAC

LVAAMVGRFEMELADPGKKLAVRRTATISPVDGVVARLTPLDGW*

>CYP51F2 AN1901.2 AACD01000029

64% to 51F2, 60% to 51F1

MLSPTLFSAYVLVGAIVAVLVNVIRQIFFRNKNEPPMVFHWVPFVGSTIS

YGMNPYKFFFSCREKYGDIYTFVMLGKKMTVYMGVKGNDFILNGKLKDLN

AEEVYSPLTTPVFGSDVVYDCPNSKLMEQKKFIKFGLSQSALESHVPLIE

KEVLDYIKTSPRFKGDSGVLDAPAAMAELTIYTAGSALQGKEVRKKLTAE

FADLFHDLEMGFTPINFILPWAPLPQNRKRDIAHARMRETYMEIINQRRK

NPDAQDHDMIWNLMHSTYKNGNPVPDKEIAHIMITLLMAGQHSSSSISAW

ILLRLASEPQILEELYQEQLANLKRDPRTGAFEPLQYKDLDLLPLHQNVI

KETLRVHLSIHSILRKVKNPIPVPDTPYIIPTSHTLLASPGATALSDEYF

PNANMWDPHRWENQRPDKEDEEGELIDYGYGAVSKRMSSPYLPFGGGRHR

CIGEKFAYVNLGVIVATIVRNLKLYNVDGKTGVPATDYSSMFMGPMKPAV

VGWERRFPARS*

>AN1967.1

linoleate diol synthase not a P450

MGEDKETNILAGLGNTISQVENVVAASLRPLPTATGDGTYVAESTQTGLA

KDLSHVDLKDVRTLAEVVKSAATGEPVDDKQYIMERVIQLAAGLPSTSRN

AAELTKSFLNMLWNDLEHPPVSYLGADSMHRKADGSGNNRFWPQLGAAGS

AYARSVRPKTMQSPSLPDPETIFDCLLRRKEYREHPNKISSVLFYLASII

IHDLFQTDPKDNSVSKTSSYLDLSPLYGNNQDEQNLVRTFKDGKLKPDCF

ATKRVLGFPPGVGVLLIMFNRFHNYVVDQLAAINECGRFTKPDESNVDEY

AKYDNNLFQTGRLVTCGLYANIILKDYVRTILNINRTDSTWSLDPRMEMK

DGLLGEAAAMATGNQVSAEFNVVYRWHACISKRDEKWTEDFHREIMPGVD

PSTLSMQDFVAGLGRWQAGLPQEPLERPFSGLQRKPDGAFNDDDLVNLFE

KSVEDCAGAFGASHVPAIFKSVEALGIMQARRWNLGTLNEFRQYFNLAPH

KTFEDINSDPYIADQLKRLYDHPDLVEIYPGVVVEEAKDSMVPGSGLCTN

FTISRAILSDAVALVRGDRFYTVDYTPKHLTNWAYNEIQPNNAVDQGQVF

YKLVLRAFPNHFDGNSIYAHFPLVVPSENEKILKSLGVAEKYSWEKPSRI

SHPIFISSHAACMSILENQETFKVTWGRKIEFLMQRDKHQYGKDFMLSGD

RPPNAASRKMMGSALYRDEWEAEVKNFYEQTTLKLLHKNSYKLAGVNQVD

IVRDVANLAQVHFCSSVFSLPLKTDSNPRGIFAESELYKIMAAVFTAIFY

DADIGKSFELNQAARTVTQQLGQLTMANVEIIAKTGLIANLVNRLHRRDV

LSEYGIHMIQRLLDSGLPATEIVWTHILPTAGGMVANQAQLFSQCLDYYL

SEEGSGHLPEINRLAKENTPEADELLTRYFMEGARLRSSVALPRVAAQPT

VVEDNGEKLTIKAGQVVMCNLVSACMDPTAFPDPEKVKLDRDMNLYAHFG

FGPHKCLGLDLCKTGLSTMLKVLGRLDNLRRAPGAQGQLKKLSGPGGIAK

YMNEDQSGFTPFPSTMKIQWDGELPQLKEDF*

>CYP551A3P Aspergillus nidulans

AN5460.3

About 47% to CYP551A1

VTFYCASLIIYRLYFHPLAGYPGPKLAAATRW

YEFYYDVIRQGKYVYEVNQMHDKY ()

