This file is in progress

159 different P450 sequences

and some false positives annotated in Genbank as P450s

23 new CYP families not seen in 6 other fungal genomes,

including 2 other Aspergillus genomes.

D. Nelson March 29, 2006

#44 60% to CYP51F4, 79% to 51F1 An

>CYP51F1 BAE60239.1 [Aspergillus oryzae]

MGILAVILDSVCERCSGSSLWMLSTVALLSILVVSVVINVLRQLLFKNYKEPPLVFHWFPFIGSTISYGM

DPYRFFFNCREKYGDIFTFVLLGKKTTVYLGTKGNDFILNGKLRDVCAEEVYSPLTTPVFGRHVVYDCPN

AKLMEQKKFVKFGLTSDALRSYVRLITEEVEDFVQKSSALQGPNGVFDVCKTIAEITIYTASRSLQGKEV

RSRFDSTFAELYHDLDMGFAPINFMLPWAPLPHNRKRDAAQKRMTETYMEIIKERRKAGSKKDSEDMVWN

LMSCMYKDGTPVPDEEIAHMMIALLMAGQHSSSSTAAWIVLHLAASPEITEELYQEQLRILGHDMPPLTY

ENLQKLDLHAKVIKETLRIHAPIHSIIRAVKNPMPVEGTPYVIPTSHNVLSSPGVTARSEEHFPDPLEWK

PHRWDEAIAVSSEDEEKVDYGYGLVTKGTNSPYLPFGAGRHRCIGEQFAYVQLGAITAALVRLFKFSNLP

GVQTLPDTDYSSLFSKPLGNSKIQFEKREPVTKA

>CYP51F1 AF227920 Venturia inaequalis strain Ent27 14 alpha-demethylase (CYP51A1)

MGLLSPLLAXLPGSDRSWLFYTLASFGFTVAIVAANLVKQLLFS

NPNEPPVVFHWFPFFGNTVVYGIDPIKFFAECKEKHGDIFTFILLGRKTTVYIGTKGN

EFILNGKQSHVNAEEIYSPLTTPVFGSDVVYDCPNSKLMEQKKFVKYGLTTE ALKSYV

TLIQQEVEDYTKRYPQFKGEKGSFDVCASMAEITIFTASRSLQGKEVRDKFDASFADL

FHDLDMGFSPINFMLPWAPLPHNRRRDAANKKMTETYLEIIQSRKAEGVKKDSEDMIW

NLMQCVYKNGTPIPDKEIAHMMIALLMAGQHSSSSTSSWILLRLATRPDIQEELYQEQ

IRVCGADLPPLQYEDLARMPLHNQIIKETLRMHSPIHSILRAVKQPMPVEGTPYTIPT

SHVLLAAPIASGGSPMYFPAPEKWEPHRWDEGSGGTNISGGENGGEEKEDYGYGLITK

GASSPYLPFGAGRHRCIGEQFAYMQLNTVLATQVREFKFSLREGESFPKTDFSSLFSG

PLRPAWLNWERREKSS

#43 77% to CYP51F4, 75% to 51F2An

>CYP51F2 BAE57417.1 [Aspergillus oryzae]

MASFTLVSAYAAAGLLAIIVLNLLRQLLFRNKTDPPLVFHWIPFLGSTVTYGMDPYAFFFSCRQKYGDIF

TFILLGRKITVYLGIQGNEFILNGKLKDVNAEEIYSPLTTPVFGSDIVYDCPNSKLMEQKKFIKFGLTQA

ALESHVPLIEKEVLDYLKTSPNFKGTSGRVEITDAMAEITIFTAGRALQGEEVRKKLTTEFADLYHDLDR

GFTPINFMLPWAPLPRNRKRDAAHARMREIYMDIINERRKNPDRETSDMIWNLMHCTYKNGQPLPDKEIA

HMMITLLMAGQHSSSSISSWIMLRLASEPAVMEELYQEQITKLSPDGRTLPPLQYRDLDLLPLHQNLIKE

TLRLHLSIHSLMRKVKNPMPVPGTPYVVPADHVLLASPGVTALSDEYFPNASRWDPHRWENRVEKEDEED

IVDYGYGTVSKGTSSPYLPFGAGRHRCIGEKFAYVNLGVIVATMARHMKLFNVDGKKGVPATDYSSMFSG

PSKPAIIGWERRFPEKS

#42 72% to 51F2An, 77% to seq 43, 60% to seq 44

>CYP51F4 BAE63554.1 [Aspergillus oryzae]

MSWPRIGAYALLAFVAIMALNVTYQFLFRMLNKTRPPLVFHWIPFIGSTIHYGTDPYGFFFSCREKYGDI

FTFILLGRPTTVYLGTQGNEFILNGKLKDVNAEEVYSPLTTPVFGSDVVYDCPNSKLIEQKKFIKFGLSQ

AALEAHVPLIEKEVEDYLAMSPNFHGTSGEVDIPAAMAEITIFTAGSALQGEEVRSKLTTEFAVLYHDLD

KGFTPINFMLPWAPLPHNKKRDAAHARMRAIYIDIINKRRNAGDNVPEKLDMIGNLMQCTYKNGQPLPDK

EIAHIMITLLMAGQHSSSSISSWIMLRLASQPAVVEELYQEQLANLERTGPNGSLAPLQYKDFDNLPLHQ

NVIRETLRLHSSIHSLLRKVKNPLPVPGTPYVIPTSHVLLAAPGVTALSDEYFPNAMAWDPHRWETQAPQ

ENNKDDIVDYGYGAMSKGTSSPYLPFGAGRHRCIGEKFAYLNLAVIVATMVRHLRFSNLDGQTGVPDTDY

SSLFSGPMKPARIRWERRAAKSG

#24 58% to seq 25 63% to 52G1

>CYP52G3 BAE65166.1 AP007171.1 [Aspergillus oryzae]

14 P450 genes and 2 pseudogenes on this contig

MIPLLILAALAVVFRLVWSIFTSLRHAQNARKWHCGAIPTYPGDILGINTLKEVLRADKEKLIPVLSAQR

VETMTAREGRYVSTFRLRQMGRESIFTSDPKNMQAILATQFKEFELGSLRRNSLHPLLGSGIFSTDGEAW

SRSRSLLRPQFTRDQVSDLDLEERHVQKAMAGMLADPATKWTPEIDIQSIFFRLTIDSATEFLFGDSVES

QTAALSGSRIIEDKFPSYFDRGQWYAAQRARFEKLYWIVNNKESRETDRFVHAYVDRFIDAALAAVKEGK

IDPEKRNSDHYVFLHGLTTATQDPVELRSQLLNILLAGRDTTASLLSWCVLLLARHPDIFQKLRNTILAD

FGDYRNPRNITFSSLKSCRYLHYFMNEVLRLYPIVPGNRRVALKDTTLPRGGGPDGSEPVYVRKGQPVVY

SVFVTHRRKDIWGADAEVFNPDRWEDLKVGWEYLPFNGGPRICIGQQFALTEAGYVLVRLLQRFDQIVDA

RPEREIRFNATLTSAPWENVIVRLREGA

#25 54% to 52G1

>CYP52G4 BAE55312.1 [Aspergillus oryzae]

MSDARTTLMETWFTPALVAAIVAARLLFSLYSAWRHAQRARSLNCQEAPLYPSRDPFGVATLLETLRADR

DKFLPKLSQKRVDLISSQQNRYVSTFRVRQAGRENFFTVDPKNIQAMLATQFNDFFLGDMRRNAGAPVIR

SGIFVSDGADWSHSRSLIRPQFTRTQINNLELEERHVQNALRAMPTQSNGWTSEVDIQTILFRLTLDSAT

EFLFGKSCNSQLTALEKDAGEMSDSFLNSFDRCAWYLAARLRFERLYWIVNNKEFRECTRVVHELVDGYV

HAALQRAQQAEKPADPERGSHYVFVDALTATTQDPNQLRDECLNVLIAGCDTTASLLSWNILLLARHPDI

FQRLRKEIIERFGTYSEPRDINFSSLKSCQYLQHFINETLRLHPVVPFNRRCANKDTTLPRGGGKDGNSP

VYLQKGQPVLYSSYVLQRRKDIWGEDAEEFNPDRWYGRKAIWEHIPFSGGPRTCIGQQFAITNTSFVLVR

LLQRFDSIEDVYPEREIRYGVTLTNCPADRVTVRMHQAEL

#35 71% to 52H1, 48% to 584G1

>CYP52H3 BAE64862.1 AP007171.1 [Aspergillus oryzae]

14 P450 genes and 2 pseudogenes on this contig

MLAQIAPLFGALCITFLVLQYLQRLYQQRKRAQSLGCQPAAQGPSGIFGIRSFVRLLNEVRHKRWVEYIA

GQYGRYGNTYTQKALGKWMVSTIEPENIKALLATQFNDFGLGTRHREFYPLLGDGIFTLDGPGWSHARGL

LRPQFTRDQVADLELMDGHISRMIDLIPQDGSSFDIQRLFFLLTIDSATHFLFGESVGALESSNSASLLG

RSSVGSAQGFAEAFGTAQDYLTTRSRAMHFYWMVNPKEFREANQRVHEVVDHYVQLAIQSKNNPDKKSDR

YIFAEALAADNDDPKVLRDNMLNILVAGRDTTASLLSSAFFYLSRNQDVWKKLRQTIIDEFGDSQNPKGE

ITQAKLKDIPYLRYVLNEVLRLLPPVPLNFRVAAKDTSLPVGGGPDGRSPVFIPKGQVVAYSVYAMHRRT

DLYGPDSHSFRPERWEENGRRGWDYLPFNGGPRICLGQQYALTEASYTLVKLVQRFDTLECADPELKQPA

ILSTLTMSHDRGVKVRLSSSVPKCWFNQ

#16 44% to 52A5, 44% TO CYP52J1

>CYP52K1 BAE66393.1 [Aspergillus oryzae]

MLTLSFSALLGIALAWAIHRLAWKYSRLKAICVKRDNKLATQYGCEQPPRLRNWWPLGIDRLIQIWTADS

EQRLMDLFTFPFKDVGYTLEQKFLGTIAFGTIDPENLEAIMTNINVFSFGLRRHILFPLLGDGIFTQEGK

SWRHSRELLRPQFTRQHYRDLNIFRPHVDRLLHYLTGNGAATDLQPLFFRLTLDTTTEYLFGKSVNSLMP

GEATKGQSFANHFDMAQNYVVQRFRLLDLYWLIGGPKFWRSCSAVHRFIDEIIDTRMDSKEKDKDCNSKS

IFFDAVARDSRTRRDLRDQLTNVLLAGRDTTACLLSWTFHCLARHPDVLGRLNVEIASVVGTNTDLTRDD

FKRMPYLDQVLREVLRLYPPVPVNTRTAHKTTILPTGGGKDGTKPFMVREGENVAFCVYAMHRREDLYGP

DAGQFRPERWDEDLPLFQNERTATWGYLPFNGGPRACLGQDFGFVEAAYTVVRILQKYPIVKPDMYKGDI

QGRKWLGWSSHQPEGIEMVTEERQKMTIVLSLGDGCRVTLGR

#83 58% TO SEQ 84, 81% to 53A3

>CYP53A13 BAE60506.1 [Aspergillus oryzae]

MIAELLTPTGAAYVLTAAVIVYYILPYLQLWRLRDIPSPGFAAFSNLWLMLQYRKGNRFVTVDNAHKKYG

KLVRIAPRHVSIADDEAIQAIYGHGNGFLKADFYDAFVSIRRGLFNTRDRAEHTRKRKTVSHTFSMKSIG

QFEQYIHGNAELFVKQWNRIADTQSNPKTGYATIDALNWFNYLAFDIIGDLAFGAPFGMLEKGQDIAEMR

KSPNDKPSYVQAVEVLNRRGEVSATLGACPSLIPWAKYIPDRFFRDGLEAVENLAGIAVARVNERLRPEV

MANNTRVDLLARLMEGKDSNGNKLGREELTAEALTQLIAGSDTTSNTSCAILYWCLRTPGVIEKLHKVLD

ESIPKDVDVPVHAMVKDIPYLQWVIWETMRIHSTSAMGLPREIPAGNPPVTISGHTFYPGDVVSVPTYTI

HRSKEIWGPDAEQFVPERWDPKRLTARQKAAFIPFSTGPRACVGRNVAEMELLVIVGTVFRLFDFEIQQD

GPMETREGFLRKPLGLMVGMKRRSVAV

#84 56% to 53A3

>CYP53A14 BAE56637.1 [Aspergillus oryzae]

MDGDSCPSCCSTCTSLNVFLCPHIPILTLGLLLVYYVTGYLKRWHLRDIPGPFIAGFSRIWLIVQVRQGY

RSLVVHDLHRRYGKIVRLAPNHISIADESAIQAIYGHGNGFLETDFYNAFLNVDWSIFTTRSRAEHTRKR

KIVSHAFSARSLAQVEQHAHNNMEHLVRQWRKMIDSEEGLDDPYAVIDARVWCNYLTFDIIGDLAFGAPF

GMLERENATVSMRKAPENPEVTLDAVEVLNHRGDVSAAFGICPDLIPYAKWLPDPFFRQGAEAIANLAGV

AGAAVDRRLKMDTSMTEKRGDLLALLIDAEDQAGAKLGHRELTGEAVTLIAAGSDTSSSTLCALLYWVSS

TPRVLWKLQNVLDEVIPVDIEVPYLAMVKKITYLQWVIWEALRIHSTFGQGLPREVPPERGPVEICGHTF

YPGDVLSVPGYTMHHSADIWGIDVEDFVPERWDPRRLTQRQKDSFIPFSEGPRACIGRNLAEMELFVGCA

TLFRLFEFRVEGQGPLKVRERWLRKPVSLQVGIRRRYLDARSS

#45

>CYP55A5v1 BAC01275.1 cytochrome P450nor [Aspergillus oryzae]

MNSEPVYPRFPFARPSGDEPPAEFHRLLRECPVSRVELWDGSHPWLVVKHKDVCEVLTDPRLSKVRQRDG

FPEMSPGGKAAARNRPTFVDMDAPDHMHQRSMVSAFFNDEYVESRLPFIRDTVQHYLDRLIRAGKDGKEV

DLVKHFALPIPSHIIYDILGIPIEDFEYLSGCDATRTNGSSTAAAAQAANKEILEYLERLVDKKTTNPSH

DVISTLVIQQLKPGHIEKLDVVQIAFLLLVAGNATVVSMIALGVVTLLEHPDQLSRLLEDPSLSNLFVEE

LCRFHTASALATRRVATVDIELRGQKIRAGEGIIASNQAANRDPEVFPDPDTFDMFRKRGPEEALGFGYG

DHRCIAEMLARAELETVFSTLFQTLPSLKLAIPKSEIQWTPPTRDVGIVGLPVTWDRD

#45b allele?

>CYP55A5v2 BAE61721.1 [Aspergillus oryzae] 6 aa diffs to v1

MQLEPVYPRFPFARPSGDEPPAEFHRLLRECPVSRVELWDGSHPWLVVKHKDVCEVLTDPRLSKVRQRDG

FPEMSPGGKAAARNRPTFVDMDAPDHMHQRSMVSAFFNDAYVESRLPFIRDTVQYYLDRLIRAGEDGKEV

DLVKHFALPIPSHIIYDILGIPIEDFEYLSGCDATRTNGSSTAAAAQAANKEILEYLERLVDKKTTNPSH

DVISTLVIQQLKPGHIEKLDVVQIAFLLLVAGNATVVSMIALGVVTLLEHPDQLSRLLEDPSLSNLFVEE

LCRFHTASALATRRVATVDIELRGQKIRAGEGIIASNQAANRDPEVFPDPDTFDMFRKRGPEEALGFGYG

DHRCIAEMLARAELETVFSTLFQTLPSLKLAVPKSEIQWTPPTRDVGIVGLPVTWDRD

#46

>CYP56C1 Aspergillus oryzae

GenEMBL AP007164.1c

third P450 of six on this accession

CDS join(1160825..1161472,1161531..1162415,1162505..1162558)

47% to CYP56B1 Aspergillus nidulans

41% to CYP56A1 S. cerevisiae

MVMHGLCIIIGKALAVGILFLSICIFSRIVPPRYPRNIPAVPFW

VTLLSLVRDIDQEDIYRRHIQKPLQTHGAIKIFFAGQWNLLIQRSSYLNEIFRNEDVY

QKSGNQKKIPHSVLAEFL (1?)

GDNVISSRGTTWRLYRDIITPG

LQGHFDAGLIAANAEELCSSLLAFQNTVGNCGVPVQDLLQQFTIANVSQVLLQANHKP

TKGDESSLHQLQLAVKREIFKPIFMNFPVLDRLGKLIPCRVRARNVVEQFSAALQYGV

RHGQGTPNASNLGARLMAARDGGVLTGKQFRDNLNVLFVAGQENPQLLLISMLYLLAKHP (0)

DVQSRLRREIDACSTLDPSNVAFSELPYVTCVIYESLRLLP

PISQLINRRTSQDVILGNQIYIPKGTYVGYNCYSTNRDPAVWGPTADEFRPERWGQSN

TEISQCYRQRRARAEFVSFHGGSRACLGEKFALLEARVALFVLVSRLSWSLDPEWPDR

KTPAGPLYPRALRLIFTERK

>CYP56C1 BAE62147.1 [Aspergillus oryzae] assembly errors

MVMHGLCIIIGKALAVGILFLSICIFSRIVPPRYPRNIPAVPFWVTLLSLVRDIDQEDIYRRHIQKPLQT

HGAIKIFFAGQWNLLIQRSSYLNEIFRNEDVYQKSGNQKKIPHSVLAEFLGRFPVSPSVALLLTLGPKGD

NVISSRGTTWRLYRDIITPGLQGHFDAGLIAANAEELCSSLLAFQNTVGNCGVPVQDLLQQFTIANVSQV

LLQANHKPTKGDESSLHQLQLAVKREIFKPIFMNFPVLDRLGKLIPCRVRARNVVEQFSAALQYGVRHGQ

GTPNASNLGARLMAARDGGVLTGKQFRDNLNVLFVAGQENPQLLLISMLYLLAKHPVSVSCPLKSMELMS

QDVQSRLRREIDACSTLDPSNVAFSELPYVTCVIYESLRLLPPISQLINRRTSQDVILGNQIYIPKGTYV

GYNCYSTNRDPAVWGPTADEFRPERWGQSNTEISQCYRQRRARAEFVSFHGGSRACLGEKFALLEARVAL

FVLVSRLSWSLDPEWPDRKTPAGPLYPRALRLIFTERK

#102 56% to 57B2, 55% to 57B1

>CYP57B3 BAE55311.1 [Aspergillus oryzae]

MIGTVLDTVLGNPQGAVGGLFVGSFVIFWVVPFLYNLFFSPLRNVPGPFWARFTILWEFSQLMKGRSHEE

YIKLHKKYGPVVRVSPKRYSVIDPQDVKKIYGFGADFPKSEFYDSLGDPKNIFTVRDNEDHKDRRRKVAS

LYTMSSMVAYEDAVDRMTTLCIKKMTDLAASRKLISIPKFMQFYAFDVIGEITFDQNFGMMENMGDTQGI

IKEIHAMNNTIGIMGLLPELQSTWRAAQKVLFSGTAMGKLTEYIWSQYWKHRNTNSGAKQKSQYDTFLRK

VLELEAAHKVGRMNVLDSCGSNIGAGSDTTGITLSAALWYIYRNPDKLAKLRHEIDTMTAEGRISDPVTY

KQAQEMPYLNAVIKETLRVHPGVGTILARVVPKGGITLTGGYYVPEGTHIGTNAWPLHYSETVYGPDADQ

YRPERWLEDKPQPDYRDSMMFAFGSGSRTCIGRNISLLEITKVLPQIVRKFDLKFEEKKDPWDAWCAWFV

YPKYKCWIEPRKPVENVA

#47

>CYP58D2 BAE58658.1 [Aspergillus oryzae]

MGVISVVTFLASAKEHFIISILLLFPIALILRTIYRLYIDPLHHIPGPKLAAISHLYEFYHDVVRGGLFI

WEIEKMHQEYGPIVRINPREVHIKDPYFYDELYAPAHGWRDKDAKSVEIFSSPNALVSTVDHHTHRMRRK

LLTSFFSRRSIERIEPVIHESLSKFLDSLITAYEEDSVVELIDRLQALTGHVITQYAYGEDYGLHEPQNI

GKGIVKVVQEGTEQIHLHRFFPLIQRFLRLIPSFFMTQLFPARAAMYDLLHGVRKKSIEVLQQKDVCTPT

ERTTMFHALTAPEVPPEERTLQRLEDEGLVLFAAGTETTATTLGVAIFHILSDPMVLTKLRKELEQVMPT

PEGLATWRELEKLPYLNGVIHEALRFSGLAMRQQRVSPTEVIKYKDYAIPPGTPVSMLQYFLHTDPALFS

DPEKFYPERWMLAAERKESLSRFLVTFGKGTRSCIGMNLAYAELHTALAAIVRRFDLELYETTAEDIRFV

RDKLLPRAKNGPWRVRVKVVGIRKD

#90 61% TO 58E1 47% TO 58A2, 41% to 58C1, 46% TO 58A3

>CYP58E2 BAE59845.1 [Aspergillus oryzae]

MLDTISESRPNGLATLGVVVATFATYLALKALYNLYLHPLRKFPGPKLAAIGPYYEFYYDVMKDGMFLWE

MERMHQVYGPIVRVNANEIHIRDPHYYSTVYAGNHRSTDKYHDAVAAFSVPQASLATIHHKVHRLRRSIL

NPYFSKAAVTRLESAINERIERMCSRLEETMHYGQVVDLDAGFAALTADIVTTYFYGQNFDYLGNEGFKF

QVRDAILGLIQFYHFTRFFPWIANTIKKLPIPIMRLIHPGAAYLVSSQEEIKDSIRASLDKGNKADAKSV

IVQALEDPTIPPQERTLDRLGDEGTTIIFAGTETTARALSVGMFHILNNKTILKKLREELDTLPGVSSGV

YSHVQLECLPYLTGAVQESLRLSHGPAIRLPRVANDKALKYGDYIIPPGTPVSLCTVLVHQDPCIFPDPH

RFDPERWVKASKEGVNLDKFIAAFTKGTRQCLGINLAYAEIYLTFAKLIRTFNMEIYDTTVDDLTVHHIR

LTGAPKQGTGEVKVKVTEKIL

#125 46% TO 58E2, 40% to 58C1, 45% to 58D1, 44% to 58B1

46% to 58A3

>CYP58F1 BAE64028.1 [Aspergillus oryzae]

MALLVILGIGFVTYLVFLSIYRLYFHPLSHIPGPKLAAITHGYEFYHNIIRGGLFIWELERLHEVYGPII

RINPREVHIKDPDYYDDIYASSARKREKDPVLVAQFGLEGSGFSAIDQETHHQRRAPVAKFFSKRAIENQ

EHLIRDSLNKLVGHFQEACRAHTVVSLDAAFAALTSDVIHQYAYGFNPNNLDKEGFNAKVRDGINGLFQL

AHLLYFFPILQTLMNVMPLPLLQVLNPPAFALASQKKELYEQGAAALEKANSKAGSGTLIDTLAAPSMPE

HMRSAHRLMNEGFALVIGGTETTARSLALGAWHLFTREDIRNKLREELKQVMPTPDSQPTWNDLEKLPYM

SGVISESLRLSTGIANRSSRVAPTEALVYKDYTIPPGTPVSETNYFTLMDPEIFSDPHAFDPERWIRAAA

KGQRLDRYLVNFSKGSRMCVGLNLAYAELFLVIATIVRRFDIELYETPKSNIDFARDFGTPYPDKGNFSV

RALVTGVISE

#126 40% to 58C1, 41% to 58D1, 39% to 58B1, 35% to 58A3

39% to 52E2, 41% to 58F1

>CYP58G1 BAE64022.1 [Aspergillus oryzae]

MNLPLIWIGLFTAVSYLVIRSIYRLYFHPLSNFPGPKLAAVTHLYEFYYDVVKGGKFIWEMQRMHDQYGM

YCTTIHIKDPYYFDPIYTSKGQAKDPYIVRTFATPLSTAATVEHDRHRYRRDLVNPFFSKRSVMGVDYIV

QDKVDKVCKRLTQVHERGTVVSLDDLFAALTADVISHYAYGESLGFLDTENLKNEFRDAVASAGLLCHFA

RFFFVVSMVAETMPALVEWMQPSSKGLWEAKRMIEQMARSSLEKDHEKNANSRKTIFDALCAESVRPEER

TVARVRDEAMVVFGAGTETTARVLATGSYYLYRDKPRLEKLRAEIETVMPDSTDHVSLTQLESLPYLTAV

INESLRMAHSVTMRLPRISPTPLAYKDYIIPPGVSHLDAMELLRSNTSRSDTDSFNPERWLEASSKGERL

TKFLVPFSKGSRICLGMNLAYSELYQMFATLVRCFDLEIQTPPESVRITRDFIIGLPDDADYLKVHSLVT

NAL

#153 43% to 58B1, 44% to 58C1, 42% to 58D1, 38% to 58A3

42% to 58G1, 40% to 58E2, 42% to 58F1

>CYP58H1 BAE56719.1 [Aspergillus oryzae]

MDEFTISAGSLCLLSLVVGFIIQSVYRLFFHPLRKFPGPKLGAISHLVQGLCFANNPKGPVVRINPRELH

INDPYYYEQIYAGSSRIREKDPRFIGVFTTPLPMVATVGHEHHRIRRGLLSSYFSRRALKKAELIIDQKV

DRLLVRFHSAFKCHAVLPLQRVFAALAADIVSEYCYGASQGYLEQKVFQNQMIDAVNYVMSMCHINKSIP

IIPKLLRCVPVGLMEKLGLQMADVIGVRNLIRRQAAKSLDKEWLSHDTNMLSKNVFDAIAAADVAPQEKT

LRRLEEEGAALFGAGIETTARALTVAMFHLISDETMIRKLRDELKQVMLSPASRPTWAELEQLPYLTGVV

NESLRLSFGLVARSPRVSPIESLAYGEYVIPPG (0)

TPVSQSAYFVHMNPQV

FPEPESFNPERWIKAAEKGQYLSRFLVAFSKGSRQCLGMNLAYAELYLTLARIVRLVDMKLVGTTIDNIR

VGRDLGHPAPKAGNFKVKVEVMGIASKS

#136 41% to 58C1 46% to 58C1, 44% to 58D1, 41% to 58B1,

40% to 58A3, 43% to 58E2, 42% to 58F1, 41% to 58G1,

41% to 58H1

>CYP58J1 BAE62179.1 [Aspergillus oryzae]

MDSPRLWTGLAGLITYLIVISVYRLFSHPLRNIPGPKLAAVTHLYEWYYDLFLGGKYLFEIERMHERYGP

IVRINPPEIHINDPKYYDEIYASGTHRRNKDAEFVSFTGLLLSSASTTDHDLHRYRRGLMNNFFSKKSVR

GISYFVEEKVHNLMQRFEAFYRCNKVVRLDDAFAAMTSDVITHYCYGKSWDYLDYANLRTDVRKAVRDLT

CSVHFNRIFPIFLAVLKKLPLRWLYAIHPGRSVVLDIQKTIYEQSAEAIHGDKYKIGHNDAVDKHKTIYD

QLTDPSIPAEERSLQRLQDEGLLLISVGTETTARALTTACFHIASDDQLRTRLREELRTVLPTPTSSVTW

SELEKLPYL (0)

TGTVNESLRLGGFLTTRSPRIAPDEPLTYKEYTIPPS (0)

TPVSSSSYFGHKNPNIFPEPEKFSPERWISAGRNNDHLFKYITSFSRGSRICAGMNLAF

LELYMTLAYFVRRFDVELVDTTVEDMKIVRDMRVGFTHRGEPTVYGRIVRVYED

#134

41% to 58J1, 38% to 58A1, 40% to 58D2, 39% to 58C1,

38% to 58B1, 42% to 58J1, 37% to 58H1, 37% to 58G1,

39% to 58F1, 39% to 58E2

>CYP58K1 BAE63021.1 [Aspergillus oryzae]

MVMDQAWTWMALVAIAGLFWITRIFYRLYFHPLAKIPGPKLAAASHLLEFYYDVILGGKFLFQVEKMHQK

YGPIVRINPKEVHIIDPTFYNEIYASGMRKRDKYEGFVRSLAADESTVSTVGSEKHRFRRSILQNFFSKR

SVMEFSSAIGERVEKLMRRLEVFEKTQTPVALDVVFSALTSDMITYICYGKDWKFLDHKDFNCDIHQAGV

DFANFFHFNRFFPWVFMTLRALSPRMLALLIPGRAATFKFQESLLKHAIEMAANEQSDAPSKETEKSRPN

VISNLINPSIPYMERSRRRLEDEVITILVAGTEAPVKVLSMAMYYLGSEPAIGEKLRAELKTILPARTST

ATYAELEKLPYLHGVVYESLRISDSVIARFPRIAPTETLRYKDHILPPGTPMSCSSYFISRNHDLFPNPE

KFDPERWIHAAEKGENLKQHLTAFTKGSRICLGINLTIAELFLTIAHMCRRYHILLHNTEPEDVCTTSDL

LAGYTRRGVLKVHAKLKAVRE

#5 72% to 59A1

>CYP59A4 BAE59520.1 [Aspergillus oryzae]

MPEFQLLAGHFGTLKKTIQGMPSDATLHSIMLKISQQFPSGIFYINMWPFSGTWMIVSTPSAASQIQKLN

LSKPAILRRPLEMVTGGPSMMSMHGETWKKWRALFNPGFNPAYIIGLAPNISDEVAIFCAQLRKIAQQGE

VFPLESLTTRLTVDSICSVVLDTQLHHQIKDHPLATALQRQIDWTSFGTTFNPFKRYLTIRPLVLWYNNK

FMDRIIDGEVDRAYCTPPGHPSKSVISLALREYLQEQASNNSTRSLAEFKRLVAPQLRVFLFAGRNTTSS

TLIYTYYLLAQHPEALAKIRAEHGDVLGADPAEAQGRIKEDVQLLNKLPYTTAVIKETLRLFPPSASMRE

GRPDAEIIGEDGQRYPTVGCNVWTLTVALHHNSDYWDQVENFIPERWLVGPEDPLYPVKGAWRAFEFGPR

SCIGQTLAMLELRIALAMTIRQFDITPAYDEWDSIHPATTAREVNGHRAYQAERGAGGAHPADGFPCRVK

ERC

>BAE71324.1 monooxygenase [Aspergillus oryzae]

MPEFQLLAGHFGTLKKTIQGMPSDATLHSIMLKISQQFPSGIFYINMWPFSGTWMIVSTPSAASQIQKLN

LSKPAILRRPLEMVTGGPSMMSMHGETWKKWRALFNPGFNPAYIIGLAPNISDEVAIFCAQLRKIAQQGE

VFPLESLTTRLTVDSICSVVLDTQLHHQIKDHPLATALQRQIDWTSFGTTFNPFKRYLTIRPLVLWYNNK

FMDRIIDGEVDRAYCTPPGHPSKSVISLALREYLQEQASNNSTRSLAEFKRLVAPQLRVFLFAGRNTTSS

TLIYTYYLLAQHPEALAKIRAEHGDVLGADPAEAQGRIKEDVQLLNKLPYTTAVIKETLRLFPPSASMRE

GRPDAEIIGEDGQRYPTVGCNVWTLTVALHHNSDYWDQVENFIPERWLVGPEDPLYPVKGAWRAFEFGPR

SCIGQTLAMLELRIALAMTIRQFDITPAYDEWDSIHPATTAREVNGHRAYQAERGAGGAHPADGFPCRVK

ERC

#6 92% to seq 5, 71% to 59A1

>CYP59A5 BAE48804.1 monooxygenase [Aspergillus oryzae]

MPEFKLLAGHFGTLKKTIQGMPSDATLHSIMLKISKQFQSGIFYINMWPFSGTWMIVSTPSAATQIQKLN

LTKPAILRQPPETVTGGPSMMTMHGETWKKWRALFNPGFNPAYIIGLAPNITDEVATFCAQLRKKAQQGE

VFPLESLTTRLTVNSICSVVLDTQLHHQIKDYPLATALQRQINWTSFGTTFNPLKRYFTIRPLVLWYNNK

VMDRIIGGEVDRAYRTPPDHPSKSVISLALREYLQEQASSNSTRSLAEFKRLVAPQLRVFLFAGRNTTSS

TLIYTYYLLAQHPDILAKIRAEHEDVLGVNPEEVQGRIKEDAQLLNKLPYTTAVIKETLRLFPPSASMRE

GRPDAEIIGENGQRYPTVGCNVWTLTVALHHNSDHWNQVESFTPERWLVGPEDPLYPVKGAWRAFEFGPR

SCIGQTLAMLELRIALAMTIRQFDITPAYDEWDSIHPATTSKEVNGHRAYQAERGAGGAHPADGFPCRVK

ERF

#112 55% TO SEQ 97, 41% to 59A1, 41% to 59C1

>CYP59D1 BAE66084.1 [Aspergillus oryzae]

AP007175.1

470087 MVIAVSDLVGSYGARVALVLALVIVLRFLQEMLKVRLLFYRLRKQ

GLPMPKWNFAAGNLQMLPDLMKRHP

KGSQQSEAFTLLSYEFASSDNCFYIDVWPFTKPLLVVNSPDLAVQACQTYALPKPPVLAKFFNPFAGGPS

IFTTNGPEWKRNRGLFNPAFSTSNILQHTPHI 470671

VEEAEEYVEILREHARKGDTFTLDKMTCDYVLDIIGRVAM (2)

KARLHSQRGRNPVAAALRSQVEWHCQDEQMNPFIRWNPMRPIMQWCNGRTM

NQYIGAELDKRYEAWTQNKPSTRANSIIDIVLAEYMSTRPVRAALDPEFKSWAT

IQLRTFLFAGHDSTAATIVYSIYLLSKHPEILSKVRTEHDEVFGSDISAAAGILKQHPEL

INRLPYTLAVIKETLRLFPAASALREGQPGVYLQDKNGTKYPTEGLCIWIIHGGIQRNPN

YWPDPHAFKPERWLVGPDHPLYPPKGAWRPFEQGLRDCIGQALALLDVKITLVLTLREFD

FQDQYAHWDRLHPRSGPKTVFGERAYQIPQGGSHPVDGLPCRVSLRNQITK*

#97 37% to 59A1, 37% to 59C1

>CYP59D2 BAE58153.1 [Aspergillus oryzae] AP007155.1

2825205 MGANTLGWDGLTSRIAVAVATVCLTSFVYKLIKMRLMFYRLRKKGL (0) 2825068

2825008 PTPPWNPILGNLAVMAQLQKKWPSDSREAESFALLSTEAPGCEAGFYVDVWPFSIP

MLVVTSPALAVQACQTYDLPKPDVLQPFINPMAGGSDNLFVSNGAHWKQARELFNHGFSMAAAMSHMTYI

LEEAQVFVQMLKDHARKGDTFSLDALTCRYVMDIIGNVA LNTRFRFQEQHNPIAAAMRDTIELECGIETS

NFLSRWNPRRLYRQWQNGRTMDYLIGVELDKRYKEWRETAKSSSHPRTQSIMDMVIAEYMKTRPQAQQQQ

ELDPEFKRWATIQIRLFLFVGHDSEATTIIYSLYLLSKNPGVLIKVRAEHDRVFGAGVSSAYDVLTDHPE

KINQLSYTHAVIKETLRLFPPANGLRGGLPGVSLRDEQGRIFPTEGCAIWIVHTAVHRNPSSWPQPHAFI

PDRWLVEPGHPLYPPAGGWRPFEQGPRNCIGQNISLLGIKASLAMLVRQFDFHDAYAEYDRLHPSTGLKT

MFGERAYMIQKGAGHPAQGFPCKVTLR

#2 95% to CYP60A3

>CYP60A5 BAE71325.1 cytochrome P450 monooxygenase [Aspergillus oryzae]

MGGDGWPSDGHILLLIVLTVLTPPSLALYRLWIHPLRSYPGPRWWAIWRGPYILSNIRGNLVRDLQRLHQ

QFGHVVRIAPNELSFIVPEAASPIYTSNPEFPKDPMHLPPFHNGTPGILAADHAHHRRYRRLLAFSFSDK

GLRQQRSLIERSVNLLITRLHENCGQGPLDLTLWFNWATFDIIGDLAFGNSFGCLDNVQTHPWISSIQGN

VKLIPILNAFRRYRLDGLLQLLGSRKLLEQRRRNAQFTTDQVDRRLKNSSTPRGDIWDAVLAQKPDGEPP

MSREEMISNTSAIVLAGSETSATLLSGCTWLLLKNPGHLHQLTSRIRSQFTHASEIDSQSVSRVEGLQAI

LEESLRLYPPVPMQSNRIVPQSGAYIAGGWVPGGTSVGLQQFVACRSSSNFHRPEEFLPERWQGQGEFAH

DRREVSQPFSIGPRNCIGRQLAYVETRLILVKLLWHFDLRLDTTRMKDTDWLAEQGIWILWDKNPLWVNL

EPRNE

>BAC45242.1 cytochrome P450 monooxygenase [Aspergillus oryzae]

