Arabidopsis Cytochrome P450 ESTs

(by family)

Revised March 29, 2000 11 more ESTs identified 10/255 = 4% are not yet 
identified.  Currently there are 255 ESTs in this table.  Because some of these 
ESTs are from the same sequence, there are no more than 7 separate genes in 4 
families represented by these 10 ESTs

Revised Sept. 8, 1999 7 more ESTs identified 20/255 = 8% are not yet identified
Currently there are 254 ESTs in this table.  Because some of these ESTs are from the same 
sequence, there are no more than 13 separate genes in 8 families represented by these 20 
ESTs

Revised Aug. 3, 1999 6 more ESTs identified 26/254 = 10% are not yet identified
Currently there are 254 ESTs in this table.  Because some of these ESTs are from the same 
sequence, there are no more than 18 separate genes in 9 families represented by these 26 
ESTs

Revised May 18, 1999 2 more ESTs identified 32/254 = 13% are not yet identified
Currently there are 254 ESTs in this table.  Because some of these ESTs are from the same 
sequence, there are no more than 22 separate genes represented by these 32 ESTs

Revised April 26, 1999 9 more ESTs identified 32/252 = 13% are not yet identified
Currently there are 252 ESTs in this table.  Because some of these ESTs are from the same 
sequence, there are no more than 22 separate genes represented by these 32 ESTs

Revised April 16, 1999 2 more ESTs identified 41/252 = 16% are not yet identified
Currently there are 252 ESTs in this table.  Because some of these ESTs are from the same 
sequence, there are no more than 26 separate genes represented by these 41 ESTs

Revised April 15, 1999 4 more ESTs identified 43/252 = 17% are not yet identified
Currently there are 252 ESTs in this table.

Revised April 13, 1999 3 more ESTs identified 47/252 = 19% are not yet identified
Currently there are 252 ESTs in this table.

Revised April 6, 1999 3 more ESTs identified 50/252 = 20% are not yet identified
Currently there are 252 ESTs in this table.

Revised April 2, 1999 13 more ESTs identified 50/249 = 20% are not yet identified
Currently there are 249 ESTs in this table.

Revised Mar. 26, 1999  6 more ESTs identified 25% of ESTs are still not identified
Currently there are 247 ESTs in this table.

Revised Mar. 3, 1999  18 more ESTs identified 27% of ESTs are still not identified

Completely revised April 21, 1998 (new EST search done) Search strategy was
(P450 AND arabidopsis[LIB LIBX CLIN]) NOT (reductase OR D64052)
This last exclusion is to prevent matches to a P450-like TATA Binding Protein.
This search resulted in 217 hits, 13 were new.  My old list had 230 hits, so 26 of the old 
numbers were not found on this search.  I know that three have been deleted from 
genbank (T04452, T04714 and T13009).  I don't know why.

The assignments made below are subject to change when longer fragments become 
available.  I have tried to be as careful as possible in making assignments, but I cannot 
foretell the future.  All percents refer to protein sequence identities.

When clone names indicate the same clone has been sequenced from both ends, these 
fragments are included in one sequence (example: T04186 and T04714) 
T7 at the end of a clone name indicates the T7 primer was used for sequencing.
XP at the end of a clone name means the M13 reverse primer was used.

Comparisons between percent identities are not meaningful unless the fragments cover the 
same region of the P450 sequence.  The C-terminal is more highly conserved than the N-
terminal and this is more conserved than the middle of the sequences.  30% identity in the 
middle may be significant while 50% might be low at the C-terminal.

163 fragments were returned from a search of the EST database with the boolean
expression (P450 AND Arabidopsis[LIB]) NOT reductase on 7/9/96.
Another search was done May 19, 1997 and the new ESTs are included.  The most recent 
search was April 14, 1998 which only found 13 new hits.

A large number of hits to P450 as a string search were to a sequence D64052 from tobacco.  
This has been published as a P450-like TBP sequence, however, the sequence has a blastn 
score of e-311 with a rRNA and therefore it is an rRNA related sequence and not a P450. 
All matches to D64052 have been left out.