PIVRINPHEIVIKDPEFYNQIYVAGNTRRTEKWXXXXXXXXXXX

GSIVMTVGHELHRLRRKPLDPFFSRMEIDRLEPLIVKEAKLLNHRLQSYSGSGRVLRLADVF

SAFAVDMITHICSEAGPVMMNKPEFGKDW 224

XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX

XXXXXXXXXXXXXXXXXXXXXXXXXXXXXX

ETKNSLFRQLLSPSSA

GGLPESELDAERLASETMVLFGAGTVTVTSTLSLICYYILRDSHIRDRRAGELEGVMAQYPTSMPTWQQLER

LPYLHALVQEGLRLSYGTMRRLPRXX

PDAALHYKQWTIPP (frameshift)

TPVGMAAYSLHTDPEVYPDPFEFL

XXXXXXXXXXXXXXXXXXXXXXXXXXXXXX

(Missing PERW and heme signature)

LAYAEVYWALAVMFKSNAPKLQLFETEKMDVEHVVDSMVALLRLGSQETRITV

>CYP552A2 Aspergillus nidulans AN2040.1 53% to 552A1 50% to 552B1

AACD01000032.1 revised 7/18/07

MTISAETVLRLLPLTAGGAWVFFHLAKCIYNLFFHPLSHIPGPWLAAATY

LPEFYHDVVRGGRYTTQIQQMHEKYGPIVRINPHELHCNDHRFINEIYAG

GTRKRDKPVHQVTGSEAAAHATFSTTDHDVHRMRRNALAKFFSRAQVLRL

EPTVRGLAERLCDKMLRTEKGRPFHVGAAYSQFTADVISGYCLGENLGLM

GQEGWEEQIVDEVATVLRLGCTLRFVPWWKHVLGVVLLIRKQLVGSPKES

SDALIVGMPNHIRKAQADIKIMDGKGNGNGPETIPTTVFTSLLQSDLPPQ

EKTFERMTSEGISLFAAGTATVSWALTVATYHLLTKPAILQRLTAEVSDL

VNSSSCTEKVEWSALERLPYLGAVIQEALRLSYGVASRTARVATGEDLVY

RGEWAHREVQYIIPRGSAIGMSAVLAHHDEAVFPDSHLFLPER

WLDEKNQRLKELDRALLAFSKGSRGCLGIK

LAYCELYLLLALLVVRVFPRMRLYGTTEADVAYDH

DFFNPFPVWESKGVRVVV*

>CYP532A4 Aspergillus nidulans AN2125.1 56% to 532A3 top part

revised 7/18/07

MALVTEILDHTEPLSNYGLYILPFFILLYLAQQYFHNGLHRYHGPILAKF

TNLWRFLDVRGRRPELTHIALHRKYGDVVRLGPNTLSFASPSAIKVIYGL

NKGFTKANKSQSEFYPVQMTVSKGEPLPSLFSTLDESFHANLRRSVNHAF

SMSSLVQYEPMVNQTVELFLDQTAALFANSGKTCDFARWLQFFAFDVIGS

ITYSKRHGFIEKNEDIDGILKSLARIFDYAGPVGQMPWLDKWLWKNPIYD

VLQRWGIADNSHPVAIFARQRMNERLMADNGTRTEAEQQQDLLTKFIQAG

KDRKDFMTEKRVLTMAVSMAFAGSETTAISLAAVFYYLLKNPEYMARLRG

ELDNAVAHGIVENRSSGLVTWSESQKLPFLDACIKEAFRIHPAAGLSLER

VVPASGIDIAGHFIPGGTIVGCSAWVLHRREEIFGPDVDSFIPDRWLNVS

AERLKTMNGTMLQFGAGARTCIGKNISLMEIYKLVPSFLRRFDIQLAHAD

QEWELWNAWFVRQYNFKTKFTARNFEV

>CYP630B3P AN2191.2 AACD01000034

55% to 630B1

pseudogene very similar to 630B1 AACD01000034.1 ANcontig1.34

note 630B1 is missing 16aa after the CYS 53 clan

MLEPILALLLVYSFYSYLVAPIITYIWDPKNLRRYPNFHPLPGISDIPFLLAAQKVF (fs)

RSRTLHALHQHHPIVCVGPNALSYGAPTAIKDIYGHGT

TCVKDRFYSEKSGSHAHLANVV

GKQDHARKRKVLASAYAIKNPEG*EYKLSGMTARLMRV

FEGKCTEPLPSSV*ATEERDLTIDFRSLTNHFT

VVAIANIGLTEDL

GFHEQGSDTNSLESMDGRVKDVSFKECREASGTVAYRLIWAYDWFPVLKR

LSKVLSPYYRKLGKLDADWNGIVYNRATRRLNV (fs)

GEKLDDFFTAMMEDKKGAAHNLETGEIVAEI

SIMMNAGSDTTAIALRNVLFLLLKNPRCMARLREEINVVLDEDELVAPYS

KVKHLPYLRACLDESLRMLPPVVFGLPRRTPPEGTTILGEHVA (fs)