MGGDGWPSDGHILLLIVLTVLTPPSLALYRLWIHPLRSYPGPRWWAIWRGPYILSNIRGNLVRDLQRLHQ

QFGHVVRIAPNELSFIVPEAASPIYTSNPEFPKDPMHLPPFHNGTPGILAADHAHHRRYRRLLAFSFSDK

GLRQQRSLIERSVNLLITRLHENCGQGPLDLTLWFNWATFDIIGDLAFGNSFGCLDNVQTHPWISSIQGN

VKLIPILNAFRRYRLDGLLQLLGSRKLLEQRRRNAQFTTDQVDRRLKNSSTPRGDIWDAVLAQKPDGEPP

MSREEMISNTSAIVLAGSETSATLLSGCTWLLLKNPGHLHQLTSRIRSQFTHASEIDSQSVSRVEGLQAI

LEESLRLYPPVPMQSNRIVPQSGAYIAGGWVPGGTSVGLQQFVACRSSSNFHRPEEFLPERWQGQGEFAH

DRREVSQPFSIGPRNCIGRQLAYVETRLILVKLLWHFDLRLDTTRMKDTDWLAEQGIWILWDKNPLWVNL

EPRNE

>BAE59521.1 [Aspergillus oryzae]

MGGDGWPSDGHILLLIVLTVLTPPSLALYRLWIHPLRSYPGPRWWAIWRGPYILSNIRGNLVRDLQRLHQ

QFGHVVRIAPNELSFIVPEAASPIYTSNPEFPKDPMHLPPFHNGTPGILAADHAHHRRYRRLLAFSFSDK

GLRQQRSLIERSVNLLITRLHENCGQGPLDLTLWFNWATFDIIGDLAFGNSFGCLDNVQTHPWISSIQGN

VKLIPILNAFRRYRLDGLLQLLGSRKLLEQRRRNAQFTTDQVDRRLKNSSTPRGDIWDAVLAQKPDGEPP

MSREEMISNTSAIVLAGSETSATLLSGCTWLLLKNPGHLHQLTSRIRSQFTHASEIDSQSVSRVEGLQAI

LEESLRLYPPVPMQSNRIVPQSGAYIAGGWVPGGTSVGLQQFVACRSSSNFHRPEEFLPERWQGQGEFAH

DRREVSQPFSIGPRNCIGRQLAYVETRLILVKLLWHFDLRLDTTRMKDTDWLAEQGIWILWDKNPLWVNL

EPRNE

#1

>CYP60B3 BAE71326.1 desaturase [Aspergillus oryzae] 81% TO 60B1

MYFLSLPALAIIVPVGYVLLHLGYNLFFHPLRGYPGPLLWRASSLPWKIALLRGTMHHDLMRFHQKYGDT

VRVKPDEISYANAQAWRDIHAHVPGRPEFLKDPVRLPLAPNGVMSILVSDTKNHARFRSLFGHAFSDKGL

RTQESTIVQYADLLVEVLREMANTGRSAEMVYYFNMAIFDSIGALSFGESFDSLKSRQLHPWVDAIHKNL

KSVAISHVLRSMGIEFLTPYVLPKELRGKRQENYSYAVEKLNKRMKMEGDLGDFWDKVLVKSADDNQRGD

GMSAGEMLNNAAVMVVAGSETTASALSGAMYLLCLSGKIEKATAEIRKSFASPEDIDLISVSHLPYLAAV

IDETLRMYPAVPGQPPRVVPAGGATVCGRFVPEETRVGVSHLGAYFADYNFTHADKFIPERHLQKTEEPY

KYDNYGAYQPWSVGLRNCIGRNLAYAEVRLTLAKLLWHFDFTLDVGKTGNFLDQKIWSIWAKRELYMFIK

TRGTSSSSPQ

>BAC45243.1 cytochrome P450 monooxygenase [Aspergillus oryzae]

MYFLSLPALAIIVPVGYVLLHLGYNLFFHPLRGYPGPLLWRASSLPWKIALLRGTMHHDLMRFHQKYGDT

VRVKPDEISYANAQAWRDIHAHVPGRPEFLKDPVRLPLAPNGVMSILVSDTKNHARFRSLFGHAFSDKGL

RTQESTIVQYADLLVEVLREMANTGRSAEMVYYFNMAIFDSIGALSFGESFDSLKSRQLHPWVDAIHKNL

KSVAISHVLRSMGIEFLTPYVLPKELRGKRQENYSYAVEKLNKRMKMEGDLGDFWDKVLVKSADDNQRGD

GMSAGEMLNNAAVMVVAGSETTASALSGAMYLLCLSGKIEKATAEIRKSFASPEDIDLISVSHLPYLAAV

IDETLRMYPAVPGQPPRVVPAGGATVCGRFVPEETRVGVSHLGAYFADYNFTHADKFIPERHLQKTEEPY

KYDNYGAYQPWSVGLRNCIGRNLAYAEVRLTLAKLLWHFDFTLDVGKTGNFLDQKIWSIWAKRELYMFIK

TRGTSSSSPQ

>BAE59522.1 [Aspergillus oryzae]

MYFLSLPALAIIVPVGYVLLHLGYNLFFHPLRGYPGPLLWRASSLPWKIALLRGTMHHDLMRFHQKYGDT

VRVKPDEISYANAQAWRDIHAHVPGRPEFLKDPVRLPLAPNGVMSILVSDTKNHARFRSLFGHAFSDKGL

RTQESTIVQYADLLVEVLREMANTGRSAEMVYYFNMAIFDSIGALSFGESFDSLKSRQLHPWVDAIHKNL

KSVAISHVLRSMGIEFLTPYVLPKELRGKRQENYSYAVEKLNKRMKMEGDLGDFWDKVLVKSADDNQRGD

GMSAGEMLNNAAVMVVAGSETTASALSGAMYLLCLSGKIEKATAEIRKSFASPEDIDLISVSHLPYLAAV

IDETLRMYPAVPGQPPRVVPAGGATVCGRFVPEETRVGVSHLGAYFADYNFTHADKFIPERHLQKTEEPY

KYDNYGAYQPWSVGLRNCIGRNLAYAEVRLTLAKLLWHFDFTLDVGKTGNFLDQKIWSIWAKRELYMFIK

TRGTSSSSPQ

#48

>CYP61A1 BAE58068.1 [Aspergillus oryzae]

MANVTGSFVSPSADATVVPQLFQPSGLLGSLLGDFNVWKGLLTLFIAAVIYDQFRYFYQKGSIVGPRWKL

PFMGPFLQSVNPKFHEYKAKWDSGELSCVSVFHKFVVIASTRDMSRKIFNSPAYVKPCVVDSAHKLLGED

NWVFLDGKDHVEFRKGLNGLFTRSALSCYLPRQEETFNQYFKHFLEKSKANGYKPTPWMPEFRELMTAIS

CRTFVGHYMTDEVIQKINDDYYLITAALELVNFPIILPFTKTWYGKKAADMVMEEFAKCAAKSKARMAAG

GEVSCIMDAWVKAQQVSAKYREDVAKGIPAEKPPQLLRDFTDEEIAKTVFTFLFASQDATSAASTWLFQL

MADRPEVLEKVREENVRLRNGDINAPITMELLDQMEYTRAVVKETLRYRPPVIMVPYLVKKDFPITEKIT

VLKGSMIIPSVWPATHDEEAYPNPDTFDPDRWITGTAEQNPKNWLVFGTGPHYCLGQTYAQLNLMAMIGK

ASMEMTWEHTTTPKSEDIKVFATIFPQDDCLLTFRPRA

#49

>CYP61A6 BAE57461.1 [Aspergillus oryzae]

MDAKMATEIFNTTSAQPESPQSWAYKTSQLQGALPDFLAEWSAWQYVVTFLVGLVLYDQVLYLKRKGALP

GPTFKIPLMGPFIQAIHPTFDGYLRQWASGPLSCVSIFHRFVVLASDRDLAHKVFKSPAYAEPCLVPVAR

DIIGHKAWVFLQGRDHAEYRRGLVPLFTSRAIATYLPVQERVLDDYYNQFVAATKANQGKPMAFMTLFRE

INCALSCRTFFGDYISDDQVKKIADDFYLATDALDLVNIPLAMYVPFTKTWLGKRTADAVHRVFAECAAR

CKANMAKGATPTCIVDHWVLHMMESNRYRERVAAGETNLEKPKNMIREFTNEEISDTLFTFLFASQDASS

SATTWLFQILAQRPDVLDKLREENLAARGGDRNKPFDLPMLESLTYTNAVIKELLRYKPPVILVPYLATK

DFPVTPDYTVPKGSMIIPSCYPALHDPEAYPNPDVFDPERWISGDAESKTKNWLVFGAGPHDCLARKYVP

LSLAGMIGKASLELDWEHHPTPRSEEIRVFATLFPMDGCNLVFKRRP

#3 66% to CYP62A1 An ortholog

>CYP62A1 BAE71332.1 cytochrome P450 monooxygenase [Aspergillus oryzae]

MTNTASRELIRAIEHVPLTWWFLAVGGAWIVSKIVKILQTAYFSPLRKVPGPWYARLTSARLAWASFANN

RIYYVQSLHEKYGPIVLIGPEEVDIADPVAAKQIHRMGSGFVKAPFYKLLSPGPVDNIFNFRDAKLHSTR

RKLYAKGFTLNSLRQQWEPTIRNIVALTVERIRHDAQQGEAEILGWWTLMANETVCKLTFNGGHDTVRNG

TKDPFVLMLERRMGDLAHLLQHFAPPLYYLGRLLGRAEPRLHDVFFSQETMFEAGKHVVAIARSARDAEG

DRNLFVKALAAGDLESKIGCLNDTEIITDAGALLLAGSDPTALSLTYLIWCVLNRPKLQADLESEVAGLQ

GDITDAACADLPTLNAVICESLRLYGPAPGSMPRSPPPDGATLCGYYIPPSAVVVTQNWSLHGNPKVWKD

PHTFDHTRWLPGSSLSEEAKMSFNPFGQGARQCLGIHLGWMQLRLATALFFRRCPGAKLAPSTTPESMVM

IDSFIAGMPKARRCAIQL

>BAE59529.1 [Aspergillus oryzae]

MTNTASRELIRAIEHVPLTWWFLAVGGAWIVSKIVKILQTAYFSPLRKVPGPWYARLTSARLAWASFANN

RIYYVQSLHEKYGPIVLIGPEEVDIADPVAAKQIHRMGSGFVKAPFYKLLSPGPVDNIFNFRDAKLHSTR

RKLYAKGFTLNSLRQQWEPTIRNIVALTVERIRHDAQQGEAEILGWWTLMANETVCKLTFNGGHDTVRNG

TKDPFVLMLERRMGDLAHLLQHFAPPLYYLGRLLGRAEPRLHDVFFSQETMFEAGKHVVAIARSARDAEG

DRNLFVKALAAGDLESKIGCLNDTEIITDAGALLLAGSDPTALSLTYLIWCVLNRPKLQADLESEVAGLQ

GDITDAACADLPTLNAVICESLRLYGPAPGSMPRSPPPDGATLCGYYIPPSAVVVTQNWSLHGNPKVWKD

PHTFDHTRWLPGSSLSEEAKMSFNPFGQGARQCLGIHLGWMQLRLATALFFRRCPGAKLAPSTTPESMVM

IDSFIAGMPKARRCAIQL

>BAC20333.1 cytochrome P450 monooxygenase [Aspergillus oryzae]

MGSGFVKAPFYKLLSPGPVDNIFNFRDAKLHSTRRKLYAKGFTLNSLRQQWEPTIRNIVALTVERIRHDA

QQGEAEILGWWTLMANETVCKLTFNGGHDTVRNGTKDPFVLMLERRMGDLAHLLQHFAPPLYYLGRLLGR

AEPRLHDVFFSQETMFEAGKHVVAIARSARDAEGDRNLFVKALAAGDLESKI

#103 63% to 62C1, 99% to 62C2

>CYP62C2 BAE55279.1 [Aspergillus oryzae] first exon differs, check

MSIKVIV

LYTAFTSPLRRVPGPLYTVLTRLPLKLASLTGNRIYFVHELHRKYGPIVRIAPDEVDISSLAE

FREIHRAGSPFLKSKWYEKFVPSKRSGVFTMRDPKEHAARRKLFARPFSKSELRRTWEPAVREKVQLAVS

QIQRELKAVGKSDLLKWWTFLATDVSGQLMFGESFNMLQLGKKNQYINVLESTMMGSGIGAELPLVAWLG

RHIPLSSFQNMFRATDYLFQYGQRAVTNSRTTSNASRNIFAGMVYESEKGDGIITDEEVVLEAGNLIVAG

SDTTAITLTYLIWAVLSQPKLQRELEEEVNSLSADFDDSALEELPLLNAVIMEALRLYGAAPGALPRETP

EGGAKFCGYFIPQGMTVTTQAYSIHRDGDIYPDPERFDVSRWLKTETSASELAKKAFSPFGAGGRICLGI

HLAWMELRLATAQFFRECAGVRLAPSATWENMRPVNYFLISPRGHQCEVECNY

#4

>CYP64A1 BAE71330.1 [Aspergillus oryzae] 99% to CYP64 Aspergillus flavus

MIYSIIICAGALLGLWILEKLLAPKDTRPPLPPGPWRKPIIGNLTDFPPKGTPEWLFWAKHQERYGPMSS

LEVMGQTIIMINDAQLGIEIMHKKSALSQMIPDAPFAHMAGWGMSLATERNRQAWKTIRANMKQEIGTRR

AISTFHSKMEIGIRRFLLRTLDNPDDLRFHIRKEANAFMMDVAYGYTIAPHGKDELYDLTQQSVRQFSHI

FSPGEWSVNFFPILRYVPSWFPGASFQIKAAEYKWTIERMTMVPYLWIKDQVARGCSRPSILLRLLQKGH

YESGSHQEQVLVWTNAEFVMGGSDTTVSAVSSLFVAMALYPEVQRKAREELDRVVGPTTLATFEHRSQLP

FIDALVKEVFRWHPASPLGAPHITQEDQIWDGYLLPKGALLLPNIWTFTHDPSVYHDPMVFKPERFLEGK

DSPPETDPMKFVFGFGRRICPGRFVTDEKLFLIACHAVSCFFISPKDPGAPEPDWLPGVISQPGTFDLNV

VPRSPAHEELIRSIETDHPWKNADATDISRFMARNQMI

>BAE59527.1 [Aspergillus oryzae]

MIYSIIICAGALLGLWILEKLLAPKDTRPPLPPGPWRKPIIGNLTDFPPKGTPEWLFWAKHQERYGPMSS

LEVMGQTIIMINDAQLGIEIMHKKSALSQMIPDAPFAHMAGWGMSLATERNRQAWKTIRANMKQEIGTRR

AISTFHSKMEIGIRRFLLRTLDNPDDLRFHIRKEANAFMMDVAYGYTIAPHGKDELYDLTQQSVRQFSHI

FSPGEWSVNFFPILRYVPSWFPGASFQIKAAEYKWTIERMTMVPYLWIKDQVARGCSRPSILLRLLQKGH

YESGSHQEQVLVWTNAEFVMGGSDTTVSAVSSLFVAMALYPEVQRKAREELDRVVGPTTLATFEHRSQLP

FIDALVKEVFRWHPASPLGAPHITQEDQIWDGYLLPKGALLLPNIWTFTHDPSVYHDPMVFKPERFLEGK

DSPPETDPMKFVFGFGRRICPGRFVTDEKLFLIACHAVSCFFISPKDPGAPEPDWLPGVISQPGTFDLNV

VPRSPAHEELIRSIETDHPWKNADATDISRFMARNQMI

#91 45% TO SEQ 26, 68% to 65T1

>CYP65T4 BAE59716.1 [Aspergillus oryzae]

MPFNILLWPVIAFFLYSLLTAIWYIYFHPLRRIPGPKSWIIFPIMRHASAIRGRFDIDMRQHHAVYGPVV

RFGRDEVSFITAEAWKDIYGHGHQQLPKVLSSASNMLDIISSNDTDHSRYRKALSHAFSAKGLQAQEPLL

NSYVDKLIERLKGIAESNLPADMVKWYNLTTFDIIGDLAFGEPFGGLDNSEYHHWVSTIFESIKAIPFLK

LKDAYPLAFKAILGLIPKGIMEARKRQLEHSRITVQKRLQTSSSYNRGDFMDSMLRNRGEKDSLNDSELE

ANSNILIIAGSETTATLLSGATYWILRNPEALAKLTDEVRSVMKSEPEITAQKASAELPYMLACFDEAFR

LYPPVPTGLQRRTLVPTRISGCDIPAGTKVSVHQSAAYWSSTNFHAPDRFIPERWLPEAKSDPSSPFYSD

NRGVVQPFSTGPRNCIGRNLAYAEMRVILARVLWNFDLELCEESTQWSDQKAYTLWEKPPLMCRLKLRES

FRG

#9 43% to 65B1

>CYP65V2 BAE61720.1 AP007163.1 [Aspergillus oryzae]

MMLNSINIPQNAISWAVACAAA (0)

198235 (0) ATFCLASSVVYNIFFHPLARYPGPLSHAASRVPYFIRVFKGTLPFD

MLDFHTRYGDVVRIAPDELAFSHPDAWKDIMGHSKGGLYLEKASWFYRP

LEYDPPHIFNEGRQAHSLLRRQLAHSFSDKGMRDQEPMIRGYVDLLLQRLREMGGREDTVDISAWYNYTT

FDIIGDLSFGESFGCLKGSNYDEWIESIFRAFPFITALQALSFTPLVKEVLLALVPPSMRDRRERLRDFT

RQKMLRRMAIAEERPDLIEGLLKKKEEL (0)

199048 VSLLTISGFDHRQAHRQ

AETLVVAGSETTATLLSGVTYLLLKNPEAYQ 199191

RLREEVRSTFSSEDDINLSSAAKLPYMLACVEEALRMYPPGA

IGPPRVTPKGGAEILGARIPGRTYVSIHHWALYRREEYFTDPHTFHPERFLGDPRFSNDRHNVLQPFHIG

PRSCLGRNLAYSEMRLILALLFFNFDMELSDDSQDWIQQKNVIMWQRGPLKVHLTYIHRNSA

#10 about 60% to seq 9, 48% to 65B2, 54% to 65V1

one stop codon and one frameshift in last exon

>CYP65V3P BAE55600.1 AP007151.1 [Aspergillus oryzae] wrong C-term, revised

MSTAALVNAKSVLIATISA (0)

PIIYMLGMAIYNVYFHPLNRFPGPVSHAISRIPYFYRAVRGTLPFD

MLKLHERYGDIVRIAPDELAFSHPDAWKDIFGHKNGEPEMAKAAWFYRPLDDPLHIVNEDTDEHRRLRRQMAHGF

SEKSMRAQEPIIRKYVDLSLEKLHQSCKNGSLVILDWYNFTTFDIIGDLAFGEPFGCLEGCNYDQWIKGI

FKGAYLGSFMQALSFVPRLKSTLLLLVPKSMQEAHQGHKALTKAKMLRRAAITEKRPDLIDGLLRNKDEL

1455183 KLGLDKLIANAEILIIGGSETTASLLSGVTYLLLQNPNAYETLKNEVRSKFNHQEEIN 1455356

LISVNKLSYILA

CLDEALRMYPPIANGLPRVCPKEGSWVLGEYIPGKTVLSIHQWALYRREKHFKDPNTYHPERFLSSPEFL

DDRRDAFQPFHTGPRNCLGRN (2)

LAYS*MRLILALVIFNFDMRLPQRAKDWIRQRNYLMWDKGPLKVYLRPRTSI*1455965

#50

>CYP65X1 BAE58707.1 [Aspergillus oryzae]

MALAAEYFSHALSQPYGSSFWVAVAASLVFICYITCDTVYNLCLSPLSSFPGPRLWAVSNIPRQLSILGG

RSHLKMLALHHRYGPVVRVGPSELSFNSPQGFRDIYGFRRGQPQFQKDPKMYGSPLTGISNSIGGHVDSD

THSRHRRLLSHSFSERSLREQEGIVVYYVDLFIQRLRERTSVNKIHRAEEDLKSWFNFTTFDIIGDLMFA

ETFDCLKDSQLHPWIALMFNNVKGIAFLGVLNEYSLFRKMQGALLPKALKQKMLENHKLCAQKADRRLQK

GASRPDFVSMQTQSCKHMGPLLFFFFMAANTDSITIAGSETTASLLSGCLFYLCKHKYIMDQLNKEIRTT

FSKDEEITSSKCFNLSYLNAVLKESLRLYPPVAASLLRLVPKGGCTIDGHFVPEDVTVSTHHYASYRDAA

NFTFPEQFIPERWLGTDNRFDSDRKDVVQPFSLGPRDCLGKNLAHMEMRLILSKLLFNFDIHLTPESENW

GQQKMFIVWDKPALMVRLTDRFA

#101 41% to 60A2, 39% to 60A5, 40% to 60B3

43% to 65S1, 40% to 65A2, 41% to 65B1, 44% to 65H1

47% to 65S3

>CYP65AB1 BAE56591.1 [Aspergillus oryzae]

MLFSLGPLTIVYGLVIFVVAKTIYNLYLHPLRSYPGPLLARATRWYYSYYVKIGLLPQKTKELHDQYGPC

VRIAPDELSYNTAEAWEDICGHRTGQRTESFEKDLTFFPPAPNGVDSIVRIDDVHRRFRRLLSHPMSDKA

LGSQQEIITGYVDQLIHELRQRSERSEVVDMVRWFNFTSFDILGDLAFGESFGCLGSGLMHPWIELIFTS

IKSVMDMQIIRRIPGLFSLILTIAGLQQKQDLQEQFMFCQKKARERYTKETTRPDFMTYILRATEEKGMT

PEEIEANAQILIMAGSETTASALSGTLFYLLKNSMAMQKLRQEIHATFQAEAEITMRSTQSMEYLHAVLQ

EAMRVYPPVPCTFPRTTPPGGAMVCGRFVPGGYIVGVNQLAAMTSEKNFKDPLKFIPERWCGDERYQEDS

RKAYQPFSYGPRNCLGKNLAYAEMRLVLTRLLWNFEFDLLEESKDWHAKQKVWMMWDKGDLKVRLKPLRH

#93 58% TO SEQ 94, 45% to 65U1

>CYP65AC1 BAE59505.1 AY510452.1 [Aspergillus oryzae]

MSQFAREIVRNAIYNTSSPDADSVSLRKATTTVLLIGVT (0)

YCILVGIYRVTLHPLAKYPGPKLAAVTRLWHSYHLCTGDIVSVLSRAHEAYGPVLRIAPD

EVLFISSRAWDDIYGARPGKPE

MDKDTPLYKGPTAPHSIVTVDGELHRFYRRLLAKGFSDAALREQEPVIQRNINLLVEKLHKEVAAGKTPE

MTAWFNYATFDLIGELAFGETYGCLENSHYHPWVEMILEVMKLRAMTHAVGYYPWIFHILMWFVPKSLRE

KFVTHRRYTHDKVQRRMDQKIHYKDLTTNLVDPQNGLERYEIDGNCSTLIIAGSETTATALSATLYFLTQ

NENAKRKVIGEIRTTFNNAGDINSISVNQLKYLSACMNEALRIFPPGPAVFPRRVPQGGDFIDGHWIPGG

TQVGIAHYCINRSRRNFVDPDKFIPERWLGDPTYQTDDRHAVQPFSYGPRNCIAHNLARLEMRLVLARLI

WEFDWELAPGSERWEEEALVFNVWSTKPLMIKFTPVAR

#94 40% to 60A2 (CYP60 is in the 65 family)

>CYP65AC2 BAE54634.1 AP007150.1 [Aspergillus oryzae]

MTLSAESSQLPWALGNIVQQVHGIPIWGT (0) HYAIYAVYNITFHPLAKYPGPKVAAMTRIVYTSHLLSGRLVEFLHQTHLKYGAVVGVAPN

EVIFTSSQAWNDIYNVRQGAPE

MSKDTPLYQSLGTPPTIAEAGHDLHRRYRRLLAKGFSEAGLREQEPVIQQKISVLIKQLHAATAKGTTPE

MTSWFNFFTFDLISELTFGESFKCQENGRFHPWILMVTQSIKFRAIIQALGYYPLLFKLFMGLIPKSYQE

AFRDHQKLTSENVQRRIDKKVDYTDLASNLIDPKHNLERYEIDGNCAVLIVAGSETTTTALSATMYYLTQ

NPEAKAKVIKEVRSSFSSAEEITAISVNQLKYLPACFNEAMRKLPPAPAVFTRRVPREGAYIAGNCIPGG

THVGMCHFATNNSSLNFKDPEKYIPERWLGDPEYEDDARAAMQVFSVGPRNCIGQNLARLELRLLLSRVI

WEFDWELDSASMDWDKDMPVYLSWGMKPLKFHFAPVVR

#104

45% to 65V1, 43% to BAE65144.1 [Aspergillus oryzae]

>CYP65AD1 BAE55230.1 [Aspergillus oryzae]

MFPCCLVPTISTSQPVEVSIAAIASWSYNVFFHPLSNYPGPRLAAATRLWYAWHCAKGSLPFAIHKLHLR

YGDVVRVAPDELSYIHPDGWNEIYGHRPGQSEIPKDPSFYSSALSSPEGIFRAPRDRHGYIRRQMSHGFS

EKSMREQEDTIRHYADLMISYLSTQANGPKENVVDFTRWYNYFTFDVMGQLVFGESFNCLQSSGFHPWVS

IIFDSIRYNVFVRCTQFWPWLSPVIRRFIPKSFQRRKIEQQALSREKANYRKTIHDGRNDLVANLLKPDS

GVTDLEYQSTVQTLIVAGSETTASLLCGVTFHLLNNPEKLEKAVKEVRSEFDSADKISFVSVNKLHYLLA

CLNEALRVYPPVADGFPRRTGSNVEVINGQPVPPNTAIRMTHWATYRSPRNFVRPNEYLPERWLGNAPGF

EKDHKNALQPFHVGPRNCIGRNLAYMEMRLLLALVLWNFDLELYPASKLWDKQRVYNLWEKPELKVKVLP

RKI

#87

40% to BAE65144.1 [Aspergillus oryzae, 39% to 65S2

>CYP65AE1 BAE60010.1 [Aspergillus oryzae]

MEALPTYLESFWHSGGGTMGISILVMLSTFLALGTIFVYRIWLHPLSGFPGPKCCSVSSIPVAWAQLRGR

NHEFVSSLHRKYGSVVRISPSELSFISGAAWNDIYSRSKGRPALERDRTFFNDMLVDPETITMANEATHS

RIRRAMAPAFSPRALLEQEPIIQANIKLLMDKLEARAGSGGAPTDLRAWFNYTTFDLIGDLAFGESFGCL

ATSTCHEWVQFVLDHFYTSTLLHVVHRFHPFNRVLAALLPKSLIEKRKAHDSMTLTKVHRRLEVQGRRND

FTQHLLDAAEAGTLSSREVEKQASVLILAGSETTSVALTFAIYLVLTNKPVLDQLNDELHSTFKEEQEIN

LLSVNQLKYLHAVIQEALRFCPPISNGFPRQTPPQGATVDGMFIPGKTVVNINHWAAYRSPRNFTLPEQF

LPERWLGDPRFDEDAKDVFQPFSVGPRNCIGKKFAYDSMKLILAKFLWRFKPTLLDKSRSWLAHQPTFVS

FHQPPLLVDLEIKGSDAFPVRE

#26 46% to 65T1 50% to 65S2

>CYP65AF1 BAE65144.1 AP007171.1 [Aspergillus oryzae]

14 P450 genes and 2 pseudogenes on this contig

MTTVPRGGIKLLSRTKQSILYLFAVIIYNVYFHPLAKFPGPKSYAATRIPYFQALLGGQIGQAIKDLHQK

YGEVVRIAPNELSFIDGEAWKAIYGTRPGHKQKPKDVRYYPPTAGGVPSIVISNDEDHSRFRRTLSHAFS

ETSLRVQEPLVNSYIDLLIQRLHEHCEAGNKPLDMVAWYNFTTFDIIGDLAFGEPFNCLQNSAYHKWVSM

IFSNIRYGTYGNLARRFPGSKFLLRLITPTRIANGRNWHIELTKEKVKDRLAKSNDRMDFYGHILKQKDT

ERAMTFDEMVTNGSTLIVAGSETTATLLSAVTFYLLKNERVLSKLQQEIRASFESEKDITVTGCNQLEYL

NAVLTEGLRIFPPTPTGLPRIVDADGDMIAGKWVPGGTIVSIPHLAAFHSASNFTEPESFIPERFLGDPR

FANDSKTVLQPFSFGPRNCIGRNLANAEMRLILARVLYNFDLELDERSENWSRQETYILWNKPGLYVRLR

PRVGI

#89 45% TO SEQ 26, 39% to 65U1, 47% to 55Z1

>CYP65AG1 BAE60004.1 [Aspergillus oryzae]

MSRRFPIQVAQSEIPPIAWGLWRMVYRLWLHPLSGYPGPRLAAVSNLPYFAWTCTGNLHLRLQELHKVYG

DVIRIRPNALTYRTPEAWTDIYGHRKPGTLPFSKDPEFFMPAQAGSSHMINANEKDHTRQKRLLNHAFSE

RSLRQQEHLIMGYIDLFIQRLRGQARMGAETVNMEEWLNFLTFDIIGDLAFGEPFGCLQNSEYHPWVATI

FKSIKTGAILRALNIYPILLGFIRRFLPKSLVQKRIAHYQMSKDRVTRRLQTETSRPDFISYILKYNDDR

GMSTPEIEMNAALLIQAGSETTATVLAACLYFLQKNAACHRRLVQDIRSAFTQETDINFLSAAQLPYMNG

VIEESLRLFPPAPGIGPRVVPKGGARICGRYVPGGVSVSVGHYSTFRSARNFTRPNEFLPQRWLDRDAES

EFASDQTMALQPFSYGPRACIGRNLAYAEMRTILAKILWHFDVQLDERSADWANSKSYIVWEKGPLWLKL

HPRNVPQETD

#110 47% TO SEQ 111, 59% to 68L1

>CYP68L2 BAE66422.1 [Aspergillus oryzae]

MLGDLFESVLTNNSVTLLITVAVAAIALHLSSPKSNLPLVNDKKPWEFRFTKARKRFLANAHNLIKAGLA

KAPAFRIVTGNGKRVVLDAKYANELRSHDDLSFGLHIADNFHAHIRGFQPFKQGSNDDEIYQNAVRMKLT

QSLGNLTQPLVDETLVALQTYWTNDTNWHAIPLKSNIQKVVAQLSSRIFLGDQICRNPNWLRIAVDYTVD

AFKAAEELRLWPKAFRAIVALFLPSCRKIRAELQEAQDIIRPVLDARRKDKQAALSAGKEPERYNDAMQW

LEECAKGRSYEPAFGQLTFSVAAIHTTSDMLTQVLYDLCGRDALIQALREEVITVVQEEGWTKPTLYKLK

LMDSVLKESQRLKPISVVSMQRVATADLRLSDGTFIPKGTSLAVSSDRMWDSEIYPNPLEFDGYRFLKLR

ELPGHETSAQVVSPSPEHMGFGFGRHACSGRFFAINEVKIALCHILLKYEFKLADGSVPRATKFGFSFTS

DPTTKLMIRRRQEETVL

#111 47% TO 68L2, 45% to 68L1, 48% to 68D1

>CYP68Q1 BAE64916.1 AP007171.1 [Aspergillus oryzae]

14 P450 genes and 2 pseudogenes on this contig

MDHFNLAGPESNTSITSLEWLGIKNSFTGSHWAHITGLSELHPTGFLCLIATLIIGIVHLTRGPKPTVLP

VVNPPGTFELTANRVKKEWLVDARQIIRRGFEKFPGKPFNMIAADVGLTTVLPPEYASEIRNNPSLSFVA

FMAHLFFSELPGFEPTREGMFDNDIGITVVHKYLTVNLARITEPLSREATAALKDIFTDNSEWHDANLKA

INLALVARLSSRIFLGEELCRNEEWLKITVNYTVDVMKAAERLRRVPGPLRRIVHWFLPEAQKCRDEVKR

AGKVIRPVLEKRRREKATMESEGKEALQYNDAIEWFEQMAKSQGTSYDPEVVQLFLSTVAIHTTSDLLTV

VMADLARNPEIIEPLREEISSVLRDGGWKKTSLTDMKLLDSVLKESLRLKPIAVVSMRRVAMDHLKLSDG

TFLPKGTKMAVSSHRMWDPDVYENPEQWDGFRYVNLRETPGQDKHAQFVSTSERHLGFGHGKHACPGRFF

ASSELKVALCHILMKYDFELAPGTVVQHRYSGASYYADPAIRVMLRRRNVALPSWFER

#95 45% TO SEQ 96 86% to 504A1

>CYP504A8 BAE58419.1 [Aspergillus oryzae]

MSLQTIAIAAIAVAYFIIRYLNRTDVPKIKGIPEIPGVPLFGNLLQLGDQHATVAGKWAKKFGPVFQVRM

GNKRVVFANSFDSVRQLWIKDQAALISRPTFHSFHSVVSSSQGFTIGTSPWDESCKRRRKAAATALNRPA

VQSYMPIIDLEATASIKELLKDSQNGTVDVNPTAYFQRFALNTSLTLNYGFRIEGNVDDELLKEIVDVER

GVSNFRSTSNNWQDYIPLLRIIPKMNREAEEFRVRRDKYLTYLLDILKDRIAKGTDTPCITGNILKDPEA

KLNEAEVKSICLTMVSAGLDTVPGNLIMGIAYLASEDGQRIQQKAYDEIMKVYPDGDAWEKCLVEEKVPY

VSALVKETLRFWTVIPICLPRVNIKEIEFNGAKIPAGTTFFMNAWAADYDEDHFKMPEKFLPERYLEVSE

GSGTPHYGYGAGSRMCAGSHLANRELYTAYIRLITAFTMHPAQNPADRPILDAIECNEIPTALTTEPKPF

KVGFKPRDAPKLQQWIMESDERTKDL

#96 82% to 504B1

>CYP504B6 BAE56546.1 [Aspergillus oryzae]

MAITATLFSIQKSLIHTPLQSIAIAALFTSCLYFIVNEFIRAFSRVPGFKGPRGLPLIGNIAQIRKNAAE

QYRIWSKTYGPVYQIQLGNIPVIVVNSAVSAKAIFGQNAQALSSRPEFYTFHKIVSNTAGTTIGTSPYSD

SLKRRRKGAASALNRPSVESYVSHLDAESKAFVAELLKYGEGGNVPVDPMAMIQRLSLSLALTLNWGVRV

VSQEEDLFNEITHVEEEISKFRSTTGNLQDYIPLLRLNPFNTNSKKAKEMRDRRDKYLNGLNRDLDDRME

KGIHKPCIQANVILDQEAKLNSEELTSISLTMLSGGLDTVTTLVAWSIGLLAKRPDIQDKAAEAILNMYG

PNQPMCDAADDQRCAYVAALVRECLRYFTVLRLALPRTSIRDITYGGNIIPKGTVFFLNSWACNMDPDTW

SDPEEFRPERWLEQPDAPLFTYGLGYRMCAGSLLANRELYLIFMRTINSFRIEPYDEIDWHPVHGNADPT

SLVAIPKKYKVRFIPRDNKVLEDVLGSAFSLKRVI

#41 56% to 505A8

>CYP505A3 BAC55896.1 fatty acid hydroxylase [Aspergillus oryzae]

MSTPKAEPVPIPGPRGVPLMGNILDIESEIPLRSLEMMADTYGPIYRLTTFGFSRCMISSHELAAEVFDE

ERFTKKIMAGLSELRHGIHDGLFTAHMGEENWEIAHRVLMPAFGPLNIQNMFDEMHDIATQLVMKWARQG

PKQKIMVTDDFTRLTLDTIALCAMGTRFNSFYSEEMHPFVDAMVGMLKTAGDRSRRPGLVNNLPTTENNK

YWEDIDYLRNLCKELVDTRKKNPTDKKDLLNALINGRDPKTGKGMSYDSIIDNMITFLIAGHETTSGLLS

FAFYNMLKNPQAYQKAQEEVDRVIGRRRITVEDLQKLPYITAVMRETLRLTPTAPAIAVGPHPTKNHEDP

VTLGNGKYVLGKDEPCALLLGKIQRDPKVYGPDAEEFKPERMLDEHFNKLPKHAWKPFGNGMRACIGRPF

AWQEALLVIAMLLQNFNFQMDDPSYNIQLKQTLTIKPNHFYMRAALREGLDAVHLGSALSASSSEHADHA

AGHGKAGAAKKGADLKPMHVYYGSNTGTCEAFARRLADDATSYGYSAEVESLDSAKDSIPKNGPVVFITA

SYEGQPPDNAAHFFEWLSALKGDKPLDGVNYAVFGCGHHDWQTTFYRIPKEVNRLVGENGANRLCEIGLA

DTANADIVTDFDTWGETSFWPAVAAKFGSNTQGSQKSSTFRVEVSSGHRATTLGLQLQEGLVVENTLLTQ

AGVPAKRTIRFKLPTDTQYKCGDYLAILPVNPSTVVRKVMSRFDLPWDAVLRIEKASPSSSKHISIPMDT

QVSAYDLFATYVELSQPASKRDLAVLADAAAVDPETQAELQAIASDPARFAEISQKRISVLDLLLQYPSI

NLAIGDFVAMLPPMRVRQYSISSSPLVDPTECSITFSVLKAPSLAALTKEDEYLGVASTYLSELRSGERV

QLSVRLSHTGFKPPTELSTPMIMACAGSGLAPFRGFVMDRAEKIRGRRSSGSMPEQPAKAILYAGCRTQG

KDDIHADELAEWEKIGAVEVRRAYSRPSDGSKGTHVQDLMMEDKKELIDLFESGARIYVCGTPGVGNAVR

DSIKSMFLERREEIRRIAKEKGEPVSDDDEETAFEKFLDDMKTKERYTTDVFA

>CYP505A3 BAE56963.1 [Aspergillus oryzae]