GDTSVSMSAYVVHDESFFKDNSTYLPERWLGE (fs)

RSKSLQSYFIPFSARARGCI GRNISYL*QTVVLASL VHRYDFALP (fs)

XXXXXXXXXXATNLSPGPMPLKFGRGFMHDE*

>CYP646A1 AN2596.2 AACD01000043

54 clan, revised 2/21/06

revised 7/18/07

MLLELDVSLFSMAALVGTLVCIVVKLFETSPVPKDIPWV

LKKQNVLRRAAERFIGVNPISFIQEGYEK (0)

YSKNQQPFVMPSVNEGDEVVLPREHSNLVLFAKES

EYSFKAHISDFFQLKYTSWPLAFAEKYDFFVKLVSKDLTETLKSDAIAEA

LANEARACLSDIWGEDEDNWVEVPLYSAMEN

HDTSLLNAMTNCSNAIVFGANVIKAFPSFLHG (2)

IVGPIVGLVNRWQERVFHTRMKPLIEARIKTQREAVDKAALQENLKAQGSLLDMLINAGLRSKW

PVEVETMWLAYRIFMINFPGVHTSGISATSALLDILSFPSEEGLIDMLQAEIQNIAAS

SCGKWTAAELEQASLLDSAIKESLRFNGINALSPTRM (0)

VVAPEGTTLPNGLFLPYGTKI

GIPQYAVHRDSDLYPNPDQYNPYRFYTPGASPAQLRESSMTNTSESYVVFGHGRRQCP

GRWIFAHIFKLLIAEMLLKYEIKPFPTRPKIH (1)

RWGRFQLPPLTTKLTVRRRKDTA*

>CYP661A1 AN2607.2 AACD01000043

30% to CYP56A1 547 clan = 56 clan?

MHPTLLYTCTFFITVTALLWRYFRVPSTIPSNIPRIPIWVNFYAWYHDLS

VIELYDAFYRKIMEEHGAVVVWFTGAWCILVTKPEYLVEIFRNEDTYPKV

GVNVRGKGSLMGIFAGENIINSSKPTWATLTKVMKPGFLKSFDAQAIHAK

AKKVPERLLQAQSEVGKGKGVPATQWMEKYAQDVMGLCLFNFDLQALDEP

RVPYAPLLGQIIPTIFSRWAFYFPTLDIPGRYFLSRKATLDNIAKFDSLL

NEIVESTLNADAEKQPKVVSHMLKRALDNGQLTPELYRYNLRMSFMFGHD

TTAIFMGLTMYVLGNNTVLQDRLRTEVLQSTGKTIHQLPYLTSLLYEVLR

LYPPVTEVLNHTVSRPTALGGKVTIHPGTWLGWNAYGVHTNPAIWGPDAL

EVRPERWGDTVKDIQASFRLQSTKGNYIPFSLHARKCLGQSLVLTEVKLL

MFEMVRQLKWVVDPTYKLNLGGVTFTMLLGLRLIVEELELDSRDTVADEK

SQMHT*

>CYP566C1 Aspergillus nidulans AN2610.1 38% to 566A1 53 clan

revised 7/18/07 NW_101284.1, 79% to 566C3, 79% to 566C2

MTLIESSPAQFTLVACSL (0)

GVAFLVLIVVYSYFFHPLARVPGPWTGAVFP (0)

309588 FWSISTLYRRRLNPDLAELHKIY (1) 309535

309472 GPVVRVGPNQLSFATIEAQKMIYNAKPTNNGSDEH

FGRDGTLQDVLLSMILGAPNIGSLSNRAEHKKMRK

RLLPGFTSNTIFEQEPLLRLHVDQLLHRLAQDAGLINLTSHFSRFLWDLI

GDFSFGEPLVPEKH (1)

ELRTDKLKSMVEIYQGCFPLLEAINHVVPEINAVIRLAFRLVPPATLRAVLPTATLRG (2)

CVDRQDGRSDFLTHIMGDKSRDPTELEL

SYDEVHSNATVLM (2)