MSTPKAEPVPIPGPRGVPLMGNILDIESEIPLRSLEMMADTYGPIYRLTTFGFSRCMISSHELAAEVFDE

ERFTKKIMAGLSELRHGIHDGLFTAHMGEENWEIAHRVLMPAFGPLNIQNMFDEMHDIATQLVMKWARQG

PKQKIMVTDDFTRLTLDTIALCAMGTRFNSFYSEEMHPFVDAMVGMLKTAGDRSRRPGLVNNLPTTENNK

YWEDIDYLRNLCKELVDTRKKNPTDKKDLLNALINGRDPKTGKGMSYDSIIDNMITFLIAGHETTSGSLS

FAFYNMLKNPQAYQKAQEEVDRVIGRRRITVEDLQKLPYITAVMRETLRLTPTAPAIAVGPHPTKNHEDP

VTLGNGKYVLGKDEPCALLLGKIQRDPKVYGPDAEEFKPERMLDEHFNKLPKHAWKPFGNGMRACIGRPF

AWQEALLVIAMLLQNFNFQMDDPSYNIQLKQTLTIKPNHFYMRAALREGLDAVHLGSALSASSSEHADHA

AGHGKAGAAKKGADLKPMHVYYGSNTGTCEAFARRLADDATSYGYSAEVESLDSAKDSIPKNGPVVFITA

SYEGQPPDNAAHFFEWLSALKGDKPLDGVNYAVFGCGHHDWQTTFYRIPKEVNRLVGENGANRLCEIGLA

DTANADIVTDFDTWGETSFWPAVAAKFGSNTQGSQKSSTFRVEVSSGHRATTLGLQLQEGLVVENTLLTQ

AGVPAKRTIRFKLPTDTQYKCGDYLAILPVNPSTVVRKVMSRFDLPWDAVLRIEKASPSSSKHISIPMDT

QVSAYDLFATYVELSQPASKRDLAVLADAAAVDPETQAELQAIASDPARFAEISQKRISVLDLLLQYPSI

NLAIGDFVAMLPPMRVRQYSISSSPLVDPTECSITFSVLKAPSLAALTKEDEYLGVASTYLSELRSGERV

QLSVRPSHTGFKPPTELSTPMIMACAGSGLAPFRGFVMDRAEKIRGRRSSGSMPEQPAKAILYAGCRTQG

KDDIHADELAEWEKIGAVEVRRAYSRPSDGSKGTHVQDLMMEDKKELIDLFESGARIYVCGTPGVGNAVR

DSIKSMFLERREEIRRIAKEKGEPVSDDDEETAFEKFLDDMKTKERYTTDIFA

#157 61% TO 505A3

>CYP505A14 BAE56245.1 [Aspergillus oryzae]

MEETKLIPIPEPRGLPLLGNILDVDSEAPEKSFQRLAETYGPIFRLNLAGASRVFISTYELVDEICDEER

FTKVVTAGLREIRNGVQDGLFTADYPGEDNWAIAHRVLVPAFGPLMIRAMYEDMYDIASQLALKWARQGS

SATIMANDDFTRLTLDTIALCSMGTRFNSFYSEDLHPFIKAVATLLQGSSDRTFRSTLLNNLPTRENKKY

WSDISLLRTLSQELVDARRNNPIDKKDLLNALILGQDLQTGQHLSDDSIINNMITFLVAGKGVCVGHETT

SATLTFLFYYLLKNPHAYQRAQEEVDTVVGQRKIIVEDLSKLPYIAASLRETLRLQAPVPLIAFHPHPTK

NHEDPVTLGKGKYALNNDEPVVLIMGKVHRDPKVFGDDAEEFKPERMLDKNFEDLPKNAWKPFGNGMRGC

IGRPFAWQEMLLVVAMLLQNLNFEMENPSYDLRIKQSLSIKPDGFQMKATLRRGLDAAKLASVLNSGGDL

LSHAPQILNGEYKPNTDLRFHLRPMHIFFGSNTGTCEALARRLAKDSMGYGFATRVESLNSAMENIPRDN

PVIFITATYEGQPPDNAAHFFEWLNGLKKAELDGVNYCVFGCGHHDWSATFLRIPKATNDLIEKHGGTRL

CDMGMADAANSDMFSDFDTWSELILWPAINLKFGRASSEGDVQSKSALHVDVSSSMRAFTLGLQLQEGYV

LENKLLTTPDVPAKRMLRFKLPPDTTYQCGDYLIVLPVNPAHVVCRAIRRFNISWDSMLTVRKPSHASDG

ITNMPLETPISAFELFSTYVELSQPASKRDLITLADAATTDTDAQAELQSLASSPNRFTEEVINNRLSPL

DILIRHPSINLPLSTFLEMLPPLRARQYSISSSPLASRSDCTITFSVLNSPHLSTENKRFVGVASTYLSE

LQAGDRVQISIRASNNKGFKPPLKEETPMIMACAGSGLAPFRGFIMDRAERIRGRGTELLSDDDHPEIGK

PARAILYIGCRTKGKDDIHASELDEWTRQGAVDVRWAYSRPTDRSQGRHVQDLLFEDRNELLELIDQGAR

IYVCGGMSVGQGIRQVFKDMFIERCREVLENGSDGDEDVAAEEYLDSLKTEERYATDVFT

#40 45% to seq 41, 50% to 505A8, 54% to 505C2, 47% to 505B1

>CYP505C3 BAE63564.1 [Aspergillus oryzae]

MRQNDNEKQICPIPGPQGLPFLGNILDIDLDNGTMSTLKIAKTYYPIFKFTFAGETSIVINSVALLSELC

DETRFHKHVSFGLELLRSGTHDGLFTAYDHEKNWELAHRLLVPAFGPLRIREMFPQMHDIAQQLCLKWQR

YGPRRPLNLVDDFTRTTLDTIALCAMGYRFNSFYSEGDFHPFIKSMVRFLKEAETQATLPSFISNLRVRA

KRRTQLDIDLMRTVCREIVTERRQTNLDHKNDLLDTMLTSRDSLSGDALSDESIIDNILTFLVAGHETTS

GLLSFAVYYLLTTPDAMAKAAHEVDDVVGDQELTIEHLSMLKYLNAILRETLRLMPTAPGFSVTPYKPEI

IGGKYEVKPGDSLDVFLAAVHRDPAVYGSDADEFRPERMSDEHFQKLPANSWKPFGNGKRSCIGRAFAWQ

EALMILALILQSFSLNLVDRGYTLKLKESLTIKPDNLWAYATPRPGRNVLHTRLALQTNSTHPEGLMSLK

HETVESQPATILYGSNSGTCEALAHRLAIEMSSKGRFVCKVQPMDAIEHRRLPRGQPVIIITGSYDGRPP

ENARHFVKWLQSLKGNDLEGIQYAVFGCGLPGHHDWSTTFYKIPTLIDTIMAEHGGARLAPRGSADTAED

DPFAELESWSERSVWPGLEAAFDLVRHNSSDGTGKSTRITIRSPYTLRAAHETAVVHQVRVLTSAETTKK

VHVELALPDTINYRPGDHLAILPLNSRQSVQRVLSLFQIGSDTILYMTSSSATSLPTDTPISAHDLLSGY

VELNQVATPTSLRSLAAKATDEKTAEYLEALATDRYTTEVRGNHLSLLDILESYSVPSIEIQHYIQMLPL

LRPRQYTISSSPRLNRGQASLTVSVMERADVGGPRNCAGVASNYLASCTPGSILRVSLRQANPDFRLPDE

SCSHPIIMVAAGSGIAPFRAFVQERSVRQKEGIILPPAFLFFGCRRADLDDLYREELDAFEEQGVVTLFR

AFSRAQSESHGCKYVQDLLWMERVRVKTLWGQDAKVFVCGSVRMNEGVKAIISKIVSPTPTEELARRYIA

ETFI

#20 58% to 531C2

>CYP531C3 BAE65935.1 [Aspergillus oryzae]

MDSFLFLALDYSLRGFAVSLFGTLWIALVFWYLATYLISPLRKIPGPFLAGWTNLWRMYHVTQGQSQVVL

HELHQKYGPVVRIAPNVVDLDLPEMIKTIYNTKGDYRKTEFYHGSSAKSNGRIIYNLFSECDPDIHAQQK

RPIAKYYSLTGVLPLEPHIDEVINYLCQRLEEEFINGLNAGITCKLDQWLLFYTWDVVGQATFSEPIGYL

KNGFDFDGTIAISDTAMDYFSLVGQLPVLDHLLDKNPIYRIGPPAFGNITNISITHLLDRLQGKDTSYHD

ANKPDFLDRFIDAKDKYPDIVDDSQIISYLMINMIAGADTTAITLNAAIYFALKDRRVWARLQKEIRACQ

SSLDAAPSAVPYNIASALPYLNAVVREAMRMHPGVAMTLERYVPPGGLTLPNGQYIPQGSIVGMNPYVIA

RNRSVWGEDSDVFRPERWLRDDSQESEEEFQARLRLMNNSDLAFGAGSRICIGRNLGLLEVYKVMATLIS

RYDIELAHPHRDWKTHNSFFVRQEGINVKLSRRS

#39 45% to 531D2, 40% to 531D1, 39% to 531C1, 40% to 531A1

44% to 531D4, 42% to 531D3, 38% to 531C2, 37% to 531B1

>CYP531E1 BAE63827.1 [Aspergillus oryzae]

MLSITELIMDLGLAIVILGVSHLLWNYLSSPLKAFPGPLWASFTNLWRLQDVFKGRCDITHNQLHRKYGT

AVRMGPKVLSLSDPSVIPHVFNSKNPWMKSDMYNVNDVIVSGVRLKNLFSHQDEKWHSTYIRPVKGLYSM

TKVQDMEPGVDVTINLFMDKLRERFVEKGQLCDMADYLNFFAWDVMSQITFSQNLGILEAGSDYQGFLGR

SNKSLDYFASISQMPILDLLLDKNPIVRLGPPTFVWANIFSLEQLQKRLSGGTPPSGHTDFLDKFLETKK

KYPDLVNDNTVVTYLLSNTLAGSDTTGSAMCSAVYHILKHPRVHRKLREELYAAKVPLPAKWKDLQGLTY

LDAVMRESMRVNPGVGLMIERIVPEGGFTLPDGRFIPAGTIVGMNPWVINKNEAIFGANTEEFIPERWLP

SPGESDQAYQARFTKMKSTDFTFGAGPRMCMGRYLSQLESYKLIATLFSTFEMELPSLDHTWHVTNSWFV

RHKSIPVKMRERTDLAVSV

#142 52% TO SEQ 39, 40% to 531D2

60% to 531E1 after making adjustments for gaps

>CYP531E2 BAE59053.1 [Aspergillus oryzae]

MDIAFKLVLLGATLILSHLIWNYFRSPLKSFPGPFSANLSNLWRLQDVFKGLCDITHNELHRRYRSAVCI

GPNVLSLSDPALINQVYSTRDPWVKVSSERTQDPQHSDVYNVNDSVVSGVRIKNLFSHQDEQWHANQELE

PGVDITINLFLESEYINYFTWDTMSQLSYSQSIGMLEAKNDRFGILEVSNRSLDYFASPKTLISSFLQVC

QIPMLDLLLDKNPICRLGPPSFGWSVKFSAEQYQERFTEGKQSHNGIKDFLDRYIETKSKMPNIVDDNVA

QMYLVLNIIAGSDTTARAISAAVYYEELRGANLSTPPQWKEIRSLPYLDAVMRETMLVHPGVGLLLERIV

PKGGFTLPDGRFVPEGTIVGMNPWVINRNRTVFGPEPDSFKPERWLPAEGEHDEAYQTRFSKMKGTDLTF

GAGPRACLGRYISQLESYKFVATLFTMFDVSFGCLQDLEHPDHEWEVTNSWFVRQKNIPVVIQKKKVLNC

TPSVQWASTALMYMPQRESFSRALPPLHCGQKFQIGTSKLQHD

#12 74% to 532A4

>CYP532A6 BAE66552.1 [Aspergillus oryzae]

MTLLAEVFSRASPEYALYILPFISLIYIGHLYFHNGLNRYPGPFLAKFTDLWRFLDVWGRQPHETHIALH

RKHGDVVRIGPNTLSFSSPAATKVIYGLNKGFTKSEFYPVQMTVSKGEPLPSLFSTLDDKFHAELRRSVN

HAFSMSSLVQYEPMVDETTLLFLDQTDRLFATGGKVCDFARWLQFFAFDVIGSITYSKRHGFIEKNEDID

GIVKSLARIFNYAGPVGQMPWLDKVFWKNPIFDAMQKWGLLDNSHPVAIFARQRMMERMSSKAAIDPSSR

SDLLTKFMKAGELRPNFMTEKRVLTMAVSMAFAGSETTAISLAAVFYYLLKTPDYMRRVREELDEAIQNG

TIENRPSGLVSWTESQKLPFLDACIKEAFRIFPAAGLPLERVTPPSGADIAGQFIPGGTIVGCSPWVIHR

REDIFGSDVDTYNPDRWLNASEDKLKIMNGMMLQFGAGSRTCIGKNISLMEIYKLIPSFLRRFDVRLAYP

EQEWRLWNAWFVRQYNFNTVFTPRKIEVQ

#137 46% to 535D1, 49% to 535C1 Mg, 47% to 535A1

52% to 535D2, 44% to 535B1 Mg

>CYP535E1 BAE61757.1 [Aspergillus oryzae]

MGLVELVLETRWLIVGSLFILYFLKKFQAYYRLRKFKGPLSCGFSHFLHTKAVLSLRCERWYKEMTDQYG

SIVRIGPNALITSSPELWAHINAVRSPYKRSDWYYHAARFKPGEDHVFSETNNERHDRRRKQMLMGYSGK

ENLSLESDIDLRVLDFLDLIRNRYLSTEDCLKPMDLAKKVQYLTLDVISTVGLGNSFGMLKADKDVNDYI

KSGEEGLWVSNFLMGTGLHWIMQIEWVGRLLGPSTDDLKGFGKMMATTGQMVAKRLQSPTNARSDMLASF

IRHGLIGNELWMEAFEQVLAGSDTTASGIRGILLCLLSNPRVYKKLQAEIDGAVQDGRAPSTGIISDAQL

RRLEYLQAVIREGLRIFVPVVNIFARDVPPEGDEVIVDGESVQIPGGTWIGYSALGMHLNKATYGEDAEV

FRPERWLIDDKDHLANMTRVNDLIFGYGRWKCLGQTVALIEIGKTIFEVHDELPLLTIVGLDILTVLEGA

A

#107 67% to 537B1

>CYP537B2 BAE66494.1 [Aspergillus oryzae]

MWSLWLSSILLVGLYLFKRLSSPLAKVPGPWYTNLTSFCLKYHEFTATRRLFVHRLHKKYGPVVRLAPNE

VSFASLDAIREIYASGGSGYDKTEYYDLFRQYGIKYAMSNIVRDKHVSAIKERAQAFVSRCAAIEGSVNV

YSLLHCYALDGVTNFMFSPGGLKSLDNTKDYEMMEELTYHQSLQKNLLYYYLPRLAPYFPSCLHPRPAPR

ANAYVLQMAGQQHPEAHSLVARLTRKGSPLSHMQVAAECKDHMAAGIDTTGDALCFLMWELSQPQNLQFQ

DRLHKELLSTSDDTPLDKMSYLDAVIKEALRCAPPIPMSIPRYVPSGGRTIDGYFIPENTIVSCQPYTVH

RFNEEVFPEPDRFNPERWLEEKGFNDRNRLFFAFGTGGRGCTGKNLAMVEMKILLRELYSRFRSSVAPDM

TASMDLDDQIISARPKDQICKLNFAVRGDDVDTA

#99 56% TO SEQ 75, 84% to 540A2

>CYP540A3 BAE57130.1 [Aspergillus oryzae]

MKCQFYLVGDDIATAQSILVDSRWKFEDLQRAVGGIFHVALPTGISFHTSENETLSSVADIISASSSPIG

LRIDGNAVQTPQGPKGLPLVGSFYEIFPDHLGNHYRLFRKYGPVIKTTNMGKTTYLTDDPQVASVCLAES

AYMTKKINENHPLWGVKDNTAIFIGDTETENWRLAHKYLPPAMGPKAVRHYTGLMQNCARKSLPVFDELD

GRDESWNVYQYMVKLASQTIGSFSLGKDFGHFDSVDSPLHPIVTNIANLLSLNKKITARGEWYRYLPFGD

PARLRHVQHTIYTLLQEAIDEVAGSGIADAPMNEAALSASCVVDYLLHAVDDKGEHFPQGLILANMLIVT

GAGFTTTSALLSWLLYCLVTYVGTQDRLYAELVEHGIVGPSGERNQTTWTPDLAHSLPYLDKFVKETQRL

HNASFQPGRTTKTDVVLPGGYRLPPDSVIVPALYAIHTNPKTWRDPFRFDPDRWDTEEVKGRHRCAYIPF

ATGPRGCIGFNFALLEVKILLAELVSRYEFVRDGLEAIDYDPEFQLIRPLNFYVRAKRRV

#75 80% TO SEQ 76, 69% to 540B1

>CYP540B9 BAE62604.1 [Aspergillus oryzae]

MAAADSISITIDGKSVREISGPKGLPIVGNFFEIYPDHLGNHQRLFEQYGPIIKTTNMGRTVYQTNDPQL

SSIIFSETDFFTKKINEAHPLHAIKNQQAGVFLGDTDTPEWRAAHKFLPPALGPKAVRHYAPTMQQTVED

AFKVFDHFDENGEAWNVYQYMLKLGSQAVGKLVLGIDFNHFSSPDAPPHELIQIIAESLSLNKKVTARGD

WYAKLPFGDPQRLRNMRYRIFDIVDQSIQNASRNGVEDLPLQDAALKASNMIDYAVRATDNKGEKLPKTS

LIQSLVVATGAGFTTTSSLLSWLIYSLVTYPGVQDRLLQELIDNDITEDTQITADLTDRLTFMDKLIKET

QRRHNPSYQPARTAKVDMILPGGYKLPEESVVVGALHHLHNNPEVWSNPTRFDPDRWDTEEVKNRHKAAY

IPFATGPRMCIGFNFALQEVKVFLPKLVYRYKFTKENDGPIEYDPMFQLIRPNNLYVRAERRVKWPPKSE

SATTRASL

#76 80% TO SEQ 75

>CYP540B10 BAE59862.1 [Aspergillus oryzae]

MTQLRIVEVGSRNSEGIDKDFSDSKVREPPGPKGLPIVGNFFEVYPDHLGNHQRLFEQYGPIIKTTSLGR

TTYQTNDPVLSSIVFAESDFFTKKINEAHPLYALKQPSAGVFLGDTETPEWKVAHKFLPPALGPKAVRHY

APTMQETVEDAFKVFDEFDKQGEAWNVYQYMLKLGSQAVGKLTLGLDFQHFTSPDANLHEMVNLIAELLS

LNKKVTSKGDWYAKLPFGDPQKLKQIKVRIIEMVEESIRSAERGGVVDLPLQDAALQASNMVDYAVRATD

NKGEKLPKTSLVWALTVATGAGFTTTSSLLSWLIYGLVTYPDMQERLLQELIDHDIDENTQLTADITDRL

TFLDKYIKETQRRHNPSFQPGRTAKVDLILPGGYKIPQDSVIIPALHHIHNNPELWDNPQKFNPDRWDTE

EVKNRHKAAYIPFAMGPRMCIGFNFALQEIKVFLPKLIYRYKFTREGDGHIEYDPMFQLIRPNNLYVRAE

RRVKWPPKTEN

#51

>CYP541B3 BAE61440.1 [Aspergillus oryzae]

MPSPIPKPKGVPILGNVFDLDGNNTWGSFNKLAKDYRPIFKINILGKDIVFITGAHLLEEVCDETRFRKC

VAGPILAIRDAVNDSLFTAKHTTEEIISWGIAHRIMAPLVSWEAVEQVFSGIQETTDDLIKKWTTGSRQR

INVTNDLDRCNHAANMLCFFDQRVHIMEGPEPAVIKAMGCATNEAMHRPFRLRIVNWLFHSRKWNAWIKT

MRDYGKDIVETRRANPTDKKDMLYALLEGKDPETGKGLTDSQVLDEIINIFIGSATAPNLIAFAMYYLAN

NPDAAAKAREELDAVVGGPSVRIEHEHLARLPYTEGVLRESFRLSGVAPGFNIEPIPENEGPVLLAGGEY

EVPKGQALIAILAAVNRDPAVFEDPEAFKPERMVGENFDRLPKGVKKGFGNGKRECIGKRYAWQWSFMVL

ASILKDVEFELADPNYKVGNDGVNCNGAFSVRPQEMFVVTGPRARAA

#11 73% to 547C1

>CYP547C3 BAE66568.1 [Aspergillus oryzae]

MDLMLALITLSLVEGFLLQRTVFVDLTFRTVTLSSVGVNLVLLTIWNTLIYPYFVTPLRHLPTISGNLNN

ARIVFDDPRGRLPLEWMKTVPNEGLIHFRDIVNRSYLLATNHQALLDIMSTNTYDFEKPWRARDFLARII

GFGLILSEGSAHKKQRRALTPSFNVKNIRALYSLMWEKTGLLMDELEKQIKQNPMDGTNAQSGEGKIEMS

VWASRLTLDIIGPAAMGRDFRSLHNPENKVADSFLAILEPTKEKMAFLAVNFALPQWFARRIPWRLNKVI

NDETGFLRDLCRDIVHEKRASITATKATAKELEADILGTMMLGGDFTDDELIDQMLTFLAAGHETTASAF

TWACYLLTLYPDVQERLRTEIRERIPSGNHPITWSDLETLPLLNGVCQEVLRLYPTVPMTIREAVRDSVV

AGKHIPKGTRILLCPYAINRSPEFWGNDGEEFRPERWIDTDKNGQLVTNNTGGASTNFAQITFLHGQRSC

IGKDFARAELRCAVAGVVGRFRFEMQNPKQKIHIAGAVTTKPVEGMHLRMSRVDDW

#72 45% to 548A2, 81% to 548D2

>CYP548D3 BAE62732.1 [Aspergillus oryzae]

MLVQEVWKTLSIEGVVVGVSALLGLLSFAYVIYNRFLHPLRKFNGPFLASITPWVQLYHGLKGDRHLWLH

NLHQQYGSHVRVAPNFVSINSAQGLHDIYGHGKKLKKANFYNGFTAIKGVYNTHNVIDKTVHGRKRRVLS

QAFSDQALKSMEDVMLLHVRQLCTALAEQQTEGNKAEAQKTTVQNMGDWFSYLTYDVMGELCFGKSFDML

VSSGRRKMIQLVDRAANRHYVCGLWMPLDTWHLDQIVIHRLTNDRWNFIMNSRVEANKRAQERTQAGHDS

KKDFFYYLLNAKDPETGKGLTTPELWGEANVLMIAGSDTTSTTLAATIFYLVRNPRAMKLLKKEVREAFT

SVEEIVTGGKLNELVYLKACIDEALRLAPAVPGAIPREVMEGGAVVDGAFLPAGTDCGTPTYSIHRQPQY

YREPEAYLPERWIEGATCVTADDKWESTKDAVETARRAFCPFSIGPRGCIGKGMAFMEMRLTIARLMFLF

DMELADRQGEDEKGHLALVDHFTSAKQGPNVIVRRRDITV

#52

>CYP548F1 BAE58762.1 [Aspergillus oryzae]

MQVGGLRSRVSVLVAIQLVSLIVYRIYFHPLSKIPGPLLPKITDWYPAWCVWRGSSHTALWEGHRRYGSI

GRCGPNSVSVCSQTGLMEIYSTKANVCKDESYVVMSVGSHAPNTFSFIDKKTHAFRRKILFQAFTDNALN

GVQDQILSHISEFCAMLNPPPSNGAGQSSVWGPSVDIAPLCDYLAFDVISDLSYGRSFGMLKSDRYRYVP

KLTRRLARRNATCMTQSKLWRYKLDRLFFAGFLKALRDFGLWIRHQGKERIRLGNNGPRKDCFHYLLSGS

DPKTGQGLTERELRVELLLLIVAGSDTVATSLSAVLFNLAHNQQALQKATAEIRSCFEREEDIRLGTRLK

SCSYLHACISESLRISPAVSNMPPRRVLPGGITVDGYYIPEGTIIGTPIYALHHNEEYFPRPFKYEPERW

LENEANGENPSTDDGLKRARAAFCPFSIGPRSCVAKNLAWAELTLTLARVLFSYDVRLPPDHCEVEPDCC

SSVPRDQSPEYKLRTWIVSAREGPSLQFRPRNVKVP

#37 45% to 548C1, 50% to 548B1

>CYP548H1 AP007171.1 BAE64607.1 [Aspergillus oryzae]

14 P450 genes and 2 pseudogenes on this contig

MQAVALLRHVPLFAYVTAVPVALLAYVLAICIYRIWFHPLAKYPGPLLAKVTNLYGGYYAWKGDLHIDMM

RCHEKYGNYVRYAPNRVLFNTNTGLKEIYAYSKSFQKSAAYGAMVHRAPNTLTLIDKKQHGRKRRIIGQG

FGDAALRGFEGTIMSLVRKFCDELAKDISGRKAGEWSTPQNMGKWSNYLTFDIMSGIIFGESFDLIGSPK

NREIVKCIEDSNVRTGVLSQAGELSTRRLDRWLFPQAIQGRNAFIRFVNILLKKRMSAKPLKRHDAFSFL

LDAVDPETQQGFTPAEIGAESTTMIVAGSDTSSTAIASTFFYLCRNREWYEKAKEEVRAAFPGPDDVALG

PALNNCVILRACIDESLRMSPPASSSLWREVLDDGVIIDGQVVPRGYDVGTCIYAIQHNPDYYPEPFEYR

PDRWLDPVPEKVQLARSAFSPFSIGPRSCLGKGLAWTELMLTMAYMLSKYEFRSAPGEDEKVGGGHVDMG

AGRQREGEYQLRDHVTAAKDGPIVQVSFR

#73

53% to 551A1 43% to 551B1 Fg CYP53 clan

>CYP551A2 BAE62699.1 [Aspergillus oryzae]

Note 551 is not in the tree on part C where it belongs

MEDTFNLTVLGGVIVLYSLGLVIYRLYFSPLAKFPGSKLAAATGWYEFYFDYWIGGKYVFEIERMHQVYG

PIIRINPHELSIRDPDFYNEIYVTENKRRSNHYDLFARGIGMDDARVESHIVTVDHNLHRKRRKPLEPFF

SRAGIARLEPALVEMSLKLESRLRQYEGKHTVVTLDHAFSAFSGDIIRRVCFNKDDFGDLFMDHPDFSPD

WYNLIHNILRHFPVFTGFPLIARIVTYIPESILLKAFPQGQSLNRLKDVALQRITHVMNSKATELKDTHR

EVSLFHHIVESDMPESERSPERLVQEAQVLLSAGTVTTARTIAFASFYILARSEIKAKLQAELRDAMDGW

PEKVPTFMDLERLQYLQAIIKESLRLSYGIMHRLPRVSPDLPIQYKDFTIPVGTPVGMSSYFMHTDPTVY

PDPMTFRPERWLGAIDPAMHRNYVPFTRGSRNCLGMNLAMAEISLILAVLYRPNGLKLELYETDETDVMI

AHDFMAPMPKVTSKGVRVLIR

>CYP567D1 AN3281.2 AACD01000055 revised

N-term may be wrong 41% to 567B1 53 clan

MSASWVDEPSSLLGIFEVVFVL (0)

TLVYVSTRILYNIYFHPLAAFPGPLLARASM (0)

AWRIYHSMGGRFHRAIDEQHRRYGPVFRVSPNELSFASVASWKDIYGHRAT

GKPPLIKSKFYEIYGAGFGSLCIGSERDPEKHTRMKKSLSPAFSLRSLGD

QEVIVSQCVDRFVSRMGEPELNAGGLNMTKWYEMVAFDILGEL (1)

AFGESFHSIED (1)

DHLYFITVADNFRHFPVVALIAKLLSPFLDSIRKKHTGYTRNKVAQ (2)

RIRSSSPRKDFMANLIGKVESNEMSREELTAHASTLV ()

IAGGETVATFLAATTFYLLRDD

AVYQKLKAEIRGQFNTYEEITCATAQNLPYLQAVISEGLRIYPPGSQGFP

RISPGASIDGIWIPAGA (0)

EVYTSAWTVTHDEANFHDPHTFKPERWIDPDCADIKEASQPFSLGPRACLGRN (2)

VAYMEINLILARLIWTYDMELVNKSMDWESESSLHVMWSKPELKVDLEVSTLGNCAAGGD*

#36 59% to 567D1, some errors

>CYP567D2 BAE64652.1 AP007171.1 [Aspergillus oryzae]

14 P450 genes and 2 pseudogenes on this contig

MDRVVWLGLAVA (0)

286366 LTALYWVHLVIYNVFFHPLASFPGPFWARASFLYPNILASDMANVAYFHWPCSSRHRIS

TQSSWWALTDPPIYSNIINPGL

RLGDIVRISPNELSFASVESWKAIYQPKSAPLVKSEFYEIYGSGFNSLCIGSE

RNPETHSRMRKSLAAAFSTKALLEQEDIIQGCVNDFIEGIRSQTTVNFTKWFEMLAFDILGEMAFGESFHCIEN

EHLYFITILDNLRRYPLIAAIGKTILPHLTVSVRNKHTNYSRRKVA

HRLQSSSPRADFMSRLIAKVEDEEMEMEELTAHASTLV

IAGGETVATFLAAVTYHLLSTPN

AYQKLRDEIRARYNHLSEITSTTALQLPYLQAVISEGLRIYPPGSQGFPRNTPPQGIVVKGTYVPGNVEV

YTSAWTVTHDARYFHDPYTFKPERWLDPNCTDNKDASQPFSLGPRGCLGRNFAIVEMSLILCKLHFQFDA

ELVNPFQEWESASQLHVMWWKPDLPVRFIPCTRDH

#53

>CYP573A5 BAE58632.1 [Aspergillus oryzae]

MVLAGEDLVAMFTVTHVILALGAYVALRFAYQIVYYRFFHPLSVFPGPFWGSVTRLWIAWHNLRETEVPT

VYALTKKYGPVVRITPTLLLVSDPTKLPDIYHRNADKTGHYITGSFGETESLFNIRSHKTHAAFRKHIAG

PYSFSNVTRMEPLVDARIDDWLNKLDETFASTGAKFDFAWWAVYVAYDVISEIGFGAPFGFIEQGKDVGG

LIQGFHDGLPAFGLLARLHPFTSWMKTTFMKKYLVAKPEDNTGIGVLMRFRDRLIEQRLREIAEGKDVGR

VDLLQTFIDARTEDGKPLDMDYLKAEVLLVLLAGADTTGTVFQSLVNHLLVNQEVYERMMEEIDTASRKG

LIPETIPQYDAIQEHLPYYVACVRETLRLNPPAPNIFPRYVSEPGIELFGKFAPAGTEVTGNPWIMHRDT

ALFGEDAEEFRPERWLDPEKAKLYNKYIFTFGYGTRVCLGRDIAMEELFKGPLQPSARFEVKGGIGYWRD

MWVSINRRPAVKPL

#54

>CYP577A4 BAE58995.1 [Aspergillus oryzae]

MTITLVAIATGIVAFILYYICWSLFSSPLARIPGPKSFALTKWRLAYEDYKGTRTRKIHALHERYGPVVR

IGPNEVAFNSLSALRAIYGAGSGFERTNFYSMFAIYGRKNMFSFNTVKQHAERKKLFAHAYAKSAMLKGI

NASMIETKVRKYMEFLEREGRSQDIFTTLHYFSLDNITEFLYGDFGKTACLDGIEEDRALLHDILATGSR

TLSWLTVHHPKLTAWLYSRTGFLGCVARQFYPMQRPTPYTSIKLHATKAFQGFADASVAEKAKQSSLISK

LWKHHRSMKDGGLDNLDIASECADQLDGGIDTTSDTLMFTIWSLSRPEHWGFQQKLIDEVRSISEHDLNP

EGIPRVEAADKLPYVEAVIKETLRLFAPLPASEPRSSPEATTIDGYLIPARTVVSISPYTLHRNAEVFKN

PLEFNPDRWLDPSQDLTEMNRFFWAFSSGGRMCIGMHLAMAEMTTFVAALYRKYTTEPRGGFGTISPGIT

SRFEVFYDEGCSDMRVCFP

#15 62% to 578B1

>CYP578B3 BAE66519.1 [Aspergillus oryzae]

MTSATAIAIGILLAAFVYKYLVHPYCLSPISSVPNAHFTSPISNRWIEKQRNAGKEVLTIYNLHQKLGPV

VRLGPDELSVNSLSGLKTIYTGAFEKHSFYSDVFINFQTPNLVGMIHNNPHARQKRMLSRIYSKSYLQES

RDLRDISKIILSQRLFPILRRVAKSGEAINVLPLFQAVGMDFTSSYLFGTKNSTTYIFHLPEWQQWLEEY

EKFKYMSVQDRYMGFIESWCLSLCRKVENNDQPNDVPIATNAVVYNQLRQSLEKDPDSRPLELAIASEVL

DHLVAGHETSGITFTYMMWELSQHPELQAELRRELLTLTPNLRSLPISDNAVDLPSLPSPSAIDALPLLD

AVLRETLRLHSPAPAPLPRVTPASPTGVSIEGYHNLPAGVRVSSSSYSLHRIEEVYPQSSDWLPERWLKP

EPGKIHDMRRLFWPFGSGGRMCLGSNFALQEIKLVMAAVYTNFTTSIVDDEGIEQDHAFISLPTGRKLML

RFTPIKEA

#55

>CYP578C2 AP007155 Aspergillus oryzae RIB40

71% to 578C1, NOT IN GENBANK PROTEIN SECTION

MAGSPIVAISAIVLTITLLYKCIVYPVFLSPLSKIPNAHWTAPISPAWMLWKRFRSQNNR

TIQAAHERLGPIVRLSPSEISINCVEGGIKTVYTGGFEKHEWYPRVFGSLG (2)

TVSMFTMISSKPHSVRKRMLSNIYSKSYLQSSPHMRLISESILFDRLLPIIQEAVSSD

APIEVHDLNQGLTIDFVSAYLFGLANGTNWLQDAPFRRRMLHYYQGRKPYEFYHQEVPNL

VSWTKSLGIRLIPRWCDEANEMLDAWCLDLCDKAEQCLQSTEVSVEPVVYKQLQQAMIKQ

SSQKENTDESLKQQRLDIACELYDQLTAGFETSAVALTYLFWELSRHPDVQEELRA

ELLTLEPKILFPRSSASRGLPQAKAVDSLPLLEAIVTETLRLHAPIPGIQPRVTPYPSC

TLAGYSDIPANIRVNAQAYSLHRNPDVYPDPETWQPKRWLKGVNSDSDLEERRRWFWAFG

SGGRMCVGSNLALQ (1)

EIKLVTAAIYSNYRTSIVDDDNIEPIDAYTVKPRGDKLVLKFEAA*

>CYP578C4 AAJN01000108.1 Aspergillus terreus NIH2624

58% to 578C1, 34% to #158

MAVSAFTVVALVSLGLFVLYRVIIHPAFI

17000 SPLSKIPNAHWTAPVSPVWILWKRYTSQNNRTIQAAHEKHGPIVRLSPNEISINCVENGI 16821

16820 KSVYTGGFEKHDWYPLGFGSL (1)

GHRTVSMFTMTGSKPHSTRKRM 16641

16640 MSNIYSKSFLQSSPHMRLISENIVFSRLLPIFQDAASSGKHLEMHDINQGVTMDFVSAYL 16461

16460 FGLANGTRFLQDEAYRKKMLHLYHSRKPFEFFNQEVPNLLSWTKALGIRLIPRWCDDA 16287

16286 NDILDRWNLELCDKAEASVGSTELDAEPVVYKQLKQAMLKHTSKEDNQNS 16137

16136 VPDTPEKQRIEIACELYDQLTAGFETSAVALTYLFWELSQHPELQTKLREELLTLDPSIR 15957

15956 YPATSKILPSAKSVDSLPLLDAIVTETLRLHAPIPGIQPRVTPYPSCNLAGYTGIP 15789

15788 ANTRVNAQAYSLHRNPEVFPEPETWQPERWLKDGNSSAELEERKRWFWAFGSGGRMCVGS 15609

15608 NLALQ (1)

EIKLVIASVFTNYRTSIVDDDGIEAIDAYTVKPRSEKLVLKIEVA*

#158

>CYP578-un1 BAE55033.1 AP007150.1 [Aspergillus oryzae]

29% to 578C1, probable pseudogene, last exon may be wrong

MTQWIALLLVAICG

YLALRLRSSSLSKIPAAHWSARFSPIWILWKRYTGKELHTLVDAHQKHGSIVLVGPQD

LSVSCYQDGIRRVYDSGYPKPAPFYSMFNYYR ()

QQNAFTSLDRSEHGLRRRRTAALYSKSAL

1613568 MQSQHLRDITRSITYDRLLPKLNSVAQGNGRIDGLDLSYCICVDYLSSFIFGYSNGTNYLSQPKSAIDVW

RFHYENLMCQESFFVQETPSLYKLLRYISIDLLPRKYTESADFLGRWMSDMASKADRATDRKRSTGLPLA

LEDEPVVYDMAKEAVRKDSPHLSEGDQRKQVASEMFDHIC (?)