IAGYKTTETSLSALFYRILSTPGVLKNLQSELFSNFQ

SIDGITGRKLLSLPYLNGCIYESLRLAPPVAGKFASRRSPGAVIQGFYVP

PGTEVFTETYTMQRSPLYWYAPDEYHPERWLKQSEGSPYAQDVHEAFKPF

SSGPRACLGREMALQTLRLTTALLAYRFDMEMVNEDQFVWEQDTESRMVY

SQYQIKAVVQEHLT*

>CYP56B1 Aspergillus nidulans AN2706.1 43% to CYP56A1 547 clan

revised 7/18/07

MLVTLTVLFLGLVGILASFLTYLFTPPQFFPKGLPTIPFYYTLIPLLNQT

LKRAPTDQVTLYHRYLSTPLRTHGAVKLFFGGRWNILITKPSYIAEVLKN

EDLYAKSGNQKKIPHSVLAQYTGDNIISSHGENWKLYSSIFKPGLQRDYD

PSGIWRNASLLVQMISQDIKRKSPVDINPLMQRYALANLSEVLLGTTFDT

LQKPAASLHAFQLLIKPKIFDPIFLNFPVLDYLPLQTREEARKLVTRFTD

ELIETVRKGHTTCDHEKEHTRNLGCRLLYACESGLFTEMQLRHNMISAFL

AGHENPQLLLVSSLFLLAEHPEMQESLRAEISALNDLEPAYNALSALPLL

TSTIYEVLRLYPPISQLINRRTTAPTLLGGEIPIPAGTYVGYNAYATNRD

IGFWGPDANEFKPSRWGNTMEEINALFRRANAKGAFISFHGGRRTCLGQR

FALLEGRVTLAKLLMCVRWEIDPSWQRGMTPAGPLYARNLQLRFSNISGAGKAGAS

>CYP665A1 AN2727.2 AACD01000048

35% to 530A2 66 clan

MGLSLGNDLWILLVSLLVAIVTSLVVALAPYTWASLRPKNFPPGPKPLPL

IGNLNLIPPSKAFLLFHQWTKKYGPIIGLKFGPTNVVVLNNWKDVQELLE

KRGHIYSSRPDNYIANELICKNHTHILFAPYGDGWKALRKAAQGLFTPRE

LAGVLPIQEAEATQTAFDLIRSPDRYYEHIQRYTTAVILASVFGQRGADF

NSPNVQALYDVQNRFTALLEPGAAPPVDGITFLRHIPEFLAPWKRRAKEI

RRDQRALYFRLYNATKERMAKGIRTGCFMEKLIDDQVKNGLDDEHTTYLG

GILMEAGSDTTSSTLLSFLLAVLENPGALKRVQEDVDRVVGTERSPTMND

LENLPYIEACMHEILRWRPVAAGGIPHMLTQTDTYKDYIFPAGTIFFANT

WAIHHDETEYSNPAIFNPDRWLDGNKYGTKDNNSTLDSTAQRKTSYGWGA

GRRICSGQKMAEASLKINIAKLVWAFDFERVKGEENVDISVDTGYEGGFL

VCPKKFPIKITPRSEGRAAVIEKEFEGLKGFYEKLAA*

>CYP631B1 Aspergillus nidulans AN3225.1 38% to 631A1 53 clan

revised 7/19/07

MDHPHPSTFSL

GLSQILVCLALLYAAIHILSV

YRRLCHISGPFWARIS

NLPRVWWVNTSRAHEIHQQLHEKYGDVVRFGPNMVSLRNPTWIPTVYPTR

MGVKKSDFYRTLAPYTPSGALPAVFSSRDEEVHRGLRGPIASLYSMSKVL

PLEVFVDRTIDVLVRQLDGRFAGAGETFDLASWLQFFAFDVMGTLTFSKR

YGFLEKGMDVHGMLDTIWRFLKGAAPFTQIPWVDEIWNKNVLATKLKGAT

GVSILGIVGKFVSQRQEESKAGKIDGTADRDMLSLFMEIQKNNQLPPWYV

TAWTFSNITAGSDSAAVVMRTVFYNLLSHPSTLQKLRSELLSAGPLTQPY

PSWKDVCNLPYLDACILEALRLHPPFCLPFERIVPQGGMVLGDTYFPEGT

VVGMSPWVVNRHKPTFGEDSDVWNPERWMVSKELKSKREAAVLTFGAGRR

VCLGRHIAILELKKIVPALVLRYDFELIDPERFTTENFWFFRQRGMDVRV

KKRMQAEAGI*

>CYP649A1 AN3253.2 AACD01000054

32% to 503A1 54 clan

MEDPSSLPSLYPGLFVLFLLYFAGDLIATRRAAQRQKDHPLVGSPSWWTP

RFGLNLVFAARAVEILQTGYHKFKNRTFQLIRGDGSVVILPLHLIDELSS

LPQSVASSHGALERDLLGRYTGLDIILTSRMHHTIVQRKLTPRLAALTPS

LQDEVSLAVQEGFPHSTEWTIVKPYQILAQVAAKIAARAMVGPSFCRDPR