Missing I-helix motif here, 7aa

LVLGYAFWYLAQHPDAQQRIQTELNSQGID

MRSRETVTNSSKRPRAVELDSLPYLRAVIDECLRMRPTSTPLPRITPSNRKVSVAGIDGIPPGTRINTFQ 1614560

WFVHRDPQKWDNAHDWNPDRWLTRGNTDNKNEREDVLWAFAS

GPRMCLGNNWTYY (1?)

LTTLNSTAMQHILATICSSFNFTALPREENQCWPGSPEDELPIRVCLRN*

#18 79% TO 584E2 55% to 584G1, 73% TO 584E1

>CYP584E4 BAE62566.1 [Aspergillus oryzae] SHORT

MISSING N-TERM

MIMNSSHYPVALLAGAPARTTVNRYIFTTDGDHWAQSRAMIRPNFVKDQVAHLDIFEELMADLLALIPTD

GTTVDLQDLFFCYTIDSATEFLFGHSVQSLKKRLSGVKLDDNDFASSFNYAQDAIAKNTRLGPLRHFFRD

TKAEHCNQVCHELVEQFVEKALKYRANYDEEKAATDDDKKQRYLFLQGLAQQTGDPKRIRDELMNVLLAG

RDTTASLLSNMFFMLAKNPHIWNKLREEVASLEGRAPTYEQLRNLTYLKYCMNESLRLHPVVPSNARFAI

NDTVLPVGGGPDGNAPVFVPKGSIVAYSVYSMHRREDFYGPDANEYRPERWADLRPSWEYLPFNGGPRIC

VGQQYALTEAGYVTVRLAQQFSVLESRDPGPWEENLTLTLCSRNGTKVALRH

#17 60% to 584E4, 51% TO 584E1

>CYP584E5 BAE65988.1 [Aspergillus oryzae]

MELLPKLVILLLAALGIASVSIRRFLNRRQFARRCGCQPVARSFSKDPFLGLDTIPGTIRAIRQHRVLGR

SCEIFRAYGNTFTVKELHQSAIVTIEPENIQAVLSLNFKDYTLRHRLELFMPLLGRGIFNTDGQHWASSR

ALIRPSFAREQVANLSLLERLMQDLFVLLPRDSTTVDLQELFFRYTIDSATDLLFGQSVGALKKSQSGLA

FADALQYALKAIPVRDMLGPLNAVYRDRKTDECNRICRDFVQQYVEEAVYAAGPKKEEKESRTTETKRRY

ILSHELASRTSDKQRMVDELINVLLAGRDTTGSLLGNLFFMLAKNPVIWAKLRAEVAVLQNRPPTYEELR

GLRYVQCCVNESLRLHPVVPTNKRKAMRDTVLPRGGGNDGLSPVFVPAGTLVGYNIYAMHRRTDFYGPDA

KEFRPERWEDGKLQPRWGYLPFNGGPRICLGQRYALTEASYVLVRMAQEFRGLESRDPGSWEEGLVLTVC

PRNGTKVGLIP

#19 55% to CYP584E4, 49% TO 52H1, 44% TO 584B1

47% TO 584E1

48% 584E2, 49% TO 584E2

>CYP584G1 BAE59590.1 [Aspergillus oryzae]

MAVSLSFLTYLFLIVAGVYLARKISRAVQIRQFKKRHGCLPPPRDHHKDPVLGLDEVRSMLRVFREDYLM

EYTLEKYRRHGNTFATSVLGDDDIFTAEPENIKTILAVKFKQFDLGETRRRTFHPLLGDGIFAADGPQWE

HSRTLLRPSFTRTQIAATDLHERHIQRLISRIPRDGSTVDLQELFFNLTLDTATEFLFGESVESLRLGSS

AGSSSFAHHFNVAQDEIAFSMVIAPFDQLIFRPRFRESVREARGYVGNFVKKAIEYRHSLDAEKHAGDTT

DSQSRYVFLEELAKETDNPSDITDQILNILLAGRDTTASLLSMVFYNLARRPDIWDLLRSEVATLDGKCP

SFEELKQLKYLSWVINETLRLYPVVPSNSRTANEDTFLPVGGGPDGKSPVFVAKGQRVAYDVYVMHRRHD

IFGPDAEEFRPERWETIRPGWGYLPFNGGPRICLGQQFALTEASYTTVRIVQSFKEITSRDPEPYRERLA

LTLASRHGTKVAMVPA

#148

46% to 595A1

>CYP595B1 BAE58469.1 [Aspergillus oryzae]

MFGQIFSSVFAWTLIAILAGLYRFTTKQRPIFPVVNDYRGDFFRRKAYREYNQNAKKLIVDGLAKHGSPI

TLRVPDGLKIVLPSALSEWVKTNRDLDHQELIREEYFAGFPGFEAQDTLHAPDGMLIKLLRTNLSQNEEI

VPTVNRHIGPALQHYWGDSGIWHTIDWEDDTTGIISRAAASIFVGPEKAADDEWQTVVQAYVREYFAAVS

ELHTWRASLRPIVQWFLPHASACRRLLHQSRAIMQEVVRKREREAQAAEDQGLVAPRYNDVLAWTMQVPD

NKHPAGDIQLALAMAALFTTTELFKQILINIARHPELVEPLRKEIKTSLLGHGLGLTALAKMELLDSVMK

ESQRQIPVTVGLERKVIRDTSLPDGTKLPKGSHIMVDATDMWNPEVHVNPEVFDGYRFLKRRHAGDKASQ

FVQSSREHIVFGGGRHICPGRFFAGTELKLCLAHILLKYDIRLKEGYYPQPMVLGVYAIVDPMTQLEVRR

REHTEDLVF

#145

72% to 613C2, 51% to 613A1, 50% to 613D1, 39% to 613B2

>CYP613C1 BAE56604.1 AP007154.1 [Aspergillus oryzae]

MLWILVAVVVIVGISSPFTRRITIEILSTILD (0)

90386 RYLRSRFPIFSVDGSRTLPTCPYKWPNGQGDVAKFLQGIENRDLWEKEHGQ

IYRIWSGMKSEVVLTQPSHLQAVFRDSNKHSKAENNNSGYL MSELLGQCVGLVSRERWRTLRAVTEIPFQ

HDKMPSYLELIQRHTRHHFDRLLASGDLRQERIHPAQDLKMLPFWVVAEIFYGECDAEMKTELQQLCVLR

EDLFKRMIQGGIVRWQWSKYLPTATNRALAEFQRRWRAFNQRAYDRACQQQRILPIVLMIEAAREGSTSV

EQIYQTIDEALFANLDVTTGGISWNLVFFAAHSDIQERVRQEVLSATDHDAYLLSSSTLLAACISESARL

KPLAAFTVPQSAPTDRIIGGYNIPAGTNLVVDTYALNIRNGFWGADSQCYRPDRFLEHRATELRYQYWRF

GFGPRQCMGRYVADLVIRTLLAHLVAHYELGWVEPDPGKNSTWQRDLESWITIPDLQLRCVQRRND

>CYP613C2 AAKD02000041.1 Aspergillus clavatus NRRL 1

note: a (-) strand sequence 72% to 613C1 is upstream of the pseudogene CYP-un3

49% to 613A1

MPWIILALPVLVLVTLLPRKVTNSIFSAIID (0)

24668 KYLRWRYPIRSIDGTHTLPTCPYQWPNGQGDVGKFLQGIENRDAWEKEHG

KIYRIWSGMKPEV (2)

VLTQPHHVQSVFRDSNKHSKAENNNSGYLMSELLGQCVGLISQEPWRHLRAVAEVPFQHD

KAPAYVQLVLRHIRQHFSSLHVHGDLQQGRIHPAQDLKMLPFWIVAEIFYGEIDIAMREE

LQQLCLIREQLFRRMIQGGLVRWRWSRYVPSATNRELTDFKSRFRRFNQQAYQHACNQNK

WSLPIVSMTEAAQSGKVSLEQIYQTIDEALFANLDVTTGGISWNLVFLAAHPEIQNRVRD

EAQTATACEAYLLSSSTLLAACVLESARLKPLAAFTVAQSAPTDRTVEGYCIPAGTNIVV

DTYALNIHNEFWGTDSQSYRPDRFLKHRATELRYQYWRFGFGPCQCMGRYVADLVIRALL

AHLVENYTMGILDGDEDKWKRDSESWITLPDMQLKCEPRDVPMTKL* 23208

#144 50% TO SEQ 145

47% to 613A1, 48% to 613C2

>CYP613D1 BAE58578.1 AP007155.1 [Aspergillus oryzae] short at N-term

MGFSIQHGLTKPEILYPFLFGIFAVASLCIATLLFPASFSAASRVISWVLS (0)

4127460 IYLELKNPIRHTETGRNIPGPSYVWPNGQGDIEKYVQGRSRSEQWQRKYGNVYRIWAGMTPEV 4127272

4127213 VLTRPEQLHAVFKDSDKHTKATNSDSGYF

MSRILGQCLGLMAGPRWKLLKGTAAPPFMHPTAVRSIGRIQEHVRAHFHDLETNGNLREGRIHPVQDLKM

LPFFIVAEANYGSLTPAMKSELDSLAPARENLMKFVLFGGLARFNISRFFPTEANRQLRRFRSQWRAFNR

AAYERAREKHPSAMVVQMYDAVHKGVLTEEQVAQTMDETLYANLDVTTGGLSWNLVFLAANPACQARLHE

EISALTPAEEEGYISRNGTYLAACVLESSRLRPALPFTIPQSAPTERVVDGYRIPAGTNYVVDTWGLNVR

DEFWAPDNSTYRPERFLNSSNTDLRYHFWRFGFGPRQCIGRYTADVVIRAILLHLVKHYELQMLEEGDFT

QDPECWITHPDLQVKPN

#98 53% to 617D1, 63% to 617D2, 54% to 617D3

>CYP617D4 BAE57429.1 [Aspergillus oryzae]

MMGLPWATVYIVIAGYAIAKSTFGNFILLSILVSLLKVFYNWVLYPDFFTPIKKIPSPPGRSWITGNSDT

VFLEAPYEHMRKWLENVPNDGLIRYYVSINLERILPVGPRALKEILVTKSYDFPKPEFIRASLKRLAGEH

GLLLVEGDDHKKSKKNLLPAFAYRHVKEMYPIFWSKSIEMVRVMDEDLRKKADPTDNVLRMGAYASRAAL

DIVGVAGMDHDFQSLRDPNNKLVRTYQNLMSEPPLYMKIIFLLTLLLGDPAVVHDLPLERNRSIERSSET

IRDVARQMIRQKRAKWESGSSTAEDIDIVSVALRSGNFTEEELVDQMMTFLGAGHETTSTALQWCVYVLC

KHPDVQTRLREEIRANLPPISTENPQPPAATDIDNLPYLNAVCNEVLRYHPSVPATIRCASRDTTIIGEP

IPKGTLFLIAPEIIGKSKELWGPDADKFNPERWLGPGRANNGGADSNYANLTFLHGPRSCIGQGFAKAEL

ACMVAVVVGKYHMELKNPNAPLEIRQQATVCPKDGVLAKFTNIEGW

#92 52% TO SEQ 30, 48% to 620E2, 46% to 620E1

>CYP620G1 BAE59625.1 [Aspergillus oryzae]

MMYTLSTLVFLAGILGLYVLLNSKAPRAPLPPGPKGLPIIGSVGSDLPRGGRDWEHWLKHKELYGPISSI

TTAGHTHIILNDANVATELLEKRSARYSSRPRLVMANELSGSDIFVTTQNEHAIVRALRKRILSQLRSEE

VLLSFYPQVDTLIRRFLLRTLQKPEELIGNIKTGIGGVILKVVYGYTVEFHDRDPLVDLVGETAVAFGRI

NQPTGYLVDSIPAPLSVKYLPSWFPGAGFKKEAREYRRGFDTLLNWPFTFARRQMEEGNYEPSFVSRLIE

QRGSLLSLEEEVKIKHAAAAVYQAGYDTTASTITSFFLAMALFPAAQHKAQEEIDRVVGARLPTPEDRGK

LPYVNALINEVLRWNPVAQIGIMHAATEDDIYEGYLIPKGAPIVPNIWAIAHDPDVYSDSMSFKPERFLA

SDGHTPERDPHTLVFGFGRRICPGRPLTDFNNFLTIARSLAVFQVQKATKDGKEIDPIVDYQGGIIGHLS

PFEVSIRPRSAEHEALIRSIEVEDSISRGDSAALESVRV

#29 69% to seq 31, 53% to 620E2, 49% to 620E3,

48% to 620G1

>CYP620H1 BAE60302.1 [Aspergillus oryzae]

MMLVVSLLLGLTGLYIIRWTLGERKTLKRLPPGPTSKPVIGNLLDLPSPGTPDWLHWLKHKELYGPISSV

TIFGQTIVILNDRQTVIDLMEKRSGLHSSRPQLPIAEITDWDDTLGLIPYNSRFRAYRKALHQEMGTPAS

ILKYHDIIDMETHRLLFRILENPEDLVQHIRKEAGSIILRVGYGYVTEPHARDPLVDLVDKAMEDFSQLV

LPGAWLVNFIPMLKYLPSWFPGNGWQETAKAYKKRVTAMRDVPYTFVQRQIKKQNHVPSYVSSLLEQGNV

EPGSEEEIVAKWSAQSLYGGGAETSVSSLACFFQAMVLNPNVQKRAQEEIDRVVGTSRLPDMSDRENLPY

INAVVKEVLRWHPVTPLGVTHAASEDDTYNGYFIPKGSILVSNIWAIAHDPELYHDATEFKPERFLGVNG

RTPEYDPHLLSFGFGRRICPGQHLAAANLYLAIARSLAVFDITHLVKNGKEVPVTPEFTTGIISHPAPFE

LSIRVRSPEHEKLIRAVEKSYPWEKSHAEELQLKI

#31 69% to seq 29, 49% to 620E2

>CYP620H2 BAE61267.1 [Aspergillus oryzae]

MLVLVSLVLCLTGFCLLQWALKERKIVKGLPPGPRPKPIIGNLLDLPPPGALDWLHWLKHKELYGPISSV

TIFGQTIIIINGHRVANELMEKRSGVHSSRPHVPIAELAGWQYTLGFIPYDSRLRAYRRALHQEMGNATS

ISKYHNILDMETHRLLFRILKTPDCLMQHLRKEAGSIILRITYGYITEPEAYDPLIDLVDKAMEDFAQVI

LPGGWLVNFIPMLKYLPSWFPGCDWQRRAKAFKQRAKAMTDIPYAFVKQQHEQQKHIPSYVSRLLEQNNI

KLGSEEELVVKWSAQSIYGGGAETSVSVFACFFQVMALHLNVQKKAQEEIDRVVGASRLPDLSDCKNLPY

INAVVKEVLRWHPVAPMGVAHASSKEDIYHRYVIPKGAILVPNIWAMAHDPDFYHNAMDFEPERFLKSGR

NEQNPEYDPHQFIFGFGRRTCPGQHLVSANLSLGVARVLAVFNITNAVRDGKKVPISPEFSPGVISRPAP

FELSIQVRNAECKRLIEAVGMKFPWEESHAEALAQLRI

#30 55% to seq 29, 56% to 620E2

>CYP620H3 BAE55186.1 [Aspergillus oryzae]

MPLPTLGSVLLGLAFLYFVKLVLLRKKTLAPLPPGPKPKPIIGNLRDLPRPGQQEWTHWLKFKELYGPIS

SVSMFGQTIVILNDHQAAFDLMEKRSAIYSSRPRLVFAAEIVGWEDVITLQGYTNRFRSYRKAMHRVLGT

KELMSRFNPLQDVEVRRFLLRILQKPDELIQHIKTEVGAVILKIAYGYNIEPHGRDPLIDLVNDSMENFS

AVVKPGTWLVDVIPLLKYLPTWFPGAGFKRTGYEWRKTLLATIEKPYQLVKQHMRQGSYPPSYLARLLEQ

IDGEPTAEEELVSKWTAGALYAGGADTVTPTASSLSSFFLAMALYPEVQRKAQKEIDRVVGPNKLPTFED

RDTLPYIDAMVKETLRWHPVGPMGVAHLVTEDDIYEGYLIPKGALILPNIWGFTHDPKIYRDPETFRPER

FLGDNPELDPHTLAFGFGRRICPGRLLADATIFLTIAQSLTVFNFSKPEGEGDLKAEFLPGVISHPAPYR

LEITPRSAAHEALIRSVEVEHPWEESHAKELEKVEC

#28 50% to 620E1, 53% to 620H1, 56% to 620H3, 49% to 620H2

47% to 620G1

>CYP620H4 called CYP620E2 but changed BAE62129.1 [Aspergillus oryzae] revised

MASLHLASIAIGVLTAYLLTKLLTFKKPPAPLPPGPPPKPIIGN

LKDLPQNGERDWEHWLKHKELY (1)

GPISSITVLGQTFIILNDQKLAVELLEQRSKWHSDRPKMFFAAEM (2)

SGCGGILGLIPYSDRSRAIRKAMN

KEIGSKVAVSRFNALQEAETRRFLLRVLEAPEELRNHIRTEAGAVVLKLAYGYTVEPH

KQDPLVDLADVSMYYFSLVCRYGAWVVDVFPSLRFLPSWFPGTEFKRIGQRSKEAFDN

FGGKPYNFVKHQMSQGTHHPSYLSSILESEEIEPGSEKEYVTKWSAASIYAGGADTTV

STMASFFLAMALYPEAQRKAQEEIDRVVGNSRLPTFADRDNLPYINATVKEVLRWHPV

VPNNLPHLSTHDDMCQGYFIPKGSIVISNIWGFAHDPDVFHDPMTFKPERYLGDNPEP

DSHRISFGFGRRICPGRVMADAAIYLNIAQSLAAFNIGKKVVDGKEVEPRVEFQAALI

SHPEPYDVSIKPRSSVHEELIRAVEEEYPWEKSHADELVNIKV

>CYP620H4 AP007164.1b Aspergillus oryzae CYP64 like

formerly CYP620E2

second P450 of six on this accession

seq assembly modified at cyan region

CDS complement(join(1111497..1111884,1111943..1112214,

1112271..1112515,1112568..1112719,1112780..1112971,1113193..1113425))

53% to CYP620e3

MASLHLASIAIGVLTAYLLTKLLTFKKPPAPLPPGPPPKPIIGN

LKDLPQNGERDWEHWLKHKELY (1)

GPISSITVLGQTFIILNDQKLAVELLEQRSKWHSDRPKMFFAAEM (2)

SGCGGILGLIPYSDRSRAIRKAMN

KEIGSKVAVSRFNALQEAETRRFLLRVLEAPEELRNHIRTEAGAVVLKLAYGYTVEPH

KQDPLVDLADVSMYYFSLVCRYGAWVVDVFPSLRFLPSWFPGTEFKRIGQRSKEAFDN

FGGKPYNFVKHQMSQGTHHPSYLSSILESEEIEPGSEKEYVTKWSAASIYAGGADTTV

STMASFFLAMALYPEAQRKAQEEIDRVVGNSRLPTFADRDNLPYINATVKEVLRWHPV

VPNNLPHLSTHDDMCQGYFIPKGSIVISNIWGFAHDPDVFHDPMTFKPERYLGDNPEP

DSHRISFGFGRRICPGRVMADAAIYLNIAQSLAAFNIGKKVVDGKEVEPRVEFQAALI

SHPEPYDVSIKPRSSVHEELIRAVEEEYPWEKSHADELVNIKV

#81 48% to 620E2, 46% to CYP620H3

>CYP620J1 BAE60640.1 [Aspergillus oryzae]

MSYMVLGLAIGVIFLYFIRSFLARTKSFAPLPPGPRPKPIIGNLWDLPPQGTRDWLHWLKHKDLYGTYSQ

HRHIATTDRLRKNSGKAQLAPSPSWRSVPPYTHLDRRAPLPICKHRLFTTYTAGYGDIMTILEYSERLRT

TRKVAHQQIGSNKAISRFSHIQDAEVCRYLLRMLRDPGNWLEHIKKQVETGAVILKITYGYTVEPHGRDP

LVDLAEDAVGKFSLAMVPGAWLVDSIPICExxxxxxxxxxxxxxxxx

SSFYSVMEHVEAVRGQQKQMTQGSNVPSIISYYLESENIQ

PGSEEEHLVKWATATLYGGGADTTVSTMMCFFLTMALYPHVQRKAQEEIDRVVGATRLPGFEDRGNLPYI

DALLKEALRWHPIVPMGVAHMAMEDDMLEGYRIPKGAAILSNIWCVRPRTPYFLLLTKLPCRAFTHDPNE

YHDPMTFKPERFLSDNGHTPERDPHLLAFGFGRRVCPGRNLADSNLWLTIARTLAAFNIAKPIRDGKEVD

IQPEFQAGLISHPEPFDVDIKLRSAGHHELILAGEKQYPWEESHAEELRRAIAVL

#27 51% to 620E2, 51% to 620H3, 43% to 620E1

>CYP620K1 BAE64937.1 AP007171.1 [Aspergillus oryzae]

14 P450 genes and 2 pseudogenes on this contig

MESILLFLTISLVGLYLVGSHLSKKHSLGHLPPGPPRKPIIGNLTDLPSHDVCDWEYWLKHKDLYVYSAT

TSSSSMTPASHETYSRSALPSGPPDPVGTLSRAGWNKILGTLAYSDPSFKDMRKAIGHQIGSKTAASRFN

AVQDLEVRRFLLRVLEDPDNLLQHIRKVAREAGAIVLKIAYGYTIEPHGHDPLVDLADEAMATFGLAILP

GTWVVDFIPILKHVPTWFPGAQFARMAKQFRKSAAAFSDVPYAFVKRRLAQRDFEPSFLAGLIRKNEEQP

GPGSYEETVIKWAAASFYGGGSDTTVSTMSSFFLVMAQYPHVQRKAQAGIDCVVGPDRLPSFQDRENLPY

IYAMVKEILRWHPVLPMGTAHASVMDDTYEGYTFPKGTLMVPNVCWVVQSLGDNSAFTHDPSTYPDPHTF

KPERFLSYEGHEPEANPYYLVFGFGRRVCPGRTLADANLYISIAQSVAAFTITKPIRDGKEVDLRAEYQS

GAISHPVPYNVTITPRSPRYEELIRAVETEHPWEKSHSEELRLV

#139

47% to 628A1 Fg, 45% to 628B1

>CYP628C1 BAE61392.1 [Aspergillus oryzae]

MDTLWVLAFSSGVATHLLLYRSGEWDIKAPSIVKIYTLLGATLVYLERADLL

DGFPVSTRPKWGIAVILYHIFGVYAS

MLFYRAFWHRLCGFPGPFLARLSNFYVTSLSAKRLHLYEEVQKLHQQYGDYVRLGPTELS

IADPQAVKALYSGQAKVTKGPWYTVLEPRVSLQMSRDKKEHARRRKVWDQGFSSK (1) 891591

891531 ALRDYEPRVSHYARQLLEAVR

KNVGKPMDMAKW

FNYYSFDVMGDLSFGKSFNMLAGGQDTYFSTQLHADMKSIGLFSHLTWLFPFFKRIPILNKDYLKFWDWV

GGRVEERIK (0)

891143 NEPDRPDVFSWILDAFQNGPKTKQDHLDLHGDAYLIIVAGS (2) 891021

890959 DTTAATLTNLFFHLAADHTWQAKLQEELDALPDLTQEKVTNVKLLDALINETLRLH

PAVPSGTQRLTPPEGLQIGDKYIPGDVMVCIPTHTLFRDERAFVRPDEFLPQRWMTQPELVKDASVFIPF

NAGPYSCVGKQLALMELRRVTAEILTRYDVEFAQGQTTEDFLDGKRDTFTLVTAPLKLVFRER

#56

>CYP630A3 BAE62007.1 [Aspergillus oryzae]

MPGIYVLAAAGALALGVGYVVILPIIHYFYDPKGFRKYPNFAPLAGITDLPYCYLSSCGYRSKDLYEAHK

NAPILRIGPNNLSFGRIGAVKDIYGHNTPCIKDIKYAMTWGSHTHLFDVIDKADHAAKRKRMSSAFAIKN

SERWEHKVANVTGRLVKALDAHCTLPLLPGQTEPQAADVTLDYNKWINLFTIEAINLIALSSTLGLLEKG

SDEVTAQRKDGTTYPARYRKSQDSTAHAQSLFVWDYKYFHWLSRLSKLVPKYRQMWKDGEPWGDVIYHQA

VTRLQRYQSGEKLDDFFSSLMEDKAGHPNNLEWGEIVAEVGAIINAGADTTAIALTQVLDILIRHPKYLQ

RLREEVDSTLDADEVVAPYDKVKNLPFLRACLDEALRLIPPTSAGLPRRTPPEGAQILNEWIPGDTSVSM

TSYSAHRDPEIFPVPEEYNPDRWMDLDNRKRMEPYFVPFSTGARGCLGRNITYLEQTVVLATLVHRYDFA

VPANWKLGRFEAFNLIMGEMPMKIWRREKA

#13 59% to 630B2

>CYP630B11 BAE66547.1 [Aspergillus oryzae]

MYSLLLIVTLHFLLIYYIIIPIINYFRDPKGLRKYPNLTFVSGISDLPLIYYSHKGIRSRVLFEAHKKHP

VLRIGPNKLSYADPVAIKDIYGHGTKCTKDVFYSALSGSHYHLADVVDKEDHARKRKVLSNAYAIKNLEG

WEYKVADMTQRIIKAFDERCTSPLPKGRNPDPQDLTIDYRMWTNLYTIAAIANIGLSEDIRFLDQGNDII

SSEAKDGTVKKVHFRDCLYANASATSTLVWAYDWYEALVRVSKLVSSTYRQKWKLAEDWDGIVNNRATTR

WKRYEKGEKLDDFFSALMEDKAGAPNNLEWGEIVAEVSIMMNAGSDTTGISLNNVMLLLLKNPHCLEKLR

EEIDGVLEDDEVIAPYDKVKHLPYLRACLDENMRMYPPVSFHVPRRTPQEGTMIRGEFVAGNTSVGISAY

VVHRNEDIFPDPDTYKPERWLGDKGRDLQPYFVAFSAGARGCIGRNISYLEQTVLLASLVHRFEFALPSP

SWEPVRHETTNFNSGPMPLKVWRRASRVYEDN

#14 57% to seq 13, 75% TO SEQ 69, 77% to 630B2

>CYP630B12 BAE65338.1 AP007171.1 [Aspergillus oryzae]

14 P450 genes and 2 pseudogenes on this contig

MWSALSIAPYALVLGLFSLLYFVVFPWVEYIRDPKGLRKYPNMNPFSGMSAVPFMLLASRGFRSKELQEL

HRTKPVIRTGPNMLSYGDVRAIKDIYGHNTKCIKDPSYIVTAGTHYHLADVVDKPDHARKRKVLSSAYAL

KNLETWEHKVSDKVEKVVAHFDKVCTAPPSAAVAAGKMAPDPKDLTVDFRAWTNFFTLDAIADIGLSEKL

GFLDSGSDVCIAERKDGSTYEVNLREALYPTARKQSLILWNYEWYPVLNKMVNIIPFFNRMQNSSDNWDN

IVWRRSMNRLRRYEAGEKLEDFFQAMMEDKNGRANNLEWGEIVAEMNIMMNAGSVTTAIAIANVMYQLLR

NPQSLKKLQEEIDAVLDADEIVAPYDKVKHLPYLRACLDESLRIFPPTSHGLPRETPPEGMEILGEWVPG

NTSVSMSAYVAHRDESVFPKADQYIPERWLGEEGKALQPYLIAFSAGARSCIGRNISYLEQTKILATLVH

RYDFALPYPGWELKRLETMNLILGDMPVKVWRRNVQEA

#69 75% TO SEQ 14, 62% to 630B7

>CYP630B13 BAE63103.1 [Aspergillus oryzae]

MLSLVLVAPYAAVGGLCTLLYFFVFPFIEYIRDPKGLRKYPNLHPISGMSVLPFMFMASRGARSQELSEL

HKKSPVIRTGPNTLSYGDVRAIKDIYGHNTKASKDPSYIVSAGTHYHLADVVDRADHARKRKVLSSAYAL

KNLETWEYKVSDKIERLVKHLDKCCTAAPVSGGRAFVPSSEDLTVDIRAWINFFTLDAMADIGLSEKLGF

LDKGNDICVAERKDGSTFECGLRDALYPLAIKQCMVLWNYEWFPIINKLVDVFPYFRNLQKKGDAWEHII

WRRSSERLRRYEAGEKLNDFFQALMEDKNGRPNNLEFGEIAAEVNIMMNAGTVTTAIAITNVLYQLIRHP

EAMAKLREEIDGVLGPDEIVASYDTVKHLPYLRACLDESLRILPPTPHGLPRQTPPEGMEILGEWVPGNT

LVSISAYVAHHDESVFPQPHKFIPERFLGEAGKELGPYFITFSAGARSCIGRNISYLEQTKALATLVHRY

DFALPYPDWEPKRFESMNHILGEMPIKIWRRSFDG

#82 43% to 630B2, 48% to 630C1, 44% to 630B5, 43% to 630A3

>CYP630D1 BAE60571.1 [Aspergillus oryzae]

MALEKLFIVVFLCFALRPLLLYFYDSKKLRKYPNQNFLSGVTNLASIRERQRKFRTRELYLQHQKHPIIR

VAPNMLSFRDVKAIKDIYGFGSPCQKHEIYKLQNSEGHMNILNVIDREDHNRKRRMLSHAFSTKNLESWE

FKITDKVEKLVAQFDRRAHPPAWKNEPSQQNNTTVDVRYWLNLFTVDAIADIALSERLGMLESGSDVVKV

GGPGEEDSAHRFIEDMHEAARVKSKIIGTLDWYYVLKQVSSFLSSRCRSQWDCGGNVGQIVEHLAGKRLR

RHEDGENIDDFLSCLINDKAGKSRNLDIGELKAETSILLDAGSETTAIALTHLLYYLIKNPDCFVKLRKE

VSGAIAGDKVAPYAKVKSLPYLKACIEESLRLSPPLPRGLERVTPAAGAYIMGEFIPGNVGVSVPAYVAH

RDPDLFPEPEAFLPERWFNNENIGKMRDAFIPFSAGGRACIGRNITMIEQQILIATLVHRYDFSLASPDW

TLQNEEAFNLWPVELPVKIWERDLEA

#74

39% TO 630D1, 37% to 630B1

>CYP630D2P BAE62696.1 [Aspergillus oryzae]

AP007166.1 Aspergillus oryzae RIB40

Downstream from CYP551A2 (BAE62699.1) (145541-147583) note: two other genes between these

This gene is a pseudogene missing the top half

136724 VQTTEYYRSLQEHGKDGCFDNTIFDSVLRSFAEIQEGSNLTKVQTEKLVDEAFAI

MFAGSDTTAFSLTITLVYLCKYPEKIVKLRREVQCLRDFKVQDIQLATISQMPYLDAVIREANRLSSPLS

TVLPREVPSTGCVISGHFLPKGTVVGFHLDDINRNPKFFPEPNDFIPERWSGEEGKKLQRWFVPFSKGSR

RCIGMDFAFVEMKLAVAAIISRFEIWLDNPNVTLNSREMFVKIPEDDLRIRLRAITV

135844

>CYP630D2P AAIH01000541.1 Aspergillus flavus NRRL3357 pseudogene

same gene as Aspergillus oryzae BAE62696.1, 97% identical

runs off the end

418 VQTTEYSRSLQEHGKDGCFDSTIFDSVLRSFAEIQEGSNLKVQTENLVDEAFAI

MFAGSDTTALSLTITLVYLCKYPEKMVKLRREVQCLRDFKVQDIQLATISQMPYL (0)

DAVIREANRLSSPLSTVLPREVPSTGCVISGHFLPKG (0)

TVVGFHLDDINRNPKFFPEPNDFIPERWSGEEGKKLQRWFVPFSKGSRRCIGMDFAF

VEMKLAVAAIISRFEIWLDNPNVTLNSREMFVKIPEDDLRIRLRAITV* 1301

#23 52% to 631B1, 43% to 631A1

>CYP631B2 BAE65443.1 [Aspergillus oryzae]

MRRALHVAQVRGLATGQCRPRVQASRSIIRPREEPFRSSVMLILLGLLCLYTGLYVARTYWRLRHFPGPL

VARFTDLGRLWWVKTSRSHHHHMGLHSRYGQYVRLGPNMISISDPDAIPLVYPIRPGVPKSDFYRSMMPY

TRKGRSLPLVFNTRDEDLHKRLKTPIAHLYSLSNILTFEAFVDQVLEILFRQFEERFVPDQAPFNLGNWL

QYFAFDVMGTMSFSRRYGFLEKGRDDTGLLSAIWAFMKAAAPVTQMPWVDLVWNKNPFIALFRATPAQPI

LNVVLSRINDRRNELYSTTSTPEKVNERDFLSRFMHIQSNSDTIPPWAVTAWSFSNVIAGSDTTAVAMKT

LWY

NLLLHPATMHRLRKELVQAQQQSKLSHPFPAWNEISGLPYLNACVNEALRIHPPFCLPFERIVPAEGMTIGD

HFFPGGTVIGMNPWVINRHRPTFGEDADAWRPERWLEDPARTRQMEN

TLLSFGAGRRVCLGKNIALLELKKLTSALVLHYE

LEIVNPEKFQSQNFFFFKQEGLYAAVKRRSAGSPELYPDDAVPH

#156 52% TO SEQ 23, 56% to 631B1

pseudogene

>CYP631B3P BAE56559.1 AP007152.1 [Aspergillus oryzae]

24632 NLLTHPATLQALSSELITANLTLPYPKWNEVCDLPYLDACIQEAVRLHPPF

ALVLGRVVPAGGVTVLN

HYLPEGTLVGGNPYVVNRHAETFGPDVEEWRPERWLEGEGRKRLEQS (0) 24285

FDAGRRVCLGKYIGILELKRLVPFLVLKYD (0)

MKIIDPERFSVENGFFFKQRGFYCNITRRKEGSDRDKADPK*

#138 46% to 653A1, 51% to 653A2,

>CYP653B1 BAE61541.1 [Aspergillus oryzae]

MDYMDYLKEMGLAWHWVPIFVGLLAFYFIMERSTANASRTSLPIVKHFKLLPPFFNRILYVVKAPFLIYY

GYEKYKSKPFRILKLDGDLVVLPQKYLEEVGGLHSRQASLVGAKYKNILGGCTNILINSELPARTVSEKL

NPVLDRQIPRLLFELHHAFSAVVPYCEGRYVPINLYHMILKLVTHSTSRIIIGQRLCRSEQWINTITKCT

YDVRIAVKQLQLVPRFLRRLVAPFLPSVQRLETQLRWIAEQLILPMIQHRRRRELNDPSYKKQEDFLQWM

MDLADNDLDRDPMNLAYGLMITMALAVVQTSTMLITHAMYDLMVHPEYLEPLREEIHETLTNGWIRASLS

DFGAQRRLDSFLHESQRLNPPSEVSAQRVLGRPLTLSDGVTLPKGTHVCFPSGPMSRDPTVVPDPLTFDG

FRWCKDLNAPDGSLTDVSPANLHFGFGGQACHGRFFGAVIAKAVMSRLLAEYDLKFEEGQSGRPENIVNG

EQIMPSISTKVLIKKKNVDI

#117 40% to 653A1 45% to 653A2, 41% to 653B1

63% to CYP653C2 from an indole-diterpene gene cluster

note the other P450 in this cluster is CYP698A1

The ortholog of 698A1/paxQ (698A2) is 4 genes upstream of this gene

>CYP653C1 BAE65194.1 AP007171.1 [Aspergillus oryzae]

14 P450 genes and 2 pseudogenes on this contig

1897240 MDKLTATLAKVNYPSEVENGSMLLVVTLVILFLWFIIPSPVKRSNVSVPTVTLFNPYLPEFLSRVWFNST

AATVIYKGYRQHKDRAFRLLKPDGDIIVLSNKYVEELRQLPLTTLNALEAVFEDHVGKYTTILNDSHLHT

EVIQKRLTPAISRFIPRIIDELDHGFAVEMPECEDKWALIRPYEVFLRLVARAGARVFVGPEICRTEKWL

TASIDFTKNIFMTITLLRPIPSFLHPIIGPMLPSSRSLDTQLRYVQDELLGPEIVKRRQRQASGDPDYEK

PDDFLQWMIDLAQNDKEGDPGNIAHRLLGLTSMAVVHTSAMSITHGLYDLITMSQWLEPLRQEIQEAMPD

WKSSSYSSLVSLRRLDSFLKESQRFNPPGELSFHRVVKKDLVFSDGLRLPKGTHICMASGPIGMDTKYVS

DPTTFDAFRYVDGDKAQSQFVHTSATSMHFGLGRYACPGRFFATFVLKAILSRFLVEYEFRFGPDQVGRP

KNMLLGDKIVPNTSVDVYVRKRTGSRSTA 1899076

>CYP653C2 Penicillium paxilli

GenEMBL AF279808

paxP gene

part of an indole-diterpene gene cluster, also includes CYP698A1

MDLSDFHISTPLRYFHEEASLLWKLGVFAVLVYFLLPKPTYKTN

VKVPTVKYMGPWMPEILSRIFFNSHAPTVIYKGYEKFKTSAFKVVKPDGDLVVLSTRY

AEELRQMPSTTLNALEATFTDHVGGYTTILTDSHLHTETIQKKLTPAIGRLIPRMISE

LDHAFEVEFPTCDDQFASINPYTVFLRLVARVGARIFIGDELCREEKWLQASIDYTKN

IFLTIALMRPMPGFLHPIVGRILPSSRSLKDQLSYIQQDLLGPVIKERRRLEASSDSE

YKKPDDFLQWMMDLAQNENESHPDNLSHRLLGITSMAVVHTSAMSMTHILYDLLTMPD

LIEPLRDEIRNEIKDWNKATQADLSRLIIMDSFLKESQRLNPPGDLSFHRVVKKDLTL

SDGLFLPKGTHICMAAGPISKDPDVVSDPDTFDAFRFVKQRTATSGFVSTGPNNMHFG

LGRYACPGRFFAAFVIKLILSRFLMDYDFKFETEHKERPKNLLIGDKIVPNVATPILI

KRRATKA

#122

40% to 654A1 just upstream of #121

>CYP654B1 BAE64653.1 [Aspergillus oryzae]

286826 MDLFPRDYLFAGLAVFIFWWIIDHSRHKQRYIRNVPIVGGKANLKRNRQKFVTNSLQILRTGYEE (0) 287020

287082 NNDELFYVPTPIGERLIIPGRYLEELKSADMAVVDFQATFLE (0) 287207

MFEGAYTTLGTHSRLLPQVVRAQLNQYLPDVLPEIQFKVALQIHREIPFNAITECFAKSDWTVINVTELM

AVLVARVSSRMFGGPALSQNREWIEASLRFAHDGFNAAQKLKMWPDTLKFIGQHFIPEVRSIKNTYKIAE

RAIIPLLDEREVDKSKKAHDLLTWMYDQAQGAEKDKKFIAGTLLKVSFAAYHTSAAAPTQLLFDIAAMPE

HIAPLLGEYLSAPRDNNQNISVKGFAQMVKLDSIMKESQRFNPLLLLTFERIIKRDFTLSDGVVIPANTW

IGCAAQAIGMDRKLYPDPDTFDAFRFVAKEEATATSTSVPATKAHYTSANPGSMAFGYGQHACPGRFFAM

MEIKAIIGEILSRFEMRLADGEMRPPSVTFETQHLPHPAGKVLFKRRRCT

>CYP654B1 AAIH01000140.1 Aspergillus flavus NRRL3357

99% to CYP654B1 Aspergillus oryzae

MDLFPRDYLFAGLAVFIFWWIIDHSRHKQRYIRNVPIVGGKANLK

RNRQEFVTNSLQILRTGYEE (0)

NNGELFYVPTPIGERLIIPGRYLEELKSADMAVVDFQATFLE (0)

4806 MFEGAYTTLGTHSRLLPQVVRAQLNQYL (2)

PDVLPEIQ (1)

FKVALQIHREIPFNAITECFAKSDWTVINVTELMAVLVARVSSRMFGGPAL 5162

5163 SQNREWIEASLRFAHDGFNAAQKLKMWPDTLKFIGQHFIPEVRSIKNTYKLAERAIIPLL 5342

5343 DEREVDKSKKAHDLLTWMYDQAQGAEKDKKFIAGTLLKVSFAAYHTSAAAPTQLLFDIAA 5522

5523 MPEHIAPLLEEYLSAPRDNNQNISVKGFAQMVKLDSIMKESQRFNPLLL (1) 5669

5722 LTFERIIKRDFTLSDGVVIPANTWIGCAAQAIGMDRKLYPDPDTFDAFRFVAKEEATAT 5898

5899 STSVPATKAHYTSANPGSMAFGYGQHACPGRFFAMMEIKAIIGEILSRFEMRLADGEMRP 6078

6079 PSVTFETQHLPHPAGKVLFKRRRRT 6153

#119 60% to 655A1 partial C-term seq

>CYP655A2P BAE65179.1 AP007171.1 [Aspergillus oryzae]

pseudogene

14 P450 genes and 2 pseudogenes on this contig

1863432 QGDVVQVNNNVMHRD

KDVWGEDADEFKSERWFGLRPYWDFVPHSGGPRRYPAQLLVTTEASYVVARFCQRSKAVENRDVNGYIPI

MRAGPVDSNGVKIAVTPV* 1863121

#152 49% to 655A1 C-term is wrong

>CYP655B1 BAE56825.1 AP007154.1 [Aspergillus oryzae]

MNNAISEPRITPSLRPSPKMQAPTLTSASAEGSDTTAHGCGGCTHFACQPIIMFTLTEYFIAACVWLVLY

KVGNLLWNRHH

YFKQQKARGCGEIKHYRHRDPILGLDFVYTLSKAFKEHRWLPWQQELFAAQGVKTFQAN

FLGSRAIYTSESENMKAMSTTYWREFGLEPLRRGSGAADPVAGPGVSTVDGPMWDFSRNIIKPYFTRDGY

SNLARLEVFVNRLLDLVPTDGSTFDMQPLLQRWFLDTSSEFLFGKTVDSLTHPENVKVAKAMVDTMRGIR

VRLTMSKLMFLHRDPVWMENVKIVRDFVDERIDASLTQLQDVKSGKGTSCTENQPDGRTDLLWDMVQQLQ

DKEALRGQIMAVFIPSNDTTSILISNAIYALARHPHVYQTLREEVLALGDQEITFEKLRG

717492 LRYLRYVINE (1)

717406 THRLYPNGIQMVRIALEDTTLPVGGGPDQSQPIF

717304 IQKGDIVHANRYLMHRDPDNWGPDAEVFRPERWGDVRPLWKFVPFGGGPRICPAHVLVDT 717125

717124 EASYVLLRFVQRFRTLEPRDERPYKAIMRIGPSNLHGVNVAVKTA* 716987

#79 52% to 657A1, 60% to 657B1

>CYP657B2 BAE61082.1 [Aspergillus oryzae]

MGKDGLSTDGLIQNAWSGSSELVWPLLATVLVLCCIATRIISGFQSRVDSKTEQPQSVKTLPYWFPWLGH

SLSFVWDHVSFTEKSRDYMNASVFGSYMGGTKHNTIVSPSMIKSIMQSKATTSAPLVNRALKAFGDDLGS

LRDLNTTDYQVFHHKLPNLLMREPFITEASETTIQLLKREVPNFVTFCRSVVDQTLWERGSDVEVVDDNG

DKPACEANLFDLVRGFIGNITTTTLMGQAILEAFPSLLDDLWVLDNRFPLLAIGVPRWAPLPGVPAAYAA

RDRILNSLAAYQQAFLQWDDGIDPGVKFRDLEDVSEPLKQRIRTSKNLGLSPRSSAPGHLSLLWAMNVNS

SNIAFWYLVRLYNDPTLLEEIRKEISPYVKAHRPSREETGFPFEEPPRISLNLKGLLDSCPLLKASFYET

LRLDSADMSFRKLTSDLTITESNEDVTNSDRTKPHSYKVYKGESLILHHGVLQNDSRCFSNPSQFDPLRF

IITDPETGEKKANMHTIHPFGGGMSGCKGRTFAERQLLAFTAAMIVMWDIEPMDGSHFTVPRHRQSSGAY

LPKNDIRGKTLWNVNNHGVVVFIRGGRHYLAPPMADRDLSFCITCRIYIVQNIHI

#131 61% TO SEQ 132, 40% to 660A1, 47% to 660B1

>CYP660C1 BAE63219.1 [Aspergillus oryzae]

MSDILSIIGLTLLVAFLTSVVFFVQRRKLDPREPPLASSAIPLVGHLASFLYYGLEYFAIARCVTRTIPS

LDGVEMLTAKLVSAVRRSRNAMSFGPLFANVAENGGGINGRGMQLLRDKEYGGQGVGQQTADSMHPALLG

SGLDQMNGKMIAVLKTIIDELASQPDNVVDLYEWCSHAVTVASTDAVYGPLNPYRSESNRRAFWAI ESNL

SLLMMNVVPWITARKPWKGREQLTQAFIQYYQADGHLDSSQLAYTRWKVQHEAGAAIEDIARLEALTALG

ILSNTVPTCFYFLFDIFSRPDLLGKIRDEILDGAFSVDSAGVHTLDLADIRERCPIFVSTFQETLRTRSN

SGQLRVVQKDTLLDDHLLVKAGSIILMPAAVINKHPSVWGADAGTYDPERFSKIDPAQKRSKASGFMSFG

SSPHICPGRHFASGEILALVAMILVRFDVRPVRGTWVEPKGNTKAVAASLPPAVEKVEVKFSETSKFAGV

KWEFRLTPGKGTFGLITG

#132 61% TO SEQ 131, 38% to 660A1, 56% to 660B1

>CYP660C2 BAE56717.1 AP007154.1 [Aspergillus oryzae]

382760 MSGSTTIVGLSVVAILASLLFTQRAKLDPREPPLVSSTIPLVGHLISFLIYGIGYFAT 382587

382586 ER (2)

VYIIASPDLLPSVRQNRSTMSF 382413

382412 NPLFTAMAQRAGGIQKPGLQLLREEELGGQGLAKKTVEVMRPALLGNKLDHLNEQMIHAL 382233

382232 KHIVHQVASSPTLPFDLYEWCSDALTVASTDAIYGPLNPYKSEAIRKAFW (2)

DFESNLSLLLVDTLPWLTCRKAWKGREQLVQAFIQFYQADGHLSASSLAYSRWKAQQEAGASLEDIA

RLEILTGIGILSNTVPSCFWLLFDILSRPELLSAIQDEIHQNALSIDSTGTHTLDLADIRGKCPTLLSSF

QETLRTRSNSGQVRVIYQDTLLNDRWLLKAGSTLLIPAPSINKNNSTWGSDAGDFDSQRFTKIAHQTHKK

SKASGFLSFGLSPHICAGRHFATGEILALVALLLVRYDIRPIQGSWTEPKTNAKAVAASLPPAAEKFMVT

AVERPEYKGLEWRTTVTPGKGTYGLIIGLDIYLQLSSVGEEMRRG

#57

>AP007151.1 Aspergillus oryzae RIB40 genomic DNA, SC005

CYP666A2

50% to CYP666A1 Aspergillus nidulans

join(3144893..3145283,3145344..3145394,3145416..3146254,

3146318..3146493,3146532..3146865)

model wrong, frameshift at 3146521

3144893 MLGIILFSLFISYTAYLGLCFLRHWSLARKIGLPYVSFPISSHN

ILFLSLFETRFVPYVINTWLSPKLADFIYGSAFKTRWAARDRLHRRYGGVYMLITPSV

STCMVCDASVASQICMSRHGFPKPIKQY (1) 3145283

GALEMYGPNIVT (0)

3145445 SEGSQWAHLRRHTATPFNERNSALVWEETIRQTKEMVQYWEDEYSRSSSASEFILTDTRED

ILKFTLNIICSVGYGVKLPFRPGLENSTESAEGLFKDAITPLPGYHFTFRSAMEYLNK

HITSMFIANGLLPKGIPRSVLPFFKKDFDAFDDIGRYLRALVSTAETKETLSQNLIDG

LVRSKQKIDKDQGLDPELTEDEILGNLFVFTIAGHETTAVSLRFALVLLALNQDAQEY

LYEGIREATYDEPRNPVEWDYRRVYPKLVSPLC (0)

LETLRMYPPVSGIPRWTGDSAVN

ITYHNQPYLLPPHVYVNVNASGLHYSEEYWGPDAAV FDPKRWDKR 3146521

3146521 NTRSFLAKNKEGGLSGPGLEYDTIHK

PVRGSYIPFSDGFRSCIGKKFAQVEFVVAMAIIFREYRVMLAKSNERETEDDLRRRAE

KALGESTAFITLSMRDEVPLLFQKRCVSKA* 3146865

>CYP666A2 BAE56143.1 [Aspergillus oryzae] assembly errors

MLGIILFSLFISYTAYLGLCFLRHWSLARKIGLPYVSFPISSHNILFLSLFETRFVPYVINTWLSPKLAD

FIYGSAFKTRWAARDRLHRRYGGVYMLITPSVSTCMVCDASVASQICMSRHGFPKPIKQYGALEMYGPNI

VTVRCPNVFRVSANVSQSEGSQWAHLRRHTATPFNERNSALVWEETIRQTKEMVQYWEDEYSRSSSASEF

ILTDTREDILKFTLNIICSVGYGVKLPFRPGLENSTESAEGLFKDAITPLPGYHFTFRSAMEYLNKHITS

MFIANGLLPKGIPRSVLPFFKKDFDAFDDIGRYLRALVSTAETKETLSQNLIDGLVRSKQKIDKDQGLDP

ELTEDEILGNLFVFTIAGHETTAVSLRFALVLLALNQDAQEYLYEGIREATYDEPRNPVEWDYRRVYPKL

VSPLCVMLETLRMYPPVSGIPRWTGDSAVNITYHNQPYLLPPHVYVNVNASGLHYSEEYWGPDAAVFLAK

NKEGGLSGPGLEYDTIHKPVRGSYIPFSDGFRSCIGKKFAQVEFVVAMAIIFREYRVMLAKSNERETEDD

LRRRAEKALGESTAFITLSMRDEVPLLFQKRCVSKA

#58

>AP007157.1f revised Aspergillus oryzae RIB40 genomic DNA, SC023

CYP671B2

join(618168..618393,618483..618641,618711..619251,

619318..619721,619797..620050)

sixth P450 out of 8 on this accession, one stop codon

55% to CYP671B1

MDFISFLHQENFKEVLETDLLQLGVS

AALLGVLLHITIFRTSFCVENHIYNLLGLYATVVLFLFYAYFTITVLSL

MQALGRVALLTTLFNTSLNTSISIYRLFVHRLHPFPGPFACKLT

RFYSAFLAAKNIQYNVELKRLHKQYGDFVRT (1)

GPR*ISILRNTALPLSYGPQTGCRRSTWYGHVDVDDTK

ICMALSHDFNDHRRRRRAWDRAFSIISLSVYEPRVIAQAKKLMAQVEANQGKPLDATT

WSMLFTFDIMGDIGFGKNFGNLTTGKAHPAISAIRDNMRVIAVVSHLPWLLNMLGKIP

GAAAGYQGFFKWCTDQVETKRK (0)

SWDHDEYPQDII

SWILKAFIDNDVSAPPSEPALHDDSRVVVIAGSDTTALALASIIYFLAKHPQILQKLQ

AELDNAMPNGPRSWTYDKAKTICYIDDIIHESLRLRPSVSGGGYRVTPAEGLQIDEVF

IPGDVNVFVPQQLIQTDERYYKFSKEFIPERWGEKKVEWGTDKAPYFLFSLGIYGCVG

KNLAMLSLRVAVSTLAQRYDIRFRTGDILERCIGYIHHFSAAFASCVSPKEIVNTTPF

LRLLQVFFILHISGVKVRT

>CYP671B2 BAE58815.1 [Aspergillus oryzae] assembly errors

MQALGRVALLTTLFNTSLNTSISIYRLFVHRLHPFPGPFACKLTRFYSAFLAAKNIQYNVELKRLHKQYG

DFVRTALPLSYGPQTGCRRSTWYGHVDVDDTKICMALSHDFNDHRRRRRAWDRAFSIISLSVYEPRVIAQ

AKKLMAQVEANQGKPLDATTWSMLFTFDIMGDIGFGKNFGNLTTGKAHPAISAIRDNMRVIAVVSHLPWL

LNMLGKIPGAAAGYQGFFKWCTDQVETKRKVLSPRYAFGLAVDETSVYPDATQQSWDHDEYPQDIISWIL

KAFIDNDVSAPPSEPALHDDSRVVVIAGSDTTALALASIIYFLAKHPQILQKLQAELDNAMPNGPRSWTY

DKAKTICYIDDIIHESLRLRPSVSGGGYRVTPAEGLQIDEVFIPGDVNVFVPQQLIQTDERYYKFSKEFI

PERWGEKKVEWGTDKAPYFLFSLGIYGCVGKNLAMLSLRVAVSTLAQRYDIRFRTGDILERCIGYIHHFS

AAFASCVSPKEIVNTTPFLRLLQVFFILHISGVKVRT

#77 43% to 673B1, 37% to 673A1

>CYP673C1 BAE61747.1 [Aspergillus oryzae]

MTLLQNIVSETPLAAGSALLTAIIVAYVIYQRYLHPLAKYPGPFLASITDLWQVHQFLSLQQPYNLTALH

ERYGPIVRYGPDKLSITHESAVPTIYQKSAKSMPKTEFYDAYGAAHPNVFGMRDEVMHSVRRRHMSHSFS

LSYIKEMEEYLDLNIRILKDKIRYHSEIGEVFALKKALHYYMIDVLGELAFSRSFGVQEADDESRIPPVI

EHSLLAAVTGAWPTMTMTLKRWLPYMPHAGLRRLFAGRKACADLASSSVQRRLRDLNDGESSVGVQNRKD

ILTNLIKAKHPETGERLTQTDLETEAFGFIIAGTHTTSATSTLLFYHLLHNPDFMRKCTEEIDSNLPSLG

PSETAYSITAAEASLPFLRNCIRENFRITPVFTMPLARRITDPAGVTIEGEHLPQGTSVAVCNHAFHHNP

VVWGEDHNIFNPNRWNDPNVGAKARLLMHFGLGGRQCIGKAVATTNIYKLLSTLLKEFTFELADEQERVD

VDKGLYKGRIPKLFSVGISDLQGPLLVRARVR

#106 63% to 675A1

>CYP675A2 BAE55129.1 [Aspergillus oryzae]

MDLLRLRDRSLLGQGALVAFVTYWAVWIIYTRWFHPLAKFPGPFWASVTRAWTLLHVLHGDAEKRQMKLH

AKYGLVPPGDILEPYLSSIGSVVRIAPNELVTSEPEAIQTLYGARSFTAKATLGYVRTQYYPQQRLIGVA

RFPDHFSSEGGKQHGERRRIVSHVYTMTSILQSEKYIEKCIAVWLEKLGQMADRKESFDLWIWTRMYAYD

VIGELYFSKMFGFLQAGHDHLGYIDATEDLVPIQFLAANMPTYVRGLFMLTGILFPKVRRALRALGDLTD

ATNAMLKDRLAAIQSDSEDKPQRHDILGKLLEISQKRGKELDFVLDDIKMESFGAFFAGSETTALTLSGI

LYHILRNRSVYEKLTAEIDTAVQCNQLSTPHISYNDAIKLPYLTACIREGIRMHPITGVSFPRHAPSSGC

TIGGYYIPPNARIGVNPGVMHFNKTVFGEDADQFRPDRWIDGDVSKMDRYIMQFGMGARTCLGKNISMCE

IYKAIPELLKFYTFELAGDEDMQTTSYWLYKPVAIDVNVRRR

#121 56% to 682B1

>CYP682B2 BAE64654.1 [Aspergillus oryzae]

MTLISLSLLALSLWIIIRVLVIIYRLAWHPLARFPGPKFAAATSAYEFYFDAIKGGQYTFEIGHMHKKYG

PIVRISPHELHINDPGFIEELYPGPGKPRDKYAYATGQFGIPDVCSLVLTPYDLSSVFGAVSHDLHRMRR

GALSPFFSKAAVTKLEPVIYSAVDKLISRIEEVVESTGFVDLTMAFSCMTTDIVTQYAFAESSRFLENPD

FTPNFHEAILAGTRMGSWARHFPILFPVLRSIPIDILSRMSPETGVFLRWQESMRKKVSEIWQDQSALPV

KDKNVSPFGSTIFHELFHSDMPDSEKHPGRMWQEGQIVIGAGTETTAWTLTATTFFILDNPNILSKLRKE

LAATMPNRYEKPSCRELEALPYLIIIQEGLRLSFGVATRLQRINSEAPMIFRQKKTNDTIEEKVWEIPTG

TPVGMTAALVHLNPELFPDPHEFRPERWLDQDGQLHRGLDKYILSFSRGSRQCIGINLAYSELYMGIGIL

IRRLGDRMQLFETDRTDVDMVEDCFVPVPRRESNGVRVRLSAQ

#105 68% to 682D1

>CYP682D2 BAE55187.1 [Aspergillus oryzae]

MVHPSVLVEALVMFRPIAQFLLFPPFCMFQFLFQPWIIALAGAGYLISLAVYRLWLSPLAKFPGPKLAAL

TLWYELYYDIYCEGQYTFQIIRMHEKYGPIVRISPWELHISDPDYYEVLYSRDSPRNKYEYYTRQFGLTK

TAMATVDHYHHRLLRSNMNPYFAMTRIRKLEPLIQGLVDKLCDRLREFKGTGTPVALQYPFTCFATDVVT

DYTMGAGFHYLDEPDFVPRWSRTLSGVAKSGVYIKPFPWLIKVFNALPESWLSWLNPEMDLTFHFQRRCR

EVIASIMEEQNANGYDKVKSQFSHPTFFHDVLNSNLPPEEKSPERLWQEVQVVVGAGAETTGKALTWTMF

YLLHSPDKLQKLREELNQLDPDRTATLLDFEKMPYLTSVILEGLRLSYGLSTRLQRVAPDRALQFREWSI

PAGTPVGMSSTLMHHDERIFPDSHKFIPERWLDLEQRKHLEKYMVAFTKGSRQCIGMNLARSEILLALPK

VLRELDFELYETTLEDVTLAHDMFLPFPKMDSKGVRVLIK

#59

>CYP684A2 BAE62328.1 [Aspergillus oryzae]

MDSGFIRDNIPAIASIAVLVYVLSVVYRSQRSHLSYLPGPWYTKFTDLPLRYKVVTGQRPRYVHALHDKY

GPVVRIGPDEVDVSDISGAREIHRIGSGFLKSPWYSLLNRKDTQSIFTTTDPKFHNAHRRLLSSPMSELS

LKSMEPLIDARVRLTIQKMQEEMKTRGVADVYKWWFFMATDIIGEITFGESFRMLEKGKKNQYVKDIEMI

SVIGGIRASFPILVKLATLLPLPIFKEVNASGNRVFGYATESINRYKRLLAENPENPKPTVFTKLYNAGE

EGLPDNEIRDDAQSFIVAGSDTTANTLTYLVWAVCRDPQIKKKLVDELAEIRDDFTDEDLRSLPYLNQVI

NEALRLYPAVPSALPRSVPPKGTTMGGHWIPGGSTVSTQLYSLHRDPVAYPDPEKFEPSRWASPTKLMKD

AFMPFGAGSRNCIGLHLAKIELRLATGYFFRYFPNAKLSSKYDFNDNDMEQMLFFLMSPKGKRCLLEV

#118

>CYP698A2 BAE65190.1 AP007171.1 [Aspergillus oryzae]

61% to CYP698A1 with some assembly errors

698A1 is from an indole-diterpene gene cluster

note the other P450 in this cluster is CYP653C2

The ortholog of 653C2/paxP (653C1) is 4 genes downstream of this gene

14 P450 genes and 2 pseudogenes on this contig

probable GC boundary after VTAE

frameshift after VETIL

1893711 MSRLLERTLDRFTGLLEYQPTYPFAAPTWVYLVGAILIQQLATRWYRYYKSW (0)

VNVPVVGGHGIIGSWIAAFWWTARARSLVNEGYQK (0)

HGDFAFQVSTPTRWEVFICNDEMVREYRNFTDERFSANAVTAE ()

LFEAKYTVPGAAEGVHKVPVPIVAKALTWQRTRAATKTDPYFEEFVKELQHAFDAETKFENED

WNDLCCFATGTRIVAHLTAKSLVGYPLSRDTELINLFAEYGNAVPTSGFFIAMFPQILKP (2)

FAAKFCSAPKISARLDRIVMDELRKREANPRSEPQ (0)

DITDWIMFWSRTYPGTYTDQDIARSVVSAVFGAIHTTTQ (0)

VLVHCLMDLAIRPEYIHPLREEVETIL

IGDNQQWTKEGLESMEKLDSFVKECQRFNPLDA (1)

GSLARRATKDFTFSKGLHIPE

GTFVFTPNSPVLFDEKHYPDAQQFDGYRFYRLGRVTGRPLEYKFIAANLKYLQFGDGRHIC (2)

PGRFMAADEIRLLLAHILVNYDIRPKDDGERPPNWTF

KKILFPDMKGMVQLKRRSINISQPN* 1891669

>CYP698A1 Penicillium paxilli

GenEMBL AF279808 note TG boundary at intron 1 not AG

PaxQ gene

MDFVLSALQRDSWGIAAIILVSIWALHSFHRSRKLQ (0)

IPVPYVGKCGILGPWISALQWESKARELVQEGYEK ()

HGNFAFKVALLNRWEVCICNEDMIREYKNLMDNQFSAIAVTSE ()

LFQIKWTAPGTEEGAHKISIPLLGKALTWQRNRSAAQNDPYFSEFV

EEFLYAWKEEVPVPENGDYELPCFETGARVVAHLTARSLVGYPLCRNPEIVNLFTDYG

SAVPTSGFFIAMFPEIMKP

FVANFCSAPRISKRLQAILLEEFAKR

REEGGIEST ()

DIMGWLRNWTDQNEPGVYGDLEITSSIIATIFGAIHTTTQ ()

VLVHCLFELATRPEYVEPLRVEIQSA

LEEHGGWVKEGIEGMVKLDSFIKECQRFNPLDAG

SLARRATKDFTFKNGLTIPE ()

GTFVFAPNGPILFDDTLYPEARQFDGYRFYNLGQKTGKPQDFRFAATNQKYLQFGDGRH ()

TCPGRWMASDEIRLMLAHILMNYDIATKDNKGRPENWIFKKILFPDMKAVVILKARK

SVSA

#60

>CYP5042A2 BAE62162.1 [Aspergillus oryzae]

MGASIHAHISAAANESPITAAFVSIAACLGLIFLIIQEIFKPVGKRRSPQGKKWKLPPGPQGVPIFGSLL

YLQKVREDEEHRIHKDLAKYGEMTTLHLGSKTWILLNSKRVVSEIIAKRGSLTNGRSPMPIASGIVSRDG

RSLLLPPAQWMEKRRVMHSLLSGTAMKQYGSWQELESTQMLAEYLFQPERWYRHHYRYANSVVHRIALGE

RLVKSGKELAELQDVVTHFVGSIGTSLVDWFPELDKLPRMLQPWRKHWEKLGDWNEEVYKSWWIPAREKV

ENGTAPPSWVRDVLLHPDTKFTGNDQEAMYVALQLLEAGSDTTREALNIFAMAALCFPDKFQKAREEVDS

QCSTTKNFRLPGIDDLGNMPYICAMIKELLRWRPIFSFTPDHVLTSDMEFEGYNFPAGVGFVINEIPVCN

ECEDPEDFKPERWLDGHETDAAHGLWQFGGGRRICVGYRLAFQGLFINVARLVFCYNYEAAGPYDSKRLN

HHKTVEPFPVKVTPRSEQHTSLILEEGARLGVLEDAKRLI

#61

>AP007157.1h Aspergillus oryzae RIB40 genomic DNA, SC023

CYP5042B1

complement(join(1170838..1170975,1171028..1172102,

1172148..1172220,1172254..1172488))

revised to match to 5042A1 and 5042A2 but stop codon encountered

MTQLSIETFVVFTAFGLLWLSYEYFRPGSILSRFASMRSKLPPG

PPGLPIFGNMFQFTRARDAGLWGPFVSPGFLPSAASLRQYGPMTTLHMGFQTWVVLNGP

*VATDIINKHGKITS

ERPDMPIAGDLVSHGLRTVIRPTAAWTEGRRVMHHLLSGSVLRIYGNWQEIESLQML

SAYLREPKHWYAHHYRYSIAVLYRLVMGENLSKTQDELNDYQKVTMEITLSSLNSMVD

FFPRLDRWIPIYLQWWRPYWAKMGDFHHSVFKSWWDPIRDAVRQGTANPGFVRDTLLH

PDMRYKGTEEEAMYLATSVIAAGSDNTRMALNTFIMAMISHPETMARARQQLDAVCIN

EDGTLRLPGMADFDRLPYLAAMVKEVLRWRPTTPVTPQHQLTEDLEYEGYRFPKGTCF

VVNGIALGQVCDNPDQFDPSRWLDGNEGNIVHNLWAFGGGRRICVGYRVAQQALYVAI

ARIIFCFDLCADGPIDTRKLNHFHIHNEPFPVRVTVRSAKHEFLIKEEAEKASTAF

>CYP5042B1 BAE59001.1 [Aspergillus oryzae] assembly errors

MTQLSIETFVVFTAFGLLWLSYEYFRPGSILSRFASMRSKLPPGPPGLPIFGNMFQFTRARDAGLWGPFV

SPGFLPSAASLRQYGPMTTLHMGFQTWVVLNGPERPDMPIAGDLVSHGLRTVIRPTAAWTEGRRVMHHLL

SGSVLRIYGNWQEIESLQMLSAYLREPKHWYAHHYRYSIAVLYRLVMGENLSKTQDELNDYQKVTMEITL

SSLNSMVDFFPRLDRWIPIYLQWWRPYWAKMGDFHHSVFKSWWDPIRDAVRQGTANPGFVRDTLLHPDMR

YKGTEEEAMYLATSVIAAGSDNTRMALNTFIMAMISHPETMARARQQLDAVCINEDGTLRLPGMADFDRL

PYLAAMVKEVLRWRPTTPVTPQHQLTEDLEYEGYRFPKGTCFVVNGIALGQVCDNPDQFDPSRWLDGNEG

NIVHNLWAFGGGRRICVGYRVAQQALYVAIARIIFCFDLCADGPIDTRKLNHFHIHNEPFPVRVTVRSAK

HEFLIKEEAEKASTAF

#32 45% to CYP5053A1, 44% to 5053A2, 39% tp 5053B1

>CYP5053C1 BAE64935.1 AP007171.1 [Aspergillus oryzae]

14 P450 genes and 2 pseudogenes on this contig

MVTFWTGRRPTIWICDAWAADELLNKRAAIYASRPRMVVFSELGAGQSNMVNMYYGDRWRLHRKLTHMGV

GLQQVRNYRGFQNDESKVVALDLLREPREYVSHFERYATSVVSIIGFGRRVSAYTDPIITEVVAVMQRAA

ELNVPGKSFPMLMESFPFLAKFPNWMAPWKHGLGNGQGRGRPFFYALAEEAASGPEAKQCYARKLFEEVP

KHNLTKMEISSLAGNLFGAGSDTSSSTLVTFVLACCAFPETLPKAWEELDRVVGPHRSPTFEDESDLPYV

RAFVKEVLRWRSVAIIGGQPHAPIKDDRYKVRCTSLNSVLGWLIPRNTWVQGNVWAIHHHEREFPEPDRF

NPERYFEESPVHRPFPVDKGYMTFGWGRRVCSGQGLAEQGTFITIARLLWGFDIRKALDRHGNEVPVDIF

DFTNGLNMRPNSFECRITPCNQEIRSTIEREGLQALQDLSQYDGETKYRMSTYYNPEKL

#151 73% to 5061B1, 45% to 68D1, 41% to 68L1

68% to 5061B4

>CYP5061B5 BAE57341.1 [Aspergillus oryzae]

MPVLATFLESHYFFQGIAVALALIFVSNFYRELADGLPYRKIPLVGRSRWEISNTKAKKLFVTSAKDLMV

QGFSQGRTVFQAMFTAGPTIVLHPRYVDELKNHPHLDFGEAVRKSFFGATIPGFEPFNNQTKEDIVIEVI

NKKLTHTLGQLTIPLSKETAAVLTDKLPGSDEWKPFTFAQEIPHMVARLSSLVFLGEKICRNETWLDVSV

NYTIDAFNAARELRDLPAVARPFIHWFMPSMQKLRHHRKVAAEIVQQEIIKRDMIREGKLPEENPPRTHA

DALDWFREVAAGRPCDETVSQIGLSVAAIHTTSNMLTNVMYDLTAHPEYIQPLRDEIKAIVEQDGILKKT

SLTKMKLMDSVMKESQRTNPVSIAFFNRIATEAVVLSDGTSIPKGANVVVSAHVMEDESIYPNAKVYDGF

RFYNKRQVPGNEHRYQFVTTSPEHLGFGHGMHACPGRFFANNEIKILLAHLLLKYDWKFADRVDRPKSFL

HGTEIICDPTVKLLYKSRQPEVDLSALGEGTTD

#62

>AP007159.1 Aspergillus oryzae RIB40 = Aspergillus flavus

CYP5064A2

join(1096818..1097070,1097124..1097466,1097500..1098547)

This gene seems to have an AT boundary instead of GT at intron 3

49% to CYP5064A1 partial seq.