WLDISVNYTESLFRTIVILRLFPGWTHPVLSRCLPSYWAGKRYLQRAKGI

LGPKIDELIRRNDTGEWSPERTESDFNVLCWLVEAAKGRDRNAETLAHIE

VLLALAAVHTILLRLVNVLYDLVAHPALFEELKEEIQDIGFNEDWNFGSY

NKLRKLDSVLRESQRLSPPTILGLKRLFLQPYKFTSGITVPAGTYVALPV

MAIENDPLHTDNPEEFDGLRSYRRIEQKTASMRPNPKDGPQFSTIEKTVL

GFGYGKSACPGRYFASLVLKMVFVKLLTEYDFQFLPGRSRPKNYLVHEFL

FPWPWDKILVRRRENGVCPF*

>CYP648A1 AN3256.2 AACD01000054

poor match 54 clan

MFQAASSVLSTLYQICANGAQAPLPAGSVWAILVLLLVALILLVCRYADS

YDGVPVVNRICALEPRVFSRIRFAFNAEQILQDAYRKYDGKPYIIARGDA

DYLVLPSESVTELNRLPASIINSRMCHAYSMTGHLNGMNVVLKSNLHVKT

LLNRITPALPAFLGPASARMQATMQETFPSVSSWTTIEPLDLVVGCVSRA

ITLAAVGEPWCDDTELVNLTFEHTKLVFTVMFAMRLVPAALQPVLVWMLP

HKWRLQKSLQRLESFIVPIVQECKAAKPRPATERPSTLLAWMVAEATNDV

EEDPYVLTELLAALAAGGTYSSANFIVSVILDLIANPQFLDEIREEIRQK

HEELQGRWDFAAFNNLPKLDSAFKETIRLTPGSLTTYSRVMLQDYTLSTG

ITLKKGQFICVSSYARAKDDEIYQNAGSYDALRAYNESQQYHAAQPFKGV

YQQEFRWGAGRWACAGRHLASLLAKFIVVKLLDEYEFQFVPGSHRPPNSV

FHEFVFVHPSTRLLTRRREENLGICCW*

>CYP667A1 AN3272.2 AACD01000054

29% to 589A1, 534 clan?

MLSLPSSVFTILVGLFLYKIIRLAHNVRLARKTSLPYIITPLLETEVVAL

LLNPLLRYLYRDYLDRGKGWPRWCRFFVKDWAWEDKRQAHDELGDVFLCV

SPEGIICYSADATMGWDVMNRRNTFTKPRDKYKVLEPYGMNVATAEGKTY

QFHVRITAPPFGDLSGVNNLVWRETVHQTKRLTEAWAESPPTEIQKDVNA

LTLAIISLAGFGKRLEWSKNGEKEGVPAGYQLSFLHALQDTLHYMVPILL

FPRWVLRVILHKAALAHAQLDRYLREIIRDQKAKLSADINHSDKQSRGNL

LTAVVRASMVFDSEQSSKESSRSSGERKQGFTEDETMGNLFIYLLAGYET

TANAIIYGLAVLALHQDIQDQVIEEIDRAHARAQSANRSELTYEDDFEFL

EYTYGFMYETFRLFPGVTLITKMIHQPERIITDGPDGSPKSYDLPAGTRV

YLNAPVVHYHPKYWPEPYKLDPNRWRSSQGEKHVVASDRTRQMKGTLLTF

SDGSRACLGRKFAQAEYVAFFATLLRQYRVRLAPGSDPAVVERDLFGKSA

GTITLAPLANVRLHIQPR*

>CYP654A1 Aspergillus nidulans AN3274.1 35% to 512A1 54 clan

revised 7/18/07

MILKLIPVTGGAALLLQASALLSFAWLLLAWASRRRRNRLIPGVYVAGLKSGKVPLSQARQAFIHGCA

DLMLEGYQKTQGGLFYVPSPAGERLMIPTKYLDELKN

AANEEVDFTASFSEMFEGRYTTIGQKWHLHPDVVKKSLNANLELIMPDVY

DEIVHAYRSLLTPSHDWQPVRMSEIFTQIISRASNRMLGGKALSRNRDWT

DTSINFTTDTWLASQQLKRYPAWLRPVIQHLLPEMGRVRRHFTVARQVIC

PIVQKRSESDNDTKKPLDLLQMLWEGAEPVDQTPEFMAYTALAISFAAIR

TSSSVPTHLLYDLCARPEYIAPLREEIESVLREEGSIFTKAALNKLLKLD

SFMKESQRFNPLSLLTFGRVIQSDRILHDGLVIPKGTIIGVPAHAISQDG

DFYPSPSTFSPFRFVPSAPGEKTAGFVTTNASSSLSWGYGKHACSGRFFA

ANEIKLIMAYFLLNYDFQFAGGRTERPANYTFELQNMPDETVEVLVRRRK

DGNL*

>CYP567E1 Aspergillus nidulans AN3275.1 Missing N-term 147aa 53 clan

revised 7/19/07

MVPYGTCETMDHFRGQPALVLIGASALVGIITWIARLLIRAFVSHPLAHFPGPRLSALSN (0)