1096818 MDFGGILLHLEARSMATVLIAGLLVYWVGSAIFLAVVHPLAKFP

GPKLAAASDWWLVYHEWFLGKSLTDILFDLHQNY (1) 1097052

GPVVRYGPYR (0)

LHFSSMQAYSDIYNVKSKWDKDPEVYKAIVDTSSAFGLRNYH

EAKERRDLIWPFYSRQSVQRMQKAINRQ (0)

1097494 ISFLVDQLDKQNQEGKLSNMS

MAFRCLAQDIMADICLGKSFGTLEERDFGSPLIHALDEGLESYVLMKSFPTLRNILYS

ISAMVTLPGEAEFATYGTIVADHVTRGIQQPDTIPPNTMLGFLVPTSSNTAKEAPQPK

LSQGHMTEELQTFVIGAGETVASAMVQGLSGILQSPDLYKNVYEEIVQVW PETDGPVP

PIEVLEKLPLLTAVIKEALRLTHGVVTPLARVISAGGACIDGHHVPGGTSVGTSHVFI

HMSSDYYDAPDEFRPERWLGSSSDKHLVAFSKGPRGCMGINLAWCQLYLVLATLVRTV

QMEYPADLNDIKIKWKDCFQPLYYGRPLQVRCTRAEGHS* 1098547

>CYP5064A2 BAE59846.1 [Aspergillus oryzae] assembly errors

MDFGGILLHLEARSMATVLIAGLLVYWVGSAIFLAVVHPLAKFPGPKLAAASDWWLVYHEWFLGKSLTDI

LFDLHQNYGTINVPYGGLQDLWLDMDRIAYVVQSLTWESALEANSLVKLHFSSMQAYSDIYNVKSKWDKD

PEVYKAIVDTSSAFGLRNYHEAKERRDLIWPFYSRQSVQRMQKAINRQIGYRELFRTRTFLVDQLDKQNQ

EGKLSNMSMAFRCLAQDIMADICLGKSFGTLEERDFGSPLIHALDEGLESYVLMKSFPTLRNILYSISAM

VTLPGEAEFATYGTIVADHVTRGIQQPDTIPPNTMLGFLVPTSSNTAKEAPQPKLSQGHMTEELQTFVIG

AGETVASAMVQGLSGILQSPDLYKNVYEEIVQVWPETDGPVPPIEVLEKLPLLTAVIKEALRLTHGVVTP

LARVISAGGACIDGHHVPGGTSVGTSHVFIHMSSDYYDAPDEFRPERWLGSSSDKHLVAFSKGPRGCMGI

NLAWCQLYLVLATLVRTVQMEYPADLNDIKIKWKDCFQPLYYGRPLQVRCTRAEGHS

#63

>CYP5068A2 BAE57974.1 [Aspergillus oryzae]

MELIIGLCLLAPLLFLYTPLRRFIGLNASELPLPPGPTLLSGPFPEKDIAKTFQKWNKKYGPIVSAKIGA

QQFIILGSRRAAQDLLERRASIYSSRPASKFLDKYLHKGLASAFMPYGAQWRLHRRLGSSLLSERASTAY

RQLQDFESKRLLHEFLSTNDFSEAFLSYTSDIMFTLVYGKGRGKDDSDHKMLYQINEMATFVLQKASFGT

ILLDLFPMLDWLPHCFLTWRKKAEELHYKTKEVYTECGNIALGGDCWNWSHEVSQRSEAKELPWEDVCYA

LGELYVAGIHTTKMVLEILIMVCVLHKEVKQKAQAELDSVVGEDRLPSPDDLEKLPYIRAIVSELLRWRP

ISPIGVPHAVIQDDEYMGYRIPAGATVVANQFGMNMDEATFDNPAAFNPDRYLNNPDLPVSAFGFGRRIC

PGHRLARGSLLIVTSRLLWAYDITSAQGDADLGDEYSPSSVKAVFQPRSVKHEQVIKKEWEESDKDEKRI

LERIRDRI

#71 39% to CYP5068A2, 41% to 5068A1

>CYP5068B1 BAE63024.1 [Aspergillus oryzae]

MVSYEWCLDSFNPFGITRNSLCFFFLVALCLCWSSSAWSSKQQRLPPGPPALPVIGNLHQMPRRNRWRAL

QRWHKLYGPIISLRLGQRIAISLGTHKVARELLELRGNNYSSRPRFVVAGDYVSEGLHSILLPYGNQWRI

HHRIQRELLTNHRTQAYRYLQDIESKQIVYDMLKSSDFVGHFRRYTSSVMFTLAYGKRLESPGRHEIAEA

SKITENISLAADQAKNMIVEVYPVLDYIPRCFAPWKRIGTRLHAQTVQFFEQQMLEGLRSPAWNWSKHII

ALNETKDLSNKEIVYILGALYEAGSETTATVLQIFVLASVLHPECIGHAQLELDESVGNDRMPTFEDMPR

LPYLNAFIKEVLRWRPIAPLGIPHAPSKIGEFMGYSIPEGATIFPNNWTLDLDDAVFRDPYAFKPERWLE

DPNLPLSTFGFGRRACPGKQMAENSLYIAIARLIWGYNFHHAYENGLRVELDPWDMKEGVVSPPATFSAV

LSVRSSVHQRLIELEWDSAEKDVNGILGQIESLMGSRAAAQKDSSA

#85 76% to 5069A1

>CYP5069A2 BAE60165.1 [Aspergillus oryzae]

MDLYKAVVLDLHLSPGLISAIHKPTVLVGIACALFFSQLLFWTAKYSHKKTKGLADLPGPSGWPLIGMGL

DLPVRPRELLNRWAAQYGDVFKVRVGWYNWVFFNSPDAVKEVFDRQAAVTSGKPPLPIAQDYCLRGDGVL

PMTYNAKWKRLHAFLKQLLSAKASAAFIPSQEFEIKQLLADLSHEAGKNSTDFYMHIRRMTFSIVMTSAY

GLRIPKWDCQEVRDVYGNMRMLSIILSPGMFWIDMRHWDNLKDRITAGNRPRLLRQGPHRGLEEETVSWL

ASAVPEAGPETTASALNGLIRYLAMFPDAQARAHDEVTRVLGDTRMATLADEPNMPYIRAVIKETLPTVP

HGDVHYRDHLIPKGTILLANLNTLHWDPERFPHPFHFKPERYLNHLHRSAVYAAGGDILARDNFTFGAGR

RICPGIHLAENGLFLAVANITWAYEFKLPLDDQGEEIPLDITDEGFMEGAIRVPKQYTVRILERNAARSR

LIRSEWDQAQQAGYVLRGSHVDVNGGVGGGSKTAAS

#64

>CYP5070A1 BAE58765.1 [Aspergillus oryzae]

MALFTFRLLPLVCTASIAIYVLSRFMHFETKSTGALTYSITFIIYLLYWLYIYPYHLSPLRHVPTVPGCP

LWGHVFEIFNAEIGAVQQKWHKTHGPIVRYFYPFGKEILSVVDNSALKHILVEASYNYEKTASNRKFLSR

LFGEGILTAGGKVHAQQRKALNPAFSISAIKALAPAFWDYSCSMSSYWEQDIKESSDDSVSLDISDWASR

ATLDIIVAVGFGAKIDTLHNSTAPLIEAFRTVFRFDAVAKLLAVLHILFPIVRYLPIKENREVDAAKRTL

FEFASGLIQEKEANINSTGNNILSQLVRGDRKPQAAGEDIFSRVICDQIATFLGVGQDTSATWLSWTLHL

LSKHQHMQVKLREEIRSHFPFLFRGATHEKIDFTEVDVDRLPYLNNVCRESLRFIPPIPFVSREAARDER

LGEYFIPKGTVIHIPINTIHRMPEYWGPNSNAFDPDRWNCLPASYTNNAFLPFTQGPRGCIGRKFADTEV

KTILCCLLSKFQFSPDPAVQDPEELKRYRIVQKSQYGIRLKVSKLDG

#21

>CYP5075A1 BAE65911.1 [Aspergillus oryzae]

MLLLLLETLALAYVAWSLVAMEINYRRACAMKIPLVRLCIDPQNLLWVILEPHLWPWLDRLPINWGNFGR

YSRRGWYFADRGESHRRYGPIWALVTPKEIYINVADSEAIHDIFQRRTDFIRPVEQYTVLEVYGPCISTA

NTTDWPRHRKVLATPFNESVMSFVWDESVEQTRQMIDIWASPDLDKISSVAKDTRTLSLNVLAAIGFRKS

YPFRSGNGNSHREQSDSVSYRDALQTVLDNAILLMIMPRRLLSLSFAPESWQQLAKAATDFKQHMVRMLD

EEVQALNEGKAGSGGLMTSFVRAMNLKQEDARSKGLTTDEIFGNIFVINFAGHDTTANTLSFGLLLLAAY

PEVQDWVAEELLLLTNTKGQYADLFPKLNRCRAVMLETLRLYPPIPSLPKWTNDQAQPLKVGDRTIMIPP

KVGINPSLLTMHIDPQHWEDPLEWKPSRWITPTKLVRAISIEDEQLITPVRCTYFPWSDGPQNCPGNKFS

QVEFVAVIASLFRHHRISPVANAGETFQQTRERVLATTRDVDLQLLLRMKDAERVHLKCRRV

#146

42% to CYP5075A1 missing C-term

>CYP5075B1 BAE58484.1 [Aspergillus oryzae]

MLLIIAVALLGWTLYSVFCLVGNIRRIQKIGIPYHVIPCSPVNPLWILLEPLIFFILGLLPFEFGRIKHY

GRRTWQFTDKAQSHMRMGDAWAIATPNEIFVYICDADAITDIIARRADFVRPIELFTLLNVFGPNVATTE

GADWQRHRKIVAAPFNESLNSFVWREALTQAQSMLTTRATAGPSGGLGTDTRTLALNVLAATGFKRSTRF

QSAQEAQSEDPRSYAQSLKTVMLNTFLIMLIPPSVLKFPIFPSWCRRAGEAVEDFKQHMLNMFNTEKTLL

DQGKPGTGTLMSSFVRESTVDPKSNKTVLTLDEILGNIYVINFAGHDTTAGSLTYVLFLLAAYPNIQEWI

AEEIRTVFPNPDRDTWDYKEAFPRLKRCLAVVVSTRPNPPL

#65

>AP007151.1 Aspergillus oryzae RIB40 genomic DNA, SC005

CYP5076C1 = old CYP5066A3

complement(join(1085860..1086053,1086107..1086208,

1086271..1086569,1086625..1086746,1086798..1087521,

1087687..1087712))

locus_tag="AO090005000422"

MDIKEKPTLYAFA

1087673 LGILIHILYFRIGEHHLYPARYIYGYFGSFIGVAAFLYLVEELPVHSASYRSLYL 1087509

1087508 IFTHLLGLYSSLVLYRLLYHPLRHFPGPFLSRVSVFWLSVQLRHKTLYRKLADLHNEYGD 1087329

1087328 FVRVGPSDLSIIHPKAVNTIYGFKSACTKSAWYDSSAPLRSLHSHRNRAAHDKHRRTWTP 1087149

1087148 GFTDRALHGYEKRIQVYRQKLINQIKSMEDSKPLNINTLFTWYGYDVMGDLAFGQSFDM 1086972

1086971 LVKSESHWAILMIHSMLKPMEYLMPIWFFRLLLSIPGTTKAFWKFNEYWGQLFKMRMA (0)1086798

TKQEIPDISACLLEPLKGRAPTPDEFNVLLGDASLIINAG (2) 1086627

1086568 DTTATTLTTIIYELARRPGEVQKLRTELVSCTTDPNGEYTQESLAILKHLNGVINETLRI 1086389

1086388 HSPVPSYIPRKTPPEGINIDGTHVPGNMNVSCPQWVIGR (2) 1086272

1086209 SESVYQNAQNFIPERWYLYPKMIKERSAFAPFTT (1) 1086108

1086054 GPYTCVGKPLALMNIRATIARLITTFDMELPPGDDGRALERSMREHFSI 1085908

YMAKDIQVHFQKRAI*

>CYP5076C1 BAE55487.1 [Aspergillus oryzae] assembly errors

MDIKEKPTLSLYLIFTHLLGLYSSLVLYRLLYHPLRHFPGPFLSRVSVFWLSVQLRHKTLYRKLADLHNE

YGDFVRVGPSDLSIIHPKAVNTIYGFKSACTKSAWYDSSAPLRSLHSHRNRAAHDKHRRTWTPGFTDRAL

HGYEKRIQVYRQKLINQIKSMEDSKPLNINTLFTWYGYDVMGDLAFGQSFDMLVKSESHWAILMIHSMLK

PMEYLMPIWFFRLLLSIPGTTKAFWKFNEYWGQLFKMRMATKQEIPDISACLLEPLKGRAPTPDEFNVLL

GDASLIINAGGDTTATTLTTIIYELARRPGEVQKLRTELVSCTTDPNGEYTQESLAILKHLNGVINETLR

IHSPVPSYIPRKTPPEGINIDGTHVPGNMNVSCPQWVIGRSESVYQNAQNFIPERWYLYPKMIKERSAFA

PFTTGPYTCVGKPLALMNIRATIARLITTFDMELPPGDDGRALERSMREHFSIYMAKDIQVHFQKRAI

#22 66% to 532C2 = CYP5078Ax

>CYP5078A5 BAE65602.1 [Aspergillus oryzae]

MIDAASVVILIIGLIVARLVTNRFRLSGIPGPSLAAYTRLWKLYNAWKGDHHHTEIALHRKYGSLVRIGP

RHISVSDPKAIPIIYGVNKGFTKTAFYPIQSISWDKKPQMNLFSTRDELFHRDRKRPIASAYSMTSILEM

EPAVDSYTELFLSQIRKMVEEKAPIDLGMWLQYYAFDVVGELSFAQKLGFLEKGEDVDNMIEAIRGMLTY

AMICGQIPEAHKVLLGNPLFPILLPQMETWDQVVVFTLKAINRRASLQRDGDLGKDEIDEAIGGKDMMSR

WLAIHNADPTRLSTRDLMVHLSANVFAGSDTTAIALRSILYHLICHPDKMAKVRAEIDTADQEGKLSNPI

SYQESNTHLPYFGAVMKEAMRLHPSVGGNLERHVPPQGVTICGHYIPGGTNVGINPWVVHRDPIVFPQPD

SFIPERWLDSTPEQLKEMEKAFLNFGAGSRSCIGKAISLMEMRKILPQLLREFDIHLHQNKSWKTRNVWF

VQQEEFICDLTPRVRS

#66

>CYP5080B2 BAE62337.1 [Aspergillus oryzae]

MYILLLLPVLIVSYHVLHLIYITLTTKSLLSVPGPFIARLTKLWYFDRVRRGHFEEDNVRLHERYGPVVR

IAPDHYSISDRAAVKLVYGTGTKFTKSAWYEGWKHPSPERWTLFPDRDVRRHAETRKRFSGLYSMSSLVH

YEEFVDHCADIFSQRLTEYAQREGSLNLGHWFQCYAFDVIGEITFGKRFGFLDQGDDIEGTIAALQKTMV

YSTLIGIYPEWHPRLFELLSHFSWSGAGGRTYIMRYVQEKIRRHSEPAKRDPEQGTLQTQDFLEKMILAR

DKDPEKVTDYHLFMMGLSNVIAGSDTTAISLSSIMYHLLHYPAVLEKLRREVDDFTAQGRCSARVTFKES

QEMPYFQAVMKEALRMHSATGLPLWRVVPAGGAEISGYYFPEGTTVGINTWVAHYDEEIYPDAKKFRPER

WIEAEADPERLKIMNEMYMPFGLGSRTCLGKHISILEMSKMIPRLIRDFDFTTTSQKWSTENYWFVKPTD

FVVKVRRRTSSAKQA

#67 42% to 532F1 = CYP5080B1, 41% to 57A3, 42% to 57B2

42% to 5038A1, 43% to CYP5080C2, 43% to 5080C1

in the CYP53 clan part B

>CYP5080E1 BAE63486.1 [Aspergillus oryzae]

MNDERIVLTQDKGQSYTAQLLYLIVAGYIAHTVYYAYWGPTARIQGPWLARFTRLWELLKVSKGHFEQVN

IDLHKKYGPIVRISPNTFSISDPSAIKQIYLGRTTLMKSKFYEPFGDPLDPNLFSETDIKKHAQSRKAVA

HLYSMSFLVTYEGSADRCNVQLCAKLRDFARHRTAFDVPTWMQFYAHDVIGEITFGEPFGMMAKGQDEHG

IADSIDETIAYASRMAVIPELHRWVSWFGRVAHLKTPFHNIQKYILEQIDSRSGSDSVGIDFLKKLLTLR

EDDKITQLDIEKTVGNNIMAGADTTAISLSAVIYSLLKNPGTETKLREDIDTLAAAGKLSNPVTFEQARH

MPYLQACIKEALRVHPAVGRPLLRVVSPEGLTIAGQYFPGRFQGNSRYQCLVAHYNEDIFGRDATTFRPE

RWLESDKEKLSVMEQSLLAFGAGVRTCIGKNLSMLEMSKVIPELYRQFEFELSESEGAWMTWNNWFVKPA

FECYVRLREGV

#38

41% to CYP5087A1

>CYP5087B1 BAE63940.1 [Aspergillus oryzae]

MSPRGKMVQPIETPRQAFLAHPLTVILVGIILSLLVRRLLWNLKQRELERLHGCERLHNEGGCLRYDFLG

IAKAIKLGFHFRRRTSLPYTNALFKRYGETYASNVLGYRLIFTCSAENIKHLLSTAFADFDSSPLRKPLF

QPITPDGIFTLDGPGWKKSRDQLRSRLSNLRKIVDLNQCERHFQAFLQHVPPNGQAFDVQACAFALALDM

QTLFSLGESVDALSFCQSREKKQFLEDLLFVKEKIVQDGFRGPLRYLYPKRRFLHCCKRARRYVIAHVSR

QLTGCSSMSEKAEGAQPTNAEEE

VSLLADQALSILLANDSMSTTLSGLFFCLSQDERVVKKLTASILDTV

GLEPPTWGQLGTLHYVRWVLQEAMRLFPAVVFNARVANKNSTLPTGGGTNGNSPVLIRKGEIVVFSTWAR

HRLGKDFGENPDEFYPERWEHLSGDMIGFIPFNKGPRACPGQH YAMIVLTYIVARIFQTFSTVSNYDTRE

WTERISMTLENENRVLIGLS

#141

57% to CYP5089A1

>CYP5089A2 BAE60720.1 [Aspergillus oryzae]

MVSSTAFIVVQVALAALAAHVIYQCYFHPLARYPGPFLARFTNLWRLFTFFGGQHHLSEQHLHDKYGHVV

RVAPNWLSFSDLHDFDAIYGFNKSVEKDDFYLFGRPRDNRVPSVFALKTDADHRQRKRKVVGPALTTAKI

TRYESVVTKHVDLFFTRADAASASRQDGEMAAVNLAPLAHRFTMDVMLELIYGPDVVSHPYTDSATGADM

CSAMRKLVKMAWSFSLCPSYGWIMNSRLISGVLRTLTTSKQGGPTGMMALMTSSHTMIFRRPEQVSLPGQ

PGIVKSWLDIPLDDSSRMTQDEVFSEAVNLVFAGPGSVAAALTAMVYQLGTQEGQLWQEKLRKEADVEAP

PFSLELQAVVKETLRHCASFPTAFPRVIRRGAETIVSTLPAPLPIGTTVSANSYILGRSRKIWGHDADQW

LPQRWLGDESQRREMEAKLVAFSKGSRGCVGKELAWLVLAKAVMAIIRRWKFVSVGELRGKSYLEMQYDD

CWIEYEELA

31 seqs have no matches on first pass to An seqs and Af seqs also Mg, Fg, Nc and other fungal

>CYP5093A1 Leptosphaeria maculans

GenEMBL AY553235.1

sirodesmin biosynthetic gene cluster, SirB gene

note: a second P450 SirC exists in this gene cluster

Sir C = CYP5082B1

MSTLQDFQIAPAILHRVTVRDASAILFCTLLTVFLFISQGTVRG

VKRVGKSRLRTLVTGETPLRFDLEKYGYLGYQQFSKKANKPFLVKIYGLDHYVLPVKY

LDSLKTVDHHRLSFAQSLNDF ()

26242 LNVDASLGDLVTHSDMEIAVVTKHLNPRL ()

TTLTPVLVDEANFAFEKELGKLE ()

TWKTVNALFLSAFLTNRTSGRVLVGDLCRDDNYLHAMMKYTES

VFSSGVAFNGIPLGPFRKIVYYLGARQHRRDLDNAAALVLPEIKRRMAAQAEDPNCRK

ENDAIQWNLDLPLASPKEGLPLRHAHRVLHLSFAATGTVAILITHMIYNVLMYPEYLE

PLREEVMACTKAHGGWTEKAMNEMWKLDSFIRETLRVQPPSVFTAYRTVKNQPFTFPD

GFTLPVGSRITFPTLPVGLDPENYESASEFDGFRFFRKREEARLAKKAYNWGATKIDS

SFLPFGYGNQACPGRFFGVRKTKILFGKLINDYDFSWAEPRSARPENIVIEGQILVNP

TPEIKIKSRVAAGM

#68 no strong matches

35% to 546B1 Fg, 36% to 546A2 Mg

>CYP5093A2 BAE63236.1 [Aspergillus oryzae]

MDYIHSLPSNPALGLSCCIIFILLAIVLHDVYMWKCLPPGPPPIPLIGNKFQIPSKHPWIKFEEWSNVYG

PIYTIWLGRRPTVVISDPSIASELLEKRSTKYSTRPRFVTMGEIYWDMASILVQPYGKEWLIRRRLLHSA

LTPRALDNYTPLQEAESSRLCYQLLESAHEWEPLFDRLASSIVFAVSYGHRVDSAQSPVIKQRLDFMQYA

SSLNVPGAHLVESFPVLKHLPDWIAPWKAEIKRRGRLEAEANMTLVRVVQQDVESAKESPGAEPLFNSLT

KQLLETRDSDPTAFPLSERDFSYIPASLFGAGSDTTSSTLCSAMLAIVTNPRTMEVAQLELDSVVGRDRL

PTFEDIPNLPYLRAFSKEVLRWRPVAVLGGTPHACSEDDYYRGYYIPQGTVMLGNSWAINMNPKYYPNPD

QFNPLRFLDMDPHLLPYLPKEYTASAEQEKGSGHPSKLGHSSFGWGRRICPGADLATNTLLITLSRLLWC

FDIRPIPGQTYDTLDYTNGFNIRPRSLHLSLQVRSDQHRQVIERGYEVATKFLERLSPFDERMQGNS

>CYP5093A3 AAJN01000232.1 Aspergillus terreus NIH2624

75% to 5093A2 no introns, 36% to 546B1, 35% to 546A2

49905 MEHLRTLLSSTLVWGAGCGILLIVLGILVHDVILWKRLPPGPPPFPLIGNKLHVPSKHPW 49726

49725 IRFRDWSKIYGPIYTIWFGRRPTVVISDPTVAAELLEKRSTKYSTRPRFVTMGEIYWDMA 49546

49545 SILVQPYGKEWLVRRKLLHSALTPRALDNYKPLQQAESARLCYQLLQHADRWEALFDRLT 49366

49365 ASIVFAIAYGHRVDDFRSPVVRQRLEFMQYSSSLNVPGAYLVESFPILKDLPDWMAPWKA 49186

49185 EIKRRGRIEAEANMNLVRVVQRDIESANEAPGTAHIFNSLTKQLLEIRGQDPASFPLTDR 49006

49005 DFSYIPASLFGAGSDTTASTLCSAMLAIVTTPMVLEMAQAELDSVVGYDRLPTFEDMRNL 48826

48825 PYLRAFCKEVLRWRPVAVLGGTPHACSEDDFYNGYYIPKGTVILGNSWAINLNPEYYPNP 48646

48645 DHFNPLRFLDVEPHSLSYLSEQYLSSTKQEKGTSHPSKLGHSSFGWGRRICPGADLATNT 48466

48465 LLITLSRLLWCFDIRPVPGRTYDTLDYSSGFNIRPRNLHVKIKVRSDHHGGVVEREYGDA 48286

48285 SEFLETLPPFEDSLPEHSPIKM* 48217

>CYP5093A4 AAGI01000340.1 Phaeosphaeria nodorum SN15

60% to 5093A2, no introns, 39% to 546A2, 37% to 546B1

MPLESVFKL

211788 PAYIWASLIFAIPLVALLHDVWVWFRMPPGPTPIPFLGNKHQLPKSKPWIQFQEWSKTYG 211967

211968 PIFTIWIGRKPTIVISDPIIAVDLMEKRSTKYSSRPRMVAMGEILWDGASILVQPYGKEW 212147

212148 STRRKLLHQALTPKALRLYKPVQTAEASRLCSQLLETPANWEKLLERFTSSIVFSVAYGH 212327

212328 RIDSLNADVIYQRFKFMHVAASLNVPGKYQVESFPILKHVPDVLAPWKAKIKAQGREEAA 212507

212508 ANMALVDVVRNDMARAKTRGEDIPDSLCKLLLEMKETEHIPLSDRDFSYIPASLFG 212675

212676 AGSDTTASTLCTAFLALVTHPETLQAAHSELDAVVGTNRSPTFDDEKDLPYLRALVKEVL 212855

212856 RWRPVAVLGGTPHASTEDDYYEGYYIPAGTTVLGNSWAINLNEEYYPNPHLFDPMRFLDS 213035

213036 ALSEKLKAPTGLTGKAHPAKTGHSSFGWGRRICPGAGLAENSLIIALAKILWT 213194

213195 FDILPVKGVKYDTFAYTEGFNIRPKSFRCEIRIRSEVHRKVLEEDFKSAEGVLERFTPFG 213374

213375 E* 213380

#154

>CYP5093B1 BAE56598.1 AP007154.1 [Aspergillus oryzae]

45% to CYP5093A1

81343 MASESSIPLYDCLIIGGGIAGLSSALSLVRTLHTAVVFDEGIHRNDQAPHLATVPTWDSQDPKRFRDAAK

LNILSKYSTVEFANVKLEKFNKALGKPFLTKVFGHDYVVLPSKYFDDIKRASPQSLSFFQALSDGLNMEA

SVGHLYASTTEIDVVVKHLNPRLTQLTPLLCDEAEYAIEREVGALPDWKKFNVSNLIAAIVHRTTNRILV

GKELCRNEEYLAITTKFSRSLFISGIFWNFVRLGPLRKLVAWLTIGLHLRDRNAAAKVLLPHVLARRQEK

EAGVDVATKYPDALQWTIDTAPSFPGDDEPLHQVYHMLHLTFAASSASGVGVTQCLLNVLAYPEYLEPLR

DEISTVVARHGGWTDKALSQMALLDSFIRETMRLHPAGS (1)

FMDDHFRFHDGLTLPKGTNIIAPALAIHYDP

DNYEDAHRFDGFRFARYRQKQGENHRWLASTIDQKFLQFGYGNHACPGRFYAIRKIKLVLAKLIMDYDFK

WAQPRPVHDRPEDFAIEAQLVAAPDAEILIRSRNLSN 78253

>CYP5093B2 AAKD02000026.1 Aspergillus clavatus NRRL 1

82% to CYP5093B1

366479 MASEQVPLYDCLIIGGGIAGLSAALSLVRTLHSAVVFDEGLHRNDDSPHLATIPTWDSH 366303

366302 EPKTFREEAKLNILTKYSTVEFADVKLEK (0) 366216

365065 VNKALGKPFITKVFGHDYVVLPSKYLDDIKRASPKSLSFFQALSD 364931

364878 GLNMEASVGHLYASTTEIDVVVKHLNPRL (1)

364727 NKLTPLLFDEAEYAIRKEIGPATEWKSFNVSNLIGAIVHRTGNRILVGPELCRDEAYLAAT 364545

364544 TRFSRSLFLHGIFWNFVRLGPFRKLIARLTIGLHLRDRDAAARMLLPHIHSRRQEKASGV 364365

364364 DVAAKYPDALQWTLETPSSFPGDDDPLHQAYQMLHLTFAASSASGVGVTQCLLNVLAYPE 364185

364184 YLGPLREEIKTVVARHSGWTDKALSQMALLDSFIRETMRLHPAGSCK (2)

TVMDDQFRFHDGLTLPKGANIIAPALAIHHDPENYTDGSKFDGFRFARYR 363825

363824 QKQGDSHRWLASTIDPKFLQ (2)

363709 FGYGNHACPGRFYAIRKIKLVLGKLLMDYEFQWATPRSVNSRPEDLAIEAQLMVAPDTE 363533

363532 VLIRSCRA* 363506

#33 no strong matches

36% to 681A1 An, in CYP53 clan part C

>CYP5094A1 BAE64919.1 AP007171.1 [Aspergillus oryzae]

14 P450 genes and 2 pseudogenes on this contig

MASLTGAAAVLLAFILAYSTALTIYRLFFHPLARFPGPRLAAATKWYEFYFDIIKSPGGQFFKELSRMHD

VYGPIVRVNPDEIHVRDAAWFEVLYAPNPTKRNKYRPSAEMAGLTLGIHGTVDHDLHRRRRMAIAPMFNK

QSILSAEHLIKQHIDELTDVFESYLGTNNPINLQTTFLAYTTDVLYHYMFDTDAGYQRDSGAAQQWRHSM

DAVAQATPFLKQFPSLLSRVALIPLPMLIWVLKRIQPDVAGLLGTHQLMASIVSKYMASKPEEDQDELIA

TKAVKPRTLFHAIEASSLPPHEKAPTRLAQEGLTVLFAGGETGSRLLAHTVYHLLKNPEILEKVRKEILD

AAGDSNQLPDMKALEALPWLTASVRESLRLRAATTSRLPLVTEKPLAYADWVIPPNTPVSMSHGDILHNE

DIFPDPMKFMPSRWFNASPQQNRLFVPFGKGTRMCVGMNFAYCEIYMSLAVILARFDLELYDTRWERDVH

YTRDCFLGEPDPASPGIRVKVVADHKTFTRS

>CYP5094A2 AAKD02000032.1 Aspergillus clavatus NRRL 1

77% to #33, 35% to 681A1

260809 MTSSLPIPLQVLNLG

260854 LSFIFAYLTSIIIYRLYFHPLARFPGPKLAAATKWYEFYFDIVKRPGGQFFQELNRMHEIY (1) 261036

GPIVRVNPDEIHIRDPSWFDVLYAPNPTKRNKYWPSAEMAGLTLG (1) 261228

261296 THGTINHDRHRRRRMANAPMFNKRSILSTQGLIKQHIDELVEVFASKSGTDEPVDLQTTFL 261478

261479 AYTTDVIYHYMFDTDAGYQRDPKVARKWRHSMEAVAQATPFLKQFPNLLSNLLRIPLPIL 261658

261659 IWVLKRVQSDVAGLLETHQ (0)

LMAKIVSKYMATKATEAQSETDEE 261838

261839 SKKVKPRTLFHAIERSSLPPDEKQPPRLAQEGLTVLFAGGETGSRFLAHTVYHLLCNPDI 262018

262019 LDKVKKEILDAAGESNEVPDVRTLEGLPWL (0) 262108

262165 TASVRESLRLRAATTSRLPLVVEQEMVYQNWVIPAN (0)

262345 TPISMSHPDILHNDSIFPEPLRFRPARWFTATEQQNRLFVPFGKGTRMCVGMD (2) 262503

262562 FAYSEMYLSLAAILPRFDMELYETDWERDVQYSRDCFLGEPDPRSPGMRVKVVAD 262726

262727 NKAFALTETAMVS* 262738

#160 44% to CYP5095B1

41% to 682D2, 36% to 551A1, 34% to 551A2, 38% to 682D1

note 682D1 probably has a small intron after the KYG

>CYP5095A1 BAE66500.1 [Aspergillus oryzae]

MGFTQDLVSVIRPSIESPLAFCATTVVAVALYVLVLGVYRITLHPLAKFPGPKLAAFTQWYETYYEFFKS

PGGQYLFHYRKLHEKYGPIIRLSPFEIHIQDSSFFEEMYSQSLPWDKPKELEHRFGNANGLFPTHKHEVH

RHRRAALNPYFSKRAINNAVPMMQEQITKLCDRLRREYQGTGKVFRLDWMMGCIASDIIVRYCVDRGYDF

FEAPDFKSPFIQALFDLLDGVHMITQFPWVATLFNSLPQGLVETLQPGMKSVHHFHKEMADQVAHILSNK

EKRNGSEQTNVFNALLDSDLPPEELTLTRLQQEAFTVIGAGFDTTRYALSVAGFHIINTPSIYQRLREEL

KTAIPDPSNMPSLTDLEQLPWLTACIQECVRMSYGVSQRAFRISDHITLTYKNYVIPPGTVVSMDNYSVA

HDEEIFPESFTFKPERWLGDPLAPDGKKLTRYLVSFGKGTRSCLGINLAYAEMYLTIANVFRNFDFELFE

TDRSSVDCYRDMFLPHVKLGSQGVRVKIN

>CYP5095B1 AACD01000118.1 Aspergillus nidulans FGSC A4 new not in known set

44% to #160, 39% to 682B1

73084 MTMIDLNPDLSFTAKSVALLLFIALGL

73115 LYTTIQVVYRLYLSPLSRFPGPRLAAATGWYEAYFELVHKGGGQFLFEIRRMHEVY (1) 73282

GPIVRINPFELHIADPAYYSTLYTSTLNLDKASHIQHRFNAPTAAF 73474

73475 STPEHALHRRRRAAISPFFAKRRIDEQAPMIQSHVNVICHRLSEEYAGTGKVLCVNELYG 73654

73655 SYVTGVIMTYAFNRCPRFLEEEDFVSAFGKSIQGLKEFVHWAQQFPCLPRILQNVPPVLM 73834

73835 GALFPAMQASLVFQE (0) 73879

73939 EMRMQAHAVLSGHGQKGTIFHDLLASDLPRSELTLPRLKDEAMSIVGAGIETTK 74100

74101 MASVVTTFHILQNPRILQKLQDELDSAIPDPTAPPSLSVLENLPFLAACIKEGIRLGYGA 74280

74281 TGRSPRISRTESLRYGAWSIPVGVMVSMDSYHMHHDESVFPDSYAFVPERWLDGARGPDG 74460

74461 TGSLNKYLASFSRGTRMCVGINLAYAEITIVLARLFRGFVMELYQTTRKDVDCYRDLVGM 74640

74641 EVVRGSRGVRVKVVGRRE* 74697

#78

39% to 682D2 but only 33% to 551A2, 32% TO 5095A1, 36% to 5095B1

>CYP5096A1 BAE61439.1 [Aspergillus oryzae] in the CYP53 clan part C

MMPTFFQAVCSTCIALAIYTASLAIYRLFLSPLAKFPGPTLAALTGWYEVFFDVLLGGQFMWEIERLHGK

YGPIVRINPHELHIKDPDYYNTLYAGPTRRRDKYLWFLSVGAPTSTFATPESDHHRLRRGMLSPFLSKQA

VRDLEGVIKAKLDLLCEHMKKAMRSGEAVELHAYFVSFAVDVVSTCAFGESGCFEELRRERLDDRWKNVV

TGAFGKLLLTRHFPWLVVVFRFLPVWATALLTPVVRHIDYMEKGVENQMQHVYAMNKDGVKENGIFSQLM

HNLKLPLKERVLYRLADDAKFLMVAGIDAPSQVLAITMFHVLRSPLVCRELREELQDKTAHSRDELCLNT

LEKLPYLTAVIKEGLRVSAVVTSRLPRIAPEETLEFRGWKIPPGTPVSMSSHFILRDPAIFPDPLVFQPE

RWLKQSTTGSSLDRYLVPFSKGSQGCLGPNMAHAWLYLALATLLQRFEFSLFETTEENIRTVRDCFNGQT

KPGQNNIRVKVLTEFI

#80

>CYP5097A1 BAE60821.1 [Aspergillus oryzae]

MDLTLHLILFLCFWACYLLFEKQLQQRSPLPPGPTPLPIIGNILQFKRKQAVWETISQWSDQYGPIMSFK

VASRTIIVLSTNQAIRDVLEKTPVNSSRPRFIGVNENLTRSMMPIFLPISDKWKAIHRVQLSLVNSRSAK

GIAEIQLLTAKQHLFNMLENANYDIADHINRFTSNVVSTVLFGTNIGSVSQAKSNGKFGLADQFIASICV

EHALVDLFPILEHIPGVARLGASKGNAWFESIRQQYTEDIQRAINSPTWSMIKAAHQQKIGGMSEDAFRM

WNVEMEFALGMTSSMMIANLVAMAVSHSHEFRQVQSEIDRVVGPERLPTADDLAHLPRLHAFVKEGIRVA

PIVPFSVPHAAVEENEYMGYRIPVDAIILPNQWHINREPKYFEDPAEFKPQRWIDNPNLPGPALVGYGIR

ICPGRQTANNGLLIVLALMAWGFDLNHAGGVKEPPNVLETLIMKLPTSSIRYSCRSSVHRDLIQKEWLAS

DDDPSSILNTLGSSLGF

#86

>CYP5098A1 BAE60012.1 [Aspergillus oryzae]

MGLILAFAVILLSTCWFLWYSRKAVFDRLPPGPRPLPFLKSRQELRQTRQWEALDDLHRQYGPLVGMTWG

GRPAVLIGKREIAKDLFGKRGSIYSSRARLVMGLDIMTGGDHVFFLPYGPKWKKLSRIQATFLNRPAVKH

YRPLQELESLHTLQDLLHSDDYEACFSRFQASLTHALAYGTRLHSATDPQLTELENIARTFISAATNSHW

MVDSFPILKYVPACLAPWKRFGQQIHAQTVSLFQGKMAVAEHTRSWNWVKHIRALKHTSGVTDHEMVYVI

GSIYQAGVGIITATLRLFIMACVLHPEAVKAAQDELDRVVGSDRLPTLNDLGHLPYVEAFVKEVLRWRPL

VLAATHSVTQDDDYRGYRIPRHAVILSNQWAMDMDREVWDSPEQFRPDRWMSDRKRMPSAFGLGQRMCAG

QYMAMESLLIMASRMLWAFTFEHAWEGGKRVEIDSWAFHEESLFLVPKPYRARIQPRDPHRLHVIQSTWQ

AAEKDIDPLLDQIGREIHTASA

#100

>CYP5099A1 BAE56799.1 [Aspergillus oryzae]

MEHHTWALFVDKLDYKAVFGLLLTIIIAVVTTQILHVYTKCCPGIPSIPFHISVYDAYRRVSEIGFHNSR

LRPVLETHGAVNIWNSGQWAVLVTKPEYVVRILRNERVVAKGGFYGKVPHSTLAGLFGENIIDSHGELWK

QFTGVMKPGIQRPHSISSLKVASSKLIATFKREQQHAPSDRGVVIDDIIERWAIDVFGESFFDVDFGALN

GGTVRAQDALLAILWNLGGHLIHHFPMFERIGWPLRPTRPHCFSMIRELEEALIDITEKLKYPETPPDRF

EKVIYRLKRARDDGLMTDFHYRSNLKMMFFAGHENVKFAFIATLWELSQNPQMQEKLYREIAAHIASSSD

GDDLKSLPYLTAVLAETLRLYPPVSQLINRKTLEPVYLGNGITIPQGMWVGWTAYGVHTDPNTWGPTAHE

YQPERWGDDVHAIQRAISQQQVRGSYIPFNAWTRSCIGSEFALLQLRVTLYEIVRHFEITSAPDYRYSIK

VIWSCAFLLRHRIYGRCSRVYGSSEGGC

#113

>CYP5100A1 BAE65915.1 AP007174.1 [Aspergillus oryzae]

29% to 639A1 very poor match to other P450s

C-term on gene model is not a P450 seq = MFS transporter

AEIFTPAVRQESDLDRKSNVTGGFLHGAVLISLLQYLPLLYQAVQLETAILSAISLLPIVIIGVV

IAAASMLMVPLFGGYVWVLRVGLDHTHPGNRLAGPV 1255617

1252988 MPAISLSLIGIGVVAVGTVWALWALTRKLQVPNNYPNAPRSLPYSIPFLKSTIPFVLDGLNLFRQAS (2) 1253188

1253239 LYCQDRWPLRVDLLNGEVYIVQGAKNIASIFSTPGLTVTQAYGIALKHCFGMEQKAVDAYLADTSGSWHRPIP

GSQTPWHGRVSYHTHENLVRGLLGAGLDPTTERIERLLLASLEGAVSRTSEWTYGMDLTEFFETHLGSAI

LQALYGPLLVTKNSDFNRNLWRYDKQIMRLAKRLSSWLIPEAYRLRDELLGAIMRWHQQATLLSETIPSC

ERTSGGEADPYWGSAMMRERNKMLLSIEGQDAKSVASTDLGFIWA (2)

SVTNVVPSTMTLCTHMYRDHSLVEN

IRLAALNCIRPGATLRFDLNKLGKQPLLLSMYAETLRFGVQIHIPRCSPHQPLSVAGVTIPPDKMIFINT

SLAHTDESVWNTRNGEHPLDTFWAQRFLIDPKDEGSGPTRKECSQPLESTPRGEYKRMDGQGSSSGQFTL

EGLDGSWIPYG (1) 1254606

1254672 GGQHACPGRILAKRIILLASSMMATMFDIELLAPNSLQFGSPRFGFGVRKPSAQVPFRI

RRRKSVSQDPFIC* 1254893

>CYP5100A1 AAIH01000177.1 Aspergillus flavus NRRL3357

98% to CYP5100A1 Aspergillus oryzae

22528 MPAFSLSLIGIGVVAVGTVWALWALTRKLQVPNNYPNAPRSLPYSIPFLKSTIPFVLDGL 22707

22708 NLFRQAS (2) 22728

22785 LYCQDRWPLRVDLLNGEVYIVQGAKNIASIFSTPGLTVTQAYGIALKHCFGMEQKAVD 22958

22959 AYLADTSGSWHRPIPGSQTPWHGRVSYHTHENLVQGLLGAGLDPTTERIERLLLASLEGA 23138

23139 VSRTSEWTYGMDLTEFFETHLGSAILQALYGPLLVTKNSDFNRNLWRYDKQIMRLAKRLP 23318

23319 SWLIPEAYRLRDELLGAIMRWHQQATLLSETIPSCERTSGGEADPYWGSAMMRERNKMLL 23498

23499 SIEGQDAKSVASTDLGFIWA (2)

SVTNVVPSTMALCTHMYRDH 23678

23679 SLVENIRLAALNCIRPGATLRFDLNKLGKQPLLLSMYAETLRFGVQIHIPRCSPHQPLSV 23858

23859 AGVTIPPDKMIFINTSLAHTDKSVWNTRNGEYPLDTFWAQRFLIDPKDEGSGPTRKECSQ 24038

24039 PLESTPRGEYKRMDGQGSSSGQFTLEGLDGSWIPYG (1) 24146

24215 GGQHACPGRILAKRIILLASSMMATMFDIELLAPNSSLQFGSPRFGFGVRKPSAQVPFRI 24394

24395 RRRKPVNQDPFTC* 24436

#114

>CYP5101A1 BAE65910.1 AP007174.1 [Aspergillus oryzae]

first exon may be wrong

MGRSRSTDWR ()

NSSALFARAKKEFRHKLCALRIAGQDMVMVTTAAQIAAIDKDSPTFAFEPFVDLVYDEIA

TVSRESKPLLWRTPAEGYKSLFPSAKQMTAAHTGIHLLHKQLTQPDAMHRFMTNSLVHVNNTLQWDSFYN

TSVLAATADVKVVSLECLCRDVIIDAQLTSFFGPRILEIEPDIRSFLKAWDSISWKVSYRLPSFLAKHAT

QLRDHLIEVLVKYYAFPAEERPGSVSFVNDVYDDYKQAGIPDRDIAGIVFTILWGLNSNVNAISYWMIAH

LMNNPTVVNEIREEIAPMMRALDSSPTIDGTILADVSKDPLLNSCPIMNSTFNETLRFTATGSSFRETTR

DTTMDGRRIPKGTKNIIPQRTQMMHSAAFGPDPETFDCYRFYRNKSLLRKVEFRGFGGGTTLCSGRVVGR

HQVLAYLALLFWRYDLEVIGPDQEVLGVRGKGFPRLDEAKPSLGPGKPIDGDDQILRLTRRNV

>CYP5101A1 AAIH01000177.1 Aspergillus flavus NRRL3357

99% to CYP5101A1 Aspergillus oryzae

12856 MGRSRSTDWR ()

NSSALFARAKKEFRHKLCALRIAGQDMVMVTTAAQ 12677

12676 IAAIDKDSPTFAFEPFVDLVYDEIATVSRESKPLLWRTPAEGYKSLFPSAKQMTAAHTGI 12497

12496 HLLHKQLTQPDAMHEFMTNSLVHVNNTLQWDSFYNTSVLAATADVKVVSLECLCRDVIID 12317

12316 AQLTSFFGPRILEIEPNIRSFLKAWDSISWKVSYRLPSFLAKHATQLRDHLIEVLVKYYA 12137

12136 FPAEERPGSVSFVNDVYDDYKQAGIPDRDIAGIVFTILWG () 12017

11949 LNSNVNAISYWMIAHLMNNPTVVNEIREEIAPMMRALDSSPTIDGTILADVSKDPLLNSC 11770

11769 PIMNSTFNETLRFTATGSSFRETTRDTTLDGRRIPKGTKIIIPQRTQMMHSAAFGPDPET 11590

11589 FDCYRFYRNKSLLRKVEFRGFGGGTTLCSGRVVGRHQVLAYLALLFWRYDLEVIGPDQEV 11410

11409 LGVRGKGFPRLDEAKPSLGPGKPIDGDDQILRLTRRNV 11296

>CYP5101A1 AAKE02005071.1 Neosartorya fischeri NRRL 181

98% to CYP5101A1 Aspergillus oryzae

runs off the end

17 QQMTAAHTGIHLLHKQLTQPDAMHEFMTNSLVHVNNTLQWDSFYNTSVLAATADVKVVSL 196

197 ECLCRDVIIDAQLTSFFGPRILEIEPNIRSFLKAWDSISWKVSYRLPSFLAKHATQLRDH 376

377 LIEVLVKYYAFPAEERPGSVSFVNDVYDDYKQAGIPDRDIAGIVFTILWG 526

594 LNSNVNAISYWMIAHLMNNPTVVNEIREEIAPMMRALDSSPTIDGTILADVSKDPLLNSC 773

774 PIMNSTFNETLRFTATGSSFRETTRDTTLDGRRIPKGTKIIIPQRTQMMHSAAFGPDPET 953

954 FDCYRFYRNKSLLRKVEFRGFGGGTTLCSGRVVGRHQVLAYLALLFWRYDLEVIGPDQEV 1133

1134 LGVRGKGFPRLDEAKPSLGPGKPIDGDDQILRLTRRNV 1247

#115

>CYP5102A1 BAE65870.1 AP007174.1 [Aspergillus oryzae] heme signature very altered only C is conserved

1128173 MVVCVITVLILGFLASVRALCNYTRLSTVPGPLFTGLSDLWRTYARSTSDYGCSLAGLHMKYGKVVRLGP

RSISVSDPTAIFPVYNGRPSER (0) 1128448

(?) YEGIIDQ

AANNLITSLRQRPLVRLTTLLQGFATEFINRLVLEESATQVMPFSAQGASCHWYNRTGKWLKLPMIEYVL

LRSPAARLKRRRGISFVPRKVQGGINGSQSSRAMNDGGISYNISLPRSYSNADGAYACIAMAFVSTFSSL

LKHENVMTRLRSEIDTAFSKGLLSDPPRWQELGKLRYLDAVIKESMRQLPSLGYNREVVTPPEGAIVAGY

YIPPGTMMELHSEALRDDPGIYGEDVHTYRPARWLTADLRQRWAMNQNLQQLSTSINDCPKVRVTWLELKKIVVLILLKFNVGLFSVTNSAFD* 1129557

>CYP5102A1 AAIH01000069.1 Aspergillus flavus NRRL3357

99% to CYP5102A1 Aspergillus oryzae

27804 MVVCVITVLILGFLAFVRALCNYTRLSTVPGPLFTGLSDLWRTYARSTSDYGCSLAGLHM 27625

27624 KYGKVVRLGPRSISVSDPTAIFPVYNGRPSER (0) 27529

27475 YEGIIDQAANNLITSLRQR 27296

27295 PLVRLTTLLQGFATEFINRLVLEESATQVMPFSAQGASCHWYNRTGKWLKLPMIEYALLR 27116

27115 SPAARLKRRRGISFVPRKVQGGINGSQSSRAMNDGGISYNISFPRSYSNADGAYACIAMA 26936

26935 FVSTFSSLLKHENVMTRLRSEIDTAFSKGLLSDPPRWQELGKLRYLDAVIKESMRQLPSL 26756

26755 GYNREVVTPPEGAIVAGYYIPPGTMMELHSEALRDDPGIYGEDVHTYRPARWLTADPRQR 26576

26575 WAMNQNLQQLSTSINDCPKVRVTWLELKKIVVLILLKFNVGLFSVTNSAFD 26423

1: P S E R V T F Y D T S P S Y L C T D * F

2: L V K E * R F M T H R R R I Y V L I D F

3: * * K S N V L * H I A V V S M Y * L I S

CCTAGTGAAAGAGTAACGTTTTATGACACATCGCCGTCGTATCTATGTACTGATTGATTT

121 ---------!---------!---------!---------!---------!---------! 180

GGATCACTTTCTCATTGCAAAATACTGTGTAGCGGCAGCATAGATACATGACTAACTAAA

1: R S Q Y L N M A I G S Y S P K A R S R S

2: V A N I * T W R S D R I H P R L V H E V

3: * P I F E H G D R I V F T Q G S F T K S

CGTAGCCAATATTTGAACATGGCGATCGGATCGTATTCACCCAAGGCTCGTTCACGAAGT

181 ---------!---------!---------!---------!---------!---------! 240

GCATCGGTTATAAACTTGTACCGCTAGCCTAGCATAAGTGGGTTCCGAGCAAGTGCTTCA

1: R P I L K V V * * A R W I D G G L W N Q

2: D R Y * K * S N E Q G G S T E V S G I S

3: T D I E S S L M S K V D R R R S L E S A

CGACCGATATTGAAAGTAGTCTAATGAGCAAGGTGGATCGACGGAGGTCTCTGGAATCAG

241 ---------!---------!---------!---------!---------!---------! 300

GCTGGCTATAACTTTCATCAGATTACTCGTTCCACCTAGCTGCCTCCAGAGACCTTAGTC

1: R C D M K A S L I R Q P T I * S L L * D

2: D A I * R H H * S G S Q Q F D H F F E

3: M R Y E G I I D Q A A N N L I T S L R

CGATGCGATATGAAGGCATCATTGATCAGGCAGCCAACAATTTGATCACTTCTTTGAGAC

>CYP5120B1 AACD01000093.1 Aspergillus nidulans FGSC A4 new not in known set

this p450 belongs to a group of sequences without Cys at heme region

weakly similar to CYP532

38% to AAJN01000022.1

MVAWA

214086 VLLLAVVAATLLHLLYNYSRLKSVSGPILAAFTDAWRANAQRSSSSSEYGRLLKELHRKY 213907

213906 GVAVRLGPGFVSLSEVGDITRVYHTLLQDE (0)

213714 YESSAADTAMRNLVHTVRRCRTVDMTTLLHFFADEVNTRLFWSACA 213577

213576 PSAASSASSRTHRIQPSFSFFATIEELVLRGPVALLKRERLSCYCLSGDVSAKIYGNE 213403

213402 LVLPTGPSATRKLAISAPDGLILAASIQIITKAFVSVFFFLLNNPLIMRRLR 213247

213246 QEIESIPRFRNRTKLPSSRDLGGLYYLDAVFKETMRLVILQSQPMEVRVTFESLYISSKH 213067

213066 VPRGTVLSWHPHVVLTNDAIYENNLYVFRPERWLTPNRQRQTLMEASLLPFMVCRIHYPK 212887

212886 LEAAWLLLKKTVVVLLREF 212830

CDVSLSWLLCSSLSISLRSRHDSFGGLLTSSFL*

1: Y H T L L Q D E V C L S G P P A L E W W

2: I I L C S R T R S V Y L D P Q P L N G G

3: S Y S A P G R G L F I W T P S P * M V D

TATCATACTCTGCTCCAGGACGAGGTCTGTTTATCTGGACCCCCAGCCCTTGAATGGTGG

1: I A N W I * V R R L G Q E A P L P E H Q

2: * L T G F R * G D L V R K P P Y L S I R

3: S * L D L G E A T W S G S P P T * A S D

ATAGCTAACTGGATTTAGGTGAGGCGACTTGGTCAGGAAGCCCCCCTACCTGAGCATCAG

1: T V Q Y E S A A D T A M R N L V H T V R

2: R C S M R A P L I L P C A T L S T Q Y

3: G A V * E R R * Y C H A Q P C P H S T

ACGGTGCAGTATGAGAGCGCCGCTGATACTGCCATGCGCAACCTTGTCCACACAGTACGC

Note if A. nidulans seq was modified by adding red bases the

Protein seq would be

ATTAGTGCACCCCGACGGCCTCATCCTCGCTGCGAGCATTCAGATTGTATAACGAAGGCTTTT

I S A P R R P H P R C E H S D C I T K A F

Red region would differ and would resemble the related sequences

>CYP5121B1 AAKD02000042.1 Aspergillus clavatus NRRL 1

39% to AAKE02000008.1 Neosartorya fischeri

MAAFG

82938 ILLSAVLVLGGVLLRNYVSLNTVRGPLLAGISNLWREFLQSAPEYGRRLESLHRKY 83105

83106 GQVVRVGPNTVSVSDPSLVAWIDLSDRGTVN (0) 83162

QLTSTFLRAVRKQRTINLAVFKLFAADIVQHM 83430

83431 CSNDIIQPRASQVPFATDPFIPTESSYVSSFLNSLLLKCPIAMLKHKRNGCLPI 83592

83593 CGFVPESVKRPNAYQPVAGEYQRSGNVSLVAIGVECMEATFASVFYFLSTH 83745

83746 PATLSRIKREIDTIFSMASFSEVLHQTKSYALPYVEAVMKETLRHVMIFDYQHQVPPG 83919

83920 GVMVGEYDLPQGTVIQYHADTIRHNSTTYGEDSALFRPERWLLADTTERKRMEEGLIPLR 84099

84100 ICFRNDSQTRAAWRSLKMIIALVISKFDV 84186

1: N M V R W F V S G R I R S A S L I L L S

2: I W S G G S C R A E Y G Q R L * S F S R

3: Y G Q V V R V G P N T V S V S D P S L V

AATATGGTCAGGTGGTTCGTGTCGGGCCGAATACGGTCAGCGTCTCTGATCCTTCTCTCG

1: S L G L I F R I E G R * M Y V I A L P D

2: R L D * S F G S R D G E C M L * P F P I

3: A W I D L S D R G T V N V C Y S P S R Y

TCGCTTGGATTGATCTTTCGGATCGAGGGACGGTGAATGTATGTTATAGCCCTTCCCGAT

1: T Y C F A D N A E T S F G N D I G T A T

2: H T A L L T M L R R P S A M T * A Q Q Q

3: I L L C * Q C * D V L R Q * H R H S N R

ACATACTGCTTTGCTGACAATGCTGAGACGTCCTTCGGCAATGACATAGGCACAGCAACA

1: E S S V D P Q Q Y A S P H E Q R I E Q L

2: S H L S I L N S M H R R M N N A L S S *

3: V I C R S S T V C I A A * T T H * A V D

GAGTCATCTGTCGATCCTCAACAGTATGCATCGCCGCATGAACAACGCATTGAGCAGTTG

1: T S T F L R A V R K Q R T I N L A V F K

2: Q A H F * E L F A N N E P L T W Q F L S

3: K H I S E S C S Q T T N H * L G S F * V

ACAAGCACATTTCTGAGAGCTGTTCGCAAACAACGAACCATTAACTTGGCAGTTTTTAAG

>CYP5121A1 AAKE02000008.1 Neosartorya fischeri NRRL 181

42% to AAKD02000042.1 Aspergillus clavatus

1210404 MAVFAALLLLLCVFGGILWNYTRLNTIPGPLLAGLSDIWSQCLKSSPRYAYRLQRLHRKY 1210583

1210584 GEVVRIGPKTVSVTDPSNIFWLDAACPQRSK (0) 1210676

1210852 DLVLDFLKAIRKQRTMKLTVFRDFATDIVGRMFVGDLEDDRAAPMRTGSNQMDRCRFTTS 1211031

1211032 LDHIMFKNPVAKLKRKRNKSLRWVSCAVTHSRWDDASIFEVLPETGRKTFLQ 1211187

1211188 AETREYGGPYASLIVKGADCMVAAFVSVFRHLLKCPTAMSQLQHEVDNAFRNLTLSD 1211358

1211359 ILQQETELHALSFLDAVIKESMRLALRFDYLRDVPAGGLAVLGHYLPEGTVVQFHSEA 1211532

1211533 LRNNRTIFGEDVSDFRPQRWLQADLDQWQRKQMEALLFLRPNMPNSAKARAAWLELKRA 1211709

1211710 AALIILKFDV 1211739

AGCCCTTCCCGATACA

AGAAGTCGTTTCTTGAT

TACTGCTTTGCTGACAATGCTGAGACGTCCTTCGGCAATGACATAGGCACAGCAACAGAG

AGCTATGTGGATGAACCAGAAGGAGCGACTGTCAATTCTCAGCTCAAAAGCAATCATCGT

TCATCTGTCGATCCTCAACAGTATGCATCGCCGCATGAACAACGCATTGAGCAGTTGACAAGCACA

GTTTACCTAGACGAACAGACAGACGATGGACCTCAACATAATGATAAAAAGGACCTGGTTCTCGAC

1: C S V F I A N M A R W F A * G R R R S A

2: A A S S S Q I W R G G S H R A E D G Q R

3: Q R L H R K Y G E V V R I G P K T V S V

TGCAGCGTCTTCATCGCAAATATGGCGAGGTGGTTCGCATAGGGCCGAAGACGGTCAGCG

1: S L T P P T F S G W M P P V H K G R R Y

2: H * P L Q H F L A G C R L S T K V E G I

3: T D P S N I F W L D A A C P Q R S K V F

TCACTGACCCCTCCAACATTTTCTGGCTGGATGCCGCCTGTCCACAAAGGTCGAAGGTAT

1: S S L P F Q A D G L Q A E I * K S F L D

2: H H F L F K Q M D Y K L R F R S R F L I

3: I T S F S S R W I T S * D L E V V S * *

TCATCACTTCCTTTTCAAGCAGATGGATTACAAGCTGAGATTTAGAAGTCGTTTCTTGAT

1: S Y V D E P E G A T V N S Q L K S N H R

2: A M W M N Q K E R L S I L S S K A I I V

3: L C G * T R R S D C Q F S A Q K Q S S C

AGCTATGTGGATGAACCAGAAGGAGCGACTGTCAATTCTCAGCTCAAAAGCAATCATCGT

1: V Y L D E Q T D D G P Q H N D K K D L V

2: F T * T N R Q T M D L N I M I K R T W F

3: L P R R T D R R W T S T * * * K G P G S

GTTTACCTAGACGAACAGACAGACGATGGACCTCAACATAATGATAAAAAGGACCTGGTT

1: L D F L K A I R K Q R T M K L T V F R D

2: S T F S K P S E N N V P * N * L C S E I

3: R L S Q S H P K T T Y H E T D C V P R F

CTCGACTTTCTCAAAGCCATCCGAAAACAACGTACCATGAAACTGACTGTGTTCCGAGAT

>CYP5120A1 AAJN01000022.1 Aspergillus terreus NIH2624

36% to CYP5102A1

33059 MVTLAIVVLFVLIASIRLLYNHVRLNTIPGPVLAGLSSFWLHAQPSSQLPNSLLQLHE 33232

33233 KYGTIVRIGPDLVSVSNPATIVLIYTRRSRNR (0) 33328

33462 YEGLVDPALRNLVHTLRRYQTLDLASFLHFFAADFLSRLTTLHPAGANNA 33611

33612 RRHEPTERGLPGFFAECLLLRGPISRLKRQHGCPFICREVRSHFTGQPTSISSRTLFK 33785

33786 SEDHPSPMEEHLDCIAKTFVSTFRFLLKYPAVMRKLRREIVAAHNHNNLSPLPLW 33950

33951 SELGELKYLQAVMKESLRLSHVHRVHEVKSPAGGVTVSGYYVPQGTTIRCHPLVVHNNA 34127

34128 QIYGDRPQVFDPERWLNNDLQRQKCMTECLMLFQQHVFENPTIYAAWWELKKAIVVVLMW 34307

34308 FNVSGIQQ* 34334

1: D L Y A A I A E * G E S S C K C V E E *

2: I Y T R R S R N R V S L H V N V S R N D

3: S I R G D R G I G * V F M * M C R G M I

GATCTATACGCGGCGATCGCGGAATAGGGTGAGTCTTCATGTAAATGTGTCGAGGAATGA

1 ---------!---------!---------!---------!---------!---------! 60

CTAGATATGCGCCGCTAGCGCCTTATCCCACTCAGAAGTACATTTACACAGCTCCTTACT

1: Y Q S L S S * S T I Y N A Q R L V M S I

2: T N L C L A K V Q Y T M P S D L S C P S

3: P I S V * L K Y N I Q C P A T C H V H P

TACCAATCTCTGTCTAGCTAAAGTACAATATACAATGCCCAGCGACTTGTCATGTCCATC

61 ---------!---------!---------!---------!---------!---------! 120

ATGGTTAGAGACAGATCGATTTCATGTTATATGTTACGGGTCGCTGAACAGTACAGGTAG

1: R P K P S A A D S P T H S S M K G S L I

2: D R N R L Q Q T V Q R T A V * R A R * S

3: T E T V C S R Q S N A Q Q Y E G L V D P

CGACCGAAACCGTCTGCAGCAGACAGTCCAACGCACAGCAGTATGAAGGGCTCGTTGATC

121 ---------!---------!---------!---------!---------!---------! 180

GCTGGCTTTGGCAGACGTCGTCTGTCAGGTTGCGTGTCGTCATACTTCCCGAGCAACTAG

1: Q P C A I W S I P S D D T R P S T W P A

2: S P A Q S G P Y P P T I P D P R P G Q

3: A L R N L V H T L R R Y Q T L D L A S

CAGCCCTGCGCAATCTGGTCCATACCCTCCGACGATACCAGACCCTCGACCTGGCCAGCT

>CYP5121A2 Aspergillus fumigatus Af293

GenEMBL AAHF01000006.1

80% to CYP5102A1

2336635 MAVFAALLLILCVFGGILWNYTRLNTLPGPLLAVLSDIWSQCFKLSPGYAYRLERLH 2336805

2336806 RKYGEVVRIGPKTVSVSD 2336859

PSIVFWLDAACPQRSK (0)

NLVL

2337098 NFLRAIRRQHTMKLTVFRNFATDIVGRMFVGDLGDDRAAPIGTRSNHIDRCRFTTSLD 2337271

2337272 HIMFKSPVAKLKRKRNKGLRWISCAGTYSRWNDASIVDVLPETGRKTILQAETRE 2337436

2337437 YDGPYDSLIVNGADCMAAAFVSMFRHLFKCPTAMFQLQDEVDNAFRNLTISDVLHQET 2337610

2337611 ELHALPFLDAVMKESMRLAMKFDYRRAVPAGGLAVLGHYVPERTVVQFHSEALRNNRT 2337784

2337785 TFGEDVSDFRPQRWLQADLDQWQRTRMEEALLFLRPNIPNSAEARAAWLELKRAAALIIW 2337964

2337965 KFD VSTVGSPAEVKLIYLSCILSTMRKCSSKMLSLQSKN 2338081

1: D A A C P Q R S K V F I T S S P S R W I

2: M P P A L K G R R Y S S L P L R A D G L

3: C R L P S K V E G I H H F L S E Q M D Y

GATGCCGCCTGCCCTCAAAGGTCGAAGGTATTCATCACTTCCTCTCCGAGCAGATGGATT

1: T C * D S E V V S * * L C G * T R R S D

2: H A E T Q K S F L D S Y V D E P E E A I

3: M L R L R S R F L I A M W M N Q K K R L

ACATGCTGAGACTCAGAAGTCGTTTCTTGATAGCTATGTGGATGAACCAGAAGAAGCGAT

1: C Q F S T Q K R S S Y L P R R T D R R W

2: V N S R L K S D H R I Y L D E Q T D D G

3: S I L D S K A I I V F T * T N R Q T M D

TGTCAATTCTCGACTCAAAAGCGATCATCGTATTTACCTAGACGAACAGACAGACGATGG

1: T S T S * * N Q E L G S Q L S Q S H P K

2: P Q H H D E I K N L V L N F L R A I R R

3: L N I M M K S R T W F S T F S E P S E D

ACCTCAACATCATGATGAAATCAAGAACTTGGTTCTCAACTTTCTCAGAGCCATCCGAAG

#116

39% to 54C1

>CYP5103A1 BAE65635.1 AP007171.1 [Aspergillus oryzae] removed some seq

14 P450 genes and 2 pseudogenes on this contig

46% to AAGI01000167.1 Phaeosphaeria nodorum

37% to 54A1, 38% to 54B1

MEVQYILGILHNLDLAIWAVLIFIAFVLVTQAHYKAQLAKLPTFETDKGNEKWRRTYSDSARMLYQEGHKK

VSYLFKDQAWIMPT

SDGRKNVVVPGRLLSE

LSKLPETVLSFPTAINKVLEVKYTKVAPDEPFAPYCIK

ADLNPALSRLNPIIYREVENALEDEIPQCEDWTPVFIYRKLLNTVAKVSGRIFVGAELSHNKDYLDTAIN

YTIELGNAVQAVKQMKPWLRPFLAWKLPEVQQLNKREEMAIRFLEPIIQARREASKDPDTKSPMTCCSGS

LLRRIVKMQLLVIFAGIHNTTVTATNVLYNLAVSPEYMQPLREEICKAISDNDGTLTSRALQQVEKLDSF

MKETIRFYPQNSLPSRAKRGQYIPPGTSIEAPLQAIYQDDSNYPDSDTFAGFRFYKIRQGGGATVHARNQ

FVTSNEQNLVFGYGKHACPGRFLAAAEIKMIVSKILLTYGFKNADNWTERYSNYEVGRLNMPERAIPLLF

RKTTAQDSSL

>CYP5103B1 AAGI01000167.1 Phaeosphaeria nodorum SN15

46% to CYP5103A1, 41% to 54A1 some gaps in seq

4489 MDVEKLAGLAYTFQTITIASLVFVVLTYAPKLMRRLQLAKLPSMPGKDYATS 4334

4333 ARKLYKEGHQKV (?) 4298

4170 SIGEHSVVVPPHFLPELRKLPDDVLSFAAAIDN (0) 4072

4025 VMETKFTGVDSEDSNTVHTIKSDLTPALPRLNQAVCTEVDAAMREALPSCEDWTEVDIN 3849

3848 DKLLDIISKVSGRVFVGPDLCHDPEYLELGRNYTVYLMEALFAIKRTRPWLKSFVGHRLP 3669

3668 EVKRLRDVEKKAIKFVQPIVRERLEAEKSDPNWQKPDDMMQWLINRYNKDGHVSVEHIAK 3489

3488 CQLGLILAASHTTTMTTTNILYTLVSTPEYVEPLREEIRNAMHGNGGSITTRALQEMVKL 3309

3308 DSYMKEVLRLYPAG (?)

3165 GQYLPPGATVQVPTAAIYTDSENYPDPDTFDGFRHSKLREGGTATDHSRNQFVTTNETNL 2986

2985 GFGYGRHACPGRFFAANEIKMIVARLILNYDMKMPGDATERYPNVDIGRSCIPCPGKTLM 2806

2805 FKRV* 2791

#120

>CYP5104A1P BAE64914.1 AP007171.1 [Aspergillus oryzae]

pseudogene just upstream of CYP5117A1 about 800bp, missing C-term

14 P450 genes and 2 pseudogenes on this contig

MQQNMIIPSFWTGTAIIGLVACAYVSYQCLLSPLARFPGPFAAKLSKGWRAYKTANGQWHRKLVDLHRKY

GHVVRIAPNELSVGDPSSFRKIYSPAEAGNGFNKAACYSVVQGNRPFDLTGERNEKVHSEQRKLVATAYS

MSSMVHFESKVNVVIETVIHKLEARCRKTIDLGHWLQMWAFEPTRPLLNSLKPDVIGSVSFSQPFGYVES

GDDEGVFKRIQNAMGSAAWLMHAGWLFRLHQKLIPICGNWLAVNDRNGYFFQVACREVSGRINRGGDDKD

IIGQLLETQKIKPQLKDLDISFMMTSNVFAGSDSTSIAFQSIFYLLLTHPAAHDRLMRELREREEKGELS

DPVSFQEAESWPYLQAIIYEAMRLYAPAAFVLDRVVPPEGMMIEDKFVPGNTVVGSSAWVIHRNPEIWGP

DVDTFRPERWLDD

ERYFFAFGGGSRTCIGKSMCSSTAYICFLTNVCFRYQLA*

>CYP5104A2P AAJN01000022.1 Aspergillus terreus NIH2624

probable pseudogene 54% to CYP5104A1P

MKLLALLLDSSAIATLLLA

68053 VTTGYVVYQVFFSPLAAFPGPFWAKVTYWYWAWRSMRGQAHRDLVNLHQRYGSVVRLGPD 68232

68233 YL (2) 68238

68297 ERGKSKSFQEIYS (?)

PEEAGGKFRKSAVHDTVTGTRPFDLLAQRDE 68476

68477 KIHSGQRRLVASAYTMDSIVYLEPMVDSLIVSTMEKLSTLPGDIDLGGWIQLFAF () 68641

68712 DVIGAISFSRPSGFIEAGDDGGIFRRMQNSLSSISWLMYVDWFYSLYQRLMPVIGNWLAA 68891

68892 NDRNGHFFNFARQEITARKDRAGNTKDMASQLFAVQHTKTELDDTNIAFMMASNVLAGSD 69071

69072 TTSTSARAVIYLLLKNPKSLQRLLDETEERKSDGRLSYPVKFQEAESWPYLQAVMYEAMR 69251

69252 LYPAVGRNLDRDVPAGGLRIGDHWVPEG (0)

TMVGSTAWAIHRIP 69431

69432 EIWGPDVEDFRPERWLDE (?) 69485

ERYFFAFGGGSRTCIGR (?)

VLYYLRLIVLQFVPTLFMRFDVDLVPGTALEEFCG*

#123

36% to 682B1 missing PKG motif, frameshift at YIL/PGT, this restores the PKG motif, possible pseudogene

>CYP5105A1 BAE64645.1 AP007171.1 [Aspergillus oryzae]

262325 MTLFQVVETSFILLALWTSVEAIRRLFSHSLSHIPGPRLAALTWWYEFYYDAVQSGQYVFKIQELHKQY (1) 262531

262599 GPIIRITPDEVHINDVGYLDTIYAPSMTRLDKYDYQLRTLRVPGGVGTTADYYLHRIRREALPPFFSKRNV

LWLESVITEKVNQLCGLIAKHAATETPVNLSDAFYGFSNY (2) 262931

VVNNFLFAHQTDVLADEQEAARLRHNSHEL

LKGINMNKHFPWIPDILEALPQLLTRPTMPPGLIDMLELFD (0)

RVRAELITIITRISSNTSGEKESINTGAK

GSVYESVLDSPNLPASEKALLRLEQEGALLTLAGTESPAQTLNIIFYHLLANPSLLTKLREELATLPTLS

TWTQLEQLPYLSAIIEEGNRLSFGVTARTARIQHTPITYTPSAYVTTPDPTHKSYIL 263721

263721 PGTPVSITTLSAHTAESVFPDPYAFLPERWLGD

EGRERRKFQLTFSRGGRKCLGIELARAELYLVTAALVRKFDLVLWETDERDVSFEHDYHVAMPRDGSRGV

RVVARIRGGLALSRDRLTTK 264089

>CYP5105A1P AAIH01000184.1 Aspergillus flavus NRRL3357

pseudogene with 4 stops, 96% to #123

38647 MTLFQVVETSFILLAL*TSVEAIRRLFSHSLSHIPGHRLAAFTWWYEFYYDAVQSGQYVF 38826

38827 KIQELHKQY (1) 38853

38921 GPIIRVTPDEVHINDVGYLDTIYAPSMTRLDKYDYQLRTLRVPGGVGTTADYYLHRIRRE 39100

39101 ALPPFFSKRNVLWLESVITEKVNQLCGLIAKHAATKTPVNL*DAFYGFSND (2) 39250

39309 VVNNF*FAHQTDVLADEQEAARLRHNSHELLKGINMNKHFPWIPDILEALPQLLTRPTMP 39488

39489 PGLIDMLELFD (0)

RVRAELTTIITRISSNTSGEKESINTGAKGS 39668

39669 VYESVLDSPNLPASEKALLRLEQEGALLTLAGTESPAQTLNIIFYHLLANPALLAKLREE 39848

39849 LATLPTLSTWTQLEQLPYLSAIIEEGNRLSFGVTARTARIQHTPITYTPSAYVTTPDPTH 40028

40029 KSYILPPGTPVSITTLSAHTAELVFLDPYAFLPERWLGDEGRERRKFQLAFSRGGRKCL 40205

40206 GVELARAELYLVTAALVRKFDLVLWETDERDVSFEHDYHVAMPRDGSTGVRVVARIR*GL 40385

40386 ALSRDRLTTK 40415

>CYP5105A2P AAID01002562.1 Botryotinia fuckeliana B05.10

pseudogene 61% to CYP5105A1, 36% to 682B1

frameshift at KIQ, also after PIMP, one stop, runs off the end

1431 MAFAQLLGTGIILSLILTIAEATRRLYFHPLAHIPGPKLAALTWWYESYFDVIQPGQYVF 1252

1251 KIQ

NLHKQY (1)

GPILRNTPDELHIQDVGFLDTVYAPSASPRNKY 1072

1071 EYQLRTLRIPGGVRTTARYDLHKKRRAALSPFFSKRNVLHLEPLINKKVEQLYQMIDKHA 892

891 KEQSPAN*SDLLFAFSND (2)

VVTNFLFNHQVDVLSDETRAVELRH 712

711 NSHQLLMGININKHLPSIPDFSESLSLSISKPIMP

PGLIDMHAPFD (0)

519 RVRTELTDIMSSKPSEVSSEKLLGPTKKESVFASILDSAILPPTEKTLLRLQQEG 355

354 SLSVLAGTESPAQTLKIIFYHLLTNPAIIAKLRAELDTAPTPTSWATLEHLPYLSAVIDE 175

174 GNHLSFGVTARTARIADEQLTYTPSSHVTSTTNKGTFYKIPTGTPISITTLSAHTAET 1

#124 revised

>CYP5106A1 BAE64452.1, BAE64451.1 AP007170.1 [Aspergillus oryzae]

137250 MSFASALILVVAIWCIYVGWPWYRNWKQAKALHVPIVISPISTS

GAALQSLRYILDRDILPTWITRLAFVRLIQRNSRFQEKFAVHAEYGKLFILVTPATCE

LYVADVDAAKQVLSRWRDFPKPSSLLGMIFPERNA (1) 137663

GADWQRHRKLTARAFNEKLHEAVWAESTRNATKV

137909 MTKWNNTKPVYSTRSDMMALSLAVLFKACLNIDGDDKDDTRILAGDVAACQWHLDVVLKGISNPMALGRG

FEGIKKLKRSHKALGELLTEFVEARTIRPKLSAHADLLSSILAPTDHRGLSSDEVTGNLFLFMFAGHETT

ANALIYIIHLMAIFPAWQDWALEEIDQLSHNRADGEEVPSYSQILPQTQRLRAIL (0)

YETLRLYGPVPTLVRQTDPQAQTLVLPEQEVIIPKDTPVNVNTIALHTDPKQWGPDPLAWR

PDRWILQPCDSSLQKVSNELLKYLFAWGDGPRLCPGQRFSQIEVFAVLICFFKS

HRVELVPSRDQTMDQARSHALSLIQNSKVGLTLQMPDAESVGLRWVGR*

>CYP5106A1 AAIH01000668.1 Aspergillus flavus NRRL3357

only one amino acid difference

#128

42% to BAE63454.1 [Aspergillus oryzae]

>CYP5107A1 BAE63455.1 AP007167.1 [Aspergillus oryzae]

601589 MIGEQYTSIITGFKSALAVSCIAVSLFLLSPWIAYARLPSSIKSPIKAKGPLSALRACLNEISAGAKTST

RGYELY (0)

SKKGQSFAMLNINFRPQVILPPEHVRWLVTQPEDILSHAKASDDADALGYIWPLFDASALHSFS

KVLQIDLTRNVTQTEKDVLEEVQHIMDELVGQTESWKEVNMVQAFERIMYQATQRVYVGLPLCRDSTYMG

YVKGYARSLGTAMVFAAQLTPWPLRQVTALLAGLPVYYYVLRVRSYLSPLFKERMERLKEKGGTQDDNLE

GEPRNLITWMSNGVLSGVGPKSISPSEMVTWLGIL (?)

602662 ALLPTDNLWTTCTNVLLDLLSSESEHAYLHTIREE

ARTVFASSKESGKPVSHGLHHIDSAIRESLRMNSLSPRSLHRQVVRRGGVVLPDGQKVPTGTWLCVLSGN

IQRDEDYYEDAQTYKPFRFVPKLTEAGGDKAPLLPLTNEKYLTFGYGRHAC (2) PGRWFSFQVMKIVIAYILA

NYDIQPLEKRPDNIVFADLNIPHLSHIIRIKRMT 603339

>CYP5107A1 AAIH01000006.1 Aspergillus flavus NRRL3357

only one amino acid difference

complement (71337-73087)

#129 only 679bp from CYP5107A1 pointing away from each other

42% to BAE63455.1 AP007167.1 [Aspergillus oryzae]

one stop codon, possible pseudogene or seq error.