LNYSWSYMGGRQPYDILNLHAEY (1)

GSVVRVAPNELSFNTAQSWKDIYAPRKGRATFIKSDFYDGGNFADQASSI

VSERDPARHSAMRKFLSAAFSDRSLREQEGLVTRVIDDFIDQVGQRGNSKDGVDMTMWTNL

LTFDIIGEL (1)

AVVLESMGQAGLSDFLKRFPIIGRVFLKLNPRWLNKLMDGAIKHQTYTIDLVKR (2)

RIQQKTNRKDFMSYLLLERNSSQISDIQLAAHASDFV (2)

ATLKEDHSIAGSETTATCLATVIYYVGRNPRILKTLQEEVRSAFRNYEEI

NGQSTSSLKYLHAVCLEALRIFPPLALALPRVVPEGGEMIDGHFVPEK (0)

TIVSTNPLAASMDPANFDTPWAFCPERWIRLSEKDQLEASQPFSMGSRSCLGRG (2)

LAWLELRLTLAKLYYRYDLKLVDDELDWHRDAAMHLLWVKPKLMTQVLPRAK*

>CYP567D1 Aspergillus nidulans

AN3281.1

41% to 567B1 53 clan

revised 7/19/07 89% to A. clavatus CYP567D3

MSASWVDEPSSLLGIFEVVFVL (0)

KRHPNGSELQTLVYVSTRILYNIYFHPLAAFPGPLLARASM (0)

AWRIYHSMGGRFHRAIDEQHRRY (1)

GPVFRVSPNELSFASVASWKDIYGHRAT

GKPPLIKSKFYEIYGAGFGSLCIGSERDPEKHTRMKKSLSPAFSLRSLGD

QEVIVSQCVDRFVSRMGEPELNAGGLNMTKWYEMVAFDILGEL (1)

AFGESFHSIED (1)

GKPHFWSSLIEDHLYFITVADNFRHFPVVALIAKLLSPFLDSIRKKHTGYTRNKVAQ (2)

RIRSSSPRKDFMANLIGKVESNEMSREELTAHASTLV (2)

IAGGETVATFLAATTFYLLRDD

AVYQKLKAEIRGQFNTYEEITCATAQNLPYLQAVISEGLRIYPPGSQGFP

RISPGASIDGIWIPAG (0)

AEVYTSAWTVTHDEANFHDPHTFKPERWIDPDCADIKEASQPFSLGPRACLGRN (2)

VAYMEINLILARLIWTYDMELVNKSMDWESESSLHVMWSKPELKVRFRQARP*

>CYP659A1 Aspergillus nidulans

AN3349.1

poor match 25% to 640A1

lower half 7 clan, revised 7/19/07

MDILDVASSELYTLVRSLVTSPVCQGVLFSLLT

WRLWRFTISPTLQPEDPKEAPYWIPCI (2?)

NQLGNTREPFALTLAGKTMYIIADPKDAAEL

IRNSHSLSTLTTELYTRMGIPQAVIERLFTVYPDAPFNARSNARPVHATD

AMIEMYRAHLSPGAQLDEFLERDVTKRILNAFAKIPGLFNK ()

GIVGAYYGDLIFSLNPGFIAQFMVWEKVNWKLLFGLPSFLSGDMLAARKGLVDGFVAYF

ALPRAERGHENYWVKGVEDSLRWLNVSNEDIARIFMLQTWA ()

ILGNMYKMTFWLVAHILHDAALVNAITAEVRPA(0)

IEIDHHYLSEQCPKLDSLFSEVLRLTLTAPMARDVSETTTVGGRRLREGNRVL (0)