>CYP5071B1 BAE63454.1 [Aspergillus oryzae] heme signature incomplete

600910 MQIADMAASTTAQILVVSLGLLIFVLLCPWFGYLRLPSSMRWWPSIPSGPLSALRLSLKEYSGSRSSENG

YKAFSKKAEPFAICNPSFYPQVLLPPEQIPWLLSQPENVLSHEKANEDVHALPFLAPAFDNYDHLELIRA

IRTDLTRNIPNTEDAFLDELRHTTNEVLGAPGDNAWKEVNLTVALDSIIFGICLRLFFGVSLSRNRTFVY

YVKIFTRVTGAMMLFVSQLVPWPLKPVVGIVAGFPIYYYWVRLIIYLYPTFKERIQCLRTKKETPPADMV

TWMVDLAISQNPTRKVHISSLIVRLTLININVRLTRSSKVFLPVDVLIAMTDNFFLDLLSSDPDRKYYNA

LRQEAEAAFTNRDKTQPISQSMPYMESTIRESLRLSPLSDRMLSRRVVHKGGITLPDGQFLPRGTWLAVA

AVGVHRDERTYEDPDEYRPFRFLSEDTETKEAKAMLVPVTSEKFLAFGHGRHSC (2) 599384

599313 PGR*FAAHAMKLIIGYILVNYDIEP

LEKRPVNSVVGQTIIPQLDVKIRVRRRE 599155

#130

>CYP5108A1 BAE63248.1 AP007166.1 [Aspergillus oryzae]

all the best hits are CYP51

but at low 20% range, revised at N-term

1834519 MLEALLFRPSLLSFISAWLGTLGLALLLFSSRRASTKLPPRANGGIYFI

SDILVFLASPVQFVKQATGKHGSVFRIDCLVKQIFYLRGQKWNRFFLEMK

EDTWSFSGGI (0)

1834137

GMFLNKAANPGYFTHGRNLLGSINRGVNRSAALQSYGRLAGEEAHKSLQHWSQMPDVEIFESISRFVHRVI

VRCMMGEDFYDHHVDELYDLLQQMESLVGHPFNLLLPSWVPHLPGRQLAQARNRFAEIFRERLAARQLES

DVWRDSLDYINYTLNDPRTAQLADYYPSHHVVLMFAAHTSTVASIAWTIVE (0)

1833514 LLRHPIYQEEIRESLATMSDIHQCAPLLACLREEG

RRYSGVHMFRTTKQPVSLEGSDYTVPEKSVVLISPYLTHHDPAIYPEPHEYQP

HRWLLPDGRLNPWNGSKEAAFLQFGAGNHRCPGENFAAIIAREFLAALLMKYEIEWGRDGAPADLSRLDF

TKVGSPWLEGDAAVRIRPRVFGS 1832966

>CYP5108A1 AAIH01000996.1 Aspergillus flavus NRRL3357

99% (4 aa diffs) to CYP5108A1 Aspergillus oryzae

6424 MLEALLFRPSLLSFIAAWLGTLGLALLLFSSRRASTKLPPRANGGIYFISDILVFLASPV 6603

6604 QFVKQATGKHGSVFRIDCLVKQIFYLRGQKWNRFFLEMKEDTWSFSGGI 6750

6806 GMFLNKAANPGYFTHGRNLLGSINRGVNRSAALQSYGRLAGEEAHKSLQHWSQMPDVEIF 6985

6986 ESISRFVHRVIVRCMMGEDFYDHHVDELYDLLQQMESLVGHPFNLLLPSWVPHLPGRQLA 7165

7166 QARNRFAEIFRERLAARQLESDVWRDSLDYINYTLNDPRTAQLADYYPSHHVVLMFAAHT 7345

7346 STVASIAWTIVE (0)

LLRHPIYQEEIRESLATTSDIHQCAPLLACLREEG

7540 RRYSGVHMFRTTKRPVSLEGSDYTVPEKSVVLISPYLTHHDPAIYPEPHEYQPHRWLLPD 7719

7720 GRLNPWNGSKEAAFLQFGAGNHRCPGENFAAIIAREFLAALLMKYAIEWGRDGAPADLSR 7899

7900 LDFTKVGSPWLEGDAAVRIRPRVFGS 7977

>CYP5108B1 AAJN01000021.1 Aspergillus terreus NIH2624

43% to CYP5108A1

MLTQELQAIYANNAGFLITSL

70767 LAFALLALAVRSFLRSEKIPLVSAGLPILGNLQKYSLDPVGFIASATRLHGECFAVPML 70943

70944 FGRTIWLRSPQLNKEYLETRE (0)

DVWSFGDGMGLFLNKIVIPGYF 71123

71124 SHLRSMVGSLSRGISRKATLEYYAQVADEETAKAVDGWTCKSEAEKSVALFEEISFLVHK 71303

71304 IIVRCLMGQDFYDHHVRELYDLLRTMEANVGSIWHTVLPGWVAHGPARRLWRCRQRVQEI 71483

71484 FDFRLRERERSPEEWKKRLDYISYTLQDPATAHLSRFYGAHHTLLMFAAHTSTVASISWI 71663

71664 LLE (0) 71672

LLRSPHRLQRLREELATHALEQSPFLDALVKETG

71834 RHYSGNSDVRWARKPKTLRTEVASVPESRITIPEGTIVSISPYLTHHDPATWDNADTYLP 72013

72014 ERWLADPDLAKKMNEGGQLRYIPFGAGSHRCPGEKMAILIAKIAVARIVQSCDLAWGEGS 72193

72194 SENTLGGLDFSKVGSPWLKGDVQVRFQ 72274

#133

36% to 573A2

>CYP5109A1 BAE63087.1 AP007166.1 [Aspergillus oryzae]

1411903 MSPFIFAVTLTFAILALGILRRRYFHPLSRFPGPFLGSVTSLYQTYWHVHPNKTLHDTELHKKYGPIVRY

SPNGLIVNDPALLPVIYNRRANKTDFYAPVFDTHSTFTRKDYREHVASRKAISHAYSVTNTRLFEPQVDG

ILSELISLLSESATEKRLVDIMDWFTYDVTSLFVCGKPFGFVEKRTDVKGLIQNKNKVLFIIFIMTIQEN

LSWIVRNTRLGRRYLMPHPTDQSGLGVVMAERDRIVDAVIDNDGKVKRHLLVKGSLLSSLMEILGTEGCP

LSLVDVKAEIFFAMLAGSSVTPSQLARVIFHISRNFKVQEKLYEELVAAEQDGRIPPLSAIISDEQAHRL

PFLSACIREAQRYAPTMSQLPRYAPEGTGLELHEQYVPPGTSVSTSPWIIGRNKDLYGEDANSFRPERWL

EASPEEERRWDHFSFHFGYGARKCLANNFGLMQLYKVAAEVFRRFEVKVEGSNEDTVSGGPPASARFRFD

RRARSWS 1413728

>CYP5109A1 AAIH01001109.1 Aspergillus flavus NRRL3357

4670-6495, only 3 aa diffs

#143

>CYP5110A1 BAE58993.1 AP007157.1 [Aspergillus oryzae] gene model revised

1141014 MAIQSTLEMCHLAESGVYLILVTFGVGLLFGLIVLTSDYLDGWLRRRALGDIPFVDEGSN 1140835

1140834 MSACLRWWSRKFDCDKEYAEAYKQ (0) 1140763

1140699 YSKTGKPYATRLKNNDHGIVLPLNSTKEWRTLPHDQLSFLHALSE (0) 1140565

1140509 FADLYMHTNITDRTPLHAVHYCNNTKTL (1)

SRFNRLMVDATDRALPLIVGKDTES (1)

EWKRANAFHTILSLCSTVAMSVLLGPEFSMDTSLIQTIM

MYNTAIMPSCAK

RTSYPRILRPFVWRLSPLCRAVKSDLLKTKIKLIPEIKHRIDIARSKKWWLEEGPMSLLDGLIETAFEKG

CLSRSSDRGDDDQQVALLAEEIIFYHFELSMPVVFFIIFAVYVIMNNKEYLTPLREEISEALKLSGGSFT

LDTLNHAPKLASFVKETCRLYDISCCN (2)

FRRVMKPIHLESINLSLKPGTIIMAPGRDVHLDPDYYDNPTTFNGYRFYD

ASRGTCTPHISTTSPTFLTFSHGISACPARVLATQITRTIFIMFLLKFDVELAHEEMPAYGFANGPAYLP

NPSVMIRVRPCQKDVLGV 1139078

>CYP5110A2 AAKD02000022.1 Aspergillus clavatus NRRL 1

55% to CYP5110A1

MAVQASSSIHHLADLGTSAALTGVVLVLLCGLLIVISDYLDGWQQRRALKGIPIVDEG

SYMRPLLRWKRFDAAKEYARAYHL (0)

YSKNGQPYATRLQNDDYGIVMPLNSAKEWRALPHEELSFLHALAE (0)

FVDMNLYSDVTDRTPIQAVHSCNNTQSL (1)

NHLNKLLVRNIDNVLPSIFKQPTQD (1)

165619 KWLEVNTFQTIMSLCSTVTMSLLLGPDTPYDPVLHHHVSSFGAAIMESCYGRTAYPRILR 165798

165799 PFAWRFSPQCRGLRTHLSMARARLIPEVARRVAAARKANIEVKDSRPTSLLDALIEAALE 165978

165979 NGSLNRDGICTNEKAQLQILADDLLFFHFELSKPTTVNIVFQMYAIMNHREYMSPLREEI 166158

166159 TKALELTNGEWTVETLKYAPKLESFTKETFRLYDISPCN (2) 166275

166335 FRRVMKPVTLKSIDLSLRPGTILVSPCRDIHQDPDFYENPMTFDGYRFYDTNSNACTPRV 166514

166515 ATTSLTFLSFSHGTGSCPARVLASQICRTIFIKFLVEYDVELANKKMPAYGFTNGPVFLP 166694

166695 NPEVMMRIRPRGNGMQ* 166745

#147

>CYP5111A1 BAE58483.1 AP007155.1 [Aspergillus oryzae]

3848877 MLQDLIATLASGAQMYWASSGVWMRVVLTVSAIWIFWRIWRFTILPLFRPNEPVELPYWIPCKAPYAKMV

EELSLTSSRLCLLTCMMIRTCGKRFGTLEPFSMIIAGDRYYVITSPEDTRPFFADVKAMTTDGFLDRALL

AFGCAPERLHTLWQRNTPTKVNPKGKNLIHLTQDLFKHDLVPGPTLNVLLERYQGALDELLSWDRLVGAY

PSLVSTQTEAISLYDICADFIANANQIVLFDRALLAIDPDMAVEMRTFTDELWKLVHRSRLVDTTEVTRI

LRQYSSAFKSYLQLPPEARPNETPVIRTLLETYAELGIHEDDRAAMLVMICWAGDANAYKAAYWVLAYIL

YDPQLREIIRQETAPAVGPDGKLDWPYLAKRCPRLSSIYHEVLRLTKRDVIVRQVVRDTALAGKRLRKDS

IAVIPTCQLHDNPDTYGADAASFNPDRFLKQPGLAQTTFFPYGGGRHYCPGRYFVEMEIYGLVALMLNRY

EMDLAWAAPFPRRDESLVTLGISRPVPGDDLHVTLNWKEGNKN 3850672

#150

35% to 551A1, 34% to 551B1, 32% to 551A2

>CYP5112A1 BAE57727.1 AP007155.1 [Aspergillus oryzae]

1498000 MNTSLDSSTALGFYITLTVVTISVAYVSILALCRLFLSPIAVFPGPPLARLTYLTQWYYDWVKDGQYYLQ

IEEMHKKYGPIVRVTPGELHIRDSNFHSKLYVTGAVRKSDSYHRFTQGTGFEDITFPLLSHDRHRALRGQ

VSRLFTRAGVRQHESRIVARIHVLSQRMCGFMSNGKPVNLHDAFSSLAAGMYNAISVIFHEEPTDYLGDP

EFNTAWYNTLKMGTATIPLLTQLPWLARVISAPVARYILERATPWRIFDDKSRRQMLKSKSRPTIASSDR

SNATVFDNLFKDNLFYTMNEQTFTRSAQ (0) 1496760

LIQQGAVHNVSLSLAMTIYCLLCQPDEQEKLTRELESSFSKYPERSPSFSELESLPHLSACIKEGLR ()

LSVGALVGIPRVSPDVDLVFDTYTIPKG ()

TPVSMTPHWMHMDPDVFPNPNSFDPSRWSSTLTEPEKLSLMGRYFVPFGKGSRSCIG ()

TRLAYLLLYQTLAHLFRPGAPRLLLHETDISDVTPVRGFLFSL

PKKDSKGLRVILVNDSESTNRM* 1495794

#155

>CYP-un2 BAE56589.1 [Aspergillus oryzae]

pseudogene C-term part only

DDSLWTDPTSFDGYRFEKLRTIKGNELKFQYASTSTSELNWGYGTHACPGRH

YASNQIKLMIVSLLSRYEFQFDHEQT

DKKAIVERPPNVVDGVRIMPNPQTLVMVRSLGNVNEGCE

>CYP-un2 AAIH01000268.1 Aspergillus flavus NRRL3357

note CYP65AB1 is on the (-) strand from 448-2267

pseudogene 98% to CYP-un2 Aspergillus flavus

RSSASQISDCPTDWKSVALFPTMVKLVSLHISRSFIQSPLSRNQEWV

IDLTLDYAISTVTVAGKMSNTHWALRPFKGHFLPETAEMSRQFTRARELLRPTLEARLQQ

RDEVPNDLMQWIINNYPDQEDDLTLHTRLQLEAVQAATY

YDLLAHPEYLQPLRDEILSVSESCNGHWTPAALSNLRKCDSFLKESQRLNP

PFRLPGGSTVPAGVSVSAPSMMVNL

3887 DDSLWTDPTSFDGYRFEKLRTIKGNEQKFQYASTS (2) 3988

4047 ASELNWGYGTHACPGRHYASNQIKLMIVSLLSRYEFQFDHEQTDKKAIVERPPNVVDGV 4223

4224 RIMPNPQTLVMVRSLGNVNEGCE* 4295

>CYP-un3 AAKD02000041.1 Aspergillus clavatus NRRL 1

pseudogene, 63% to Cyp-un2, 39% to 503A1

SVSVSRFALSPFRLPDGSTVPAGTSVSAPAMLANT

28289 DKMLWDDPLVFDGYRFEKLRAIGDNEQKFQLSSTS (2) 28393

28450 TSELNWGYGTHACPGRHFASNQIKVLMAALLLRYDFRFAKDQNSDTEYK 28596

28597 RPSNVVEGVRIMPNPNVEVLIRNRVEM* 28680

#135 only top part = P450 C-term, 36% to 584E1, 36% to 584A1 , 35% to 584B1

35% to 584C1, 40% to 584E5, 38% to 584G1, 32% to 584D1, 32% to 584D2

41% to 584E3

>CYP5113A1 New fam. BAE62292.1 AP007164.1 [Aspergillus oryzae] revised

MTAETGFVPTVFSLPSESAYSWVW

AVSAGLVAIFVLYLTQGIARRRFAYAHGCQAPPRYAHRDPILGLDSLRDSMQAR

KSDRYFRREQQLHQAYGNTFMSLLLGSWMVNTIEPKNLEVLFSTKFADYEVGFRRRNAFA

PLFGKSIFQSDGTRWQTLRSQLQLCFSRVQTSQLGLLESHCQRLLAALPSDNQKFDLALF

LHRFAADVSTDFLFGESINSLENPQNLDGGALKAFADTHSTCELRWLLGSMSWIWP

QRTFMKNVRLTHRFIQRYVDAALEREVTPPGKASDQQNEQRILFIDQLRQRTQDPIALR

DELTTLYFAGTDAPAALLINLFFVFSKRPDVWDRVRSEVQSLGGKAPDLQQLKGLRYVQD

CIREC (1)

LRLYPPQ

1582777 PSNSRVAVRDTVLPTGGGPDGGSPVLVPKGMMVHLSVYALHHRKDLWGEDADEFRPERWSYEKQTW (0) 1582974

1583043 KYIPFLGGPRNCVGSN (2) 1583093

(?) FGLNEVAYAVVRMAQNFQTITSVDPDEWVEGSSIALESKNGVKVVMCRDA*

#127

33% to 548D2, 33% to 548E2, 35% to 548F1, 37% to 548G1,

38% to 548A6, 41% to CYP548H1

>CYP5114A1 BAE63475.1 AP007167.1 [Aspergillus oryzae]

657999 MELPIRYCLGISLAVAGVAFCLYQMFFHPLAKYPGPTLAKFTAARAAYCAWKDDVHLDIWLCRKKYGPII

RYVPNHLCFDTVTGLKDIYLSGKDLRKDKIMGKASFVPNLLTIMDRKEHARHRRLIGIGFSQSAMKSVNE

RLLHHVKSFYHVAYNAGLPDEANGWSHPLTMSDLCSYLTFNVMADFIYGKSYDLIHCPDYRHLLEHIEES

NLRTGVLLYCPQLYIGRLDRKLFPRASTGNKTIHSFINQIIQERKSENGVGQSIYEQLGTQRKSTDHPLT

PEEIRSEAMLLTIAGNDTTSTALCAALFYLGKNLHAYEKLAAEIRTKFSVVDGIGQDETLRNCHYLHACT

YESLRMSPPVGSSMWREVGPGGTSIDGEFIPCGYGVGTGIYSIHHNPKYFPRPHDFIPERWLSEKDGFIC

KEQADIPFAAYILFSAGTRACLGRHLAITELLTTIAALVLLYDFRISHTENGELGCGHALGRHGRTNPGE

FQLYHRVTSGKEGPILQLRPRKGN 656192

>CYP5115A1 AAGI01000308.1 Phaeosphaeria nodorum SN15

39% to 5114A1

37% to #127, 39% to 548A3, 37% to 548G1, 38% to 548A6, 34% to 548D2

MNYLSVLVVAVAVTLLY (0)

43655 LIARVIYLAFFHPLAKYPGPFLSKFTCARASYYAWRGDIHIDIWRCHEKYGDYMRYGPNQL 43473

43472 YVNTPKGLRDVYGPTTSNKFLKSSHYEVMTHQAANTFTHRGGKEHLRRRRIMAQAVSTKA 43293

43292 QLEYEPRLVGHIQKFCNAVFKGEPMNMAKWCNYLAFDSMTDVVFGAQYNL 43143

43142 LGDEKFRYVPEMIEKSNVRISSLVQFPGLTWLRLDKHLFQEAIYARNRFLKFVFRLLSDR 42963

42962 RALSHGTARGVFENPSISLDVYSRLQDARDPVTGEGFGHDEIASESTTLIVAGSDTSACA 42783

42782 TASILFYLANNPHAYARVAEEVRAVRSSRPGHLAAADLAGCKYLRACIDEALRMSPPVGT 42603

42602 GLMREVAAPGGLVVDGHVVPMGCEVGVGTYAIHHSTRAFEDPFVYRPERWLAPDESAHAR 42423

42422 ACFVPFSAGIRSCLGKGLAYTEVTLTIGHLLWLGDFKLVADAELAAVGRGSRK 42264

42263 LGVDGRHREGEYQLYDHIAGQKNGPWLQFTRRAEVQ* 42153

#159

>CYP5116A1 BAE54596.1 AP007150.1 [Aspergillus oryzae]

This P450 is unlike any other, very low seq similarity

240943

MSLFLVIVVVLLLFLSSPLHRLVHIGLKKPVSPNIDPETYRRIIEGKGLYQEVHNQLNARQSRAIPNKRL

RAVFGIENALTTDNEIHAKRFLRQVKGLINLSPTSWESLSGLVRSAARRSIDGAMANSPDKPRVMLTNLV

QVLVLRVVLSVLFRMETEALEVPDHDLLRLAEAINDAWTSSKDKTHLVSFQDNISLQNSLKTVFPHLNCL

DPQGNPLNLVIPGFETMWRIVLRLFIEISYTSGLYHPEWRGIMTTFAIAPTKDEFERRNGKQNLSAEMLV

NEALRLYPPTKRVYRAFLPVGSDTVEVLNADIEKAQTATHIWGSDAEVFAPGRWGALTPQQKLAFF

PFGSKPFVCPAQAAFGPRAIALVVGALLVELGGEWSLCVGAECTEALVPGVRLSNQRTEYRDLCLARAGPV 242193

>CYP5116B1 AACD01000061.1 Aspergillus nidulans FGSC A4 new

37% to #159

363827 MTARSMASTVSLYLILFVLFVLFYRRCYRSSKKPAEFRTTAECRAIIDGSQFPDARKNQLTRL 363639

363638 EARALPNQPLIGAFGIKNAFTTGNEAEAKDFVENVRRLIEVSAVDWHGLAGALQCMLESI 363459

363458 IEENRDGARVKVSLTPTVQALVLRESFWILFQMGEDAHLEFKQLADLGKITNS 363300

363299 TWVRMKEERALEFKDNIVLQACLTAVFANHKTDINILDPGSNPLKLILPSFETVWRIVL 363123

363122 RLFIVLHCHDNEDYKRALLEFVRDPTLTQFRLRPDNAVSVEFLVKEALRLYPPTRR 362955

362954 IRRAFQFPGSSPNNQISNIGRANVEACHLNEEVWGPDALEFMPARWSKMSSVQRRSFLAF 362775

362774 GGPPFLCPASHAFGPMVIGLLAGVMLDVFGKMNGYGKEWVLGSDDERDMSEVHSRERLRN 362595

362594 ERDAYGGLFLDLYSRRIDVSS*

>CYP5116C1 AJ883817.1 AJ883817 Trichophyton rubrum CHUV862.00 cDNA

45% to AACD01000061, 45% TO #159

STSTRHSSHHKCGTS

913 VVLTXFYAXLQTDVDKTQYKDVLKLAEDINRIWISSKSPDEEKRSSYQSETPFHDTLKAI 734

733 FPNTQISSPASNPLNLILPSFETMWRIVLRTFLEINFLTGNRSPEFKQVLVEFFHNPTKE 554

553 QFNQELSPGVSASNVISEALRLFPPTRRVHRHFQGLSSNERTLFAADIEACHRNLRVWG 377

376 SDALKFVPGRWNCLNEQQKSSFMPFGSKPFLCPAKPVFGPRMIALLVGALLCHYTENWEL 197

SSVSGKYGINMVAEGEYLDRDACAQLYLVRDIRV*

#34 40% to 65E1

>CYP5117A1 BAE64915.1 AP007171.1 [Aspergillus oryzae]

14 P450 genes and 2 pseudogenes on this contig

MNSISALFSAGGFQWILLSLSLAFIVVYSLFYLAVGLYNLYFHPLARYPGPLLGRASSLWYARSLARGTV

AQDTLKLHEKYGDVVRIAPDELSFIQPENWSAIYGHQLGKDYRELIKDPRYHDTVKPTPTILTGDWDEHT

FYRKILSNSFSEKSLKDQEHILHHFVDLFVQRLKETSAEGTRELNMTDQWNYLTFDVIGFLTYGEEFHCL

TSSKLHDWIEAMLCVAILMSLGQAARHLPFPFDKIYKQWAIPSNVKRQVALHRDLTEVAIPHIPIQYASA

YRLNSRKGDIPYSVLKEHANILTIGGSETTATLLAGATFHLGKNPPVLQKLATEIRTTFVNDGEITVARL

SECKYLLATVEECLRIYPPSPANHTRMVPKEGIVLNDQHIPGGIGVGMPMYAAFRASSNFTYPDRFAPER

WLGDPMYSKDKKGALQPFSFGPRNCLGRHLAYQEIKLALAKLVYHFDLELNPKCGDWDEQKNFTFWVKPP

LWVNLHPVKS

#70 39% to 65S2

>CYP5118A1 BAE63025.1 [Aspergillus oryzae]

MFVLPTTFMSVSHGIYQLLFLALLATAWRIYSNRFRHPLSHYPGPFFWTISRLPYAVTYAQGTLHRRVRQ

LHDVYGDVVRVAPDELSYRTEQAWKDIHGYSRNFPKDMRFYQTSKNKAPSVVIAPDGVHGRQKRSILRAF

SERAMKSHEHLLRPFVDSLIQKLQHASTSTEGGAVDLTEWYNYIMFDFMAHELFGQSLGCLENGVNHPWV

DMLFGSIKVWAFLSQSKYFPNLSWIIKTAVRLFCRDLLNHRSKKLGCIASKIPEGEGSDSSLPTFNSFLR

ANKGPHSTLSTEEVLSNHSFMMMAGSETTATLLSGCTFFLLKHPQVYKKLTSEIRNRFSSSTEMTFSSLA

NMAYLRAVLQEALRMYPPLPLGMPRVVPPGGAIISGQFVPEKTSVAVASWATYQSSSNFNTPQMFLPERW

LDTGPGDNDVKGAMQPFSVGPRACPGKNLAFGEASLILARLIWEFDLELSPQCSSWAYQRAYIIWDKGPL

LIKLTPRI

#88 35% to 5068A1, 34% to 5068A2

39% to 5068B1 [Aspergillus oryzae]

>CYP5119A1 BAE60008.1 [Aspergillus oryzae]

MLPTLPNIAGRINTMATFLLPVAIGTIILLFLYGKYVTSTLIPGPPTLPLIGNLHQLPSDDRRHVLAQWH

KKHGPIISLKFGWSSVVILGNIAVTKELFGKRSLKYGSRPRMVMARDCMTKQMQTSTLPWGEKWKIHNRI

QLSLVGGPKIRSYQSLLDIESCKVLYQLLSTESLVTCFNRFKFNIIYTLAYGKDPDQNESDFHEILELAD

HFTQTLTNATWVVDLFPILNCLPRRLAPWKAVGDDFHRRAMGWFRRNSEAAVKSNSWNWTKHVQFNEDTG

NLSVSEMQYLIGVLFEAGVDSTATVLHFFVLACTLYPDAVTKARQELDKVVGSARLPTPKDLPQLPYVKA

FIQEVLRWRPITAEGLPHFTLEDDKYQGYDIPKGSTVIFNYWSGHMDEDTYQHADQFCPERWIERPDLPL

GVFGYGRRACAGRRLALMSLETLIPKLLWAFDFRSPAGTDHGKSRDPGTEHQGALIKPRSFPVSWHPVSN

DRRLIIERLFQERDKDLDTVLDDIGKAFERY

$$$$$$$$$$

#161

>BAE71333.1 putative monooxygenase [Aspergillus oryzae] NOT A P450

MDPANRPLRVVTIGTGISGILMAYQIQKQCPNVEHVLYEKNADVGGTWLENRYPMAGCDVPSHAYTYPFA

PNPDWPRYFSYASDIWNYLDRVCKFFDLRRYMVFHTEVVGCYWNEDRGEWTVRLRQHASGSEPREFEDHC

HVLVHASGVFNNPQWPQIPGLHDRFQGRVLHTARWPDDYQESQWKSDRVAVIGSGASSIQTVPGMQPTVK

HLDVFVRTGVWFGVLAGNTGSQTKEYSPTERDEFRRNPAALVAHAKAIEDQVNGMWGAFYTGSKGQAMGS

AFFRQRTANLIKDERLREGLDPPFAFGCRRITPGDPYMESIQKENVHVHFTPVVSCTEKGVVGGDGVERQ

VDTVVCATGFDASYRPRFPIVGRDGVDLREKWKECPNSYLGLAVPEMPNFFTFIGPTWPIQNGSVIGPLQ

AVSKYVVQWIKKAQNENLRSFVPRQDRTDQFNDHVQEWVKHTVWKDNCRSCTFLLSPRSQLNLLRTI

#7 Not a P450

>BAE71334.1 oxidreductase B/ cytochrome P450 monooxygenase [Aspergillus oryzae]

MRRYAILGATGNTGQALLNVLLQSPDNQIHAYCRSASKLNRLHPEITQHRQVKVWEGSLEDVSLLSECIR

GTRAVFMVVAIPDNMPYCTIAQDCTNAVLNALKRLQAEGCQSLPKLIVLSSASLEDSLCADVPPLIHRIL

NIAAGNLYSDLAEAEKTLRAEKHWVSTTFVKPGGLVHDVQRGHTLSTTTAKTPVSFLDVAAGMVEIANVD

DKRYDMMNVSVNAIGDGTAFPWKGVYYMMTGLLFHFFPWTYKYFGDCPMARPKKDI

#8 Not a P450

>BAE71327.1 cytochrome P450 monooxygenase [Aspergillus oryzae]

MVTYALLGATGATGSSILRHLLHDPPDSLRIQILVRSKIKLLQAFPDLQTRRNPQVHVIQGTSTDADALS

ECLRNATIAFMCVAQNGSPIGTTLCQDSARAIISALQQQQQSEGASYQPCTIVQLRSASLNPALAVQVPV

FVHPIVSFCLFANYADIKQACQYYSEARKQGTLEYIFVDPPTLHDANGTQSTGYRLISTESQATALSYAD

LGAAMCEIAQRRSEFHGRAVGVTATGSVHQTWGVLLRHLLEGGSARLREKIAKNTVVVGIVCSFLVALAY

LM

#108 63% TO SEQ 109 oxygenase not a P450

>BAE66426.1 [Aspergillus oryzae]

MKGVPSSAEASNSIPGSSLPSQRAHEPLTHKQPTLDHPRKQSVQSISSEHGGMAENFSKITKVVQAARRP

LPTETGDGTYIEPENGGSLWRDLRALGIKDANTLKDLIENKAGGLVKGSGQVVDDKTMLMERIIQLVAKL

PTESRNRVKLTNMFLGELWDSLPHPPLSYVGDKYAYRSADGSYNNPTLPWLGAANTEYARTIEPLKVRPA

SLPDPGLVFDSLFARDTFKPHPNNVSSVFFTWASLIIHDIFQTGHPDENFNKTSSYLDLSILYGDNQEEQ

NMMRTFKDGKIKPDSFSEPRLHALPAACGVILVMLNRFHNHVVEQLAAINENGRFTKPPERILDPVEARA

AWAKYDNDLFQTGRLITCGLYINITLYDYLRTIVNLNRDNTTWTLDPRAHMEHDTVPTALGNQCSVEFNL

AYRWHSTISRKDEAWTEQAYQAIVGKPGSEATVEDLMSGMRRLGANMPKDPSKREFAGLKRQSSGKFKDE

ELVDILTMAIDEVAGSFGARNVPKVLRSVEILGMEQARRWNVGSLNEFRKFFDLKPYQSFEEINSDPEVA

DQLRHLYEHPDNVELYPGIVAEDAKKPMIPGVGIAPGYTVSRAVLSDAVALVRGDRFYTKEYNSRNLTNW

GYEEANYDLEINQGCVFYKLALRAFPQYFKQNSIFAHYPMTTPSANRDIMKMLGREEDFSWDRPSYTPPR

TTLFDYANVRRILEDSSNFRVIWDEATGYVFGKGGYDFMLSGDSPFHANQRRIMKESLYRSQWHEAVKEF

YLEITEQLLSEKSCRVGNVNQIDISRDVGNLAHVHFASNIFSLPLKTKEHPHGVLTEHEMFDVMSIIFTA

IFFDVDPSKSFRLRHMARKAAETLGPLVEANVKAVSSASFLSTLIDGIRSNKNALSGYGVHMIRRLLDHG

LDASEVTWSQILPTAVAMVPNQAQVFTQIIDYYLSDEGKEHLPNIQQLAKEDTPASDEMLLRYVMEAIRL

NGIFGSYRKSHTNLTLDDKNKMVQIKPGDTVFVSFVDANRDPNVFPNPKKVDLNRPMESYIHYGVGPHTC

LGGEASKVALTAMLRVVGRLKNLRRAPGPQGELKKIPRDHGFYTYMRADETSLYALPMRSDLSRKSLRAM

FLDIGMIDMVWPFYHKIEFTMAKSAIE

#109 oxygenase not a P450

>BAE57912.1 [Aspergillus oryzae]

MLRRISTNFKKSKNDRETKQNGTQNGAQNGTQVNGDKRQSKVSPARKSADQEPSRKAANGASVFGKYAQV

LHASQSPLPNQTGDGATFEQRHGSLVQDLKSLHLEDAATLKQLSMNKIKGVPVDDKTMLMEKIIQIASSL

PDNSENRTKATNLFLNQLWDSLPHPPLSYVGPEYSYRSADGSNNNPTLPWLGAANTPYARSIAPLTIQPG

GLPDAGLVFDSLFAREKFNPHPNKVSSLFFDWASLIIHDIFQTDHANPHINKTSGYLDLSILYGDVQEEQ

DLIRTHRDGKLKPDSFSEPRLQAFPAACCVMLVMLNRFHNYVVEQLAEINENGRFTKPSPDLSEEKAKKA

WAKYDEDLFQTGRLITCGLYINITLYDYLRTIVNLNRVNSTWCLDPRAQMEGNDPTPSGLGNQCSVEFNL

AYRWHSAISANDEKWTEQIYEELMGKPAKDVTVLDLKKGLGKYAMGLSKDPSERTFAHLKRQEDGTFKDE

ELVSILANAIEDVAGSFGARNVPKCLRAVEIMGIEQARSWNVGSLNEFRKFFDLKPYERFEDINSDEEVV

EALRHLYGHPDYVELYPGIVAEDAKQPMVPGVGIAPTYTISRAVLSDAVALVRGDRFYTVDYNPRNLTNW

GYNEVRYDLNVNQGCVFYKLATRAFPNWFKSDSIYAHYPMTIPSENRNIMKDLGRESDFSYERPSFTPPH

VNLVSYPNVKLALEREEDFRVVWNGNTPLASAKGGDDFWSKSLDNDQWRNSIKEFYEDITAKLLQEKSGN

LAGLKQVDITRDIGNLAPVHFASKLFSLPLKTKENSRGVFTDNETFMSMAVIFTSIFFDVDKTKSFSLHH

AARAVAEQLGHSVENHVKSINSPSFLSGIIGNRRNDHNALKEYGDQLIKKLLESGLGVSDVTYSQVLPAA

VAMVHNQAQMFTQIIDYYLSEGKKHLPEINRLSKEDSKDSEDKLMRYCLEGFRLNGTFGSYREAQTDLSM

TEETGNVNIKRGDRVFVGAVKANRDPQVFPDPNEVHLDRPLESYIQYGLGPHTGLGKETTLLALTSMLRV

VGGLDNLRRAPGPQGELKKIHREGGYYVYLREDWGSYSPFPTTFKVHFDGAIPAPKKRLTYLGN

#140 oxygenase not a P450

>BAE60972.1 [Aspergillus oryzae]

MGWLSTTTKSSWNPFSTGSSGSSLDSGGSLASLAFGSKDQKWFQSVLSKFGFNGVLATVLSFFLYALYLK

FVSHDEKGLQTLIKEKASQYLGKIPGLNKLSFLKGHLPLGKFSKSLIPKATKGALGSIAGSSGGILSKLN

PFNWKIFNKRKIAEEEDDMRYQAGEPYGDPEVLAPTLRDDLKAVGLKAGVQDLKVLLDVVKNKGKPIDDR

DLTMEKLIAIVSSLPRNSKAREKLTGVLIDTLWQSLPHPPMTYLGNKYQYRTPDGSYNNPLQPDLGKAGS

PYARNVPKLKHMHGVPPDPGLLFDLLMARSDETFKENPAGLSSVLFYHATIIIHDIFRSNRFDPNISDTS

SYLDLAPLYGSSLEDQMKVRTKVRGLLKPDTFSEKRLIGMPPGVNAILVMYNRFHNYVADNLLKINEGGR

FSLPATKSEEDKKAALAKQDEDLFQTARLVTNGLYVNISLHDYIRGLANVHHSSSDWTLDPRVKINKIFD

SEGVPRGIGNQVSVEFNLLYRFHSIISRRDEKWMNEFFADIFGQDKKVDQLTPQEFIQGLYRFEQSIPED

PSEREFGGLKRGENGKFSDADLVQLMKDSMEDPAGCFGARMVPKALRVIEILGIIQARKWQLASLNETRD

FFKLKRHETFEDVNSNHEIADLLRKLYDDPDMVEMYPGLVGHGGCTPYTVGRAVFSDAVTLVRSDRFLTI

DYTASNLTCWGYNEVQQDYDILGGSMFHKLFQRALPNWFPYNSLHITQPMYTRKMNEQIAREIGTIDEYT

LDDPSPPPKTVIVTKHSTITKLSKDQANFRVIWAKYLNEMIPGRDFSGYMLLGDKPANTDQKTLVKEILY

SPAEFVQLLSETAVSVAKEQLATETLNLTSELHQVDIVRDVAIPMVTRILADLFCLDLKTPENPNGTYNV

AELYKYIIDVRIFGFNNDDPGLALQRRKWAREGAESLTKTTLRVVSNLPASEKSGKGIVKGAVSTAKSIA

SKIPLVGKLVGDGKGVEGQSTSGSLRWYGYNVAKELIASGKTPAEVADISWMNAVGGVGATIGVFTDVLN

YFLQDENSHHWEEIQKLAASSDLESSNKSLRQYVLEAQRLTSTQRSIRLCAGKAVIDGQSFEPGNLVICL

LGAACKDPDAVPDPEAFKLDRPSSAYIHFNVGPHECLGREIALSCITSLVRVCAGLKNLRAAPGQMGVLK

SITTGTEKHFLNDSWSTLTVDPTTWKIHFEGQGQGIHHPPKIPVTAGRDLNALSNALKKQHQDKLQETVS

KVANGVTAPLTKLIPSNGPSNGASTPGHLPLPINPFQNGNGNSNGVANGNTHPSLLHQAVSSAAAIPQHA

LGTVHDVAHNTVGHLPGGQQVTDFTHGLVHPFAGAVSPGQTQTPPQGQTQPHQGNWFFLPHGMENAAKQV

PGHLFGQTA

#149

79% to EAL89712.1 fatty acid oxygenase PpoA not a p450

>BAE58228.1 [Aspergillus oryzae]

MEGARLRSSVGLPRMVTKPTVIEDNGKKLNLKAGQHILCNLVSASMDPTSFPEPEKVRLDRDMDLYAHFG

FGPHQCLGIGLCKLALTTMLKVIGRLDNLRRAPGPQGQLKKLSGPGGIAKYMNPNQSGFSPFPTSMKIQW

DGELPQVER