VLYRQLHLDRAT

WGPTPQTLQPDRFLIDSGLKSSIAYRPWGAGKHICPGRFLARSAVFTFVA

YLLAGFEVRLRETGTGKSTRSAFPMADMSRPSPGIANIADGEDVLISVIRRDRSS*

>CYP620D1 AN3394.2 AACD01000055

47% to 620B1 64 clan

MTYFLLILTPDSTKMAIASLILWGIGTLICYLIYQHSTRSAQRLPLPPGP

KPLPIVGNLKDFPPDGKPEYQHWIHHKDRYGGISSVTLLGMTLVIIHDKK

AAHELLEQTSGRTSGRPTMVMANKLCGYESIVVCQDYNARFRRCRKFLHQ

ELGTKVSASQFSAAQELEVKRQLVRALNEPEKWLEHFKTTAGATVLKMAY

DYAVDYHKPDPLVDLIDRMMTEFSLAAVPMAWAVDIIPALQYLPGAPFKK

TARKWRKSIQAAAYIPYRFVQSQMAALTYKPSYVSKLVQLLKGEQSELDH

EDEQAIIWSAASLYGAAADTTVITLTTFTLAMILFPDVQRKAQEEIDRVV

GNRLPGFKDREKLPYINALVLEALRWWPIAPMGFPHTATEGFEYNGLYIP

KGAYLLPAVWWFLHDPEVYDNPEIFDPDRFLEPRNEPTPMTEAFGYGRRI

CPGRFFADSSLFLNIAQSLAVFNFKKAVSSDGKEIEIDVKPKPGLLTYPT

KFDFRIEPRSERHIQMIRELERQDPLAAGDAEHLESIDNFQPL*

>CYP623B2 AN3497.2 AACD01000059

53% to 623B1 54 clan

MHIMNAVDSLLGVGLYFLLPAALLYIINTVFFGAVYPRGVPFIRERPGKT

SFSLKTRLAYLTDCESLFREAYHKYLKNGKPVILPGFGVRTELILPVSSL

RWATTQPDSVLSPGEAFVEIDQADYSLGHSRYVVDAWQGMLVKNEMNAVL

ENIVAAMNEELGVAFDRYFGTDEENWRDIDLLEAAKLVVAQAASRFTVGL

SLCRNEKYLNDSFDAIDGCVITAGVVGGCPPVLRPLIGPIAGLKARLANR

RLRKHIEPLYHARMEALKLPIEDQPQDHFQMMLRFAQRERKEELYSLDQI

ATRVTVANFGSMHQTSIQVTNMLLNILASDPEYNTIAVLRDEIDQVLGSD

TAWTKAKVSKMTKADSVARETLRCHSFGGRAVFRKVLVDGVTTDTGLPLP

KGTLFSFLSQPAHVDEETYEDPFKYDPFRFSRVREAANDPAKAGSLSFVS

TGPSYLAFSHGKHACPGRFLVDFELKMIIAYVLKNYDIRFPEEYQGKRPA

NRWVAEAVFPPSGVRVLVKRRRA*

>AN3536.1

very poor match similar to Trichosporon cutaneum phenol hydroxylase

not a P450. heme signature not conserved in phenol hydroxylase

MGDTVRTDVLIVGAGPSGLMAGVWMAQMGVNTMIIDQKHNLTRCGRADGL

ESRTLEILDSFGLADKIWTQANHTVEIALWGAGADGRLQRQSITANSKPG

WSRFYESTLSQGQVEEYLMQFVRARKHVEVRLETIPTSLEIDNMTIDHHD

AFPFRVNLETAPFSPQSSFDGVATPNSELSSGQSDDSGYAGMGTMVEAKY

ILGCDGAHSWVRKQLGLKLEGETYDDCWGVLDIIPLTDFPDIRKRFIIKS

MHGTLMMIPRERKLVRIYVELPFEAAERYRAEHNAGILMEQVATIMKPYS

IRTNYVDWSTINKVGQRICRKIGPHNRIFLAGDAIHTHSPKAGQGMNVSM

QDTFNLGWKLASVIKGVLHQSALETYQQERLPVAEQLIALDQRICRGMCT

KRNGGVEAQNGRFDEDHRRALEQENSSMSGLAVTYQPNSLVTPTVWDTSL

PSDVPPCTSRPSVARNLRVGARVPSVLILNQSDSQACHLHQILPSTGQWN

LIVFGGDIVAANQKRRVAALTDALNAPDSAFAKLNNRVSIAQRGFPVLGM

YLIHRAHRHSIELNDLPLVFRPWTNDGVDYGRAWVDAETYHHAGGGKLYS

SFGIGPKGCMALLRPDQHLAFLSDIDDVDGLELFLRSVMPGAT*

>CYP681A1 AN3609.2 AACD01000061

34% to 551A1 53 clan

MSFLGLLLSAVFPVAIYGIFLVVYRLYFHPLRRFPGPKLAAATFWYEVYY

DWFKGPYPGSSWNLDRLHNQYGPILRKTPDELSIRDPDYVDVFFAGGRRD

RYSRQGKEAQGSVQSTLLGSDHRRRRGALTGFFSKRSLDTLEPFIMDKVE

QLSASVEENFLKTGNILEAGVAFGALTLDTITDYCFDQSFGCLSKPDLAP

EWRRTFWDMLESIPFLKNWTFFAEMFFWVPQWVVKHTNPAMEQFFIMQAA

IRAKVARVTMEWEQDQALQLQGKDPFMKGKRKRTIFYDILNSAVLLPEDK

TPKRMAEEAFGMVVAGGYTTGKAMANLMYHLHANPKWLERVREELDSLMP

SPDQPVKLSDLQALPYLTACIKENLRISNIITDSIMLVEPVDTLTYKDWV

IPPKTPIGMTLYHMHMDEQIYPEPKAFKPERWIKGAEAKHDLDKYFAPFS

KGTRGCLGVNLANAQMYLGLGVILRRFDFQLFDVVKERDVDTVRDCFVGL

ESPESKGVRLRVMDKRE*

>CYP540B4 AN3861.2 AACD01000062

67% to 540B2 505 clan, revised 7/18/07

MLNQQFYLHGETASSAKSITLDETANLDQVKHIVAAHFAIVEPNGIGFQT

ENDCLVDVSSILTAPGPIAITIDGRAVREPEGPKGLPFVGNYFEVFPDHL

GNHQRLFDTYGPIIKTNNLGRTTYQTNDPQLSAIVLAESDFFTKKINEAH

PLYPLKTPEAGVFLGDTDTKEWRDAHKFLPPALGPKAVRHYAPTMDSCVK

DAFKVFDALDETGEAWNVYQYMLKLGSQAVGKLTLGLDFEHFTSPDAPTH

EMVHAIAELLSLNKEVTSRGDWYAKLPFGAPKRLRNLKARIEEMVDESVQ

RAARGGVSDLPLQEAALQASNMVDYAIRATDNKGEKLPKSSLIWALVVAT

GAGFTTTSSLLSWLIYGLVTYPGMQDRLLQELVDHGITEDTELTADLTEN

LPFLDKYIKEMQRRHNPSYQPGRTAKVDLVLPGGYKIPKDSVVIAALHHI

HNNPSIWDNPSRFDPDRWDTDEVKQRHKASYIPFAIGPRMCIGFNFALSE

IKVFLPRLVYRYNFIREGDGPIEYDPMFQLIRPNNL

YVRAERRVKWPAKTKSSE

>CYP539D1 AN3917.2 AACD01000064

52 clan

53% to 539A1 N-term 94aa dont match

49% to 539A2 first 43 aa dont match

42% to 52A1 N-term 100aa dont match

MIEELVSKTTPGQAVVYLLGAFFLACLARKIQVQWQLSRLGSRAPKIPFR

LPYGLLIPSISRNSHADRRLSSAMDFIYKSMQANKSDQDLEYFTDVMRNA

KGAPLAEDKKTVELDAGISSRIIFTRDPENIKAILTGQFSDYGKGESFHR

DWREFLGDSIFVTDGEQWSASRHLIRPMFVRDRLVDTEIFEKHVQHLIPL

LAGNNESKIVDXXXXXXXXXX

VTSLFFRYTLDAATNYLLGQGTDSLQNPETEFAEAFRYV

QRRQAQYFRLGVFKFVLSRTEFRRQLKVMDDFIKPYIDRVLAMSPAELDQ

KLSKSDTFLHALARFTRDRRTLRDQLVAILLAGRDTTAATLSFCLFELAR

NPEVTAKLREEIASRLGLGASAQKPSYTDLKEMKYLNAVLNESMRLYPVV

PFNVRFSLKDTTLPRGGGPDGLAPVAVRAGTRVVYSTMIMQRSPENYDPP

SAPNYFDPAQWNPERWLSGWQPKPWQFIPFNGGPRICIGQQFAMIEMGYT

VVRILQAFERIQAVPEAGKSVVEDPRLRFEVTLTPASELNCVFVRDGEGR

EDEKISR*

>CYP61A1 Aspergillus nidulans

AN4042.1

66% to 61A1 51 clan revised 7/19/07

MAEINGSFVSPAADATVFPQVFQPAGLIADFLNGLTLWKTLATLFALAVV

YDQFRYIYLKGAIVGPAWKLPFMGPFLQSVNPKFHEYKAKWDSGELSCVS

VFHKFVVIASTRDMSRKIFNSPTYVKPCVVDAAHKLLGKTNWVFLDGKEH

VDFRKGLNNLFTRQALSCYLPRMEEVYNDYYARFLKKSKNNNYKPTPWMP

EFRDLMCAVSCRTFVGHYISDEAIDKISVDYYNITAALELVNFPIILPFT